Silver film is commonly used to examine the structure and composition of molecules due to its surface-enhanced Raman scattering (SERS) effect. A simple method for preparing SERS silver films is proposed in this paper by electrodeposition in an amide-based liquid mixture (urea-acetamide-LiBr). The electrochemical behavior of silver in urea-acetamide-LiBr mixture was studied by cyclic voltammetry (CV) and chronoamperometry. It was found that the nucleation mechanism of silver shifts to instantaneous nucleation under diffusion control with increasing overpotential and temperature. The energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD) investigations confirmed that the obtained deposits are pure silver. Scanning electron microscopy (SEM) analysis showed that the morphology of the silver deposits depends on the deposition potential and temperature. Rhodamine 6G was used as probe molecules for SERS experiments, showing that these new active substrates have high sensitivity to SERS response with high enhancement factors, of approximately 107, and enable Raman scattering even for diluted solutions. surfaceenhanced surface enhanced (SERS effect amidebased amide based ureaacetamideLiBr. ureaacetamideLiBr urea acetamide LiBr . (urea-acetamide-LiBr) CV (CV chronoamperometry temperature EDS (EDS Xray X ray XRD (XRD SEM (SEM G experiments factors 107 solutions (urea-acetamide-LiBr 10 1

This research aims to develop a precise and efficient dispersive solid-phase microextraction (DSPE) technique using third-generation polyamidoamine (PAMAM) dendrimer-grafted nanoparticles to quantify benzoylurea pesticides in green tea, black tea, and oolong tea infusions. The extraction efficiency is optimized through single-factor adjustments of key parameters, including adsorbent quantity (5-20 mg), adsorption duration (5-20 min), desorbent identity (methanol, acetonitrile), volume (100-600 μL), sodium chloride concentration (0-10%, m/v), and sample solution pH (3-8). The optimized method demonstrates linearity in the range of 2-500 μg L-1, with limits of detection (LODs) ranging from 0.6-1.5 μg L-1 and limits of quantification (LOQs) from 2.0 5.0 μg L-1. Recovery rates of 81.2-96.0% are achieved, with intra-day relative standard deviations (RSDs) of 1.4-2.8% and inter-day relative standard deviations of 2.0-3.9%, indicating high accuracy and repeatability. The successful application of detecting residues in commercial tea samples underscores the strong potential of the technique to determine benzoylurea analyte levels. solidphase solid phase DSPE (DSPE thirdgeneration third generation PAMAM (PAMAM dendrimergrafted dendrimer grafted infusions singlefactor single factor parameters 520 5 20 (5-2 mg, mg , mg) min, min min) methanol, methanol (methanol acetonitrile, acetonitrile acetonitrile) 100600 100 600 (100-60 μL, μL μL) 010%, 010 0 10%, 10 (0-10% m/v, mv m/v m v m/v) 38. 38 3 8 . (3-8) 2500 2 500 2-50 L1, L1 L 1, 1 LODs (LODs 0.61.5 0615 0.6 1.5 6 0.6-1. L- LOQs (LOQs 2. 50 5. L1. 1. 81.296.0% 812960 81.2 96.0% 81 96 81.2-96.0 achieved intraday intra day RSDs (RSDs 1.42.8% 1428 1.4 2.8% 4 1.4-2.8 interday inter 2.03.9%, 2039 3.9%, 9 2.0-3.9% repeatability levels 52 (5- 10060 60 (100-6 010% 01 10% (0-10 (3-8 250 2-5 61 0.61. 061 06 0. 15 0.6-1 296 81.296.0 81296 812 81. 960 96.0 81.2-96. 42 1.42.8 142 14 28 2.8 1.4-2. 03 2.03.9% 203 39 3.9% 2.0-3.9 (5 1006 (100- (0-1 (3- 25 2- 0.61 0.6- 29 81.296. 8129 96. 81.2-96 1.42. 1.4-2 2.03.9 3.9 2.0-3. ( (100 (0- (3 81.296 81.2-9 1.42 1.4- 2.03. 3. 2.0-3 (10 (0 81.29 81.2- 2.03 2.0- (1

Abstract Background Atrial fibrillation (AF) and heart failure (HF) frequently coexist, resulting in adverse outcomes. However, controversies remain regarding the efficacy of catheter ablation (CA) in AF patients with severe left ventricular dysfunction. Objectives The purpose of this study was to perform a meta-analysis of prospective randomized controlled trials to evaluate the efficacy of CA versus medical therapy (MT) in AF patients with left ventricular ejection fraction (LVEF) ≤45%. Methods We searched the literature for studies that compared CA to MT in AF patients with LVEF ≤45%. A meta-analysis of 7 clinical trials was performed, including 1163 patients with AF and HF. Subgroup analysis was performed based on baseline LVEF. All tests were 2-sided; only the p-value <0.05 was considered statistically significant. Results We found that CA was associated with lower all-cause mortality (risk ratio: 0.52, 95% CI: 0.37 to 0.72; p<0.01) and greater improvements in LVEF (mean difference: 4.80%, 95% CI: 2.29% to 7.31%; p<0.01) compared to MT. Patients in the CA group had a lower risk of HF hospitalization and AF recurrence and a significantly better quality of life than those in the MT group. The results of subgroup analysis indicated that patients with milder left ventricular dysfunction improved LVEF after AF ablation (mean difference: 6.53%, 95% CI: 6.18% to 6.88%; p<0.01) compared to patients with more severe disease (mean difference: 2.02%, 95% CI: 0.87% to 3.16%; p<0.01). Conclusions Our meta-analysis demonstrated that CA was associated with significant improvements in outcomes of AF patients with LVEF ≤45%. Additionally, AF patients with milder left ventricular dysfunction could benefit more from CA. (AF (HF coexist However (CA metaanalysis meta (MT (LVEF 45 ≤45% 116 2sided sided 2 2-sided pvalue p value 005 0 05 <0.0 allcause all cause ratio 052 52 0.52 95 CI 037 37 0.3 0.72 072 72 p<0.01 p001 01 mean difference 480 4 80 4.80% 229 29 2.29 7.31% 731 31 653 6 53 6.53% 618 18 6.18 6.88% 688 88 202 02 2.02% 087 87 0.87 3.16% 316 3 16 p<0.01. . Additionally ≤45 11 00 <0. 5 0.5 9 03 0. 0.7 07 p<0.0 p00 48 8 4.80 22 2.2 7.31 73 65 6.53 61 1 6.1 6.88 68 20 2.02 08 0.8 3.16 ≤4 <0 p<0. p0 4.8 2. 7.3 6.5 6. 6.8 2.0 3.1 ≤ < p<0 4. 7. 3. p<

