Resumo O cromo (VI), metal altamente tóxico, é um dos principais constituintes dos resíduos industriais. É liberado no solo e na água, causa problemas ambientais e de saúde de crescente preocupação pública em países em desenvolvimento como o Paquistão. O objetivo básico deste estudo foi o isolamento e a triagem de bactérias resistentes ao cromo de resíduos industriais coletados em Korangi e Lyari, Karachi (24˚52’46,0”N 66˚59’25,7”E e 24˚48’37,5”N 67˚06’52,6”E). Do total de 53 cepas isoladas, sete cepas bacterianas foram selecionadas por enriquecimento seletivo e identificadas com base em características morfológicas e bioquímicas. Essas cepas foram designadas como S11, S13, S17, S18, S30, S35 e S48, apresentaram alta resistência aos metais contra concentrações variáveis (100-1500 mg / l) de cromo. Já as cepas S35 e S48 foram identificadas por meio da sequência 16S rRNA e apresentaram 99% de similaridade com Bacillus paranthracis e Bacillus paramycoides. Além disso, as condições de crescimento incluindo temperatura e pH foram otimizadas e ambas as cepas bacterianas apresentaram crescimento máximo na temperatura de 30 ºC, enquanto seu pH ótimo foi observado em 7,5 e 6,5, respectivamente. Conclui-se que o potencial de resistência dessas bactérias resistentes ao cromo pode ser efetivamente utilizado na remoção de cromo de efluentes industriais contaminados. Técnicas de base biológica usando bactérias ajudarão a fornecer métodos mais baratos e ecológicos de remoção, recuperação e desintoxicação de cromo.

Abstract Chromium (VI) a highly toxic metal, a major constituent of industrial waste. It is continuously release in soil and water, causes environmental and health related issues, which is increasing public concern in developing countries like Pakistan. The basic aim of this study was isolation and screening of chromium resistant bacteria from industrial waste collected from Korangi and Lyari, Karachi (24˚52ʹ46.0ʺN 66˚59ʹ25.7ʺE and 24˚48ʹ37.5ʺN 67˚06ʹ52.6ʺE). Among total of 53 isolated strains, seven bacterial strains were selected through selective enrichment and identified on the basis of morphological and biochemical characteristics. These strains were designated as S11, S13, S17, S18, S30, S35 and S48, resistance was determined against varying concentrations of chromium (100-1500 mg/l). Two bacterial strains S35 and S48 showed maximum resistance to chromium (1600 mg/l). Bacterial strains S35 and S48 were identified through 16S rRNA sequence and showed 99% similarity to Bacillus paranthracis and Bacillus paramycoides. Furthermore, growth condition including temperature and pH were optimized for both bacterial strains, showed maximum growth at temperature 30ºC and at optimum pH 7.5 and 6.5 respectively. It is concluded that indigenous bacterial strains isolated from metal contaminated industrial effluent use their innate ability to transform toxic heavy metals to less or nontoxic form and can offer an effective tool for monitoring heavy metal contamination in the environment.

The potential of natural (N) and magnetized (M) forms of different rocks (calcite and dolomite) and clays (bentonite, kaolinite, and hematite) were evaluated for the removal of As(III) from aqueous solutions, in order to optimize and scrutinize the most suitable adsorbent. The order observed for efficiency of As(III) removal was N-calcite > N-dolomite > M-calcite > M-dolomite > N-bentonite > M-bentonite > M-kaolinite > N-kaolinite > M-hematite > N-hematite. On the basis of this analysis, natural calcite was further selected for in-depth analysis of various adsorption parameters such as time, pH, temperature, dosage, and concentration of As(III) ions. An excellent adsorption capacity of 19.05 mg·g-1 was displayed by calcite for As(III), which was significantly higher than that previously reported for other studies. The characterization of adsorbent calcite was performed by various analytical techniques: XRD, XRF, FTIR, SEM-EDS, TG/DTA and PZC. The kinetic investigation revealed that the adsorbent successfully removed over 98% of As(III) from aqueous solution after 160 minutes of equilibration time. The adsorption data was well fitted to the Freundlich isotherm model and fairly described by the pseudo-second-order mechanism. The mean energy of adsorption (E) determined from the Dubinin-Radushkevich (D-R) model was less than 8 kJ·mol-1, which indicates a physical adsorption process. The calculated thermodynamic parameters revealed that the adsorption of As(III) by calcite is endothermic, favourable and spontaneous.

