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Valorization of passion fruit: development, characterization and optimization of biodegradable edible films
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Macedo, Maria Clara Coutinho

; Silva, Viviane Dias Medeiros

; Rodrigues, Camila Gonçalves

; Pereira, Débora Tamires Vitor

; Garcia, Maria Aparecida Vieira Teixeira

; Pires, Christiano Vieira

; Fante, Camila Argenta

Polímeros

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2025, Volume 35 Nº 1 elocation e20250002

Resumo: En | Texto: En | PDF: En

Abstract This work aimed to develop biodegradable films from yellow passion fruit (Passiflora edulis f. flavicarpa) peel flour. The Central Composite Rotational Design was used in a total of 11 tests, in which the independent variables were the concentrations of starch and glycerol, characterized with regard to physical, mechanical and barrier properties. The films were classified as soluble and showed low permeability to water vapor. The tensile strength, elastic modulus and deformation rate were directly related to the concentration of starch and glycerol. The film of test 7 had the best characterization results; therefore, it was analyzed for biodegradability. As for biodegradability, the film obtained an average mass loss of 92.77 ± 4.28%, being a great alternative to the use of non-biodegradable polymers. The films showed acceptable degree of plasticization, which was favored by intermolecular interactions between the components of the flour, starch and glycerol.

https://doi.org/10.1590/0104-1428.20240043

Impact of Gamma Irradiation on Physicochemical, Technological, Antioxidant and Microbiology Properties of Whole Sorghum Flours Physicochemical Technological
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Correia, Vinícius T. V

; D’Angelis, Danielle F

; Queiroz, Valéria A. V

; Ramos, Ana Luiza C. C

; Macedo, Maria Clara C

; Augusti, Rodinei

; Melo, Júlio O. F

; Ferreira, Andreza A

; Lacerda, Inayara C. A

; Fante, Camila A

Journal of the Brazilian Chemical Society

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Fev 2023, Volume 34 Nº 2 Páginas 182 - 193

Resumo: En | Texto: En | PDF: En

This study aimed to evaluate the effect of gamma irradiation (GI) on the physicochemical, technological, antioxidant and microbiological characteristics of different whole sorghum flours (WSF), as well as to characterize the profile of chemical constituents by paper spray mass spectrometry (PS-MS). The doses applied interfered in the staining characteristics of the evaluated flours, providing the darkening of the same. For the other physicochemical, technological, and antioxidant parameters, no significant influence of the process was observed. The fingerprint obtained in both ionization modes had not been influenced by irradiation, being flavonoids, phenylpropanoids, amino acids, benzoic acid derivatives, carboxylic acids, and sugars tentatively identified. In microbiological terms, there was a reduction of molds, yeasts, and Bacillus cereus in irradiated WSF compared to control (non-irradiated). Therefore, the application of low doses of gamma irradiation represents an advantageous alternative for the conservation of WSF and maintenance of bioactive compounds identified by the PS-MS technique. GI (GI physicochemical technological WSF, , (WSF) PSMS. PSMS PS MS . (PS-MS) same parameters observed flavonoids phenylpropanoids acids derivatives terms molds yeasts nonirradiated. nonirradiated non (non-irradiated) Therefore technique (WSF (PS-MS (non-irradiated

https://doi.org/10.21577/0103-5053.20220097

Characterization and identification of bioactive compounds in agro-food waste flours
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Rodrigues, Camila Gonçalves

; Silva, Viviane Dias Medeiros Loyola, Anna Cláudia de Freitas e Silva, Mauro Ramalho Augusti, Rodinei Melo, Julio Onesio Ferreira Carlos, Lanamar de Almeida Fante, Camila Argenta

Química Nova

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2022, Volume 45 Nº 4 Páginas 403 - 409

Resumo: En | Texto: En | PDF: En

One of the recurrent problems of the agroindustry sector is the incorrect disposal of waste because of the complexity of the material, moisture, among other factors. Waste is not used in tonnage, but is systematically used. Examples of residues are malt bagasse and cassava peel, produced in large quantities in the brewing industry and flour production, respectively. In this context, the objective of this study was to evaluate bioactive compounds of flours produced from agroindustry waste to potentiate their use as an ingredient rich in antioxidants. The flours produced from agroindustry waste were evaluated for phenolic compounds, total carotenoids, antioxidant activity, and the attainment of phenolic profiles and fingerprints by paper spray mass spectrometry. It was possible to identify a broad class of compounds in both flours, such as phenolic acids, flavonoids, among others. The flours can be possibly included in food commodities or packaging formulation since they are natural antioxidants, thus adding value to these products and reducing environmental issues. However, further analysis is needed to ensure bioavailability as well as food safety.

