ABSTRACT Purpose: This study aimed to evaluate the perception and degree of satisfaction of blind individuals regarding an electronic cane prototype with a wearable haptic interface. Methods: Two scenarios with different obstacles were created to conduct tests with the canes (the user’s cane and the prototype one). The perception and satisfaction of participants regarding the electronic cane were assessed using a questionnaire, the number of collisions during the tests, and the time each individual took to complete the course in each scenario. Results: Ten blind individuals who used the white cane participated in this study. Eight were males, and two were females. Their age ranged from 23 to 43 (average 32.3 ± 7.13 years and median 32 years). There was a tendency for fewer collisions with ground obstacles when the electronic cane was used than when the white cane was used. However, there was no statistically significant difference between the number of collisions and the course completion time in each scenario with either canes tested. Conclusion: Overall, the perception and satisfaction of individuals regarding the prototype used were positive. Purpose interface Methods users user s one. one . one) questionnaire Results males females 2 4 average 323 3 32. 713 7 13 7.1 years. years) However tested Conclusion Overall positive 71 1 7.

Worldwide, millions of people suffer from neurological and psychiatric conditions, such as schizophrenia, depression, and neurodegenerative diseases. These conditions are often linked to hyperactivity of monoamine oxidase B (MAO-B) in the brain, and inhibiting this enzyme can reduce oxidative stress, stabilize neurotransmitter levels, and alleviate symptoms. In this context, a pharmacophoric model was created using PharmaGist with 31 hits extracted from BindingDB to enable a pharmacophoric-based search of potential MAO-B inhibitors in ZincPharmer’s chemical space, followed by the building of 10 conformers for each molecule in the software OMEGA, screening for structural and electrostatic similarity to safinamide using ROCS (Rapid overlay of chemical structures) and EON (Electrostatic similarity for lead-hopping), respectively. In addition, the molecular docking with Gold using the score function was performed to rank the ligands with the highest probabilities of affinity to MAO-B and then discriminate those with suitable physical chemical, pharmacokinetic, and toxicological features using the tools QikProp, SwissADME, and DEREK. Four potential ligands with selective MAO-B inhibitor properties were identified, which show promise as future therapeutic agents for MAO-B-related disorders. Worldwide schizophrenia depression diseases MAOB MAO (MAO-B brain stress levels symptoms context 3 pharmacophoricbased based ZincPharmers ZincPharmer s space 1 OMEGA Rapid structures Electrostatic leadhopping, leadhopping lead hopping , lead-hopping) respectively addition pharmacokinetic QikProp SwissADME DEREK identified MAOBrelated related disorders lead-hopping

ABSTRACT: The main transmission route of bovine coronavirus (BCoV) is direct contact with secretions and feces, and potentially also via contaminated surfaces. Following PRISMA guidelines, a systematic review was conducted to evaluate the presence of BCoV on environmental surfaces. A literature search, conducted between March and April 2023, utilized databases including Web of Science, PubMed, Scopus, Science Direct, and Springer. The review adhered to the PEO structure: Population - environmental surfaces; Exposure - contact with BCoV; Outcome - identification of presence or infectivity. Primary data were recorded using an extraction form organizing methods for detecting BCoV presence, assessing BCoV infectivity, types of surfaces evaluated, and study type. Search terms “Bovine coronavirus” and “BCoV” yielded 2703 articles. After removing 964 duplicates and excluding 1546 articles not mentioning BCoV in titles, 193 studies underwent abstract reading. Following exclusion criteria not addressing BCoV presence in the environment, three articles were selected for comprehensive review. These articles identified BCoV presence on various types of surfaces, with detection possible up to 81 hours after contamination, depending on surface type. Despite limited studies on BCoV presence on surfaces, findings suggested potential transmission via contaminated surfaces due to the virus’s ability to remain infectious for up to 24 hours on fomites. This review underscores the need for further research on BCoV persistence in farm environments, an area currently lacking focused studies. ABSTRACT (BCoV feces guidelines search 2023 PubMed Scopus Direct Springer structure infectivity evaluated type Bovine “BCoV 270 96 154 titles 19 reading environment 8 contamination viruss virus s 2 fomites environments 202 27 9 15 1 20

