Cupuaçu (Theobroma grandiflorum Schum.) is an Amazonian fruit rich in antioxidants potential due to its phenolic compounds. Its seeds produce cupulate, a chocolate-like product, and the residue remains rich in biocompounds. This study aimed to optimize and compare microwave-assisted (MAE) and high pressure/temperature extraction (HPTE) of polyphenolic and flavonoid compounds from cupuaçu seed by-products. A Box-Behnken 33 factorial design assessed the influence of extraction time, solid/liquid ratio, and ethanol concentration. Responses included total polyphenol content, total flavonoid content, and anti-radical power via the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) method. In optimum conditions, MAE had the highest total polyphenol content and HPTE for flavonoids. Optimal conditions for both methods were 65% (m/v) ethanol concentration, 45 min extraction time, and 0.03 g mL−1 solid/liquid ratio. MAE and HPTE achieved greater recovery of (−)-epicatechin and (−)-epigallocatechin-3-gallate compared to percolation. Both techniques proved viable for recovering polyphenols from cupuaçu seed by-products, in less time and with less solvent. Thus, cupuaçu by-products can be a sustainable and functional food source, with MAE and HPTE providing high yields and quality extracts rich in polyphenols. Theobroma Schum. Schum cupulate chocolatelike chocolate like product biocompounds microwaveassisted microwave assisted (MAE pressuretemperature pressure temperature (HPTE byproducts. byproducts by products. products BoxBehnken Box Behnken 3 solidliquid solid liquid ratio concentration antiradical anti radical 2,2azinobis3ethylbenzothiazoline6sulphonic 22azinobis3ethylbenzothiazoline6sulphonic azinobisethylbenzothiazolinesulphonic 2,2 azino bis ethylbenzothiazoline 6 sulphonic 2 acid method flavonoids 65 m/v mv m v (m/v 4 003 0 03 0.0 mL1 mL 1 mL− −epicatechin epicatechin − −epigallocatechin3gallate epigallocatechin3gallate epigallocatechingallate epigallocatechin gallate percolation byproducts, products, solvent Thus source 2azinobis3ethylbenzothiazoline6sulphonic azinobis 22 2, 00 0.

Iron is an important ion that regulates many metabolic functions in the human body, and its deficiency or overload can cause serious health problems. In view of this, the development of tools that can quantify iron in aqueous media is quite valuable. A new material based on a metal-organic framework (MOF) and a calixarene derivative (CALIX), here named CALIX@UiO-66-NH2, was successfully prepared and used as a luminescent sensor for iron ions in a water matrix containing various metal ions. The post-synthetic modification was demonstrated to be efficient on the MOF (UiO-66-NH2) modification, leading to a hybrid platform. The effect of the combination of both components (MOF and CALIX) led to a platform more sensitive to iron detection than the independent compounds. The CALIX@UiO-66-NH2 sensor shows a linear response toward Fe3+ ions with satisfactory linearity (R2 = 0.994) within the concentration range of 6.74 × 10−6−1.72 × 10−5 mol L−1. The limit of detection was determined to be as low as 1.6 mg L−1. The data demonstrate that CALIX@UiO-66-NH2 is an excellent probe for detecting Fe3+, as confirmed by almost complete fluorescence suppression. body problems this valuable metalorganic organic CALIX, CALIX , (CALIX) CALIX@UiO66NH2, CALIXUiO66NH2 CALIXUiONH CALIX@UiO 66 NH2, UiO NH2 NH postsynthetic post synthetic UiO66NH2 UiONH (UiO-66-NH2 compounds CALIX@UiO66NH2 CALIX@UiO-66-NH Fe3 Fe R2 R (R 0.994 0994 0 994 674 6 74 6.7 106172 10 1 72 10−6−1.7 105 5 10− L1 L L−1 16 1. suppression (CALIX CALIXUiO CALIXUiO66NH UiO66NH (UiO-66-NH CALIX@UiO66NH 0.99 099 99 67 7 6. 10617 10−6−1. L− 0.9 09 9 1061 10−6−1 0. 106 10−6− 10−6