https://doi.org/10.21577/0100-4042.20170853

Use of Paper Spray Mass Spectrometry for Determining the Chemical Profile of Green Cavendish Banana (Musa AAA) Peel and Pulp Flours and Evaluation of Its Functional Potential
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Loyola, Anna Cláudia F. e

; Silva, Viviane D. M.

; Silva, Mauro R.

; Rodrigues, Camila G.

; Santos, Amanda N. dos

; Melo, Júlio O. F. Augusti, Rodinei Fante, Camila A.

Journal of the Brazilian Chemical Society

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Mai 2021, Volume 32 Nº 5 Páginas 953 - 963

Resumo: En | Texto: En | PDF: En

Green Cavendish banana peel and pulp flours were obtained by three drying methods: oven dryer at 70 °C; air fryer at 180 °C and domestic oven at 180 °C, being the latter two new possibilities. Bioactive constituents using paper spray ionization mass spectrometry (PS-MS), phenolic identification and quantification by ultra-performance liquid chromatography with UV-Visible detection (UPLC/UV-Vis) and antioxidant capacity were evaluated. Phenolic acids showed distinct thermal stability between the treatments. Gallic acid was the predominant compound, ranging from (29.56 to 1211.74 mg 100 g-1) and had higher concentration than that found in other bananas described in literature. Green Cavendish banana flour is an advantageous source of bioactive compounds and antioxidant capacity, especially its peel. 26 compounds were identified by PS-MS: phenolics, organic acids, sugars, amino acid, phytosterol, iridoid and coumarin derivatives. Green Cavendish banana flour has great functional potential, and the air fryer can be a promising alternative for drying.

https://doi.org/10.21577/0103-5053.20200243 177 downloads

Differences in children and adolescents with SARS-CoV-2 infection: a cohort study in a Brazilian tertiary referral hospital
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Marques, Heloisa Helena de Sousa

; Pereira, Maria Fernanda Badue

; Santos, Angélica Carreira dos

; Fink, Thais Toledo

; Paula, Camila Sanson Yoshino de

; Litvinov, Nadia

; Schvartsman, Claudio

; Delgado, Artur Figueiredo

; Gibelli, Maria Augusta Bento Cicaroni

; Carvalho, Werther Brunow de

; Odone Filho, Vicente

; Tannuri, Uenis

; Carneiro-Sampaio, Magda

; Grisi, Sandra

; Duarte, Alberto José da Silva

; Antonangelo, Leila

; Francisco, Rossana Pucineli Vieira

; Okay, Thelma Suely

; Batisttella, Linamara Rizzo

; Carvalho, Carlos Roberto Ribeiro de

; Brentani, Alexandra Valéria Maria

; Silva, Clovis Artur

; Eisencraft, Adriana Pasmanik Rossi Junior, Alfio Fante, Alice Lima Cora, Aline Pivetta Reis, Amelia Gorete A. de Costa Ferrer, Ana Paula Scoleze Andrade, Anarella Penha Meirelles de Watanabe, Andreia Gonçalves, Angelina Maria Freire Waetge, Aurora Rosaria Pagliara Silva, Camila Altenfelder Ceneviva, Carina Lazari, Carolina dos Santos Abellan, Deipara Monteiro Santos, Emilly Henrique dos Sabino, Ester Cerdeira Bianchini, Fabíola Roberta Marim Alcantara, Flávio Ferraz de Paes Ramos, Gabriel Frizzo Leal, Gabriela Nunes Rodriguez, Isadora Souza Pinho, João Renato Rebello Carneiro, Jorge David Avaizoglou Paz, Jose Albino Ferreira, Juliana Carvalho Ferranti, Juliana Ferreira Ferreira, Juliana de Oliveira Achili Framil, Juliana Valéria de Souza Silva, Katia Regina da Kanunfre, Kelly Aparecida Bastos, Karina Lucio de Medeiros Galleti, Karine Vusberg Cristofani, Lilian Maria Suzuki, Lisa Campos, Lucia Maria Arruda Perondi, Maria Beatriz de Moliterno Diniz, Maria de Fatima Rodrigues Fonseca, Maria Fernanda Mota Cordon, Mariana Nutti de Almeida Pissolato, Mariana Peres, Marina Silva Garanito, Marlene Pereira Imamura, Marta Dorna, Mayra de Barros Luglio, Michele Rocha, Mussya Cisotto Aikawa, Nadia Emi Degaspare, Natalia Viu Sakita, Neusa Keico Udsen, Nicole Lee Scudeller, Paula Gobi Gaiolla, Paula Vieira de Vincenzi Severini, Rafael da Silva Giannasi Rodrigues, Regina Maria Toma, Ricardo Katsuya Paula, Ricardo Iunis Citrangulo de Palmeira, Patricia Forsait, Silvana Farhat, Sylvia Costa Lima Sakano, Tânia Miyuki Shimoda Koch, Vera Hermina Kalika Cobello Junior, Vilson

