ABSTRACT The bacterial canker, caused by Xanthomonas citri pv. viticola, is the most important bacterial disease of grapevines cultivated in tropical areas. The objectives of this study were to select grape hybrids resistant to bacterial canker based on genotypic values and to evaluate the response of seedless grape hybrids to artificial infection with bacterial canker. Two experiments were conducted. The field experiment (Experiment I) was conducted with 569 hybrids, evaluated for symptoms of bacterial canker, in the absence of an experimental design, using a single individual of each genotype. Experiment II was conducted in a greenhouse, where ten seedless grape hybrids were evaluated in terms of incubation period, incidence, severity, and area under the progress curve of bacterial canker disease incidence, in a completely randomized design. The use of the residual maximum likelihood/best unbiased linear prediction methodology allowed the selection of 40 grape hybrids with lower genotypic values for resistance to bacterial canker. The CPATSA 49.25 and CPATSA 49.86 hybrids showed greater resistance to bacterial canker in all epidemiological parameters evaluated under greenhouse conditions. pv viticola areas I 56 design genotype period incidence severity likelihoodbest likelihood best 4 4925 49 25 49.2 4986 86 49.8 conditions 5 492 2 49. 498 8

Abstract This study validated the content of an instrument designed to assess the knowledge, involvement (attitudes) and management (practice) of dentists relative to sickle-cell disease (KAPD-SCD). The instrument consisted of five domains composed of a total of thirteen items: I. Dentist’s self-assessment relative to sickle-cell disease; II. Dentist’s knowledge of the repercussions of sickle-cell disease on the stomatognathic system; III. Dentist’s knowledge of the complications of sickle-cell disease in the stomatognathic system; IV. Dentist’s knowledge concerning the dental management of sickle-cell disease patients; and V. Dentist’s involvement in an approach to sickle-cell disease. Twelve experts assigned scores to each item of the instrument. The criteria were clarity, understanding and appropriateness, leaving open fields for comments. Descriptive and content analyses of the data were made. Each expert analyzed 39 assessment units. The percentages considered for agreement were high (>80%), medium (70%-80%), or low (<70%), and each item was maintained or revised according to the percentage observed. There was high consensus in 74% of the assessment units (the corresponding items were maintained), medium consensus in 24% of them (the corresponding items were revised), and disagreement in 2% of them, namely as regards the “appropriateness” of item 5 (“Are there oral complications in sickle-cell disease?”), which was revised. The final version of the instrument had 16 items for different applications such as in the clinical care program, teaching program, or research program, with different cut-off scores for each application. In conclusion, the level of agreement among experts showed evidence of the content validity of the instrument. attitudes (attitudes practice (practice sicklecell sickle cell KAPDSCD. KAPDSCD KAPD SCD . (KAPD-SCD) I Dentists Dentist s selfassessment self II system III IV patients V clarity appropriateness comments made 3 >80%, 80 >80% , (>80%) 70%80%, 7080 70% 80% 70 (70%-80%) <70%, <70% (<70%) observed 74 maintained, maintained) 24 revised, revised) 2 “appropriateness Are disease, disease?”) 1 program cutoff cut off application conclusion (KAPD-SCD 8 >80 (>80% 70%80% 708 7 (70%-80% <70 (<70% disease?” >8 (>80 70%80 (70%-80 <7 (<70 disease? > (>8 70%8 (70%-8 < (<7 (> (70%- (< ( (70% (70 (7

ABSTRACT Objective: In order to study the scenario of respiratory infections in pediatrics after the emergence of SARS-CoV-2 in Brazil, this study aimed to compare characteristics of children admitted for SARS or upper airway infection caused by either RSV or SARS-CoV-2. Methods: This was a cross-sectional study involving children up to 48 months of age admitted to a tertiary pediatric hospital with a diagnosis of SARS or upper airway infection between April of 2020 and April of 2021. Respiratory secretion samples were collected 2-5 days after hospitalization, and antigen/PCR tests for viral etiologies were performed. In this analysis, patients with laboratorial diagnosis of SARS-CoV-2 and/or RSV were selected, and their clinical and epidemiological characteristics were compared using logistic regression. Results: Our sample initially comprised 369 participants. SARS-CoV-2 and RSV infections were confirmed in 55 (15%) and 59 children (16%), respectively. Mean age was 12 months (0-48 months), and 47 were female. The following characteristics were significantly more frequent in patients with RSV when compared with those with COVID-19: younger age (OR = 0.94; 95% CI: 0.90-0.98); lower frequency of fever (OR = 0.18; 95% CI: 0.05-0.66); and more frequent upper airway symptoms: cough (OR = 7.36; 95% CI: 1.04-52.25); and tachypnea (OR = 6.06; 95% CI: 1.31-28.0). Conclusions: Children with RSV-related SARS were younger, had lower frequency of fever at admission, but had a higher frequency of signs of upper airway infection and lower systemic inflammation when compared with children hospitalized for COVID-19 during the first year of the pandemic. Objective SARSCoV2 SARSCoV CoV 2 SARS-CoV- Brazil SARSCoV2. 2. Methods crosssectional cross sectional 4 202 2021 25 5 2- hospitalization antigenPCR antigen PCR performed analysis andor selected regression Results 36 participants 15% 15 (15% 16%, 16 16% , (16%) respectively 1 048 0 (0-4 months, months) female COVID19 COVID 19 OR 0.94 094 94 95 CI 0.900.98 090098 0.90 0.98 90 98 0.90-0.98) 0.18 018 18 0.050.66 005066 0.05 0.66 05 66 0.05-0.66) symptoms 7.36 736 7 1.0452.25 1045225 1.04 52.25 04 52 1.04-52.25) 6.06 606 6 06 1.3128.0. 131280 1.31 28.0 . 31 28 1.31-28.0) Conclusions RSVrelated related admission COVID-1 pandemic SARS-CoV 20 3 (15 (16% (0- COVID1 0.9 09 9 900 0.900.9 09009 090 098 0.90-0.98 0.1 01 050 0.050.6 00506 005 0.0 066 0.6 0.05-0.66 7.3 73 0452 1.0452.2 104522 104 1.0 5225 52.2 1.04-52.25 6.0 60 3128 1.3128.0 13128 131 1.3 280 28. 1.31-28.0 COVID- (1 (16 (0 0. 0.900. 0900 0.90-0.9 0.050. 0050 00 0.05-0.6 7. 045 1.0452. 10452 10 1. 522 52. 1.04-52.2 6. 312 1.3128. 1312 13 1.31-28. ( 0.900 0.90-0. 0.050 0.05-0. 1.0452 1045 1.04-52. 1.3128 1.31-28 0.90-0 0.05-0 1.045 1.04-52 1.312 1.31-2 0.90- 0.05- 1.04-5 1.31- 1.04-

