Este estudio exploró las perspectivas de los profesionales de la salud sobre la presencia familiar durante la atención de un paro cardiopulmonar (PCA) en adultos. Se empleó un diseño observacional transversal, siguiendo las pautas de Fortalecimiento de la Notificación de Estudios Observacionales en Epidemiología (STROBE). Los datos fueron recolectados a través de un formulario electrónico utilizando un método de muestreo no probabilístico, resultando en 221 formularios completados para su análisis. Se encontraron asociaciones significativas entre las perspectivas de los participantes sobre la presencia familiar y sus características demográficas. Los profesionales mayores de 40 años demostraron una mayor prevalencia de aceptación de la presencia familiar (p lt; 0,01). Por el contrario, los profesionales más jóvenes (menores de 40 años) expresaron su preocupación por el posible impacto negativo en la prestación de atención (p = 0,02). La experiencia con presencia familiar fue reportada con mayor frecuencia entre aquellos con 10 o menos años de experiencia profesional (p lt;0,01). Además, los profesionales con más de 20 años de experiencia tenían más probabilidades de creer que la presencia familiar podría alterar la concentración del personal (p = 0,02). Los hallazgos sugieren una tendencia entre los participantes a ver la presencia familiar de manera negativa, a pesar de su potencial para ayudar a las familias a afrontar la pérdida y el dolor. Esta cuestión merece una mayor investigación y discusión.

This study explored healthcare professionals’ perspectives on family presence during adult cardiopulmonary arrest (CPA) care. An observational, cross-sectional design was employed, adhering to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. Data were collected through an electronic form using a nonprobabilistic sampling method, resulting in 221 completed forms for analysis. Significant associations were found between participants’ perspectives on family presence and their demographic characteristics. Professionals aged over 40 demonstrated a higher prevalence of accepting family presence (p lt; 0.01). Conversely, younger professionals (under 40) expressed concerns about the potential negative impact on care delivery (p = 0.02). Experience with family presence was more commonly reported among those with 10 or fewer years of professional experience (p lt; 0.01). Additionally, professionals with over 20 years of experience were more likely to believe that family presence could disrupt staff concentration (p = 0.02). The findings suggest a tendency among participants to view family presence negatively, despite its potential to help families cope with loss and grief. This issue warrants further investigation and discussion.

Este estudo explorou as perspectivas dos profissionais de saúde sobre a presença da família durante o atendimento à parada cardiorrespiratória (PCR) em adultos. Foi empregado um desenho observacional e transversal, seguindo as diretrizes do Strengthening the Reporting of Observational Studies in Epidemiology (STROBE). Os dados foram coletados por meio de formulário eletrônico, utilizando método de amostragem não probabilística, resultando em 221 formulários preenchidos para análise. Foram encontradas associações significativas entre as perspectivas dos participantes sobre a presença familiar e suas características demográficas. Profissionais com idade superior a 40 anos demonstraram maior prevalência de aceitação da presença familiar (p lt; 0,01). Por outro lado, os profissionais mais jovens (menos de 40 anos) expressaram preocupações sobre o potencial impacto negativo na prestação de cuidados (p = 0,02). A experiência com a presença familiar foi mais relatada entre aqueles com 10 ou menos anos de experiência profissional (p lt; 0,01). Além disso, profissionais com mais de 20 anos de experiência tiveram maior probabilidade de acreditar que a presença da família poderia atrapalhar a concentração da equipe (p = 0,02). Os resultados sugerem uma tendência entre os participantes de verem a presença familiar de forma negativa, apesar do seu potencial para ajudar as famílias a lidar com a perda e o luto. Esta questão merece mais investigação e discussão.

Abstract One of the crucial aspects to be considered for successful in vitro production (IVP) of embryos is the composition of the various media used throughout the stages of this reproductive biotechnology. The cell culture media employed should fulfill the metabolic requirements of both gametes during oocyte maturation and sperm development, as well as the embryo during its initial cell divisions. Most IVP protocols incorporate blood serum into the media composition as a source of hormones, proteins, growth factors, and nutrients. Numerous studies have suggested Platelet-Rich Plasma (PRP) as a substitute for fetal sera in cell culture, particularly for stem cells. Therefore, the objective of this study is to assess the potential use of PRP as a replacement for fetal bovine serum (FBS) during oocyte maturation for in vitro production of bovine embryos. During in vitro maturation (IVM), cumulus-oocyte complexes (COCs) were allocated into the following experimental groups: Group G1 (IVM medium with 5% PRP); Group G2 (MIV medium with 5% PRP and 5% SFB); Group G3 (MIV medium with 5% SFB); and Group G4 (MIV medium without either PRP or SFB). Subsequently, the cumulus-oocyte complexes were fertilized with semen from a single bull, and the resulting zygotes were cultured for seven days. Cleavage and blastocyst formation rates were assessed on days 2 and 7 of embryonic development, respectively. The quality of matured COCs was also evaluated by analyzing the gene expression of HSP70, an important protein associated with cellular stress. The results demonstrated that there were no significant differences among the experimental groups in terms of embryo production rates, both in the initial cleavage stages and blastocyst formation (except for the G4 group, which exhibited a lower blastocyst formation rate on D7, as expected). This indicates that PRP could be a cost-effective alternative to SFB in the IVP of embryos. (IVP biotechnology development divisions hormones proteins factors nutrients PlateletRich Platelet Rich (PRP cells Therefore FBS (FBS IVM, IVM , (IVM) cumulusoocyte cumulus (COCs G 5 PRP) MIV SFB) SFB. . Subsequently bull respectively HSP70 HSP stress except group D7 D expected. expected expected) costeffective cost effective HSP7