RESUMO: A principal via de transmissão do coronavírus bovino (BCoV) é o contato direto com secreções e fezes, podendo ocorrer também por meio de superfícies contaminadas. Uma revisão sistemática, seguindo as recomendações PRISMA, foi realizada para avaliar a presença de BCoV em superfícies ambientais. A busca na literatura foi realizada em março e abril de 2023 e as bases de dados utilizadas foram Web of Science, PubMed, Scopus, Science Direct e Springer. A revisão sistemática seguiu a estrutura PEO: População - superfícies ambientais; Exposição - contato com BCoV; Resultado - identificação da presença ou infectividade de BCoV. As informações primárias foram registradas por meio de um formulário de extração, organizando métodos para detectar a presença de BCoV, avaliar a infectividade de BCoV, tipos de superfícies avaliadas e tipo de estudo. Os termos de pesquisa “coronavírus bovino” e “BCoV” resultaram em 2703 artigos. Após remover 964 duplicatas e excluir 1546 artigos que não mencionavam o BCoV nos títulos, 193 estudos foram submetidos à leitura de resumos. Seguindo os critérios de exclusão, que não abordavam a presença do BCoV no ambiente, três artigos foram selecionados para revisão abrangente. Nestes artigos foi identificada a presença de BCoV em vários tipos de superfícies, sendo possível a detecção até 81 horas após a contaminação, dependendo do tipo de superfície. Apesar do número limitado de estudos que investigam a presença de BCoV em superfícies, os resultados sugerem o potencial de transmissão de BCoV por meio de superfícies contaminadas, devido à capacidade do vírus permanecer infeccioso por até 24 horas em fômites. Esta revisão destaca a necessidade de mais pesquisas sobre a persistência de BCoV em locais de criação de bovinos, uma área atualmente carente de estudos específicos. RESUMO (BCoV fezes contaminadas PRISMA ambientais 202 PubMed Scopus Springer PEO extração estudo “BCoV 270 96 154 títulos 19 resumos exclusão ambiente abrangente 8 contaminação superfície 2 fômites bovinos específicos 20 27 9 15 1

ABSTRACT Objective: To evaluate the factors associated with the fear of dental students infecting their relatives with COVID-19 as a result of clinical practice. Material and Methods: Online questionnaires were sent by e-mail and social media to dental students from a Brazilian School of Dentistry. The outcome was "fear of infecting the family." The independent variables were sociodemographic data, personal protective equipment use, adoption of infection control measures, and knowledge and feelings about the pandemic and the future of Dentistry. Data analysis used Pearson's Chi-square and Fisher's exact tests (p≤0.05). Results: With 65 participants, the prevalence of fear was 61.5%. Measures to prevent COVID-19, such as frequent hand cleansing (p=0.028) and avoiding the use of aerosol-generating instruments (p=0.027), not having or not knowing if they have been infected by the disease (p=0.038), and feelings of more significant anxiety during the pandemic period (p=0.047), and that Dentistry will undergo major post-pandemic changes (p=0.020) were associated with fear. Conclusion: Changes in behavior about biosafety and feelings of anxiety and uncertainty about the disease were associated with the dental students' fear of infecting their relatives with COVID-19 as a result of clinical practice. Objective COVID19 COVID 19 COVID-1 practice Methods email e mail family. family data measures Pearsons Pearson s Chisquare Chi square Fishers Fisher p≤0.05. p005 p p≤0.05 . 0 05 (p≤0.05) Results 6 participants 615 61 5 61.5% COVID19, 19, p=0.028 p0028 028 (p=0.028 aerosolgenerating aerosol generating p=0.027, p0027 p=0.027 , 027 (p=0.027) p=0.038, p0038 p=0.038 038 (p=0.038) p=0.047, p0047 p=0.047 047 (p=0.047) postpandemic post p=0.020 p0020 020 (p=0.020 Conclusion COVID1 1 COVID- p00 p≤0.0 (p≤0.05 61.5 p=0.02 p002 02 (p=0.02 (p=0.027 p003 p=0.03 03 (p=0.038 p004 p=0.04 04 (p=0.047 p0 p≤0. (p≤0.0 61. p=0.0 (p=0.0 (p=0.03 (p=0.04 p≤0 (p≤0. p=0. (p=0. p≤ (p≤0 p=0 (p=0 (p≤ p= (p= (p