Abstract Background: Electroconvulsive therapy (ECT) is a part of Brazilian Nursing History that needs to be known to understand the evolution of knowledge and practice following the Psychiatric Reform. Objective: To identify nursing care for inpatients undergoing ECT at a Brazilian psychiatric institution between 1980 and 1990. Methodology: Social-historical study based on written documents from a Brazilian psychiatric institution and oral statements from nursing professionals, analyzed according to Foucault’s framework. Results: Nursing care was defined based on everyday experiences and provided before, during, and after ECT. Nursing professionals had no professional training. They acted autonomously, administering ECT to calm agitated patients, or accompanied physicians’ interventions, providing care to reduce the side effects of ECT. Conclusion: Despite being part of the asylum-style care model, ECT included nursing care to avoid unwanted effects. Nursing professionals witnessed the decline of ECT in society. The present study describes aspects of ECT that are inconsistent with today’s practice.

Resumen Marco contextual: La terapia electroconvulsiva (TEC) forma parte del pasado de la enfermería que es necesario conocer para dar sentido a las transformaciones del conocimiento y la práctica tras la Reforma Psiquiátrica. Objetivo: Identificar los cuidados de enfermería en la aplicación de la terapia electroconvulsiva en pacientes ingresados en un instituto psiquiátrico brasileño entre 1980 y 1990. Metodología: Investigación histórico-social en documentos escritos de la institución y documentos orales producidos con profesionales de enfermería, interpretados según el referencial foucaultiano. Resultados: Los cuidados de enfermería se definieron por la experiencia diaria y se llevaron a cabo antes, durante y después de la terapia electroconvulsiva. El personal de enfermería no tenía formación profesional y actuaba por su cuenta, aplicando la terapia para calmar a los pacientes agitados o acompañando la aplicación del médico, proporcionando cuidados para reducir los efectos secundarios. Conclusión: A pesar del modelo de manicomio, la aplicación de la terapia electroconvulsiva contaba con cuidados de enfermería a los pacientes para evitar efectos no deseados. La enfermería ha seguido el declive de la TEC en la sociedad. Este estudio pone de manifiesto las características de la TEC que no se ajustan a la práctica contemporánea.

Resumo Enquadramento: A terapia electroconvulsiva (TEC) é parte do passado da enfermagem que precisa de ser conhecido, a fim de dar sentido às transformações nos saberes e práticas após a Reforma Psiquiátrica. Objetivo: Identificar os cuidados de enfermagem na aplicação da terapia electroconvulsiva em utentes internados num instituto psiquiátrico brasileiro entre 1980 e 1990. Metodologia: Investigação histórico-social em documentos escritos da instituição cenário e orais produzidos com profissionais de enfermagem, interpretados à luz do referencial foucaultiano. Resultados: Os cuidados de enfermagem foram definidos pela experiência quotidiana e realizados nos momentos pré, trans e pós a aplicação da terapia electroconvulsiva. A enfermagem não tinha formação profissional e agia por conta própria, aplicando a terapia para acalmar utentes agitados ou acompanhando a aplicação pelo médico, prestando cuidados para reduzir efeitos colaterais. Conclusão: Apesar do modelo manicomial, a aplicação da terapia electroconvulsiva contava com cuidados de enfermagem aos utentes para evitar efeitos indesejados. A enfermagem acompanhou o declínio da TEC na sociedade. Este estudo evidenciou características da TEC que não se adequam à prática contemporânea.