Resumo Fundamento A fibrilação atrial (FA) e a insuficiência cardíaca (IC) coexistem frequentemente, resultando em desfechos adversos. No entanto, permanecem controvérsias quanto à eficácia da ablação por cateter (AC) em pacientes com FA com disfunção ventricular esquerda grave. Objetivos O objetivo deste estudo foi realizar uma metanálise de ensaios prospectivos randomizados e controlados para avaliar a eficácia da AC versus terapia médica (TM) em pacientes com FA com fração de ejeção do ventrículo esquerdo (FEVE) ≤45%. Métodos Procuramos na literatura estudos que comparassem AC com TM em pacientes com FA com FEVE ≤45%. Foi realizada uma metanálise de 7 ensaios clínicos, incluindo 1.163 pacientes com FA e IC. A análise de subgrupo foi realizada com base na FEVE basal. Todos os testes foram bilaterais; apenas o valor p <0,05 foi considerado estatisticamente significativo. Resultados Descobrimos que a AC estava associada a menor mortalidade por todas as causas (taxa de risco: 0,52, IC 95%: 0,37 a 0,72; p<0,01) e maiores melhorias na FEVE (diferença média: 4,80%, IC 95%: 2,29% a 7,31%; p<0,01) em comparação com TM. Os pacientes do grupo AC apresentaram menor risco de hospitalização por IC e recorrência de FA e qualidade de vida significativamente melhor do que aqueles do grupo TM. Os resultados da análise de subgrupo indicaram que pacientes com disfunção ventricular esquerda mais leve melhoraram a FEVE após a ablação de FA (diferença média: 6,53%, IC 95%: 6,18% a 6,88%; p<0,01) em comparação com pacientes com doença mais grave (diferença média : 2,02%, IC 95%: 0,87% a 3,16%; p<0,01). Conclusões Nossa metanálise demonstrou que a AC foi associada a melhorias significativas nos resultados de pacientes com FA com FEVE ≤45%. Além disso, pacientes com FA com disfunção ventricular esquerda mais leve poderiam se beneficiar mais com a AC. (FA (IC frequentemente adversos entanto (AC (TM (FEVE 45 ≤45% clínicos 1163 1 163 1.16 basal bilaterais 005 0 05 <0,0 significativo taxa 052 52 0,52 95% 95 037 37 0,3 0,72 072 72 p<0,01 p001 01 diferença 480 4 80 4,80% 229 2 29 2,29 7,31% 731 31 653 6 53 6,53% 618 18 6,18 6,88% 688 88 202 02 2,02% 087 87 0,87 3,16% 316 3 16 p<0,01. . disso ≤45 116 1.1 00 <0, 5 0,5 9 03 0, 0,7 07 p<0,0 p00 48 8 4,80 22 2,2 7,31 73 65 6,53 61 6,1 6,88 68 20 2,02 08 0,8 3,16 ≤4 11 1. <0 p<0, p0 4,8 2, 7,3 6,5 6, 6,8 2,0 3,1 ≤ < p<0 4, 7, 3, p<

