Silver film is commonly used to examine the structure and composition of molecules due to its surface-enhanced Raman scattering (SERS) effect. A simple method for preparing SERS silver films is proposed in this paper by electrodeposition in an amide-based liquid mixture (urea-acetamide-LiBr). The electrochemical behavior of silver in urea-acetamide-LiBr mixture was studied by cyclic voltammetry (CV) and chronoamperometry. It was found that the nucleation mechanism of silver shifts to instantaneous nucleation under diffusion control with increasing overpotential and temperature. The energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD) investigations confirmed that the obtained deposits are pure silver. Scanning electron microscopy (SEM) analysis showed that the morphology of the silver deposits depends on the deposition potential and temperature. Rhodamine 6G was used as probe molecules for SERS experiments, showing that these new active substrates have high sensitivity to SERS response with high enhancement factors, of approximately 107, and enable Raman scattering even for diluted solutions. surfaceenhanced surface enhanced (SERS effect amidebased amide based ureaacetamideLiBr. ureaacetamideLiBr urea acetamide LiBr . (urea-acetamide-LiBr) CV (CV chronoamperometry temperature EDS (EDS Xray X ray XRD (XRD SEM (SEM G experiments factors 107 solutions (urea-acetamide-LiBr 10 1

This research aims to develop a precise and efficient dispersive solid-phase microextraction (DSPE) technique using third-generation polyamidoamine (PAMAM) dendrimer-grafted nanoparticles to quantify benzoylurea pesticides in green tea, black tea, and oolong tea infusions. The extraction efficiency is optimized through single-factor adjustments of key parameters, including adsorbent quantity (5-20 mg), adsorption duration (5-20 min), desorbent identity (methanol, acetonitrile), volume (100-600 μL), sodium chloride concentration (0-10%, m/v), and sample solution pH (3-8). The optimized method demonstrates linearity in the range of 2-500 μg L-1, with limits of detection (LODs) ranging from 0.6-1.5 μg L-1 and limits of quantification (LOQs) from 2.0 5.0 μg L-1. Recovery rates of 81.2-96.0% are achieved, with intra-day relative standard deviations (RSDs) of 1.4-2.8% and inter-day relative standard deviations of 2.0-3.9%, indicating high accuracy and repeatability. The successful application of detecting residues in commercial tea samples underscores the strong potential of the technique to determine benzoylurea analyte levels. solidphase solid phase DSPE (DSPE thirdgeneration third generation PAMAM (PAMAM dendrimergrafted dendrimer grafted infusions singlefactor single factor parameters 520 5 20 (5-2 mg, mg , mg) min, min min) methanol, methanol (methanol acetonitrile, acetonitrile acetonitrile) 100600 100 600 (100-60 μL, μL μL) 010%, 010 0 10%, 10 (0-10% m/v, mv m/v m v m/v) 38. 38 3 8 . (3-8) 2500 2 500 2-50 L1, L1 L 1, 1 LODs (LODs 0.61.5 0615 0.6 1.5 6 0.6-1. L- LOQs (LOQs 2. 50 5. L1. 1. 81.296.0% 812960 81.2 96.0% 81 96 81.2-96.0 achieved intraday intra day RSDs (RSDs 1.42.8% 1428 1.4 2.8% 4 1.4-2.8 interday inter 2.03.9%, 2039 3.9%, 9 2.0-3.9% repeatability levels 52 (5- 10060 60 (100-6 010% 01 10% (0-10 (3-8 250 2-5 61 0.61. 061 06 0. 15 0.6-1 296 81.296.0 81296 812 81. 960 96.0 81.2-96. 42 1.42.8 142 14 28 2.8 1.4-2. 03 2.03.9% 203 39 3.9% 2.0-3.9 (5 1006 (100- (0-1 (3- 25 2- 0.61 0.6- 29 81.296. 8129 96. 81.2-96 1.42. 1.4-2 2.03.9 3.9 2.0-3. ( (100 (0- (3 81.296 81.2-9 1.42 1.4- 2.03. 3. 2.0-3 (10 (0 81.29 81.2- 2.03 2.0- (1

