Abstract This study aimed to resveratrol supplementation (at 5 or 10 mg/kg) and a hydroethanolic extract of canjiqueira fruits (150 mg/kg) on female SWISS mice. Total cholesterol, high-density lipoprotein (HDL), triglyceride levels, gestation rates, and embryonic implantation rates in their female Offspring was evaluated. In conclusion, the consumption of canjiqueira fruit extract altered the lipid profile of their female offspring, and did not impact their reproductive performance. Supplementing female SWISS mice with 10 mg/kg of resveratrol increased total cholesterol, triglycerides, and HDL levels, thereby enhancing the reproductive efficiency of their offspring. at 1 mgkg mg kg 150 (15 cholesterol highdensity high density HDL, , (HDL) levels evaluated conclusion offspring performance triglycerides 15 (1 (HDL (

Resumo O objetivo deste trabalho foi avaliar o desempenho agronômico de milho (Zea mays) solteiro e consorciado com Urochloa ruziziensis em sucessão à soja (Glycine max) inoculada com Bradyrhizobium japonicum, bem com a inoculação e a reinoculação do milho com Azospirillum brasilense, em dois solos contrastantes. O experimento foi realizado em duas safras, em delineamento de blocos ao acaso, em arranjo fatorial 2x2x3, com cinco repetições. O primeiro fator avaliado foi composto pelos dois solos contrastantes: Latossolo Vermelho eutrófico argiloso e Latossolo Vermelho distrófico arenoso. O segundo fator foi o cultivo de milho: solteiro e consorciado com U. ruziziensis. Já o terceiro fator consistiu na inoculação em soja e milho em sucessão, como a seguir: sem inoculação nos dois cultivos, inoculação com Bradyrhizobium japonicum em soja e com A. brasilense em milho, e reinoculação com B. japonicum + A. brasilense em soja e com A. brasilense em milho. Nos dois anos de cultivo, as maiores produtividades de milho são observadas no solo argiloso com sementes reinoculadas com A. brasilense. A reinoculação anual com A. brasilense resulta em maior produtividade de grãos de milho no consórcio com U. ruziziensis no solo argiloso. A inoculação e a reinoculação reduzem as perdas de produtividade do milho causadas pela competição com U. ruziziensis no consórcio.

Abstract The objective of this work was to evaluate the agronomic performance of corn (Zea mays) grown single and intercropped with Urochloa ruziziensis in succession to soybean (Glycine max) inoculated with Bradyrhizobium japonicum, as well as the inoculation and reinoculation of corn with Azospirillum brasilense, in two contrasting soils. The experiment was carried out in two crop years, in a randomized complete block design, in a 2x2x3 factorial arrangement, with five replicates. The first evalauted factor was composed of the two constrasting soils: a clayey Rhodic Eutrustox and a sandy Rhodic Haplustox. The second factor was corn cultivation: single and intercropped with U. ruziziensis. The third factor consisted of the inoculation onto soybean and corn in succession, as follows: no inoculation in both crops, inoculation with B. japonicum onto soybean and with A. brasilense onto corn, and reinoculation with B. japonicum + A. brasilense onto soybean and with A. brasilense onto corn. In both crop years, the highest corn yields are observed in clayey soil with seeds reinoculated with A. brasilense. The annual reinoculation with A. brasilense results in a higher corn grain yield in the intercropping with U. ruziziensis in the clayey soil. Inoculation and reinoculation reduce corn yield losses caused by the competition with U. ruziziensis in intercropping.

Abstract: Several microbiological indicators of soil quality present high sensitivity, but little is known about the influence of topographic factors on them. This work aimed to evaluate variability of biological indicators of soil quality across a hillslope under native forest and the influence of topographic factors on them. Four positions on a hillslope were evaluated. Activity of the enzymes β-glucosidase, acid phosphatase, urease and fluorescein diacetate (FDA) hydrolysis were determined, as well as basal and substrate-induced respiration, and density of microorganisms: total bacteria, total fungi, actinobacteria, phosphate solubilizers, ammonifiers, native rhizobia, free-living N2-fixing bacteria, spores of arbuscular mycorrhizal fungi and percentage of root colonization by arbuscular mycorrhizal fungi. Activity and density of microorganisms were correlated with topographic factors. The relation of these factors to the variations of the evaluated indicators was determined using the random forest algorithm. Microbiological indicators varied according to the hillslope positions. The indicators urease, basal respiration, spore density, mycorrhizal colonization, total bacteria and fungi, phosphate solubilizers, and free-living N2-fixing bacteria detected in JNFB and FAM culture medium did not vary with terrain attributes and were therefore more indicated in cases of topographic variations. This and future studies can help to select the best microbiological indicators for different conditions.