RESUMEN Introducción: En los juegos de hockey sobre hielo, el rendimiento del equipo está influenciado por varios factores contextuales, y comprender los estilos de juego permite revelar cómo varían los indicadores clave de rendimiento en diferentes situaciones. Objetivo: Esta investigación tiene como objetivo explorar los estilos de juego de los equipos de hockey sobre hielo de élite e identificar aspectos clave del rendimiento en diferentes situaciones de diferencia de gol final. Métodos: El rendimiento del partido de 31 equipos de la Liga Nacional de Hockey durante 1271 partidos fue analizado y comparado, considerando el estilo de juego y la diferencia de gol final. Resultados: El análisis de componentes principales obtuvo 8 componentes de rendimiento que describen los estilos técnico-tácticos de los equipos. El análisis posterior encontró que hubo una diferencia significativa entre tres resultados de partido en estado desfavorable, penalizaciones principales, habilidad para mantener la posesión del disco, habilidad para defender el tiro, desempeño agresivo (p<0,001; = 0,007-0,273). Conclusión: Los equipos de clasificación más alta que ganaron los juegos desequilibrados mostraron un mejor rendimiento en la capacidad de defensa de disparos y en el rendimiento agresivo. Los equipos de clasificación más baja que perdieron en juegos desequilibrados mantuvieron menos posesión del disco y tenían más probabilidades de estar en desventaja numérica (p<0,05, ES=0,131-1,410). El estudio demuestra cómo los estilos de juego pueden utilizarse para contextualizar los determinantes clave de los juegos de hockey sobre hielo. Nivel de Evidencia I; Estudios Terapéuticos que Investigan los Resultados del Tratamiento. Introducción contextuales Objetivo final Métodos 3 127 comparado técnicotácticos técnico tácticos desfavorable tiro p<0,001 p0001 p 0 001 (p<0,001 0,0070,273. 00070273 0,007 0,273 . 007 273 0,007-0,273) Conclusión p<0,05, p005 05 (p<0,05 ES=0,1311,410. ES01311410 ES ES=0,131 1,410 131 1 410 ES=0,131-1,410) I Tratamiento 12 p<0,00 p000 00 (p<0,00 0070 0,0070,273 0007027 0007 0,00 0273 0,27 27 0,007-0,273 p<0,05 p00 (p<0,0 1311 ES=0,1311,410 ES0131141 ES0131 ES=0,13 1410 1,41 13 41 ES=0,131-1,410 p<0,0 0,0070,27 000702 000 0,0 027 0,2 2 0,007-0,27 p0 (p<0, ES=0,1311,41 ES013114 ES013 ES=0,1 141 1,4 4 ES=0,131-1,41 p<0, 0,0070,2 00070 0, 02 0,007-0,2 (p<0 ES=0,1311,4 ES01311 ES01 ES=0, 14 1, ES=0,131-1,4 p<0 0,0070, 0,007-0, (p< ES=0,1311, ES0 ES=0 ES=0,131-1, p< 0,0070 0,007-0 (p ES=0,1311 ES= ES=0,131-1 0,007- ES=0,131-

ABSTRACT Introduction: In ice hockey games, the team's performance is influenced by many contextual factors, and understanding playing styles allows to reveal how key performance indicators vary under different situations. Objective: This research aims to explore the playing styles of elite ice-hockey teams and to identify key performance aspects under different final goal difference situations. Methods: This article analyzed compared the match performance of 31 National Hockey League teams during 1271 matches considering their playing styles and final goal difference. Results: The principal component analysis obtained 8 performance components describing the technical-tactical styles of the teams. The subsequent analysis found that there was significant difference between three match outcomes in unfavorable state, major penalties, puck possession maintaining ability, shot defending ability, aggressive performance (p<0.001; = 0.007-0.273). Conclusions: Higher-ranked teams winning the unbalanced games showed better performance in shot defending ability and aggressive performance. Lower-ranked teams losing in unbalanced games kept less possession of the puck and were more likely to be shorthanded (p<0.05, ES=0.131-1.410). The study demonstrates how playing styles can be used to contextualize key determinants from ice hockey games. Level of evidence I; Therapeutic Studies Investigating the Results of Treatment. Introduction team s factors situations Objective icehockey Methods 3 127 technicaltactical technical tactical state penalties p<0.001 p0001 p 0 001 (p<0.001 0.0070.273. 00070273 0.007 0.273 . 007 273 0.007-0.273) Conclusions Higherranked Higher ranked Lowerranked Lower p<0.05, p005 05 (p<0.05 ES=0.1311.410. ES01311410 ES ES=0.131 1.410 131 1 410 ES=0.131-1.410) I Treatment 12 p<0.00 p000 00 (p<0.00 0070 0.0070.273 0007027 0007 0.00 0273 0.27 27 0.007-0.273 p<0.05 p00 (p<0.0 1311 ES=0.1311.410 ES0131141 ES0131 ES=0.13 1410 1.41 13 41 ES=0.131-1.410 p<0.0 0.0070.27 000702 000 0.0 027 0.2 2 0.007-0.27 p0 (p<0. ES=0.1311.41 ES013114 ES013 ES=0.1 141 1.4 4 ES=0.131-1.41 p<0. 0.0070.2 00070 0. 02 0.007-0.2 (p<0 ES=0.1311.4 ES01311 ES01 ES=0. 14 1. ES=0.131-1.4 p<0 0.0070. 0.007-0. (p< ES=0.1311. ES0 ES=0 ES=0.131-1. p< 0.0070 0.007-0 (p ES=0.1311 ES= ES=0.131-1 0.007- ES=0.131-