Abstract Background: Human Polyomaviruses such as MCPyV and HPyV6 are frequently found as part of healthy skin microbiota and have been associated with Merkel cell carcinoma (MCC), pruritic and dyskeratotic dermatoses, respectively. Their presence in other types of skin conditions varies greatly depending on lesion type and population. Objectives: To analyse comparatively the presence of MCPyV and HPyV6 in nonmelanoma skin cancers and healthy skin. Methods: The authors utilized qPCR techniques to quantify these pathogens in NMSC, premalignant diseases, and healthy skin of 87 patients. Results: MCPyV was detected in over 40% of samples, while HPyV6 was in 9.6%. MCPyV load was higher in squamous cell carcinomas (SCC) compared to basal cell carcinomas (BCC) (p = 0.016) and HPyV6 showed a higher percentage of infected cells in areas of low solar exposure as well as normal skin (p = 0.012). A fair agreement (kappa = 0.301) was found between MCPyV detection in lesions and their respective perilesional skin, indicating a random process of local dissemination of the virus. Study limitations: The lack of a larger sampling of different lesion types and protein expression analyses limits the correlation findings. Conclusions: This is the first report of HPyV6 detection in the healthy skin of a Brazilian population, but the role of both polyomaviruses in NMSC has yet to be demonstrated. Background HPyV MCC, MCC , (MCC) dermatoses respectively population Objectives Methods diseases 8 patients Results 40 samples 96 9 6 9.6% SCC (SCC BCC (BCC p 0.016 0016 0 016 0.012. 0012 0.012 . 012 0.012) kappa 0.301 0301 301 virus limitations findings Conclusions demonstrated (MCC 4 9.6 0.01 001 01 0.30 030 30 9. 0.0 00 0.3 03 3 0.

Abstract Introduction Nurses from the night shift are exposed to sleep deprivation, which is associated with circadian rhythm alteration, lifestyle changes, psychosocial stress, and, consequently, increased risk of blood pressure (BP) deregulation and hypertension. Objective To analyze risk factors associated with elevated BP levels in nursing workers. Methods A transversal, quantitative study was conducted with 172 nursing professionals of a large hospital in the state of Minas Gerais, Brazil. The following data were collected: anthropometric and BP measurements, sociodemographic characteristics, clinical variables, and lifestyle habits. Results were evaluated by bivariate analysis and logistic regression. The level of significance adopted in the statistical analysis was 5%. Results Participants’ average age was 42.7 ± 9.6 years old; 86.6% (n = 149) were female, and 20.3% (n = 35) had previous diagnosis of hypertension. Overweight and obesity (odds ratio [OR]: 2.187, 95% confidence interval [CI]: 1.060 – 4.509) and night shift (OR: 2.100, CI 95%: 1.061 – 4.158) were statistically significant (p < 0.05) for increased risk of elevated BP level. Conclusion Excessive weight and night shift were significant factors for increased BP in nursing workers. deprivation alteration changes stress consequently (BP hypertension workers transversal 17 Gerais Brazil collected measurements characteristics variables habits regression 5 5% Participants 427 42 7 42. 96 9 6 9. old 866 86 86.6 n 149 female 203 20 3 20.3 35 odds OR [OR] 2187 2 187 2.187 95 [CI] 1060 1 060 1.06 4.509 4509 4 509 (OR 2100 100 2.100 1061 061 4.158 4158 158 p 0.05 005 0 05 8 86. 14 20. [OR 218 18 2.18 [CI 106 06 1.0 4.50 450 50 210 10 2.10 4.15 415 15 0.0 00 21 2.1 1. 4.5 45 4.1 41 0. 2. 4.

RESUMEN Objetivo: Construir y validar escenarios de simulación clínica para la atención de emergencia de pacientes con dolor torácico. Métodos: Estudio metodológico realizado en dos etapas: construcción y validación. La construcción se dio a través del levantamiento de evidencias de la literatura nacional e internacional. La etapa de validación se dio a través de la evaluación de los instrumentos por parte de los jueces, según el Índice de Validación de Contenido y aplicación de la prueba piloto con el público objetivo. Quince jueces con experiencia en simulación, enseñanza y/o asistencia participaron de la investigación, además de 18 estudiantes de enfermería, en la prueba piloto. Resultados: Se construyeron dos escenarios de simulación clínica y todos los ítems evaluados obtuvieron un valor superior a 0,80, mostrando evidencias de validez, siendo considerados instrumentos aptos para su aplicación. Conclusión: La investigación contribuyó al desarrollo y validación de instrumentos que pueden ser aplicados para la enseñanza, evaluación y entrenamiento en simulación clínica en la atención de emergencia a pacientes con dolor torácico. Objetivo torácico Métodos etapas internacional objetivo yo o 1 enfermería Resultados 080 0 80 0,80 validez Conclusión 08 8 0,8 0,

ABSTRACT Objective: To construct and validate clinical simulation scenarios for emergency care for patients with chest pain. Methods: A methodological study carried out in two stages: construction and validity. The construction took place through the survey of evidence from national and international literature. The validity stage took place through instrument assessment by judges, according to the Content Validity Index and application of a pilot test with the target audience. Fifteen judges with expertise in simulation, teaching and/or care participated in the research, in addition to 18 nursing students, in the pilot test. Results: Two scenarios of clinical simulation were constructed, and all the assessed items obtained a value above 0.80, showing evidence of validity, being considered instruments suitable for application. Conclusion: The research contributed to the development and validity of instruments that can be applied for teaching, assessment and training in clinical simulation in emergency care for patients with chest pain. Objective pain Methods stages literature audience andor or 1 students Results constructed 080 0 80 0.80 Conclusion 08 8 0.8 0.