Clinics

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2021, Volume 76 elocation e3488

Resumo: En | Texto: En | PDF: En

OBJECTIVES: To compare demographic/clinical/laboratory/treatments and outcomes among children and adolescents with laboratory-confirmed coronavirus disease 2019 (COVID-19). METHODS: This was a cross-sectional study that included patients diagnosed with pediatric COVID-19 (aged <18 years) between April 11, 2020 and April 22, 2021. During this period, 102/5,951 (1.7%) of all admissions occurred in neonates, children, and adolescents. Furthermore, 3,962 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection samples were processed in patients aged <18 years, and laboratory-confirmed COVID-19 occurred in 155 (4%) inpatients and outpatients. Six/155 pediatric patients were excluded from the study. Therefore, the final group included 149 children and adolescents (n=97 inpatients and 52 outpatients) with positive SARS-CoV-2 results. RESULTS: The frequencies of sore throat, anosmia, dysgeusia, headache, myalgia, nausea, lymphopenia, pre-existing chronic conditions, immunosuppressive conditions, and autoimmune diseases were significantly reduced in children and adolescents (p<0.05). Likewise, the frequencies of enoxaparin use (p=0.037), current immunosuppressant use (p=0.008), vasoactive agents (p=0.045), arterial hypotension (p<0.001), and shock (p=0.024) were significantly lower in children than in adolescents. Logistic regression analysis showed that adolescents with laboratory-confirmed COVID-19 had increased odds ratios (ORs) for sore throat (OR 13.054; 95% confidence interval [CI] 2.750-61.977; p=0.001), nausea (OR 8.875; 95% CI 1.660-47.446; p=0.011), and lymphopenia (OR 3.575; 95% CI 1.355-9.430; p=0.010), but also had less hospitalizations (OR 0.355; 95% CI 0.138-0.916; p=0.032). The additional logistic regression analysis on patients with preexisting chronic conditions (n=108) showed that death as an outcome was significantly associated with pediatric severe acute respiratory syndrome (SARS) (OR 22.300; 95% CI 2.341-212.421; p=0.007) and multisystem inflammatory syndrome in children (MIS-C) (OR 11.261; 95% CI 1.189-106. 581; p=0.035). CONCLUSIONS: Half of the laboratory-confirmed COVID-19 cases occurred in adolescents. Individuals belonging to this age group had an acute systemic involvement of SARS-CoV-2 infection. Pediatric SARS and MIS-C were the most important factors associated with the mortality rate in pediatric chronic conditions with COVID-19.

https://doi.org/10.6061/clinics/2021/e3488

USE OF PAPER SPRAY-MASS SPECTROMETRY TO DETERMINE THE CHEMICAL PROFILE OF RIPE BANANA PEEL FLOUR AND EVALUATION OF ITS PHYSICOCHEMICAL AND ANTIOXIDANT PROPERTIES
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Silva, Viviane D. M.

; Arquelau, Priscila B. F. Silva, Mauro R. Augusti, Rodinei Melo, Júlio O. F. Fante, Camila A.