RESUMO Introdução: Nos jogos de hóquei no gelo, o desempenho da equipe é influenciado por vários fatores contextuais, e entender os estilos de jogo permite revelar como os principais indicadores de desempenho variam em diferentes situações. Objetivo: Esta pesquisa tem como objetivo explorar os estilos de jogo das equipes de hóquei no gelo de elite e identificar aspectos-chave de desempenho em diferentes estilos de jogo e a diferença do resultado final. Métodos: O desempenho de partida de 31 equipes da National Hockey League durante 1271 partidas foi analisado e comparado, considerando o estilo de jogo e a diferença de gol final. Resultados: A análise de componentes principais retornou 8 componentes de desempenho, descrevendo os estilos técnico-táticos das equipes. A análise subsequente revelou que houve diferença significativa entre três resultados de jogo em estado desfavorável, penalidades principais, habilidade de manter a posse do disco, habilidade de defender o lance e desempenho agressivo (p<0,001; = 0,007-0,273). Conclusão: As equipes de classificação mais alta que venceram os jogos em desequilíbrio numérico de jogadores apresentaram melhor desempenho na habilidade de defender o lance e no desempenho agressivo. As equipes de classificação mais baixa, que perderam em jogos desequilibrados, mantiveram menos posse do disco e tiveram maior probabilidade de ficar com um jogador a menos (p <0,05, ES = 0,131-1,410). O estudo demonstra como os estilos de jogo podem ser usados para contextualizar os principais determinantes dos jogos de hóquei no gelo. Nível de Evidência I; Estudos Terapêuticos Investigação dos Resultados do Tratamento. Introdução contextuais situações Objetivo aspectoschave aspectos chave final Métodos 3 127 comparado técnicotáticos técnico táticos desfavorável p<0,001 p0001 p 0 001 (p<0,001 0,0070,273. 00070273 0,007 0,273 . 007 273 0,007-0,273) Conclusão baixa desequilibrados 005 05 <0,05 0,1311,410. 01311410 0,131 1,410 131 1 410 0,131-1,410) I Tratamento 12 p<0,00 p000 00 (p<0,00 0070 0,0070,273 0007027 0007 0,00 0273 0,27 27 0,007-0,273 <0,0 1311 0,1311,410 0131141 0131 0,13 1410 1,41 13 41 0,131-1,410 p<0,0 p00 (p<0,0 0,0070,27 000702 000 0,0 027 0,2 2 0,007-0,27 <0, 0,1311,41 013114 013 0,1 141 1,4 4 0,131-1,41 p<0, p0 (p<0, 0,0070,2 00070 0, 02 0,007-0,2 <0 0,1311,4 01311 01 14 1, 0,131-1,4 p<0 (p<0 0,0070, 0,007-0, < 0,1311, 0,131-1, p< (p< 0,0070 0,007-0 0,1311 0,131-1 0,007- 0,131-

ABSTRACT Introduction: Atrial fibrillation is one of the common complications of mitral valve disease. Currently, in the absence of freezing equipment, it’s still impossible to fully conduct a minimally invasive Cox-maze IV procedure to treat atrial fibrillation. Methods: We analyzed the clinical data of 28 patients who underwent thoracoscopic minimally invasive mitral valve full maze surgery in our hospital from October 2021 to September 2022; 13 patients were male and 15 were female, three suffered from paroxysmal atrial fibrillation, and 25 suffered from permanent atrial fibrillation; average age was 61.88±8.30 years, and mean preoperative left atrial diameter was 47.12±8.34 mm. Isolation of left atrial posterior wall (box lesion) was completed in all patients by cut-and-sew technique and bipolar clamp ablation. Results: For these subjects, the median cardiopulmonary bypass time was 169 (109.75-202.75) minutes, aortic cross-clamping time was 106 (77.75-125.50) minutes, and ventilator assistance time was 6.5 (0-10) hours. Among them, eight subjects had the endotracheal tubes removed immediately after surgical operation. Three subjects were in the blanking period; two subjects still had atrial fibrillation at three months after operation, one of whom resumed sinus rhythm after electrical cardioversion therapy; and all the remaining 23 subjects had sinus rhythm. Conclusion: The minimally invasive cut-and-sew technique for electrical isolation of left pulmonary veins can improve sinus conversion rate of patients suffering from both mitral valve disease and atrial fibrillation. In selected subjects, it is safe and has good results in the short-term postoperative period. Introduction Currently equipment its s Coxmaze Cox Methods 2 202 2022 1 female 6188830 61 88 8 30 61.88±8.3 years 4712834 47 12 34 47.12±8.3 mm box lesion cutandsew cut sew ablation Results 16 109.75202.75 1097520275 109.75 202.75 109 75 (109.75-202.75 minutes crossclamping cross clamping 10 77.75125.50 777512550 77.75 125.50 77 125 50 (77.75-125.50 65 6 5 6. 010 0 (0-10 hours them operation period therapy Conclusion shortterm short term 20 618883 3 61.88±8. 471283 4 47.12±8. 75202 109.75202.7 109752027 10975 109.7 20275 202.7 7 (109.75-202.7 75125 77.75125.5 77751255 7775 77.7 12550 125.5 (77.75-125.5 01 (0-1 61888 61.88±8 47128 47.12±8 7520 109.75202. 10975202 1097 109. 2027 202. (109.75-202. 7512 77.75125. 7775125 777 77. 1255 125. (77.75-125. (0- 6188 61.88± 4712 47.12± 752 109.75202 1097520 (109.75-202 751 77.75125 777512 (77.75-125 (0 618 61.88 471 47.12 109.7520 109752 (109.75-20 77.7512 77751 (77.75-12 ( 61.8 47.1 109.752 (109.75-2 77.751 (77.75-1 61. 47. (109.75- (77.75- (109.75 (77.75 (109.7 (77.7 (109. (77. (109 (77 (10 (7 (1

