Resumo: Elucidar os mecanismos fisiológicos e ecológicos de dormência e germinação de sementes é de grande importância para a conservação e uso de espécies vegetais. Baseando-se no sistema de classificação de Baskin e Baskin para dormência de sementes, neste trabalho foram estudadas as causas da dormência em sementes de Tarenaya hassleriana (Cleomaceae), utilizando-se temperaturas alternadas, estratificação úmida fria, armazenamento seco e tratamento de imersão em GA3. Os resultados indicaram que as sementes maduras frescas de T. hassleriana tinham dormência combinada, incluindo dormência física e dormência fisiológica não profunda tipo 2, e eram fotoblásticas, com temperatura ótima de germinação de 35 °C. Além disso, sementes maduras frescas de T. hassleriana podem ter a dormência eficientemente quebrada e germinar por temperatura alternada de 20 °C/30 °C, estratificação úmida fria e estratificação úmida fria após armazenamento seco. Além disso, o tratamento de imersão com GA3 também pode quebrar a dormência e subsequente germinação a 35 °C, e o tratamento de armazenamento a seco pode quebrar a dormência e subsequente germinação a 5-15 °C. Esses resultados também sugerem que houve interações complexas entre o fitohormônio, o potencial osmótico e as vias regulatórias de sinalização de temperatura durante a liberação de dormência e germinação de sementes de T. hassleriana, que merecem um estudo mais aprofundado. Resumo vegetais Baseandose Baseando se Cleomaceae, Cleomaceae , (Cleomaceae) utilizandose utilizando alternadas GA T combinada 2 fotoblásticas 3 C °C disso C30 30 °C/3 515 5 15 5-1 fitohormônio aprofundado (Cleomaceae C3 °C/ 51 1 5-

Abstract: Elucidating the physiological and ecological mechanisms of seed dormancy and germination is of great significance for species conservation and the application of plant resources. Based on Baskin and Baskin’s classification system for seed dormancy, the cause of dormancy in Tarenaya hassleriana (Cleomaceae) seeds was studied using alternating temperature, cold moist stratification, dry storage, and GA3 soaking treatment. The results indicated that fresh mature T. hassleriana seeds had a combinational dormancy, including a physical dormancy and a type 2 non-deep physiological dormancy, and were photoblastic, with an optimal germination temperature of 35°C. In addition, fresh mature T. hassleriana seeds may be efficiently released from dormancy and promoted to germinate by an alternating temperature of 20 °C/30 °C, cold moist stratification, and cold moist stratification following dry storage. Furthermore, GA3 soaking treatment could also promote dormancy release and subsequent germination at 35 °C, and dry storage treatment could promote dormancy release and subsequent germination at 5-15 °C. These results also suggested that there were complex cross-talks among phytohormone, osmotic potential, and the temperature signaling regulatory pathways during dormancy release and germination of T. hassleriana seeds, which deserve further study. Abstract resources Baskins s Cleomaceae (Cleomaceae GA T nondeep non deep photoblastic 35C C 35°C addition C30 30 °C/3 °C Furthermore 3 515 5 15 5-1 crosstalks cross talks phytohormone potential study C3 °C/ 51 1 5-

