ABSTRACT Purpose: Acute-on-chronic liver failure (ACLF) is a leading cause of death in cirrhotic patients. This study aims to describe the outcomes of in-patients with ACLF at a liver transplantation (LT) center in Brazil. Methods: Retrospective study analyzing patient data from 2017 to 2022. Re-transplant cases and patients without previous chronic liver disease were excluded. The ACLF diagnosis was based on the European Association for the Study of the Liver-Chronic Liver Failure criteria and assessments repeated on days 3 and 7 after the initial diagnosis. Results: Among 381 patients, 10.49% (n = 40) were diagnosed with ACLF. Bacterial infection was the most common precipitating factor (45%). Kidney failure occurred in 65% of the cases. The 28-day mortality rate was 35% and varied according to ACLF severity at diagnosis, from single organ failure (ACLF-1) at 22% to three organ failures (ACLF-3) at 60%. Eighteen patients (45%) were transplanted with a 100% 28-day survival rate. For ACLF-3 cases at diagnosis (n = 15), the 28-day and 1-year survival rates with a transplant (n = 4) were 100% and 80%, respectively, and without transplant (n = 11), 10 and 0%, respectively. Conclusions: ACLF was associated with high mortality rates. LT was an effective therapeutic option, particularly for ACLF-3 cases. Purpose Acuteonchronic Acute (ACLF inpatients (LT Brazil Methods 201 2022 Retransplant Re excluded LiverChronic Chronic Results 38 1049 49 10.49 n 40 45%. 45 45% . 65 28day day 28 35 ACLF1 1 (ACLF-1 22 ACLF3 (ACLF-3 60 60% (45% 100 ACLF- 15, 15 , 15) 1year year 4 80 80% respectively 11, 11 11) 0 0% Conclusions option 20 202 104 10.4 6 2 (ACLF- (45 8 10. (4 (

Resumo Objetivo Identificar a prevalência do uso de drogas lícitas e ilícitas por gestantes que realizavam pré-natal na Atenção Primária à Saúde e fatores associados ao seu uso. Métodos Estudo transversal realizado por meio de roteiro estruturado, composto de variáveis sociodemográficas gestacionais e uso de drogas pela gestante e seus familiares, aplicado a 270 mulheres em pré-natal de baixo risco de 15 Unidades Básicas de Saúde, de dois municípios de uma região metropolitana do Sul do Brasil, onde existem diferenças socioeconômicas e demográficas negativas em relação a cidade-polo. A coleta de dados ocorreu no período de dezembro de 2019 a fevereiro de 2020. Os dados foram analisados por meio do teste do qui-quadrado de Pearson, sendo consideradas significativas as associações com valor de p<0,05. Resultados Jovens de 25 a 35 anos corresponderam a 77% da amostra; pardas/negras foram 60,4%; multigestas somaram 41,8% (com mais de três filhos). Tinham parceiro conjugal fixo 86,6%. A prevalência de uso atual de drogas foi de 46,2%. Houve associação estatisticamente significativa entre consumo de álcool e ter tido intercorrências gestacionais (razão de chance de 2,5; intervalo de confiança de 1,17-5,22); entre o consumo de maconha e a idade de 15 a 19 anos (razão de chance de 2,7; intervalo de confiança de 1,01- 7,03); entre consumo de tabaco e uso de drogas pelo esposo (razão de chance de 4,1; intervalo de confiança de 1,75- 9,55) e entre uso de tabaco e classificação familiar do tipo monoparental (razão de chance de 6,6; intervalo de confiança de 1,55 - 28,43). Conclusão A prevalência entre uso atual e uso na vida foi elevada. As gestantes eram multigestas em pré-natal de baixo risco. O uso de drogas esteve associado a intercorrências gestacionais, idade, uso de drogas pelo esposo e a classificação familiar do tipo monoparental. prénatal pré natal estruturado familiares 27 1 Brasil cidadepolo. cidadepolo cidade polo. polo cidade-polo 201 2020 quiquadrado qui quadrado Pearson p005 p 0 05 p<0,05 2 3 77 amostra pardasnegras pardas negras 60,4% 604 60 4 418 41 8 41,8 filhos. filhos . filhos) 866 86 6 86,6% 462 46 46,2% razão 2,5 5 1,175,22 117522 1,17 5,22 17 22 1,17-5,22) 2,7 7 1,01 101 01 7,03 703 03 7,03) 4,1 1,75 175 75 9,55 955 9 55 6,6 66 155 1,5 28,43. 2843 28,43 28 43 28,43) elevada 20 202 p00 p<0,0 60,4 41, 86,6 46,2 2, 1,175,2 11752 117 1,1 522 5,2 1,17-5,22 1,0 10 7,0 70 4, 1,7 9,5 95 6, 1, 284 28,4 p0 p<0, 60, 86, 46, 1,175, 1175 11 52 5, 1,17-5,2 7, 9, 28, p<0 1,175 1,17-5, p< 1,17-5 1,17-

