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Resumo A avaliação do atraso de colheita de sementes de soja pode auxiliar na seleção de cultivares mais resistentes à deterioração no campo. O objetivo deste trabalho foi avaliar a qualidade fisiológica e sanitária de sementes de três cultivares de soja, submetidas ao atraso da colheita. Sementes das cultivares ‘NS 5959’, ‘BMX Potência’ e ‘TMG 1175’ foram colhidas em quatro épocas (R8; R8 + 10 dias; R8 + 20 dias e R8 + 30 dias) e avaliadas quanto ao grau de umidade, germinação, emergência, crescimento de plântulas e incidência de fungos. Houve redução na germinação e vigor das sementes das diferentes cultivares de soja, principalmente quando colhidas em R8 + 20 e R8 + 30 dias, devido à deterioração em campo. Além disso, o atraso na colheita proporcionou maior incidência de fungos nas sementes, principalmente Phomopsis sp., Aspergillus sp. e Cercospora kikuchii. Em geral, a cultivar ‘TMG 1175’ foi mais resistente à deterioração das sementes em campo causada pelo atraso de colheita.

Abstract The assessment of delayed harvesting of soybean seeds may help in the selection of cultivars more resistant to deterioration in the field. This work aimed to evaluate the physiological and sanitary quality of seeds of three soybean cultivars submitted to harvest delay. Seeds of the cultivars ‘NS 5959’, ‘BMX Potência’, and ‘TMG 1175’ were harvested in four periods (R8; R8 + 10 days; R8 + 20 days, and R8 + 30 days) and evaluated for the moisture content, germination, emergence, seedling growth, and fungal incidence. There was a reduction in germination and vigor of the seeds of the different soybean cultivars, especially when harvested at R8 + 20 and R8 + 30 days, due to deterioration in the field. In addition, the delay in harvest provided a higher incidence of fungi in the seeds, mainly Phomopsis sp., Aspergillus sp., and Cercospora kikuchii. In general, the cultivar ‘TMG 1175’ was more resistant to seed deterioration in the field caused by delayed harvesting.

https://doi.org/10.19084/rca.31737

Posicionamento do Departamento de Imagem Cardiovascular da Sociedade Brasileira de Cardiologia sobre o Uso do Strain Miocárdico na Rotina do Cardiologista – 2023
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Almeida, André Luiz Cerqueira

; Melo, Marcelo Dantas Tavares de

; Bihan, David Costa de Souza Le

; Vieira, Marcelo Luiz Campos

; Pena, José Luiz Barros

; Del Castillo, José Maria

; Abensur, Henry

; Hortegal, Renato de Aguiar

; Otto, Maria Estefania Bosco

; Piveta, Rafael Bonafim

; Dantas, Maria Rosa

; Assef, Jorge Eduardo

; Beck, Adenalva Lima de Souza

; Santo, Thais Harada Campos Espirito

; Silva, Tonnison de Oliveira

; Salemi, Vera Maria Cury

; Rocon, Camila

; Lima, Márcio Silva Miguel

; Barberato, Silvio Henrique

; Rodrigues, Ana Clara

; Rabschkowisky, Arnaldo

; Frota, Daniela do Carmo Rassi

; Gripp, Eliza de Almeida

; Barretto, Rodrigo Bellio de Mattos

; Silva, Sandra Marques e

; Cauduro, Sanderson Antonio

; Pinheiro, Aurélio Carvalho

; Araujo, Salustiano Pereira de

; Tressino, Cintia Galhardo

; Silva, Carlos Eduardo Suaide

; Monaco, Claudia Gianini

; Paiva, Marcelo Goulart

; Fisher, Cláudio Henrique

; Alves, Marco Stephan Lofrano

; Grau, Cláudia R. Pinheiro de Castro

; Santos, Maria Veronica Camara dos

; Guimarães, Isabel Cristina Britto

; Morhy, Samira Saady

; Leal, Gabriela Nunes

; Soares, Andressa Mussi

; Cruz, Cecilia Beatriz Bittencourt Viana

; Guimarães Filho, Fabio Villaça

; Assunção, Bruna Morhy Borges Leal

; Fernandes, Rafael Modesto

; Saraiva, Roberto Magalhães

; Tsutsui, Jeane Mike

; Soares, Fábio Luis de Jesus

; Falcão, Sandra Nívea dos Reis Saraiva

; Hotta, Viviane Tiemi

; Armstrong, Anderson da Costa

; Hygidio, Daniel de Andrade

; Miglioranza, Marcelo Haertel

; Camarozano, Ana Cristina

; Lopes, Marly Maria Uellendahl

; Cerci, Rodrigo Julio

; Siqueira, Maria Eduarda Menezes de

; Torreão, Jorge Andion

; Rochitte, Carlos Eduardo

; Felix, Alex

Arquivos Brasileiros de Cardiologia

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Dez 2023, Volume 120 Nº 12 elocation e20230646

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

https://doi.org/10.36660/abc.20230646

Performance Analysis of a Distributed Optical Fiber Vibration Sensor Based on a Sagnac Interferometer
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Ozcáriz, Aritz

; Weber, Guilherme Heim

; Gomes, Danilo Fernandes

; Costa, Igor Brutkowski Vieira da

; Galvão, José Rodolfo

; Martins, Victor Matheus

; Pipa, Daniel R.

; Silva, Marco J.