Abstract To further improve the strike accuracy and penetration power of explosively-formed projectile (EFP) after long-distance flight, a polygonal charge capable of forming EFP with tail fins was designed during the study. A model for calculating the EFP velocity was derived from the effective charge and detonation projection theories with the integration of calculus. The circumferential velocity distributions of EFP formed using polygonal charges were obtained using this model, and the mechanisms underlying the formation of tail fins were elucidated. The feasibility of using this polygonal charge to form tail fin EFP was verified by conducting multi-media soft recovery tests and numerical simulations. Furthermore, the feasibility of forming tail fin EFP using polygonal charges with different numbers of sides and the effects of the structural parameters of a hexagonal charge on the formation of tail fin EFP were investigated by simulation. The ranges of optimal values for the structural parameters of a hexagonal charge capable of forming high-performance tail fin EFPs were determined. explosivelyformed explosively (EFP longdistance long distance flight study calculus elucidated multimedia multi media simulations Furthermore simulation highperformance high performance determined

Abstract In this paper, the penetration resistance of 6061 aluminum plates to explosively formed projectiles (EFPs) at the incidence angles of 0°, 15°, 30°, and 45° was studied. The penetration process was roughly divided into three typical stages—the plastic flanging stage of the target, the righting stage of the projectile, and the plug formation stage of the target. Through the energy analysis, the energy consumption of the aluminum plate increased with the increase in the incidence angle during the entire penetration process. Through the microscopic analysis of the perforated wall surface of the aluminum plate, it was found that there were distinct boundaries and differences between the plastic reaming zone and the punching plug fracture zone.. Overall, four kinds of classification modes were proposed for the damage of the aftereffect plate, which provides a method to evaluate the aftereffect of EFP oblique penetration into 6061 aluminum plates. paper 606 EFPs (EFPs 0 0° 15 15° 30 30° 45 studied stagesthe stages target projectile zone. Overall 60 1 3 4 6

Abstract To study the propagation and evolution of one-dimensional elastoplastic waves within the system under rectangular, trapezoidal, and half-sine incident waves, leveraging elastoplastic incremental wave theory and numerical simulation calculations, the research qualitatively investigates the distribution and evolution of the stress field within the specimen, the impact of Bar 2 parameters, and the deformation characteristics of Bar 2 under these three types of incident waves. The findings indicate: Regardingthe overall stress uniformity of Bar 2 during the entire loading phase, the rectangular incident wave is the most effective, while the half-sine and trapezoidal waves are more advantageous for analyzing the stress conditions in Bar 2 during the early loading phase. The diameter and dimensionless density of the specimen significantly influence the stress non-uniformity, showing an approximate negative and positive correlation, respectively. onedimensional one dimensional halfsine half sine calculations parameters indicate phase effective nonuniformity, nonuniformity non uniformity, non-uniformity correlation respectively

ABSTRACT Gaussian mixture probability hypothesis density (GM-PHD) filtering often assumes a uniform distribution of clutter in the observation area. However, in practice, clutter is often unknown and non-uniform, necessitating accurate estimation of its spatial distribution, non-uniformity, and temporal variations. To address this problem, we proposed a modified GM-PHD filtering method with clutter density estimation for multiple target tracking. In the proposed method, first, potential target measurements within the tracking gate are eliminated to obtain the clutter measurement set. Next, the clutter density around each target is estimated. Finally, the estimated clutter density is incorporated into GM-PHD filtering, to estimate the target state and clutter density in complex clutter environments. Simulation results demonstrated that the proposed filtering method improves the performance of the GM-PHD filter in multi-target tracking scenarios with unknown clutter density. GMPHD GM PHD (GM-PHD area However practice nonuniform, nonuniform non uniform, non-uniform nonuniformity, nonuniformity uniformity, uniformity non-uniformity variations problem first set Next Finally environments multitarget multi

Abstract Background The underlying mechanism of chronic pain involves the plasticity in synaptic receptors and neurotransmitters. This study aimed to investigate potential roles of Neuroligins (NLs) within the spinal dorsal horn of rats in a newly established Bone Cancer Pain (BCP) model. The objective was to explore the mechanism of neuroligin involved in the occurrence and development of bone cancer pain. Methods Using our rat BCP model, we assessed pain hypersensitivity over time. Quantitative real-time polymerase chain reaction and Western blot analysis were performed to investigate NL expression, and NLs were overexpressed in the rat spinal cord using lentiviral vectors. Immunofluorescence staining and whole-cell patch-clamp recordings were deployed to investigate the role of NLs in the development of BCP. Results We observed reduced expression levels of NL1 and NL2, but not of NL3, within the rat spinal cord, which were found to be associated with and essential for the development of BCP in our model. Accordingly, NL1 or NL2 overexpression in the spinal cord alleviated mechanical hypersensitivity of rats. Electrophysiological experiments indicated that NL1 and NL2 are involved in BCP via regulating γ-aminobutyric acid-ergic interneuronal synapses and the activity of glutamatergic interneuronal synapses, respectively. Conclusions Our observations unravel the role of NLs in cancer-related chronic pain and further suggest that inhibitory mechanisms are central features of BCP in the spinal dorsal horn. These results provide a new perspective and basis for subsequent studies elucidating the onset and progression of BCP. neurotransmitters (NLs (BCP model time realtime real vectors wholecell whole cell patchclamp patch clamp NL3 Accordingly γaminobutyric γ aminobutyric acidergic acid ergic respectively cancerrelated related

