Abstract The aim of this study was to identify factors associated with early identification of disabilities and developmental follow-up of children in primary health care (PHC) services under the Care Network for People with Disabilities (RCPCD). We conducted a cross-sectional study using data from a multicenter study undertaken in eight states. The data were collected using a structured questionnaire answered by PHC professionals with degree-level qualifications selected using random sampling and stratified by state and municipality. Poisson regression with robust variance was performed for the two outcomes. Of the 1,488 workers in the final sample, 63.6% performed early identification of disabilities and 49% provided developmental follow-up. Family health teams performed early identification of disabilities and follow-up more than traditional model teams, and expanded family health teams provided developmental follow-up more than both these teams. The factors that showed the strongest association with identification and developmental follow-up were profession, working in a family health team and knowledge of the RCPCD. followup follow up (PHC RCPCD . (RCPCD) crosssectional cross sectional states degreelevel degree level municipality outcomes 1488 1 488 1,48 sample 636 63 6 63.6 49 followup. up. profession (RCPCD 148 48 1,4 63. 4 14 1,

Resumo Objetivou-se identificar fatores associados à atenção à saúde infantil na atenção primária à saúde (APS), na perspectiva das ações preconizadas pela Rede de Cuidados à Pessoa Deficiência (RCPCD), quanto à identificação precoce de deficiências e acompanhamento do desenvolvimento infantil. Trata-se de estudo transversal, multicêntrico, realizado em oito estados brasileiros. A coleta de dados envolveu a aplicação de questionário estruturado com profissionais de saúde de nível superior atuantes na APS, com amostra aleatória estratificada por estado e município. Foram realizadas regressões de Poisson com variância robusta para duas variáveis resposta. Entre os 1.488 trabalhadores que compuseram a amostra final, 63,6% realizam ações de identificação precoce de deficiências e 49% efetuam acompanhamento do desenvolvimento infantil. As equipes de Saúde da Família (eSF) identificam e acompanham mais do que as equipes do modelo tradicional, e os Núcleos Ampliados de Saúde da Família e Atenção Básica realizam mais ações de acompanhamento do que essas equipes. Conclui-se que os fatores mais associados com a identificação e o acompanhamento de crianças com deficiências na APS foram aqueles relativos à formação profissional, à eSF e ao conhecimento de normativas relativas à RCPCD. Objetivouse Objetivou se , (APS) RCPCD, RCPCD (RCPCD) Tratase Trata transversal multicêntrico brasileiros município resposta 1488 1 488 1.48 final 636 63 6 63,6 49 (eSF tradicional Concluise Conclui profissional (APS (RCPCD 148 48 1.4 63, 4 14 1.

Introduction: Cholesteatoma, although relatively rare, presents significant challenges in clinical practice. Despite decades of research, its cause remains complex and elusive. Objective: This review aimed to summarize key findings on the epidemiology, pathophysiology, diagnosis and surgical management of cholesteatoma, highlighting current knowledge gaps and emerging trends in the field. Method: Narrative review carried out in the PubMed and Scielo databases. The search used the following keywords: “cholesteatoma, petros apex, minimally invasive surgical procedures”. The inclusion criteria comprised review articles, pre-clinical and clinical research published, written in Portuguese and English and available in complete text. The selected articles were analyzed based on the technologies covered, future perspectives, challenges, diseases mentioned and the central idea of the article. Result: 36 articles were included. Conclusion: Cholesteatoma is important in medical practice, despite being relatively uncommon in the general population. However, there are still doubts about its cause, but its diagnosis has been expanded through imaging tests in an attempt to reduce “second-look” operations and avoid them. Another field with constant growth is surgical treatment, since the complex anatomy requires that new techniques be managed to reduce injuries to important and vital structures in the region.

Introdução: O colesteatoma, embora relativamente raro, apresenta desafios significativos na prática clínica. Apesar de décadas de pesquisa, sua causa permanece complexa e evasiva. Objetivo: Esta revisão pretendeu resumir as principais descobertas sobre a epidemiologia, fisiopatologia, diagnóstico e manejo cirúrgico do colesteatoma, destacando lacunas de conhecimento atuais e tendências emergentes no campo. Método: Revisão narrativa realizada nas bases PubMed e Scielo. A busca utilizou as seguintes palavras-chave: “colesteatoma, ápice petros, procedimentos cirúrgicos minimamente invasivos” e suas versões ao inglês Os critérios de inclusão compreenderam artigos de revisão, pesquisas pré-clínicas e clínicas publicados, escritos em português e inglês e disponíveis em texto completo. Os artigos selecionados foram analisados com base nas tecnologias abordadas, perspectivas futuras, desafios, doenças referidas e ideia central do artigo. Resultado: Foram incluídos 36 artigos. Conclusão: O colesteatoma tem importância na prática médica, apesar de ser relativamente pouco frequente na população em geral. No entanto, ainda existem dúvidas sobre sua causa, mas seu diagnóstico tem sido ampliado atavés de exames de imagem na tentativa de redução das operações de “segundo-olhar”, evitando-as. Outro campo com crescimento constante é o tratamento cirúrgico, uma vez que a anatomia complexa exige que novas técnicas sejam manejadas para diminuir lesões de estruturas importantes e vitais da região.

