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Potential of chitosan and activated carbon biocomposites for removing enrofloxacin and oxytetracycline from water
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Molina-Amaya, José Manuel

; Ávila-Reyes, José Antonio

; Delgado-Alvarado, Eli Amanda

; González-Valdez, Laura Silvia

; Torres-Ricario, Rene

; Rojas-López, Marlon

; Almaraz-Abarca, Norma

; Gutiérrez-Velázquez, Marcela Verónica

Revista internacional de contaminación ambiental

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2024, Volume 40 elocation 55051

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN La enrofloxacina (ENRO) y la oxitetraciclina (OTC) son dos antibióticos difíciles de eliminar por métodos convencionales de tratamiento de agua. Las características de los biocompositos sugieren que podrían ser una alternativa para la remoción de este tipo de sustancias por procesos de adsorción. El objetivo del presente estudio fue determinar y comparar el potencial de los biocompositos formados con quitosano y diferentes concentraciones de carbón activado (CA) con un tamaño de partícula de 500 nm, preparados a partir de bagazo de agave, para remover enrofloxacina y oxitetraciclina en solución acuosa. Se evaluó la efectividad de la adición de diferentes concentraciones de CA (0, 0.5, 2 y 5 % p/v) al quitosano mediante análisis morfológico usando microscopía electrónica de barrido. La detección de los grupos funcionales característicos llevada a cabo mediante espectroscopía infrarroja por transformada de Fourier (FTIR), mostró que las nanopartículas de CA están unidas al biocomposito de quitosano sugiriendo un entrecruzamiento químico estable, formado durante el proceso de polimerización. La prueba de eliminación de ENRO (10 mg/mL) y OTC (10 mg/mL) en agua se realizó mediante un método en gradiente de cromatografía líquida de alta resolución (HPLC). Los resultados mostraron que la adición de CA al quitosano formó un biocomposito con capacidad máxima de remover 59.3 % de OTC (tiempo de contacto 2 h a 100 rpm, 4 g biocomposito/L solución, pH 6-7, 30 ºC) y un porcentaje 50.3 % como remoción máxima de ENRO (2 h tiempo de contacto a 100 rpm, 4 g biocomposito/L solución, pH 6, 30 ºC). Los biocompositos formados por quitosano y carbón activado de agave pueden apoyar el desarrollo de herramientas para la eliminación de antibióticos recalcitrantes de los cuerpos de agua.

ABSTRACT Enrofloxacin (ENRO) and oxytetracycline (OTC) are two antibiotics, which are difficult to remove by conventional water treatment methods. The characteristics of the biocomposites suggest that they could represent an alternative for the removal of this type of substances through adsorption processes. The current study aimed to determine and compare the potential of biocomposites formed with chitosan and different concentrations of activated carbon (AC) with a particle size of 500 nm, prepared from agave bagasse, to remove enrofloxacin and oxytetracycline solved in water. The effectiveness of the addition of different concentrations of AC (0, 0.5, 2 and 5% w/v) to chitosan was evaluated by morphological analysis using scanning electron microscopy (SEM). The detection of the characteristic functional groups carried out by Fourier transform infrared spectroscopy (FTIR) indicated that the nanoparticles of AC were attached in the chitosan biocomposite, and stable chemical crosslinking was formed during the polymerization process. The removal test of ENRO (10 mg/mL) and OTC (10 mg/mL) in a water was performed by a gradient method of high-performance liquid chromatography (HPLC). The results showed that the addition of AC to chitosan formed a biocomposite with maximum capacity to remove 59.3% of OTC (contact time 2 h at 100 rpm, 4 g biocomposite/L solution, pH 6-7, 30 ºC), and a maximum removal of 50.3% of ENRO (contact time 2 h at 100 rpm, 4 g biocomposite/L solution, pH 6, 30 ºC). Biocomposites formed by chitosan and agave activated carbon can support the development of tools for the removal of recalcitrant antibiotics from water bodies.

https://doi.org/10.20937/rica.55051

Cross-cultural invariance of the Spanish version of the COVID-19 Assessment Scorecard to measure the perception of government actions against COVID-19 in Latin America Crosscultural Cross cultural COVID19 COVID 19 COVID-1 COVID1 1 COVID-
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Caycho-Rodríguez, Tomás

; Valencia, Pablo D.

