ABSTRACT Objective: This study aimed to compare the levels of HIF1-α, VEGF, TNF-α, and IL-10 in the peri-implant crevicular fluid of patients with and without peri-implantitis. Methods: Forty patients, comprising 16 with and 24 without peri-implantitis were selected. Results: Patients with peri-implantitis exhibited significantly higher HIF-1α levels than those without peri-implantitis (p=0.0005). TNF-α revealed significant positive correlations with IL-10 (p=0.0008) and VEGF (p=0.0246), whereas HIF-1α and IL-10 levels (p=0.0041) demonstrated a negative and significative correlation in the peri-implantitis group. Conclusion: This study, for the first time demonstrates the balance of HIF-1α, TNFα, IL-10, and VEGF in peri-implantitis. It shows an elevated HIF-1α levels in patients with peri-implantitis, which could have stemmed from persistent inflammation- triggered hypoxia. Furthermore, the positive correlation between TNF-α and VEGF suggests intensified proinflammatory activity in peri-implantitis. Nevertheless, further studies are essential to understand these immune dynamics in peri-implantitis. Objective HIF1α, HIF1α HIFα HIF1 α, α HIF HIF1-α TNFα TNF IL10 IL 10 IL-1 periimplant peri implant periimplantitis. periimplantitis implantitis. implantitis Methods 1 2 selected Results 1α p=0.0005. p00005 p p=0.0005 . 0 0005 (p=0.0005) p=0.0008 p00008 0008 (p=0.0008 p=0.0246, p00246 p=0.0246 , 0246 (p=0.0246) p=0.0041 p00041 0041 (p=0.0041 group Conclusion 1α, IL10, 10, periimplantitis, implantitis, inflammation hypoxia Furthermore Nevertheless IL1 IL- p0000 p=0.000 000 (p=0.0005 (p=0.000 p0024 p=0.024 024 (p=0.0246 p=0.004 p0004 004 (p=0.004 p000 p=0.00 00 (p=0.00 p002 p=0.02 02 (p=0.024 p00 p=0.0 (p=0.0 (p=0.02 p0 p=0. (p=0. p=0 (p=0 p= (p= (p

Abstract Isidore of Seville (c. 570-636) and Rodrigo Ximénez de Rada (1170-1247) mark the beginning and end point of a type of historiography in Iberia that is still very dependent on late antique models. Isidore’sChronicon(CPL 1205) andHistoriae(CPL 1204) were considered canonical models of what “writing history” should mean, forming the backbone of all major texts and compilations written in Iberia until the thirteenth century. In this paper, I analyze how Ximénez de Rada used Isidore’sHistoriaeat two levels: in structuring his own work and as a source. In terms of structure, I will show how theHistoriaewas the main model for Ximénez de Rada’s historiographical project. Concerning the use of Isidore’s text as a source, I will identify which versions of theHistoriaewere used by Ximénez de Rada, and analyze how, concretely, he adapted Isidore’s text. I will argue that Ximénez de Rada did not just copy theHistoriae, but took both Isidore’s structure and text, rethought them, and made a completely new work of his own.

Resumo Isidoro de Sevilha (c. 570-636) e Rodrigo Ximénez de Rada (1170-1247) marcam na Península Ibérica o início e o fim de um tipo de historiografia ainda muito dependente dos modelos da Antiguidade Tardia. O Chronicon (CPL 1205) e as Historiae (CPL 1204) de Isidoro foram consideradas como modelo canónico do que deveria significar “escrever história”, tornando-se a espinha dorsal de todos os grandes textos e compilações escritos na Península Ibérica até ao século XIII. Neste artigo, analiso a forma como Ximénez de Rada utilizou as Historiae de Isidoro em dois níveis diferentes: na estruturação de sua própria obra e como fonte. Em termos de estrutura, mostrarei como as Historiae foram de facto o principal modelo do projeto historiográfico de Ximénez de Rada. No que se refere ao uso do texto de Isidoro como fonte, identificarei quais as versões das Historiae que foram utilizadas por Ximénez de Rada e analisarei como, concretamente, o texto de Isidoro foi adaptado. Mostrarei também que Ximénez de Rada não apenas copiou, mas tomou a estrutura e o texto de Isidoro, repensando-os e construindo um texto completamente novo.