Abstract Introduction: Suffering is complex and individual. It is essential to assess it for healthcare. The objective was to map the literature about the patients suffering in Palliative Care. Method: It was performed a scoping review, based on the Joanna Briggs Institute protocol. The research was carried out in three databases: CINAHL, Pubmed and Web of Science (studies published between January 1, 2017 and October 12, 2022). Results: Six articles were analyzed, published in Portugal, Italy and Canada, into inpatient and home settings. Interpretation of results and conclusion: Were identified 3 dimensions of suffering, somatic suffering, non-somatic suffering and experiential responses to disease situations. It is concluded that the perception of suffering is unique for each patient, with non-somatic suffering predominance, and its intensity is related to the way the patient reads his experiences.

Resumo Introdução: O sofrimento é complexo e individual, sendo a sua avaliação fundamental para os cuidados. Estabeleceu-se como objetivo mapear a literatura acerca do sofrimento do doente em Cuidados Paliativos. Método: Realizou-se uma scoping review de acordo com o protocolo do Joanna Briggs Institute. A pesquisa foi realizada em três bases de dados: CINAHL, Pubmed e Web of Science (estudos publicados entre 01 de janeiro de 2017 e 12 de outubro de 2022). Resultados: Foram analisados 6 artigos, originários de Portugal, Itália e Canadá, em contexto de internamento e de domicílio. Interpretação dos resultados e conclusão: Como dimensões do sofrimento identificaram-se o sofrimento somático, o sofrimento não somático e as respostas vivenciais às situações de doença. Conclui-se que a perceção do sofrimento é única, com predomínio do sofrimento não somático, encontrando-se a sua intensidade relacionada com a forma como o doente vivencia as suas experiências.

Resumo A síndrome hemolítica-urémica mediada por complemento (SHUc) é uma síndrome rara, que atinge predominantemente crianças e jovens adultos e surge por defeitos na regulação da cascata do complemento. Apresentamos o caso de uma doente que se apresenta com quadro de náuseas, vómitos, dor abdominal difusa, dejeções e colúria com três dias de evolução. Analiticamente com sinais de anemia hemolítica microangiopática, trombocitopenia e lesão renal, foi internada para estudo. Inicialmente sob terapêutica com permuta plasmática e corticoterapia, sem melhoria clínica ou analítica. Após estudo exaustivo, e excluídos diagnósticos mais prováveis, assumido diagnóstico de SHUc tendo iniciado terapêutica dirigida com eculizumab com recuperação sustentada.

Abstract Complement-mediated hemolytic uremic syndrome (cHUS) is a rare syndrome that predominantly affects children and young adults and arises from defects in the regulation of the complement cascade. We present the case of a patient who presented with a clinical picture of nausea, vomiting, diffuse abdominal pain, diarrhea, and dark urine with three days of evolution. Analytically, there were signs of microangiopathic hemolytic anemia, thrombocytopenia, and renal injury, and the patient was admitted for study. Initially treated with plasmapheresis and corticosteroids, without clinical or analytical improvement. After an exhaustive study, and with more likely diagnoses excluded, cHUS was assumed and treatment with eculizumab was initiated with sustained recovery.

Resumo Introdução: A promoção da literacia em saúde é crucial para que os utentes possam tomar decisões adequadas em matéria de saúde, promovendo uma utilização mais eficiente dos recursos e contribuindo para a sua sustentabilidade. Objetivos: Promover a literacia em saúde entre os utentes de seis Unidades de Saúde Familiar (USF) no Norte de Portugal acerca da organização e estrutura dos cuidados de saúde primários (CSP) e a especialidade médica de medicina geral e familiar (MGF). Métodos: Estudo observacional, prospetivo, do tipo coorte. Foram aplicados questionários em formato Google® Docs a utentes com 18 ou mais anos, que preenchiam os critérios de inclusão, antes e após um mês da intervenção. A intervenção consistiu na visualização de um vídeo formativo preparado pelos autores acerca da organização e os recursos dos CSP e a especialidade de MGF. Resultados: O questionário pré-intervenção foi preenchido por 274 utentes, 165 visualizaram o vídeo educativo e 113 preencheram o questionário pós-intervenção. Apenas 88 utilizadores participaram em todas as fases do projeto e foram considerados elegíveis para a interpretação dos resultados. Após a intervenção verificou-se uma melhoria estatisticamente significativa na classificação geral obtida no questionário, com um aumento na percentagem de respostas corretas de 66,7% para 72,7% (p=0,004; IC 95%). Verificou-se também um aumento na taxa de respostas corretas em quase todas as questões aplicadas. Discussão e Conclusão: A literacia em saúde ajuda os cidadãos a tomar melhores decisões em relação à sua saúde, com um consequente impacto económico e social. Este projeto culminou na criação de uma ferramenta multimédia capaz de melhorar os conhecimentos dos utentes portugueses acerca dos CSP, passível de ser replicada noutros locais do País.