Abstract Objective To identify the prevalence of the use of legal and illicit drugs by pregnant women who received prenatal care in Primary Health Care and factors associated with their usage. Methods Cross-sectional study carried out using a structured script, composed of gestational sociodemographic variables and drug use by pregnant women and their families, applied to 270 women undergoing low-risk prenatal care at 15 Basic Health Units, in two municipalities in a metropolitan region of Southern Brazil, where there are negative socioeconomic and demographic differences in relation to the hub city. Data collection took place from December 2019 to February 2020. Data were analyzed using Pearson’s chi-square test, with associations with a value of p<0.05 being considered significant. Results Young people aged 25 to 35 accounted for 77% of the sample; brown/black were 60.4%; multigravidae accounted for 41.8% (with more than three children). 86.6% had a steady marital partner. The prevalence of current drug use was 46.2%. There was a statistically significant association between alcohol consumption and having had pregnancy complications (odds ratio of 2.5; confidence interval of 1.17-5.22); between marijuana consumption and the age of 15 to 19 years (odds ratio of 2.7; confidence interval of 1.01-7.03); between tobacco consumption and drug use by the husband (odds ratio of 4.1; confidence interval of 1.75-9.55) and between tobacco use and single-parent family classification (odds ratio of 6.6 ; confidence interval 1.55 - 28.43). Conclusion The prevalence between current usage and lifetime use was high. The multipregnancies in women were undergoing low-risk prenatal care. Drug use, age, drug use by the husband and single-parent family classification were associated with gestational complications. Crosssectional Cross sectional script families 27 lowrisk low risk 1 Units Brazil city 201 2020 Pearsons Pearson s chisquare chi square test p005 p 0 05 p<0.0 2 3 77 sample brownblack brown black 60.4% 604 60 4 418 41 8 41.8 children. children . children) 866 86 6 86.6 partner 462 46 46.2% odds 2.5 5 1.175.22 117522 1.17 5.22 17 22 1.17-5.22) 2.7 7 1.017.03 101703 1.01 7.03 01 03 1.01-7.03) 4.1 1.759.55 175955 1.75 9.55 75 9 55 1.75-9.55 singleparent single parent 66 6. 155 1.5 28.43. 2843 28.43 28 43 28.43) high 20 202 p00 p<0. 60.4 41. 86. 46.2 2. 175 1.175.2 11752 117 1.1 522 5.2 1.17-5.22 017 1.017.0 10170 101 1.0 703 7.0 1.01-7.03 4. 759 1.759.5 17595 1.7 955 9.5 1.75-9.5 1. 284 28.4 p0 p<0 60. 46. 1.175. 1175 11 52 5. 1.17-5.2 1.017. 1017 10 70 7. 1.01-7.0 1.759. 1759 95 9. 1.75-9. 28. p< 1.175 1.17-5. 1.017 1.01-7. 1.759 1.75-9 1.17-5 1.01-7 1.75- 1.17- 1.01-

Resumen Objetivo Identificar la prevalencia del consumo de drogas lícitas e ilícitas por mujeres embarazadas que realizaban control prenatal en la Atención Primaria de Salud y factores asociados a su uso. Métodos Estudio transversal realizado por medio de guion estructurado, compuesto por variables sociodemográficas gestacionales y el uso de drogas por parte de la mujer embarazada y sus familiares, aplicado a 270 mujeres en control prenatal de bajo riesgo de 15 Unidades Básicas de Salud, de dos municipios de una región metropolitana del sur de Brasil, donde existen diferencias socioeconómicas y demográficas negativas con relación a la ciudad principal. La recopilación de datos se llevó a cabo en el período de diciembre de 2019 a febrero de 2020. Los datos se analizaron mediante la prueba χ2 de Pearson, donde se consideraron significativas las relaciones con valor de p<0,05. Resultados Jóvenes de 25 a 35 años compusieron el 77 % de la muestra. El 60,4 % era parda/negra. El 41,8 % era multigesta (con más de tres hijos). El 86,6 % tenían pareja conyugal. La prevalencia de consumo actual de drogas fue del 46,2 %. Hubo asociación estadísticamente significativa entre el consumo de alcohol y haber tenido complicaciones gestacionales (razón de momios de 2,5; intervalo de confianza de 1,17-5,22); entre el consumo de marihuana y la edad de 15 a 19 años (razón de momios de 2,7; intervalo de confianza de 1,01-7,03); entre el consumo de tabaco y el consumo de drogas por parte del esposo (razón de momios de 4,1; intervalo de confianza de 1,75-9,55) y entre el consumo de tabaco y la clasificación familiar monoparental (razón de momios de 6,6; intervalo de confianza de 1,55-28,43). Conclusión La prevalencia entre el consumo actual y el consumo en la vida fue elevada. Las mujeres embarazadas eran multigestas con control prenatal de bajo riesgo. El consumo de drogas estuvo asociado a complicaciones gestacionales, edad, consumo de drogas por parte del esposo y la clasificación familiar monoparental. estructurado familiares 27 1 Brasil principal 201 2020 χ Pearson p005 p 0 05 p<0,05 2 3 7 muestra 604 60 4 60, pardanegra parda negra parda/negra 418 41 8 41, hijos. hijos . hijos) 866 86 6 86, conyugal 462 46 46, razón 2,5 5 1,175,22 117522 1,17 5,22 17 22 1,17-5,22) 2,7 1,017,03 101703 1,01 7,03 01 03 1,01-7,03) 4,1 1,759,55 175955 1,75 9,55 75 9 55 1,75-9,55 6,6 66 1,5528,43. 1552843 1,55 28,43 28 43 1,55-28,43) elevada 20 202 p00 p<0,0 2, 175 1,175,2 11752 117 1,1 522 5,2 1,17-5,22 017 1,017,0 10170 101 1,0 703 7,0 1,01-7,03 4, 759 1,759,5 17595 1,7 955 9,5 1,75-9,5 6, 5528 1,5528,43 155284 155 1,5 2843 28,4 1,55-28,43 p0 p<0, 1,175, 1175 11 1, 52 5, 1,17-5,2 1,017, 1017 10 70 7, 1,01-7,0 1,759, 1759 95 9, 1,75-9, 552 1,5528,4 15528 284 28, 1,55-28,4 p<0 1,175 1,17-5, 1,017 1,01-7, 1,759 1,75-9 1,5528, 1552 1,55-28, p< 1,17-5 1,01-7 1,75- 1,5528 1,55-28 1,17- 1,01- 1,552 1,55-2 1,55-