Abstract Objective: To investigate the effectiveness of linezolid and vancomycin for the treatment of nosocomial infections in children under 12 years old. Data sources: This is a systematic review in which five randomized clinical trials about the effectiveness of linezolid and vancomycin, involving a total of 429 children with nosocomial infections, were evaluated. They were searched in scientific databases: PubMed, Bvs, and SciELO. Summary of findings: The main nosocomial infections that affected children were bacteremia, skin, and soft tissue infections followed by nosocomial pneumonia. Most infections were caused by Gram-positive bacteria, which all studies showed infections caused by Staphylococcus aureus, with methicillin-resistant S. aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci strains being isolated. Both linezolid and vancomycin showed high therapeutic efficacy against different types of nosocomial infections, ranging from 84.4% to 94% for linezolid and 76.9% to 90% for vancomycin. Patients receiving linezolid had lower rates of rash and red man syndrome compared to those receiving vancomycin. However, despite the adverse reactions, antimicrobials can be safely administered to children to treat nosocomial infections caused by resistant Gram-positive bacteria. Conclusion: Both linezolid and vancomycin showed good efficacy in the treatment of bacterial infections caused by resistant Gram-positive bacteria in hospitalized children. However, linezolid stands out regarding its pharmacological safety. Importantly, to strengthen this conclusion, further clinical trials are needed to provide additional evidence. Objective 1 old sources 42 evaluated databases PubMed Bvs SciELO findings bacteremia skin pneumonia Grampositive Gram positive methicillinresistant methicillin S MRSA (MRSA coagulasenegative coagulase negative isolated 844 84 4 84.4 94 769 76 9 76.9 90 However reactions Conclusion safety Importantly conclusion evidence 8 84. 7 76.

Abstract Introduction: contemporary forms of slave labour have different impacts on occupational health, requiring new and emancipatory care practices. Objective: to analyze the theoretical and practical dimensions of the health-disease-care process and workers’ emancipation in a context of enslavement. Methods: this essay problematizes theoretical and conceptual aspects of the health-work-slavery relationship, emphasizing the importance of broadening the concept of worker beyond wage-earning relations. Results: the article presents a critical review of Brazilian experiences in supporting the emancipation of enslaved workers and a critical reading of the ‘National Flow of Assistance to Victims of Slave Labor,’ highlighting the pressing challenge of developing new health care routes and the role of the Brazilian Unified Health System (SUS) and of the workers’ health, especially for post-rescue moments, to overcome the risk of re-enslavement and reduce social vulnerabilities. Conclusion: the continuous struggle to expand and strengthen emancipatory strategies requires the leadership and organisation of workers, connecting unions, social, gender and racial struggles as struggles for freedom and dignity. Fights for health that promote the construction of territories free from slave labor, containing a set of actions to ensure basic conditions for a dignified life and the exercise of freedom. Introduction practices Objective healthdiseasecare disease enslavement Methods healthworkslavery work slavery relationship wageearning wage earning relations Results National Labor, Labor SUS (SUS postrescue post rescue moments reenslavement re vulnerabilities Conclusion unions dignity labor