; Silva, Jean Carlos Cardozo da

; Zamarreño, Carlos Ruiz

; Martelli, Cicero

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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Mar 2023, Volume 22 Nº 1 Páginas 77 - 90

Resumo: En | Texto: En | PDF: En

Abstract In this work, measuring capabilities of a Sagnac Interferometer-based Distributed Optical Fiber Sensor (DOFS) system are demonstrated by comparing its results to a conventional amplitude-based Distributed Acoustic Sensing (DAS) system. Through a set of three different experiments, it was demonstrated that both sensing systems can detect and locate dynamical deformation along the fiber, in addition to determining its frequency components. The coherent amplitude-based DAS presented a lower background noise. In contrast, even though the Sagnac interferometry-based system presented low-frequency noise in the measurements, it is less susceptible to harmonic distortion for higher amplitude deformations since its sensing principles rely on the measurement of the accumulated phase rather than the amplitude of the backscattered signal purely. Accordingly, the Sagnac interferometry-based system arises as a promising and rather accessible topology for a DOFS implementation. work Interferometerbased Interferometer based (DOFS amplitudebased (DAS experiments fiber components contrast interferometrybased interferometry lowfrequency low measurements purely Accordingly implementation

https://doi.org/10.1590/2179-10742023v22i1265391

Osmopriming with selenium: physical and physiological quality of tomato seeds in response to water deficit
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Silveira, Alice de Souza

; Pinheiro, Daniel Teixeira

; Oliveira, Rafael Macedo de

; Dias, Denise Cunha Fernandes dos Santos

; Silva, Laércio Junio da

Journal of Seed Science

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2023, Volume 45 elocation e202345012

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract: Tomato is a crop sensitive to water deficit and the responses of seeds to germination under these conditions involve biochemical, physiological, and molecular processes. The aim of this study was to evaluate the physical, physiological, and biochemical changes in tomato seeds osmoprimed with selenium (Se) and subjected to water deficit during germination. Tomato seeds of the LA 4050 and LA 3475 accessions were osmoprimed with PEG 6000 solution (-1.0 MPa) plus Na2SeO4 (12.5 μM) or only with PEG 6000 solution for 24 h or unprimed. The seeds from the different treatments were subjected to water deficit (-0.3 MPa) or to control (0 MPa). Image analyses were performed (X-ray), physiological variables were obtained by the germination test and activities of SOD, CAT and POX enzymes were determined. Osmopriming, especially with PEG, provided the seeds of both accessions with higher densities and gray mean when compared to unprimed ones. Seeds under water deficit showed lower physiological performance and lower enzymatic activity. Se did not induce seed resistance to water deficit. Osmopriming with PEG 6000 at -1.0 MPa is an efficient technique for improving the physiological quality of seeds of tomato accessions susceptible and tolerant to water deficit. Moreover, the antioxidant enzyme CAT proved to be an indicator of oxidative stress tolerance in tomato seedlings under water deficit.

Resumo: O tomate é uma cultura sensível ao déficit hídrico e as respostas de sementes à germinação sob essas condições envolvem processos bioquímicos, fisiológicos e moleculares. O objetivo do trabalho foi avaliar as alterações físicas, fisiológicas e bioquímicas em sementes de tomate osmocondicionadas com selênio (Se) e, posteriormente, submetidas ao déficit hídrico durante a germinação. Sementes de tomate das linhagens LA 4050 e LA 3475 foram osmocondicionadas com solução PEG 6000 (-1,0 MPa) mais Na2SeO4 (12,5 μM) ou apenas com solução de PEG 6000 por 24 h ou não condicionadas. As sementes provenientes dos diferentes tratamentos foram submetidas ao déficit hídrico (-0,3 MPa) ou ao controle (0 MPa). Foram realizadas análises de imagens (raios X); obtenção de variáveis fisiológicas pelo teste de germinação e atividades das enzimas SOD, CAT e POX. O osmocondicionamento, sobretudo com PEG, proporcionou às sementes de ambas as linhagens maiores densidades e média de cinza quando comparadas às não condicionadas. As sementes sob déficit hídrico apresentaram menor desempenho fisiológico e atividade enzimática. O Se não induziu resistência às sementes ao déficit hídrico. O osmocondicionamento com PEG 6000 a -1,0 MPa é eficiente para melhoria da qualidade fisiológica de sementes dos acessos de tomate suscetíveis e tolerantes ao déficit hídrico. Além disso, a enzima antioxidante CAT se mostrou indicadora da tolerância ao estresse oxidativo em plântulas de tomate sob déficit hídrico.

https://doi.org/10.1590/2317-1545v45267349

Study of physiological maturity of melon seeds by enzymatic changes
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Cassiano, Cristiano Vasconcelos

; Silva, Patricia Pereira da

; Pinheiro, Daniel Teixeira

; Dias, Denise Cunha Fernandes dos Santos

; Morais, Alexandre Augusto de

; Nascimento, Warley Marcos

Revista Ciência Agronômica

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2023, Volume 54 elocation e20207665

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Plant species have different adaptation mechanisms regarding seed survival to desiccation, preventing cellular destruction during the water loss. The knowledge of these mechanisms is of great importance for the understanding of how the seed formation/maturation processes and the processes involved in germination occur. Thus, this study aimed to determine physiological and enzymatic changes during the maturation process of melon seeds of the cultivar BRS Anton, obtained from fruits at different maturation stages and subjected to post-harvest storage, by the action of total proteins and the enzymes catalase, peroxidase, and superoxide dismutase. Melon fruits were grown in a greenhouse at Embrapa Vegetables and harvested at five different times: 30, 45, 60, 75, and 90 days after anthesis (DAA). Thirty fruits were harvested in each season and 15 fruits had their seeds extracted immediately after harvest and the other 15 fruits were stored for 15 days. After processing and drying, the seeds were submitted to the following analyses: germination, first count, protein profile analysis, and activity of antioxidant enzymes (total proteins, peroxidase, catalase, and superoxide dismutase). The activity of the antioxidant enzymes from 60 DAA under storage showed a more orderly behavior, reaffirming the results of physiological tests. It shows that the seeds at this point of physiological maturation are well-formed and drying caused no damage to their cell membranes.