ABSTRACT Background: Although canine visceral leishmaniasis (CVL) transmission primarily occurs through the bite of phlebotomine sand flies infected with Leishmania infantum, alternative routes may exist. Methods: Thirty-four dogs diagnosed with CVL were sampled for parasitological investigation in tissues from the reproductive tract. Results: Amastigotes of Leishmania sp. were present in 79% (27/34) of the reproductive system samples, with distinct infection rates depending on the tissue. Conclusions: Our data confirms that alternative routes, such as horizontal and vertical transmissions, should be considered in the epidemiological chain of CVL. Background (CVL infantum exist Methods Thirtyfour Thirty four tract Results sp 79 27/34 2734 27 34 (27/34 samples tissue Conclusions transmissions 7 27/3 273 2 3 (27/3 27/ (27/ (27 (2 (

Abstract: This review aimed to identify and synthesize the perceptions of mental healthcare professionals, family members, and users about mental health crisis interventions for children and adolescents at hospitals and community mental health services. A qualitative systematic review was conducted following the Joanna Briggs Institution guidelines. The search was performed in 15 databases, with no temporal delimitation, and included studies in Portuguese, English, and Spanish. All works were assessed regarding methodological quality, credibility, and dependability according to the ConQual score and the recommendations were assessed following the Joanna Briggs Institution guidelines. In total, two independent reviewers screened and assessed the studies, extracted their data, developed categories, and conducted the thematic synthesis. A total of 13 studies met the inclusion and exclusion criteria. From these, five syntheses were developed: importance of relationships; importance of procedures during treatment; positive emotional responses to treatment; negative emotional responses to treatment; and issues with health professionals and health services. All five syntheses presented high dependability; two syntheses presented high credibility; and three presented moderate credibility. Mental healthcare professionals, family members and users had convergent perceptions about crisis intervention provided at healthcare services. Understanding their perceptions to improve care and the user experience in this vulnerable situation is crucial.

Resumo: Esta revisão teve como objetivo identificar e sintetizar as percepções de profissionais de saúde mental, familiares e usuários sobre a intervenção em crises de saúde mental de crianças e adolescentes em hospitais e serviços comunitários especializados. Uma revisão sistemática qualitativa foi conduzida de acordo com as diretrizes do Instituto Joanna Briggs. A busca foi feita em 15 bases de dados, sem delimitação temporal, selecionando estudos nos idiomas português, inglês e espanhol. Todos os estudos incluídos foram avaliados quanto à qualidade metodológica, credibilidade e confiabilidade de acordo com o escore ConQual e as recomendações foram avaliadas pelas diretrizes do Instituto Joanna Briggs. Dois revisores independentes examinaram e avaliaram os estudos, extraíram dados, e elaboraram categorias e síntese temática. Ao todo, 13 estudos atenderam aos critérios de inclusão e exclusão. Foram elaboradas cinco sínteses: importância dos relacionamentos; importância dos procedimentos durante o tratamento; respostas emocionais positivas ao tratamento; respostas emocionais negativas ao tratamento; e questões sobre a equipe de saúde e os serviços de saúde. Todas as cinco sínteses apresentaram alta confiabilidade; duas sínteses apresentaram alta credibilidade; e três apresentaram credibilidade moderada. Profissionais de saúde mental, familiares e usuários apresentaram percepções convergentes sobre a intervenção em crises prestada nos serviços de saúde. É fundamental entender as percepções do usuário para melhorar seu atendimento e experiência nessa situação vulnerável.