RESUMO Objetivo: Construir e validar cenários de simulação clínica para o atendimento de emergência ao paciente com dor torácica. Métodos: Estudo metodológico realizado em duas etapas: construção e validação. A construção deu-se por meio do levantamento de evidências da literatura nacional e internacional. A etapa de validação deu-se mediante avaliação dos instrumentos pelos juízes, conforme Índice de Validação de Conteúdo e aplicação do teste-piloto com o público-alvo. Participaram da pesquisa 15 juízes com expertise em simulação, docência e/ou assistência, além de18 estudantes de Enfermagem, no teste piloto. Resultados: Foram construídos dois cenários de simulação clínica e todos os itens avaliados obtiveram valor acima de 0,80 apresentando evidência de validade, sendo considerados instrumentos aptos para aplicação. Conclusão: A pesquisa contribuiu para a elaboração e a validação de instrumentos que podem ser aplicados para o ensino, avaliação e capacitação em simulação clínica no atendimento de emergência ao paciente com dor torácica. Objetivo torácica Métodos etapas deuse deu se internacional testepiloto piloto públicoalvo. públicoalvo público alvo. alvo público-alvo 1 eou ou assistência de1 Enfermagem Resultados 080 0 80 0,8 validade Conclusão ensino 08 8 0,

ABSTRACT Background: Women living with human immunodeficiency virus (HIV) (WLWH) are more likely to be infected with the oncogenic human papillomavirus (HPV). We assessed the prevalence of high-risk (HR) (16/18/31/33/35/39/45/51/52/56/58/59/68/73/82), probable high-risk (pHR) (26/53/66), and low-risk (LR) (6/11/40/42/43/44/54/61/70) HPV types and their associated risk factors. Methods: This cross-sectional study of WLWH aged 18-64 years included one laboratory and eight HIV-specialty healthcare facilities in the pilot network. Descriptive statistics were used to assess sociodemographic and behavioral characteristics. Adjusted analyses were conducted to evaluate risk factors associated with HR and/or pHR HPV infection in WLWH. Results: From May/2021 to May/2022, 1,914 (92.5%) WLWH participated in the pilot study and had valid HPV-DNA results of self-collected vaginal samples. The median age of the participants was 45 years, 60.1% had ≥ 9 years of schooling, 80.5% were ≤ 18 years at first sexual intercourse, and 51.7% had > 4 sexual partners throughout life. The prevalence of any HPV type, HR HPV, pHR HPV, and LR HPV was 65.8%, 49.6%, 16.7%, and 40.0%, respectively. Age was inversely associated with pHR and/or HR-HPV (p < 0.001), and education level was inversely associated with HR-HPV (p = 0.003) types. Any HR or pHR was associated with being single (p = 0.029) and exchanging sex for drugs (p = 0.037). Conclusions: The prevalence of HPV, especially HR HPV, among WLWH is high in Brazil, highlighting the need for HPV screening in this population. Self-collection of vaginal samples is an important strategy for increasing testing access. Background HIV (HIV (WLWH HPV. . (HPV) highrisk (HR 16/18/31/33/35/39/45/51/52/56/58/59/68/73/82, 161831333539455152565859687382 16/18/31/33/35/39/45/51/52/56/58/59/68/73/82 , 16 31 33 35 39 51 52 56 58 59 68 73 82 (16/18/31/33/35/39/45/51/52/56/58/59/68/73/82) (pHR 26/53/66, 265366 26/53/66 26 53 66 (26/53/66) lowrisk low (LR 6/11/40/42/43/44/54/61/70 61140424344546170 6 11 40 42 43 44 54 61 70 (6/11/40/42/43/44/54/61/70 Methods crosssectional cross sectional 1864 64 18-6 HIVspecialty specialty network characteristics andor Results May2021 May 2021 May/202 May2022 2022 May/2022 1914 1 914 1,91 92.5% 925 92 5 (92.5% HPVDNA DNA selfcollected self collected 601 60 60.1 schooling 805 80 80.5 intercourse 517 7 51.7 life type 658 65 8 65.8% 496 49 49.6% 167 16.7% 400 0 40.0% respectively HRHPV p 0.001, 0001 0.001 001 0.001) 0.003 0003 003 0.029 0029 029 0.037. 0037 0.037 037 0.037) Conclusions Brazil population Selfcollection Self collection access (HPV 16183133353945515256585968738 16/18/31/33/35/39/45/51/52/56/58/59/68/73/8 3 (16/18/31/33/35/39/45/51/52/56/58/59/68/73/82 26536 26/53/6 2 (26/53/66 6/11/40/42/43/44/54/61/7 6114042434454617 (6/11/40/42/43/44/54/61/7 186 18- May202 202 May/20 191 91 1,9 92.5 (92.5 60. 80. 51. 65.8 49.6 16.7 40.0 000 0.00 00 0.02 002 02 0.03 03 1618313335394551525658596873 16/18/31/33/35/39/45/51/52/56/58/59/68/73/ (16/18/31/33/35/39/45/51/52/56/58/59/68/73/8 2653 26/53/ (26/53/6 6/11/40/42/43/44/54/61/ 611404243445461 (6/11/40/42/43/44/54/61/ May20 20 May/2 19 1, 92. (92. 65. 49. 16. 40. 0.0 161831333539455152565859687 16/18/31/33/35/39/45/51/52/56/58/59/68/73 (16/18/31/33/35/39/45/51/52/56/58/59/68/73/ 265 26/53 (26/53/ 6/11/40/42/43/44/54/61 61140424344546 (6/11/40/42/43/44/54/61 May2 May/ (92 0. 16183133353945515256585968 16/18/31/33/35/39/45/51/52/56/58/59/68/7 (16/18/31/33/35/39/45/51/52/56/58/59/68/73 26/5 (26/53 6/11/40/42/43/44/54/6 6114042434454 (6/11/40/42/43/44/54/6 (9 1618313335394551525658596 16/18/31/33/35/39/45/51/52/56/58/59/68/ (16/18/31/33/35/39/45/51/52/56/58/59/68/7 26/ (26/5 6/11/40/42/43/44/54/ 611404243445 (6/11/40/42/43/44/54/ ( 161831333539455152565859 16/18/31/33/35/39/45/51/52/56/58/59/68 (16/18/31/33/35/39/45/51/52/56/58/59/68/ (26/ 6/11/40/42/43/44/54 61140424344 (6/11/40/42/43/44/54 16183133353945515256585 16/18/31/33/35/39/45/51/52/56/58/59/6 (16/18/31/33/35/39/45/51/52/56/58/59/68 (26 6/11/40/42/43/44/5 6114042434 (6/11/40/42/43/44/5 1618313335394551525658 16/18/31/33/35/39/45/51/52/56/58/59/ (16/18/31/33/35/39/45/51/52/56/58/59/6 (2 6/11/40/42/43/44/ 611404243 (6/11/40/42/43/44/ 161831333539455152565 16/18/31/33/35/39/45/51/52/56/58/59 (16/18/31/33/35/39/45/51/52/56/58/59/ 6/11/40/42/43/44 61140424 (6/11/40/42/43/44 16183133353945515256 16/18/31/33/35/39/45/51/52/56/58/5 (16/18/31/33/35/39/45/51/52/56/58/59 6/11/40/42/43/4 6114042 (6/11/40/42/43/4 1618313335394551525 16/18/31/33/35/39/45/51/52/56/58/ (16/18/31/33/35/39/45/51/52/56/58/5 6/11/40/42/43/ 611404 (6/11/40/42/43/ 161831333539455152 16/18/31/33/35/39/45/51/52/56/58 (16/18/31/33/35/39/45/51/52/56/58/ 6/11/40/42/43 61140 (6/11/40/42/43 16183133353945515 16/18/31/33/35/39/45/51/52/56/5 (16/18/31/33/35/39/45/51/52/56/58 6/11/40/42/4 6114 (6/11/40/42/4 1618313335394551 16/18/31/33/35/39/45/51/52/56/ (16/18/31/33/35/39/45/51/52/56/5 6/11/40/42/ 611 (6/11/40/42/ 161831333539455 16/18/31/33/35/39/45/51/52/56 (16/18/31/33/35/39/45/51/52/56/ 6/11/40/42 (6/11/40/42 16183133353945 16/18/31/33/35/39/45/51/52/5 (16/18/31/33/35/39/45/51/52/56 6/11/40/4 (6/11/40/4 1618313335394 16/18/31/33/35/39/45/51/52/ (16/18/31/33/35/39/45/51/52/5 6/11/40/ (6/11/40/ 161831333539 16/18/31/33/35/39/45/51/52 (16/18/31/33/35/39/45/51/52/ 6/11/40 (6/11/40 16183133353 16/18/31/33/35/39/45/51/5 (16/18/31/33/35/39/45/51/52 6/11/4 (6/11/4 1618313335 16/18/31/33/35/39/45/51/ (16/18/31/33/35/39/45/51/5 6/11/ (6/11/ 161831333 16/18/31/33/35/39/45/51 (16/18/31/33/35/39/45/51/ 6/11 (6/11 16183133 16/18/31/33/35/39/45/5 (16/18/31/33/35/39/45/51 6/1 (6/1 1618313 16/18/31/33/35/39/45/ (16/18/31/33/35/39/45/5 6/ (6/ 161831 16/18/31/33/35/39/45 (16/18/31/33/35/39/45/ (6 16183 16/18/31/33/35/39/4 (16/18/31/33/35/39/45 1618 16/18/31/33/35/39/ (16/18/31/33/35/39/4 161 16/18/31/33/35/39 (16/18/31/33/35/39/ 16/18/31/33/35/3 (16/18/31/33/35/39 16/18/31/33/35/ (16/18/31/33/35/3 16/18/31/33/35 (16/18/31/33/35/ 16/18/31/33/3 (16/18/31/33/35 16/18/31/33/ (16/18/31/33/3 16/18/31/33 (16/18/31/33/ 16/18/31/3 (16/18/31/33 16/18/31/ (16/18/31/3 16/18/31 (16/18/31/ 16/18/3 (16/18/31 16/18/ (16/18/3 16/18 (16/18/ 16/1 (16/18 16/ (16/1 (16/ (16 (1