In the present work, three series of cinnamic (1), p-coumaric (2) and ferulic (3) esters containing different side-chains such as ethyl (1a-3a), n-propyl (1b-3b), n-butyl (1c-3c), n-pentyl (1d-3d), n-hexyl (1e-3e), and n-heptyl (1f-3f) were prepared, tested for activity against trypomastigote forms of the parasite Trypanosoma cruzi and toxicity against NCTC cells. Obtained results indicated that the presence of p-coumaric or ferulic moieties associated with C4-C7 linear side-chains play an important role in the bioactivity against T. cruzi since compounds 2c-2f and 3d-3f were found to be the most active derivatives with a half maximal effective concentration (EC50) value ranging from 12.8 to 1.7 μM, superior to that determined for the positive control benznidazole (EC50 = 16.4 μM). Additionally, machine learning and multivariate statistical analyses identified molecular features correlated with biological activity, emphasizing the importance of side-chain length and lipophilicity, highlighting the significance of the molecular structure of phenylpropanoid derivatives in the activity against T. cruzi. work 1, 1 , (1) pcoumaric p coumaric 2 (2 3 (3 sidechains side chains 1a3a, 1a3a aa 1a 3a (1a-3a) npropyl n propyl 1b3b, 1b3b bb 1b 3b b (1b-3b) nbutyl butyl 1c3c, 1c3c cc 1c 3c c (1c-3c) npentyl pentyl 1d3d, 1d3d dd 1d 3d d (1d-3d) nhexyl hexyl 1e3e, 1e3e ee 1e 3e e (1e-3e) nheptyl heptyl 1f3f ff 1f 3f f (1f-3f prepared cells C4C7 CC C4 C7 C C4-C T 2c2f cf 2c 2f 3d3f df EC50 EC 128 12 8 12. 17 7 1. μM (EC5 164 16 4 16. μM. . μM) Additionally sidechain chain lipophilicity (1 ( (1a-3a (1b-3b (1c-3c (1d-3d (1e-3e C4C EC5 (EC

This study details the preparation, antileishmanial assay, and in silico analysis of twenty 2-hydroxy-3-(1,2,3-triazolyl)propyl vanillin derivatives. These compounds were synthesized in three steps and evaluated against Leishmania infantum, Leishmania amazonensis, and Leishmania braziliensis promastigotes. Compounds 3s and 3t were the most effective, showing good activity against all Leishmania species tested. Molecular docking indicated that all compounds bind favorably to the sterol 14α-demethylase (CYP51) enzyme from L. infantum. ADMET (absorption, distribution, metabolism, excretion and toxicity) analysis indicated good oral bioavailability, non blood-brain barrier penetration, and high gastrointestinal absorption. Posaconazole and compounds 3e, 3s, and 3t remained stable in the CYP51 binding region during 100 ns molecular dynamics (MD) simulations. Root mean square deviation (RMSD) and root mean square fluctuations (RMSF) analyses from the MD trajectory revealed significant conformational fluctuations of the CYP51 N-terminal, suggesting occasional expulsion of 3e, potentially explaining its higher IC50 (half-maximal inhibitory concentration) values. Pairwise decomposition analyses from molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) calculations highlighted the importance of hydrophobic residues in interacting with the synthesized derivatives. preparation assay 2hydroxy31,2,3triazolylpropyl 2hydroxy3123triazolylpropyl hydroxytriazolylpropyl 2 hydroxy 3 1,2,3 triazolyl propyl 1 derivatives infantum amazonensis promastigotes s t effective tested 14αdemethylase αdemethylase 14α demethylase α CYP (CYP51 L absorption, absorption (absorption distribution metabolism toxicity bioavailability bloodbrain blood brain penetration 3e e CYP5 10 (MD simulations RMSD (RMSD RMSF (RMSF Nterminal, Nterminal N terminal, terminal N-terminal IC IC5 halfmaximal half maximal concentration values PoissonBoltzmann Poisson Boltzmann MM/PBSA MMPBSA MM PBSA (MM/PBSA 2hydroxy31 3triazolylpropyl triazolylpropyl 123 1,2, (CYP5 2hydroxy3 12 1,2 (CYP 2hydroxy 1,