Coenzyme Q10 (CoQ10) is a potent antioxidant that is implicated in the inhibition of osteoclastogenesis, but the underlying mechanism has not been determined. We explored the underlying molecular mechanisms involved in this process. RAW264.7 cells received receptor activator of NF-κB ligand (RANKL) and CoQ10, after which the differentiation and viability of osteoclasts were assessed. After the cells were treated with CoQ10 and/or H2O2 and RANKL, the levels of reactive oxygen species (ROS) and proteins involved in the PI3K/AKT/mTOR and MAPK pathways and autophagy were tested. Moreover, after the cells were pretreated with or without inhibitors of the two pathways or with the mitophagy agonist, the levels of autophagy-related proteins and osteoclast markers were measured. CoQ10 significantly decreased the number of TRAP-positive cells and the level of ROS but had no significant impact on cell viability. The relative phosphorylation levels of PI3K, AKT, mTOR, ERK, and p38 were significantly reduced, but the levels of FOXO3/LC3/Beclin1 were significantly augmented. Moreover, the levels of FOXO3/LC3/Beclin1 were significantly increased by the inhibitors and mitophagy agonist, while the levels of osteoclast markers showed the opposite results. Our data showed that CoQ10 prevented RANKL-induced osteoclastogenesis by promoting autophagy via inactivation of the PI3K/AKT/mTOR and MAPK pathways in RAW264.7 cells. Q Q1 CoQ (CoQ10 determined process RAW2647 RAW RAW264 7 RAW264. NFκB NF κB RANKL (RANKL assessed CoQ1 andor HO H O H2O (ROS PI3KAKTmTOR PIKAKTmTOR PI3K AKT mTOR PI K tested Moreover agonist autophagyrelated related measured TRAPpositive TRAP positive PIK ERK p p3 reduced FOXO3LC3Beclin1 FOXOLCBeclin FOXO3 LC3 Beclin1 FOXO LC Beclin FOXO3/LC3/Beclin augmented results RANKLinduced induced (CoQ1 RAW26 KAKTmTOR FOXO3LC3Beclin (CoQ RAW2

Abstract Objective This retrospective study aimed to analyze the clinical efficacy of two regenerative surgical methods — Bio-Oss granules combined with barrier membranes and Bio-Oss Collagen alone — and to help clinicians achieve better periodontal regeneration outcomes in the specific periodontal condition. Methodology Patients who underwent periodontal regeneration surgery from January 2018 to April 2022 were retrospectively screened, and their clinical and radiographic outcomes at 6 months postoperatively were analyzed. The probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival recession (GR), distance from the cemento-enamel junction to the bottom of the bone defect (CEJ-BD), and depth of intrabony defects (INFRA) were recorded before the operation (T0) and 6 months after it (T1), and subsequently compared. Results In total, 143 patients were included — 77 were placed in the Bio-Oss group and 66 were placed in the Bio-Oss Collagen group. All indicators, including PD and CAL at T1, showed significant differences compared to baseline, for both groups (P<0.001). PD reduction was greater in the group receiving the Bio-Oss Collagen treatment (P=0.042). Furthermore, in cases when the baseline PD range was 7-11 mm and the age range was 35-50 years, PD reduction was more significant for patients receiving the Bio-Oss Collagen treatment (P=0.031, 0.023). A linear regression analysis indicated that postoperative PD and CAL were positively correlated with baseline values, and that the efficacy tended to decrease with increasing age. Conclusion Both the use of Bio-Oss Collagen alone and the use of Bio-Oss granules combined with barrier membranes resulted in significant effects in the treatment of periodontal intrabony defects. The Bio-Oss Collagen treatment generated more improvements in PD than the Bio-Oss granules combined with barrier membranes, particularly within the baseline PD range of 7-11 mm and the 35-50 years age group. Additionally, age was the main factor influencing the effectiveness of regenerative surgery for intrabony defects: older individuals exhibited fewer improvements. BioOss Bio Oss condition 201 202 screened analyzed PD, , (PD) CAL, (CAL) BOP, BOP (BOP) GR, GR (GR) cementoenamel cemento enamel CEJBD, CEJBD CEJ BD (CEJ-BD) INFRA (INFRA T0 T (T0 T1 (T1) total 14 7 indicators P<0.001. P0001 P P<0.001 . 0 001 (P<0.001) P=0.042. P0042 P=0.042 042 (P=0.042) Furthermore 711 11 7-1 3550 35 50 35-5 P=0.031, P0031 031 (P=0.031 0.023. 0023 0.023 023 0.023) values Additionally 20 (PD (CAL (BOP (GR (CEJ-BD (T (T1 1 P000 P<0.00 00 (P<0.001 P004 P=0.04 04 (P=0.042 71 7- 355 3 5 35- P=0.031 P003 03 (P=0.03 002 0.02 02 2 P00 P<0.0 (P<0.00 P=0.0 (P=0.04 P=0.03 (P=0.0 0.0 P0 P<0. (P<0.0 P=0. (P=0. 0. P<0 (P<0. P=0 (P=0 P< (P<0 P= (P= (P< (P

Abstract Aims Very Low Birthweight (VLBW) infants with neonatal Chronic Lung Disease (CLD) have been found to have functional impairment of the brainstem auditory pathway at term. This study investigated the functional status of the brainstem auditory pathway in VLBW infants with CLD after term for any abnormality. Methods Fifty-two VLBW infants were recruited at 50 weeks of Postconceptional Age: 25 with neonatal CLD and 27 without CLD. None had any other major complications to minimize confounding effects. Brainstem Auditory Evoked Responses were studied at 21‒91/s click rates. Results Compared with those without CLD, VLBW infants with CLD had relatively shorter latencies of BAER waves I and III, associated with a slightly lower BAER threshold. Wave V latency and I‒V interpeak interval did not differ significantly between the two groups of infants. The I‒III interval in infants with CLD was shorter than in those without CLD at 91/s clicks. However, the III‒V interval was significantly longer than in those without CLD at all click rates (all p < 0.05). There were no significant differences in the amplitudes of BAER wave components between the two groups of infants. Conclusions The main BAER abnormality in VLBW infants with CLD was a prolonged III‒V interval. Auditory conduction is delayed or impaired at more central regions of the brainstem in CLD infants. After term central auditory function is adversely affected by neonatal CLD. Monitoring post-term change is required to provide valuable information for post-term care of CLD infants. (VLBW (CLD Fiftytwo Fifty 5 Age 2 effects 2191s s 21 91 III threshold IV IIII 91s clicks However IIIV 0.05. 005 0.05 . 0 05 0.05) postterm post 9 00 0.0 0.