Abstract Background Despite no evidence showing benefits of hydroxychloroquine and chloroquine with or without azithromycin for COVID-19 treatment, these medications have been largely prescribed in Brazil. Objectives To assess outcomes, including in-hospital mortality, electrocardiographic abnormalities, hospital length-of-stay, admission to the intensive care unit, and need for dialysis and mechanical ventilation, in hospitalized COVID-19 patients who received chloroquine or hydroxychloroquine, and to compare outcomes between those patients and their matched controls. Methods A retrospective multicenter cohort study that included consecutive laboratory-confirmed COVID-19 patients from 37 Brazilian hospitals from March to September 2020. Propensity score was used to select matching controls by age, sex, cardiovascular comorbidities, and in-hospital use of corticosteroid. A p-value <0.05 was considered statistically significant. Results From 7,850 COVID-19 patients, 673 (8.6%) received hydroxychloroquine and 67 (0.9%) chloroquine. The median age in the study group was 60 years (46 - 71) and 59.1% were women. During hospitalization, 3.2% of patients presented side effects and 2.2% required therapy discontinuation. Electrocardiographic abnormalities were more prevalent in the chloroquine/hydroxychloroquine group (13.2% vs. 8.2%, p=0.01), and the long corrected QT interval was the main difference (3.6% vs. 0.4%, p<0.001). The median hospital length of stay was longer in the HCQ/CQ + AZT group than in controls (9.0 [5.0, 18.0] vs. 8.0 [4.0, 14.0] days). There was no statistical differences between groups in intensive care unit admission (35.1% vs. 32.0%; p=0.282), invasive mechanical ventilation support (27.0% vs. 22.3%; p=0.074) or mortality (18.9% vs. 18.0%; p=0.682). Conclusion COVID-19 patients treated with chloroquine or hydroxychloroquine had a longer hospital length of stay, when compared to matched controls. Intensive care unit admission, invasive mechanical ventilation, dialysis and in-hospital mortality were similar. COVID19 COVID 19 COVID-1 treatment Brazil inhospital lengthofstay, lengthofstay length-of-stay laboratoryconfirmed laboratory confirmed 3 2020 sex comorbidities corticosteroid pvalue p value 005 0 05 <0.0 significant 7850 7 850 7,85 8.6% 86 8 6 (8.6% 0.9% 09 9 (0.9% 46 (4 71 591 59 1 59.1 women hospitalization 32 2 3.2 22 2.2 discontinuation chloroquinehydroxychloroquine 13.2% 132 13 (13.2 vs 82 8.2% p=0.01, p001 p=0.01 , 01 p=0.01) 3.6% 36 (3.6 04 4 0.4% p<0.001. p0001 p<0.001 . 001 p<0.001) HCQCQ HCQ CQ 9.0 90 (9. 5.0, 50 5 [5.0 18.0 180 18 80 8. 4.0, 40 [4.0 14.0 140 14 days. days days) 35.1% 351 35 (35.1 32.0% 320 p=0.282, p0282 p=0.282 282 p=0.282) 27.0% 270 27 (27.0 22.3% 223 p=0.074 p0074 074 18.9% 189 (18.9 18.0% p=0.682. p0682 p=0.682 682 p=0.682) similar COVID1 COVID- 202 00 <0. 785 85 7,8 8.6 (8.6 0.9 (0.9 ( 59. 3. 2. 13.2 (13. 8.2 p00 p=0.0 3.6 (3. 0.4 p000 p<0.00 9. (9 5.0 [5. 18. 4.0 [4. 14. 35.1 (35. 32.0 p028 p=0.28 28 27.0 (27. 22.3 p=0.07 p007 07 18.9 (18. p068 p=0.68 68 20 <0 78 7, (8. 0. (0. 13. (13 p0 p=0. (3 p<0.0 5. [5 4. [4 35. (35 32. p02 p=0.2 27. (27 22. (18 p06 p=0.6 < (8 (0 (1 p=0 p<0. [ (2 p= p<0 p<