Objective: To ascertain whether sociodemographic and health-related characteristics known from previous research to have a substantive impact on recovery from depression modified the effect of a digital intervention designed to improve depressive symptoms (CONEMO). Methods: The CONEMO study consisted of two randomized controlled trials, one conducted in Lima, Peru, and one in São Paulo, Brazil. As a secondary trial plan analysis, mixed logistic regression was used to explore interactions between the treatment arm and subgroups of interest defined by characteristics measured before randomization – suicidal ideation, race/color, age, gender, income, type of mobile phone, alcohol misuse, tobacco use, and diabetes/hypertension – in both trials. We estimated interaction effects between the treatment group and these subgroup factors for the secondary outcomes using linear mixed regression models. Results: Increased effects of the CONEMO intervention on the primary outcome (reduction of at least 50% in depressive symptom scores at 3-month follow-up) were observed among older and wealthier participants in the Lima trial (p = 0.030 and p = 0.001, respectively). Conclusion: There was no evidence of such differential effects in São Paulo, and no evidence of impact of any other secondary outcomes in either trial. Clinical trial registration: NCT02846662 (São Paulo, Brazil – SP), NCT03026426 (Lima, Peru – LI). Funded by the U.S. National Institute of Mental Health (grant U19MH098780). Objective healthrelated health related CONEMO. . (CONEMO) Methods trials Paulo analysis ideation racecolor race color race/color age gender income phone misuse use diabeteshypertension diabetes hypertension models Results reduction 50 3month month 3 followup follow up follow-up 0030 0 030 0.03 0001 001 0.001 respectively. respectively respectively) Conclusion registration NCT NCT0284666 SP, SP , SP) NCT0302642 (Lima LI. LI LI) US U S U.S grant U19MH098780. U19MH098780 UMH MH U19MH098780) (CONEMO 5 003 03 0.0 000 00 0.00 NCT028466 NCT030264 U19MH09878 0. NCT02846 NCT03026 U19MH0987 NCT0284 NCT0302 U19MH098 NCT028 NCT030 U19MH09 NCT02 NCT03 U19MH0 NCT0 U19MH

Resumo O objetivo deste estudo é conhecer os fluxos econômico-ecológicos dos Sistemas Agroflorestais Sucessionais (SAFS) em agroecossistemas agroecológicos na região da Serra dos Tapes (RS). Para isso foi utilizado o método LUME: Avaliação Econômico-Ecológica de Agroecossistemas desenvolvido pela Assessoria e Serviços a Projetos em Agricultura Alternativa (AS-PTA), a partir de suas experiências acumuladas desde 1989. Este método fornece um aparato teórico-metodológico e analítico de modelo misto (quali-quantitativo). Com esta ferramenta, foram estudados três agroecossistemas agroecológicos que produzem em SAFS há mais de 10 anos. Dentre os principais resultados foi observado que os mesmos movimentam até 126 fluxos econômico-ecológicos e que os Sistemas Agroflorestais Sucessionais da Serra dos Tapes (SAFST) são subsistemas em expansão, apresentando capacidade e diversificação produtiva. Conclui-se que os SAFST são multifuncionais, ou seja, cumprem sua função primária de produzir alimentos, além de aumentar a biodiversidade e melhorar a condição socioeconômica dos locais estudados, fomentando mercados territoriais e sendo uma importante ferramenta para a reprodução ampliada da vida no contexto da agricultura familiar agroecológica desta região. econômicoecológicos econômico ecológicos (SAFS RS. RS . (RS) LUME EconômicoEcológica Econômico Ecológica ASPTA, ASPTA AS PTA , (AS-PTA) 1989 teóricometodológico teórico metodológico qualiquantitativo. qualiquantitativo quali quantitativo (quali-quantitativo) 1 anos 12 (SAFST expansão produtiva Concluise Conclui se multifuncionais seja alimentos (RS (AS-PTA 198 (quali-quantitativo 19

Abstract The objective of this study is to understand the economic-ecological flows of Successional Agroforestry Systems (SAFS) in agroecological agroecosystems in the Serra dos Tapes region (RS). For this purpose, the LUME method was used: Economic-Ecological Assessment of Agroecosystems developed by the Advisory and Services for Projects in Alternative Agriculture (AS-PTA), based on its experiences accumulated since 1989. This method provides a theoretical-methodological and analytical apparatus for mixed model (quali-quantitative). With this tool, three agroecological agroecosystems that have been producing in SAFS for more than 10 years were studied. Among the main results, it was observed that they move up to 126 economic-ecological flows and that the Serra dos Tapes Successive Agroforestry Systems (SAFST) are expanding subsystems, presenting capacity and productive diversification. It is concluded that SAFST are multifunctional, that is, they fulfill their primary function of producing food, in addition to increasing biodiversity and improving the socioeconomic condition of the places studied, promoting territorial markets and being an important tool for the expanded reproduction of life in the context of agroecological family farming in this region. economicecological economic ecological (SAFS RS. RS . (RS) purpose used EconomicEcological Economic Ecological ASPTA, ASPTA AS PTA , (AS-PTA) 1989 theoreticalmethodological theoretical methodological qualiquantitative. qualiquantitative quali quantitative (quali-quantitative) 1 studied results 12 (SAFST subsystems diversification multifunctional food (RS (AS-PTA 198 (quali-quantitative 19