Abstract Introduction: Promoting health literacy is essential for patients to make informed healthcare decisions, ensuring more efficient resource utilization and contributing to long-term sustainability. Objectives: Enhance health literacy among patients in six health units in Northern Portugal, focusing on the organization and structure of primary healthcare services (PHC) and the medical specialty of general practice/family medicine (GPFM). Methods: An observational, prospective cohort study was conducted. Questionnaires in Google® Docs format were administered to patients aged 18 and older who met the inclusion criteria, both before and one month after the intervention. The intervention involved watching an educational video created by the authors about PHC's organization, resources, and GPFM specialty. Results: The pre-intervention questionnaire received responses from 274 patients, with 165 of them viewing the educational video and 113 completing the post-intervention questionnaire. Eighty-eight participants engaged in all phases of the project and were considered eligible for result analysis. After the intervention, there was a statistically significant improvement in the overall questionnaire score, with the percentage of correct answers increasing from 66.7% to 72.7% (p=0.004; CI 95%). Correct answer rates also improved across most of the questions. Discussion and Conclusion: Health literacy empowers individuals to make informed health choices, resulting in economic and social benefits. This project led to the creation of a multimedia tool that can enhance the understanding of Portuguese patients regarding PHC, with potential for replication in other regions of the country.

Abstract Background The oral cavity is a link between of external environment with gastrointestinal tract. Studies are controversial on the presence of Periodontal Disease (PD) and its association with Gastric Adenocarcinoma (GAC). Methods The authors performed a systematic review and meta-analysis to verify the association between PD and GAC. Six electronic databases were evaluated between 1961 and 2022. Titles and abstracts were reviewed independently according to the eligibility criteria, assessing full texts of selected studies. The quality of the included research was verified using the Newcastle-Ottawa Scale for case-control and cohort studies. Statistical analyses were performed based on fixed and/or random effects models to calculate the summarized Relative Risk (RR) and its 95 % Confidence Interval (95 % CI). Results There were 639 studies, of which nine articles were included (3 case-controls and 6 cohorts). Overall, the authors identified 1,253 cases of GAC 2,501 controls in case-control studies, and 1,631 patients with GAC enrolled in cohort studies. Patients presenting PD increased the risk of developing GAC by 17 % (RR=1.17; 95 % CI 1.03‒1.32), which remained regardless of the diagnostic method for PD, i.e., clinical examination (RR = 1.19; 95 % CI 1.14‒1.24) and self-report (RR = 1.34; 95 % CI 1.06‒1.69). Moreover, Asian patients (RR=1.17; 95 % CI 1.00‒1.36) with PD had a higher risk of having GAC than American and European patients (RR = 1.18; 95 % CI 0.84‒1.66). Conclusions The presence of PD the risk of GAC suggesting that its infectious-inflammatory process of PD may be related to GAC development. Further investigations on the oral-gastric microbiota and its role in the carcinogenesis of gastric cancer should be carried out, and the screening of patients with potential risk for GAC should be considered in the clinical practice of dentists. tract (PD . (GAC) metaanalysis meta analysis 196 2022 criteria studies NewcastleOttawa Newcastle Ottawa casecontrol case control andor or RR 9 (9 CI. CI) 63 3 ( casecontrols cohorts. cohorts cohorts) Overall 1253 1 253 1,25 2501 2 501 2,50 1631 631 1,63 RR=1.17 RR117 (RR=1.17 1.03‒1.32, 103132 1.03‒1.32 , 03 32 1.03‒1.32) ie i e i.e. 1.19 119 19 1.14‒1.24 114124 14 24 selfreport self report 1.34 134 34 1.06‒1.69. 106169 1.06‒1.69 06 69 1.06‒1.69) Moreover 1.00‒1.36 100136 00 36 1.18 118 18 0.84‒1.66. 084166 0.84‒1.66 0 84 66 0.84‒1.66) infectiousinflammatory infectious inflammatory development oralgastric out dentists (GAC 202 125 25 1,2 250 50 2,5 163 1,6 RR=1.1 RR11 (RR=1.1 10313 1.03‒1.3 i.e 1.1 11 1.14‒1.2 11412 1.3 13 10616 1.06‒1.6 1.00‒1.3 10013 08416 0.84‒1.6 8 20 12 1, 5 2, 16 RR=1. RR1 (RR=1. 1031 1.03‒1. 1. 1.14‒1. 1141 1061 1.06‒1. 1.00‒1. 1001 0841 0.84‒1. RR=1 (RR=1 103 1.03‒1 1.14‒1 114 106 1.06‒1 1.00‒1 100 084 0.84‒1 RR= (RR= 10 1.03‒ 1.14‒ 1.06‒ 1.00‒ 08 0.84‒ 1.03 1.14 1.06 1.00 0.84 1.0 0.8 0.