Abstract Purpose Word-of-mouth (WOM) valence refers to the favorable impact of positive WOM on marketing outcomes. However, studies have shown that negative WOM can have a greater influence. Therefore, this study aims to explore the impact of negative online WOM (OWOM) on marketing. Theoretical framework Drawing from the perspectives of psychology and communication, and using the sender-message-channel-receiver (SMCR) communication process theory, we construct a moderated mediation model to explore the mechanism and threshold of WOM recipients’ product involvement in reversing the impact of WOM valence on product attitude. Design/methodology/approach SPSS 22.0 and SmartPLS 3.0 were used to empirically analyze the survey data from a sample of 1,107 consumers. Findings Our study reveals a nonlinear relationship between negative OWOM recipients’ product involvement and consumer product attitude, mediated by risk perception and moderated by sender characteristics and WOM characteristics. The relationship shows a U-shaped curve between product involvement and product attitude, and an inverted U-shaped curve between product involvement and risk perception. Risk perception mediates the relationship between product involvement and product attitude, which varies with the strength of the sender-recipient relationship and the amount of negative WOM. Sender professionalism moderates both the U-shaped and the inverted U-shaped curves. Practical & social implications of research From a practical standpoint, our findings have implications for the management of OWOM marketing for fresh agricultural products. Originality/value Our study provides a more nuanced understanding of the impact of negative WOM on consumer attitudes, challenging the one-sided focus on strengthening positive WOM valence in the context of the Internet. Wordofmouth Word mouth (WOM outcomes However influence Therefore (OWOM sendermessagechannelreceiver message channel receiver SMCR (SMCR theory recipients attitude Designmethodologyapproach Design methodology approach 220 22 0 22. 30 3 3. 1107 1 107 1,10 consumers Ushaped U shaped senderrecipient recipient curves standpoint products Originalityvalue Originality value attitudes onesided one sided Internet 2 110 10 1,1 11 1,

ABSTRACT Purpose: Pre-eclampsia (PE) is a pregnancy-related complication. Eucommia is effective in the treatment of hypertensive disorders in pregnancy, but the specific effects and possible mechanisms of Eucommia granules (EG) in PE remain unknown. The aim of this study was to investigate the effects and possible mechanisms of EG in PE rats. Methods: Pregnant Sprague Dawley rats were divided into five groups (n = 6): the control group, the model group, the low-dose group, the medium-dose group, and the high-dose group of EG. The PE model was established by subcutaneous injection of levonitroarginine methyl ester. Saline was given to the blank and model groups, and the Eucommia granules were given by gavage to the remaining groups. Blood pressure and urinary protein were detected. The body length and weight of the pups and the weight of the placenta were recorded. Superoxide dismutase (SOD) activity and levels of malondialdehyde (MDA), placental growth factor (PIGF), and soluble vascular endothelial growth factor receptor-1 (sFIt-1) were measured in the placenta. Pathological changes were observed by hematoxylin-eosin staining. Wnt/β-catenin pathway-related protein expression was detected using Western blot. Results: Compared with the model group, the PE rats treated with EG had lower blood pressure and urinary protein. The length and weight of the pups and placental weight were increased. Inflammation and necrosis in the placental tissue was improved. SOD level increased, MDA content and sFIt-1/PIGF ratio decreased, and Wnt/β-catenin pathway-related protein expression level increased. Moreover, the results of EG on PE rats increased with higher doses of EG. Conclusions: EG may activate the Wnt/β-catenin pathway and inhibit oxidative stress, inflammation, and vascular endothelial injury in PE rats, thereby improving the perinatal prognosis of preeclamptic rats. EG may inhibit oxidative stress, inflammation, and vascular endothelial injury through activation of the Wnt/β-catenin pathway in preeclampsia rats, thereby improving perinatal outcomes in PE rats. Purpose Preeclampsia Pre eclampsia (PE pregnancyrelated pregnancy related complication (EG unknown Methods n 6 6) lowdose low dose mediumdose medium highdose high ester recorded (SOD MDA, , (MDA) PIGF, PIGF (PIGF) receptor1 receptor 1 receptor- sFIt1 sFIt (sFIt-1 hematoxylineosin hematoxylin eosin staining Wnt/βcatenin Wntβcatenin Wnt/β catenin Wnt β pathwayrelated blot Results improved sFIt1/PIGF sFIt1PIGF sFItPIGF 1/PIGF decreased Moreover Conclusions stress inflammation (MDA (PIGF (sFIt- βcatenin Wntβ 1PIGF (sFIt