Resumen El objetivo de este estudio es comprender los flujos económico-ecológicos de los Sistemas Agroforestales de Sucesión (SAFS) en agroecosistemas agroecológicos de la región de la Serra dos Tapes (RS). Para ello se utilizó el método LUME: Evaluación Económico-Ecológica de Agroecosistemas desarrollado por la Asesoría y Servicios para Proyectos de Agricultura Alternativa (AS-PTA), con base en sus experiencias acumuladas desde 1989. Este método proporciona modelo mixto (cuali-cuantitativo) de aparato teórico-metodológico y analítico. Con esta herramienta se estudiaron tres agroecosistemas agroecológicos que producen en SAFS hace más de 10 años. Entre los principales resultados, se observó que movilizan hasta 126 flujos económico-ecológicos y que los Sistemas Sucesivos Agroforestales de la Serra dos Tapes (SAFST) son subsistemas en expansión, presentando capacidad y diversificación productiva. Se concluye que los SAFST son multifuncionales, es decir, cumplen su función primordial de producir alimentos, además de incrementar la biodiversidad y mejorar la condición socioeconómica de los lugares estudiados, promoviendo los mercados territoriales y siendo una importante herramienta para la reproducción ampliada de la vida en el contexto de la agricultura familiar agroecológica en esta región. económicoecológicos económico ecológicos (SAFS RS. RS . (RS) LUME EconómicoEcológica Económico Ecológica ASPTA, ASPTA AS PTA , (AS-PTA) 1989 cualicuantitativo cuali cuantitativo (cuali-cuantitativo teóricometodológico teórico metodológico analítico 1 años resultados 12 (SAFST expansión productiva multifuncionales decir alimentos estudiados (RS (AS-PTA 198 19

In this study, electrospun nanofibers of poly(vinylidene fluoride) (PVDF) were produced with the addition of [6,6]-phenyl-C61-butyric acid methyl ester (PCBM) and poly(3-hexylthiophene) (P3HT), for application as a nanostructured active layer of bulk heterojunction polymer with fullerene and polythiophene derivatives in third-generation photovoltaic devices. The electrospinning technique was used to favor the formation of the β phase, exhibiting ferroelectric properties, improving the electrical performance of the device. The morphological, structural and electrical characteristics of the nanofibers were investigated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and DC electrical measurements. The synergism between nanofiber processing and the addition of P3HT and PCBM improved electrical conductivity by up to three orders of magnitude, furthermore, the nanofibers incorporating P3HT exhibited responses to solar stimuli. study polyvinylidene poly vinylidene fluoride PVDF (PVDF 6,6phenylC61butyric 66phenylC61butyric phenylCbutyric 6,6 phenyl C61 butyric 6 C (PCBM poly3hexylthiophene polyhexylthiophene 3 hexylthiophene poly(3-hexylthiophene P3HT, PHT , P HT (P3HT) thirdgeneration third generation devices phase properties device morphological SEM, SEM (SEM) FTIR (FTIR measurements magnitude furthermore stimuli 6phenylC61butyric phenylC 66 6, C6 (P3HT (SEM

RESUMO Objetivo: Avaliar, de forma retrospectiva, a citação do ligamento anterolateral (LAL) em laudos de ressonância magnética (RM) e confirmar sua presença e lesão nas imagens de exames feitos na fase aguda. Métodos: Foram incluídos 103 pacientes submetidos à reconstrução do ligamento cruzado anterior (LCA) em 2019, cujas imagens foram reanalisadas por dois radiologistas. Resultados: Em primeira análise, apenas um laudo citava o LAL e sua lesão (0,97%); enquanto na reanálise, o LAL foi visualizado em quase todos os casos (95% pelo radiologista A e 97% pelo radiologista B). Foi encontrada lesão em 53 (51,5%) casos pelo radiologista A e 56 (54,4%) pelo radiologista B. Lesão foi diagnosticada por ambos em 39 (37,9%) casos (p < 0,0001). Houve divergência entre os radiologistas com relação à lesão (KappaL = 0,411). Conclusão: Os laudos deixaram de descrever o ligamento e diagnosticar um número significativo de lesões. A análise das imagens convencionais de ressonância ainda gera divergências no diagnóstico da lesão do LAL associada ao LCA entre os radiologistas. Nível de Evidência IV, Série de Casos. Objetivo Avaliar retrospectiva (LAL RM (RM aguda Métodos 10 (LCA 2019 Resultados 0,97% 097 0 97 (0,97%) reanálise 95% 95 (95 B . B) 5 51,5% 515 51 (51,5% 54,4% 544 54 4 (54,4% 3 37,9% 379 37 9 (37,9% p 0,0001. 00001 0,0001 0001 0,0001) KappaL 0,411. 0411 0,411 411 0,411) Conclusão lesões IV Casos 1 201 0,97 09 (0,97% (9 51,5 (51,5 54,4 (54,4 37,9 (37,9 0000 0,000 000 041 0,41 41 20 0,9 (0,97 ( 51, (51, 54, (54, 37, (37, 0,00 00 04 0,4 2 0, (0,9 (51 (54 (37 0,0 (0, (5 (3 (0