Resumo Introdução: as formas contemporâneas de trabalho escravo trazem impactos diferenciados à saúde desses trabalhadores, necessitando haver desenvolvimento de práticas de cuidado emancipatório. Objetivo: analisar as dimensões teórico-práticas do processo saúde-doença-cuidado e da emancipação dos(as) trabalhadores(as) em cenário de escravização. Métodos: trata-se de ensaio que problematiza aspectos teórico-conceituais das relações saúde-trabalho-escravidão, destacando a relevância da ampliação da noção de trabalhador para além das relações de assalariamento. Resultados: o artigo apresenta revisão crítica das experiências brasileiras de apoio à emancipação de trabalhador(a)s em situação de escravidão e leitura crítica do “Fluxo nacional de atendimento às vítimas do trabalho escravo”, ressaltando o desafio premente de desenvolver novos caminhos de cuidado em saúde e o papel do Sistema Único de Saúde (SUS) e da saúde do trabalhador, especialmente para os momentos pós-resgate de trabalhadores, de modo a superar o risco de reescravização e reduzir vulnerabilidades sociais. Conclusão: o processo social de luta contínua para ampliar e fortalecer as estratégias emancipatórias requer o protagonismo e organização de trabalhadores, conectando as lutas sindicais, sociais, de gênero e raça como lutas por liberdade e dignidade. Lutas por saúde que promovam a construção de territórios livres de trabalho escravo, contendo conjunto de ações para assegurar condições básicas de vida digna e exercício da liberdade. Introdução trabalhadores emancipatório Objetivo teóricopráticas teórico saúdedoençacuidado doença dosas dos dos(as trabalhadoresas trabalhadores(as escravização Métodos tratase trata se teóricoconceituais conceituais saúdetrabalhoescravidão, saúdetrabalhoescravidão escravidão, saúde-trabalho-escravidão assalariamento Resultados trabalhadoras s Fluxo , escravo” SUS (SUS pósresgate pós resgate sociais Conclusão sindicais dignidade

Abstract Introduction Community health workers, like the physiotherapist, perform essential functions in primary health care, being an important element in the transformation of public policies. There are no reported studies investigating the knowledge of community health workers about health conditions amenable to physiotherapy intervention in primary health care. Objective To construct a questionnaire to investigate the perception of community health workers about health conditions that could be remedied by physiotherapy intervention in primary health care. Methods This was a methodological study in which it was initially an analysis matrix with the aim of encompassing the ideas contemplated in the questionnaire. To construct the instrument, a literature review was carried out, and health conditions treatable with physiotherapy in primary health care were chosen. To validate the content and appearance of the items, twelve physiotherapists specialized in primary health care judged the suitability of the items contained. The content validity index was used to determine the degree of agreement during the response analysis process. Subsequently, a semantic analysis was carried out through the understanding of the items by 15 community health workers. In the validation stage, two rounds of evaluation were carried out. Adjustments were made to 17 questions. Results The study investigated a questionnaire with 20 questions containing hypothetical situations of home visits, in which the resident's situation could or could not constitute a health risk amenable to physiotherapeutic intervention. Conclusion The community health workers perception instrument on health conditions amenable to physiotherapy intervention in primary health care proved to be valid for use in this context. The use of the instrument may contribute to the development of community health worker training programs, with the aim of facilitating team communication. physiotherapist policies chosen contained process Subsequently 1 stage 2 visits residents resident s context programs communication

Resumo Introdução O Agente Comunitário de Saúde (ACS), assim como o fisioterapeuta, desencadeia funções fundamentais na Atenção Primária à Saúde (APS), sendo ele-mento importante na transformação de políticas públicas. Inexistem estudos que investiguem o conhecimento dos ACS sobre as condições de saúde sensíveis à inter-venção da fisioterapia na APS. Objetivo Construir um questionário de investigação da percepção dos ACS sobre as condições de saúde sensíveis à intervenção da fisioterapia na APS. Métodos Trata-se de um estudo metodológico no qual, inicialmente, construiu-se uma matriz de análise com o intuito de englobar as ideias contempladas no questionário. Para a construção do instrumento, realizou-se uma revisão da literatura, sendo eleitas condições de saúde sensíveis à intervenção da fisioterapia na APS. Para a validação de conteúdo e aparente dos itens, 12 fisioterapeutas especialistas em APS julgaram a adequação dos itens contidos. Utilizou-se o índice de validade de conteúdo para verificar o grau de concordância durante o processo de análise das respos-tas. Posteriormente, realizou-se análise semântica por meio da compreensão dos itens por 15 ACS. Na etapa de validação, foram realizadas duas rodadas de avaliação. Foram feitos ajustes em 17 questões. Resultados O estudo resultou em um questionário com 20 questões contendo situações hipotéticas de visitas domiciliares, cuja situação do morador poderia ou não configurar um risco à saúde sensível à intervenção fisioterapêutica. Conclusão O instrumento de percepção dos ACS sobre as condições de saúde sensíveis à intervenção da fisioterapia na APS mostrou-se válido para ser utilizado no contexto da APS. A utilização do instrumento poderá contribuir na elaboração de programas de capacitação dos ACS, com o intuito de facilitar a comunicação da equipe. , (ACS) fisioterapeuta APS, (APS) elemento ele mento públicas inter venção Tratase Trata se qual inicialmente construiuse construiu realizouse realizou literatura 1 contidos Utilizouse Utilizou respostas. respostas respos tas. tas respos-tas Posteriormente avaliação 2 domiciliares fisioterapêutica mostrouse mostrou equipe (ACS (APS