Abstract Background Cutaneous squamous cell carcinoma (cSCC) develops from epithelial keratinocytes by dysregulation of self-renewal and differentiation. Recent studies have found that the size and number of cSCC tumors gradually decrease or even disappear after HPV vaccination. However, the role of the HPV vaccine in the cSCC mechanism is poorly understood. Objective The aim of this study is to investigate the effect and mechanism of the HPV vaccine in cSCC. Methods Immunofluorescence was used to study the immune infiltrating cells in the tumor tissues of patients with cSCC. The effects of the HPV vaccine on cSCC cells and tissues were studied by Cell Culture, Real-time PCR, Western Blot, Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay, m6A Blotting, CCK-8 Assay, m6A Ribonucleic acid Methylation Quantification and tumor transplantation. Results The HPV vaccine enhanced the toxic effect of CD8+T cells on cSCC cells and promoted the secretion of multiple cytokines by CD8+T cells. In addition, HPV vaccines can increase tumor sensitivity to anti-PD-1 therapy by downregulating METTL3 in tumor tissue, with the combination of HPV vaccine and PD-1 monoclonal antibodies producing enhanced immune cell infiltration compared to PD-1 blockade alone. Study limitations It is important to note the limitations of this study, including the small sample size, the construction of the mouse model, and the choice of HPV vaccine and PD-1 monoclonal antibody, which may limit the generalization of our findings to a wider population. Conclusions It is hoped that this research will contribute to a deeper understanding of the role of the HPV vaccine in the treatment of cSCC. HPV vaccine is expected to become an important approach to alleviate the development of cSCC. (cSCC selfrenewal self renewal differentiation vaccination However understood Culture Realtime Real time PCR Blot Assay Enzymelinked Enzyme linked mA m A Blotting CCK8 CCK 8 CCK- transplantation CD8T CDT CD8 T CD addition antiPD1 antiPD anti PD 1 anti-PD- METTL tissue PD1 PD- alone model antibody population anti-PD

Abstract The sea cucumber beer was successfully brewed by the optimal dosage of sea cucumber enzymatic hydrolysate (10%) and pilot-scale brewing technology were determined, then unique volatile compounds and anticoagulant activity of sea cucumber beer were analyzed in this paper. The brewing results of sea cucumber beer showed that foam stability (244 ± 5 s), original gravity (14.03 ± 0.06 oP), alcohol level (5.90 ± 0.10 %, vol), residual sugar content (2.00 ± 0.17 g/L) and total acidity (2.40 ± 0.1 mL/100mL) were significantly lower than that of control beer, but the color (17.00 ± 0.10 EBC) and pH (4.97 ± 0.06) were obviously higher than that of the control, and 30 kinds of unique volatile compounds (nonanol, 1-decanol, nonanal, phenylethyl acetate, 6-methyl-5-hepten-2-one, etc) were identified in the final sea cucumber beer, and which were not existent in the control beer. In addition, the anticoagulant activity of sea cucumber beer was assessed by measuring the values of Activated Partial Thromboplatin Time (APTT, 127.83 ± 1.10 s), Prothrombin Time (PT, 30. 08 ± 0.40 s), and Thrombin Time (TT, 26.69 ± 1.37 s), which were higher than those of control beer and will benefit to prophylaxis and treatment of thrombosis for people. 10% 10 (10% pilotscale pilot scale determined paper 244 (24 s, s , s) 14.03 1403 14 03 (14.0 006 0 06 0.0 oP, oP oP) 5.90 590 90 (5.9 010 % vol, vol vol) 2.00 200 2 00 (2.0 017 17 g/L gL g L 2.40 240 40 (2.4 01 1 0. mL/100mL mL100mL mLmL mL 100mL 17.00 1700 (17.0 EBC 4.97 497 4 97 (4.9 3 nonanol, nonanol (nonanol 1decanol, 1decanol decanol decanol, 1-decanol nonanal acetate 6methyl5hepten2one, 6methyl5hepten2one methylheptenone 6 methyl hepten one, one 6-methyl-5-hepten-2-one etc addition APTT, APTT (APTT 12783 127 83 127.8 110 1.1 PT, PT (PT 040 0.4 TT, TT (TT 2669 26 69 26.6 137 37 1.3 people (10 24 (2 14.0 140 (14. 5.9 59 9 (5. 2.0 20 (2. 2.4 17.0 170 (17. 4.9 49 (4. 1278 12 8 127. 11 1. 04 266 26. 13 (1 ( 14. (14 5. (5 2. 17. (17 4. (4

Abstract Background Abnormal motor posturing (AMP), exhibiting as decorticate, decerebrate, or opisthotonos, is regularly noticed among children and adults. Objective This systematic review and meta-analysis examined the risk factors and outcome of posturing among severe head and brain injury subjects. Methods Based on the inclusion and exclusion criteria and using MeSH terms: "decerebrate posturing", "opisthotonic posturing", "brain injury", and/or "cerebral injury" articles were searched on Scopus, PubMed, Science Direct, and google scholar databases. Observational studies, case series, and case reports were included. Results A total of 1953 studies were retrieved initially, and based on the selection criteria, 20 studies were finally selected for review and were analyzed for meta-analysis based on the mortality between the hematomas. The functional outcomes of this study are the risk factors, mortality rate and Glasgow Outcome Scale. Decerebrative patients were higher among the studies related to head injury surgeries. Males were mainly treated for decerebrate postures compared with the female subjects. Extradural hematoma and acute subdural hematoma with cerebral contusion were quite common in the surgical mass lesions. Conclusion The findings reported that the lesion types, the operative procedures, and the age of the decerebrating patients with brain injuries are the significant prognostic factors determining the survival outcomes. AMP, AMP , (AMP) decorticate opisthotonos adults metaanalysis meta analysis subjects terms posturing, posturing" opisthotonic injury, andor Scopus PubMed Direct databases series included 195 initially 2 hematomas Scale surgeries lesions types procedures (AMP 19 1

