Abstract Introduction: Screen use among adolescents has increased, with excessive screen time associated with poor sleep. Isolation due to the COVID-19 pandemic may have exacerbated these problems. This study aimed to characterize adolescents’ sleep and screen use behaviors during the COVID-19 pandemic lockdown. Methods: This was a multicenter, cross-sectional, descriptive study of adolescents aged 10 to 18 years evaluated in an Adolescent Medicine outpatient visit of four hospitals in northern Portugal between January and March 2021. Adolescents completed a survey with questions about screen use and sleep and the Pediatric Daytime Sleepiness Scale (Portuguese version). Results: A total of 131 adolescents (66.4% female; median age 15 years) were enrolled during the study period. Participants reported a median sleep duration of nine hours on weekdays and 10 hours on weekends, with 25.9% sleeping less than eight hours on weekdays. Approximately 80% reported good or very good sleep quality. Onset insomnia was identified in 39.7% and excessive daytime sleepiness (EDS) in 13.7%. Most adolescents (74.0%) reported screen time of ≥6 hours/day. Screens were mainly used for attending classes and talking to friends. Smartphones were available in the bedroom at night for 83.2%, while 61.1% had televisions, 57.3% had computers, and 16.0% had gaming consoles in their bedrooms. Fifty-five percent used screens within an hour of bedtime every day. Screen time ≥6 hours/day and having a computer or smartphone in the bedroom at night were associated with shorter sleep duration on weekdays and EDS. Screen use within an hour of bedtime ≥4 times/week was associated with sleep-onset insomnia, shorter weekday sleep duration, and EDS. Discussion: Online classes during the COVID-19 lockdown may explain the use of devices ≥6 hours/day. Although most adolescents reported normal median sleep time and subjective good or very good sleep quality, screen use ≥6 hours/day and within one hour before bedtime was associated with sleep deprivation on weekdays and EDS. Conclusions: Excessive screen time during the pandemic appears to have had a negative impact on adolescents’ sleep. These findings underscore the importance of identifying and intervening in these issues when addressing adolescent global health.

Resumo Introdução: O uso de ecrãs tem vindo a aumentar entre os adolescentes, estando descrita a associação entre tempo excessivo de ecrã e menor quantidade e pior qualidade de sono. A pandemia de COVID-19 poderá ter agravado estes problemas. Este estudo pretendeu caracterizar o comportamento dos adolescentes relativamente ao sono e uso de ecrãs durante o confinamento imposto pela pandemia de COVID-19. Métodos: Estudo descritivo, transversal, multicêntrico de adolescentes dos 10 aos 18 anos avaliados numa consulta externa de Medicina do Adolescente de quatro hospitais do norte de Portugal entre janeiro e março de 2021. Os adolescentes preencheram um questionário com perguntas sobre o uso de ecrãs e o sono e a Pediatric Daytime Sleepiness Scale (versão portuguesa). Resultados: Foram incluídos 131 adolescentes (66,4% do género feminino; idade mediana de 15 anos) durante o período de estudo. Os participantes relataram uma mediana de duração do sono de 9 horas durante a semana e 10 horas ao fim-de-semana, com 25,9% a dormir menos do que oito horas diárias durante a semana. Cerca de 80% referiu ter um sono de boa ou muito boa qualidade. Foi identificada insónia inicial em 39,7% e sonolência diurna excessiva (SDE) em 13,7% dos participantes. A maioria (74,0%) referiu um tempo de ecrã ≥6 horas/dia. Os ecrãs foram usados maioritariamente para assistir a aulas e conversar com amigos. Um total de 83,2% dos adolescentes referiu dormir com o telemóvel no quarto e 61,1% referiu ter televisão, 57,3% computador e 16,0% consola de jogos no quarto. Cinquenta e cinco por cento usava ecrãs todos os dias na hora antes de dormir. Um tempo de ecrã ≥6 horas/dia e ter computador ou telemóvel no quarto durante a noite associou-se a uma menor duração do sono durante a semana e SDE. O uso de ecrãs na hora antes de dormir associou-se a insónia inicial, menor duração do sono durante a semana e SDE. Discussão: As aulas online poderão explicar o uso frequente de ecrãs (≥6 horas/dia). Embora a mediana de duração do sono tenha sido normal e a maioria dos adolescentes tenha referido ter um sono de boa ou muito boa qualidade, o uso de ecrãs ≥6 horas/dia e na hora antes de dormir associou-se a privação de sono durante a semana e SDE. Conclusão: O uso excessivo de ecrãs durante a pandemia parece ter tido um impacto negativo no sono dos adolescentes. Estes dados reforçam a necessidade de identificar e intervir nestes problemas durante a abordagem de saúde global do adolescente.