Abstract Background To explore the median effective dose (ED50) and 95% effective dose (ED95) of remimazolam besylate combined with alfentanil for adult gastroscopy. Methods This prospective studyenrolled 31 patients scheduled to painless gastroscopy at Anhui No. 2 Provincial People's Hospital between April and May, 2022. 5 µg.kg−1 of alfentanil hydrochloride was used for pre-analgesia. The initial single loading dose of remimazolam besylate was 0.12 mg.kg−1, increased or reduced by 0.01 mg.kg−1 for the next patient with modified Dixon sequential method. The modified Observer's Assessment of Alertness/Sedation Scale (MOAA/S) was used to assess sedation. Results Combined with alfentanil, the ED50 of remimazolam besylate was 0.147 mg.kg−1 (95% CI: 0.138-0.160 mg.kg−1) and ED95 0.171 mg.kg−1 (95% CI: 0.159-0.245 mg.kg−1). The induction time after injection of remimazolam besylate was 70 ± 25 s, with the anesthesia recovery time and the observation time in resuscitation room 5.13 ± 2.13 min and 2.32 ± 1.6 min, respectively. Twenty nine patients’ vital signs were within acceptable limits during gastroscopy. Conclusions The ED50 of remimazolam besylate combined with alfentanil for painless gastroscopy was 0.147 mg.kg−1, and the ED95 was 0.171 mg.kg−1. ED (ED50 95 (ED95 3 No Peoples People s May 2022 µgkg1 µgkg µg kg 1 µg.kg− preanalgesia. preanalgesia pre analgesia. analgesia pre-analgesia 012 0 12 0.1 mgkg1 mgkg mg 001 01 0.0 mg.kg− method Observers Observer AlertnessSedation Alertness Sedation MOAA/S MOAAS MOAA S (MOAA/S sedation ED5 0147 147 0.14 (95 CI 0.1380.160 01380160 0.138 0.160 138 160 0.138-0.16 ED9 0171 171 0.17 0.1590.245 01590245 0.159 0.245 159 245 0.159-0.24 . 7 513 13 5.1 213 2.1 232 32 2.3 16 6 1. respectively (ED5 9 (ED9 202 µg.kg 0. 00 mg.kg 014 14 (9 1380 0.1380.16 0138016 0138 0.13 0160 0.16 0.138-0.1 017 17 1590 0.1590.24 0159024 0159 0.15 0245 0.24 15 24 0.159-0.2 51 5. 21 2. 23 (ED 20 ( 0.1380.1 013801 013 016 0.138-0. 0.1590.2 015902 015 024 0.2 0.159-0. 0.1380. 01380 0.138-0 0.1590. 01590 02 0.159-0 0.1380 0.138- 0.1590 0.159-

Abstract Objectives: The role of Epoxide Hydrolase-4 (EPHX4), a member of epoxide hydrolase family, has not been investigated in cancer. The purpose of this article is to explore the application value of EPHX4 in laryngeal cancer and its relationship with immune infiltration. Methods: We observed that EPHX4 expression and its survival assays in laryngeal cancer specimens based on The Cancer Genome Atlas (TCGA) cohorts. We also analyzed the correlation between immune cell infiltration levels and EPHX4 gene copy number in laryngeal cancer. Finally, we conducted in vitro assay to evaluate the functions of EPHX4 in laryngeal cancer cell line. Results: EPHX4 is highly expressed in laryngeal cancer specimens and has a poor prognosis. EPHX4 related immune cell analysis showed that it participated in NK Natural killer cell mediated cytotoxicity. Finally, Cell experiments indicate that EPHX4 could promote laryngeal cancer cell line proliferation, colony formation and invasion. Conclusions: Our research results suggest that EPHX4 may be a potential immunotherapy target for laryngeal cancer. The nominated immune signature is a helpful and promising prognostic indicator in laryngeal cancer. Levels of Evidence: Level 3. Objectives Hydrolase4 Hydrolase 4 Hydrolase- EPHX4, EPHX , (EPHX4) family Methods TCGA (TCGA cohorts Finally Results prognosis cytotoxicity proliferation invasion Conclusions Evidence 3 (EPHX4 (EPHX