RESUMO As espécies vegetais apresentam diferentes mecanismos de adaptação no que diz respeito à sobrevivência das sementes à dessecação, prevenindo a destruição celular durante a perda de água. O conhecimento desses mecanismos é de grande importância para o entendimento de como ocorre os processos de formação/maturação de sementes e dos processos envolvidos na germinação. Dessa forma, o objetivo deste trabalho foi determinar as alterações fisiológicas e enzimáticas durante o processo de maturação das sementes de melão da cultivar “BRS Anton”, obtidas de frutos em diferentes estádios de maturação e submetidas ao armazenamento pós-colheita, por meio da ação das proteínas totais e das enzimas catalase, peroxidase e superóxido dismutase. Os frutos de melão foram cultivados em casa de vegetação na Embrapa Hortaliças e colhidos em cinco épocas distintas: aos 30, 45, 60, 75 e 90 dias após a antese (DAA). Em cada época, foram colhidos 30 frutos, sendo que 15 frutos tiveram suas sementes extraídas imediatamente após a colheita e 15 frutos foram armazenados por 15 dias. Após o beneficiamento e secagem, as sementes foram submetidas às seguintes análises: germinação, primeira contagem, análise do perfil de proteínas e atividade de enzimas antioxidantes (proteínas totais, peroxidase, catalase e superóxido dismutase). Observou-se que as atividades das enzimas antioxidativas a partir dos 60 DAA sob armazenamento, apresentou um comportamento mais ordenado reafirmando os resultados dos testes fisiológicos. Demostrando que as sementes neste ponto de maturação fisiológica, encontra-se bem formadas e que a secagem não provocou danos em suas membranas celulares.

https://doi.org/10.5935/1806-6690.20230026

Lesão Miocárdica e Prognóstico em Pacientes Hospitalizados com COVID-19 no Brasil: Resultados do Registro Nacional de COVID-19
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Barbosa, Hannah Cardoso

; Martins, Maria Auxiliadora Parreiras

; Jesus, Jordana Cristina de

; Meira, Karina Cardoso

; Passaglia, Luiz Guilherme

; Sacioto, Manuela Furtado

; Bezerra, Adriana Falangola Benjamin

; Schwarzbold, Alexandre Vargas

; Maurílio, Amanda de Oliveira

; Farace, Barbara Lopes

; Silva, Carla Thais Cândida Alves da

; Cimini, Christiane Corrêa Rodrigues

; Silveira, Daniel Vitorio

; Carazai, Daniela do Reis

; Ponce, Daniela

; Costa, Emanuel Victor Alves

; Manenti, Euler Roberto Fernandes

; Cenci, Evelin Paola de Almeida

; Bartolazzi, Frederico

; Madeira, Glícia Cristina de Castro

; Nascimento, Guilherme Fagundes

; Velloso, Isabela Vasconcellos Pires

; Batista, Joanna d’Arc Lyra

; Morais, Júlia Drumond Parreiras de

; Carvalho, Juliana da Silva Nogueira

; Ruschel, Karen Brasil

; Martins, Karina Paula Medeiros Prado

; Zandoná, Liege Barella

; Menezes, Luanna Silva Monteiro

; Kopittke, Luciane

; Castro, Luís César de

; Nasi, Luiz Antônio

; Floriani, Maiara Anschau

; Souza, Maíra Dias

; Carneiro, Marcelo

; Bicalho, Maria Aparecida Camargos

; Lima, Maria Clara Pontello Barbosa

; Godoy, Mariana Frizzo de

; Guimarães-Júnior, Milton Henriques

; Mendes, Paulo Mascarenhas

; Delfino-Pereira, Polianna

; Ribeiro, Raquel Jaqueline Eder

; Finger, Renan Goulart

; Menezes, Rochele Mosmann

; Francisco, Saionara Cristina

; Araújo, Silvia Ferreira

; Oliveira, Talita Fischer

; Oliveira, Thainara Conceição de

; Polanczyk, Carisi Anne

; Marcolino, Milena Soriano

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 2 elocation e20220151

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background Cardiovascular complications of COVID-19 are important aspects of the disease’s pathogenesis and prognosis. Evidence on the prognostic role of troponin and myocardial injury in Latin American hospitalized COVID-19 patients is still scarce. Objectives To evaluate myocardial injury as independent predictor of in-hospital mortality and invasive mechanical ventilation support in hospitalized patients, from the Brazilian COVID-19 Registry. Methods This cohort study is a substudy of the Brazilian COVID-19 Registry, conducted in 31 Brazilian hospitals of 17 cities, March-September 2020. Primary outcomes included in-hospital mortality and invasive mechanical ventilation support. Models for the primary outcomes were estimated by Poisson regression with robust variance, with statistical significance of p<0.05. Results Of 2,925 patients (median age of 60 years [48-71], 57.1% men), 27.3% presented myocardial injury. The proportion of patients with comorbidities was higher among patients with cardiac injury (median 2 [1-2] vs. 1 [0-2]). Patients with myocardial injury had higher median levels of brain natriuretic peptide, lactate dehydrogenase, creatine phosphokinase, N-terminal pro-brain natriuretic peptide, and C-reactive protein than patients without myocardial injury. As independent predictors, C-reactive protein and platelet counts were related to the risk of death, and neutrophils and platelet counts were related to the risk of invasive mechanical ventilation support. Patients with high troponin levels presented a higher risk of death (RR 2.03, 95% CI 1.60-2.58) and invasive mechanical ventilation support (RR 1.87, 95% CI 1.57-2.23), when compared to those with normal troponin levels. Conclusion Cardiac injury was an independent predictor of in-hospital mortality and the need for invasive mechanical ventilation support in hospitalized COVID-19 patients.