Resumen: Esta revisión tuvo como objetivo identificar y sintetizar las percepciones de profesionales de salud mental, familiares y usuarios sobre la intervención en crisis de salud mental de niños y adolescentes en hospitales y servicios comunitarios especializados. Se realizó una revisión sistemática cualitativa de acuerdo con las directrices del Instituto Joanna Briggs. La búsqueda se realizó en 15 bases de datos, sin delimitación temporal, seleccionando estudios en portugués, inglés y español. Todos los estudios incluidos se evaluaron en cuanto a la calidad metodológica, la credibilidad y la confiabilidad de acuerdo con la puntuación ConQual y las recomendaciones se evaluaron utilizando las directrices del Instituto Joanna Briggs. Dos revisores independientes examinaron y evaluaron los estudios, extrajeron datos y elaboraron categorías y síntesis temáticas. En total, 13 estudios cumplieron con los criterios de inclusión y exclusión. Se elaboraron cinco síntesis: importancia de las relaciones; importancia de los procedimientos durante el tratamiento; respuestas emocionales positivas al tratamiento; respuestas emocionales negativas al tratamiento; y preguntas sobre el equipo de salud y los servicios de salud. Todas las cinco síntesis mostraron una alta confiabilidad; dos síntesis presentaron alta credibilidad; y tres presentaron una credibilidad moderada. Profesionales de salud mental, familiares y usuarios presentaron percepciones convergentes sobre la intervención en crisis brindada en los servicios de salud. Es fundamental comprender las percepciones de los usuarios para mejorar su atención y experiencia en esta situación vulnerable.

Abstract Malignant neoplasms are increasingly prevalent in the daily clinical practice. Up to 61% of patients with pelvic malignancies undergo pelvic radiotherapy in different doses, which may cause intestinal damage, and the rectum is the segment most frequently affected due to its fixed position in the pelvis. Currently, there are several strategies to minimize the effects of radiation on the tissues surrounding the neoplastic site; despite those strategies, radiotherapy can still result in serious damage to organs and structures, and these injuries accompany patients throughout their lives. One of the most common damages resulting from pelvic radiotherapy is acute proctitis.The diagnosis is confirmed by visualizing the rectal mucosa through rigid or flexible rectosigmoidoscopy and colonoscopy. The objective of the present study was to review the forms of radiation-induced proctopathytherapy, and to evaluate the results of each method to propose a standardization for the treatment of this pathology. Despite the prevalence of radiation-induced proctopathy, there is no definitive standardized treatment strategy so far. The first approach can be tried with local agents, such as mesalazine and formalin. For refractory cases, control can usually be achieved with argon plasma coagulation, hyperbaric oxygen, and radiofrequency ablation therapies. Regarding the study of radiation-induced proctopathy, there is a lack of robust studies with large samples and standardized therapies to be compared. There is a lack of double-blinded, randomized controlled studies to determine a definitive standard treatment algorithm.

BACKGROUND Iron homeostasis contribute for the human immunodeficiency virus (HIV) pathogenesis. OBJECTIVES We assessed the iron intake pattern in antiretroviral naïve Brazilian men living with HIV correlating with clinical and nutritional parameters. METHODS The iron consumption mean was estimated according to a food frequency questionnaire (FFQ), and a 3-day food record (3dFR) submitted to the patients. HIV viral load, CD4+ T cell counts, serum iron, haematological and anthropometrics parameters were recorded. FINDINGS Fifty-one HIV-infected adult men naïve for antiretroviral therapy (ART) were enrolled. The mean age of participants was 35 (SEM ± 1.28) years old, with mean time of HIV-1 infection of 1.78 (0-16.36, min-max) years. Majority (41.18%) had complete secondary, and 21.57% had tertiary educational level. The income was around 1x (54.90%) to 2x (41.18%) minimum wage. Fifty-four percent showed normal weight, while 40% were overweight. The patients showed normal mean values of haematological parameters, and mean serum iron was 14.40 µM (SEM ± 0.83). The FFQ showed moderate correlation with the 3dFR (ρ = 0.5436, p = 0.0009), and the mean values of iron intake were 10.55(± 0.92) mg/day, recorded by FFQ, and 15.75(± 1.51) mg/day, recorded by 3dFR. The iron intake, recorded by FFQ, negatively correlated with serum iron (ρ = -0.3448, p = 0.0132), and did not have influence in the CD4+ T cell counts [e.B 0.99 (0.97-1.01, 95% confidence interval (CI), p = 0.2]. However, the iron intake showed a positive effect in HIV viral load [e.B 1.12 (1.02-1.25, 95%CI), p < 0.01]. MAIN CONCLUSIONS This study draws attention for the importance of iron intake nutritional counseling in people living with HIV. However, more studies are required to clarify the association between high iron intake and HIV infection and outcome.