RESUMEN Introducción: El páncreas libera insulina para ayudar al cuerpo humano a utilizar la glucosa en sangre. Regula el metabolismo, favoreciendo la absorción de la glucosa por la sangre. Objetivo: El objetivo de este trabajo fue crear un biosensor electroquímico basado en un nanomaterial de grafeno magnético para medir los niveles de insulina en la sangre de los deportistas. Método: Sobre un electrodo de carbono vítreo, se sintetizó un nanocompuesto de grafeno magnético creado por óxido de grafeno (GO) y óxidos bimetálicos de Fe-Ni mediante el método de deposición electroquímica (GCE). Resultados: La deposición eléctrica inmediata de las nanopartículas de óxido bimetálico Fe-Ni con forma esférica sobre la nano plancha de GO sin agregaciones fue validada por las caracterizaciones estructurales de Fe-Ni/GO/GCE mediante XRD y SEM. Los resultados electroquímicos para la determinación de la insulina mostraron una buena sensibilidad y capacidad anti-interferencia. La aplicabilidad y la precisión del sensor electroquímico propuesto para detectar la insulina se exploraron con muestras de suero sanguíneo de deportistas. Conclusión: Los resultados asignados a valores aceptables de RSD (3,31% a 4,30%) confirmaron la viabilidad del sensor propuesto para detectar la insulina en sangre de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción metabolismo Objetivo deportistas Método vítreo (GO FeNi Fe Ni GCE. GCE . (GCE) Resultados FeNi/GO/GCE FeNiGOGCE Ni/GO/GCE SEM antiinterferencia. antiinterferencia anti interferencia. interferencia anti-interferencia Conclusión 3,31% 331 3 31 (3,31 4,30% 430 4 30 atletas II tratamiento (GCE NiGOGCE 3,31 33 (3,3 4,30 43 3,3 (3, 4,3 3, (3 4, (

RESUMO Introdução: A insulina é liberada pelo pâncreas para auxiliar o corpo humano na utilização da glicose sanguínea. Ela regula o metabolismo, promovendo a absorção da glicose pelo sangue. Objetivo: O objetivo deste trabalho foi criar um biossensor eletroquímico baseado em nanomaterial de grafeno magnético para medir os níveis de insulina no sangue dos atletas. Método: Em um eletrodo de carbono vítreo, um nanocomposto magnético de grafeno criado por óxido de grafeno (GO) e óxidos bimetálicos de Fe-Ni foi sintetizado usando o método de deposição eletroquímica (GCE). Resultados: A deposição elétrica imediata de nanopartículas de óxido bimetal Fe-Ni com a forma esférica na nano folha GO sem agregações foi validada por caracterizações estruturais de Fe-Ni/GO/GCE utilizando XRD e SEM. Os resultados eletroquímicos para determinação da insulina demonstraram boa sensibilidade e capacidade anti-interferência. A aplicabilidade e precisão do sensor eletroquímico proposto para detectar insulina foram exploradas por amostras de soro sanguíneo dos esportistas. Conclusão: Os resultados designados para os valores aceitáveis de RSD (3,31% a 4,30%) confirmaram a viabilidade do sensor proposto para detecção de insulina sanguínea de atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução metabolismo Objetivo atletas Método vítreo (GO FeNi Fe Ni GCE. GCE . (GCE) Resultados FeNi/GO/GCE FeNiGOGCE Ni/GO/GCE SEM antiinterferência. antiinterferência anti interferência. interferência anti-interferência esportistas Conclusão 3,31% 331 3 31 (3,31 4,30% 430 4 30 II tratamento (GCE NiGOGCE 3,31 33 (3,3 4,30 43 3,3 (3, 4,3 3, (3 4, (

ABSTRACT Introduction: The pancreas releases insulin to assist the human body in utilizing blood glucose. It regulates metabolism by promoting the absorption of glucose into the blood. Objective: This work aimed to create an electrochemical biosensor based on magnetic graphene nanomaterial to measure insulin levels in athletes’ blood. Method: A magnetic graphene nanocomposite created by graphene oxide (GO) and Fe-Ni bimetallic oxides on a glassy carbon electrode was synthesized using the electrochemical deposition method (GCE). Results: The immediate electrical deposition of Fe-Ni bimetallic oxide nanoparticles with the spherical shape on the GO nanosheet without aggregations was validated by structural characterizations of Fe-Ni/GO/GCE using XRD and SEM. The electrochemical results for insulin determination showed good sensitivity and anti-interference capability. The applicability and accuracy of the proposed electrochemical sensor to detect insulin were explored by blood serum samples from sportsmen. Conclusion: The results assigned acceptable RSD values (3.31% to 4.30%) and confirmed the feasibility of the proposed sensor for detecting athletes’ blood insulin. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction Objective athletes Method (GO FeNi Fe Ni GCE. GCE . (GCE) Results FeNi/GO/GCE FeNiGOGCE Ni/GO/GCE SEM antiinterference anti interference capability sportsmen Conclusion 3.31% 331 3 31 (3.31 4.30% 430 4 30 II outcomes (GCE NiGOGCE 3.31 33 (3.3 4.30 43 3.3 (3. 4.3 3. (3 4. (