Resumo Fundamento As complicações cardiovasculares da COVID-19 são aspectos importantes da patogênese e do prognóstico da doença. Evidências do papel prognóstico da troponina e da lesão miocárdica em pacientes hospitalizados com COVID-19 na América Latina são ainda escassos. Objetivos Avaliar a lesão miocárdica como preditor independente de mortalidade hospitalar e suporte ventilatório mecânico em pacientes hospitalizados, do registro brasileiro de COVID-19. Métodos Este estudo coorte é um subestudo do registro brasileiro de COVID-19, conduzido em 31 hospitais brasileiros de 17 cidades, de março a setembro de 2020. Os desfechos primários incluíram mortalidade hospitalar e suporte ventilatório mecânico invasivo. Os modelos para os desfechos primários foram estimados por regressão de Poisson com variância robusta, com significância estatística de p<0,05. Resultados Dos 2925 pacientes [idade mediana de 60 anos (48-71), 57,1%], 27,3% apresentaram lesão miocárdica. A proporção de pacientes com comorbidades foi maior nos pacientes com lesão miocárdica [mediana 2 (1-2) vs. 1 (0-20)]. Os pacientes com lesão miocárdica apresentaram maiores valores medianos de peptídeo natriurético cerebral, lactato desidrogenase, creatina fosfoquinase, N-terminal do pró-peptídeo natriurético tipo B e proteína C reativa em comparação a pacientes sem lesão miocárdica. Como fatores independentes, proteína C reativa e contagem de plaquetas foram relacionados com o risco de morte, e neutrófilos e contagem de plaquetas foram relacionados ao risco de suporte ventilatório mecânico invasivo. Os pacientes com níveis elevados de troponina apresentaram um maior risco de morte (RR 2,03, IC95% 1,60-2,58) e suporte ventilatório mecânico (RR 1,87;IC95% 1,57-2,23), em comparação àqueles com níveis de troponina normais. Conclusão Lesão cardíaca foi um preditor independente de mortalidade hospitalar e necessidade de suporte ventilatório mecânico em pacientes hospitalizados com COVID-19.

https://doi.org/10.36660/abc.20220151

Low-cost system for multispectral image acquisition and its applicability to analysis of the physiological potential of soybean seeds
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Soares, Júlia Martins

; Medeiros, André Dantas de

; Pinheiro, Daniel Teixeira

; Rosas, Jorge Tadeu Fim

; Silva, Laércio Junio da

; Machado, Daniel Lucas Magalhães

; Dias, Denise Cunha Fernandes dos Santos

Acta Scientiarum. Agronomy

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2023, Volume 45 elocation e57060

Resumo: En | Texto: En | PDF: En

ABSTRACT. The use of multispectral images has great potential to assess seed quality and represents a significant technological advance in the search for fast and non-destructive analysis techniques. However, the devices currently available are expensive. Thus, this study aimed to propose a low-cost method for acquisition and processing of multispectral images of soybean seeds and to evaluate their potential for rapid determination of seed physiological potential. The study was conducted in three steps: implementation of the multispectral image acquisition system, development of an algorithm for automatic image processing, and evaluation of the relationship between the data obtained through image analysis and the results of standard tests used to evaluate seed physiological potential. A total of 43 variables were assessed, eight related to seed physiological potential (germination and vigor) and 35 obtained from the analysis of the multispectral images. Of the variables obtained from multispectral images, 21 were related to pixel values in the images in the different bands evaluated (green, red, and infrared) and 14 associated with seed morphometric characteristics. The proposed system is efficient in obtaining multispectral images and the algorithm developed was efficient to extract morphometric characteristics and pixel information from the images. The parameters obtained from the NIR spectrum region showed a good relationship with the physiological potential of soybean seeds.

https://doi.org/10.4025/actasciagron.v45i1.57060

Oxidative stress, protein metabolism, and physiological potential of soybean seeds under weathering deterioration in the pre-harvest phase
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Pinheiro, Daniel Teixeira

; Dias, Denise Cunha Fernandes dos Santos

; Silva, Laércio Junio da

; Martins, Maycon Silva

; Finger, Fernando Luiz

Acta Scientiarum. Agronomy

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2023, Volume 45 elocation e56910

Resumo: En | Texto: En | PDF: En

ABSTRACT. Weathering deterioration affects seed quality, especially in areas with excessive rainfall. This study aimed to evaluate the oxidative stress, physiological quality, and protein metabolism of seeds of different soybean cultivars under weathering deterioration at the pre-harvest phase. Six soybean cultivars (BMX Apolo, DM 6563, NS 5959, NA 5909, BMX Potência, and TMG 1175) were subjected to simulated rainfall at the R8 stage. Each level was divided into two applications at 72-h intervals: 60 mm (30 + 30), 120 mm (60 + 60), and 180 mm (90 + 90). Then, the seeds were harvested and evaluated for physiological potential, antioxidative enzymes, hydrogen peroxide, malondialdehyde, proteins, and protease activity. The simulated rainfall allowed the variation in seed moisture, promoting a significant reduction in germination and seed vigor, especially at 120 and 180 mm levels. There were also reductions in antioxidative enzyme activity with weathering deterioration (mainly for catalase, ascorbate peroxidase, and peroxidase), accumulation of hydrogen peroxide and malondialdehyde, and reductions in protein content and protease activity. The proposed rainfall system is efficient in inducing weathering deterioration during the pre-harvest phase and its deleterious effects. Weathering deterioration in soybean seeds in the pre-harvest stage is directly influenced by genotype.

https://doi.org/10.4025/actasciagron.v45i1.56910

Rapid antigen test as a tool for the identification of SARS-CoV-2 infection and its potential as a self-testing device
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Filgueiras, Priscilla Soares

; Corsini, Camila Amormino

; Almeida, Nathalie Bonatti Franco

; Pedrosa, Maria Luysa Camargos

; Miranda, Daniel Alvim Pena de

; Gomes, Sarah Vieira Contin

; Assis, Jéssica Vieira de

; Silva, Raphael Antônio

; Medeiros, Maria Izabella Vieira de Assis Rocha Carvalho de

; Lourenço, Adelina Junia

; Bicalho, Cecilia Maria Florencio

; Vilela, Raquel Virginia Rocha

; Jeremias, Wander de Jesus

; Fernandes, Gabriel da Rocha

; Queiroz, Rafaella Fortini Grenfell e

Revista da Sociedade Brasileira de Medicina Tropical

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2023, Volume 56 elocation e0167-2022