Abstract Objective Dizziness or vertigo in older population frequently presents in clinical settings, yet its etiology remains elusive. The objective of this study was to delineate global trends and identify frontiers in research concerning dizziness or vertigo among older population. Methods We searched the research literature published from 2003 to 2022 on older population with dizziness or vertigo using two databases from the Web of Science Core Collection. A bibliometric and visualization analysis was conducted. Bibliometric tools facilitated co-authorship, co-citation, and keyword co-occurrence analyses, encompassing countries or regions, institutions, authors, journals, and references. Results The analysis included 1322 publications authored by 6524 individuals from 2244 institutions across 67 countries or regions, spanning 92 subject categories. A steady increase in publications was noted from 2003 to 2022. The University of Munich, Harvard University, and the University of California System emerged as leading institutions with the highest publication outputs. The United States, Germany, and China were predominant in publication counts. Eva Grill was identified as the most prolific author. Otology & Neurotology and Geriatrics & Gerontology emerged as the most prolific journal and subject category, respectively. The most prevalent keywords were “dizziness”, “vertigo”, “falls”, and “geriatric”, with “management”, “gait”, and “association” recognized as the principal research hotspots. Conclusion This study provides a systematic analysis of global scientific research on older population dizziness/vertigo, revealing significant advancements in understanding over the past two decades. Management, gait, and association have emerged as the primary research focuses on recent years. These findings offer valuable insights for directing current research efforts to capture prevailing trends and explore new frontiers in this field. settings elusive 200 202 Collection conducted coauthorship, coauthorship co authorship, authorship co-authorship cocitation, cocitation citation, citation co-citation cooccurrence occurrence analyses regions authors journals references 132 652 224 6 9 categories Munich outputs States Germany counts author category respectively dizziness, , “dizziness” vertigo, “vertigo” falls, falls “falls” geriatric, geriatric “geriatric” management, management “management” gait “gait” “association hotspots dizzinessvertigo dizziness/vertigo decades Management years field 20 13 65 22 “dizziness “vertigo “falls “geriatric “management “gait 2 1

BACKGROUND: It is commonly acknowledged that cooperatives play important social functions that raise the standard of living for their members, particularly those who originate from rural, and low-income backgrounds. AIM: This article aims to measure the impacts of cooperatives membership on household income taking Zanzibar as a case study. SETTING: The data used were directly collected from 217 cooperative members and 83 non-cooperative members. METHOD: Descriptive statistics were used to analyse the demographic characteristics of the respondents. The probit model and propensity score matching (PSM) was used to analyse the impacts of cooperative membership on household income. RESULTS: The probit model findings show that there are four statistically significant factors affecting cooperatives membership, including gender, educational level, land ownership, and access to credit. In addition, PSM findings reveal that there is a disparity in income level between cooperative members and non-members. On average, cooperative members are able to generate more income than non-cooperative members by 28% per year. CONCLUSION: The study concludes that, in order to expand the observed benefits to the population, cooperative growth needs proper backing. Because poverty has many different dimensions, it's crucial to expand the organisations that help the poor while also utilising other support services to reduce it. CONTRIBUTION: The article serves as first empirical evidence to be conducted in Zanzibar, Tanzania. The findings will facilitate the amendment of the cooperative context, including tax reduction, extending loans and grants, and other favourable working conditions necessary for supporting the development of cooperative society.