Resumo Fundamento Apesar da ausência de evidência mostrando benefícios da hidroxicloroquina e da cloroquina combinadas ou não à azitromicina no tratamento da covid-19, esses medicamentos têm sido amplamente prescritos no Brasil. Objetivos Avaliar desfechos, incluindo moralidade hospitalar, alterações eletrocardiográficas, tempo de internação, admissão na unidade de terapia intensiva, e necessidade de diálise e de ventilação mecânica em pacientes hospitalizados com covid-19 que receberam cloroquina ou hidroxicloroquina, e comparar os desfechos entre aqueles pacientes e seus controles pareados. Métodos Estudo multicêntrico retrospectivo do tipo coorte que incluiu pacientes com diagnóstico laboratorial de covid-19 de 37 hospitais no Brasil de março a setembro de 2020. Escore de propensão foi usado para selecionar controles pareados quanto a idade, sexo, comorbidades cardiovasculares, e uso de corticosteroides durante a internação. Um valor de p<0,05 foi considerado estatisticamente significativo. Resultados Dos 7850 pacientes com covid-19, 673 (8,6%) receberam hidroxicloroquina e 67 (0,9%) cloroquina. A idade mediana no grupo de estudo foi 60 (46-71) anos e 59,1% eram mulheres. Durante a internação, 3,2% dos pacientes apresentaram efeitos adversos e 2,2% necessitaram de interromper o tratamento. Alterações eletrocardiográficas foram mais prevalentes no grupo hidroxicloroquina/cloroquina (13,2% vs. 8,2%, p=0,01), e o prolongamento do intervalo QT corrigido foi a principal diferença (3,6% vs. 0,4%, p<0,001). O tempo mediano de internação hospitalar foi maior no grupo usando CQ/HCQ em relação aos controles (9,0 [5,0-18,0] vs. 8,0 [4,0-14,0] dias). Não houve diferenças estatisticamente significativas entre os grupos quanto a admissão na unidade de terapia intensiva (35,1% vs. 32,0%; p=0,282), ventilação mecânica invasiva (27,0% vs. 22,3%; p=0,074) ou mortalidade (18,9% vs. 18,0%; p=0,682). Conclusão Pacientes com covid-19 tratados com cloroquina ou hidroxicloroquina apresentaram maior tempo de internação hospitalar, em comparação aos controles. Não houve diferença em relação a admissão em unidade de terapia intensiva, necessidade de ventilação mecânica e mortalidade hospitalar. covid19, covid19 covid 19, 19 covid-1 3 2020 sexo cardiovasculares p005 p 0 05 p<0,0 significativo 785 8,6% 86 8 6 (8,6% 0,9% 09 9 (0,9% 4671 46 71 (46-71 591 59 1 59,1 mulheres 32 2 3,2 22 2,2 hidroxicloroquinacloroquina 13,2% 132 13 (13,2 vs 82 8,2% p=0,01, p001 p=0,01 , 01 p=0,01) 3,6% 36 (3,6 04 4 0,4% p<0,001. p0001 p<0,001 . 001 p<0,001) CQHCQ CQ HCQ 9,0 90 (9, 5,018,0 50180 5,0 18,0 5 18 [5,0-18,0 80 8, 4,014,0 40140 4,0 14,0 14 [4,0-14,0 dias. dias dias) 35,1% 351 35 (35,1 32,0% 320 p=0,282, p0282 p=0,282 282 p=0,282) 27,0% 270 27 (27,0 22,3% 223 p=0,074 p0074 074 18,9% 189 (18,9 18,0% 180 p=0,682. p0682 p=0,682 682 p=0,682) covid1 covid- 202 p00 p<0, 78 8,6 (8,6 0,9 (0,9 467 7 (46-7 59, 3, 2, 13,2 (13, 8,2 p=0,0 3,6 (3, 0,4 p000 p<0,00 00 9, (9 018 5,018, 5018 50 5, 18, [5,0-18, 014 4,014, 4014 40 4, 140 14, [4,0-14, 35,1 (35, 32,0 p028 p=0,28 28 27,0 (27, 22,3 p=0,07 p007 07 18,9 (18, p068 p=0,68 68 20 p0 p<0 (8, 0, (0, (46- 13, (13 p=0, (3 ( 5,018 501 [5,0-18 4,014 401 [4,0-14 35, (35 32, p02 p=0,2 27, (27 22, (18 p06 p=0,6 p< (8 (0 (46 (1 p=0 5,01 [5,0-1 4,01 [4,0-1 (2 (4 p= [5,0- [4,0- [5,0 [4,0 [5, [4, [5 [4 [