Resumo: En | Texto: En | PDF: En

ABSTRACT Background: SARS-CoV-2 virus originated in Wuhan (China) in December (2019) and quickly spread worldwide. Antigen tests are rapid diagnostic tests (RDT) that produce results in 15-30 min and are an important tool for the scale-up of COVID-19 testing. COVID-19 diagnostic tests are authorized for self-testing at home in some countries, including Brazil. Widespread COVID-19 diagnostic testing is required to guide public health policies and control the speed of transmission and economic recovery. Methods: Patients with suspected COVID-19 were recruited at the Hospital da Baleia (Belo Horizonte, Brazil). The SARS-CoV-2 antigen-detecting rapid diagnostic tests were evaluated from June 2020 to June 2021 using saliva, nasal, and nasopharyngeal swab samples from 609 patients. Patient samples were simultaneously tested using a molecular assay (RT-qPCR). Sensitivity, specificity, accuracy, and positive and negative predictive values were determined using the statistical program, MedCalc, and GraphPad Prism 8.0. Results: The antigen-detecting rapid diagnostic tests displayed 98% specificity, 60% sensitivity, 96% positive predictive value, and moderate concordance with RT-qPCR. Substantial agreement was found between the two methods for patients tested < 7 days of symptom onset. Conclusions: Our findings support the use of Ag-RDT as a valuable and safe diagnostic method. Ag-RDT was also demonstrated to be an important triage tool for suspected COVID-19 patients in emergencies. Overall, Ag-RDT is an effective strategy for reducing the spread of SARS-CoV-2 and contributing to COVID-19 control.

https://doi.org/10.1590/0037-8682-0167-2022

10.

NARRATIVAS DE HOMENS TRANS: UMA ANÁLISE DISCURSIVA NO FACEBOOK
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Brito, Jean Fernandes

; Martínez-Ávila, Daniel

; Silva, Rafaela Carolina da

Encontros Bibli

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2023, Volume 28 elocation e84517

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: The study analyses the use of a social network as a tool for activism of a group situated in the margins of the current political scenario of Brazil. It aims to reveal the narratives of trans men on Facebook as constructed within the group “Homens trans e simpatizantes” (Trans men and allies). Methods: It uses the Discourse Analysis, in order to develop qualitative research. Results: It was identified that the narratives involved questions related to family, health, and prejudice. The context of the trans men’s narratives remained the same in relation to the previous studies, when not, it worsened in terms of the positiveness, which directly influences in the way these subjects are understood and act in society. Conclusions: It was concluded that social media is important for activism and the socialization of experiences of these subjects in information environments.

RESUMO Objetivo: A presente pesquisa analisa o uso de uma mídia social como ferramenta de ativismo para um grupo politicamente situado na margem do cenário político atual do Brasil. O trabalho objetiva revelar como as narrativas de homens trans vêm sendo construídas em postagens do grupo do Facebook “Homens trans e simpatizantes”. Método: Utiliza o método Análise do Discurso, com abordagem qualitativa. Resultado: Identificou-se que as narrativas perpassaram por questões de família, saúde e preconceito. O contexto das narrativas de homens trans continuou o mesmo em relação a estudos anteriores, quando não, piorou em questão de positividade das mensagens, o que influi, diretamente, no modo como esses sujeitos são compreendidos e atuam em sociedade. Conclusões: Conclui-se ressaltando a importância do grupo analisado para o caso dos homens trans e o potencial das mídias sociais no processo de ativismo, como forma de sociabilidade em ambientes informacionais.

https://doi.org/10.5007/1518-2924.2023.e84517

11.

Potential of a native araticum species as the rootstock of atemoya cultivars propagated by grafting methods
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Silva, Edvan Costa da

; Villa, Fabíola

; Silva, Daniel Fernandes da

; Possenti, Jean Carlo

; Silva, Adriana de Castro Correia da

; Masiero, Michel Anderson

Acta Scientiarum. Agronomy

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2023, Volume 45 elocation e60964

Resumo: En | Texto: En | PDF: En

ABSTRACT. Technical and secure information on the production of quality atemoya seedlings (Annona squamosa L. x Annona cherimola Mill.) are of paramount importance due to the growing interest in this crop. This study aimed to assess the formation of atemoya seedlings propagated by grafting methods and cultivars using araticum (Annona sylvatica) rootstocks. The experiment was carried out in a screened environment at the Western Paraná State University (Unioeste), Campus of Marechal Cândido Rondon, Paraná State, Brazil. Atemoya grafts (Thompson, African Pride, and Gefner) were collected from plants from the CATI seedling nursery, located in São Bento do Sapucaí, São Paulo State, Brazil. Grafting was performed using 1-year-old araticum rootstocks with 8-10 mm in diameter at a height of 15 cm from the ground and grafts measuring, on average, 8.5 cm in length, 10 mm in diameter, and three buds. The experimental design consisted of randomized blocks in a 3 × 3 factorial arrangement (three grafting methods: cleft graft, whip and tongue graft, and chip budding × three cultivars: African Pride, Thompson, and Gefner), with four replications and 10 seedlings per replication, totaling 40 seedlings per treatment. The percentage of graft success (%), percentage of sprouting (%), number of sprouts, number of leaves, and longest sprout length (cm) were assessed at 60 days after grafting. The cleft graft method is efficient for producing atemoya seedlings. The cultivar African Pride can be grafted onto rootstocks by the cleft graft and whip and tongue graft methods, as they promote a higher percentage of graft success. The Annona sylvatica rootstock has potential for the production of atemoya seedlings, but chip budding is not viable.

https://doi.org/10.4025/actasciagron.v45i1.60964

12.

Effects of auriculotherapy on anxiety and biomarkers in Primary Health Care: a clinical trial
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Silva, Carina da

; Siqueira, Letícia

; Drago, Lívia Crespo

; Lins, Elisa Mitkus Flores

; Martins, Daniel Fernandes

; Bobinski, Franciane

Revista Brasileira de Enfermagem

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2023, Volume 76 Nº 6 elocation e20220728

Resumo: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: to assess the effects of auriculotherapy on anxiety and brain-derived neurotrophic factor (BDNF), neuron-specific enolase (NSE) and S100 calcium-binding protein B (S100B) serum levels in adults assisted in Primary Health Care. Methods: a pre-experimental pilot clinical trial. Information was obtained from 19 patients using the State-Trait Anxiety Inventory (STAI) and analysis of BDNF, NSE and S100B serum levels. Results: the pre-intervention anxiety score in the IDATE-Trait was 52.11±6.691 (CV 12.84%) and the assessment after auriculotherapy was significantly lower (43.72±8.141; CV 18.62%; P=0.0007). S100B levels were significantly reduced after auriculotherapy (from 64.03±72.18 to 54.03±68.53 pg/mL; CV 126.8%; P=0.0023). Conclusion: auriculotherapy effectively reduced anxiety levels. It proved to be safe and easy to apply, allowing nurses to perform this technique autonomously. A reduction of S100B was also evidenced, demonstrating possible prevention of neuronal damage.