ABSTRACT Objective: To evaluate the citation of the ligament in the magnetic resonance imaging (MRI) reports and confirm its presence and injury in the images of exams performed in the acute phase retrospectively. Methods: In total, 103 patients who underwent anterior cruciate ligament (ACL) reconstruction in 2019 were included. The images were reanalyzed by two radiologists. Results: In the first analysis, only one report mentioned the anterolateral ligament (ALL) and its injury (0.97%). On reanalysis, ALL was visualized in almost all cases (95% and 97%). An injury was found in 53 (51.5%) cases by radiologist A and in 56 (54.4%) cases by radiologist B. The injury was diagnosed by both in 39 (37.9%) cases (p < 0.0001). Radiologists disagreed regarding the injury (Kappa = 0.411). Conclusion: The reports failed to describe the ligament and diagnose a significant number of injuries. The analysis of conventional resonance images still presents divergences in the diagnosis of ALL injury associated with the ACL among radiologists. Level of Evidence IV, Case Series. Objective MRI (MRI retrospectively Methods total 10 (ACL 201 included radiologists Results (ALL 0.97%. 097 0.97% . 0 97 (0.97%) reanalysis 95% 95 (95 97%. 97% 97%) 5 51.5% 515 51 (51.5% 54.4% 544 54 4 (54.4% B 3 37.9% 379 37 9 (37.9% p 0.0001. 00001 0.0001 0001 0.0001) Kappa 0.411. 0411 0.411 411 0.411) Conclusion injuries IV Series 1 20 09 0.97 (0.97% (9 51.5 (51.5 54.4 (54.4 37.9 (37.9 0000 0.000 000 041 0.41 41 2 0.9 (0.97 ( 51. (51. 54. (54. 37. (37. 0.00 00 04 0.4 0. (0.9 (51 (54 (37 0.0 (0. (5 (3 (0

Resumo Introdução: Diabetes mellitus (DM) é uma doença crônica caracterizada por hiperglicemia levando à nefropatia diabética (ND). Mostramos que P2X7, um receptor purinérgico, foi altamente expresso na DM; entretanto, quando o estresse oxidativo foi controlado, o NO renal recuperou-se, e a ativação deste receptor permaneceu significativamente reduzida. Este estudo objetivou avaliar a influência do NO no P2X7 e a apoptose em células mesangiais imortalizadas de camundongos (CMiC) cultivadas em meio de glicose elevada (GE). Métodos: CMiCs foram cultivadas em meio DMEM e expostas à glicose normal (GN), manitol (MA), ou GE. A viabilidade celular foi avaliada por contador automático. Sobrenadantes foram coletados para quantificação de NO, e foram extraídas proteínas para análise de NO sintases (iNOS e eNOS), caspase-3, e P2X7. Resultados: A viabilidade celular permaneceu acima de 90% em todos os grupos. Houve aumento significativo na proliferação de células na GE comparado com MA e GN. NO, iNOS, caspase-3 e P2X7 foram significativamente aumentados na GE comparados com GN e MA, sem alterações na eNOS. Observamos que houve correlação forte e significativa entre P2X7 e NO. Discussão: O principal achado foi que a produção de NO pela iNOS foi positivamente correlacionada com aumento de P2X7 em CMs sob condições de GE, mostrando que existe um estímulo comum entre eles e que o NO interage com a via do P2X7, contribuindo para apoptose na DM experimental. Estes achados podem ser relevantes para estudos de alvos terapêuticos para a prevenção e/ou tratamento de danos renais induzidos por hiperglicemia para retardar a progressão da ND.

Abstract Introduction: Diabetes mellitus (DM) is a chronic disease characterized by hyperglycemia that leads to diabetic nephropathy (DN). We showed that P2X7, a purinergic receptor, was highly expressed in DM; however, when oxidative stress was controlled, renal NO recovered, and the activation of this receptor remained significantly reduced. The aim of this study was to assess the influence of NO on the P2X7 and apoptosis in mouse immortalized mesangial cells (MiMC) cultured in high glucose (HG) medium. Methods: MiMCs were cultured with DMEM and exposed to normal glucose (NG), mannitol (MA), or HG. Cell viability was assessed by an automated counter. Supernatants were collected for NO quantification, and proteins were extracted for analysis of NO synthases (iNOS and eNOS), caspase-3, and P2X7. Results: Cell viability remained above 90% in all groups. There was a significant increase in the proliferation of cells in HG compared to MA and NG. NO, iNOS, caspase-3, and P2X7 were significantly increased in HG compared to NG and MA, with no changes in eNOS. We observed that there was a strong and significant correlation between P2X7 and NO. Discussion: The main finding was that the production of NO by iNOS was positively correlated with the increase of P2X7 in MCs under HG conditions, showing that there is a common stimulus between them and that NO interacts with the P2X7 pathway, contributing to apoptosis in experimental DM. These findings could be relevant to studies of therapeutic targets for the prevention and/or treatment of hyperglycemia-induced kidney damage to delay DN progression.

Resumo A composição florística dos campos rupestres compreende um mosaico de espécies com adaptações distintas, exibindo alta diversidade e endemismo. Entretanto, as plantas dos campos rupestres possuem dependência às condições específicas do local e sua reprodução quase sempre é limitada, tornando difícil a regeneração natural após perturbações. O banco de sementes do solo representa um fator potencial para a regeneração deste ambiente. Objetivou-se avaliar o banco de sementes do solo de diferentes profundidades de uma área de campo rupestre, coletado em duas estações climáticas e submetido a duas condições de luminosidade. Foram coletadas amostras compostas de serapilheira e de solo (0-5 cm) em 13 parcelas de 100 m2, em uma área de campo rupestre na Serra do Cipó (Minas Gerais), nas estações seca e chuvosa. As amostras foram submetidas às condições de luminosidade pleno sol e sombreamento. No total, emergiram 185 plântulas pertencentes a 31 espécies e a nove famílias botânicas. As famílias com maior riqueza foram Cyperaceae, Poaceae e Asteraceae. Houve variação de riqueza, abundância e composição florística entre as profundidades do solo, mas não foi observada variação em relação à estacionalidade e às condições de luminosidade. As amostras de solo apresentaram valores superiores dos parâmetros avaliados em relação àquelas da serapilheira, mostrando que o banco de sementes é mais representativo no solo. Esses resultados demonstram que existe potencial de regeneração do campo rupestre por meio do banco de sementes do solo.