Bredemeyera Willd. is a genus of the Polygalaceae family, occurring in Brazil. This research aims to evaluate the phytochemical and antifungal potential, in vitro and in silico, of Bredemeyera brevifolia extracts. The identification of fatty acids by gas chromatography-mass spectrometry and their quantification by gas chromatography-flame ionization detector showed that palmitic (36.55 mg g−1), margaric (3.66 mg g−1), linolelaidic (108.82 mg g−1), oleic (7.86 mg g−1), stearic (41.11 mg g−1), dihomo-γ-linolenic (85.48 mg g−1), and arachidic (209.00 mg g−1) acids are present in the hexane extract. From the chloroform extract, 1-hydroxy-3,7-dimethoxyxanthone was isolated and identified. The minimum inhibitory concentration assay with methanolic extract demonstrated antifungal activity against species of the genus Candida, Sporothrix, and Trichophyton for up to 96 h, with values ranging from 64-1024 μg mL−1. The in silico study with the chemical compounds of this species showed fungal inhibition potential of sterol-14-α-demethylase, highlighting 1-hydroxy-3,7-dimethoxyxanthone, which presented an affinity energy of -8.8 kcal mol−1, greater than that observed for fluconazole (−8.3 kcal mol−1). The fatty acids interacted with the active site of the enzyme but presented energies ≥ −7.4 kcal mol−1. Compounds isolated from this species have pharmacokinetic potential for the development of pharmaceutical formulations. Willd family Brazil extracts chromatographymass chromatography mass chromatographyflame flame 36.55 3655 36 55 (36.5 g−1, g1 g g−1 , 1 3.66 366 3 66 (3.6 108.82 10882 108 82 (108.8 7.86 786 7 86 (7.8 41.11 4111 41 11 (41.1 dihomoγlinolenic dihomo γ linolenic 85.48 8548 85 48 (85.4 209.00 20900 209 00 (209.0 1hydroxy3,7dimethoxyxanthone 1hydroxy37dimethoxyxanthone hydroxydimethoxyxanthone hydroxy 3,7 dimethoxyxanthone identified Candida Sporothrix 9 h 641024 64 1024 64-102 mL1 mL mL−1 sterol14αdemethylase, sterol14αdemethylase sterolαdemethylase sterol 14 α demethylase, demethylase sterol-14-α-demethylase 1hydroxy3,7dimethoxyxanthone, dimethoxyxanthone, 8.8 88 8 -8. mol1 mol mol−1 −8.3 83 (−8. . mol−1) 74 4 −7. formulations 36.5 365 5 (36. g− 3.6 6 (3. 108.8 1088 10 (108. 7.8 78 (7. 41.1 411 (41. 85.4 854 (85. 209.0 2090 20 0 (209. 1hydroxy3 7dimethoxyxanthone 37 3, 64102 102 64-10 mL− αdemethylase 8. -8 mol− −8. (−8 −7 36. (36 3. (3 108. (108 7. (7 41. (41 85. (85 209. 2 (209 1hydroxy 6410 64-1 - −8 (− − ( (10 (4 (8 (20 641 64- (1 (2