Abstract Background: Risk stratification on admission of patients with acute ST-elevation myocardial infarction (STEMI) is considered a clear strategy for effective treatment, early intervention, and survival. Objective: The purpose of this study was to determine the risk factors for in-hospital mortality from cardiac causes after STEMI. Methods: Observational, retrospective, longitudinal study, with a quantitative approach, based on data from the medical records of individuals diagnosed with STEMI treated at the Emergency Room of a large hospital in the state of Minas Gerais, Brazil, from January 2011 to July 2016. The outcome of interest was 30-day in-hospital mortality from after STEMI. For statistical analysis, the Pearson's chi-square test, Spearman's correlation and multivariable Cox-regression analysis were used, with a significance level of α = 0.05. Results: Of the 459 patients, 55 (12%) died from cardiac causes within 30 days after STEMI. Mean admission SBP of these patients was 109.08mmHg. The incidence of death was higher in women (23.7%), patients with systemic arterial hypertension (SAH) (13.8%) and elderly patients (16.5%). The elderly — heart rate (HR) = 3.54 — and women — HR = 2.55 — had a statistically significant higher risk of progressing to death when compared to younger adults and men. The highest admission SBP had a protective effect (HR = 0.97), reducing the chance of death by 3%. Conclusion: SBP on admission, female gender and advanced age were significant risk factors for death within 30 days after STEMI. Background STelevation ST elevation (STEMI treatment intervention survival Objective inhospital Methods Observational retrospective approach Gerais Brazil 201 2016 30day day Pearsons Pearson s chisquare chi square test Spearmans Spearman Coxregression Cox regression used 005 0 05 0.05 Results 45 5 12% 12 (12% 3 10908mmHg mmHg 109 08mmHg 109.08mmHg 23.7%, 237 23.7% , 23 7 (23.7%) SAH (SAH 13.8% 138 13 8 (13.8% 16.5%. 165 16.5% . 16 (16.5%) 354 54 3.5 255 2 2.5 men 0.97, 097 0.97 97 0.97) 3% Conclusion 20 00 0.0 4 1 (12 10 23.7 (23.7% 13.8 (13.8 16.5 (16.5% 35 3. 25 2. 09 0.9 9 0. (1 23. (23.7 13. (13. 16. (16.5 ( (23. (13 (16. (23 (16 (2

ABSTRACT Dialysis-associated immune dysfunction makes chronic kidney disease patients both susceptible to severe Coronavirus disease 19 (Covid19) and to a weaker response to vaccination. Previously infected patients are thought to sustain stronger and more durable humoral responses than vaccinated patients. Four months after a two dose-regimen of the Pfizer/BioNTech SARS-CoV-2 mRNA vaccine (BNT162b2), we evaluated the SARS-CoV-2 spike immunoglobin G (IgG-S1) antibody levels in previously infected peritoneal dialysis patients and compared them with infection naïve PD patients. A total of 79 peritoneal dialysis patients were analyzed, of which 11 had a previous history of Covid19. We have verified that the median titer of the IgG-S1 in previously infected patients (14310 AU/mL) was significantly superior to that in infection naïve patients (760,05 AU/mL) (p < 0,001). Previous Covid19 was the only significant predictor of IgG-S1 levels in a multivariate linear regression model (p < 0,001). These results may impact vaccination strategies for peritoneal dialysis patients regarding the future administration of BNT162b2 booster doses. In conclusion, previously infected peritoneal dialysis patients who have completed a two-dose regimen of the BNT162b2 may be well suited without a third, booster dose for longer than infection naïve peritoneal dialysis patients. This strategy could make additional doses available around the world.