Objectives: To re-evaluate a sample of older adults enrolled in a randomized controlled trial of lithium for amnestic mild cognitive impairment (MCI) after 11 to 15 years, re-assessing their current (or last available) global cognitive and functional state. Methods: We recalled all former participants of the Lithium-MCI trial conducted by our group between 2009 and 2012 to perform a single-blinded, cross-sectional evaluation of their global clinical state to compare the long-term outcome of those who received lithium vs. those who received placebo. Results: Of the original sample (n=61), we were able to reach 36 participants (59% of retention), of whom 22 had previously received lithium (61% of the recall sample) and 14 (39%) had received placebo. Since 30.5% of the recalled sample was deceased, psychometric data were collected only for 69.5% of the participants. We found statistically significant differences in current mean Mini Mental State Examination score according to previous treatment group (25.5 [SD, 5.3] vs. 18.3 [SD, 10.9], p = 0.04). The lithium group also had better performance in the phonemic Verbal Fluency Test than the control group (34.4 [SD, 14.4] vs. 11.6 [SD, 10.10], p < 0.001). Differences in these measures also had large effect sizes, as shown by Cohen’s d values of 0.92 and 1.78, respectively. Conclusion: This data set suggests that older adults with amnestic MCI who had been treated with lithium during a previous randomized controlled trial had a better long-term global cognitive outcome than those from a matched sample who did not receive the intervention. Objectives reevaluate re evaluate (MCI 1 years reassessing assessing or available Methods LithiumMCI Lithium 200 201 singleblinded, singleblinded single blinded, blinded single-blinded crosssectional cross sectional longterm long term vs placebo Results n=61, n61 n n=61 , 61 (n=61) 3 59% 59 (59 retention, retention retention) 2 61% (61 39% 39 (39% 305 30 5 30.5 deceased 695 69 69.5 25.5 255 25 (25. SD, SD [SD 5.3 53 183 18 18. 10.9, 109 10.9 10 9 10.9] 0.04. 004 0.04 . 0 04 0.04) 34.4 344 34 4 (34. 14.4 144 116 6 11. 10.10, 1010 10.10 10.10] 0.001. 0001 0.001 001 0.001) sizes Cohens Cohen s 092 92 0.9 178 78 1.78 respectively Conclusion intervention 20 n6 n=6 (n=61 (5 (6 (39 30. 69. 25. (25 5. 10. 00 0.0 34. (34 14. 101 10.1 000 0.00 09 0. 17 7 1.7 n= (n=6 ( (3 (2 1. (n= (n