Química Nova

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Mai 2020, Volume 43 Nº 5 Páginas 579 - 585

Resumo: En | Texto: En | PDF: En

In this work, the peels of ripe banana (Musa sp.), variety “Prata”, were used to prepare a flour, which was evaluated for its physicochemical characteristics and antioxidant activity. The characterization of its chemical profile was performed by a fast and simple method: paper spray-mass spectrometry. This flour had high carbohydrate content and total dietary fiber, and significant amount of total phenolic compounds with antioxidant activity. In addition, this flour exhibited higher water-holding, oil-holding and swelling capacities than those of fruit by-products reported in the literature. Fourier-transform infrared spectroscopy confirmed the presence of functional groups of cellulose, hemicellulose, and lignin, constituents of insoluble dietary fiber. By the paper spray-mass spectrometry technique, it was possible to suggest the presence of 26 substances such as organic acids, phenolic acids, flavonoids, sugars, quinones, and terpenoids. Thus, this study demonstrated that the ripe banana peel flour has potential application as an ingredient for functional foods.

https://doi.org/10.21577/0100-4042.20170521 744 downloads

Severe clinical spectrum with high mortality in pediatric patients with COVID-19 and multisystem inflammatory syndrome
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Pereira, Maria Fernanda Badue

; Litvinov, Nadia

; Farhat, Sylvia Costa Lima

; Eisencraft, Adriana Pasmanik

; Gibelli, Maria Augusta Bento Cicaroni

; Carvalho, Werther Brunow de Fernandes, Vinicius Rodrigues Fink, Thais de Toledo Framil, Juliana Valéria de Souza Galleti, Karine Vusberg Fante, Alice Lima Fonseca, Maria Fernanda Mota Watanabe, Andreia Paula, Camila Sanson Yoshino de Palandri, Giovanna Gavros Leal, Gabriela Nunes Diniz, Maria de Fatima Rodrigues Pinho, João Renato Rebello Silva, Clovis Artur Marques, Heloisa Helena de Sousa Rossi Junior, Alfio Delgado, Artur Figueiredo Andrade, Anarella Penha Meirelles de Schvartsman, Claudio Sabino, Ester Cerdeira Rocha, Mussya Cisotto Kanunfre, Kelly Aparecida Okay, Thelma Suely Carneiro-Sampaio, Magda Maria Sales Jorge, Patricia Palmeira Daenekas

Clinics

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2020, Volume 75 elocation e2209

Resumo: En | Texto: En | PDF: En

OBJECTIVES: To assess the outcomes of pediatric patients with laboratory-confirmed coronavirus disease (COVID-19) with or without multisystem inflammatory syndrome in children (MIS-C). METHODS: This cross-sectional study included 471 samples collected from 371 patients (age<18 years) suspected of having severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The study group comprised 66/371 (18%) laboratory-confirmed pediatric COVID-19 patients: 61 (92.5%) patients tested positive on real-time reverse transcription-polymerase chain reaction tests for SARS-CoV-2, and 5 (7.5%) patients tested positive on serological tests. MIS-C was diagnosed according to the criteria of the Center for Disease Control. RESULTS: MIS-C was diagnosed in 6/66 (9%) patients. The frequencies of diarrhea, vomiting, and/or abdominal pain (67% vs. 22%, p=0.034); pediatric SARS (67% vs. 13%, p=0.008); hypoxemia (83% vs. 23%, p=0.006); and arterial hypotension (50% vs. 3%, p=0.004) were significantly higher in patients with MIS-C than in those without MIS-C. The frequencies of C-reactive protein levels >50 mg/L (83% vs. 25%, p=0.008) and D-dimer levels >1000 ng/mL (100% vs. 40%, p=0.007) and the median D-dimer, troponin T, and ferritin levels (p<0.05) were significantly higher in patients with MIS-C. The frequencies of pediatric intensive care unit admission (100% vs. 60%, p=0.003), mechanical ventilation (83% vs. 7%, p<0.001), vasoactive agent use (83% vs. 3%, p<0.001), shock (83% vs. 5%, p<0.001), cardiac abnormalities (100% vs. 2%, p<0.001), and death (67% vs. 3%, p<0.001) were also significantly higher in patients with MIS-C. Similarly, the frequencies of oxygen therapy (100% vs. 33%, p=0.003), intravenous immunoglobulin therapy (67% vs. 2%, p<0.001), aspirin therapy (50% vs. 0%, p<0.001), and current acute renal replacement therapy (50% vs. 2%, p=0.002) were also significantly higher in patients with MIS-C. Logistic regression analysis showed that the presence of MIS-C was significantly associated with gastrointestinal manifestations [odds ratio (OR)=10.98; 95%CI (95% confidence interval)=1.20-100.86; p=0.034] and hypoxemia [OR=16.85; 95%CI=1.34-211.80; p=0.029]. Further univariate analysis showed a positive association between MIS-C and death [OR=58.00; 95%CI=6.39-526.79; p<0.0001]. CONCLUSIONS: Pediatric patients with laboratory-confirmed COVID-19 with MIS-C had a severe clinical spectrum with a high mortality rate. Our study emphasizes the importance of investigating MIS-C in pediatric patients with COVID-19 presenting with gastrointestinal involvement and hypoxemia.