ABSTRACT Mastering accurate spatial planting and distribution status of the crops is significantly important for the nation to guide the agricultural production and formulate agricultural policies from a macro perspective. In this paper, the Landsat-8 OLI satellite images were taken as the data sources. And as for the nine crop types within the study area, such as the wheat, rice, and other crops, three classification methods of the random forest classification (RFC), the support vector machine (SVM), and the maximum likelihood classification (MLC) were applied in extracting the planting area of winter wheat in Wushi County of Xinjiang Uygur Autonomous Region. It can be seen from the results that, general classification accuracy of MLC, SVM, and RFC are respectively 80.58%, 87.95%, and 95.96%, while their Kappa coefficients are respectively 0.61, 0.76, and 0.86. The RFC method shows higher classification accuracy that those of MLC and SVM methods. The principal component analysis (PCA) was carried out on the original 7-band image to extract the first 4 principal components and calculate the normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), wide dynamic range vegetation index (WDRVI), and normalized difference water index (NDWI). Meanwhile, the 6 additional auxiliary feature bands were superimposed on the original 7-band images to carry out reclassification, through which, the general accuracy of MLC increased by 3 percent while its Kappa coefficient increased by 0.06; the SVM general accuracy increased by 3.02 percent while its Kappa coefficient increased by 0.13; and the general accuracy of the RFC increased by 0.85 percent while its Kappa coefficient increased by 0.02. This indicates that, the adding of auxiliary information can improve the crop classification and identification ability and accuracy. Based on the comprehensive evaluation, the classification method of random forest is proved to have better performance in winter wheat identification.

Abstract Temperate japonica rice plays a very important role in food security. In this study, a set of 191 temperate japonica accessions from 30 countries were planted in two sites in China, and 12 agronomic traits were measured. The results showed a wide range of variation for the traits measured. Most of the accessions are short; three accessions with large panicles and seven accessions with large grains were identified. Sixty-two quantitative trait loci (QTLs) were identified for 12 traits that were measured. Among them, twenty-one QTLs were identified in both experiments, and 41 QTLs were identified in only one site. Some known genes are located in the QTL regions identified in our study. SNP markers for grain size were identified and could be efficiently used for breeding selection. This study provided useful information for future gene validation and marker assisted selection for some important agronomic traits of temperate japonica rice.

OBJECTIVE: Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. METHODS: Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. RESULTS: Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p<0.001). Circulating miR-424 levels were reduced 3.6-fold in stable coronary artery disease patients (1.18 ± 0.60) and 5-fold in unstable coronary artery disease patients (0.86 ± 0.54) compared with non-coronary artery disease patients (4.35 ± 2.20) (p<0.001). In contrast, circulating miR-765 levels were elevated 3.98-fold in stable coronary artery disease patients (6.09 ± 2.27) and 5.33-fold in unstable coronary artery disease patients (8.17 ± 2.77) compared with non-coronary artery disease patients (1.53 ± 0.99) (p<0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve for circulating miR-149, miR-424 and miR-765 were 0.938, 0.919 and 0.968 in stable CAD patients and 0.951, 0.960 and 0.977 in unstable coronary artery disease patients compared with non-coronary artery disease patients. CONCLUSION: Our results suggest that circulating miR-149, miR-424 and miR-765 might be novel, non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged patients. However, future prospective trials in large patient cohorts are necessary before reaching a solid conclusion.