Abstract The floristic composition of the rupestrian grasslands comprises a mosaic of species with different adaptations exhibiting high diversity and endemism. However, the plant species from rupestrian grasslands are dependent on site-specific conditions and their reproduction is almost always limited, making natural regeneration after disturbances difficult. The soil seed bank represents a potential factor for the regeneration of this environment. The objective was to evaluate the soil seed bank of different depths of a rupestrian grassland area, collected in two climatic seasons and submitted to different light conditions. The samples composed of litter and soil (0-5 cm) were collected in 13 plots of 100 m2, in a rupestrian grassland area in Serra do Cipó (Minas Gerais state), in dry and rainy seasons. The samples were subjected to full sun and shading conditions. In total, 185 seedlings of 31 species and nine botanical families emerged. The richest families were Cyperaceae, Poaceae and Asteraceae. There was variation in richness, abundance and floristic composition among the soil strata, but no variation was observed in relation to seasonality and light conditions. The soil samples presented higher values of the evaluated parameters in relation to those ones of litter, showing that the seed bank is more representative in the soil stratum. These results demonstrate that there is potential for rupestrian grassland regenerations through the seed bank, especially the soil stratum.

Resumo A zínia (Zinnia elegans Jacq.) é uma espécie amplamente cultivada e utilizada como planta ornamental no mercado floral mundial. Embora possua grande potencial para fitorremediação e compostos com atividades anti-infecciosas e antioxidante, poucos são os trabalhos que abordem sobre o processo de produção de suas mudas, principalmente utilizando substratos oriundos de resíduos orgânicos. Este estudo teve como objetivo avaliar o potencial do biossólido como componente de substrato no crescimento inicial de mudas de Zinnia elegans. As mudas foram produzidas em condições de viveiro com controle de luminosidade de 50%. O delineamento experimental foi inteiramente casualizado. Foram seis tratamentos, quatro repetições e cinco plantas por parcela. Os tratamentos foram constituídos por substratos resultantes da mistura de terra de subsolo (TS) e biossólido (BIO) em diferentes proporções: 100% TS (controle); 80% TS + 20% BIO; 60% TS + 40% BIO; 40% TS + 60% BIO; 20% TS + 80% BIO; T6 100% BIO. Foram avaliadas as características morfológicas e de qualidade das mudas. Realizou-se a análise de regressão polinomial e coeficiente de correlação de Pearson. Houve ajuste de regressão quadrática para altura da planta, diâmetro do coleto, área foliar, clorofila total, massa seca da parte aérea, raiz e total. A característica razão altura/diâmetro foi a que menos se correlacionou com as demais, sendo observada correlação positiva apenas com altura da planta. O uso do biossólido na proporção de 60% na composição de substrato se mostrou eficiente para o crescimento inicial de mudas, pois apresentou as melhores respostas para a maioria das características morfológicas e de qualidade das mudas.

Abstract Zinnia (Zinnia elegans Jacq.) is a widely cultivated species and used as an ornamental plant in the world flower market. Although it has great potential for phytoremediation and compounds with anti-infective and antioxidant activities there are few studies that address the process of production of its seedlings especially using substrates from organic waste. This study aimed to evaluate the potential of biosolid as a substrate component in the initial growth of Zinnia elegans seedlings. The seedlings were produced in nursery conditions with 50% light control. The experimental design was entirely randomized. There were six treatments, four repetitions and five plants per plot. The treatments consisted of substrates resulting from the mixture of subsoil (SS) and biosolid (BIO) in different proportions: 100% SS (control); 80% SS + 20% BIO; 60% SS + 40% BIO; 40% SS + 60% BIO; 20% SS + 80% BIO; T6 100% BIO. The morphological and quality characteristics of the seedlings were evaluated. Polynomial regression analysis and Pearson correlation coefficient were performed. Quadratic regression fitting was observed for plant height, stem diameter, leaf area, total chlorophyll, and shoot and root dry mass, as well as total dry mass. The shoot height/stem diameter ratio was the characteristic that correlated least with the others, being observed a positive correlation only with plant height. The use of biosolid in the proportion of 60% in the substrate composition proved to be efficient for the initial growth of seedlings as it showed the best responses for most morphological and quality characteristics of the seedlings.

ABSTRACT Given the above, the objective of this study was to evaluate the bromatological, mineral and bioactive compounds of blackberry grown in a subtropical region. Blackberries fruits (Rubus sp.), Grown in an orchard of Unioeste, Campus Marechal Cândido Rondon (Paraná, Brazil), were used. Immediately after harvest, the fruits were taken to the Food Technology Laboratory for chemical analysis and bioactive compounds. The fruit samples for the analysis of reducing, bromatological and mineral sugars were frozen and sent to private laboratories. Hybrids are more perishable than cultivars. There is variation in color among the cultivars and hybrids studied. Hybrids and cultivars of black mulberry have a high content of ascorbic acid and fibers, with emphasis on the cultivar Tupy (75.0 mg 100 mL-1 and 7.23, respectively). Higher pH is verified in the cultivars Tupy and Arapaho (3.22 and 3.24, respectively). The Arapaho cultivar has fewer acid fruits (0.25 g 100 g-1), SS/total acidity ratio (36.88) and reducing sugar content (8.28 g 100g-1). Blackberry fruits are a rich source of bioactive compounds, such as cv. Chickasaw obtaining a higher content of total phenolic compounds (1368.84 mg EAG100mL-1) and the Boysenberry hybrid a greater amount of anthocyanin (5.11mg Ci-3-Gly g -1). The Chickasaw cultivar has a higher lipid content (4.59). There is no difference in moisture content, dry biomass and fruit firmness.