https://doi.org/10.6061/clinics/2020/e2209 2260 downloads

ANTIOXIDANT ACTIVITY DURING STORAGE OF APPLES SUBJECTED TO IRRADIATION
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Fante, Camila Argenta Elias, Heloísa Helena de Siqueira Henrique, Paôla de Castro Boas, Ana Carolina Vilas Lima, Luiz Carlos de Oliveira

Ciência e Agrotecnologia

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Jun 2015, Volume 39 Nº 3 Páginas 269 - 275

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

<p>Nas últimas décadas, diversos estudos têm demonstrado o interesse pela conservação de frutas e, também, pelos benefícios do consumo de alimentos ricos em compostos com atividade antioxidante. O presente estudo foi realizado com o objetivo de quantificar os compostos e a atividade antioxidante e analisar fatores físico-químicos, durante o armazenamento pós-colheita de maçãs da cultivar Eva submetidas à irradiação gama nas doses de 0; 0,5; 1,0 e 1,5 kGy. A atividade antioxidante foi medida pelos métodos de 2,2-difenil-1-picril-hidrazila (DPPH) e de β-caroteno/ácido linolenico. Foram avaliados os teores de compostos fenólicos totais, ácido ascórbico, cor e teor de sólidos solúveis. Os resultados mostraram que, independentemente da dose de irradiação, houve um aumento em % SRL, durante o armazenamento pós-colheita das maçãs de Eva. Baixas doses de irradiação foram capazes de preservar os compostos fenólicos, manter os níveis de ácido ascórbico e evitar um aumento no teor de sólidos solúveis.</p>

<p>Nowadays, the number of studies about fruit conservation as well as the benefits of consumption of foods rich in antioxidant compounds was increased. This study aimed to quantify antioxidant compounds and their activity and to evaluate the physical and chemical changes during the post-harvest storage of Eva's apple cultivars that were subjected to gamma irradiation at doses of 0; 0.5; 1.0 and 1.5 kGy. The antioxidant activity was measured by the 2.2-diphenyl-1-picrylhydrazyl (DPPH) and the β-carotene/linolenic acid methods. Levels of total phenolics, ascorbic acid, color, and soluble solids were evaluated. The results showed that, regardless of the irradiation dose, there was an increase in % SRL during post-harvest storage of Eva's apples. Low irradiation doses were able to preserve the phenolic compounds, maintain the ascorbic acid levels and avoid an increase in the soluble solids content.</p>

https://doi.org/10.1590/S1413-70542015000300008 2850 downloads

Modified atmosphere efficiency in the quality maintenance of Eva apples
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Fante, Camila Argenta Boas, Ana Carolina Vilas Paiva, Vivian Aguiar Pires, Caroline Roberta Freitas Lima, Luiz Carlos de Oliveira

Food Science and Technology

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Jun 2014, Volume 34 Nº 2 Páginas 309 - 314

Resumo: En | Texto: En | PDF: En

Modified atmosphere is a method of food preservation that provides increased lifetime, decreases deterioration losses, and facilitates marketing. The objective of this study was to evaluate the efficiency of different plastic films in modifying the atmosphere around Eva apples to assure quality maintenance during postharvest storage. The fruits were cleaned and separated into three treatment groups: polypropylene, low density polyethylene, and high density polyethylene packing with a total of 5 fruits per package for each evaluation period. A group of control apples was not submitted to atmospheric modification. After the treatment, all fruits were stored at 0.5±0.5°C (cold storage) for up to 225 days. The analyses were performed at 45, 135, and 225 days after cold storage. Respiration, ethylene production, firmness, mass loss, total pectin, soluble pectin, soluble solids, total acidity, and epidermis background color of each treatment group were evaluated. The high density polyethylene film treatment did not show a decrease in ethylene production during storage and allowed the fruits to maintain a greater firmness and smaller percentage of mass loss during the study period. Moreover, the storage of the Eva apple cultivar under modified atmosphere allowed the preservation of quality for up to seven months.

https://doi.org/10.1590/fst.2014.0044 4148 downloads Citado 1 vez em SciELO

10.

