Abstract The promulgation of the bull Ineffabilis Deus in 1854 and with it the elevation of the mystery of the Immaculate Conception to dogma, constituted the prelude to the dogmatic proclamation of papal infallibility in 1870. The objective of this text is to analyze in a comparative way the political-religious scenarios that ruled both in Mexico and Spain and their transcendence for the Pase Regio or the Pase Nacional of this important bull that, theologically, put an end to the old spiritual discussions about the pure nature of Mary, but politically, it was the combative materialization of the papacy to recover its questioned temporal power.

Resumen La promulgación de la bula Ineffabilis Deus en 1854 y con ella la elevación del misterio de la Inmaculada Concepción a dogma, constituyó la antesala de la proclamación dogmática de la infalibilidad papal en 1870. El objetivo de este texto es analizar, de manera comparada, los escenarios político-religiosos que regían tanto en México como en España, así como la trascendencia para el Pase Regio o Pase Nacional de la importante bula que, en lo teológico, ponía fin a las añejas discusiones espirituales sobre la pura naturaleza de María, pero que en lo político constituyó la materialización de la lucha del papado por recuperar su cuestionado poder temporal.

Résumé La promulgation de la bulle papale Ineffabilis Deus en 1854, qui a élevé le mystère de l’Immaculée Conception au rang de dogme, a constitué le prélude à la proclamation dogmatique de l’infaillibilité du Pape en 1870. Ce texte vise à analyser de manière comparative l’ensemble des scénarios politico-religieux du Mexique et de l’Espagne, et la portée de cette importante bulle dans le cadre du Pase Regio ou du Pase Nacional. Théologiquement, celle-ci mettait fin à de longs débats spirituels sur la pureté de Marie, mais politiquement, elle matérialisait la lutte de la papauté pour réaffirmer son autorité temporelle, alors remise en question.

ABSTRACT BACKGROUND: Dynapenia is a risk factor of mortality. Therefore, the development of low-cost and easy-to-apply tools is essential to optimize the health surveillance actions of older people. OBJECTIVES: To compare the time spent on habitual physical activity (HPA) and sedentary behavior (SB) among dynapenic and non-dynapenic older adults and ascertain the predictive ability of these behaviors on outcome. DESIGN AND SETTING: A cross-sectional population epidemiological survey was conducted involving 208 older adults. METHODS: HPA and SB were quantified using the International Physical Activity Questionnaire, and dynapenia was identified by handgrip strength (women: 18.37 kgf; men: 26.75 kgf). RESULTS: The prevalence was 24.50%. In both sexes, dynapenic individuals reported a HPA median time of 70.00 minutes/week (min/wk), while non-dynapenic women and men reported HPA median times of 240.00 and 280.00 min/wk, respectively (P < 0.05). For SB among dynapenic individuals, a median of 388.75 min/day was observed in women and 428.57 min/d in men. In contrast, non-dynapenic women and men had 291.42 and 274.28 min/day in SB (P < 0.05), respectively. The best cutoff HPA to discriminate the outcome was 150.00 min/wk in women (sensitivity: 73.30%; specificity: 60.67%) and 140.00 min/wk in men (sensitivity, 71.43%; specificity, 61.54%). The best cutoff SB was 381.43 min/day in women (sensitivity, 53.30%; specificity, 84.80%) and 351.43 min/day in men (sensitivity, 71.43%; specificity, 73.85%). CONCLUSION: Older individuals with dynapenia spent less time on HPA and more time in SB. Furthermore, HPA was found to be a better discriminator of dynapenic individuals, and SB better discriminated non-dynapenic individuals. BACKGROUND mortality Therefore lowcost low cost easytoapply easy apply people OBJECTIVES (HPA (SB nondynapenic non SETTING crosssectional cross sectional 20 METHODS Questionnaire (women 1837 18 37 18.3 kgf 2675 26 75 26.7 kgf. . kgf) RESULTS 2450 24 50 24.50% sexes 7000 70 00 70.0 minutesweek minutes week minwk , min wk (min/wk) 24000 240 240.0 28000 280 280.0 P 0.05. 005 0.05 0 05 0.05) 38875 388 388.7 minday day 42857 428 57 428.5 mind d contrast 29142 291 42 291.4 27428 274 28 274.2 0.05, 15000 150 150.0 sensitivity (sensitivity 73.30% 7330 73 30 specificity 60.67% 6067 60 67 14000 140 140.0 sensitivity, 71.43% 7143 71 43 61.54%. 6154 61.54% 61 54 61.54%) 38143 381 381.4 53.30% 5330 53 84.80% 8480 84 80 35143 351 351.4 73.85%. 7385 73.85% 85 73.85%) CONCLUSION Furthermore 2 183 1 3 18. 267 7 26. 245 5 24.50 700 70. (min/wk 2400 240. 2800 280. 0.0 3887 38 388. 4285 428. 2914 29 4 291. 2742 27 274. 1500 15 150. 73.30 733 60.67 606 6 1400 14 140. 71.43 714 615 61.54 3814 381. 53.30 533 84.80 848 8 3514 35 351. 738 73.85 24.5 0. 73.3 60.6 71.4 61.5 53.3 84.8 73.8 24. 73. 60. 71. 61. 53. 84.