Abstract Background Cardiac arrest (CA) is a common condition associated with high mortality. The Brazilian advanced life support training TECA A (Treinamento em Emergências Cardiovasculares Avançado — Advanced Cardiovascular Emergency Training) was created to train healthcare professionals in the management of CA. However, there are no studies evaluating the effectiveness of TECA A. Objective To assess the impact of TECA A on the management of CA using a simulated CA situation. Methods Fifty-six students underwent a simulated case of CA in a manikin. The students’ performance in the management of CA was assessed for the time to first chest compression and defibrillation and for a global assessment score using a structured tool. These items were assessed and compared before and after the TECA A. Exclusion criteria were previous participation in CA trainings and absence from class. Categorical variables were compared using the McNemar test and quantitative variables using the Wilcoxon test. All tests were two-tailed, and statistical significance was set at p < 0.05. Results Compared with before TECA A, median global assessment scores were higher after TECA A (pre-training: 4.0 points [2.0-5.0] vs. 10 points [9.0-10.0]; p<0.001), the time to start chest compressions was shorter (pre-training: 25 seconds [15-34] vs. 19 seconds [16.2-23.0]; p=0.002) and so was the time to defibrillation (pre-training: 82.5 seconds [65.0-108.0] vs. 48 seconds [39.0-53.0]; p<0.001). Conclusions The TECA A promoted a higher adherence to cardiopulmonary resuscitation (CPR) guidelines and a reduction in the time elapsed from CA to first chest compression and defibrillation. (CA mortality Treinamento Training However situation Fiftysix Fifty six manikin tool class twotailed, twotailed two tailed, tailed two-tailed 005 0 05 0.05 pretraining pre (pre-training 40 4 4. 2.05.0 2050 2.0 5.0 2 5 [2.0-5.0 vs 1 9.010.0 90100 9.0 10.0 9 [9.0-10.0] p<0.001, p0001 p<0.001 , 001 p<0.001) 1534 15 34 [15-34 16.223.0 162230 16.2 23.0 16 23 [16.2-23.0] p=0.002 p0002 002 825 82 82. 65.0108.0 6501080 65.0 108.0 65 108 [65.0-108.0 39.053.0 390530 39.0 53.0 39 53 [39.0-53.0] p<0.001. . CPR (CPR 00 0.0 2.05. 205 20 2. 50 5. [2.0-5. 010 9.010. 9010 90 9. 100 10. [9.0-10.0 p000 p<0.00 153 3 [15-3 223 16.223. 16223 162 16. 230 23. [16.2-23.0 p=0.00 8 0108 65.0108. 650108 650 65. 1080 108. 6 [65.0-108. 053 39.053. 39053 390 39. 530 53. [39.0-53.0 0. 2.05 [2.0-5 01 9.010 901 [9.0-10. p00 p<0.0 [15- 22 16.223 1622 [16.2-23. p=0.0 65.0108 65010 [65.0-108 39.053 3905 [39.0-53. [2.0- 9.01 [9.0-10 p0 p<0. [15 16.22 [16.2-23 p=0. 65.010 6501 [65.0-10 39.05 [39.0-53 [2.0 [9.0-1 p<0 [1 [16.2-2 p=0 65.01 [65.0-1 [39.0-5 [2. [9.0- p< [ [16.2- p= [65.0- [39.0- [2 [9.0 [16.2 [65.0 [39.0 [9. [16. [65. [39. [9 [16 [65 [39 [6 [3

ABSTRACT BACKGROUND: Complete surgical resection is the treatment of choice for patients with liver metastases, but in some patients, it is not possible to obtain a complete R0 resection. Moreover, the recurrence rate is up to 75% after three years. After the experience of the Oslo group with cadaveric liver transplant, some centers are starting their experience with liver transplant for colorectal liver metastasis. AIMS: To present our initial experience with living donor liver transplant for colorectal liver metastasis. METHODS: From 2019 to 2022, four liver transplants were performed in patients with colorectal liver metastases according to the Oslo criteria. RESULTS: Four patients underwent living donor liver transplants, male/female ratio was 3:1, mean age 52.5 (42–68 years). All patients were included in Oslo criteria for liver transplant. Two patients had already been submitted to liver resection. The decision for liver transplant occurred after discussion with a multidisciplinary team. Three patients recurred after the procedure and the patient number 3 died after chemotherapy. CONCLUSIONS: Living donor liver transplant is a viable treatment option for colorectal liver metastasis in Brazil, due to a shortage of donors. BACKGROUND R Moreover 75 years AIMS METHODS 201 2022 RESULTS malefemale male female 31, 31 1, 1 3:1 525 52 5 52. 42–68 4268 42 68 (42–6 . years) team chemotherapy CONCLUSIONS Brazil donors 7 20 202 3: 42–6 426 4 6 (42– 2 42– (42 (4 (