Effect of postharvest temperature on the shelf life of gabiroba fruit (Campomanesia pubescens)
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Silva, Edson Pablo da Cardoso, Andre Feres Lopes Fante, Camila Rosell, Cristina Molina Vilas Boas, Eduardo Valério de Barros

Food Science and Technology

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Dez 2013, Volume 33 Nº 4 Páginas 632 - 637

Resumo: En | Texto: En | PDF: En

The objective of this study was to determine the optimal temperature for storing gabiroba fruit (Campomanesia pubescens) without affecting compounds' quality. The fruits were stored at different temperatures (0 ºC, 6 ºC, 12 ºC, and 20 ºC) and the effect on the pH, total titratable acidity, soluble solids, total sugars, vitamin C, and antioxidant components such as tannins and total phenolic compounds was evaluated. It was observed an increase in the pH and total titratable acidity during storage at all the temperatures tested. Gabiroba fruits were stored for 9 and 3 days at 12 ºC and 20 ºC, respectively, and under both temperatures they showed a reduction in tannins and an increase in vitamin C content. As gabirobas armazenadas a 0º and 6 ºC alcançaram maior tempo de armazenamento After 12 days of storage, the fruits stored at 6 ºC contained higher amounts of water soluble solids, sugars, and antioxidants. In general, for long term storage, it is suggested to store gabiroba fruits at 6 ºC. On the other hand, for short term storage, the temperature of 12 ºC would be the better to keep high levels of vitamin C and phenolic compounds.

5750 downloads

11.

Pesquisadores testam a ação do 1-MCP em cultivar de maçãs Eva
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Fante, Camila

Ciência Rural

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Dez 2013, Volume 43 Nº 12

Resumo: Pt | Texto: Pt | PDF: Pt

330 downloads

12.

1-MCP nos aspectos fisiológicos e na qualidade pós-colheita de maçãs Eva durante o armazenamento refrigerado
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Fante, Camila Argenta Boas, Ana Carolina Vilas Costa, Adriano Carvalho Silva, Edson Pablo Oliveira, Marcelo Caetano de Lima, Luiz Carlos de Oliveira

Ciência Rural

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Dez 2013, Volume 43 Nº 12 Páginas 2142 - 2147

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O objetivo deste estudo foi verificar o efeito de doses específicas do composto 1-metilciclopropeno (1-MCP), após a colheita, nos aspectos fisiológicos e na qualidade físico-química de maçãs Eva durante o armazenamento refrigerado. Após a aplicação dos tratamentos com diferentes doses de 1-MCP (0, 10, 50, 100 e 500mg L-1), os frutos foram armazenados em câmara fria 0,5°C (±0,5) por até 135 dias. O armazenamento refrigerado juntamente com a aplicação de 1-MCP resultou em uma menor produção de etileno, principalmente nos frutos tratados com as doses de 10, 50 e 100mg L-1. O grupo controle perdeu 45% de sua firmeza, enquanto os grupos tratados perderam, em média, 27% de sua firmeza. O parâmetro C de cor identificou frutos com cor mais intensa aos 135 dias de armazenamento e o ângulo Hue mostrou que, quando tratados com as doses de 10 e 50mg L-1, os frutos apresentaram-se mais amarelados, evidenciando melhor ação do 1-MCP em baixas concentrações. Frutos que não foram submetidos aos tratamentos apresentaram maior relação sólidos solúveis e acidez, demonstrando assim maior atividade metabólica.