; Ventura-León, José

; Carbajal-León, Carlos

; Vilca, Lindsey W.

; Reyes-Bossio, Mario

; Delgado-Campusano, Mariel

; Yupanqui-Lorenzo, Daniel E.

; Paredes-Angeles, Rubí

; Rojas-Jara, Claudio

; Gallegos, Miguel

; Cervigni, Mauricio

; Martino, Pablo

; Polanco-Carrasco, Roberto

; Palacios, Diego Alejandro

; Moreta-Herrera, Rodrigo

; Samaniego-Pinho, Antonio

; Rivera, Marlon Elías Lobos

; Figares, Andrés Buschiazzo

; Puerta-Cortés, Diana Ximena

; Corrales-Reyes, Ibraín Enrique

; Calderón, Raymundo

; Gallegos, Walter L. Arias

; Petzold, Olimpia

; Camargo, Andrés

; Torales, Julio

; Blanco, J. Arkangel Monge

; González, Pedronel

; Smith-Castro, Vanessa

; Rivera, Wendy Yamilet Matute

; Ferrufino-Borja, Daniela

; Ceballos-Vásquez, Paula

; Muñoz-del-Carpio-Toia, Agueda

; Palacios, Jorge

; Burgos-Videla, Carmen

; León, Ana María Eduviges Florez

; Vergara, Ibeth

; Vega, Diego

; Barria-Asenjo, Nicol A.

; Schulmeyer, Marion K.

; Rios, Hassell Tatiana Urrutia

; Lira, Arelly Esther Lira

Psicologia: Reflexão e Crítica

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2023, Volume 36 elocation 34

Resumo: En | Texto: En | PDF: En

Abstract Objectives The present study aimed to evaluate the measurement invariance of a general measure of the perception of governmental responses to COVID-|19 (COVID-SCORE-10) in the general population of 13 Latin American countries. Methods A total of 5780 individuals from 13 Latin American and Caribbean countries selected by non-probabilistic snowball sampling participated. A confirmatory factor analysis was performed and the alignment method was used to evaluate invariance. Additionally, a graded response model was used for the assessment of item characteristics. Results The results indicate that there is approximate measurement invariance of the COVID-SCORE-10 among the participating countries. Furthermore, IRT results suggest that the COVID-SCORE-10 measures with good psychometric ability a broad spectrum of the construct assessed, especially around average levels. Comparison of COVID-SCORE-10 scores indicated that participants from Cuba, Uruguay and El Salvador had the most positive perceptions of government actions to address the pandemic. Thus, the underlying construct of perception of government actions was equivalent in all countries. Conclusion The results show the importance of initially establishing the fundamental measurement properties and MI before inferring the cross-cultural universality of the construct to be measured. COVID|19 COVID19 COVID |19 19 COVID-|1 COVIDSCORE10 COVIDSCORE SCORE 10 (COVID-SCORE-10 1 578 nonprobabilistic non probabilistic participated Additionally characteristics COVID-SCORE-1 Furthermore assessed levels Cuba pandemic Thus crosscultural cross cultural measured COVID|1 COVID1 |1 COVID-| COVIDSCORE1 (COVID-SCORE-1 57 COVID-SCORE- COVID| | COVID- (COVID-SCORE- 5 COVID-SCORE (COVID-SCORE

https://doi.org/10.1186/s41155-023-00277-9

Sea slugs (Gastropoda: Heterobranchia) from Rio Grande do Norte, Northeastern Brazil
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Delgado, Marlon

; Freire, Fúlvio Aurélio de Morais

; Meirelles, Carlos Augusto Oliveira de

; D’Oliveira, Rosângela Gondim

; Padula, Vinicius

; Bahia, Juliana

; Brandão, Simone Nunes

Papéis Avulsos de Zoologia

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2022, Volume 62 elocation e202262063

