Abstract Among the steps for the conversion of biomass into bioenergy, there is enzymatic hydrolysis. However, factors such as composition, formation of inhibitors, inhibition and enzymatic deactivation can affect the yield and productivity of this process. Lignocellulosic biomass is composed of cellulose, hemicellulose and lignin. However, lignin is organized in a complex and non-uniform way, promotes biomass recalcitrance, which repress the enzymatic attack on cellulose to be converted into glucose, and, consequently, the production of biofuel. Thus, a challenge in enzymatic hydrolysis is to model the reaction behavior. In this context, this study aims to evaluate the performance in enzymatic hydrolysis for the conversion of cellulose present in sugarcane bagasse into glucose. Therefore, modeling and optimization will be proposed to produce high glucose concentration rates. Therefore, a previously developed study will be used, in which the authors proposed a kinetic model for the hydrolysis step. However, as a differential to what has been proposed, the calculation will be carried out evaluating the evaporation, in order to maximize the response to the glucose concentration. Thus, considering evaporation and optimized kinetic parameters, it was possible to obtain high rates of glucose concentration at 204.23 $g.L^{-1