Resumo: O objetivo deste trabalho foi avaliar o perfil fitoquímico e a bioatividade de extratos da urtiga-cansanção (Cnidoscolus urens) sobre a lagarta-da-couve (Ascia monuste orseis). Foram avaliadas a mortalidade, duração da fase larval, viabilidade e massa de pupas, percentagem de pupas deformadas e preferência alimentar. Também foram identificadas as classes constituintes dos extratos etanólicos, por meio de cromatografia em camada delgada analítica (CCDA). Os extratos etanólicos de folhas e raízes a 2% (m/v) e o extrato aquoso de raízes a 20% (m/v) prolongaram a fase larval do inseto. O extrato etanólico de folhas a 2% reduziu a viabilidade pupal, e as pupas provenientes de todos os tratamentos apresentaram massas reduzidas e geraram adultos defeituosos. Com exceção do extrato etanólico de caule a 2% (m/v), todos os demais extratos apresentaram efeito deterrente sobre larvas de A. monuste orseis. A análise fitoquímica dos extratos etanólicos mostrou a presença majoritária de compostos fenólicos, naftoquinonas, antraquinonas, cumarinas, derivados antracênicos, terpenos e esteroides, além de taninos hidrossolúveis. Extratos aquosos à concentração de 20% e etanólicos a 2% de folhas, caules e raízes de urtiga-cansanção causam mortalidade larval e também reduzem o consumo foliar, a viabilidade pupal e a massa de pupas de A. monuste orseis.

Abstract: The objective of this work was to evaluate the phytochemical profile and bioactivity of extracts of Brazilian stinging (“cansanção”) nettle (Cnidoscolus urens) on cabbage caterpillar (Ascia monuste orseis). Mortality, larval stage duration, pupal viability and mass, percentage of deformed pupae, and feeding preference were evaluated. The constituent classes of the ethanolic extracts were also identified by analytical thin-layer chromatography (TLC). Ethanolic extracts of leaves and roots at 2% (m/v) and aqueous extract of roots at 20% (m/v) prolonged the larval phase of the insect. The ethanolic extract of leaves at 2% reduced pupal viability, and pupae from all treatments showed reduced masses and generated deformed adults. Except for the ethanolic extract of stem at 2% (m/v), all other extracts showed a deterrent effect on A. monuste orseis larvae. The phytochemical analysis of ethanolic extracts showed the major presence of phenolic compounds, naphthoquinones, anthraquinones, coumarins, anthracene derivatives, terpenes, and steroids, as well as tannins. Aqueous extracts at 20% concentration and ethanolic extracts at 2% of leaves, stems, and roots of cansanção nettle cause larval mortality and also reduce leaf consumption, pupal viability, and pupal mass of A. monuste orseis.

RESUMO O beneficiamento dos minérios de ouro explotados em Paracatu, MG, gera rejeitos com características limitantes à revegetação, principalmente pelo elevado teor de arsênio e salinidade. Visando à revegetação da área de depósito desses rejeitos, o objetivo deste trabalho foi avaliar os efeitos de doses de fósforo sobre plantas de vetiver (Chrysopogon zizanioides) e sobre o substrato de plantio, que consistiu em um rejeito proveniente do beneficiamento do minério denominado B1, explotado em Paracatu, com restrição hídrica. O vetiver foi cultivado durante quatro meses no substrato sob as doses de 0, 140, 280, 560 e 1280 mg kg-1 de P2O5. Com o aumento das doses de fósforo houve melhoria nas características químicas do substrato, porém a dose mais elevada (1280 mg kg-1 de P2O5) propiciou maior toxicidade de arsênio nas plantas. Nas condições avaliadas, a dose de 560 mg kg-1 de P2O5 é a mais adequada para a adubação do vetiver, visando à revegetação do substrato. O vetiver sobrevive à baixa disponibilidade de água no rejeito.

ABSTRACT The processing of gold ores exploited in Paracatu, MG, generates tailings that are challenging for revegetation, mainly because of the high content of arsenic and salinity. Aiming at the revegetation of the area of disposal of these tailings, the objective of this study was to evaluate the effects of phosphorus doses on vetiver plants (Chrysopogon zizanioides) and on the planting substrate, which consisted of tailings from the processing of ore named B1, exploited in Paracatu, with water restriction. Vetiver was grown for four months in the substrate under doses of 0, 140, 280, 560 and 1280 mg kg-1 of P2O5. Increasing doses of phosphorus improved the chemical characteristics of the substrate. However, the highest dose (1280 mg kg-1 P2O5) resulted in higher toxicity of arsenic for the plants. Under the evaluated conditions, the dose of 560 mg kg-1 of P2O5 is the most suitable for the fertilization of vetiver and, therefore, also for the revegetation of the substrate. Vetiver survives under low availability of water in the tailings.