RESUMO RACIONAL: A ressecção cirúrgica completa é o tratamento de escolha para pacientes com metástases hepáticas, mas em alguns pacientes não é possível obter uma completa ressecção R0. Além disso, a taxa de recorrência é de até 75% após 3 anos. Após a experiência do grupo de Oslo com transplante hepático cadavérico, alguns centros estão iniciando sua experiência com transplante hepático para metástase hepática colorretal. OBJETIVOS: Apresentar a experiência inicial com transplante de fígado de doador vivo para metástase hepática colorretal. MÉTODOS: De 2019 a 2022, foram quatro transplantes hepáticos em pacientes com metástases hepáticas colorretais, de acordo com os critérios de Oslo. RESULTADOS: Quatro pacientes foram submetidos a transplante hepático de doador vivo, a relação homem/mulher de 3:1, a idade média foi de 52,5 (42-68 anos). Todos os pacientes foram incluídos nos critérios de Oslo para transplante de fígado. Dois pacientes já haviam sido submetidos à ressecção hepática. A decisão pelo transplante hepático ocorreu após discussão com equipe multidisciplinar. Três pacientes recidivaram após o procedimento e o paciente número 3 morreu após a quimioterapia. CONCLUSÕES: O transplante de fígado com doador vivo é uma opção viável de tratamento para metástase hepática colorretal no Brasil, devido à escassez de doadores. RACIONAL R0 R disso 75 anos cadavérico OBJETIVOS MÉTODOS 201 2022 colorretais RESULTADOS homemmulher homem mulher 31, 31 1, 1 3:1 525 52 5 52, 4268 42 68 (42-6 . anos) multidisciplinar quimioterapia CONCLUSÕES Brasil doadores 7 20 202 3: 426 4 6 (42- 2 (42 (4 (

ABSTRACT Objective: To identify the lingual foramina in a Brazilian population of 210 individuals through Cone-Beam Computed Tomography images, in order to guide the installation of dental implants in the region of the mental symphysis. Methods: After identifying the lingual foramina on a parasagittal section, four measurements were taken: distance to the alveolar ridge, distance to the mandibular lower border, distance to the limit of the vestibular cortical, and alveolar ridge inclination angle. The measurements were compared in relation to gender by the Mann-Whitney test. The association between gender and the number of foramina was done by the chi-square test. Pearson’s correlation analyzed the linear relationship between age and number of foramina. Results: Lingual foramina are present in 99.6% of the research participants. In the upper-inferior direction, a mean distance to the alveolar ridge of 21.4 mm was noted, while the mean distance to the lower mandibular border was 13.1 mm. In the bucco-lingual direction, a mean distance from the lingual foramina to the vestibular cortical of 15.2 mm is noted. The inclination angle of the alveolar ridge had a mean of 25.4º. Regarding gender, the test was not significant only for the distance to the vestibular cortical. Conclusion: The lingual foramina had a descending way in 100% of cases, being positioned mainly in the middle thirds (66.5%) and lower thirds (32.7%) of the mandibular ridge height. The use of dental implants up to 13 mm in length and up to 4 mm in diameter in the region of the symphysis mentualis is recommended. Objective 21 ConeBeam Cone Beam images Methods section taken MannWhitney Mann Whitney chisquare chi square Pearsons Pearson s Results 996 99 6 99.6 participants upperinferior upper inferior direction 214 21. noted 131 1 13. buccolingual bucco 152 15 2 15. 254º º 25 4º 25.4º Conclusion 100 cases 66.5% 665 66 5 (66.5% 32.7% 327 32 7 (32.7% height recommended 9 99. 10 66.5 (66.5 32.7 3 (32.7 66. (66. 32. (32. (66 (32 (6 (3 (