Resumo A dengue é uma doença viral que é um sério problema de saúde das últimas décadas e a infecção transmitida através da picada de mosquito em humanos. Este estudo foi realizado para realizar a prevalência da dengue no distrito de Swabi. Foram coletadas 196 amostras de sangue de pacientes com faixa etária (0 a 80 anos) com dengue com base nos sintomas físicos do Complexo Médico Bacha Khan de Swabi, no período de agosto a outubro de 2017. Foram realizados testes sorológicos para detecção de IgM, IgG e NS1 (antígeno proteico não estrutural do vírus) contra a dengue. Do total de 196 casos confirmados de dengue, o sexo mais propenso foi o masculino 123 (62%), enquanto 73 (38%) eram do sexo feminino. Entre as faixas etárias; A faixa etária de 21 a 30 anos 62 (31,6%) foi a faixa etária mais predominante. As áreas altamente afetadas no distrito de Swabi foram Topi (40,8%), seguidas por Main Swabi (27%), Maneri (8,2%), Marghuz (6%), Shawa Ada (5,1%), Shah Mansoor (5,1%), Gohati (4,1%) e Chota Lahore (3,6%). Portanto, o departamento de saúde deve tomar ações educando o público sobre a limpeza básica do meio ambiente. A comunidade deve ser incentivada a participar do controle de tais doenças / infecções baseadas em vetores.

Abstract Dengue is a viral disease which is serious health concern from last few decades and the infection transmitted through mosquito bite into human. This study was conducted to carry out prevalence of dengue fever in District Swabi. A total of 196 blood sample were collected from patients with age ranges (0-80 years) having dengue fever on the basis of physical symptoms from Bacha Khan Medical Complex Swabi during August to October 2017. Serological test were performed for detection of IgM, IgG and NS1 (Non structural protein antigen of virus) against dengue. Out of total 196 confirmed dengue cases the most prone gender was male 123(62%) while 73(38%) were female. Among the age groups; 21-30 years group 62 (31.6%) was found the most predominated age group. The higly affected areas in district Swabi were Topi (40.8%) followed by Main Swabi (27%), Maneri (8.2%), Marghuz (6%), Shawa Ada (5.1%), Shah Mansoor (5.1%), Gohati (4.1%), and Chota Lahore (3.6%). Therefore, the health department should take actions by educating the public about basic cleanliness of the environment. The community should be encouraged to participate in the control of such vector based diseases/infections.

Green synthesis of nanoparticles has received a lot of attention from scientists globally because it is eco-friendly, relatively rapid, and a cost-effective method. This work presents a method for the green synthesis of nickel oxide nanoparticles (NiO-NPs) using leaf extracts of Populus ciliata as a reducing and stabilising agent. The synthesised NiO-NPs were characterised by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. It was found that the synthesised NiO-NPs shapes varied, were highly crystalline, and had a face-centred cubic geometry. The calculated crystallite size of the synthesised nanoparticles was 44 nm. Moreover, the antibacterial activity of the synthesised NiO-NPs was also conducted against Gram-positive bacteria (Bacillus subtilis, Bacillus licheniformis) and Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae). Bacillus subtillus showed a maximum inhibition zone of 28.1±1.6 mm, whereas Klebsiella pneumonia showed a minimum inhibition zone of 9.2±0.5 mm. It was also found that the antibacterial activity increased with an increase in the concentration of NiO-NPs.