Resumo A síndrome VEXAS (vacúolos, enzima E1, cromossoma X, autoinflamação, mutação somática) foi identificada em 2020 por Beck et al, ao analisar o genoma de 2560 doentes com diferentes tipos de manifestações autoinflamatórias. Fizemos uma revisão da literatura com o objetivo de divulgar a evidência científica existente e aumentar o grau de suspeição clínica da comunidade médica. Atualmente, estão identificadas várias mutações associadas a esta síndrome, originando defeitos na ubiquitinação e ativação do sistema imune inato. Da análise fenotípica dos portadores da mutação, foram encontradas características comuns: sexo masculino, idade superior a 50 anos, citopenias, febre e sintomas constitucionais, podendo estar envolvidos todos os órgãos e sistemas com manifestações e proporções variáveis. A natureza inflamatória e multissistémica desta doença representa uma dificuldade acrescida no seu diagnóstico e tratamento, cursando com elevada morbimortalidade. Atualmente, os tratamentos com maior eficácia demonstrada são corticoterapia, azacitidina, inibidores da JAK-1/2 e inibidores da interleucina-6, parecendo também existir benefício com o transplante autólogo de células estaminais. Tendo em conta a sua identificação recente e baixo número de doentes diagnosticados, a maioria dos estudos disponíveis apresentam limitações relacionadas com a metodologia e curta duração de seguimento, imperando a necessidade de ensaios clínicos prospetivos de modo a definir as melhores estratégias terapêuticas e melhorar o prognóstico destes doentes.

Abstract VEXAS (vacuoles, E1 enzyme, X-linked, autoinflammatory, somatic) syndrome was identified in 2020 by Beck et al, when analyzing the genome of 2560 patients with different types of autoinflammatory manifestations. A literature review was carried out with the aim of disseminating existing scientific evidence and increasing the degree of clinical suspicion of the medical community. Currently, many mutations associated with this syndro-me have been identified, causing defects in the ubiquitylation and activation of the innate immune system. From the phenotypic analyses of the mutation carriers, some common features were found: male, over 50 years old, cytopenias, fever and constitutional symptoms, and all organs and systems with variable manifestations and proportions may be involved. The inflammatory and multi-systemic nature of this disease leads to a diagnostic and treatment challenge, adding to the already existing high morbimortality. Presently, the treatments with greater efficiency are corticosteroid therapy, azacytidine, JAK-1/2 inhibitors and interleukin-6 inhibitors. There are also benefits in autologous stem cell transplants. Given its recent identification and low number of diagnosed patients, most available studies have limitations related to the methodology and short duration of follow-up, prevailing the need for prospective clinical trials in order to define the best therapeutic strategies and to improve these patients’ prognosis.

RESUMEN La investigación realizada tuvo como objetivo proponer un Sistema de Medios de enseñanza y aprendizaje en Formato Digital, para el estudio de la Zoología de los invertebrados, que se imparte en la Licenciatura en Educación. Biología en el 2do año del Curso Regular Diurno, de la Facultad de Educación Media del Campus «Félix Varela Morales» en la Universidad Central «Marta Abreu» de Las Villas, Villa Clara, Cuba. Para el desarrollo de esta se utilizaron métodos de recopilación de la información y de procesamiento de la información recopilada como, observación participativa, revisión de documentos, encuesta, entrevista, criterios de expertos, histórico-lógico, analítico-sintético, deductivo-demostrativo, sistémico-estructural, ascenso de lo abstracto a lo concreto, y la modelación. Se utilizan, además, métodos estadísticos y matemáticos. La muestra seleccionada estuvo integrada por 32 estudiantes que se corresponde con la matricula del 2do año del Curso Regular Diurno de la carrera de Licenciatura en Educación. Biología y 10 profesores de diferentes universidades de Cuba. El sistema fue valorado por dos grupos de expertos como pertinente, y se introdujo en el curso 2017-2018, con excelentes resultados. Se concluye que con el sistema se logra una participación más activa en la formación de los conocimientos y una libertad espacio-temporal.

