ABSTRACT Objective: To measure levels of perceived stress, associated factors, especially the impact of choice and satisfaction with the course, as well as the possible consequences of this outcome among undergraduates. Methods: Cross-sectional study conducted in 2019 with 996 undergraduate students from a public university in southern Brazil. Sampling was carried out systematically by single-stage clusters. The outcome was assessed using the Perceived Stress Scale. Analysis of associated factors and possible consequences were performed using multiple linear regression and Poisson regression, respectively. Results: Mean perceived stress score was 31.1 (SD = 9). Female individuals, with non-heterosexual orientation, poorer, with food insecurity, with fear of violence in the neighborhood, with perception of difficult access to psychological services and with less social support were the most stressed. Older participants with some level of physical activity were less stressed. Dissatisfaction with the current course, rather than the fact that it was the desired course prior admission, was associated with high levels of stress. Non-medical use of prescribed medication, generalized anxiety, depressive symptoms, and poorer quality of sleep and life were identified as possible consequences of stress. Conclusions: The ENEM/SiSU model seems to have no effect on stress. Furthermore, this outcome was higher among more vulnerable individuals, having contributed to several negative outcomes in mental health. It is recommended to strengthen student assistance services so that these cases can be identified and managed. Objective undergraduates Methods Crosssectional Cross sectional 201 99 Brazil singlestage single stage clusters Scale respectively Results 311 31 1 31. SD 9. 9 . 9) individuals nonheterosexual non heterosexual orientation insecurity neighborhood stressed admission Nonmedical Non medical medication anxiety symptoms Conclusions ENEMSiSU ENEM SiSU Furthermore health managed 20 3 2

RESUMO Objetivo: Medir os níveis de estresse percebido, os fatores associados, em especial o impacto da escolha e satisfação com o curso, bem como as possíveis consequências desse desfecho entre graduandos. Métodos: Estudo transversal conduzido em 2019 com 996 estudantes de graduação de uma universidade pública no Sul do Brasil. A amostragem foi realizada de forma sistemática por conglomerados em único estágio. O desfecho foi avaliado por meio da Escala de Estresse Percebido. As análises de fatores associados e possíveis consequências foram realizadas por meio de regressão linear múltipla e regressão de Poisson, respectivamente. Resultados: O escore médio de estresse percebido foi de 31,1 (DP = 9). Indivíduos do sexo feminino, de orientação sexual não heterossexual, mais pobres, com insegurança alimentar, com medo de violência no bairro, com percepção de difícil acesso a serviço psicológico e com menor suporte social foram os mais estressados. Participantes mais velhos e com algum nível de atividade física foram menos estressados. A insatisfação com o curso atual, e não o fato de ser o curso desejado no ingresso, foi associada ao estresse. Uso não médico de remédio prescrito, ansiedade generalizada, sintomas depressivos e pior qualidade de sono e de vida foram identificados como possíveis consequências do estresse. Conclusões: O modelo ENEM/SiSU parece não exercer efeito no estresse. Além disso, esse desfecho foi mais elevado entre indivíduos mais vulneráveis, tendo contribuído para diversos desfechos negativos em saúde mental. Recomenda-se o fortalecimento de serviços de assistência estudantil para que esses casos sejam identificados e manejados. Objetivo graduandos Métodos 201 99 Brasil estágio Percebido Poisson respectivamente Resultados 311 31 1 31, DP 9. 9 . 9) feminino heterossexual pobres alimentar bairro estressados atual ingresso prescrito generalizada Conclusões ENEMSiSU ENEM SiSU disso vulneráveis mental Recomendase Recomenda se manejados 20 3 2

RESUMO O objetivo desta pesquisa foi comparar o efeito dos seguintes produtos sobre bovinos leiteiros, parasitados por Rhipicephalus microplus: organossintéticos - clorfenvinfós e ivermectina (OG); fitoterápicos - óleo de eucalipto (EG) e torta de Neem (NG) e homeopatia (HG). O grupo controle (CG) não recebeu tratamento. Foram avaliados infestação por R. microplus (MIC) e ganho de peso/grupo (GP) em 60 animais (5 grupos), a partir de – holandês/zebu, com idade entre 25 e 44 meses e peso inicial entre 211 e 477kg. EG e NG apresentaram MIC 84,9% e 14,0% maior que CG, respectivamente (P<0,05; P=0). HG e OG apresentaram MIC menor que CG 24,4% e 16,9%, respectivamente (P<0,05; P<0,05). EG, NG e OG obtiveram GP menor que CG em 7,9%, 8,9% e 8,06% (P<0,05), respectivamente. O GP do HG foi 4,9% maior (P<0,05) que o CG. Esta foi a primeira pesquisa a comprovar controle do parasitismo por R. microplus em teste a campo, usando homeopatia. A metodologia para escolha de medicamentos homeopáticos para controle de enzootias, desenvolvida e testada pela primeira vez neste estudo, mostrou-se adequada e eficiente, abrindo a possibilidade para estabelecimento de nova metodologia para controle estratégico do parasitismo por R. microplus.