Résumé Ce travail présente les résultats d’une recherche dont l’objectif est de contribuer au développement de pratiques pédagogiques innovantes grace à l’installation d’un laboratoire d’expérimentation pédagogique (LAP) dans une école chilienne vulnérable. Suivant les principes de la recherche-action, 15 activités de formation concernant les pratiques innovantes ont été menées et une salle spécialisée a été mise en place. En parallèle, des entretiens, des groupes de discussion et des évaluations de processus ont été réalisés. Les résultats montrent que: (1) le potentiel de changement pédagogique; (2) de nouvelles façons de vivre et de penser l’espace de la salle de classe; (3) l’évaluation de l’expérimentation pédagogique. La conclusion est que l’innovation doit être un projet d’école et promouvoir une meilleure compréhension de l’apprentissage. dune d lobjectif l objectif linstallation installation dun dexpérimentation expérimentation LAP (LAP vulnérable rechercheaction, rechercheaction action, action recherche-action 1 place parallèle entretiens réalisés (1 2 (2 lespace espace classe 3 (3 lévaluation évaluation lexpérimentation linnovation innovation décole lapprentissage. lapprentissage apprentissage. apprentissage l’apprentissage (

Resumo Este texto apresenta os resultados de uma pesquisa cujo objetivo é contribuir para desenvolver práticas pedagógicas inovadoras por meio da instalação de um laboratório de experimentação pedagógica (LAP) em uma escola chilena vulnerável. De acordo com os princípios da pesquisa- -ação, foram realizadas 15 atividades formativas sobre práticas inovadoras e instalou-se uma sala especializada. Paralelamente realizaram-se entrevistas, grupos focais e avaliações de processo. Os resultados mostram o seguinte: (1) o potencial da mudança pedagógica; (2) novas formas de habitar o espaço das classes; (3) a avaliação da experimentação pedagógica. Conclui-se que a inovação deve ser um projeto escolar e deve promover uma ampla perspectiva para entender a aprendizagem. LAP (LAP vulnerável ação, ação -ação 1 instalouse instalou se especializada realizaramse realizaram entrevistas processo seguinte (1 2 (2 classes 3 (3 Concluise Conclui aprendizagem (

Abstract This paper presents the results of a research project which aimed to contribute to the development of innovative teaching practices by setting up a teaching experimentation laboratory (LAP) in a vulnerable Chilean school. According to the principles of action research, 15 training activities on innovative practices were carried out and a customized room was set up. Meanwhile, interviews, focus groups and process evaluations were carried out. The results show the following: (1) the potential of pedagogical change; (2) new ways of occupying the classroom space; (3) the evaluation of pedagogical experimentation. The conclusion is that innovation should be a school project and should promote a broad perspective for understanding learning. LAP (LAP 1 Meanwhile interviews following (1 change 2 (2 space 3 (3 learning (

Resumen Se presentan los resultados de una investigación cuyo objetivo es contribuir al desarrollo de prácticas pedagógicas innovadoras mediante la instalación de un laboratorio de experimentación pedagógica (LAP) en una escuela vulnerable en Chile. Siguiendo los principios de la investigación-acción, se realizaron 15 actividades formativas sobre prácticas innovadoras y se instaló una sala especializada. En paralelo, se hicieron entrevistas, grupos focales y evaluaciones de proceso. Los resultados muestran: (1) el potencial del cambio pedagógico; (2) nuevas formas de habitar el espacio de clases; (3) la valoración de la experimentación pedagógica. Se concluye que la innovación debe ser un proyecto de escuela y debe promover una perspectiva amplia para entender el aprendizaje. LAP (LAP Chile investigaciónacción, investigaciónacción acción, acción investigación-acción 1 especializada paralelo entrevistas proceso muestran (1 pedagógico 2 (2 clases 3 (3 aprendizaje (

Fluorescence spectroscopy is an extremely sensitive analytical technique that has been widely used to elucidate problems that require low detection limits. Although fluorescence spectroscopy is widespread in industry and academia, there is a lack of teaching materials demonstrating its basic principles, as well as the influence of instrumental parameters involved in the technique, which limit its correct use and the full potential of it. For this reason, this work aims to explore the principles of fluorescence spectroscopy and correlate them in function of different instrumental variables to carry out a fluorescence experiment. The spectrofluorometer parameters such as the excitation wavelength, excitation and emission slits, step, integration time, detection geometry as well as concentration of the samples were varied to illustrate how the best resulting spectra can be registered free of artifacts. To do so, two organic dyes with structured vibronic bands (pyrene) and another with unstructured bands (rhodamine 6G) were used. Another study carried out involved the use of 3D spectra, excitation/emission spectra, of cadmium telluride (CdTe) quantum dots. In this case, it was possible to show the excitation spectrum of nanomaterials, collecting emissions at different wavelengths, as well as analyzing the particle size distribution. limits academia reason experiment wavelength slits step time artifacts so pyrene (pyrene rhodamine 6G G D excitationemission CdTe (CdTe dots case nanomaterials wavelengths distribution

Abstract The prompt and accurate diagnosis of foot-and-mouth disease outbreaks is crucial to curb the swift transmission of its causative agent, the foot-and-mouth disease virus (FMDV), which poses a substantial threat to the nation’s livestock. This study aimed to perform a comparative analysis of various RT-qPCR methods for the detection of FMDV. The methods evaluated for the 3D region demonstrated similar sensitivity and specificity. Nevertheless, noticeable distinctions emerged in samples with low RNA concentration. While these methods can be interchangeably utilized with consistent outcomes when handling a large number of samples sent to the laboratory to pinpoint an outbreak, minor variations may assume significance when working with a limited quantity of samples containing low FMDV RNA copies. footandmouth foot mouth agent FMDV, , (FMDV) nations nation s livestock RTqPCR RT qPCR D specificity Nevertheless concentration outbreak copies (FMDV

Resumo O diagnóstico rápido e preciso de surtos de febre aftosa é crucial para conter a rápida transmissão de seu agente causador, o vírus da febre aftosa (FMDV), que representa uma ameaça significativa ao gado do país. Este estudo teve como objetivo realizar uma análise comparativa de vários métodos de RT-qPCR para a detecção do FMDV. Os métodos avaliados para a região 3D demonstraram sensibilidade e especificidade semelhantes. No entanto, foram observadas diferenças notáveis em amostras com baixa concentração de RNA. Embora esses métodos possam ser utilizados de forma intercambiável com resultados consistentes ao lidar com um grande número de amostras enviadas ao laboratório para identificar um surto, variações sutis podem assumir importância ao trabalhar com uma quantidade limitada de amostras contendo baixas cópias de RNA do FMDV. causador FMDV, FMDV , (FMDV) país RTqPCR RT qPCR D semelhantes entanto surto (FMDV