ABSTRACT The research carried out had as objective to propose a Media System for teaching and learning in Digital Format, for the study of Zoology of invertebrates, which is taught in the Bachelor's Degree in Education. Biology in the 2nd year of the Regular Day Course, of the Faculty of Secondary Education of the Campus "Félix Varela Morales" at the Central University "Marta Abreu" of Las Villas, Villa Clara, Cuba. For the development of this study, methods of information collection and processing of the information collected were used, such as participatory observation, document review, survey, interview, expert criteria, historical-logical, analytical-synthetic, deductive-demonstrative, systemic-structural, ascent from the abstract to the concrete, and modeling. Statistical and mathematical methods are also used. The selected sample consisted of 32 students corresponding to the enrollment of the 2nd year of the Regular Day Course of the Bachelor's Degree in Education. Biology and 10 professors from different universities in Cuba. The system was evaluated by two groups of experts as pertinent, and it was introduced in the 2017-2018 course, with excellent results. It is concluded that with the system a more active participation in the formation of knowledge and a spatio-temporal freedom is achieved.

Abstract Background Vitiligo is the most common pigmentary disorder and is considered a chronic, cumulative, multifactorial disease. The crucial role of cytotoxic CD8+ T lymphocytes and the IFNγ/CXCL10 axis has been demonstrated in its pathogenesis. Objective To evaluate the clinical profile and immuno-inflammatory markers in patients with vitiligo in a reference medical center. Methods Cross-sectional study in which all patients with vitiligo seen at the medical center the from 2019 to 2022 were evaluated, to outline the clinical profile. Moreover, cardiovascular risk biomarkers (neutrophil/lymphocyte ratio and C-reactive protein levels) were measured, as well as cytokines and chemokines (TNFα, IFNγ, IL10, IL15 and CXCL10) in the serum of a subgroup of 30 patients. Results There was a predominance of females, with a mean age of 43 years. Most were phototypes IV or V (71.3%), without comorbidities (77.55%), and without a family history of vitiligo (70.41%). Higher levels of neutrophil/lymphocyte ratio, C-reactive protein, and inflammatory cytokines/chemokines were documented in vitiligo patients when compared to the control group (non-significant). As relevant data, the highest values of CXCL10 were detected in patients with vitiligo versus controls, as well as in patients with disease of shorter duration (p < 0.05). Study limitations The number of assessed patients was small due to recruitment difficulties caused by the COVID-19 pandemic. Conclusion The present data contribute to confirming the relevant role of the IFNγ/CXCL10 axis in the pathogenesis of vitiligo, highlighting CXCL10 as a possible activity marker. chronic cumulative CD8 CD IFNγCXCL10 IFNγCXCL IFNγ CXCL IFNγ/CXCL1 immunoinflammatory immuno Crosssectional Cross sectional 201 202 evaluated Moreover neutrophillymphocyte neutrophil lymphocyte Creactive C reactive measured TNFα, TNFα (TNFα IL10 IL IL1 3 females 4 years 71.3%, 713 71.3% , 71 (71.3%) 77.55%, 7755 77.55% 77 55 (77.55%) 70.41%. 7041 70.41% . 70 41 (70.41%) cytokineschemokines nonsignificant. nonsignificant non significant (non-significant) CXCL1 controls p 0.05. 005 0.05 0 05 0.05) COVID19 COVID 19 COVID-1 pandemic marker IFNγCXCL1 IFNγ/CXCL 20 71.3 7 (71.3% 775 77.55 5 (77.55% 704 70.41 (70.41% (non-significant 00 0.0 COVID1 1 COVID- 2 71. (71.3 77.5 (77.55 70.4 (70.41 0. (71. 77. (77.5 70. (70.4 (71 (77. (70. (7 (77 (70 (