Abstract Objectives We aimed to explore the heterogeneity and differentiation trajectories of epithelial cells and NK/T-cells in Laryngeal Squamous Cell Carcinoma (LSCC). Methods We downloaded the GSE150321 data set containing LSCC01 and LSCC02 samples single cell RNA data from Gene Expression Omnibus. The UMAP analysis was performed to identify the cell subpopulations and cell locations of subpopulations. Seurat package was used to analyze the differential expression of genes. The function of differential expression genes was analyzed using DAVID database. The monocle2 package was used to analyze differentiation trajectories. We used the CellChat package to observe the signaling pathways and ligand-receptor pairs for epithelial cells and NK/T-cells. Results All the LSCC cells were divided into 16 subpopulation that included 7 epithelial cell subsets, 3 T-cell subsets. The function analysis indicated that epithelial cells and NK/T-cells mainly participated in different process, such as cell cycle, immune response, and cell migration. Then, the results of differentiation trajectory indicated that the ability of migration, and the activation of the immune system increases, while the ability of apoptosis, and glucose metabolic process decreases as pseudotime. Migration-related epithelial cells act on all T-cells via the CNTN2-CNTN2 ligand-receptor pair, which suggested that CNTN2 might be an important biomarker for regulating migration of epithelial cells. Conclusions Our study characterized the heterogeneity of LSCC, which provided novel insights into LSCC and identified a new mechanism and target for clinical LSCC threapies. Evidence IV. NK/Tcells NKTcells NK/T NK T LSCC. . (LSCC) GSE GSE15032 LSCC0 Omnibus database monocle ligandreceptor ligand receptor NK/Tcells. 1 subsets Tcell cycle response Then increases apoptosis pseudotime Migrationrelated Migration related Tcells CNTN2CNTN2 CNTNCNTN CNTN CNTN2-CNTN pair threapies IV NKT (LSCC GSE1503 CNTN2CNTN GSE150 GSE15 GSE1

Abstract Objective: Chronic Rhinosinusitis with Polyps (CRSwNP) is characterized by high heterogeneity and postoperative recurrence rate. This study aims to explore the clinical significance of tissue Leukocyte-Specific Transcript 1 (LST1) in predicting CRSwNP recurrence. Methods: We enrolled 62 CRSwNP patients including 30 primary CRSwNP and 32 recurrent CRSwNP patients, and 40 Healthy Controls (HC). Tissue samples were collected. Tissue LST1 expression was assessed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Western Blotting (WB) and Immunofluorescence (IF) staining. The predictive values of LST1 expression for CRSwNP postoperative recurrence were assessed through the Receiver Operating Characteristic (ROC) curves. Results: The tissue levels of LST1 were significantly increased in the CRSwNP group than the HC group, especially in the recurrent group, and the elevated LST1 mRNA levels were positively correlated with the peripheral eosinophil percentages, tissue eosinophil counts and percentages. IF staining results showed that the LST1 protein levels were higher in CRSwNP patients, especially in the recurrent patients than in the HC group. ROC curves highlighted that tissue LST1 levels were associated with recurrent CRSwNP and exhibited a higher predictive ability for postoperative CRSwNP recurrence. Conclusion: This was the first report suggesting that LST1 expression was upregulated and associated with mucosal eosinophil infiltration and CRSwNP recurrence. Tissue LST1 could be a promising biomarker for predicting postoperative recurrence in CRwNP patients. Objective (CRSwNP rate LeukocyteSpecific Leukocyte Specific LST (LST1 Methods 6 3 4 HC. . (HC) collected TranscriptionPolymerase Transcription Polymerase RTPCR, RTPCR RT PCR , (RT-PCR) WB (WB (IF (ROC Results percentages Conclusion (LST (HC (RT-PCR

Rapid recognition of the sources of drugs can provide some valuable clues and the basis for determining the nature of the case. A novel recognition method was put forward to identify the sources of heroin drugs rapidly and non-destructively by using a hand-held near infrared (NIR) spectrometer and a multi-layer-extreme learning machine (ML-ELM) algorithm. In contrast to traditional linear discriminant analysis (LDA), support vector machine (SVM) and extreme learning machine (ELM) algorithms, the accuracy, sensitivity and specificity were the highest for the proposed ML-ELM algorithm. The prediction accuracy of the ML-ELM algorithm was 25.33, 20.00, 17.33% higher than that of LDA, SVM and ELM algorithm, respectively, for 4 cases. The ML-ELM models for recognizing the different sources of heroin drugs had the best generalization ability and prediction results. The experimental results indicated that the combination of the hand held NIR technology and ML-ELM algorithm can recognize the different sources of heroin drugs rapidly, accurately, and non-destructively on the spot. case nondestructively non destructively handheld (NIR multilayerextreme multi layer MLELM ML (ML-ELM LDA , (LDA) (SVM (ELM algorithms 2533 25 33 25.33 2000 20 00 20.00 1733 17 17.33 respectively cases accurately spot (LDA 253 2 3 25.3 200 0 20.0 173 1 17.3 25. 20. 17.