ABSTRACT Introduction: Although nerves and vessels of the penis play important role in erection, there are few studies on their development in human fetus. Therefore, the objective of the present study is to analyze, quantitatively, in the corpora cavernosa and corpus spongiosum, the development of the nerves and vessels in the fetal penis at different gestational ages. Material and Methods: Fifty-six fresh, macroscopically normal human fetuses aged from 13 to 36 weeks post-conception (WPC) were used. Gestational age was determined by the foot length criterion. Penises were immediately fixed in 10% formalin, and routinely processed for paraffin embedding, after which tissue sections from the mid-shaft were obtained. We used immunohistochemical staining to analyze the nerves and vessels in the corpus cavernous and in the corpus spongiosum. These elements were identified and quantified as percentage by using the Image-J software. Results: The quantitative analysis showed that the percentage of nerves varied from 3.03% to 20.35% in the corpora cavernosa and from 1.89% to 23.88% in the corpus spongiosum. The linear regression analysis indicated that nerves growth (incidence) in the corpora cavernosa and corpus spongiosum correlated significantly and positively with fetal age (r2=0.9421, p<0.0001) and (r2=0.9312, p<0.0001), respectively, during the whole fetal period studied. Also, the quantitative analysis showed that the percentage of vessels varies from 2.96% to 12.86% in the corpora cavernosa and from 3.62% to 14.85% in the corpus spongiosum. The linear regression analysis indicated that vessels growth (appearance) in the corpora cavernosa and corpus spongiosum correlated significantly and positively with fetal age (r2=0.8722, p<0.0001) and (r2=0.8218, p<0.0001), respectively, during the whole fetal period studied. In addition, the linear regression analysis demonstrated a more intense growth rate of nerves in the corpus spongiosum during the 2nd trimester of gestation, when compared with nerves in the corpora cavernosa. In addition, the linear regression analysis demonstrated a more intense growth rate of vessels in the corpus spongiosum when compared with the corpora cavernosa, during the whole fetal period studied. Conclusions: In the fetal period, the human penis undergoes major developmental changes, notably in the content and distribution of nerves and vessels. We found strong correlation between nerves and vessels growth (amount) with fetal age, both in the corpora cavernosa and corpus spongiosum. There is significant greater proportional number of nerves than vessels during the whole fetal period studied. Also, nerves and vessels grow in a more intense rate than that of the corpora cavernosa and corpus spongiosum areas. Introduction erection fetus Therefore quantitatively ages Methods Fiftysix Fifty six fresh 1 3 postconception post conception WPC (WPC criterion 10 formalin embedding midshaft mid shaft obtained ImageJ Image J software Results 303 03 3.03 2035 20 35 20.35 189 89 1.89 2388 23 88 23.88 incidence (incidence r2=0.9421, r209421 r r2 0 9421 (r2=0.9421 p<0.0001 p00001 p 0001 r2=0.9312, r209312 9312 (r2=0.9312 p<0.0001, , respectively studied Also 296 2 96 2.96 1286 12 86 12.86 362 62 3.62 1485 14 85 14.85 appearance (appearance r2=0.8722, r208722 8722 (r2=0.8722 r2=0.8218, r208218 8218 (r2=0.8218 addition nd gestation Conclusions changes amount (amount areas 30 3.0 203 20.3 18 8 1.8 238 23.8 r2=0.9421 r20942 942 (r2=0.942 p<0.000 p0000 000 r2=0.9312 r20931 931 (r2=0.931 29 9 2.9 128 12.8 6 3.6 148 14.8 r2=0.8722 r20872 872 (r2=0.872 r2=0.8218 r20821 821 (r2=0.821 3. 20. 1. 23. r2=0.942 r2094 94 (r2=0.94 p<0.00 p000 00 r2=0.931 r2093 93 (r2=0.93 2. 12. 14. r2=0.872 r2087 87 (r2=0.87 r2=0.821 r2082 82 (r2=0.82 r2=0.94 r209 (r2=0.9 p<0.0 p00 r2=0.93 r2=0.87 r208 (r2=0.8 r2=0.82 r2=0.9 r20 (r2=0. p<0. p0 r2=0.8 r2=0. (r2=0 p<0 r2=0 (r2= p< r2= (r2 (r

RESUMO Combinar refrigeração com embalagem é uma técnica comumente utilizada para frutas destinadas à exportação. Assim, o objetivo deste estudo foi adequar o manejo pós-colheita de cultivares de batata-doce biofortificadas, utilizando embalagem e refrigeração, com foco em atingir mercados de exportação distantes e mantendo a qualidade comercial. As batatas-doces das cultivares BRS-Amélia e Beauregard foram selecionadas após a colheita, lavadas e embaladas. O delineamento experimental utilizado foi inteiramente casualizado, em arranjo fatorial 4 × 7 composto por 4 tratamentos (cultivar BRS-Amélia com e sem embalagem; e cultivar Beauregard com e sem embalagem) e 7 avaliações nas condições de armazenamento: aos 0, 7, 14 (sob refrigeração a aproximadamente 8 °C), 17, 20, 23 e 26 dias (sob condições de temperatura ambiente a aproximadamente 25 °C). Cada unidade experimental continha 500 g de tubérculos. Os resultados mostraram que o armazenamento refrigerado aliado à embalagem prolongou a vida útil da batata-doce avaliada: 17 dias para BRS-Amélia e 26 dias para a cultivar Beauregard. A cultivar BRS-Amélia manteve maior firmeza, solúveis sólidos e teor de carboidratos, croma e atividade antioxidante (método FRAP) e teor de fenólicos totais, tanto nos tecidos crus quanto cozidos. A cultivar Beauregard apresentou menor desidratação e maior estabilidade nos teores de vitamina C, fenólicos totais, carotenóides totais, carboidratos solúveis e amido em batatas cruas e cozidas. Portanto, a combinação de embalagem e refrigeração preservou a qualidade comercial da batata-doce biofortificada para os mercados de exportação. Assim póscolheita pós colheita batatadoce batata doce biofortificadas batatasdoces doces BRSAmélia BRS Amélia embaladas casualizado 0 1 sob °C, C °C , °C) 20 2 °C. . 50 tubérculos avaliada firmeza método FRAP totais cozidos cozidas Portanto 5