Abstract Paper aims This study aims to identify the advances in the literature related to Sustainable Life Cycle Assessment in the agricultural and food process sectors. Originality This study pioneers an investigation into trends in applying Life Cycle Sustainability Assessment techniques within the agricultural and food processing sectors, with a comprehensive consideration of environmental, economic, and social perspectives. Research method A systematic literature review and a bibliometric analysis revealed 71 articles that applied at least one of the life cycle assessment tools. Main findings The bibliometric analysis indicated that the studied areas have two main areas that separate studies in the food process sector and the agricultural sector areas. The content analysis indicated that most studies apply the environmental assessment of the life cycle, coupling some studies with the economic and social view and mainly using an attributional approach with the scope ranging from the cradle to the grave regarding the area. Implications for theory and practice As for theory, this study includes advancing knowledge and filling a research gap, while the practical relates to more sustainable decision-making by professionals involved in the agricultural and food processing sectors. sectors perspectives 7 tools area gap decisionmaking decision making

ABSTRACT Objective: This study evaluated the accuracy and precision of digital models acquisition using a home-built, low-cost scanning system based on the structured light method. Methods: a plaster model (PM) was scanned using the experimental device (SL) and a dental desktop scanner (DS). The teeth dimensions of PM and SL models were measured in triplicate, with a caliper and digitally, respectively. The agreement of the measurements of each model was evaluated using the intraclass correlation coefficient, and the validity between the different measurement techniques was assessed using the Bland-Altman analysis. The accuracy and precision of the models were qualitatively investigated using the mesh superposition of the SL and DS models. Results: A high intraclass correlation coefficient was observed in all models (PM=0.964; SL1=0.998; SL2=0.995; SL3=0.998), and there was no statistical difference between the measurements of the SL models (p>0.05). PM and SL model measurements were found to be in good agreement, with only 3.57% of the observed differences between the same measurement being located outside 95% limits of agreement according to Bland and Altman (0.43 and -0.40 mm). In the superimpositions of SL-SL and SL-DS models, areas of discrepancy greater than 0.5 mm were observed mainly in interproximal, occlusal, and cervical sites. Conclusion: These results indicate that the home-built SL scanning system did not possess sufficient accuracy and precision for many clinical applications. However, the consistency in preserving the dental proportions suggests that the equipment can be used for planning, storage, and simple clinical purposes. Objective homebuilt, homebuilt home built, built lowcost low cost method Methods (PM (SL DS. . (DS) triplicate digitally respectively BlandAltman analysis Results PM=0.964 PM0964 0 964 (PM=0.964 SL1=0.998 SL10998 SL1 998 SL2=0.995 SL20995 SL2 995 SL3=0.998, SL30998 SL3=0.998 , SL3 SL3=0.998) p>0.05. p005 p p>0.05 05 (p>0.05) 357 3 57 3.57 95 0.43 043 43 (0.4 0.40 040 40 -0.4 mm. mm) SLSL SLDS 5 0. interproximal occlusal sites Conclusion applications However planning storage purposes (DS PM=0.96 PM096 96 (PM=0.96 SL1=0.99 SL1099 99 SL2=0.99 SL2099 SL3099 SL3=0.99 p00 p>0.0 (p>0.05 35 3.5 9 0.4 04 4 (0. -0. PM=0.9 PM09 (PM=0.9 SL1=0.9 SL109 SL2=0.9 SL209 SL309 SL3=0.9 p0 p>0. (p>0.0 3. (0 -0 PM=0. PM0 (PM=0. SL1=0. SL10 SL2=0. SL20 SL30 SL3=0. p>0 (p>0. ( - PM=0 (PM=0 SL1=0 SL2=0 SL3=0 p> (p>0 PM= (PM= SL1= SL2= SL3= (p> (p

RESUMO Objetivo: Este estudo avaliou a acurácia e a precisão da aquisição de modelos digitais utilizando um sistema de digitalização caseiro e de baixo custo baseado no método de luz estruturada. Material e Métodos: Para isso, um modelo de gesso (MG) foi digitalizado utilizando o dispositivo experimental (LE) e um scanner dental de mesa (SD). As dimensões dos dentes dos modelos MG e LE foram medidas em triplicata com um paquímetro e digitalmente, respectivamente. A concordância das medidas de cada modelo foi avaliada usando o coeficiente de correlação intraclasse, e a validade entre as diferentes técnicas de medição foi avaliada usando a análise de Bland-Altman. A acurácia e a precisão dos modelos foram investigadas qualitativamente usando a sobreposição de malhas dos modelos LE e SD. Resultados: Um alto coeficiente de correlação intraclasse foi observado em todos os modelos (MG=0,964; LE1=0,998; LE2=0,995; LE3=0,998) e não houve diferença estatística entre as medições dos modelos LE (p>0,05). As medições dos modelos MG e LE mostraram boa concordância, com apenas 3,57% das diferenças observadas entre as mesmas medições localizadas fora dos limites de concordância de 95% de acordo com a análise de Bland-Altman (0,43 e -0,40 mm). Nas sobreposições dos modelos LE-LE e LE-SD, foram observadas áreas de discrepância maiores que 0,5 mm principalmente nos sítios interproximais, oclusais e cervicais. Conclusões: Esses resultados indicam que o sistema de digitalização por luz estruturada caseiro não possui precisão e acurácia suficientes para muitas aplicações clínicas. No entanto, a consistência na preservação das proporções dentárias sugere que o equipamento pode ser usado para planejamento, armazenamento e propósitos clínicos simples. Objetivo Métodos isso (MG (LE SD . (SD) digitalmente respectivamente BlandAltman. BlandAltman Bland Altman. Altman Resultados MG=0,964 MG0964 0 964 (MG=0,964 LE1=0,998 LE10998 LE1 998 LE2=0,995 LE20995 LE2 995 LE3=0,998 LE30998 LE3 p>0,05. p005 p p>0,05 05 (p>0,05) 357 3 57 3,57 95 0,43 043 43 (0,4 0,40 040 40 -0,4 mm. mm) LELE LESD, LESD SD, LE-SD 5 0, interproximais cervicais Conclusões clínicas entanto planejamento simples (SD MG=0,96 MG096 96 (MG=0,96 LE1=0,99 LE1099 99 LE2=0,99 LE2099 LE3=0,99 LE3099 p00 p>0,0 (p>0,05 35 3,5 9 0,4 04 4 (0, -0, MG=0,9 MG09 (MG=0,9 LE1=0,9 LE109 LE2=0,9 LE209 LE3=0,9 LE309 p0 p>0, (p>0,0 3, (0 -0 MG=0, MG0 (MG=0, LE1=0, LE10 LE2=0, LE20 LE3=0, LE30 p>0 (p>0, ( - MG=0 (MG=0 LE1=0 LE2=0 LE3=0 p> (p>0 MG= (MG= LE1= LE2= LE3= (p> (p