Resumo A presente pesquisa teve como objetivo identificar os impactos na saúde mental e no cotidiano dos estudantes do curso de graduação em terapia ocupacional de uma universidade pública federal frente ao cenário de enfrentamento da COVID-19. Trata-se de um estudo exploratório e descritivo, de abordagem quantiqualitativa. Para coleta de dados, utilizou-se um questionário semiestruturado organizado no Google Formulários®. Participaram do estudo 53 graduandos do primeiro ao último ano do curso. Os resultados apontam que 94% dos participantes são do gênero feminino, com idade entre 20 e 25 anos (78%). Antes da pandemia, os estudantes realizavam uma vasta gama de atividades semanais, sendo que 68% não apresentavam questões prévias de saúde mental ou não apresentavam dificuldades que impactavam a sua vida de forma significativa. Com o advento da pandemia e suas consequências, o estudo apontou que 94% dos participantes observaram sua saúde mental impactada e, desses, 92% sinalizaram a ansiedade como a manifestação mais recorrente. Verificou-se ainda que praticamente todas as atividades que compunham o cotidiano dos estudantes antes da pandemia da COVID-19 foram alteradas de alguma maneira, sendo a categoria socialização a segunda mais citada. Diante do cenário atual de permanência da pandemia há mais de um ano, importa investigar como e quais estratégias de enfrentamento os estudantes, mas também a população geral, têm adotado para lidar com os persistentes e permanentes desafios. Assim, aponta-se para a necessidade e importância de se investir em pesquisas que aprofundem a compreensão desses impactos e das possíveis estratégias de enfrentamento adotadas.

Abstract This research aimed to identify the impacts on mental health and the daily lives of students of the undergraduate course in Occupational Therapy at a Federal Public University in the face of the COVID-19 coping scenario. This is an exploratory and descriptive study, with a quantitative and qualitative approach. For data collection, a semi-structured questionnaire organized in Google Forms was used. Fifty-three undergraduate students from the first to the last year of the course participated in the study. The results show that 94% of the participants are female, aged between 20 and 25 years (78%). Before the pandemic, students performed a wide range of weekly activities, 68% of whom had no previous mental health issues, or had no difficulties that significantly impacted their lives. With the advent of the pandemic and its consequences, the study found that 94% of participants observed their mental health impacted, and of these, 92% showed anxiety as the most recurrent manifestation. It was also found that practically all activities that made up students' daily lives before the COVID-19 pandemic were altered in some way, with the socialization category being the second most cited. Given the current scenario, in which the pandemic has been in existence for more than a year, it is important to investigate how and which coping strategies not only students but also the general population, have adopted to deal with the persistent and permanent challenges. Thus, it points to the need and importance of investing in research that deepens the understanding of these impacts and the possible coping strategies adopted.

RESUMO Objetivo: Avaliar o conhecimento dos profissionais da ortodontia quanto à prescrição de dentifrícios e antissépticos durante o tratamento ortodôntico. Métodos: Foi realizado um estudo observacional e descritivo exploratório de abrangência nacional com 440 ortodontistas brasileiros, baseado em cálculo amostral prévio, para avaliação da prática clínica dos ortodontistas na prescrição de dentifrícios e antissépticos. Questionários eletrônicos auto administrados foram enviados aos profissionais da Ortodontia. Os dados foram analisados por meio de tabelas de distribuição de frequência absolutas e relativas. Resultados: Verificou-se que o antisséptico bucal mais indicado (39,8%) foi o Colgate Periogard® (Colgate®) e 421 dos participantes (95,7%) conhecem o seu ingrediente ativo (digluconato de clorexidina). Além do antisséptico Colgate Periogard®, os participantes também foram questionados sobre qual o ingrediente ativo presente nos seguintes antissépticos, cujas respostas assertivas estavam na seguinte ordem %, Cepacol® (Sanofi-Aventis®) com 60,7%, Colgate Plax® (Colgate®) com 54,5% e Listerine® (Jhonson&Jhonson®) com 51,6%. Quanto ao regime terapêutico da clorexidina 63,9% indicaram assertivamente sua utilização sendo a mesma de 2 vezes ao dia durante 7 a 10 dias. O dentifrício mais indicado foi o Colgate Total 12®, sendo que 60,18% desses indivíduos indicaram corretamente seu composto ativo. Conclusão: De acordo com a metodologia empregada, pôde-se concluir que os profissionais da ortodontia conhecem e prescrevem métodos de controle químico aos pacientes durante o tratamento ortodôntico e a maioria identifica os compostos químicos ativos presentes nas fórmulas de antissépticos e dentifrícios.