ABSTRACT Combining refrigeration with packaging is a commonly used technique for fruits intended for export. Thus, the objective of this study was to adapt the post-harvest management of biofortified sweet potato cultivars, using packaging and refrigeration, focused on reaching distant export markets while maintaining the commercial quality. Sweet potatoes of the cultivars BRS-Amélia and Beauregard were selected after harvesting, washed, and packaged. The experimental design used was completely randomized, in a 4 × 7 factorial arrangement consisted of 4 treatments (cultivar BRS-Amélia with and without packaging; and cultivar Beauregard with and without packaging) and 7 evaluations under storage conditions: at 0, 7, 14 (under refrigeration at approximately 8 °C), 17, 20, 23, and 26 days (under room temperature conditions at approximately 25 °C). Each experimental unit contained 500 g of tubbers. The results showed that the refrigerated storage combined with packaging extended the shelf life of the evaluated sweet potatoes: 17 days for BRS-Amélia and 26 days for the cultivar Beauregard. The BRS-Amélia cultivar maintained higher firmness, soluble solids and carbohydrate contents, chroma, and antioxidant activity (FRAP method), and total phenolic contents, in both raw and cooked tissues. The cultivar Beauregard presented less dehydration and greater stability in vitamin C, total phenolic, total carotenoid, soluble carbohydrate, and starch contents in raw and cooked potatoes. Therefore, the combination of packaging and refrigeration preserved the commercial quality of biofortified sweet potatoes for export markets. Thus postharvest post harvest BRSAmélia BRS Amélia harvesting washed packaged randomized 0 1 °C, C °C , °C) 20 23 2 °C. . 50 tubbers firmness chroma FRAP method, method method) tissues carotenoid Therefore 5

RESUMO O manejo dos níveis de condutividade elétrica é um dos fundamentos necessários para se obter maior produção e melhor qualidade de hortaliças. O objetivo deste trabalho foi avaliar o efeito da condutividade elétrica da solução nutritiva na cultura da couve-rábano cultivada em sistema semi-hidropônico. O delineamento experimental foi em blocos casualizados com cinco tratamentos associados à condutividade elétrica da solução nutritiva (1,31; 1,71; 2,37; 2,98 e 3,75 dS m-1) e quatro repetições. As plantas foram colhidas 78 dias após o transplante e avaliadas quanto ao crescimento, produção e qualidade pós-colheita. Solução nutritiva com condutividade elétrica (CE) de 1,31 dS m-1 proporciona maior produção de couve-rábano roxa. O aumento da CE reduziu linearmente a maioria das variáveis analisadas (área foliar, massa fresca de folhas, bulbos e parte aérea, massa seca de folhas e totais, volume e firmeza de bulbos), com perdas mais expressivas para as variáveis massa fresca de bulbos (50,54%) e volume de bulbos (57,37%). A utilização de solução nutritiva com CE entre 2,3 e 2,9 dS m-1 aumentou o teor de vitamina C e a acidez titulável. hortaliças couverábano couve rábano semihidropônico. semihidropônico semi hidropônico. hidropônico semi-hidropônico 131 1 31 (1,31 1,71 171 71 2,37 237 2 37 298 98 375 3 75 3,7 m1 m repetições 7 crescimento póscolheita. póscolheita pós colheita. colheita pós-colheita (CE 1,3 m- roxa área foliar aérea totais bulbos, , bulbos) 50,54% 5054 50 54 (50,54% 57,37%. 5737 57,37% . 57 (57,37%) 23 2, 29 9 titulável 13 (1,3 1,7 17 3, 1, 50,54 505 5 (50,54 573 57,37 (57,37% (1, 50,5 (50,5 57,3 (57,37 (1 50, (50, 57, (57,3 ( (50 (57, (5 (57