Abstract Large segmental tracheal defects can pose a serious clinical challenge owing to the lack of suitable substitutes for reconstructive surgery. Polymeric biomaterials are widely used in medicine. However, the implantation of biomaterials triggers a series of biological events, and material biocompatibility is of paramount importance in regenerative medicine. The objective of this study was to evaluate the use of a copolyamide associated with thermoplastic elastomer (PCTPE) in 3D-printed prostheses for repairing partial tracheal defects in rabbits. Sixteen male New Zealand White rabbits were used, and partial tracheal defects were created in the ventral region from the 4th to the 8th tracheal ring. The animals were subdivided into groups (n=4) based on the time of euthanasia, scheduled at seven days (G7), 15 days (G15), 30 days (G30), and 60 days (G60). Histopathological analysis with hematoxylin and eosin staining revealed that the 3D implant of PCTPE exhibited a foreign body reaction, and inflammation persisted for up to 60 days. Histochemistry with picrosirius red revealed a tendency for a greater amount of type I collagen to accumulate in the early stages of inflammation, whereas type III collagen was predominant in later evaluation periods. These findings suggest an exacerbated and inadequate tissue-material interface reaction and perpetuation of the inflammatory process. surgery medicine However events (PCTPE 3Dprinted Dprinted printed D th ring n=4 n4 n 4 (n=4 euthanasia G7, G7 G , (G7) 1 G15, G15 (G15) 3 G30, G30 (G30) 6 G60. G60 . (G60) periods tissuematerial tissue process n= (n= (G7 G1 (G15 G3 (G30 G6 (G60 (n (G (G1 (G3 (G6

Resumo Defeitos de grandes segmentos traqueais podem apresentar sério problema clínico, devido à ausência de substitutos na cirurgia reconstrutiva. Os biomateriais poliméricos estão entre os mais utilizados na medicina. Entretanto, a implantação de um biomaterial desencadeia uma série de eventos biológicos e a biocompatibilidade do material é de extrema importância para a medicina regenerativa. Objetivou-se com este estudo avaliar o uso da copoliamida associada ao elastômero termoplástico (PCTPE) em próteses impressas em 3D para reparação de defeitos parciais da traqueia de coelhos. Foram utilizados 16 coelhos machos da raça Nova Zelândia Branco, submetidos à criação de um defeito parcial de traqueia na região ventral do 4º ao 8º anel traqueal. Os animais foram subdivididos (n=4) de acordo com o momento das eutanásias, programadas com intervalo de sete (G7), quinze (G15), trinta (G30) e sessenta dias (G60). A análise histopatológica por hematoxilina e eosina (HE) revelou que o implante 3D de PCTPE apresentou reação do tipo corpo estranho e a inflamação persistiu até os 60 dias de avaliação. A histoquímica de picrosirius vermelho revelou tendência de maior quantidade de colágeno tipo I nos tempos iniciais de inflamação, enquanto o colágeno tipo III foi predominante nos períodos tardios de avaliação. Pode-se sugerir reação exacerbada e não adequada da interface tecido-material e perpetuação do processo inflamatório. clínico reconstrutiva Entretanto regenerativa Objetivouse Objetivou se (PCTPE D 1 Branco º traqueal n=4 n4 n 4 (n=4 eutanásias G7, G7 G , (G7) G15, G15 (G15) G30 (G30 G60. G60 . (G60) HE (HE 6 avaliação Podese Pode tecidomaterial tecido inflamatório n= (n= (G7 G1 (G15 G3 (G3 G6 (G60 (n (G (G1 (G6