RESUMO Objetivos: Identificar as foraminas linguais em uma população brasileira de 210 indivíduos, por meio de imagens de tomografia computadorizada de feixe cônico, a fim de orientar a instalação de implantes dentários em região de sínfise mentual. Métodos: Após identificação das foraminas linguais em corte parasagital, foram realizadas quatro medidas: distância até à crista alveolar, distância até à borda inferior da mandíbula, distância até o limite da cortical vestibular e ângulo de inclinação do rebordo alveolar. As medidas foram comparadas em relação ao sexo pelo teste Mann-Whitney. A comparação entre os sexos e o número de foraminas foi feito pelo teste qui-quadrado. A correlação de Pearson analisou a relação linear entre idade e número de foraminas. Resultados: As foraminas linguais estão presentes em 99,6% dos indivíduos. No sentido súpero-inferior, nota-se uma distância média até à crista alveolar de 21,4 mm, enquanto a distância média até à borda inferior da mandíbula foi de 13,1 mm. No sentido vestíbulo-lingual, nota-se uma distância média da foramina lingual até a cortical vestibular de 15,2 mm. O ângulo de inclinação do rebordo alveolar apresentou uma média de 25,4º. Em relação ao sexo, o teste só não foi significativo para a distância até a cortical vestibular. Conclusão: As foraminas linguais apresentam um trajeto descendente em 100% dos casos, posicionando-se, principalmente, nos terços médios (66,5%) e terços inferiores (32,7%) da altura do rebordo mandibular. Recomenda-se o uso de implantes dentários de até 13 mm de comprimento e de até 4mm de diâmetro em região de sínfise mentual. Objetivos 21 indivíduos cônico mentual Métodos parasagital MannWhitney. MannWhitney Mann Whitney. Whitney Mann-Whitney quiquadrado. quiquadrado qui quadrado. quadrado qui-quadrado Resultados 996 99 6 99,6 súperoinferior, súperoinferior súpero inferior, súpero-inferior notase nota se 214 4 21, 131 1 13, vestíbulolingual, vestíbulolingual vestíbulo lingual, vestíbulo-lingual 152 15 2 15, 254º º 25 4º 25,4º Conclusão 100 casos posicionandose, posicionandose posicionando se, posicionando-se principalmente 66,5% 665 66 5 (66,5% 32,7% 327 32 7 (32,7% mandibular Recomendase Recomenda 9 99, 10 66,5 (66,5 32,7 3 (32,7 66, (66, 32, (32, (66 (32 (6 (3 (

Abstract We evaluated the genetic diversity, coancestry and heritability of an E. benthamii trial. The 115 individuals were genotyped (13 SSR) and had their height and diameter at breast height (dbh) measured. Heritability was estimated using the RR-BLUP and the pairwise kinship coefficient method. An average of nine alleles per locus was observed. The expected heterozygosity (0.655) was similar to the observed heterozygosity, with the estimated inbreeding (0.02) being low. The group coancestry (0.051) demonstrates that the trees are related to some degree. The trees were clustered in five groups using the Evanno’s method. The average kinship within each group ranges from 0.042 to 0.082. The heritability estimated by RR-BLUP was low. The heritability estimated using the kinship coefficients is moderate, reaching estimated genetic gains of 14% for dbh. After knowing how genetic groups are distributed within the population, strategies for collecting, conserving, and using these germplasm resources can be performed. diversity E trial 11 13 (1 SSR dbh (dbh measured RRBLUP RR BLUP method 0.655 0655 0 655 (0.655 0.02 002 02 (0.02 low 0.051 0051 051 (0.051 degree Evannos Evanno s 0042 042 0.04 0082 082 0.082 moderate 14 population collecting conserving performed 1 ( 0.65 065 65 (0.65 0.0 00 (0.0 0.05 005 05 (0.05 004 04 008 08 0.08 0.6 06 6 (0.6 0. (0. (0

ABSTRACT: The use of unmanned aerial vehicles (UAVs) in the agricultural and forestry sectors has constantly evolved due to its great versatility and applicability in the field. In this sense, this study provided a statistical overview of studies on the use of UAVs in agricultural and forestry through a bibliometric and scientometric analysis. For that, a research was carried out on the Scopus platform using the terms UAV, UAS, drone, and RPA, together with “agricult*” or “forest* or livestock”. Only manuscripts published in English and from January 2000 to December 2020 were selected. The VOSviewer software was used for the analyses. The USA and China were responsible for more than 38% of the publications worldwide. Furthermore, about 50% of the countries in the world showed some scientific record of the use of UAVs in agricultural and forestry studies. The term UAS was more used until 2016, while UAV was more mentioned between 2017 and 2018. Conversely, drone was more endorsed from 2019. The constant increase in scientific production reported in the research and the evolution of the co-occurrence of keywords corroborated two ideas: i) the use of UAVs is still undergoing transformations and is directly related to the advancement of technology included in these equipments; and ii) studies are still not enough to explore all the applicability of the UAVs in agriculture, livestock and forestry. ABSTRACT (UAVs field sense analysis that RPA agricult* agricult “agricult* forest* forest “forest livestock. . livestock” 200 202 selected analyses 38 worldwide Furthermore 50 2016 201 2018 Conversely 2019 cooccurrence co occurrence ideas i equipments ii agriculture “agricult 20 3 5 2