ABSTRACT Managing electrical conductivity levels is one of the fundamentals required to obtain greater production and better quality of vegetables. The aim of this study was to evaluate the effect of electrical conductivity of the nutrient solution on the kohlrabi crop cultivated in semi-hydroponic system. A randomized block design was used with five treatments associated with the electrical conductivity of the nutrient solution (1.31, 1.71, 2.37, 2.98, and 3.75 dS m-1) and four replications. The plants were harvested 78 days after transplanting and evaluated for growth, production, and postharvest quality. Nutrient solution with electrical conductivity (EC) of 1.31 dS m-1 promotes greater production of purple kohlrabi. The increase in EC reduced linearly most of the analyzed variables (leaf area, fresh mass of leaves, bulb and shoot, dry mass of leaves and total, bulb volume and firmness), with more significant losses for the bulb fresh mass (50.54%) and bulb volume (57.37%) variables. The use of nutrient solution with EC between 2.3 and 2.9 dS m-1 increased the vitamin C content and the titratable acidity. vegetables semihydroponic semi hydroponic system 1.31, 131 1 31 (1.31 171 71 1.71 237 2 37 2.37 298 98 2.98 375 3 75 3.7 m1 m replications 7 growth (EC 1.3 m- leaf area shoot total firmness, firmness , firmness) 50.54% 5054 50 54 (50.54% 57.37% 5737 57 (57.37% 23 2. 29 9 acidity 13 (1.3 17 1.7 3. 1. 50.54 505 5 (50.54 57.37 573 (57.37 (1. 50.5 (50.5 57.3 (57.3 (1 50. (50. 57. (57. ( (50 (57 (5

Resumo Horismenus camobiensis sp. nov. (Hymenoptera: Eulophidae) é descrita com base em dados morfológicos, moleculares e ecológicos; esta nova espécie Chalcididae atua como hiperparasitoide de Opsiphanis invirae (Hübner, 1818) (Lepidoptera: Nymphalidae) em pupas de seu parasitoide Cotesia invirae Salgado-Neto and Whitfield, 2019 (Hymenoptera: Braconidae). Caracteres diagnósticos morfológicos e moleculares e ilustrações de H. camobiensis são fornecidos.

Abstract Horismenus camobiensis sp. nov. (Hymenoptera: Eulophidae), is described based on morphological, molecular and ecological data; this new species of chalcid wasp acts as hyperparasitoid of Opsiphanis invirae (Hübner, 1818) (Lepidoptera: Nymphalidae) in its parasitoid Cotesia invirae Salgado-Neto and Whitfield, 2019 (Hymenoptera: Braconidae). Diagnoses with morphological and molecular characters and illustrations are provided.

Resumo As comunidades quilombolas, estão presentes em diversos estados brasileiros, vivendo em condições de saúde mais precárias. Isto ocorre por conta do isolamento geográfico, das limitações de acesso e da falta de qualidade no serviço quando este é prestado. Nesse sentido, o objetivo do estudo foi analisar a qualidade de vida de mulheres de uma comunidade quilombola do nordeste brasileiro. Estudo observacional, transversal e descritivo. Foram entrevistadas 160 mulheres adultas, através de um formulário para a coleta de perfil e do questionário de Qualidade de Vida WHOQOL – bref. Foi observado que as mulheres tinham em média 40,7 anos (±17,25), casadas, autodeclaradas negras, com fundamental incompleto, do lar, sem renda, com moradia de alvenaria, própria, com até 6 cômodos, abastecidas por caixa de água comunitária, tratada. A Qualidade de Vida, apresentou escores medianos nos domínios: físico (3,18), psicológico (3,4), relações sociais (3,45) e meio ambiente (2,59). Com a realização desta pesquisa foi possível caracterizar a comunidade quilombola de Santa Luzia do Norte-AL quanto as dificuldades de acesso a saúde e geração de renda, fatos que repercutem na sua condição de saúde. Os problemas descritos neste estudo podem contribuir para que ações de saúde sejam planejadas e efetivadas com o intuito de melhorar as condições socioeconômicas e de saúde nessa comunidade, considerando-se o contexto social, político e ambiental, valorizando seus saberes e práticas tradicionais.