Composites prepared from metal oxides and materials derived from agroforestry residues have been successfully applied to remove emerging contaminants. The lignin properties, high surface area and number of functional groups, are beneficial in the preparation of TiO2 composites. In the present work, sisal residue was used as a source of lignin, and after extraction, the lignin was applied in the preparation of an adsorbent composite with TiO2 to remove bisphenol-A. The optimal conditions for lignin extraction resulted in a lignin purity of 73%. Characterization proved that the synthesized composites have a high surface area (> 100 m2 g-1), homogeneous distribution of TiO2 particles and stable surface charge. Such characteristics resulted in a lignin/TiO2 composite with a maximum adsorption capacity of 13.7 mg L-1, capable of removing up to 97% of bisphenol-A. The adsorbent can be used in six cycles, removing over 70% by the third cycle, which indicates good stability. contaminants properties groups TiO work bisphenolA. bisphenolA bisphenol A. A bisphenol-A 73 73% > ( 10 m g1, g1 g 1 , g-1) charge ligninTiO2 ligninTiO lignin/TiO 137 13 7 13. L1, L1 L 1, L-1 97 cycles 70 cycle stability g-1 L- 9 g-

ABSTRACT Background: The current treatments for Chagas disease (CD) include benznidazole and nifurtimox, which have limited efficacy and cause numerous side effects. Triazoles are candidates for new CD treatments due to their ability to eliminate T. cruzi parasites by inhibiting ergosterol synthesis, thereby damaging the cell membranes of the parasite. Methods: Eleven synthetic analogs of the kinase inhibitor SRPIN340 containing a triazole core (compounds 6A-6K) were screened in vitro against the Tulahuen strain transfected with β-galactosidase, and their IC50, CC50, and selectivity indexes (SI) were calculated. Compounds with an SI > 50 were further evaluated in mice infected with the T. cruzi Y strain by rapid testing. Results: Eight compounds were active in vitro with IC50 values ranging from 0.5-10.5 µg/mL. The most active compounds, 6E and 6H, had SI values of 125.2 and 69.6, respectively. These compounds also showed in vivo activity, leading to a reduction in parasitemia at doses of 10, 50, and 250 mg/kg/day. At doses of 50 and 250 mg/kg/day, parasitemia was significantly reduced compared to infected untreated animals, with no significant differences between the effects of 6E and 6H. Conclusions: This study identified two new promising compounds for CD chemotherapy and confirmed their activity against T. cruzi. Background (CD nifurtimox T synthesis parasite Methods SRPIN SRPIN34 6A6K AK 6A 6K A K 6A-6K βgalactosidase, βgalactosidase β galactosidase, galactosidase β-galactosidase IC CC50 CC (SI calculated 5 testing Results IC5 0.510.5 05105 0.5 10.5 0 10 0.5-10. µgmL µg mL µg/mL E 6H H 1252 125 2 125. 696 69 6 69.6 respectively 25 mgkgday mg kg day mg/kg/day animals Conclusions SRPIN3 CC5 510 0.510. 0510 05 0. 105 10. 1 0.5-10 12 69. 51 0.510 051 0.5-1 0.51 0.5-

Abstract Hemophagocytic lymphohistiocytosis (HLH) is a rare genetic hyperinflammatory syndrome that occurs early in life. Macrophage activation syndrome (MAS) usually refers to a secondary form of HLH associated with autoimmunity, although there are other causes of secondary HLH, such as infections and malignancy. In this article, we reviewed the concepts, epidemiology, clinical and laboratory features, diagnosis, differential diagnosis, prognosis, and treatment of HLH and MAS. We also reviewed the presence of MAS in the most common autoimmune diseases that affect children. Both are severe diseases that require prompt diagnosis and treatment to avoid morbidity and mortality. (HLH life (MAS autoimmunity malignancy article concepts epidemiology features prognosis children mortality

Resumo Esta revisão sistemática tem como objetivo avaliar a relação entre atividade física (AF)/exercício físico (EF)/desporto, saúde mental (SM) e percepção de qualidade de vida (QV) de estudantes matriculados no ensino superior. As bases de dados utilizadas para a seleção dos artigos foram Pubmed, Web of Science, Scopus e SPORTDiscus e os descritores foram “university student”, “university student”, “higher education student”, “physical activity”, “sports”, “exercise”, “treino”, “humor depressivo”, “stress”, “ansiedade” e “qualidade de vida”. Seguindo os procedimentos metodológicos do fluxograma PRISMA, foram selecionados 37 artigos e, destes, 16 atenderam aos critérios de inclusão do estudo. Nesta revisão sistemática foram registados efeitos benéficos da prática de AF/EF/desporto na SM e na percepção da QV. Nos estudos incluídos nesta revisão sistemática a ansiedade, a depressão e o stress foram menores nos indivíduos que praticavam AF/EF/desporto e, quanto maior a sua prática, menores os sintomas dessas comorbidades. Ao mesmo tempo, houve também uma correlação significativa entre AF/EF/desporto e SM. Por outro lado, níveis mais elevados de AF/EF/desporto também foram relacionados à melhor percepção de QV. Essas evidências revelam a relação entre AF/EF/desporto com a SM e a percepção de QV dos estudantes matriculados no ensino superior. É importante seguir as recomendações da Organização Mundial da Saúde (2020) para a prática de AF/EF/desporto para promover estilos de vida saudáveis nos estudantes matriculados no ensino superior e promover a sua perceção de SM e QV.