ABSTRACT MOS (soil organic matter) is a natural carbon reservoir, and divided into different stages of decomposition according to its solubility and structural complexity. In view of this context, this work aimed to quantify the carbon content in the humic fractions of the soil organic matter under an agroforestry system in three toposequences in the swamp of altitude in Pernambuco, Brazil. The study was carried out on the Yaguara farm, and the studied areas were native forest and three toposequences of agroforestry system. Soil samples were collected in trenches 1.5 x 1.5 m, at depths 0-20, 20-40 and 40-60 cm, with four replications. The carbon concentrations in the humic fractions of the soil decreased with increasing depth. The area with agroforestry system had the highest concentrations of carbon in the humic fractions. The stocks of humic fractions found in the agroforestry system area showed higher average values in the top area 5.62, 9.72 and 22.53 Mg ha-1 in relation to native forest 4.84, 8.28 and 19.20 Mg ha-1, respectively for fulvic acid, humic acid and humine. Among the evaluated areas, the soil with agroforestry system top area has great potential to increase the carbon storage in the humic fractions of the soil. reservoir complexity context Pernambuco Brazil farm 15 1 5 1. m 020, 020 0 20, 20 0-20 2040 40 20-4 4060 60 40-6 cm replications depth 562 62 5.62 972 9 72 9.7 2253 22 53 22.5 ha1 ha ha- 484 4 84 4.84 828 8 28 8.2 1920 19 19.2 ha1, 1, humine 02 2 0-2 204 20- 406 6 40- 56 5.6 97 7 9. 225 22. 48 4.8 82 8. 192 19. 0- 5. 4.

RESUMO A MOS (matéria orgânica do solo) é um reservatório natural de carbono e dividida em diferentes estágios de decomposição conforme sua solubilidade e complexidade estrutural. Diante desse contexto, este trabalho teve como objetivo quantificar os teores de carbono nas frações húmicas da matéria orgânica do solo sob sistema agroflorestal em três topossequências no brejo de altitude de Pernambuco, Brasil. O estudo foi desenvolvido na fazenda Yaguara, e as áreas estudadas foram mata nativa e três topossequências de sistema agroflorestal. As amostras de solos foram coletadas em trincheiras 1,5 x 1,5 m, nas profundidades 0-20, 20-40 e 40-60 cm, com quatro replicações. As concentrações de carbono nas frações húmicas do solo diminuíram com o aumento da profundidade. A área com sistema agroflorestal teve as maiores concentrações de carbono nas frações húmicas. Os estoques das frações húmicas encontradas em área de sistema agroflorestal apresentaram maiores valores médios em área de topo 5,62, 9,72 e 22,53 Mg ha-1 em relação à mata nativa 4,84, 8,28 e 19,20 Mg ha-1, respectivamente para ácido fúlvico, ácido húmico e humina. Dentre as áreas avaliadas, o solo com sistema agroflorestal área de topo tem grande potencial para aumentar o armazenamento de carbono nas frações húmicas do solo. estrutural contexto Pernambuco Brasil Yaguara 15 1 5 1, m 020, 020 0 20, 20 0-20 2040 40 20-4 4060 60 40-6 cm replicações profundidade 562 62 5,62 972 9 72 9,7 2253 22 53 22,5 ha1 ha ha- 484 4 84 4,84 828 8 28 8,2 1920 19 19,2 ha1, fúlvico humina avaliadas 02 2 0-2 204 20- 406 6 40- 56 5,6 97 7 9, 225 22, 48 4,8 82 8, 192 19, 0- 5, 4,

Abstract Because of the exacerbated use of pesticides and their mixtures, and the difficulty of evaluating their toxicity, it is necessary to identify sensitive tools for the early detection of potential environmental risks related to the use of these chemicals. Our objective was therefore to evaluate the effects of these chemicals using a rapid 24 h toxicity test in zebrafish embryos. After exposure of individual and mixtures of the pesticides carbendazim (carb), fipronil (fipr), and sulfentrazone (sulf), the lethality and sublethally at 24 hpf, the effects of the mixtures on epiboly and on coagulation of the embryos at 8 hpf were evaluated. This model can be extrapolated to several vertebrate groups, including humans. All three pesticides were toxic in both exposure periods, at 24 hpf, all concentrations used affected the embryos, with lethal and sublethal effects occurring. At 8 hpf, epiboly analysis indicated that the mixtures did not potentiate the effects compared to the pesticides alone, being toxic in the order: carb > sulf > fipr > fipr + sulf > carb + sulf > carb + fipr + sulf > carb + fipr + sulf > carb + fipr. We conclude that the most toxic pesticide was sulfentrazone, since it was highly toxic to embryonic development at 24 hpf and caused both delayed epiboly and high percent clotting at 8 hpf. Thus, we see that the rapid toxicity test was effective in assessing the toxicity of pesticides and was sensitive in assessing the effects of pesticides at low concentrations. 2 carb, , (carb) fipr, (fipr) sulf, (sulf) evaluated groups humans periods occurring alone order Thus (carb (fipr (sulf