ABSTRACT: This study detected Anaplasma marginale in calvesusing blood smears and nested PCR (nPCR) and to compare the results with the clinical signs presented by calves on a dairy farm in the municipality of Castanhal, located northeast of the state Pará (1°07’19.1”S and 47°53’53.0”W), eastern Amazon. To this end, 192 blood samples were collected from 24 animals at 1-20, 21-41 and 42-60 days of age. Blood smears and nPCR with primers for the msp5 gene were performed. The prevalence of A. marginale was 61.5% (118/192) for the blood smear technique and nPCR (msp5). The manifestation of clinical signs of anaplasmosis also increased significantly over the course of the study (P < 0.0001), being lower in animals aged 1-20 days, but increasing among those aged 21-41 and 42-60 days. These signs were characterized by apathy, fever, weight loss, diarrhea, dehydration, and hypochromic mucous membranes. Regarding the evaluation of the diagnostic techniques, no significant difference was observed in the detection of A. marginale between the blood smear and nPCR (P = 0.995), but the agent’s rickets increased on Day 47 (P < 0.01) in both tests, thereby demonstrating a near-linear pattern of increase in rickets over the 60 days, with a consequent decrease in globular volume. This shows that of the 24 animals studied, 21 were infected at some point during the study period. Additionally, there was no significant difference between blood smears and nPCR, probably due to medium and high parasitemia, which were directly related to the clinical signs and decrease in globular volume. ABSTRACT (nPCR Castanhal 1°0719.1S 107191S S 1°07 19.1 1 07 19 47°5353.0W, 4753530W W 47°53 53.0 , 53 0 47°53’53.0”W) Amazon end 2 120, 120 20, 20 2141 41 21-4 4260 42 42-6 age msp performed A 615 61 5 61.5 118/192 118192 118 (118/192 msp5. . (msp5) P 0.0001, 00001 0.0001 0001 0.0001) 1-2 apathy fever loss diarrhea dehydration membranes techniques 0.995, 0995 0.995 995 0.995) agents agent s 4 0.01 001 01 tests nearlinear near linear 6 volume studied period Additionally parasitemia 0719 1S 107 1°0 191 19. 5353 0W 47°5353.0W 4753 47°5 530 53. 47°53’53.0”W 12 214 21- 426 42- 61. 118/19 11819 11 (118/19 (msp5 0000 0.000 000 1- 099 0.99 99 0.0 00 071 10 1° 535 475 47° 118/1 1181 (118/1 (msp 0.00 09 0.9 9 0. 118/ (118/ (118 (11 (1 (