Abstract We present here the first record of Salacia tetracythara as an alien species for the Atlantic Ocean, specifically the coast of the state of Sergipe, northeast Brazil. The species was found attached to an artificial experimental plate installed in the rainy period (July-October 2017), in an estuarine region of the Sergipe River near the Sergipe Harbour. Salacia tetracythara is characterized by a small triangular space below each hydrotheca and two types of microbasic mastigophore nematocysts that differ in shape, size, and location. The species was described from Australia and has been recorded only for tropical to temperate regions of the Indo-Pacific. Salacia tetracythara seems to be a recent introduction into the Atlantic Ocean and, its introduction probably derived from ship transport between the Sergipe Harbour and regions of Oceania and/or Asia. Further investigation should be conducted to determine whether the species is established in the region and how far inside the estuary it has gone.

Abstract The new table grape cultivar BRS Tainá produces white firm seedless grapes, agreeable flavor, balanced sugar-acid ratio, good berry adherence to the pedicel and yield of 50 ton ha-1 year-1. It is recommended for the tropical conditions of the Submédio do Vale do São Francisco. Propagative material is acquired from nurseries licensed by Embrapa.

Abstract To assess the effect of vertical sleeve gastrectomy (VSG) and Roux-en-Y gastric bypass (RYGB) on the esophageal and intestinal morphology of western diet (WD)-obese rats and to characterize the stomach histopathology of WD rats submitted to VSG. Male Wistar rats received WD from 2-4 months of age, to induce obesity, before randomly submitting them to pseudo (WD-SHAM), VSG (WD-VSG) or RYGB (WD-RYGB) surgeries. Gastrointestinal histomorphometry was performed at 3-months post-surgery. The upper esophagus of VSG and RYGB rats increased luminal area, while reductions in the keratin layer of the mucosa and the tunica muscularis were observed only in the RYGB animals. In the lower esophagus, both surgeries increased keratin layer thickness, but reduced the mucosal mucus content, while RYGB increased the thickness of the tunica mucosa and muscularis. The glandular region of the stomach of WD-VSG rats exhibited hypotrophy, epithelial erosion, fibrosis and moderate inflammatory infiltration. VSG and RYGB increased the villi height in the ileum, and the thickness of the tunica muscularis in the jejunum and ileum of WD rats; furthermore, RYGB augmented the ileal villi height. Thus both approaches induced histomorphological alterations in the esophagus and intestine and VSG damaged the gastric mucosa, even over the long-term.

RESUMO Objetivo: Analisar a influência da neuropatia diabética periférica (NDP) no equilíbrio postural, atividades funcionais e na qualidade de vida em idosos. Métodos: Estudo transversal. Avaliamos 60 homens e mulheres idosos (60-79 anos) divididos em três grupos: controle, DM sem e com NDP. Foram avaliados: antropometria; qualidade de vida; equilíbrio postural (BESTest); atividades funcionais pelo equilíbrio funcional na placa de força (NeuroCom Balance). Resultados: Grupo NDP apresentou diferenças comparado a outros grupos, pior desempenho na qualidade de vida que o DM2 sem NDP em: funcionamento sensorial (p = 0,030); passado e futuro (p = 0,036); morte e morrer (p = 0,035). Déficit de equilíbrio postural no escore total (p = 0,025) e seção de restrições biomédicas (p = 0,043) do BESTest comparado ao DM2 sem NDP (p = 0,007). No equilíbrio funcional (Neurocom), o grupo NDP apresentou pior desempenho no tempo gasto no lado esquerdo (p = 0,030) comparado ao grupo controle. Durante a etapa de teste, o grupo controle executou a tarefa mais rapidamente que o grupo NDP (p = 0,004). Conclusão: Neuropatas apresentaram pior desempenho físico e déficits no equilíbrio postural, limitações sensoriais, afetando as tarefas diárias da doença e, consequentemente, diminuição da qualidade de vida em idosos brasileiros. Nível de Evidência II, Estudo observacional transversal.

ABSTRACT Objective: To analyze the impact of the Peripheral Diabetic Neuropathy (PDN) on the postural and functional balance and quality of life of Brazilian older adults. Methods: A cross-sectional study. Sixty older men and women (60-79 years) were divided into three groups: control, DM without and with PDN. The following parameters were evaluated: anthropometry; quality of life; postural balance (BESTest); functional balance in force plate (NeuroCom Balance). Results: PDN group presented significant differences compared with the other groups, with the worst performance in quality of life than DM2 without PDN in: sensory functioning (p = 0.030); past and future (p = 0.036); death and dying (p = 0.035). Postural balance deficit in the total score (p = 0.025) and biomedical constraints section (p = 0.043) of the BESTest, compared with DM2 without PDN (p = 0.007). In the functional balance (Neurocom), PDN group presented a worse performance in the time spent on the left side (p = 0.030) than the control group. During step up over test, the control group performed the task faster than the group with PDN (p = 0.004). Conclusion: This study showed that neuropaths presented worse physical performance and postural balance deficits, sensorial limitations, affecting the daily tasks and, as a consequence, decreasing the quality of life in Brazilian older adults. Level of Evidence II, Cross-sectional observational study.