Specimens of Leonotis nepetiflolia were obtained from cultivated and wild environments to verify their influences in chemical composition. Phytochemical analyses were conducted for the ethyl acetate phase obtained by partitioning the crude ethanol extract from the cultivated leaves of L. nepetifolia. In doing so, flavonoid 3',4',5-trimethoxy-6,7-dihidroxyflavone (cirsiliol), a chemotaxonomic marker of the family Lamiaceae, was isolated. The results reveal that all phases and extracts tested exhibited weak to moderate antimicrobial activity against the strains of bacteria tested. The evaluation of in vitrocytotoxic and antitumor activity showed that the ethyl acetate phases obtained from both wild and cultivated leaves exhibit high potential cytotoxic and antitumor (> 78.0% of inhibition) activity. The major component of these phases was identified by high-performance liquid chromatography-diode array detector and nuclear magnetic resonance analyses using both 1D and 2D methods. The results further indicate that the flavonoid cirsiliol is the agent responsible for the activity observed in these phases.

INTRODUÇÃO: A embolização de fragmentos de ateroma/trombo durante a intervenção coronária percutânea (ICP) ocasiona distúrbios de perfusão da microcirculação. O novo stent MGuardTM, que é revestido com uma rede de polietileno, demonstrou eficácia na prevenção de complicações embólicas durante a ICP primária. No entanto, a evolução clínica tardia de pacientes tratados com o stent MGuardTM permanece desconhecida. MÉTODOS: Uma série de 65 pacientes portadores de lesões coronárias de novo tratados com o stent MGuardTM foi analisada. Os dados clínicos basais, do procedimento e do seguimento clínico tardio (média de tempo, 2,6 ± 1,4 anos) foram coletados retrospectivamente por meio da revisão de prontuários médicos e/ou contato telefônico direto. RESULTADOS: A média de idade foi de 66,1 ± 13,7 anos, 32,3% eram diabéticos, 49,2% tinham infarto agudo do miocárdio (IAM) prévio, e 44,6% apresentaram-se com síndrome coronária aguda. Dois terços das lesões estavam localizados em pontes de safena, quase metade tinha presença de trombo e a maioria foi classificada como tipo B2/C. O stent MGuardTM foi implantado com sucesso em todos os casos. Ao final do procedimento, fluxo TIMI 3 foi alcançado em 93,4% e o sucesso angiográfico foi de 91,8%. No seguimento tardio, as taxas de eventos adversos incluíram óbito cardiovascular em 6,2%, IAM não-fatal em 9,2%, revascularização da lesão-alvo em 9,2% e trombose de stent definitiva/provável em 1,5%. CONCLUSÕES: O seguimento tardio de pacientes com lesões coronárias complexas tratados com o stent MGuardTM demonstrou baixas taxas de revascularização da lesão-alvo e de trombose do stent.

BACKGROUND: The embolization of atheroma/thrombus fragments during percutaneous coronary intervention (PCI) causes microcirculatory perfusion disturbances. The new MGuardTM, a mesh-based bare-metal stent, demonstrated efficacy in the prevention of embolic complications during primary PCI. However, the late clinical outcome of patients treated with the MGuardTM stent remains unknown. METHODS: A series of 65 patients with de novo coronary lesions treated with MGuardTM stent was analyzed. Baseline clinical data, procedure and late clinical follow-up (mean duration, 2.6 ± 1.4 years) data were collected retrospectively by a review of medical records and/or direct telephone contact. RESULTS: Mean age was 66.1 ± 13.7 years, 32.3% of patients were diabetic, 49.2% had a previous acute myocardial infarction (AMI), and 44.6% presented with acute coronary syndrome. Two thirds of the lesions were located in a saphenous vein graft, almost half of the lesions had thrombus and most were classified as type B2/C. The MGuardTM stent was successfully implanted in all cases. At the end of the procedure, TIMI 3 flow was achieved in 93.4% and angiographic success was 91.8%. In the late clinical follow-up, adverse event rates included cardiac death in 6.2%, non-fatal AMI in 9.2%, target lesion revascularization in 9.2% and definite/probable stent thrombosis in 1.5%. CONCLUSIONS: The late follow-up of patients with complex coronary lesions treated with the MGuardTM stent demonstrated low rates of target lesion revascularization and stent thrombosis.