Abstract This study aimed to assess the influence of streptozotocin (STZ)-induced diabetes on the nociceptive behavior evoked by the injection of hypertonic saline (HS) into the masseter muscle of rats. Forty male rats were equally divided into four groups: a) isotonic saline control, which received 0.9% isotonic saline (IS), (Ctrl-IS); b) hypertonic saline control, which received 5% HS (Ctrl-HS); c) STZ-induced diabetic, which received IS, (STZ-IS); d) STZ-induced diabetic, which received HS (STZ-HS). Experimental diabetes was induced by a single intraperitoneal injection of STZ at dose of 60 mg/kg dissolved in 0.1 M citrate buffer, and 100 μL of HS or IS were injected into the left masseter to measure the nociceptive behavior. Later on, muscle RNA was extracted to measure the relative expression of the following cytokines: cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α), and interleukins (IL)-1β, -2, -6, and -10. One-way analysis of variance (ANOVA) was applied to the data (p < 0.050). We observed a main effect of group on the nociceptive response (ANOVA: F = 11.60, p < 0.001), where the Ctrl-HS group presented the highest response (p < 0.001). However, nociceptive response was similar among the Ctrl-IS, STZ-IS, and STZ-HS group (p > 0.050). In addition, the highest relative gene expression of TNF-α and IL-6 was found in the masseter of control rats following experimental muscle pain (p < 0.050). In conclusion, the loss of somatosensory function can be observed in deep orofacial tissues of STZ-induced diabetic rats. STZinduced (HS groups 09 0 9 0.9 , (IS) CtrlIS Ctrl (Ctrl-IS) b 5 CtrlHS (Ctrl-HS) c STZIS (STZ-IS) d STZHS. STZHS . (STZ-HS) 6 mgkg mg kg 01 1 0. buffer 10 cytokines cyclooxygenase2 cyclooxygenase 2 cyclooxygenase- COX2, COX2 COX (COX-2) TNFα, TNFα TNF α (TNF-α) IL1β, IL1β ILβ IL 1β, 1β β (IL)-1β 2, -2 6, -6 10. -10 Oneway One way ANOVA (ANOVA 0.050. 0050 0.050 050 0.050) 1160 11 11.60 0.001, 0001 0.001 001 0.001) 0.001. However CtrlIS, Ctrl-IS STZIS, STZ-IS addition IL6 IL- conclusion (IS (Ctrl-IS (Ctrl-HS (STZ-IS (STZ-HS (COX-2 (TNF-α - -1 005 0.05 05 116 11.6 000 0.00 00 (COX- 0.0 11. (COX

ABSTRACT Objective: To investigate associations/correlations between bone alterations and vitamin D status in children through data mining analyses based on observational studies. Material and Methods: Searches in PubMed, Scopus, Web of Science, and Embase databases were performed to recover studies, published until October 2022, with healthy children, which investigated the vitamin D status, related or not to undesirable bone alterations linked to bone quality (bone mineral density and bone mineral content), fracture or anthropometry. Country, study design, area of expertise (medicine, nutrition, dentistry, others), bone outcome, 25-hydroxyvitamin D data (serum or intake levels), the exams for bone diagnosis, and the results were analyzed in the VantagePointTM software. Results: Of 20,583 studies, 27 were included. The USA (n=9; 33.3%) had the highest number of publications. Cross-sectional (n=11; 40.7%), case-control (n=9; 33.3%), and cohort studies (n=7; 25.9%) contemplated the medicine and nutritional areas without any study in dentistry. Studies about bone quality (n=21; 77.8%), analyzed through dual-energy X-ray absorptiometry (DXA; n=14; 51.8%), with association (n=16; 59.2%) between the low serum levels of 25-hydroxyvitamin D and undesirable bone alterations (n=14; 51.8%) were the most prevalent. Conclusion: Most studies were conducted in the medical area and showed an association between low bone quality and low levels of 25-hydroxyvitamin D, verified through DXA. Objective associationscorrelations associations correlations Methods PubMed Scopus Science 2022 content, content , content) anthropometry Country design medicine, (medicine nutrition dentistry others, others others) outcome 25hydroxyvitamin hydroxyvitamin 25 levels, levels) diagnosis software Results 20583 20 583 20,58 2 included n=9 n9 n 9 (n=9 33.3% 333 33 3 publications Crosssectional Cross sectional n=11 n11 11 (n=11 40.7%, 407 40.7% 40 7 40.7%) casecontrol case control 33.3%, n=7 n7 (n=7 25.9% 259 n=21 n21 21 (n=21 77.8%, 778 77.8% 77 8 77.8%) dualenergy dual energy Xray X ray DXA (DXA n=14 n14 14 51.8%, 518 51.8% 51 n=16 n16 16 (n=16 59.2% 592 59 (n=14 prevalent Conclusion 202 2058 58 20,5 n= (n= 33.3 n=1 n1 1 (n=1 40.7 4 25.9 n=2 n2 (n=2 77.8 51.8 5 59.2 205 20, (n 33. 40. 25. 77. 51. 59.