Abstract Quilombola communities are present in many Brazilian states living in precarious health conditions. This is due to geographic isolation, limitations to the access of the area in which they live in, and the lack of quality in the service when it is needed to be provided. Therefore, the aim of this study was to analyze the quality of life of women from a quilombola community in northeastern Brazil. It is an observational, cross-sectional and descriptive study. 160 adult women were first interviewed through a form to collect a profile and then it was applied the WHOQOL Quality of Life questionnaire – bref. It was observed that the women were on average 40.7 years old (±17.25), married, self-declared black, who did not finish elementary school, housewife, had no income, with their own masonry house, with up to 6 rooms, supplied by a box of community treated water. Quality of Life had median scores in the domains: physical (3.18), psychological (3.4), social relationships (3.45) and environment (2.59). With this research, it was possible to characterize the quilombola community of Santa Luzia do Norte-AL regarding the difficulties of access to health and income generation, issues that affect their health condition. The problems described in this study can contribute to health actions being planned and carried out in order to improve socioeconomic and health conditions in this community, considering the social, political and environmental context, valuing their traditional knowledge and practices.

Resumo A melhoria da germinação de plantas é fundamental para garantia de mudas de melhor qualidade. Assim, objetivou-se avaliar se o condicionamento de sementes com qualidade de luz (light quality - LIQ) e extrato aquoso de tubérculos de Cyperus rotundus (AEC) modula a germinação e o crescimento inicial de plântulas de Moringa oleifera. Utilizou-se delineamento inteiramente casualizado, em esquema fatorial 4x4, sendo quatro condições de LIQ (luz branca, azul, vermelha e vermelho distante) e quatro concentrações de AEC (0, 25, 50 e 100%). O condicionamento de sementes com luz vermelha reduziu o tempo médio de emergência, enquanto que as luzes azul, vermelha e vermelho extremo associadas a 50% de extrato aquoso de C. rotundus aumentaram o comprimento inicial da parte aérea e o acúmulo de pigmentos fotossintéticos. Condicionamento de sementes com luz azul induziu a formação de plântulas com menor comprimento final da parte aérea, no entanto, a aplicação de 100% de extrato aquoso de C. rotundus reverteu o menor crescimento. A luz branca associada às concentrações de 50 e 100% de fitormônios promoveu maior taxa de crescimento relativo da parte aérea de plântulas. Nossa pesquisa mostra que o condicionamento de sementes com radiação espectral de luz e extrato aquoso de tubérculos de Cyperus rotundus modula a germinação e o crescimento inicial de plântulas de Moringa oleifera. Mais trabalhos precisam ser feitos para determinar os compostos do AEC responsáveis que que atuam como fitormônios e são responsáveis pelo crescimento inicial.

Abstract Improving plant germination is essential to guarantee better quality seedlings. Thus, this research aimed to evaluate whether the seed priming with light quality (LIQ) and the aqueous extract of Cyperus rotundus (AEC) tuber could modulate the germination and initial growth of Moringa oleifera L. seedlings. The experimental design was a completely randomized in the 4x4 factorial scheme, composed of four LIQ conditions (white, blue, red, and distant red light) and four AEC concentrations (0, 25, 50 and 100%). Seed priming with red light reduced the average emergence time, while blue, red, and extreme red lights associated with 50% of aqueous extract of C. rotundus increased shoot initial length and photosynthetic pigment accumulation. Seed priming with blue light resulted in seedlings with a shorter final shoot length. However, application of 100% of aqueous extract of C. rotundus reversed this. The white light in combination with concentrations of 50 and 100% of AEC promoted a higher relative shoot growth rate of seedlings. The research revealed that seed priming with light quality and aqueous extracts of C. rotundus tubers modulates the germination and initial growth of M. oleifera seedlings. More work needs to be done to determine the responsible compounds in AEC that is responsible for priming growth as phytohormones.