Os enfermeiros na era da informação precisam desenvolver seus conhecimentos e habilidades para que se tornem competentes nessa área. O objetivo deste estudo foi verificar o conhecimento dos alunos matriculados no primeiro e no oitavo semestres do curso de graduação em enfermagem dos anos de 2008 e 2007, respectivamente, no que se refere à utilização de recursos da informática. Trata-se de uma pesquisa não experimental do tipo estudo survey descritivo exploratório usado para a coleta dos dados em um questionário baseado em um conjunto de competências em informática. Os resultados mostraram o baixo índice de conhecimentos em informática dos alunos que estão ingressando no curso de graduação. Contudo, na comparação dos aplicativos que os alunos têm maior dificuldade, entre os dois períodos avaliados, a maior porcentagem foi de alunos do oitavo semestre, demonstrando a necessidade da introdução do uso do computador na formação desses novos profissionais para sua posterior adaptação ao mercado de trabalho.

Nurses in the information age need to build their knowledge and abilities in order to be competent in this area. The objective of this study was to verify the knowledge of nursing freshmen (1st semester) and seniors (8th semester) registered in 2008 and 2007, respectively, regarding their ability to use informatics resources. This is a non-experimental, descriptive, exploratory survey. Data collection was performed using a questionnaire based on a set of competences in informatics. The results revealed a low rate of informatics knowledge among the freshmen. However, regarding the applications that students had the most difficulty to operate, between the two periods, seniors had the worst performance, which shows it is necessary to include computer classes in the preparation of these new professional, in order to prepare them for the work market.

Los enfermeros en la era informática necesitan construir conocimientos y habilidades con el objeto de ser competentes en dicha área. Este estudio objetivó verificar el conocimiento de alumnos matriculados en primer y octavo semestres del curso de graduación en enfermería de los años 2008 y 2007, respectivamente, en referencia a utilización de recursos informáticos. Investigación no experimental del tipo estudio survey, descriptivo, exploratorio, utilizándose para recolección de datos un cuestionario basado en un conjunto de competencias en informática. Los resultados demostraron el bajo índice de conocimientos informáticos de los alumnos que están ingresando al curso de graduación. Así y todo, en la comparación de las aplicaciones en que los alumnos tienen mayor dificultad, entre ambos períodos, el mayor porcentaje fue de alumnos de octavo semestre, demostrándose la necesidad de introducción de uso del computador en la formación de los nuevos profesionales para su posterior adaptación al mercado laboral.

Nurses in the information age need to build their knowledge and abilities in order to be competent in this area. The objective of this study was to verify the knowledge of nursing freshmen (1st semester) and seniors (8th semester) registered in 2008 and 2007, respectively, regarding their ability to use informatics resources. This is a non-experimental, descriptive, exploratory survey. Data collection was performed using a questionnaire based on a set of competences in informatics. The results revealed a low rate of informatics knowledge among the freshmen. However, regarding the applications that students had the most difficulty to operate, between the two periods, seniors had the worst performance, which shows it is necessary to include computer classes in the preparation of these new professional, in order to prepare them for the work market.

Los enfermeros en la era informática necesitan construir conocimientos y habilidades con el objeto de ser competentes en dicha área. Este estudio objetivó verificar el conocimiento de alumnos matriculados en primer y octavo semestres del curso de graduación en enfermería de los años 2008 y 2007, respectivamente, en referencia a utilización de recursos informáticos. Investigación no experimental del tipo estudio survey, descriptivo, exploratorio, utilizándose para recolección de datos un cuestionario basado en un conjunto de competencias en informática. Los resultados demostraron el bajo índice de conocimientos informáticos de los alumnos que están ingresando al curso de graduación. Así y todo, en la comparación de las aplicaciones en que los alumnos tienen mayor dificultad, entre ambos períodos, el mayor porcentaje fue de alumnos de octavo semestre, demostrándose la necesidad de introducción de uso del computador en la formación de los nuevos profesionales para su posterior adaptación al mercado laboral.

Os enfermeiros na era da informação precisam desenvolver seus conhecimentos e habilidades para que se tornem competentes nessa área. O objetivo deste estudo foi verificar o conhecimento dos alunos matriculados no primeiro e no oitavo semestres do curso de graduação em enfermagem dos anos de 2008 e 2007, respectivamente, no que se refere à utilização de recursos da informática. Trata-se de uma pesquisa não experimental do tipo estudo survey descritivo exploratório usado para a coleta dos dados em um questionário baseado em um conjunto de competências em informática. Os resultados mostraram o baixo índice de conhecimentos em informática dos alunos que estão ingressando no curso de graduação. Contudo, na comparação dos aplicativos que os alunos têm maior dificuldade, entre os dois períodos avaliados, a maior porcentagem foi de alunos do oitavo semestre, demonstrando a necessidade da introdução do uso do computador na formação desses novos profissionais para sua posterior adaptação ao mercado de trabalho.