Abstract To explore the diversity of scenarios in nature, animals have evolved tools to interact with different environmental conditions. Chemoreceptors are an important interface component and among them, olfactory receptors (ORs) and gustatory receptors (GRs) can be used to find food and detect healthy resources. Drosophila is a model organism in many scientific fields, in part due to the diversity of species and niches they occupy. The contrast between generalists and specialists Drosophila species provides an important model for studying the evolution of chemoreception. Here, we compare the repertoire of chemoreceptors of different species of Drosophila with that of D. incompta, a highly specialized species whose ecology is restricted to Cestrum flowers, after reporting the preferences of D. incompta to the odor of Cestrum flowers in olfactory tests. We found evidence that the chemoreceptor repertoire in D. incompta is smaller than that presented by species in the Sophophora subgenus. Similar patterns were found in other non-Sophophora species, suggesting the presence of underlying phylogenetic trends. Nevertheless, we also found autapomorphic gene losses and detected some genes that appear to be under positive selection in D. incompta, suggesting that the specific lifestyle of these flies may have shaped the evolution of individual genes in each of these gene families. nature conditions them ORs (ORs GRs (GRs resources fields occupy chemoreception Here D tests subgenus nonSophophora non trends Nevertheless families

Abstract Background Transcatheter aortic valve implantation (TAVI) has established itself as the preferential strategy to approach severe aortic stenosis. Information on procedural improvements and nationwide results obtained with the technique throughout the past decade are unknown. Objectives To assess the temporal variation of the demographic profile, procedural characteristics, and in-hospital outcomes of patients undergoing TAVI procedures at the Rede D’Or São Luiz. Methods Observational registry comprising 29 national institutions, comparing the characteristics of the TAVI procedures performed from 2012 to 2017 (Group 1) to those performed from 2018 to 2023 (Group 2). The statistical significance level adopted was p < 0.05. Results This study assessed 661 patients, 95 in Group 1 and 566 in Group 2, with a mean age of 81.1 years. Group 1 patients had a higher prevalence of New York Heart Association functional class III or IV and STS risk score > 8%. In addition, they more often underwent general anesthesia, transesophageal echocardiographic monitoring, and access through femoral dissection. Group 2 patients had a higher success rate of the TAVI procedure (95.4% versus 89.5%; p = 0.018), lower mortality (3.9% versus 11.6%; p = 0.004), and less often needed permanent pacemaker implantation (8.5% versus 17.9%; p = 0.008). Conclusions The 10-year temporal trends analysis of the TAVIDOR Registry shows a reduction in patients’ clinical complexity over time. Furthermore, the advance to minimalistic implantation techniques, added to the technological evolution of the devices, may have contributed to the favorable outcomes observed among those whose implantation occurred in the last 5 years studied. (TAVI stenosis unknown profile inhospital hospital DOr D Or Luiz institutions 201 202 2. . 2) 005 0 05 0.05 66 9 56 811 81 81. 8 8% addition anesthesia monitoring dissection 95.4% 954 4 (95.4 89.5% 895 89 0.018, 0018 0.018 , 018 0.018) 3.9% 39 3 (3.9 11.6% 116 11 6 0.004, 0004 0.004 004 0.004) 8.5% 85 (8.5 17.9% 179 17 0.008. 0008 0.008 008 0.008) 10year year 10 time Furthermore techniques devices studied 20 00 0.0 95.4 (95. 89.5 001 0.01 01 3.9 (3. 11.6 000 0.00 8.5 (8. 17.9 0. 95. (95 89. 3. (3 11. 8. (8 17. (9 (

Resumo Fundamento O implante percutâneo de bioprótese valvar aórtica (TAVI) consolidou-se como opção terapêutica da estenose aórtica de grau importante. Dados sobre as características evolutivas dos procedimentos e dos resultados obtidos com a técnica ao longo da última década, em escala nacional, são desconhecidos. Objetivos Analisar a tendência temporal referente ao perfil demográfico, características dos procedimentos e desfechos hospitalares de pacientes submetidos a TAVI na Rede D’Or São Luiz. Métodos Registro observacional envolvendo 29 instituições nacionais. Comparou-se características dos procedimentos realizados de 2012 a 2017 (Grupo 1) e de 2018 a 2023 (Grupo 2). Foram considerados significantes os resultados com valor de p < 0,05. Resultados Foram analisados 661 casos, 95 pertencentes ao Grupo 1 e 566 ao Grupo 2. A média de idade foi 81,1 anos. Observou-se no Grupo 1 maior prevalência de pacientes em classe funcional III ou IV e escore de risco > 8%. Foi mais frequente o emprego de anestesia geral, monitorização ecocardiográfica transesofágica e via de acesso por dissecção. Maior taxa de sucesso do procedimento (95,4% versus 89,5%; p = 0,018) foi aferida em implantes efetivados a partir de 2018, assim como menor mortalidade (3,9% versus 11,6%; p = 0,004) e necessidade de marcapasso definitivo (8,5% versus 17,9%; p = 0,008). Conclusões A análise temporal de 10 anos do Registro TAVIDOR demonstra uma queda na complexidade clínica dos pacientes. Além disso, o avanço para técnicas de implante minimalistas, somadas à evolução tecnológica dos dispositivos, podem ter contribuído para desfechos favoráveis dentre aqueles cujo implante ocorreu no último quinquênio. (TAVI consolidouse consolidou se importante década nacional desconhecidos demográfico DOr D Or Luiz 2 nacionais Comparouse Comparou 201 202 . 2) 005 0 05 0,05 66 casos 9 56 811 81 81, Observouse Observou 8 8% geral dissecção 95,4% 954 4 (95,4 89,5% 895 89 5 0,018 0018 018 3,9% 39 3 (3,9 11,6% 116 11 6 0,004 0004 004 8,5% 85 (8,5 17,9% 179 17 0,008. 0008 0,008 008 0,008) disso minimalistas dispositivos quinquênio 20 00 0,0 95,4 (95, 89,5 0,01 001 01 3,9 (3, 11,6 0,00 000 8,5 (8, 17,9 0, 95, (95 89, 3, (3 11, 8, (8 17, (9 (