RESUMO: A utilização de veículos aéreos não tripulados (VANTs) nos setores agrícola e florestal tem evoluído constantemente devido a sua grande versatilidade e aplicabilidade no campo. Nesse sentido, este trabalho teve como objetivo fornecer um panorama estatístico dos estudos sobre o uso de VANTs na agropecuária e silvicultura por meio de uma análise bibliométrica e cientométrica. Para isso, foi realizada uma pesquisa na plataforma Scopus utilizando os termos UAV, UAS, drone e RPA, juntamente com “agricult*” ou “forest* ou livestock”. Foram selecionados apenas manuscritos publicados em inglês, de janeiro de 2000 a dezembro de 2020. O software VOSviewer foi utilizado para as análises. Os EUA e a China foram responsáveis por mais de 38% das publicações mundiais. Além disso, cerca de 50% dos países do mundo apresentaram algum registro científico do uso de VANTs em estudos agrícolas e florestais. O termo UAS foi mais utilizado até 2016, enquanto o UAV foi mais mencionado entre 2017 e 2018. Por outro lado, drone foi mais endossado a partir de 2019. O constante aumento da produção científica encontrado nas pesquisas e a evolução da coocorrência de palavras-chave corroboraram duas ideias: i) o uso de VANTs ainda está passando por transformações e está diretamente relacionado ao avanço da tecnologia inserida nesses equipamentos; e ii) os estudos ainda não são suficientes para explorar toda a aplicabilidade dos VANTs na agricultura, pecuária e silvicultura. RESUMO (VANTs campo sentido cientométrica isso RPA agricult* agricult “agricult* forest* forest “forest livestock. livestock . livestock” inglês 200 2020 análises 38 mundiais disso 50 florestais 2016 201 2018 lado 2019 palavraschave palavras chave ideias i equipamentos ii agricultura “agricult 20 202 3 5 2

Aim To evaluate the resistance of the union between a glass fiber post and radicular dentine after cleaning the root with 17% EDTA and filling with different endodontic cements. Methods Forty uniradicular bovine incisors were removed to obtain root lengths of 18 mm. Endodontic treatment was performed on all roots using different filling cements (zinc oxide and eugenol-based, OZE; cement based on epoxy resin, AH) and cleaning solutions (saline, SA or EDTA), which made it possible to obtain four groups: OZESA, OZEEDTA, AHSA and AHEDTA. Subsequently, 12 mm of filling material was removed from the roots, and they were prepared to receive fiber posts luted with resin cement. To execute the mechanical cycles (2x106 cycles, 90 N, 4 Hz), coronal reconstruction was performed with a silicon matrix. The roots were then sliced (2-mm thick) to perform the push-out test. The results were analyzed using analysis of variance (one factor and two factors) and Tukey’s test (α=0,05). Results Bond strength (Mpa) was significantly higher for OZEEDTA (9,18) and AHEDTA (8,70) than for OZESA (6,06) AHSA (8,7). OZEEDTA also presented the highest values in the cervical region (15,18) but was significantly lower in the apical region (2,99). However, AHEDTA had a homogeneous bond strength in all thirds. Conclusion Regardless of the endodontic cement used, EDTA was used as an irrigating solution, culminating in a higher bond strength between the glass fiber post and dentin.