Abstract This systematic review aims to evaluate the relationship between physical activity (PA)/physical exercise (PE)/sport, mental health (MH) and quality of life perception (QoL) of students enrolled in higher education. The databases used for the articles selection were Pubmed, Web of Science, Scopus and SPORTDiscus and descriptors were “university student”, “university student”, “higher education student”, “physical activity”, “sports”, “exercise”, “training”, “depressive mood”, “stress”, “anxiety”, and “quality of life”. Following the process provided by the PRISMA flow diagram, 37 articles were selected and, of these, 16 met the inclusion criteria for the study. In this systematic review the beneficial effects of the practice of PA/PE/sport on MH and on the QoL perception was registered. In the studies include in this systematic review anxiety, depression, and stress were lower in individuals who practiced PA/PE/sport and the greater their practice, the lower the symptoms of such comorbidities. At the same time there was also a significant correlation between PA/PE/sport and MH. On the other hand, higher levels of PA/PE/sport were also related to better QoL perception. This evidence reveals the relationship between PA/PE/sport with MH and the QoL perception of students enrolled in higher education. It is important to follow the World Health Organization (2020) recommendations for PA/PE/sport practice to further healthy lifestyles in students enrolled in higher education and to promote their MH and QoL perception.

Annual world production of sisal is estimated to be 400,000 tons, but the processing of sisal fiber generates around 160,000 tons of waste per year. The sisal bagasse is discarded, even though it is about 60% carbohydrates in its structure. Therefore, this work seeks to add value to sisal bagasse through the production of cellulose acetate membranes aimed at removing inhibitors from the fermentation process. The membrane was developed using a cellulose acetate polymer synthesized from cellulose isolated from sisal bagasse. Alkaline and acid pre-treatments resulted in 90% pure cellulose. The obtained cellulose acetate was confirmed by infrared spectroscopy. In the synthesis of the membranes, the influence of agitation time and temperature of the bath on the selectivity and porosity were evaluated in four tests, the third condition stood out with porosity of 26.23% and selectivity of 94.51% in the removal of the furfural and 36.62% of the acetic acid. 400000 400 000 400,00 160000 160 160,00 year discarded 60 structure Therefore process pretreatments pre treatments 90 spectroscopy tests 2623 26 23 26.23 9451 94 51 94.51 3662 36 62 36.62 40000 40 00 400,0 16000 16 160,0 6 9 262 2 26.2 945 5 94.5 366 3 36.6 4000 4 0 400, 1600 1 160, 26. 94. 36.

Abstract Introduction: This study aimed to assess the clinical, economic, and humanistic impact of short-bowel syndrome/chronic intestinal failure (SBS/CIF) in Portugal. Methods: This is a retrospective multicenter cohort chart review study, with a cross-sectional component for quality-of-life (QoL) evaluation. Inclusion criteria comprised patients with SBS/CIF, aged ≥1 year, with stable parenteral nutrition (PN). Data collection included patient chart review over a 12-month period and patient/caregiver self-report and SF-36/PedsQL™ questionnaires. Main endpoints comprised clinical and PN characterization, healthcare resource use (HRU), direct costs, and patient QoL. Results: Thirty-one patients were included (11 adults and 20 children). Patients’ mean age (standard deviation [SD]) was 57.9 (14.3) years in adults and 7.5 (5.0) years in children, with a mean time since diagnosis of 10.2 (5.9) and 6.6 (4.2) years, respectively. PN was administered for a mean of 5.2 and 6.6 days/week in adults and children, respectively; home PN occurred in 81.8% of adults and 90.0% of children for a mean of 9.6 and 10.8 months/year, respectively. The mean annual number of hospitalizations was 1.9 and 2.0 which lasted for a mean of 34.0 and 29.4 days in adults and children, respectively. Twenty-one and forty hospitalization episodes were reported in adults and children, respectively, of which 71.4% and 85.0% were due to catheter-related complications. Mean annual direct costs per patient amounted to 47,857.53 EUR in adults and 74,734.50 EUR in children, with PN and hospitalizations as the main cost-drivers. QoL assessment showed a clinically significant impaired physical component in adults and a notable deterioration in the school functioning domain in children. Conclusion: In Portugal, SBS/CIF patient management is characterized by a substantial therapeutic burden and HRU, translating into high direct costs and a substantial impairment of the adults’ physical function and children’s school functioning.