ABSTRACT Exacerbated inflammation and coagulation are a hallmark of COVID-19 severity. Extracellular vesicles (EVs) are intercellular transmitters involved in inflammatory conditions, which are capable of triggering prothrombotic mechanisms. Since the release of EVs is potentially associated with COVID-19-induced coagulopathy, the aim of this study was to evaluate changes in inflammation- and hypercoagulability-related EVs during the first month after symptom onset and to determine whether they are associated with disease severity. Blood samples of patients with mild or severe forms of the disease were collected on three occasions: in the second, third and fourth weeks after symptom onset for the quantification by flow cytometry of CD41A (platelet glycoprotein IIb/IIIa), CD162 (PSGL-1), CD31 (PECAM-1) and CD142 cells (tissue factor). Analysis of variance (ANOVA) with repeated measures, Kruskal-Wallis and correlation tests were used. Eighty-five patients were enrolled, 71% of whom had mild disease. Seventeen uninfected individuals served as controls. Compared to controls, both mild and severe COVID-19 were associated with higher EV-CD31+, EV-CD41+ and EV-CD142+ levels. All EV levels were higher in severe than in mild COVID-19 only after the third week from symptom onset, as opposed to C-reactive protein and D-dimer levels, which were higher in severe than in mild COVID-19 earlier during disease progression. EV levels were also associated with C-reactive protein and D-dimer levels only after the third week of symptoms. In conclusion, EVs expressing CD41A, CD31, TF, and CD162 appear as late markers of COVID-19 severity. This finding may contribute to the understanding of the pathogenesis of acute and possibly long COVID-19. COVID19 COVID 19 COVID-1 severity (EVs conditions mechanisms COVID19induced COVIDinduced induced coagulopathy hypercoagulabilityrelated hypercoagulability related occasions second CDA CD A platelet IIb/IIIa, IIbIIIa IIb/IIIa , IIb IIIa IIb/IIIa) CD16 PSGL1, PSGL1 PSGL 1 (PSGL-1) CD3 PECAM1 PECAM (PECAM-1 CD14 tissue factor. factor . factor) ANOVA (ANOVA measures KruskalWallis Kruskal Wallis used Eightyfive Eighty five enrolled 71 controls EVCD31+, EVCD31 EVCD CD31+, EV-CD31+ EVCD41+ EVCD41 CD41+ CD41 EV-CD41 EVCD142+ EVCD142 CD142+ EV-CD142 Creactive C reactive Ddimer D dimer progression symptoms conclusion TF COVID19. 19. COVID1 COVID- CD1 (PSGL-1 (PECAM- 7 EVCD31+ EVCD3 CD31+ EV-CD31 EVCD4 CD4 EV-CD4 EVCD14 EV-CD14 (PSGL- (PECAM EV-CD3 EV-CD EVCD1 EV-CD1 (PSGL

ABSTRACT The objective of this study was to evaluate the effects of an essential oil blend (EO), based on cinnamaldehyde and diallyl disulfide, associated or not with antibiotics on intake and nutrient digestibility, ingestive behavior, rumen fermentation, ruminal microbial synthesis, and blood metabolites of feedlot cattle fed a no-forage diet. The study was carried out as a Latin Square with five treatments consisting of a blend of essential oil (EO), monensin (MON) and virginiamycin (VM), both separately and combined as follows: CON (monensin at 30 mg kg–1 DM), VM (virginiamycin at 25 mg kg–1 DM and monensin at 30 mg kg–1 DM), MEO25 (monensin at 30 mg kg–1 DM and EO at 25 mg kg–1 DM), MEO35 (monensin at 30 mg kg–1 DM and EO at 35 mg kg–1 DM) and EO35 (blend of EO at 35 mg kg–1 DM). There were no effects from additives and their combinations on the intake and apparent digestibility of nutrients or ingestive behavior. Furthermore, treatments did not modify the ruminal pH, nor the concentration of short-chain fatty acids and ammonia, nor the microbial protein synthesis. Blood glucose concentration was higher 4 h after morning feeding for all treatments. There was a significant contrast between the VM and EO for the blood concentration of D-Lactate and L-Lactate. There was no difference between the additives in the concentration of cortisol metabolites in the feces. The blend of essential oil studied, containing cinnamaldehyde and diallyl disulfide, associated or not with antibiotics, does not change the nutritional parameters nor the metabolism of feedlot cattle fed a no-forage diet. EO, , (EO) disulfide behavior fermentation synthesis noforage forage diet MON (MON VM, (VM) follows 3 kg1 kg 1 kg– DM, 2 MEO MEO2 MEO3 EO3 DM. . Furthermore pH shortchain short chain ammonia DLactate D Lactate LLactate. LLactate L Lactate. L-Lactate feces studied (EO (VM

Resumo: O raciocínio e a emoção apresentam complexas e numerosas relações entre si, podendo prejudicar ou beneficiar o processamento lógico. Visando investigar a influência da tonalidade afetiva sobre o raciocínio, realizou-se uma revisão sistemática advinda da busca de estudos publicados nas bases de dados do Portal de Periódicos da Capes, Scielo, PubMed e Google Acadêmico. Os descritores utilizados foram: (affective content OR evaluative content OR emotion*) AND (reasoning). Após análise e submissão aos critérios de inclusão e exclusão, restaram 17 artigos, duas dissertações de mestrado e uma tese de doutorado. Apenas um dos estudos foi realizado no Brasil. Os participantes das pesquisas selecionadas tiveram pior desempenho nas tarefas de raciocínio formadas por conteúdos valorativos negativos. Entretanto, os conteúdos negativos influenciaram de forma positiva o raciocínio dedutivo quando se mostravam relevantes para a realização da tarefa. Assim, os estudos selecionados parecem apontar para a importância das emoções sobre o raciocínio humano. Resumo si lógico realizouse realizou Capes Scielo Acadêmico foram affective emotion* emotion reasoning. reasoning . (reasoning) exclusão 1 artigos doutorado Brasil Entretanto tarefa Assim humano (reasoning