The objective of this study was to investigate the effect of specific doses of the compound 1-methylcyclopropene (1-MCP), after harvest, on the physiological and physicochemical quality of apples Eva during refrigerated storage. After applying thetreatment with different doses of 1-MCP (0, 10, 50, 100 and 500mg L-1), fruit were stored in cold 0.5°C (±0.5) for up to 135 days. The cold storage with the application of 1-MCP resulted in a lower production of ethylene in fruits treated with doses of 10, 50 and 100mg L-1. The control group lost 45% of its firmness, while the treated groups lost on average 27% of their firmness the parameter C fruit color identified with a more intense color to the 135 days of storage and Hue angle showed that when treated with doses of 10 and 50mg L-1, fruit showed more yellow, evidenced best action of 1-MCP at low concentrations. Fruit that have not undergone the treatments showed a higher ratio of soluble solids and acidity, thus demonstrating increased metabolic activity.

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13.

Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
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Fante, Camila Argenta Goulart, Patrícia de Fátima Pereira Alves, José Donizeti Henrique, Paôla de Castro Fries, Daniela Deitos

Ciência Rural

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Dez 2011, Volume 41 Nº 12 Páginas 2224 - 2229

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O estresse imposto às plantas pelo alagamento do solo constitui uma barreira para o crescimento e produtividade destas. A identificação de cultivares de soja que possam produzir maior teor de isoflavonas torna-se necessária à medida que a soja vem sendo utilizada na alimentação humana e para redução dos riscos de doenças crônicas. Dessa forma, o presente estudo foi realizado com o objetivo de quantificar isoflavonas e proteínas em cultivares de soja submetidas ao alagamento em diferentes estádios de desenvolvimento. As cultivares 'BRS 267', 'BRS 257' e 'BRS 213' foram submetidas a 15 dias de alagamento, nos estádios V6 e V8 e 11 dias sob estresse, no estádio R4. As proteínas no grão foram extraídas e quantificadas e analisadas as eletroforeses em SDS-PAGE. Os isoflavonóides foram extraídos, separados e quantificados por HPLC. A análise por eletroforese das três cultivares revelou o mesmo padrão de bandas relativas às proteínas totais, independente do tratamento utilizado. O alagamento promoveu aumento no teor total de isoflavonas nas plantas alagadas no estádio R4 da cultivar 'BRS 267', permanecendo constante nas demais cultivares.

The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.

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14.

Seleção de ameixeiras promissoras para a Serra da Mantiqueira
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Oliveira, Marcelo Caetano de Pio, Rafael Ramos, José Darlan Alvarenga, Ângelo Albérico Santos, Verônica Andrade dos Fante, Camila

Revista Ceres

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Ago 2011, Volume 58 Nº 4 Páginas 531 - 535

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

A ameixeira é espécie frutífera, cultivada comercialmente em regiões de inverno ameno a rigoroso, o que torna necessária a seleção de cultivares adequados quanto à adaptação climática. Este trabalho foi conduzido com o objetivo de selecionar materiais genéticos superiores, adaptados às condições edafoclimáticas da região sul de Minas Gerais, nas terras altas da Serra da Mantiqueira. As avaliações foram realizadas em cultivares de ameixeiras da espécie japonesa (Prunus salicina Lindl), 'Pretinha', 'Irati', 'Gema de Ouro', 'Reubennel', 'Roxa de Delfim Moreira', 'Gulfblaze', 'Frontier', 'Santa Rosa', e nove seleções (seleção I, seleção II, seleção III, seleção IV, seleção V, seleção VI, seleção VII, seleção VIII e seleção IX), enxertadas sobre o porta-enxerto 'Okinawa'. O delineamento experimental utilizado foi em blocos casualizados, com oito cultivares e nove seleções de ameixeira, com quatro repetições. Avaliou-se o desenvolvimento vegetativo das plantas, aos 18 e 24 meses após o plantio, no ciclo produtivo 2007/2008, além dos dados fenológico de brotação, florescimento, frutificação e colheita. Conclui-se que as seleções III e IX apresentaram-se como precoces, as seleções VII, VIII, 'Gema de Ouro' e 'Reubennel' apresentaram-se como de meia-estação e os cultivares 'Irati' e 'Gulfblaze' como tardios. Os cultivares Irati, Gema de Ouro e Reubennel demonstraram adaptação às condições edafoclimáticas da região das terras altas da Serra da Mantiqueira de Minas Gerais.