Resumo: En | Texto: En | PDF: En

Abstract Heterobranch sea slugs (Gastropoda) present reduction, internalization, or absence of shell, and include more than 6,000 described species. Approximately 250 species are recorded from Brazil but only 14 had been previously recorded from Rio Grande do Norte, on the Brazilian northeastern coast. As a result of different expeditions conducted between 2008 and 2020, 41 species were collected and identified. Among them, five species were recorded for the first time from the South Atlantic Ocean: Chelidonura hirundinina (Quoy & Gaimard, 1833); Sclerodoris prea (Ev. Marcus & Er. Marcus, 1967); Thuridilla malaquita Ortea & Buske, 2014; Berthella nebula Ghanimi, Schrödl, Goddard, Ballesteros, Gosliner & Valdés, 2020 and Berthella vialactea Ghanimi, Schrödl, Goddard, Ballesteros, Gosliner & Valdés, 2020. Thirty-six species are recorded for the first time from Rio Grande do Norte. The present survey expands the knowledge of the diversity of sea slugs from Brazil and supports the hypothesis of similarity between the heterobranch sea slug fauna from the northeastern Brazil and the Caribbean Sea.

https://doi.org/10.11606/1807-0205/2022.62.063

Impacto en la morbimortalidad por COVID-19 según las regulaciones del uso de mascarilla
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Saavedra-Delgado, Marlon E.

; Villaseñor-Todd, Ana

; Caicedo-Agudelo, Sandra P.

; Lázaro-Presenda, David A.

; Ng-Solís, Bernardo

Gaceta médica de México

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Jun 2021, Volume 157 Nº 3 Páginas 288 - 295

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Introduction: Currently, the face mask represents an incomparable symbol whose value went from clinical work to impacting the containment of the spread of SARS-CoV-2, although it has become an element of discord in the general population. Objective: To establish the impact of face mask use policies on COVID-19 morbidity and mortality. Methods: Face mask use policy variables, obtained from the website of the #Masks4All scientific movement, were associated with the number of infections, deaths and flattening of the curve published by the Johns Hopkins University resource center and EndCoronavirus.org. Results: Face mask use policies were universal (required in shops, restaurants, public transport), partial (recommended, required in any public place) and absent. Associations of the face mask use policy with total cases (p = 0.01), cases per million (p = 0.04) and deaths per million population (p = 0.02) were statistically significant. Associations of the variables with the epidemiological curve trend were also statistically significant (p = 0.00). Conclusion: The recommendation for face mask widespread use is a measure with sufficient scientific support to reduce the number of COVID-19-related infections and deaths.

Resumen Introducción: Actualmente, la mascarilla representa un símbolo incomparable cuyo valor pasó del trabajo clínico a impactar en la contención de la propagación del virus SARS-CoV-2; se convirtió en un elemento de discordia en la población general. Objetivo: Establecer el impacto de las políticas de uso de mascarilla en la morbimortalidad por COVID-19. Métodos: Se asociaron las variables de política de uso de cubrebocas, obtenidas de la página web del movimiento científico #Masks4All, con el número de contagios, muertes y aplanamiento de la curva publicados por el centro de recursos de la Universidad Johns Hopkins y EndCoronavirus.org. Resultados: Las políticas de uso de mascarilla fueron de tipo universal (requerido en tiendas, restaurantes, transporte público), parcial (recomendado, requerido en cualquier lugar público) y ausente. Las asociaciones de la política de uso de mascarilla con casos totales (p = 0.01), casos por millón (p = 0.04) y muertes por millón de habitantes (p = 0.02) resultaron estadísticamente significativas. Las asociaciones de las variables con la tendencia de la curva epidemiológica también resultaron estadísticamente significativas (p = 0.00). Conclusión: La recomendación del uso generalizado de mascarilla es una medida con suficiente respaldo científico para reducir el número de contagios y muertes por COVID-19.

https://doi.org/10.24875/gmm.20000801 388 downloads

Biomedical applications of polymeric biomaterials
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Osorio-Delgado, Marlon Andrés

; Henao-Tamayo, Leydi Johanna

; Velásquez-Cock, Jorge Andrés

; Cañas-Gutierrez, Ana Isabel

; Restrepo-Múnera, Luz Marina

; Gañán-Rojo, Piedad Felisinda

; Zuluaga-Gallego, Robín Octavio

; Ortiz-Trujillo, Isabel Cristina

; Castro-Herazo, Cristina Isabel

DYNA

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Jun 2017, Volume 84 Nº 201 Páginas 241 - 252