Resumen: El razonamiento y las emociones tienen relaciones complejas entre sí, que pueden perjudicar o ayudar el procesamiento lógico. Para investigar la influencia del tono afectivo sobre el razonamiento, se realizó una revisión sistemática a partir de la búsqueda de estudios publicados en las bases de datos del Portal de Periódicos Capes, SciELO, PubMed y Google Académico. Los descriptores utilizados fueron los siguientes: (affective content OR evaluative content OR emotion*) AND (reasoning). Después de la aplicación de los criterios de inclusión y exclusión, se seleccionaron 17 artículos, dos disertaciones de maestría y una tesis doctoral. Solo uno de los estudios se llevó a cabo en Brasil. Los participantes de las investigaciones seleccionadas tuvieron un peor desempeño en las tareas de razonamiento formadas por contenidos emocionales negativos. Estos influenciaron de forma positiva el razonamiento deductivo cuando eran relevantes para la realización de la tarea. Los estudios seleccionados parecen evidenciar la importancia de las emociones sobre el razonamiento humano. Resumen sí lógico Capes SciELO Académico siguientes affective emotion* emotion reasoning. reasoning . (reasoning) exclusión 1 artículos doctoral Brasil negativos tarea humano (reasoning

Résumé : Le raisonnement et l’émotion entretiennent de nombreuses relations complexes qui peuvent entraver ou favoriser le procès logique. Dans le but d’étudier l’influence du contenu affectif sur le raisonnement, on a effectué une revue systématique des articles scientifiques publiés sur les base des données Portail des Périodiques du Capes, Scielo, PubMed et Google Académique, retrouvés en utilisant les descripteurs (affective content OR evaluative content OR emotion*) AND (reasoning). Après l’analyse et application des critères d’inclusion et d’exclusion, 17 articles, deux mémoires de maîtrise et une thèse de doctorat ont été retenus. Une seule étude a été menée au Brésil. Les participants aux recherches ont montré une pire perfomance dans les tâches de raisonnement composés d’un contenu affectif négatif. Cependant, ce contenu a influencé positivement le raisonnement déductif lorsqu’il s’est avéré pertinent pour la tâche. Les études examinées suggèrent donc que les émotions jouent un rôle important dans le raisonnement humain. lémotion l émotion logique détudier d étudier linfluence influence Capes Scielo Académique affective emotion* emotion reasoning. reasoning . (reasoning) lanalyse analyse dinclusion inclusion dexclusion, dexclusion exclusion, exclusion d’exclusion 1 retenus Brésil dun négatif Cependant lorsquil lorsqu il sest s est tâche humain (reasoning

Abstract: Reasoning and emotions have many complex relations which can hinder or benefit logical processing. Intending to investigate the influence of affective content on reasoning, we conducted a systematic review on scientific articles published on the Capes Journals Portal, Scielo, PubMed and Academic Google databases, retrieved using the descriptors (affective content OR evaluative content OR emotion*) AND (reasoning). After analysis and application of inclusion and exclusion criteria, 17 papers, two master’s dissertations and one doctorate thesis remained. Only one study was conducted in Brazil. Research participants showed a worse performance in reasoning tasks composed by negative emotional content. However, negative emotional content influenced deductive reasoning positively when they proved to be relevant to the task. Hence, the reviewed studies suggest an importance of emotions for human reasoning. Abstract processing Portal Scielo databases emotion* emotion . (reasoning) criteria 1 papers masters master s remained Brazil However task Hence (reasoning

Resumo Este estudo transversal teve como objetivo investigar a associação entre defeitos de desenvolvimento do esmalte (DDE) e polimorfismos de nucleotídeo único (SNPs) nos genes que codificam o receptor da vitamina D (VDR) e o hormônio da paratireoide (PTH). Pacientes ortodônticos em tratamento em uma escola Odontologia foram selecionados por amostragem de conveniência. Os DDEs foram avaliados e classificados por um examinador calibrado (Kappa>0,80) através de fotografias intraorais de acordo com os critérios propostos por Ghanim et al. (2015). Os tipos de DDE considerados para análise foram: hipoplasia de esmalte, hipomineralização molar-incisivo (HMI), hipomineralização de segundos molares decíduos (HSMD) e opacidades demarcadas não-HMI/HSMD. O DNA gnômico foi extraído de células bucais. Os SNPs em VDR (rs7975232) e PTH (rs694, rs6256 e rs307247) foram genotipados por PCR em tempo real. As análises estatísticas foram realizadas utilizando o software PLINK (versão 1.03, concebido por Shaun Purcell, EUA). Foram feitos teste de qui-quadrado e teste exato de Fisher com um nível de significância de 5%. Foram incluídos noventa e um (n=91) pacientes (49 do sexo feminino e 42 do sexo masculino) (idade média de 14,1±5,8 anos). A frequência de DDE foi de 38,5% (35 pacientes). As distribuições genotípicas estavam em equilíbrio de Hardy-Weinberg. Não foi encontrada associação estatisticamente significante entre os DDEs e os SNPs avaliados. Foi observada uma associação limítrofe (p=0,09) entre a DDE e o haplótipo CC para o SNP rs7975232 no VDR. Em conclusão, os SNPs seleccionados nos genes VDR e PTH não foram associados à DDE nas amostras estudadas. (DDE (SNPs (VDR PTH. . (PTH) conveniência Kappa>0,80 Kappa080 Kappa 0 80 (Kappa>0,80 al 2015. 2015 (2015) molarincisivo molar incisivo HMI, HMI , (HMI) HSMD (HSMD nãoHMI/HSMD. nãoHMIHSMD HMI/HSMD. não-HMI/HSMD bucais rs (rs7975232 rs694, rs694 (rs694 rs625 rs307247 real versão 103 1 03 1.03 Purcell EUA. EUA EUA) quiquadrado qui quadrado 5 5% n=91 n91 n 91 (n=91 49 (4 4 masculino idade 14158 14 8 14,1±5, anos. anos anos) 385 38 38,5 35 (3 pacientes. pacientes) HardyWeinberg. HardyWeinberg Hardy Weinberg. Weinberg Hardy-Weinberg p=0,09 p009 p 09 (p=0,09 rs797523 conclusão estudadas (PTH Kappa>0,8 Kappa08 (Kappa>0,8 201 (2015 (HMI nãoHMI nãoHMI/HSMD HMIHSMD HMI/HSMD (rs797523 rs69 (rs69 rs62 rs30724 10 1.0 n=9 n9 9 (n=9 ( 1415 14,1±5 3 38, p=0,0 p00 (p=0,0 rs79752 Kappa>0, Kappa0 (Kappa>0, 20 (201 (rs79752 rs6 (rs6 rs3072 1. n= (n= 141 14,1± p=0, p0 (p=0, rs7975 Kappa>0 (Kappa>0 2 (20 (rs7975 (rs rs307 (n 14,1 p=0 (p=0 rs797 Kappa> (Kappa> (2 (rs797 rs30 14, p= (p= rs79 (Kappa (rs79 rs3 (p rs7 (rs7