Resumo Antecedentes Postura motora anormal (AMP), exibindo-se como decorticada, descerebrada ou opistótono, é regularmente observada entre crianças e adultos. Objetivo Esta revisão sistemática e metanálise examinou os fatores de risco e os resultados da postura entre indivíduos com lesões graves na cabeça e no cérebro. Métodos Com base nos critérios de inclusão e exclusão e usando termos MeSH: artigos sobre "postura descerebrada", "postura opistótona", "lesão cerebral" e/ou "lesão cerebral" foram pesquisados nas bases de dados Scopus, PubMed, Science Direct e Google Scholar. Foram incluídos estudos observacionais, séries de casos e relatos de casos. Resultados Um total de 1.953 estudos foram recuperados inicialmente e, com base nos critérios de seleção, 20 estudos foram finalmente selecionados para revisão e analisados para metanálise com base na mortalidade entre os hematomas. Os resultados funcionais deste estudo são os fatores de risco, taxa de mortalidade e Escala de Resultados de Glasgow. Os pacientes descerebrados foram maiores entre os estudos relacionados a cirurgias de traumatismo cranioencefálico. Os homens foram tratados principalmente para posturas descerebradas em comparação com as mulheres. Hematoma extradural e hematoma subdural agudo com contusão cerebral foram bastante comuns nas lesões de massa cirúrgica. Conclusão Os achados relataram que os tipos de lesões, os procedimentos operatórios e a idade dos pacientes descerebrados com lesões cerebrais são os fatores prognósticos significativos que determinam os resultados de sobrevivência. AMP, AMP , (AMP) exibindose exibindo se decorticada opistótono adultos cérebro MeSH descerebrada, descerebrada" opistótona, opistótona opistótona" lesão eou Scopus PubMed Scholar observacionais 1953 1 953 1.95 seleção 2 hematomas Glasgow cranioencefálico mulheres cirúrgica sobrevivência (AMP 195 95 1.9 19 9 1.

The modification of magnesium alloy through the addition of rare-earth elements improves specific properties of magnesium alloy. However, the evolution mechanisms of rare-earth precipitates during thermal cycles are still unclear, particularly during the welding thermal cycles. Therefore, deep research on the influence mechanisms of rare-earth precipitates on the welded joint is necessary. In this paper, Ce-containing Mg alloy was successfully welded by laser welding. The microstructure of the welded joint was deeply analyzed. As a result, the elemental composition and distribution rules of precipitates within the fusion zone were obtained. In addition, heat treatment was carried out for the welded joint, thus deducing the evolution mechanisms of precipitates within the joint. The corresponding results revealed that typical micron/sub-micron-sized particles, consisting of both conventional and rare-earth intermetallics, are formed and neatly distributed within the fusion zone, demonstrating that the rare-earth particles inside the fusion zone are much smaller in size than that of Mg17Al12. Tensile test results suggest that these tiny particles significantly improved the strength of the fusion zone, promoting the tensile-shear strength of the joint accounting for 76.6% of that of the base metal. The above approach provides a feasible solution for the welding of rare-earth Mg alloys. rareearth rare earth However unclear Therefore necessary paper Cecontaining Ce containing analyzed result obtained micron/submicronsized micronsubmicronsized micron/sub micron sized sub intermetallics Mg17Al12 MgAl Al tensileshear tensile shear 766 76 6 76.6 metal alloys submicronsized micronsub Mg17Al1 7 76. Mg17Al

Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains the leading cause of mortality by a single infectious agent in the world. M. tuberculosis infection could also result in clinical chronic infection, known as latent TB infection (LTBI). Compared to the current limited treatment, several subunit vaccines showed immunotherapeutic effects and were included in clinical trials. In this study, a subunit vaccine of Ag85B with a novel mucosal adjuvant c-di-AMP (Ag85B:c-di-AMP) was delivered intranasally to a persistent M. tuberculosis H37Ra infection mouse model, which also presented the asymptomatic characteristics of LTBI. Compared with Ag85B immunization, Ag85B:c-di-AMP vaccination induced stronger humoral immune responses, significantly higher CD4+ T cells recruitment, enhanced Th1/Th2/Th17 profile response in the lung, decreased pathological lesions of the lung, and reduced M. tuberculosis load in mice. Taken together, Ag85B:c-di-AMP mucosal route immunization provided an immunotherapeutic effect on persistent M. tuberculosis H37Ra infection, and c-di-AMP, as a promising potential mucosal adjuvant, could be further used in therapeutic or prophylactic vaccine strategies for persistent M. tuberculosis infection as well as LTBI. TB, , (TB) world M LTBI . (LTBI) treatment trials study AgB Ag B cdiAMP c di AMP Ag85BcdiAMP AgBcdiAMP (Ag85B:c-di-AMP HRa H Ra model responses CD4 CD recruitment Th1Th2Th17 ThThTh Th1 Th2 Th17 Th Th1/Th2/Th1 lung mice together cdiAMP, AMP, (TB (LTBI BcdiAMP Th1Th2Th1 Th1/Th2/Th Th1Th2Th