RESUMEN Objetivo: evaluar los efectos de la auriculoterapia sobre la ansiedad y los niveles séricos de factor neurotrófico derivado de cerebro (BDNF), enolasa-específica de neurona (NSE) y proteína fijadora de calcio S100B (S100B) en adultos atendidos en Atención Primaria de Salud. Métodos: ensayo clínico piloto preexperimental. Se obtuvo información de 19 pacientes mediante el Inventario de Ansiedad Estado-Rasgo (STAI) y análisis de niveles séricos de BDNF, NSE y S100B. Resultados: la puntuación de ansiedad preintervención en el IDATE-Rasgo fue de 52,11±6,691 (CV 12,84%) y la valoración tras la auriculoterapia fue significativamente menor (43,72±8,141; CV 18,62%; P=0,0007). Los niveles de S100B se redujeron significativamente después de la auriculoterapia (de 64,03±72,18 a 54,03±68,53 pg/mL; CV 126,8%; P=0,0023). Conclusión: la auriculoterapia redujo efectivamente los niveles de ansiedad. Demostró ser seguro y fácil de aplicar, lo que permitió a las enfermeras realizar esta técnica de forma autónoma. También se evidenció una reducción de S100B, lo que demuestra una posible prevención del daño neuronal.

RESUMO Objetivo: avaliar os efeitos da auriculoterapia sobre a ansiedade e os níveis séricos do fator neurotrófico derivado do cérebro (BDNF), enolase-específica de neurônios (NSE) e proteína ligadora de cálcio S100B (S100B) em adultos atendidos na Atenção Primária à Saúde. Métodos: ensaio clínico piloto pré-experimental. As informações foram obtidas de 19 pacientes por meio do Inventário de Ansiedade Traço-Estado (IDATE) e da análise dos níveis séricos de BDNF, NSE e S100B. Resultados: o escore de ansiedade pré-intervenção no IDATE-Traço foi de 52,11±6,691 (CV 12,84%) e a avaliação após a auriculoterapia foi significativamente menor (43,72±8,141; CV 18,62%; P=0,0007). Os níveis de S100B foram significativamente reduzidos após auriculoterapia (de 64,03±72,18 para 54,03±68,53 pg/mL; CV 126,8%; P=0,0023). Conclusão: a auriculoterapia reduziu efetivamente os níveis de ansiedade. Mostrou-se segura e de fácil aplicação, possibilitando ao enfermeiro realizar esta técnica com autonomia. Uma redução de S100B também foi evidenciada, demonstrando possível prevenção de danos neuronais.

https://doi.org/10.1590/0034-7167-2022-0728pt

13.

Multidisciplinary Scientific Cruises for Environmental Characterization in the Santos Basin – Methods and Sampling Design
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Moreira, Daniel L.

; Dalto, Adriana G.

; Figueiredo JR, Alberto G.

; Valerio, Aline M.

; Detoni, Amalia M. S.

; Bonecker, Ana C. T.

; Signori, Camila N.

; Namiki, Cláudia

; Sasaki, Dalton K.

; Pupo, Daniel V.

; Silva, Danilo A.

; Kutner, Deborah S.

; Duque-Castaño, Diana C.

; Marcon, Eduardo H.

; Gallotta, Fabiana D. C.

; Paula, Fabiana S.

; Gallucci, Fabiane

; Roque, Gabriela C. F.

; Campos, Giulia S.

; Fonseca, Gustavo

; Mattos, Gustavo

; Lavrado, Helena P.

; Silveira, Ilson C. A. da

; Costa, Jessica O.

; Santos Filho, João R. dos

; Carneiro, Juliane C.

; Moreira, Julio C.F.

; Rozo, Laura

; Araujo, Leandro F.M.

; Lazzari, Letícia

; Silva, Letícia O. da

; Michelazzo, Luan S.

; Fernandes, Luciano F.

; Dottori, Marcelo

; Araújo Jr., Marcus A. G. de

; Chuqui, Mateus G.

; Ceccopieri, Milena

; Borges-Silva, Milton

; Kampel, Milton

; Bergo, Natascha M.

; Silva, Paulo V. M.

; Tura, Pedro M.

; Moura, Rafael B. de

; Romano, Renato G.

; Martins, Renato P.

; Carreira, Renato S.

; Toledo, Rodrigo G.A.

; Bonecker, Sérgio L.C.

; Disaró, Sibelle T.

; Rodrigues, Silvana V.

; Corbisier, Thais N.

; Vicente, Thaisa M.

; Paiva, Vitor G. de

; Pellizari, Vivian H.

; Belo, Wellington C.

; Brandini, Frederico P.

; Sousa, Silvia H.M

Ocean and Coastal Research

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2023, Volume 71 Supl. 3 elocation e23022