Resumo Introdução: Este estudo teve como objetivo avaliar o impacto clínico, económico e social da síndrome do intestino curto/falência intestinal crónica (SIC/FIC) em Portugal. Métodos: Estudo de coorte retrospectivo e multicêntrico de revisão dos processos clínicos incluindo uma componente transversal para avaliação da qualidade de vida (QV). Os critérios de elegibilidade incluíram doentes com SIC/FIC, idade ≥1 ano, em nutrição parenteral (NP) e clinicamente estáveis. A recolha de dados incluiu a revisão dos processos clínicos ao longo de um período de 12 meses e a aplicação de questionários auto-administrados a doentes e cuidadores e de questionários de QV (SF-36/ PedsQL™). Os indicadores principais foram a caracterização clínica e da NP, a utilização de recursos de saúde, custos diretos e QV dos doentes. Resultados: Foram incluídos 31 doentes (11 adultos e 20 crianças). A idade média (desvio padrão: DP) foi de 57.9 (14.3) anos nos adultos e de 7.5 (5.0) nas crianças com um tempo médio desde o diagnóstico de 10.2 (5.9) e 6.6 (4.2) anos, respetivamente. A NP foi administrada durante uma média de 5.2 e 6.6 dias por semana, em adultos e crianças respetivamente, em 81.8% e 90.0% dos adultos/crianças foi feita em casa durante uma média de 9.6 ou 10.8 meses por ano, respetivamente. O número médio anual de hospitalizações foi de 1.9 (1.6) e 2.0 (1.5) com uma duração média de 34.0 (47.4) e 29.4 (32.3) dias, em adultos e crianças, respetivamente. Foram reportados 21 e 40 episódios de hospitalização em adultos/crianças, dos quais 71.4% e 85.0% foram devido a complicações relacionadas ao uso de cateter. Os custos diretos anuais médios por doente ascenderam a 47,857.53 EUR nos adultos e a 74,734.50 EUR nas crianças, sendo que os maiores responsáveis foram a NP e as hospitalizações. A avaliação da QV mostrou um comprometimento clinicamente significativo da componente física nos adultos e uma deterioração relevante da dimensão escolar nas crianças. Conclusões: A gestão dos doentes com SIC/FIC em Portugal é caracterizada por uma sobrecarga substancial a nível terapêutico e de utilização de recursos de saúde, o que se traduz em elevados custos diretos e comprometimento substancial da componente física nos adultos e do desempenho escolar nas crianças.

This work presents a study on the interference of kaolinite particles, smaller than 10 µm, in the performance of the cationic flotation of quartz. The surface properties control the effects of heteroaggregation and provide the creation of a coverage of these ultrafine on the coarser particle surfaces (slime coating), which makes it difficult for the collectors to adsorb and/or contact the air bubbles. The flotation experiments revealed that the etheramine collector used is capable of recovering 76.9% of ultrafine kaolinite in the absence of coarse quartz particles. On the other hand, the flotation results carried out with the binary mixture (quartz −150 + 45 µm and kaolinite ultrafines −6 µm) and in the presence of the same reagent system showed that kaolinite floats together with quartz depending on the content of ultrafines in the suspension. Micrographs from MEV/EDS showed that a heterocoagulation between coarse quartz particles and kaolinite ultrafine with a formation of hydrophobic agglomerates can be recovered in the flotation. 1 slime coating, coating , coating) andor or bubbles 769 76 9 76.9 hand 150 −15 4 6 − suspension MEVEDS MEV EDS 7 76. 15 −1