RESUMO: Objetivou-se, na construção deste trabalho, detectar Anaplasma marginale por meio do esfregaço sanguíneo e Nested PCR (nPCR) e comparar os resultados com os sinais clínicos apresentados pelos bezerros em uma propriedade leiteira localizada no município de Castanhal, região nordeste do estado do Pará (1°07’19,1”S e 47°53’53,0”W), Amazônia Oriental. Para isso, foram coletadas 192 amostras sanguíneas de 24 animais, divididos em três períodos: 1-20, 21-41 e 42-60 dias de idade. Foram realizados esfregaços sanguíneos e nPCR com iniciadores para o gene msp5. A prevalência de A. marginale foi de 61,46% (118/192) tanto para a técnica de esfregaço sanguíneo quanto para nPCR (msp5). A manifestação de sinais clínicos da anaplasmose também foi significativamente crescente ao longo do estudo (P < 0,0001), sendo menor em animais de 1 a 20 dias, mas expandindo-se entre os de 21 a 41 dias e 42 a 60 dias, esses sinais foram caracterizados por apatia, febre, perda de peso, diarreia, desidratação e mucosas hipocoradas. Quanto a avaliação das técnicas diagnósticas, não houve diferença significativa entre a detecção de A. marginale no esfregaço sanguíneo e na nPCR (P = 0,995), porém se observou aumento riquetsêmico do agente no 47º dia (P < 0,01) em ambos os testes, demonstrando, assim, um padrão de aumento da riquetsemia próximo ao linear ao longo dos 60 dias, com consequente diminuição do volume globular. Assim, demonstra-se que dos 24 animais estudados, 21 se infectaram em algum momento do período estudado, e não houve diferença significativa entre esfregaço sanguíneo e nPCR, em virtude, provavelmente, das parasitemias médias e altas, as quais estiveram diretamente relacionadas com os sinais clínicos e a diminuição do volume globular. RESUMO Objetivouse, Objetivouse Objetivou se, Objetivou-se trabalho (nPCR Castanhal 1°0719,1S 107191S S 1°07 19,1 07 19 47°5353,0W, 4753530W W 47°53 53,0 , 47 53 0 47°53’53,0”W) Oriental isso 2 períodos 120, 120 20, 1-20 2141 21-4 4260 42-6 idade msp5 msp 6146 61 46 61,46 118/192 118192 118 (118/192 . (msp5) P 0,0001, 00001 0,0001 0001 0,0001) expandindose expandindo 4 6 apatia febre peso diarreia hipocoradas diagnósticas 0,995, 0995 0,995 995 0,995) º 0,01 001 01 testes demonstrando assim globular Assim demonstrase demonstra estudados estudado virtude provavelmente altas 0719 1S 107 1°0 191 19, 5353 0W 47°5353,0W 4753 47°5 530 53, 5 47°53’53,0”W 12 1-2 214 21- 426 42- 614 61,4 118/19 11819 11 (118/19 (msp5 0000 0,000 000 099 0,99 99 0,0 00 071 10 1° 535 475 47° 1- 61, 118/1 1181 (118/1 (msp 0,00 09 0,9 9 0, 118/ (118/ (118 (11 (1 (

Abstract The article analyzes the federal response of the National Public Health Surveillance and Brazilian Health Regulatory Systems to the Zika virus epidemic in Brazil, from 2015 to 2018, focusing on the political-institutional contexts and the content of government measures developed during the period. The study was anchored in the historical institutionalist approach, comprising documentary analysis and interviews with key actors. The response was characterized by the initial prioritization of the health emergency on the federal government's political agenda, which was reduced over the period, influenced by the financial and political crises. There was a multiplicity of actors and instances focused on contingency, with a certain articulation between the two systems, based on their own pre-emergency experiences. Vector control had centrality, valuing intersectoral and community actions, induced mainly by the Health Surveillance Secretariat of the Ministry of Health and additionally by the National Health Surveillance Agency. Significant allocation limitations of new financial resources and changes in the organizational response apparatus were observed, with effects on the continuity of post-emergency policies, including the development of medicines, vaccines and tests. The Zika Virus Public Health Emergency in Brazil was marked by limited institutionalization of learning and structuring strategies, reducing opportunities for the (re)organization of surveillance in the Unified Health System. 201 2018 politicalinstitutional institutional period approach governments s agenda crises contingency systems preemergency pre experiences centrality actions Agency observed postemergency post policies medicines tests strategies reorganization re organization System 20 2