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Los materiales poliméricos han tenido una imagen desfavorable, ya que son asociados con contaminación. Sin embargo, estos son de gran importancia para la biomedicina. Los polímeros son materiales muy versátiles, se pueden obtener de múltiples configuraciones químicas y mezclas para generar materiales compuestos con propiedades sinérgicas. En esta investigación se realizó un análisis científico y tecnológico de las aplicaciones biomédicas de biomateriales poliméricos y se recopila algunas de sus aplicaciones, propiedades mecánicas y características importantes para la industria biomédica. Los biomateriales poliméricos son una temática de punta como se refleja en el número de artículos científicios y de patentes. En la actualidad, estos biomateriales pueden llegar a reemplazar, reforzar o cumplir una función específica en el cuerpo humano. No obstante, debido a la complejidad de los sistemas biológicos aún se siguen presentando reacciones inmunes, que evitan el desarrollo de tejidos u órganos funcionales a escala de laboratorio.

Abstract Polymer biomaterials have had an unfavourable image due to their association with pollution. However, they are important in biomedicine. Polymers are versatile materials, they can be obtained in several chemical configurations and mixtures to generate composite materials with synergistic properties. In this research, a scientific and technological analysis of the biomedical application of polymeric biomaterials was performed, along with the compilation of their applications, production, mechanical properties and important features for the biomedical industry. Polymeric biomaterials are a cutting-edge topic, as reflected in the number of scientific articles and patents. Nowadays, these biomaterials can replace, reinforce or fulfil a specific function in the human body. However, due to the complexity of biological systems, immune reactions are still present, preventing the development of tissues and functional organs in laboratory scale.

https://doi.org/10.15446/dyna.v84n201.60466 10978 downloads

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
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Mata-Miranda, Monica Maribel

; Vazquez-Zapien, Gustavo Jesus

; Rojas-Lopez, Marlon

; Sanchez-Monroy, Virginia

; Perez-Ishiwara, David Guillermo

; Delgado-Macuil, Raul Jacobo

Biological Research

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2017, Volume 50 elocation 14

Resumo: En | Texto: En | PDF: En

Abstract Background Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. Results In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way. Conclusion Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.

https://doi.org/10.1186/s40659‑017‑0119‑6 830 downloads

Application of Infrared Spectroscopy to the Monitoring of Lactose and Protein From Whey After Ultra and Nano Filtration Process
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Solís-Oba, Myrna

; Teniza-García, Ogilver

; Rojas-López, Marlon

; Delgado-Macuil, Raúl

; Díaz-Reyes, Joel

; Ruiz, Rosario

Journal of the Mexican Chemical Society

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Set 2011, Volume 55 Nº 3 Páginas 190 - 193

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Whey is produced during cheese manufacture, some of its constituents are lactose and proteins. In different countries such constituents are separated for use as raw material; some of the processes to separate these components are by ultra and nano-filtration, however most common methods for the determination of lactose and proteins are not accurate. This paper shows that infrared spectroscopy is a good alternative for the quantification of lactose and proteins after ultra- and nano-filtration processes. Linear calibration curves were obtained with this analytical technique for aqueous solutions containing lactose or protein in the range 0 to 20%; after 20% lactose, the solution becomes saturated. Infrared spectroscopy is a rapid and precise method that could be successfully used to quantify these compounds and follow the ultra- and nano-filtration process applied to purify lactose and proteins from whey.

El suero se produce durante la elaboración de queso, algunos de sus componentes son lactosa y proteínas. En diferentes países estos constituyentes son separados para usarse como materias primas; algunos procesos usados para su separación son por ultra y nano filtración, sin embargo, la mayoría de los métodos comerciales para cuantificar lactosa y proteínas no son exactos. En este trabajo se demuestra que la espectroscopía infrarroja es un buen método para cuantificar lactosa y proteína después de los procesos de ultra y nano filtración. Las curvas de calibración que se prepararon con esta técnica analítica midiendo soluciones acuosas de lactosa y proteína en el intervalo de 0 a 20%) de estos componentes, mostraron buena linealidad; después del 20%> la solución de lactosa se satura. La espectroscopia infrarroja mostró ser un método rápido y preciso que puede ser usada para cuantificar esos compuestos y dar seguimiento a los procesos de ultra y nano filtración aplicados para purificar lactosa y proteínas del suero.