Resumen Este estudio tuvo como objetivo describir el primer curso presencial para capacitar a los profesionales de la salud pública para llevar a cabo la vigilancia genómica en tiempo real durante los períodos de pandemia. Este es un informe de experiencia en un curso teórico-práctico centrado en la investigación y vigilancia genómica, que incluye secuenciación móvil, bioinformática, filogenética y tecnologías de modelado epidemiológico. Este evento contó con la asistencia de 162 participantes y fue la primera gran capacitación presencial realizada durante la epidemia de COVID-19 en Brasil. No se detectó infección por SARS-CoV-2 al final del evento en ningún participante, lo que sugiere la seguridad y efectividad de todas las medidas de seguridad adoptadas. Por lo tanto, los resultados del evento sugieren que es posible realizar entrenamientos profesionales de manera segura durante pandemias, siempre y cuando se sigan todos los protocolos de seguridad. pandemia teóricopráctico teórico práctico móvil bioinformática epidemiológico 16 COVID19 COVID 19 COVID-1 Brasil SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- participante adoptadas tanto pandemias 1 COVID1 COVID- SARS-CoV

Abstract The objective of this report was to describe the first face-to-face course aimed at training public health professionals in performing real-time genomic surveillance during the pandemic period. Experience report on a theoretical-practical course focusing on genomic research and surveillance, including mobile sequencing technologies, bioinformatics, phylogenetics and epidemiological modeling. There were 162 participants in the event and it was the first major face-to-face training course conducted during the COVID-19 epidemic in Brazil. No cases of SARS-CoV-2 infection was detected among the participants at the end of the event, suggesting the safety and effectiveness of all safety measures adopted. The results of this experience suggest that it is possible to conduct professional training safely during pandemics, as long as all safety protocols are followed. facetoface face realtime real time period theoreticalpractical theoretical practical technologies bioinformatics modeling 16 COVID19 COVID 19 COVID-1 Brazil SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- adopted pandemics followed 1 COVID1 COVID- SARS-CoV

Resumo O relato descrebeu o primeiro curso presencial visando capacitar profissionais de saúde pública na realização de vigilância genômica em tempo real, durante períodos pandêmicos. Relato de experiência sobre um curso teórico-prático com foco em pesquisa e vigilância genômica, incluindo tecnologias de sequenciamento móvel, bioinformática, filogenética e modelagem epidemiológica. O evento contou com 162 participantes e foi o primeiro grande treinamento presencial realizado durante a epidemia de covid-19 no Brasil. Não foi detectada infecção pelo SARS-CoV-2 ao final do evento em nenhum participante, sugerindo a segurança e efetividade de todas as medidas de segurança adotadas. Os resultados do evento sugerem que é possível executar capacitação profissional com segurança durante pandemias, desde que seguidos todos os protocolos de segurança. real pandêmicos teóricoprático teórico prático móvel bioinformática epidemiológica 16 covid19 covid 19 covid-1 Brasil SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- participante adotadas pandemias 1 covid1 covid- SARS-CoV

Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection of milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered milk samples received increasing concentrations of cheese whey (0, 1, 2.5, 5, 10, 20, 30, 50, and 100% v/v), and were subjected to lab-on-a-chip electrophoresis and SDS-PAGE to detect their mixtures. The lab-on-a-chip methodology was able to separate and quantify milk proteins. In addition, the tested technique is easy, rapid, sensitive, and can detect the addition of cheese whey in milk from the lowest level tested (1%) for milk proteins α-casein and β-casein. labonachip lab on a SDSPAGE. SDSPAGE SDS PAGE. PAGE Raw pasteurized ultrahigh ultra high UHT, UHT , (UHT) 0, 0 (0 1 25 2 5 2.5 10 20 30 50 100 v/v, vv v/v v v/v) mixtures easy rapid sensitive 1% (1% αcasein α casein βcasein. βcasein β casein. β-casein (UHT ( 2. 3 (1