ABSTRACT The goal of this research was to compare the effect of the following products on dairy cattle, parasitized by Rhipicephalus microplus: organosynthetics - Clorfenvinfós and Ivermectina (OG); phytotherapics - Eucalyptus oil (EG) and Neem cake (NG); Homeopathy (HG); The control group (CG) received no treatment. Infestation was by R. microplus (MIC) and weight gain (GP) were evaluated. The study included 60 animals (5 groups) from – Dutch / Zebu, aged between 25-44 months and initial weight between 211-477kg. EG and NG showed MIC 84.9% and 14.0% greater than CG, respectively (P <0.05; P = 0). HG and OG had MIC less than CG 24.4% and 16.9%, respectively (P<0.05; P <0.05). EG, NG and OG obtained GP lower than CG in 7.9%, 8.9% and 8.06% (P <0.05) respectively. The HG GP was 4.9% higher (P <0.05) than CG. This was the first research to prove parasitism control by R. microplus in a field test, using homeopathy. The methodology for choosing Homeopathy for the control of enzooties, developed and tested for the first time in this study, proved to be adequate and efficient, opening the possibility for establishing a new methodology for strategic control of parasitism by R. microplus.

ABSTRACT Objective Paraoxonase 1 (PON1) polymorphisms are associated with an increased susceptibility to cardiovascular disease. PON1 Q192R polymorphism (rs662) partially determine PON1 hydrolytic activity and protect against oxidation of LDL and HDL. This study aimed to delineate the association of PON1 status (functional 192 genotype and plasma activity levels) and atherogenicity in urbans residents aged 40 years or more. Materials and methods Anthropometric data, lipid profiles, the atherogenic index of the plasma (AIP) and Framingham score risk were measured. Three kinetic assays were conducted to assay PON1 status using phenylacetate and 4-(chloromethyl)phenyl acetate as substrates. Results Smoking per se did not significantly impact the AIP but the interaction PON1 genotype by smoking significantly increased the AIP. In subjects with the RR genotype smoking increased the AIP index from (estimated mean ± SEM) -0.038 ± 0.039 to 0.224 ± 0.094. The QR genotype increased the Framingham risk index by around 1.3 points. Smoking by RR genotype carriers significantly increased the Framingham risk score (17.23 ± 2.04) as compared to smoking (13.00 ± 1.06) and non-smoking (7.79 ± 0.70) by QQ+QR genotype carriers. The interaction RR genotype by smoking was a more important predictor (odds ratio = 7.90) of an increased Framingham risk score (> 20) than smoking per se (odds ratio = 2.73). The interaction smoking by RR genotype carriers significantly increased triglycerides and lowered HDL cholesterol. Conclusion Smoking per se has no (AIP) or a mild (Framingham risk score) effect on atherogenicity, while the interaction smoking by PON1 RR genotype has a clinically highly significant impact on atherogenicity.

Constraints to the sustainability of insecticide use include effects on human health, agroecosystems (e.g., beneficial insects), the wider environment (e.g., non-target species, landscapes and communities) and the selection of insecticideresistant traits. It is possible to find examples where insecticides have impacted disastrously on all these variables and others where the hazards posed have been (through accident or design) ameliorated. In this review, we examine what can currently be surmised about the direct and indirect long-term, field impacts of insecticides upon the environment. We detail specific examples, describe current insecticide use patterns, consider the contexts within which insecticide use occurs and discuss the role of regulation and legislation in reducing risk. We consider how insecticide use is changing in response to increasing environmental awareness and inevitably, as we discuss the main constraints to insecticide use, we suggest why they cannot easily be discarded.

Las restricciones de la sostenibilidad acerca del uso de insecticidas incluyen los efectos en la salud humana, los ecosistemas agrícolas (ejemplo, los insectos beneficiosos), el medio ambiente, en su sentido más amplio (por ejemplo, las especies que no son el objetivo, paisajes y comunidades) y la selección de los rasgos que confieren la resistencia a los insecticidas. Es posible encontrar ejemplos donde los insecticidas han tenido un impacto desastroso en todas aquellas variables y otros ejemplos donde los peligros que representaban han sido mitigados (por accidente o por diseño). En esta revisión examinamos lo que en la actualidad se puede concluir sobre el impacto de campo directo e indirecto y de largo plazo de los insecticidas en el medio ambiente. Proporcionamos ejemplos específicos, describimos los patrones actuales del uso de insecticidas, consideramos los contextos donde se usan los insecticidas y discutimos el papel de los reglamentos y leyes a fin de mitigar el riesgo. Discutimos cómo el uso de los insecticidas está cambiando como resultado de una mayor conciencia ambiental e inevitablemente, mientras discutimos las principales restricciones del uso de los insecticidas, también sugerimos por qué no se pueden descartar tan fácilmente.

Testou-se a atividade larvicida de 33 derivados arilsulfonílicos da (+)-cânfora e da (+)-isopinocanfona no carrapato B. microplus, na busca de princípios ativos menos tóxicos para o seu controle. Os produtos foram obtidos por clorossulfonação da (+)-cânfora e da (+)-isopinocanfona. Eles foram submetidos à solubilização e testados separadamente e em conjunto contra larvas de carrapato encerradas em envelopes contendo papéis impregnados e acondicionadas em estufa climatizada. A mortalidade média não atingiu 5% em todos os testes realizados, indicando que a clorossulfonação não é a rota de síntese mais adequada para a obtenção de derivados sintéticos com efeito larvicida sobre B. microplus. Os 33 produtos testados sob a forma de triagem biológica não podem ser considerados como potenciais acaricidas.

It was investigated the acaricidal activity of (+)- camphor and (+)- isopinocamphone arilsulphonyl derivatives against Boophilus microplus cattle tick. The products were obtained through the camphor and isopinocamphone clorosulfonation. Thirty-three products were submitted to solubilization and tested alone and together, against tick larvae. Ticks were caught in filter paper envelopes impregnated with products, which were incubated under controlled conditions. The average mortality did not reach 5% in all trials, indicating that clorosulfonation is not an appropiate procedure to obtain derivatives with larvicidal effect against B. microplus. The 33 products tested in the biological screening could not be considered as potential acaricides.