Resumo: En | Texto: En | PDF: En

Abstract The Santos Basin (SB) is the main petroliferous basin in the Brazilian continental margin and one of the most studied marine areas in Brazil. However, historical data suggest that new efforts should be carried out to acquire quantitative biological data, especially in the deep sea, to establish the baseline of essential ocean variables in different ecosystems for future monitoring programs. The Brazilian energy company Petrobras planned and executed 24 oceanographic cruises over a period of 2 years to assess the benthic (SANSED cruise) and pelagic (SANAGU cruise) systems of the SB (356 days at sea in 2019 and 2021/2022). These efforts were part of the Santos Project, which comprised a comprehensive environmental study aimed at investigating benthic and pelagic variables to characterize ecology, biogeochemistry, thermohaline properties of water masses, and ocean circulation patterns, geomorphology, and sedimentology, as well as organic and inorganic chemistry. Here we present the detailed sampling designs and the field methods employed on board, during the SB scientific cruises. All sampling protocols were based on standardized approaches. For the benthos analyses, triplicate sediment samples were performed using a GOMEX-type box corer (0.25 m²) or a large modified Van Veen grab (0.75 m²) at 100 stations ranging from 25 to 2400 m depth. At each station, 25 geochemical and physico-chemical parameters were analyzed in addition to micro-, meio-, and macrofauna and living foraminifera samples. For the pelagic system, 60 stations were selected to investigate the plankton community, ranging in size from pico- to macroplankton, through vertical, horizontal, and oblique net hauls (20, 200, and 500 μm mesh size), as well as 25 biogeochemical parameters collected with an aid of a CTD-rosette sampler. Part of this scientific information also serves the Regional Environmental Characterization Project (PCR-BS) in support of Petrobras’ Santos Basin drilling licensing process led by the Brazilian Environmental Agency – IBAMA. This project contributes to the sustainable development of the SB, in line with the guidelines of the United Nations Decade of Ocean Science for Sustainable Development.

https://doi.org/10.1590/2675-2824071.22072dlm

14.

The SISBIOTA-Diptera Brazilian Network: A long-term survey of Diptera from unexplored Brazilian Western Arc of Amazon, Cerrado, and Pantanal
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Lamas, Carlos José Einicker

; Fachin, Diego Aguilar

; Falaschi, Rafaela Lopes

; Alcantara, Daniel Máximo Correa de

; Ale-Rocha, Rosaly

; Amorim, Dalton de Souza

; Araújo, Maíra Xavier

; Ascendino, Sharlene

; Baldassio, Letícia

; Bellodi, Carolina Ferraz

; Bravo, Freddy

; Calhau, Julia

; Capellari, Renato Soares

; Carmo-Neto, Antonio Marcelino do

; Cegolin, Bianca Melo

; Couri, Márcia Souto

; Carvalho, Claudio José Barros de

; Dios, Rodrigo de Vilhena Perez

; Falcon, Aida Vanessa Gomez

; Fusari, Livia Maria

; Garcia, Carolina de Almeida

; Gil-Azevedo, Leonardo Henrique

; Gomes, Marina Morim

; Graciolli, Gustavo

; Gudin, Filipe Macedo

; Henriques, Augusto Loureiro

; Krolow, Tiago Kütter

; Mendes, Luanna Layla

; Limeira-de-Oliveira, Francisco

; Maia, Valéria Cid

; Marinoni, Luciane

; Mello, Ramon Luciano

; Mello-Patiu, Cátia Antunes de

; Morales, Mírian Nunes

; Oliveira, Sarah Siqueira

; Patiu, Claudemir

; Proença, Barbara

; Pujol-Luz, Cristiane Vieira de Assis

; Pujol-Luz, José Roberto

; Rafael, José Albertino

; Riccardi, Paula Raile

; Rodrigues, João Paulo Vinicios

; Roque, Fabio de Oliveira

; Sallum, Maria Anice Mureb

; Santis, Marcelo Domingos de

; Santos, Charles Morphy Dias dos

; Santos, Josenilson Rodrigues dos

; Savaris, Marcoandre

; Shimabukuro, Paloma Helena Fernandes

; Silva, Vera Cristina

; Schelesky-Prado, Daniel de Castro

; Silva-Neto, Alberto Moreira da

; Camargo, Alexssandro

; Sousa, Viviane Rodrigues de

; Urso-Guimarães, Maria Virginia

; Wiedenbrug, Sofia

; Yamaguchi, Carolina

; Nihei, Silvio Shigueo

Revista Brasileira de Entomologia

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2023, Volume 67 Nº 4 elocation e20230051

Resumo: En | Texto: En | PDF: En

ABSTRACT The SISBIOTA-BRASIL was a three-year multimillion-dollar research program of the Brazilian government to document plants and animals in endangered/understudied areas and biomes in Brazil. Distributional patterns and the historical events that generated them are extensively unknown regarding Brazilian fauna and flora. This deficiency hinders the development of conservation policies and the understanding of evolutionary processes. Conservation decisions depend on precise knowledge of the taxonomy and geographic distribution of species. Given such a premise, we proposed to research the diversity of Diptera of the Brazilian western arc of Amazon, Cerrado, and Pantanal in the states of Mato Grosso, Mato Grosso do Sul, and Rondônia. Three important biomes of the South American continent characterize these Brazilian states: Amazon forest, Cerrado (Brazilian Savannah), and Pantanal. Besides their ecological relevance, these biomes historically lack intensive entomological surveys. Therefore, they are much underrepresented in the Brazilian natural history collections and in the scientific literature, which is further aggravated by the fact that these areas are being exponentially and rapidly converted to commercial lands. Our project involved over 90 collaborators from 24 different Brazilian institutions and one from Colombia among researchers, postdocs, graduate and undergraduate students, and technicians. We processed and analyzed nearly 300,000 specimens from ~60 families of Diptera collected with a large variety of methods in the sampled areas. Here, we provide a detailed overview of the genera and species diversity of 41 families treated. Our results point to a total of 2,130 species and 514 genera compiled and identified for the three states altogether, with an increase of 41% and 29% in the numbers of species and genera known for the three states combined, respectively. Overall, the 10 most species-rich families were Tachinidae, Cecidomyiidae, Tabanidae, Psychodidae, Sarcophagidae, Stratiomyidae, Bombyliidae, Syrphidae, Tephritidae, and Asilidae. The 10 most diverse in the number of genera were Tachinidae, Stratiomyidae, Asilidae, Mycetophilidae, Syrphidae, Tabanidae, Muscidae, Dolichopodidae, Sarcophagidae, and Chloropidae. So far, 111 scientific papers were published regarding taxonomic, phylogenetic, and biogeographical aspects of the studied families, with the description of 101 new species and three new genera. We expect that additional publications will result from this investigation because several specimens are now curated and being researched by specialists.

https://doi.org/10.1590/1806-9665-rbent-2023-0051

15.