2935 downloads

In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)
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Valdez-Melara, Marta

; García, Alexander

; Delgado, Marlon

; Gatica-Arias, Andrés M.

; Ramírez-Fonseca, Pilar

Revista de Biología Tropical

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Nov 2009, Volume 57 Páginas 119 - 127

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

En este estudio se desarrolló un método eficiente y reproducible para la regeneración de líneas puras de la planta tropical Cucurbita moschata mediante la vía de embriogénesis somática. Además se investigó acerca de la influencia del genotipo, transplante, y la concentración de N6-benzylaminopurina (BAP), 2,4-diclorofenoxyacetico ácido (2,4-D) y 2,4,5-triclorofenoxyacetico ácido (2,4,5-T) en la inducción de embriogénesis somática. Los callos embriogenéticos viables fueron producidos de embriones zigóticos (53-56%) y cotiledones de semillas (70%) de C. moschata cv. Sello de Oro cultivados en un medio de inducción de callos (CIM) suplementado con 0.5 mg/l o 3.5 mg/l 2,4-D. Los callos no embriogénicos fueron obtenidos de segmentos de hojas de C. moschata cv. Sello de Oro cultivados con CIM suplementado con diferentes concentraciones de BAP y 2,4-D y cotiledones de semillas de C. moschata cv. PVG 04 cultivado con CIM con BAP y 2,4,5-T. La inducción de callos embriogenéticos fue exitosa en un 75% de las líneas puras evaluadas de C. moschata y el porcentaje de frecuencia de los callos fue de 5% a 34%. Se logró una adecuada aclimatización de las plantas in vitro tanto en el invernadero como en el campo. Las plantas regeneradas fueron normales morfológicamente, y las flores y frutos poseen semillas que pueden germinar normalmente.

An efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally. Rev. Biol. Trop. 57 (Suppl. 1): 119-127. Epub 2009 November 30.

1664 downloads

Fijación anterior de la columna cervical con cesta roscable de titanio: Experiencia en 50 casos
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Krivoy, Saúl

; Wallens, Adelaida

; Díaz, Marlon

; Delgado, Ilse

Gaceta Médica de Caracas

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Abr 2004, Volume 112 Nº 2 Páginas 116 - 123

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Las enfermedades cervicales discales y degenerativas son de las más frecuentes en la práctica médica. En pacientes menores de 40 años es más común la hernia discal; en mayores de 40 años, lo son las enfermedades degenerativas. Se analizan los procedimientos diagnósticos más frecuentemente utilizados y los criterios para la selección del tratamiento quirúrgico. Se presenta la experiencia del autor en 50 casos de patología cervical discal y degenerativa tratados por abordaje anterior con discoidectomía y fusión utilizando la cesta roscada de titanio sin uso de injertos óseos, placas o tornillos. Las principales ventajas de esta técnica son: acortamiento del procedimiento quirúrgico; menor instrumentación; estabilización inmediata de la columna cervical con recuperación y deambulación precoz de los pacientes y su retorno a actividades normales en un lapso de dos semanas, con mínimas complicaciones y reducción de costos.

Cervical spinal degenerative and discal diseases are among the most common conditions encountered in medical practice. Herniated discs are more common in patients under 40 years of age and degenerative processes are more comon after that age. In this study, the most common diagnostic methods and the current criteria for surgical management are reviewed. The author presents his experience of 50 cases with cervical discal and degenerative disease treated by discectomy and fusion through anterior approach using the titanium cage without use of bone grafts, plates or screws. The main advantages of this technique include shortening of surgical time and fewer instrumentation, immediate stabilization of the cervical spine, early functional recovery and return to normal activities within two weeks with minimal complications, and lower cost.

1274 downloads Citado 1 vez em SciELO

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Glossário e ajuda para busca

Você pode enriquecer sua busca de uma forma muito simples. Use os índices de pesquisa combinados com os conectores (AND ou OR) e especifique cada vez mais sua busca.

Por exemplo, se você deseja buscar artigos sobre
casos de dengue no Brasil em 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

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