salicina Lindl) is a fruit species commercially cultivated in regions of mild and severe winters, and cultivar selection is mainly based on climate adaptation. The objective of this study was to select superior genetic materials, adapted to edafoclimatic conditions to the south region of Minas Gerais State, in the highlands of Serra da Mantiqueira. The evaluations were carried out with the plum cultivars 'Pretinha', 'Irati', 'Gema de Ouro', 'Reubennel', 'Roxa de Delfim Moreira', 'Gulfblaze', 'Frontier', 'Santa Rosa', and nine selections (selection I, selection II, selection III, selection IV, selection V, selection VI, selection VII, selection VIII and selection IX), grafted onto the rootstock 'Okinawa'. The experiment was arranged in arandomized block design, with eigth cultivars and nine selections, with four repetitions. Plant vegetative development of eighteen, and twenty-four month old plum trees was evaluated during the crop cycle 2007/2008, besides phenological data on sprouting, blooming, fruiting and harvest. The results showed that the selections III and IX were earlier; selections VII, VIII, 'Gema de Ouro' and 'Reubennel' were mid-season; and 'Irati' and 'Gulfblaze' were late. Cultivars Irati, Gema de Ouro and Reubennel showed better adaptation to soil and climatic conditions to the region of the highlands of the Mantiqueira Mountain Range in Minas Gerais.

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15.

Respostas fisiológicas em cultivares de soja submetidas ao alagamento em diferentes estádios
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Fante, Camila Argenta Alves, José Donizeti Goulart, Patrícia de Fátima Pereira Deuner, Sidnei Silveira, Neidiquele Maria

Bragantia

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2010, Volume 69 Nº 2 Páginas 253 - 261

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

A soja é um dos produtos agrícolas de maior importância no Brasil, e segundo maior produtor mundial. A tolerância ao excesso de umidade do solo e da rápida recuperação com o término do alagamento são características desta cultura, porém, a deficiência de oxigênio, causada pelo excesso de água, é um dos fatores determinantes que pode reduzir sua produtividade. O objetivo deste trabalho foi verificar o comportamento de três cultivares de soja ao alagamento em diferentes estádios de desenvolvimento, por meio de análise da produção de massa seca, metabolismo de carboidratos e atividade das enzimas do metabolismo anaeróbio. O experimento foi desenvolvido na Universidade Federal de Lavras (MG), em 2007. As cultivares BRS 267, BRS 257 e BRS 213 foram submetidas a 15 dias de alagamento, nos estádios V6 e V8 e 11 dias no estádio R4. Os resultados revelaram redução na produção de massa seca nos tratamentos alagados no estádio V8 para a cultivar BRS 267 e no estádio V6 para BRS 257 e BRS 213; esta última foi a mais tolerante ao alagamento, pois obteve o menor decréscimo de massa seca em relação ao controle. O alagamento também induziu aumento nos teores de amido e açúcares solúveis totais nas folhas, assim como maior atividade das enzimas álcool desidrogenase e piruvato descarboxilase para as três cultivares, quando comparadas a seus respectivos controles. Já para a enzima lactato desidrogenase, essa diferença foi menos expressiva. Esses resultados revelam comportamentos distintos comparando as três cultivares.

The soybean is one of the most important agricultural products in Brazil, the second in world production. The soil high tolerance to excess of moisture and the rapid recovery after flooding are important characteristics of this culture. However, the oxygen, deficiency resulting from excess of water is one factor that may cause reduction in soybean productivity. This study aimed to evaluate the behavior of three soybeans cultivars upon flooding at various stages of development, based on dry matter production, carbohydrate metabolism and some anaerobic enzymes activities. The experiment was conducted at the Federal University of Lavras, Brazil, in 2007. Cultivars BRS 267, BRS 257 and BRS 213 were submitted to 15 days of flooding, for stages V6 and V8 and 11 days for stage R4. Results indicated a reduction in the production of dry matter for the treatments flooded BRS 267 in the stage V8 and for varieties BRS 257 and BRS 213, V6 stage the latter being the most tolerant to flooding, once exhibited a lower reduction in dry matter compaired to control. The flooding also lead to an increase of total soluble sugars and starch levels in the leaves, as well as to an increase in the activity of the enzymes alcohol dehydrogenase and pyruvate decarboxylase, in all three evaluated cultivars. Nom significant differences were observed for the lactate dehydrogenase enzyme activity. These results indicate a different behavior when comparing the three cultivars.

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