Hemodynamic responses during inspiratory muscle exercise in healthy young adults
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Moreira, Leila Dal Poggetto

; Silva, Lilian Pinto da

; Weiss, Vinícius Faria

; Sene, Rita de Cássia

; Mira, Pedro Augusto de Carvalho

; Trevizan, Patrícia Fernandes

; Laterza, Mateus Camaroti

; Martinez, Daniel Godoy

Fisioterapia e Pesquisa

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2023, Volume 30 elocation e23020323en

Resumo: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT The literature on hemodynamic responses during inspiratory muscle exercise (IME) lacks a consensus. To evaluate and compare hemodynamic responses during an IME session with and without resistive load, 15 sedentary men were subjected to two randomized IME sessions: one with 40% of maximal inspiratory pressure (IME 40%) and another without a resistive load (Sham), both of which were performed for two minutes over eight sessions with one-minute intervals. Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean blood pressure (MBP), total peripheral resistance (TPR), stroke volume (SV), cardiac output (CO), and heart rate (HR) were measured by infrared digital photoplethysmography during five basal minutes and during the IME sessions. One-way analysis of variance and the Student’s t test for paired data were used to analyze hemodynamic response and delta values between sessions. Effect size was evaluated by Cohen’s D. A 5% significance level was adopted. SBP responses (sham: ∆−1±2 vs. 40%: ∆−4±2mmHg, p=0.27), DBP (sham: ∆2±1 vs. 40%: ∆1±2mmHg, p=0.60) and MBP (sham: ∆2±1 vs. 40%: ∆0±2mmHg, p=0.28) were similar between sessions. HR increases were higher in the 40% IME session than in the sham session (sham: ∆9±2 vs. 40%: ∆3±2bpm, p=0.001). SV only decreased during the sham session but responses were similar between sessions (sham: ∆−2±2 vs. IME 40%: ∆−6±2ml, p=0.13). Both sessions did not change SBP, DBP, MBP, CO, and TPR, but we observed a greater increase in HR in the IME 40% session. Only the Sham session decreased SV.

RESUMEN No hay consenso en la literatura sobre las respuestas hemodinámicas durante el ejercicio muscular inspiratorio (EMI). El objetivo de este estudio fue evaluar y comparar las respuestas hemodinámicas durante una sesión de EMI con y sin carga resistiva. Para ello, quince hombres sedentarios recibieron dos sesiones aleatorias de EMI: el 40% de la presión inspiratoria máxima (EMI 40%) y sin carga resistiva (sham), realizadas durante dos minutos, ocho sesiones y a intervalos de un minuto. La presión arterial sistólica (PAS), la presión arterial diastólica (PAD), la presión arterial media (PAM), la resistencia periférica total (RPT), el volumen sistólico (VS), el gasto cardíaco (GC) y la frecuencia cardíaca (FC) se midieron mediante fotopletismografía infrarroja digital durante cinco minutos al inicio y durante las sesiones de EMI. Se utilizaron ANOVA unidireccional y la prueba t de Student a datos emparejados para analizar la respuesta hemodinámica y los valores delta entre las sesiones. El tamaño del efecto se evaluó por el d de Cohen. El nivel de significancia adoptado fue de 5%. Las respuestas de PAS (sham: Δ−1±2 vs. 40%: ∆−4±2mmHg, p=0,27), PAD (sham: ∆2±1 vs. 40%: ∆1±2mmHg, p=0,60) y PAM (sham: ∆2±1 vs. 40%: ∆0±2mmHg, p=0,28) fueron similares entre las sesiones. El incremento de la FC fue mayor en la sesión de EMI 40% comparada con la sesión sham (sham: Δ9±2 vs. 40%: ∆3±2bpm, p=0,001). El VS tuvo una disminución exclusiva durante la sesión sham, pero la respuesta fue similar entre las sesiones (sham: Δ−2±2 vs. EMI 40%: ∆−6±2ml, p=0,13). Ambas sesiones no tuvieron cambios en las variables PAS, PAD, PAM, DC y RPT, pero se observó un mayor incremento de la FC en la sesión EMI 40%. Solamente en la sesión sham hubo una reducción del VS.

RESUMO A literatura carece de um consenso sobre respostas hemodinâmicas durante o exercício muscular inspiratório (EMI). Este estudo buscou avaliar e comparar as respostas hemodinâmicas durante uma sessão de EMI com e sem carga resistiva. Para tanto, 15 homens sedentários foram submetidos a duas sessões randomizadas de EMI: 40% da pressão inspiratória máxima (EMI 40%) e sem carga resistiva (sham), realizadas por dois minutos em oito sessões e com intervalos de um minuto. A pressão arterial sistólica (PAS), pressão arterial diastólica (PAD), pressão arterial média (PAM), resistência periférica total (RPT), volume sistólico (VS), débito cardíaco (DC) e frequência cardíaca (FC) foram medidos por fotopletismografia infravermelha digital por cinco minutos basais e durante as sessões de EMI. Anova de uma via e o teste t de Student para dados pareados foram usados para analisar a resposta hemodinâmica e os valores delta entre as sessões. O tamanho do efeito foi avaliado pelo d de Cohen. Adotou-se nível de significância de 5%. As respostas de PAS (sham: ∆−1±2 vs. 40%: ∆−4±2mmHg, p=0,27), PAD (sham: ∆2±1 vs. 40%: ∆1±2mmHg, p=0,60) e PAM (sham: ∆2±1 vs. 40%: ∆0±2mmHg, p=0,28) foram semelhantes entre as sessões. Os aumentos da FC foram maiores na sessão de EMI 40% do que nas sessões sham (sham: ∆9±2 vs. 40%: ∆3±2bpm, p=0,001). O VS diminuiu exclusivamente durante a sessão sham mas a resposta foi semelhante entre as sessões (sham: ∆−2±2 vs. EMI 40%: ∆−6±2ml, p=0,13). Ambas as sessões não causaram alteração nas variáveis PAS, PAD, PAM, DC e RPT, mas notamos um aumento maior da FC na sessão EMI 40%. Apenas a sessão sham reduziu o VS.

https://doi.org/10.1590/1809-2950/e23020323en

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