Abstract This cross-sectional study aimed to investigate the association between developmental defects of enamel (DDE) and single nucleotide polymorphisms (SNPs) in the genes encoding the vitamin D receptor (VDR) and parathyroid hormone (PTH). Orthodontic patients receiving treatment at a dental school were selected through convenience sampling. Intra-oral photographs were used to assess DDE, which were classified according to the criteria proposed by Ghanim et al. (2015) by a single calibrated examiner (Kappa>0.80). Enamel hypoplasia, molar-incisor hypomineralization (MIH), hypomimineralized second primary molar (HSPM), and non-MIH/HSPM demarcated opacities were considered for the analysis. Genomic DNA was extracted from buccal cells. The SNPs in VDR (rs7975232) and PHT (rs694, rs6256, and rs307247) were genotyped using real-time polymerase chain reactions (PCR). Statistical analyses were performed using the PLINK software (version 1.03, designed by Shaun Purcell, EUA). Chi-square or Fisher's exact tests were performed at a significance level of 5%. Ninety-one (n=91) patients (49 females and 42 males) (mean age of 14.1±5.8 years) were included. The frequency of DDE was 38.5% (35 patients). Genotype distributions were in Hardy-Weinberg equilibrium. No significant statistical association was found between DDE and the SNPs evaluated. A borderline association (p=0.09) was observed between DDE and the CC haplotype for SNP rs7975232 in VDR. In conclusion, the selected SNPs in VDR and PTH genes were not associated with DDE in the studied samples. crosssectional cross sectional (DDE (SNPs (VDR PTH. . (PTH) sampling Intraoral Intra oral al 2015 (2015 Kappa>0.80. Kappa080 Kappa Kappa>0.80 0 80 (Kappa>0.80) hypoplasia molarincisor incisor MIH, MIH , (MIH) HSPM, HSPM (HSPM) nonMIH/HSPM nonMIHHSPM non MIH/HSPM analysis cells rs (rs7975232 rs694, rs694 (rs694 rs6256 rs307247 realtime real time PCR. PCR (PCR) version 103 1 03 1.03 Purcell EUA. EUA EUA) Chisquare Chi square Fishers Fisher s 5 5% Ninetyone Ninety one n=91 n91 n 91 (n=91 49 (4 4 males mean 14158 14 8 14.1±5. years included 385 38 38.5 35 (3 patients. patients) HardyWeinberg Hardy Weinberg equilibrium evaluated p=0.09 p009 p 09 (p=0.09 rs797523 conclusion samples (PTH 201 (201 Kappa08 Kappa>0.8 (Kappa>0.80 (MIH (HSPM nonMIH MIHHSPM (rs797523 rs69 (rs69 rs625 rs30724 (PCR 10 1.0 n=9 n9 9 (n=9 ( 1415 14.1±5 3 38. p=0.0 p00 (p=0.0 rs79752 20 (20 Kappa0 Kappa>0. (Kappa>0.8 (rs79752 rs6 (rs6 rs62 rs3072 1. n= (n= 141 14.1± p=0. p0 (p=0. rs7975 2 (2 Kappa>0 (Kappa>0. (rs7975 (rs rs307 (n 14.1 p=0 (p=0 rs797 Kappa> (Kappa>0 (rs797 rs30 14. p= (p= rs79 (Kappa> (rs79 rs3 (p rs7 (Kappa (rs7

Resumo Objetivo Neste estudo, investigamos o impacto de uma intrusão marinha nas características da coluna d'água da lagoa de Jurubatiba, um ecossistema costeiro tropical, por 12 meses após um evento de abertura da barra de areia, a fim de elucidar a duração de seus efeitos na lagoa. Métodos Amostramos mensalmente as características físicas, químicas e biológicas ao longo do perfil vertical da coluna d'água após a abertura da barra de areia e entrada de água do mar. Avaliamos a associação temporal (mensalmente) e espacial (ao longo do perfil de profundidade) entre essas variáveis e a correlação temporal na heterogeneidade vertical dessas variáveis e condições ambientais que promovem a estratificação. Resultados A intrusão marinha resultou na formação de cunhas salinas em curto prazo, levando à estratificação na concentração de oxigênio dissolvido, pH, salinidade e clorofila-a ao longo da coluna de água por, pelo menos, quatro meses. No entanto, a temperatura atmosférica e a incidência solar também emergiram como fatores cruciais associados à heterogeneidade vertical nas variáveis limnológicas, influenciando a densidade da água e o pH ao longo do período de estudo. A precipitação e a velocidade do vento tiveram apenas efeitos marginais nas variações térmicas e ectogênicas, provavelmente mediadas pela cor da água, entrada de nutrientes, profundidade da coluna d'água e orientação da lagoa na paisagem. Conclusões Apesar de rasas e expostas a ação dos ventos, as lagoas costeiras podem apresentar uma considerável heterogeneidade vertical nas condições limnológicas devido a vários fatores climáticos e hidrodinâmicos. Notavelmente, os efeitos de intrusões marinhas na salinidade e na estratificação da coluna d'água podem persistir por vários meses, afetando a qualidade da água e, provavelmente, as comunidades bióticas e o funcionamento do ecossistema. Uma vez que as atividades humanas e as mudanças climáticas podem intensificar as intrusões marinhas em lagoas costeiras, compreender seus efeitos na dinâmica limnológica é fundamental para nossa capacidade de prevê-los e conservar ecossistemas tão vulneráveis. estudo dágua d Jurubatiba tropical 1 físicas mar (mensalmente prazo dissolvido clorofilaa clorofila menos entanto ectogênicas nutrientes paisagem ventos hidrodinâmicos Notavelmente prevêlos prevê los vulneráveis