Resumo Fundamento A consolidação de novos paradigmas educacionais exige a implantação de estratégias inovadoras com potencial de transformar estudantes em profissionais competentes. Objetivos Analisar o conhecimento e a satisfação de estudantes antes e após a utilização de uma nova metodologia ativa de ensino médico de modelo digital humanizado chamada Virtual Case-Based Learning (VCBL). Métodos Estudo descritivo com análise documental sobre o processo de ensino-aprendizagem de estudantes de medicina. Dados obtidos da avaliação de conhecimento teórico e do instrumento de satisfação dos alunos nos anos de 2018 e 2019 foram analisados, e a nova metodologia proposta VCBL foi comparada com a metodologia ativa de ensino tradicional, o Problem-Based Learning (PBL). As análises descritivas e de associação foram realizadas utilizando o programa Statistical Package for the Social Sciences. Resultados Foram analisados 167 documentos aplicados a estudantes de medicina. Em relação à avaliação do conhecimento teórico, os alunos avaliados em 2018 obtiveram média 41,7%, comparados aos alunos de 2019 que alcançaram 73,3% (p<0,001). Entre os estudantes submetidos à avaliação da satisfação com a metodologia de aprendizagem proposta, 76,0% pontuaram o valor máximo para a questão um, e 83,0% para a questão número dois. Cerca de 70,0% dos estudantes classificaram positivamente o aprendizado adquirido após utilização da plataforma Paciente 360; 78,0% responderam que se sentem preparados para o atendimento ambulatorial; e 94,0% pontuaram de forma positiva a metodologia empregada. Conclusão Neste estudo inicial, os resultados indicaram que a nova ferramenta em metodologia ativa de ensino médico digital humanizado, o VCBL, pode auxiliar no aprimoramento do processo de ensino-aprendizagem, proporcionando conhecimento e satisfação dos estudantes.

Abstract Background The consolidation of new educational paradigms requires the implementation of innovative strategies to transform students into competent professionals. Objectives To assess knowledge and satisfaction of medical students before and after the use of a new humanized digital model of active learning, called virtual case-based learning (VCBL). Methods This was a descriptive, documentary analysis of the teaching-learning process of medical students. Data obtained from theoretical knowledge assessment and satisfaction evaluation questionnaires applied in 2018 and 2019 were analyzed, and the new VCBL was compared with the traditional active methodology PBL (problem-based learning). Descriptive and association analyses were made using the Statistical Package for the Social Sciences. Results A total of 167 evaluation forms administered to medical students were analyzed. In the evaluation of theoretical knowledge, the 2018 and the 2019 student groups had a mean of 41.7% and 73.3%, respectively (p<0.001). Among the students who responded to the satisfaction evaluation form, 76.0% gave the highest rating to question one, and 83.0% to question two. Nearly 70.0% of students positively evaluated knowledge acquisition with the Paciente 360 platform; 78.0% reported to feel prepared for working in outpatient care; and 94.0% positively evaluated the new method. Conclusion In this initial study, the results indicate that the new active method for humanized digital medical education, the VCBL, can help in the betterment of the teaching-learning process, promoting knowledge and satisfaction by the students.

Abstract Cardiovascular diseases are the leading cause of death in the world. People living in vulnerable and poor places such as slums, rural areas and remote locations have difficulty in accessing medical care and diagnostic tests. In addition, given the COVID-19 pandemic, we are witnessing an increase in the use of telemedicine and non-invasive tools for monitoring vital signs. These questions motivate us to write this point of view and to describe some of the main innovations used for non-invasive screening of heart diseases. Smartphones are widely used by the population and are perfect tools for screening cardiovascular diseases. They are equipped with camera, flashlight, microphone, processor, and internet connection, which allow optical, electrical, and acoustic analysis of cardiovascular phenomena. Thus, when using signal processing and artificial intelligence approaches, smartphones may have predictive power for cardiovascular diseases. Here we present different smartphone approaches to analyze signals obtained from various methods including photoplethysmography, phonocardiograph, and electrocardiography to estimate heart rate, blood pressure, oxygen saturation (SpO2), heart murmurs and electrical conduction. Our objective is to present innovations in non-invasive diagnostics using the smartphone and to reflect on these trending approaches. These could help to improve health access and the screening of cardiovascular diseases for millions of people, particularly those living in needy areas.