Resumo O artigo analisa a resposta federal dos Sistemas Nacionais de Vigilância em Saúde e de Vigilância Sanitária frente à epidemia do Zika vírus no Brasil, de 2015 a 2018, com foco nos contextos político-institucionais e no conteúdo das medidas governamentais desenvolvidas no período. O estudo ancorou-se na abordagem institucionalista histórica, compreendendo análise documental e entrevistas com atores-chave. A resposta se caracterizou pela priorização inicial da emergência sanitária na agenda política do governo federal, reduzindo-se ao longo do período, influenciada pelas crises financeira e política. Verificou-se multiplicidade de atores e instâncias voltadas à contingência, com certa articulação entre os dois sistemas, a partir de experiências pré-emergência próprias. O controle vetorial teve centralidade, valorizando ações intersetoriais e comunitárias, induzidas sobretudo pela Secretaria de Vigilância em Saúde do Ministério da Saúde e complementarmente pela Agência Nacional de Vigilância Sanitária. Observaram-se expressivas limitações alocativas de recursos financeiros novos e mudanças no aparato organizativo de resposta, com efeitos para a continuidade das políticas no pós-emergência, incluindo o desenvolvimento de medicamentos, vacinas e testes. A Emergência em Saúde Pública do Zika Vírus no Brasil foi marcada por limitada institucionalização de aprendizados e estratégias estruturantes, reduzindo oportunidades para a (re)organização das vigilâncias no Sistema Único de Saúde. 201 2018 políticoinstitucionais político institucionais período ancorouse ancorou histórica atoreschave. atoreschave chave. chave atores-chave reduzindose Verificouse Verificou contingência sistemas préemergência pré próprias centralidade comunitárias Observaramse Observaram pósemergência, pósemergência pós emergência, pós-emergência medicamentos testes estruturantes reorganização re organização 20 2

Abstract Objective To review key evidence-based recommendations for the diagnosis and treatment of peripheral facial palsy in children and adults. Methods Task force members were educated on knowledge synthesis methods, including electronic database search, review and selection of relevant citations, and critical appraisal of selected studies. Articles written in English or Portuguese on peripheral facial palsy were eligible for inclusion. The American College of Physicians’ guideline grading system and the American Thyroid Association’s guideline criteria were used for critical appraisal of evidence and recommendations for therapeutic interventions. Results The topics were divided into 2 main parts: (1) Evaluation and diagnosis of facial palsy: electrophysiologic tests, idiopathic facial palsy, Ramsay Hunt syndrome, traumatic peripheral facial palsy, recurrent peripheral facial palsy, facial nerve tumors, and peripheral facial palsy in children; and (2) Rehabilitation procedures: surgical decompression of the facial nerve, facial nerve grafting, surgical treatment of long-term peripheral facial palsy, and non-surgical rehabilitation of the facial nerve. Conclusions Peripheral facial palsy is a condition of diverse etiology. Treatment should be individualized according to the cause of facial nerve dysfunction, but the literature presents better evidence-based recommendations for systemic corticosteroid therapy. evidencebased based adults methods search citations studies inclusion Physicians Associations Association s interventions parts 1 (1 tests syndrome tumors (2 procedures grafting longterm long term nonsurgical non etiology dysfunction therapy (

ABSTRACT: Seafood is one of the most important sources of nutrients. However, they have a short shelf-life and the traditional preservation methods may generate losses in their natural flavour and nutrients. Thisstudy evaluated and optimize the High Pressure Processing (HPP) regarding pressure level (200-400 MPa) and holding time (0-5 min) applied to lion’s paw scallop (Nodipecten nodosus) to reduce microbial contamination while maintaining desirable characteristics. Response surface methodology with a Box-Behnken design and Desirability function were employed to simultaneously optimize these quality attributes. HPP enhanced microbial quality at 200 MPa/5 min, despite promoting inadequate physical-chemical modifications in the adductor muscle of the scallop. In such processing condition, in spite of a slight increase in muscle humidity which could be of benefit, pH increase was also verified, as well as a decrease in water holding capacity (WHC). At more severe level (400 MPa/5 min), a decrease in the shear force related to instrumental texture and in Whiteness (W) and Luminosity (L*) related to color was observed. Simultaneous optimization provided a value of 365MPa / 2min where physicochemical characteristics would be the more similar to the scallop without facing a preservation process. ABSTRACT nutrients However shelflife shelf life (HPP 200400 400 (200-40 MPa 05 0 5 (0- min lions lion s Nodipecten nodosus BoxBehnken Box Behnken attributes 20 MPa5 MPa/ physicalchemical physical chemical condition benefit verified WHC. WHC . (WHC) (40 , W (W L* L (L* observed process 20040 40 (200-4 (0 2 (WHC (4 (L 2004 4 (200- ( (200 (20 (2