Resumo O conteúdo de dopamina nas estruturas cerebrais tem sido relacionado à excitabilidade neuronal e várias abordagens têm sido utilizadas para estudar este fenômeno durante o período de vulnerabilidade às crises. No presente trabalho, descrevemos os efeitos da depleção de dopamina após a administração de 6-hidroxidopamina (6-OHDA) na região pars compacta da substância negra de ratos submetidos ao modelo de epilepsia com pilocarpina. A susceptibilidade ao estado de mal epiléptico induzido pela pilocarpina, bem como a frequência de crises espontâneas e recorrentes durante o período crônico do modelo foi determinada. Sendo o hipocampo uma das principais estruturas afetadas no desenvolvimento desse modelo experimental de epilepsia, os níveis de dopamina nessa região foram determinados após a administração da droga. No primeiro experimento, 62% (15/24) dos ratos pré-tratados com 6-OHDA e 45% (11/24) daqueles que receberam ácido ascórbico como solução controle evoluíram para crises límbicas motoras e para o estado de mal epiléptico, após a administração de pilocarpina. A gravidade das crises durante o período agudo do modelo foi significativamente maior nos ratos epilépticos experimentais (56,52%) do que nos ratos controle (4,16%). No segundo experimento, não houve diferença significante entre os grupos quanto à frequência de crises na fase crônica do modelo. Nossos dados mostraram que a dopamina pode desempenhar um papel importante na gravidade das crises na fase aguda da pilo, o que parece ser exercido por sua ação inibitória da dopamina sobre a expressão motora das crises.

Abstract The dopamine content in cerebral structures has been related to neuronal excitability and several approaches have been used to study this phenomenon during seizure vulnerability period. In the present work, we describe the effects of dopamine depletion after the administration of 6-hidroxidopamine (6-OHDA) into the substantia nigra pars compacta of male rats submitted to the pilocarpine model of epilepsy. Susceptibility to pilocarpine-induced status epilepticus (SE), as well as spontaneous and recurrent seizures (SRSs) frequency during the chronic period of the model were determined. Since the hippocampus is one of main structures in the development of this experimental model of epilepsy, the dopamine levels in this region were also determined after drug administration. In the first experiment, 62% (15/24) of 6-OHDA pre-treated rats and 45% (11/24) of those receiving ascorbic acid as control solution progressed to motor limbic seizures evolving to SE, after the administration of pilocarpine. Severeness of seizures during the model´s the acute period, was significantly higher in epileptic experimental rats (56.52%), than in controls (4.16%). In the second experiment, the frequency of seizures in the model’s chronic phase did not significantly change between groups. Our data show that dopamine may play an important role on seizure severity in the pilo’s model acute period, which seems to be due to dopamine inhibitory action on motor expression of seizure.

Resumo A leishmaniose visceral é uma doença tropical negligenciada. Foram avaliadas a distribuição espacial dos casos de leishmaniose visceral no estado de Alagoas. Todos os casos de LV, registrados pela secretaria de saúde, foram analisados e georreferenciados. Entre 2008 e 2017, 97,1% dos municípios apresentaram classificação esporádica de transmissão. Com a evolução temporal, a incidência de casos de leishmaniose visceral se concentrou na maioria dos municípios da microrregião de Santana do Ipanema-AL. A análise espaço-tempo, se considerada, pode promover o aprimoramento das ações de vigilância e controle da leishmaniose visceral.

Abstract Visceral leishmaniosis is a neglected tropical disease. We evaluated the spatial distribution of cases of visceral leishmaniosis in the state of Alagoas, Brazil. All cases of VL, registered by the health department, were analyzed and georeferenced. Results: Between 2008 and 2017, 97.1% of the municipalities presented sporadic classification of transmission. With temporal evolution, the incidence of cases of visceral leishmaniosis was concentrated in most municipalities in the microregion of Santana do Ipanema-AL. Space-time analysis, if considered, may promote the improvement of surveillance and control actions of visceral leishmaniosis.