ABSTRACT Objective: To evaluate the knowledge of orthodontic professionals regarding the prescription of dentifrices and antiseptics during orthodontic treatment. Methods: An observational and descriptive exploratory study of national scope was conducted with 440 Brazilian orthodontists, based on previous sample calculations, to evaluate the clinical practice of orthodontists in prescribing dentifrices and antiseptics. Self-managed electronic questionnaires were sent to orthodontic professionals. The data were analyzed by means of absolute and relative frequency distribution tables. Results: It was verified that the most indicated mouthwash (39.8%) was Colgate Periogard® and 421 of the participants (95.7%) know its active ingredient (chlorhexidine digluconate). Besides Colgate Periogard® antiseptic, the participants were also questioned about which active ingredient present on the following antiseptics, whose assertive answers were in the following order of %, Cepacol® (Sanofi-Aventis®) with 60.7%, Colgate Plax® (Colgate®) with 54.5% and Listerine® (Jhonson&Jhonson®) with 51.6%. As for the chlorhexidine therapeutic regime, 63.9% have stated assertively its use, being this one of 2 times a day during 7 to 10 days. The most indicated tooth was Colgate Total 12®, and 60.18% of these individuals correctly indicated its active compound. Conclusion: According to the methodology employed, it could be concluded that orthodontic professionals know and prescribe methods of chemical control to patients during orthodontic treatment and most identify the active chemical compounds present in antiseptic and tooth formulas.

ABSTRACT The objective of this work was to evaluate asepsis protocols, composition and concentration of culture media in the in vitro establishment of three physalis species. In experiment I, seeds of 3 species were used x 3 asepsis protocols. In experiment II, 3 culture media and explants 3 species were used. In experiment III, explants of 2 species x 4 concentrations of MS culture medium were used. In experiment IV, explants of 2 species x 4 sucrose concentrations were used. For experiments II, III and IV after 30 days were evaluated some phytotechnical parameters. The experimental design was completely randomized, in a 3 x 3 (experiments I and II) and 2 x 4 (experiments III and IV) factorial scheme. Protocols II and III were appropriate for P. peruviana germination, and I and III for P. minima, the three protocols were efficient for controlling fungi and bacteria. The MS is the most suitable for the in vitro establishment of three species. The culture medium at 100% concentration obtained better values in the in vitro development parameters evaluated for the species P. peruviana and P. minima. Sucrose concentrations close to 50 and 20 g L-1 favored the establishment of P. peruviana and P. minima.

ABSTRACT The objective of this study was to evaluate the potential of rooting of podocarpos cuttings in function of the number of leaves, concentrations of IBA and phytorregulators, application methods and cutting season. Two experiments were carried out under weaning conditions, in a factorial scheme containing 3 replicates of 15 cuttings, the first with 4 concentrations of IBA, 3 leaves at the cuttings and 2 seasons. These were removed in August/2016 and January/2017, prepared with 10 cm in length and immersed in solutions for 10 s. In the second experiment three types of phytoregulators and 2 application forms were tested (slow and quick immersion). Cuttings were prepared in the same manner as in the first experiment, maintaining 4 leaves. The bases of the cuttings were immersed for 10 s and 24 h in the solutions, then taken to root. After 100 days of experimentation, it was concluded that cuttings collected in the summer had a higher percentage of rooting, 4 leaves and IBA concentration should be maintained between 750 and 800 mg L-1. The best form of application of phytoregulator is by rapid immersion.

Resumo Como a região oeste do Paraná é considerada subtropical, com altas temperaturas no verão, os híbridos de Encyclia são comercializados pela beleza de suas flores e durabilidade. No entanto, não existem estudos envolvendo plantas em vasos, tipos de substratos e fertilizantes, além dos híbridos para o gênero Encyclia. Diante do exposto, o objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e o florescimento de Encyclia com base na adubação e no substrato.O experimento foi conduzido em um produtor comercial, de janeiro a setembro de 2017. As mudas permaneceram em ambiente protegido com 50% de sombreamento. O delineamento experimental foi em blocos casualizados, em esquema fatorial 4 x 5 (substratos x intervalos de fertilização), com 4 repetições por tratamento e 4 vasos por repetição. O lote escolhido era oriundo de semeadura in vitro, com o híbrido Encyclia “Kropp” x E. atropurpurea. Após cinco meses da última fertilização, foram realizadas avaliações relacionadas ao desenvolvimento vegetativo e floração. Os substratos à base de esfagno apresentaram melhores resultados no florescimento. As adubações orgânicas com Bokashi aos 45 dias após o transplante (DAT) favorecem a floração e o desenvolvimento vegetativo do híbrido de Encyclia, ocorrendo em vasos contendo substratos à base de esfagno e com adubações orgânicas acima de 60 DAT.

Abstract Due to the fact that the western region of Paraná is considered subtropical, with high temperatures in the summer, the hybrids of Encyclia are commercialized by the beauty of its flowers and durability. However, there are no studies involving potted plants, substrate types and fertilizers, as well as hybrids, for the genus Encyclia. Thus, the objective of this work was to evaluate the vegetative development and flowering of Encyclia based on fertilization and substrate. The experiment was conducted in a commercial orchard, from January to September/2017. The seedlings remained in a protected environment, with 50% shading. The experimental design was randomized blocks, in a 4 x 5 factorial scheme (substrates x fertilization intervals), with 4 replicates per treatment, and 4 pots per replicate. The lot chosen was sowed in vitro, with the hybrid Encyclia “Kropp” x E. atropurpurea. After five months of the last fertilization, the evaluations related to vegetative development and flowering were carried out. Substrates based on sphagnum presented better results in flowering. Organic fertilizations with Bokashi at 45 days after transplant (DAT) favor flowering and vegetative development of the Encyclia hybrid, which occurs in pots containing sphagnum-based substrates and with organic fertilizers above 60 DAT.