Resumo Devido ao uso exacerbado dos agrotóxicos e suas misturas e à dificuldade de avaliação de toxicidade, faz-se necessária a busca de ferramentas sensíveis para detectar precocemente potenciais riscos ambientais relacionados ao uso desses produtos químicos. Portanto, nosso objetivo foi avaliar os efeitos desses produtos usando um teste rápido de toxicidade de 24 h em embriões de zebrafish. Após exposição dos pesticidas carbendazim (carb), fipronil (fipr) e sulfentrazona (sulf) isolados e misturados, foi avaliado a letalidade e subletalidade em 24 hpf, e os efeitos na epibolia e coagulação das misturas nos embriões em 8 hpf, com este, essa metodologia pode ser extrapolada para diversos grupos de vertebrados, inclusive humanos. Os três pesticidas foram tóxicos em ambos os períodos de exposição, a 24 hpf, todas as concentrações utilizadas afetaram os embriões, ocorrendo efeitos letais e subletais. Aos 8 hpf, a análise de epibolia indicou que as misturas não potencializaram os efeitos em relação aos agrotóxicos isoladamente, sendo tóxicas na ordem: carb > sulf > fipr > fipr + sulf > carb + sulf > carb + fipr + sulf > carb + fipr + sulf > carb + fipr. Concluímos que o pesticida mais tóxico foi o sulfentrazona, uma vez que foi altamente tóxico para o desenvolvimento embrionário em 24 hpf e causou retardo na epibolia e alto percentual de coagulação em 8 hpf. Assim, vemos que o teste rápido de toxicidade foi eficaz na avaliação da toxicidade dos pesticidas e foi sensível na avaliação dos efeitos destes pesticidas em baixas concentrações. fazse faz se químicos Portanto 2 zebrafish carb, , (carb) (fipr (sulf misturados este vertebrados humanos subletais isoladamente ordem Assim (carb

Abstract Background There is a remarkable variability in the frequency of HLA-B27 positivity in patients with spondyloarthritis (SpA), which may be associated with different clinical presentations worldwide. However, there is a lack of data considering ethnicity and sex on the evaluation of the main clinical and prognostic outcomes in mixed-race populations. The aim of this study was to evaluate the frequency of HLA-B27 and its correlation with disease parameters in a large population of patients from the Brazilian Registry of Spondyloarthritis (RBE). Methods The RBE is a multicenter, observational, prospective cohort that enrolled patients with SpA from 46 centers representing all five geographic regions of Brazil. The inclusion criteria were as follow: (1) diagnosis of axSpA by an expert rheumatologist; (2) age ≥18 years; (3) classification according to ASAS axial. The following data were collected via a standardized protocol: demographic data, disease parameters and treatment historical. Results A total of 1096 patients were included, with 73.4% HLA-B27 positivity and a mean age of 44.4 (±13.2) years. Positive HLA-B27 was significantly associated with male sex, earlier age at disease onset and diagnosis, uveitis, and family history of SpA. Conversely, negative HLA-B27 was associated with psoriasis, higher peripheral involvement and disease activity, worse quality of life and mobility. Conclusions Our data showed that HLA-B27 positivity was associated with a classic axSpA pattern quite similar to that of Caucasian axSpA patients around the world. Furthermore, its absence was associated with peripheral manifestations and worse outcomes, suggesting a relevant phenotypic difference in a highly miscegenated population. HLAB27 HLAB HLA B27 B HLA-B2 SpA, , (SpA) worldwide However mixedrace mixed race populations RBE. . (RBE) multicenter observational 4 Brazil follow 1 (1 rheumatologist 2 (2 18 ≥1 years 3 (3 axial protocol historical 109 included 734 73 73.4 444 44 44. ±13.2 132 13 (±13.2 uveitis Conversely psoriasis activity mobility world Furthermore HLAB2 B2 HLA-B (SpA (RBE ( ≥ 10 7 73. ±13. (±13. ±13 (±13 ±1 (±1 ± (±