Resumo O objetivo deste trabalho foi avaliar a eletroforese com dispositivo de microfluidos, conhecida como técnica lab-on-a-chip, para detecção de adulteração de leite com soro de queijo, em comparação ao SDS-PAGE. Amostras de leite cru, pasteurizado, processado em temperatura ultra-alta (UHT) e em pó receberam adição de soro de queijo em concentrações crescentes (0, 1, 2,5, 5, 10, 20, 30, 50 e 100% v/v) e foram submetidas a eletroforese lab-on-a-chip e SDS-PAGE para detectar suas misturas. A metodologia lab-on-a-chip foi capaz de separar e quantificar as proteínas do leite. Além disso, a técnica lab-on-a-chip é fácil, rápida, sensível e pode detectar adição de soro de queijo no leite do nível mais baixo testado (1%) para as proteínas do leite α-caseína e β-caseína. microfluidos labonachip, labonachip lab on chip, chip SDSPAGE. SDSPAGE SDS PAGE. PAGE cru pasteurizado ultraalta ultra alta UHT (UHT 0, 0 (0 1 25 2 5 2,5 10 20 30 100 v/v vv v misturas disso fácil rápida 1% (1% αcaseína α caseína βcaseína. βcaseína β caseína. β-caseína ( 2, 3 (1

RESUMEN Objetivo: este estudio tiene como objetivo identificar - en los profesionales de Cabo Verde que actúan en la Atención Primaria a la Salud - las barreras que en ese contexto dificultan la atención de personas con trastorno mental. Método: estudio cualitativo; los datos fueron recogidos entre febrero y marzo de 2020, por medio de entrevistas semiestructuradas, realizadas en 43 profesionales que trabajaban en cinco Centros de Salud, localizados en regiones urbanas de Cabo Verde, en África; posteriormente los datos fueron sometidos al análisis de contenido temático. Resultados: las barreras referidas fueron: la visión de los profesionales acerca de la persona con un trastorno mental que representa el sentimiento amenaza que coloca en riesgo su integridad física; la desarticulación entre el equipo y los servicios; el desconocimiento de los protocolos de atención; y, el distanciamiento en relación a los cuidados en el área de la salud mental. Conclusión: las barreras identificadas evidenciaron la necesidad de aumentar la calificación de los profesionales de la Atención Primaria; en ese sentido, una organización matricial puede ser un medio para la formación continuada, con el objetivo de potencializar los recursos existentes. Objetivo Método cualitativo 2020 semiestructuradas 4 África temático Resultados física servicios Conclusión sentido continuada existentes 202 20 2

ABSTRACT Objective: this study aimed to identify among Cape Verdean professionals working in Primary Health Care services the barriers impeding care from being provided to people with mental disorders in this context. Method: data in this qualitative study was collected between February and March 2020 using semi-structured interviews with 43 workers from five Health Centers in the urban areas of Cape Verde/Africa. The interviews were treated with content analysis. Results: the barriers included: the professionals’ perception regarding people with mental disorders concerning a feeling of being threatened by these patients, such as a fear of having their physical integrity at risk; lack of coordination between the team and the services; lack of knowledge of protocols; and distancing from care in the mental health field. Conclusion: the barriers identified here show a need to qualify PHC professionals. In this sense, matrix support is an alternative for continuing education to leveraging existing resources. Objective context Method 202 semistructured semi structured 4 VerdeAfrica Verde Africa Verde/Africa analysis Results included patients risk protocols field Conclusion sense resources 20 2

RESUMO Objetivo: este estudo tem como objetivo identificar junto aos profissionais caboverdianos, que atuam na Atenção Primária à Saúde as barreiras que, nesse contexto, dificultam o atendimento das pessoas com transtorno mental. Método: estudo qualitativo, cujos dados foram coletados entre fevereiro e março de 2020, por meio de entrevistas semiestruturadas, realizadas com 43 profissionais que trabalham em cinco Centros de Saúde localizados em regiões urbanas de Cabo Verde/África, e posteriormente submetidos à análise de conteúdo temática. Resultados: as barreiras referidas foram: a visão dos profissionais acerca da pessoa com transtorno mental representativa de sentimento de ameaça que coloca em risco a sua integridade física; a desarticulação entre a equipe e os serviços; o desconhecimento dos protocolos de atendimento e o distanciamento em relação aos cuidados na área de saúde mental. Conclusão: as barreiras identificadas evidenciam a necessidade de qualificação dos profissionais da Atenção Primária e, nesse sentido, o matriciamento pode ser uma via para a formação continuada, com vista a potencializar os recursos existentes. Objetivo caboverdianos contexto Método qualitativo 2020 semiestruturadas 4 VerdeÁfrica Verde África Verde/África temática Resultados física serviços Conclusão sentido continuada existentes 202 20 2