Abstract Aim In this study, we investigated the impact of an episodic marine intrusion on the water column characteristics of Jurubatiba lagoon, a tropical coastal ecosystem, for 12 months following a sandbar breaching event to elucidate the duration of its effects. Methods Monthly, we sampled the physical, chemical, and biological characteristics along the water column for one year after a sandbar breaching event and seawater inflow. We assessed the temporal (monthly) and spatial (along depth profiles) associations among these variables and the temporal correlation among vertical heterogeneities in these variables and environmental drivers of stratification. Results Marine intrusion resulted in salt wedges formation in the short-term, leading to stratification in dissolved oxygen, pH, salinity, and chlorophyll-a concentration along the water column for at least four months. However, temperature and solar incidence also emerged as crucial factors associated with vertical heterogeneity in limnological variables, influencing water density and pH throughout the study period. Precipitation and wind speed had only marginal effects on thermal and ectogenic stratifications, probably mediated by water colour, nutrient inputs, water column depth and lagoon’s orientation in the landscape. Conclusions Our data unveiled that, despite their shallowness and wind exposure, coastal lagoons can exhibit considerable vertical heterogeneities in limnological variables due to various climatic and hydrodynamic aspects. Notably, the effects of marine intrusions on salinity and ectogenic column stratification can persist for several months, affecting water quality and, probably, biotic communities and ecosystem functioning. Once human activities and climate change may intensify marine intrusions on coastal lagoons, understanding spatiotemporal dynamics and their drivers is fundamental to anticipating their effects and conserving such vulnerable ecosystems. lagoon 1 Monthly physical chemical inflow monthly (monthly profiles shortterm, shortterm short term, term short-term oxygen chlorophylla chlorophyll However period stratifications colour inputs s landscape that exposure aspects Notably functioning ecosystems

Resumo: Este artigo faz uma análise genealógica da inclusão da “agressividade infantil” e suas variações no Manual Diagnóstico e Estatístico de Transtornos Mentais (DSM) ao longo das suas edições. Começamos por avaliar a relevância da inclusão deste signo clínico nos critérios de diagnóstico, sobretudo a partir de 1980. Em seguida, analisamos as implicações clínicas e políticas desta inclusão, em particular no que concerne à construção de uma psicopatologia da infância e à definição social de normas de conduta. Por fim, analisamos como a ênfase colocada na normatização da agressividade infantil através de discursos normativos, em consonância com as exigências neoliberais de desempenho, contribui para o aumento da expressão da agressividade como forma de lidar com o mal-estar e com o sofrimento subjetivo. Resumo DSM (DSM edições diagnóstico 1980 seguida conduta fim normativos desempenho malestar mal estar subjetivo 198 19 1

Abstract: This article provides a genealogical analysis of the inclusion of “child aggressiveness” and its variations in the Diagnostic and Statistical Manual of Mental Disorders (DSM) throughout its editions. We begin by assessing the relevance of the inclusion of this clinical sign in the diagnostic criteria, especially since 1980. We then analyze the clinical and political implications of this inclusion, particularly regarding the construction of a psychopathology of childhood and the social definition of norms of conduct. Finally, we analyse how the emphasis placed on the normalization of child aggression through normative discourses, in line with neoliberal demands for performance, contributes to an increase in the expression of aggression as a way of dealing with malaise and subjective suffering. Abstract aggressiveness DSM (DSM editions criteria 1980 conduct Finally discourses performance suffering 198 19 1

Resumen: Este artículo realiza un análisis genealógico de la inclusión de la “agresividad infantil” y sus variaciones en el Manual Diagnóstico y Estadístico de los Trastornos Mentales (DSM) a lo largo de sus ediciones. Comenzamos evaluando la relevancia de la inclusión de este signo clínico en los criterios diagnósticos, especialmente desde 1980. A continuación, analizamos las implicaciones clínicas y políticas de esta inclusión, sobre todo en lo que respecta a la construcción de una psicopatología de la infancia y a la definición social de las normas de comportamiento. Por último, analizamos cómo el énfasis puesto en la normalización de la agresividad infantil a través de discursos normativos, en consonancia con las exigencias neoliberales de rendimiento, contribuye a un aumento de la expresión de la agresividad como forma de afrontar el malestar y el sufrimiento subjetivo. Resumen DSM (DSM ediciones diagnósticos 1980 continuación comportamiento último normativos rendimiento subjetivo 198 19 1

Résumé : Cet article propose une analyse généalogique de l’inclusion de l’« agressivité infantile » et de ses variations dans le Manuel diagnostique et statistique des troubles mentaux (DSM) au fil de ses éditions. Nous commençons par évaluer la pertinence de l’inclusion de ce signe clinique dans les critères diagnostiques, en particulier depuis 1980. Nous analysons ensuite les implications cliniques et politiques de cette inclusion, notamment en ce qui concerne la construction d’une psychopathologie de l’enfance et la définition sociale de normes comportementales. Enfin, nous analysons comment l’accent mis sur la normalisation de l’agressivité de l’enfant par les discours normatifs, en accord avec les exigences de performance néolibérales, contribue à l’augmentation de l’expression de l’agressivité comme moyen de faire face au malaise et à la souffrance subjective. linclusion l inclusion l« « l’ DSM (DSM éditions diagnostiques 1980 dune d lenfance enfance comportementales Enfin laccent accent lagressivité lenfant enfant normatifs néolibérales laugmentation augmentation lexpression expression subjective 198 19 1