Abstract This study investigated the depuration time of tilapia in saline and freshwater environments and its influence on animal performance and meat quality. Depuration until the seventh day resulted in significant reductions in average weight, condition factor, viscerosomatic index, hepatosomatic index and fillet yield in both environments. Regarding proximate composition, the saline environment promoted the maintenance of crude protein, moisture and ash percentages. Depuration in the freshwater environment resulted in reduced percentages of crude protein, ash and energy and increased humidity. Total lipids were reduced in both environments, but with a significantly lower in saline. Maximum rigor index occurred in up to three and nine hours for fish depurated in freshwater and saline environments, respectively. During the post-rigor period, the rigor index experienced a sharp drop in the freshwater environment, while in the saline environment it remained similar to that of the pre-depuration group. Depuration in a saline environment for up to seven days was efficient at maintaining of proximate composition and rigor mortis, however, depuration until the fifth day is indicated to maintain performance indices.

RESUMO: Nas últimas décadas, houve um aumento global na adoção de répteis como animais de companhia, principalmente tartarugas e jabutis. Considerando a popularidade dos répteis como animais de estimação no Brasil e a notável falta de dados sobre estafilococos potencialmente patogênicos nesses animais, o objetivo deste estudo foi isolar e avaliar a susceptibilidade antimicrobiana de espécies estafilocócicas de jabutis (Chelonoidis carbonaria) saudáveis no Brasil. Durante um período de 12 meses (fevereiro de 2019 a fevereiro de 2020), suabes cloacais de 66 jabutis saudáveis foram coletados no Centro de Triagem de Animais Silvestres em Belo Horizonte, Minas Gerais, Brasil. Os suabes foram plaqueados em ágar manitol salgado para isolamento de estafilococos e a identificação das espécies foi realizada usando MALDI-TOF MS. A susceptibilidade aos antimicrobianos foi investigada pelo método de difusão em disco, e a presença do gene mecA foi investigada por PCR para detectar resistência à meticilina. Dos animais testados, 72,7% foram positivos para o isolamento estafilocócico. Todos os isolados eram estafilococos coagulase-negativos (CoNS), sendo Staphylococcus sciuri (81,3%) e S. xylosus (12,5%) as espécies mais frequentemente isoladas. A maioria dos isolados (56%) foi resistente a pelo menos um antimicrobiano. Alta frequência de resistência foi observada para penicilina (35,5%) e tetraciclina (29,1%). Todas as estirpes foram sensíveis à cefoxitina, cloranfenicol, ciprofloxacina, eritromicina e gentamicina. Todos os isolados foram negativos para o gene mecA. O presente trabalho sugere que jabutis saudáveis são colonizados principalmente por CoNS, especialmente S. sciuri. Metade dos isolados foram resistentes a pelo menos um antimicrobiano, levantando questões sobre o possível papel desses animais como reservatórios de genes de resistência aos antimicrobianos.

ABSTRACT: In the last few decades, there has been a global increase in the adoption of reptiles as companion animals, mainly turtles and tortoises. Considering the popularity of reptiles as pets in Brazil, and a notable lack of data about potentially pathogenic staphylococci in these animals, this study isolated and evaluate the antimicrobial susceptibility of staphylococcal species from healthy tortoises (Chelonoidis carbonaria) in Brazil. During a 12-month period (February 2019 to February 2020), cloacal swabs from 66 healthy tortoises were collected at the Wild Animals Screening Center in Belo Horizonte, Minas Gerais, Brazil. The swabs were plated onto mannitol salt agar for staphylococci isolation, and species identification was performed using MALDI-TOF MS. Antimicrobial susceptibility was investigated using the disk diffusion method, and the presence of the mecA gene was investigated by PCR to detect methicillin resistance. Of the tested animals, 72.7% were positive for staphylococcal isolation. All isolates were coagulase-negative staphylococci (CoNS), and Staphylococcus sciuri (81.3%), and S. xylosus (12.5%) were the most frequently isolated species. The majority of the isolates (56%) were resistant to at least one antimicrobial agent. A high frequency of resistance was observed for penicillin (35.5%) and tetracycline (29.1 %). All strains were susceptible to cefoxitin, chloramphenicol, ciprofloxacin, erythromycin, and gentamicin. All isolates were negative for the mecA gene. The present work suggests that healthy tortoises are mainly colonized by CoNS, especially S. sciuri. Half of the isolates were resistant to at least one antimicrobial, raising questions regarding the possible role of these animals as reservoirs of antimicrobial resistance genes.