ABSTRACT Purpose Oropharyngeal dysphagia (OD) is one of the possible outcomes in patients hospitalized with COVID-19 and also in the population hospitalized for the treatment of cardiovascular disease. Thus, knowing the predictive risk factors for OD may help with referral and early intervention. This study aimed to verify the association of different factors with OD in hospitalized individuals with cardiovascular disease and COVID-19. Methods Cross-sectional clinical study approved by the Research Ethics Committee (4,521,771). Clinical evaluation of swallowing was carried out in 72 adult patients with cardiovascular disease and COVID-19 hospitalized from April to September 2020. Individuals under 18 years of age and without previous cardiovascular disease were excluded. The presence of general clinical and/or neurological complications, pronation, stay in the intensive care unit (ICU), orotracheal intubation (OTI), tracheostomy tube, oxygen support and age were considered as predictive risk factors for oropharyngeal dysphagia. Fisher's exact test, Mann Whitney test and logistic regression model were used for analysis. Results General clinical complications (p=0.001), pronation (p=0.003), ICU stay (p=0.043), in addition to the need for oxygen supplementation (p=0.023) and age (p= 0 .037) were statistically significant factors associated. The pronation (0.013) and age (0.038) were independently associated with dysphagia. OTI (p=0.208), tracheostomy (p=0.707) and the presence of previous cerebrovascular accidents (p=0.493) were not statistically significant. Conclusion In this study, age and prone position were factors independently associated with oropharyngeal dysphagia, complications such as the need for oxygen supplementation, in addition to the need for ICU admission, were also associated factors in the population. (OD COVID19 COVID 19 COVID-1 Thus intervention COVID19. 19. Crosssectional Cross sectional 4,521,771. 4521771 4,521,771 . 4 521 771 (4,521,771) 7 2020 1 excluded andor or ICU, , (ICU) OTI, (OTI) tube Fishers Fisher s analysis p=0.001, p0001 p p=0.001 001 (p=0.001) p=0.003, p0003 p=0.003 003 (p=0.003) p=0.043, p0043 p=0.043 043 (p=0.043) p=0.023 p0023 023 (p=0.023 p= (p .037 037 0.013 0013 013 (0.013 0.038 0038 038 (0.038 p=0.208, p0208 p=0.208 208 (p=0.208) p=0.707 p0707 707 (p=0.707 p=0.493 p0493 493 (p=0.493 admission COVID1 COVID- 452177 4,521,77 52 77 (4,521,771 202 (ICU (OTI p000 p=0.00 00 (p=0.001 (p=0.003 p004 p=0.04 04 (p=0.043 p=0.02 p002 02 (p=0.02 .03 03 0.01 01 (0.01 0.03 (0.03 p020 p=0.20 20 (p=0.208 p=0.70 p070 70 (p=0.70 p=0.49 p049 49 (p=0.49 45217 4,521,7 5 (4,521,77 p00 p=0.0 (p=0.00 (p=0.04 (p=0.0 .0 0.0 (0.0 p02 p=0.2 2 (p=0.20 p=0.7 p07 (p=0.7 p=0.4 p04 (p=0.4 4521 4,521, (4,521,7 p0 p=0. (p=0. 0. (0. (p=0.2 452 4,521 (4,521, p=0 (p=0 (0 45 4,52 (4,521 ( 4,5 (4,52 4, (4,5 (4, (4

ABSTRACT Objective: To analyze the spatial flow of care for patients undergoing dialysis therapy in the health regions of the State of Minas Gerais. Methods: Ecological study whose population was patients undergoing dialysis therapy in public, philanthropic institutions or whose treatment was paid for by the Unified Health System in private clinics in partnership, in the State of Minas Gerais. Patients were grouped by health region of residence. The proportions of patients who underwent dialysis were calculated, as well as enrollment on the kidney transplant list in their own region of residence or outside it. Person correlations of these proportions with socioeconomic and care indicators of the health regions were estimated. Spatial exploratory techniques estimated general (Moran’s I) and local (LISA) spatial correlation coefficients. Results: Regions with higher GDP had a higher number of nephrologists and a higher proportion of registrations in the region of residence. A cluster of regions with low GDP was identified further to the northeast of the State (also with lower nephrologist ratio values), a cluster with a high proportion of those registered on the transplant list in the center of the State, and a cluster with a low proportion of dialysis in the same region of residence further southeast. Conclusion: Regional disparities were evident in relation to the proportion of patients registered on the waiting list for kidney transplantation, the proportion of patients undergoing dialysis in the same region of residence and the proportion of patients registered on the waiting list for kidney transplantation in the same region of residence. residence. Objective Gerais Methods public partnership calculated it Morans Moran s I LISA (LISA coefficients Results also values, values , values) southeast Conclusion

RESUMO Objetivo: Analisar o fluxo espacial da assistência de pacientes em terapia dialítica nas regionais de saúde do estado de Minas Gerais. Métodos: Estudo ecológico que teve como população pacientes incidentes em terapia dialítica em instituições públicas, filantrópicas ou que tiveram seu tratamento custeado pelo Sistema Único de Saúde em clínicas privadas conveniadas, no estado de Minas Gerais. Os pacientes foram agregados por regional de saúde de residência. Foram calculadas as proporções de pacientes que fizeram diálise, bem como a inscrição na lista de transplante renal em sua própria região de residência ou fora dela. Estimadas as correlações de Person destas proporções com indicadores socioeconômicos e assistenciais das regionais de saúde. Técnicas exploratórias espaciais estimaram coeficientes de correlação espacial geral (I de Moran) e local (LISA). Resultados: Regiões com maior PIB apresentaram maior razão de nefrologistas e maior proporção de inscrições na própria região de residência. Identificou-se um cluster de regiões com PIB baixo mais ao nordeste do estado (também com valores mais baixos de razão de nefrologistas), um cluster de alta proporção de inscritos na lista de transplante no centro do estado, e um cluster de baixa proporção de diálise na mesma região de residência mais ao sudeste. Conclusão: Evidenciou-se disparidades regionais em relação à proporção de inscritos na lista de espera para o transplante renal, proporção de pacientes que realizavam diálise na mesma região de residência e proporção de pacientes inscritos na lista de espera para o transplante renal na mesma região de residência. Objetivo Gerais Métodos públicas conveniadas dela I Moran LISA. LISA . (LISA) Resultados Identificouse Identificou se também nefrologistas, , nefrologistas) sudeste Conclusão Evidenciouse Evidenciou (LISA