RESUMO: Os frutos do mar são uma das fontes mais importantes de nutrientes. No entanto, possuem vida de prateleira curta e os métodos tradicionais de conservação podem gerar perdas em seu sabor natural e nutrientes. O objetivo deste estudo foi avaliar e otimizar o Processamento por Alta Pressão (APH) em relação ao nível de pressão (200-400 MPa) e tempo de espera (0-5 min) aplicado à vieira pata de leão (Nodipecten nodosus) para reduzir a contaminação microbiana, mantendo características desejáveis. Metodologia de superfície de resposta com design Box-Behnken e função de Desejabilidade foram empregadas para otimizar simultaneamente esses atributos de qualidade. A APH melhorou a qualidade microbiana a 200 MPa/5 min, apesar de promover modificações físico-químicas inadequadas no músculo adutor da vieira. Nessa condição de processamento, apesar de um leve aumento da umidade do músculo que poderia ser benéfico, também foi verificado aumento do pH, bem como diminuição da capacidade de retenção de água (CRA). No nível mais severo (400 MPa/5 min), observou-se uma diminuição na força de cisalhamento relacionada à textura instrumental e na Brancura (W) e Luminosidade (L*) relacionada à cor. A otimização simultânea proporcionou um valor de 365MPa/2min em que as características físico-químicas seriam as mais semelhantes às da vieira sem enfrentar um processo de preservação. RESUMO nutrientes entanto (APH 200400 400 (200-40 MPa 05 0 5 (0- min Nodipecten nodosus desejáveis BoxBehnken Box Behnken 20 MPa5 MPa/ físicoquímicas físico químicas processamento benéfico pH CRA. CRA . (CRA) (40 , observouse observou se W (W L* L (L* cor 365MPa2min MPamin 365MPa 2min preservação 20040 40 (200-4 (0 2 (CRA (4 (L 2004 4 (200- ( (200 (20 (2

Abstract Pitaya production has been expanding in several regions of Brazil despite the lack of technical information on plant management. To obtain good crop productivity, weed control is essential; however, the use of herbicides can cause phytotoxicity and reduce plant growth and production. Thus, the present work aimed to evaluate the tolerance of red (Selenicereus polyrhizus) and white (S. undatus) pitaya seedlings to different herbicides applied after emergence. Two experiments were installed, one with red pitaya and the another with white pitaya, on August 15, 2020 in the Fruit Production Sector of the Federal University of Lavras. Experiments were arranged in a randomized block design, with four and three replicates, respectively, eight treatments and three plants per plot in both experiments. Treatments consisted of control, fomesafen, ammonium glufosinate, glyphosate, clethodim, carfentrazone-ethyl, imazethapyr and chlorimuron. Visual phytotoxicity symptoms were evaluated at 7, 14, 21 and 28 days after herbicide application. At 30 and 60 days after application, percentage of necrosis, cladode length and number of new shoots were evaluated. Herbicide fomesafen caused greater damage to red pitaya seedlings and fomesafen and glyphosate caused greater damage to white pitaya seedlings. For both pitaya species, herbicide clethodim can be an alternative for controlling narrow-leaf weeds without causing visual damage to the growth and development of pitaya plants. management productivity essential however Thus Selenicereus polyrhizus S. S (S undatus emergence installed 15 202 Lavras design replicates respectively glufosinate carfentrazoneethyl, carfentrazoneethyl carfentrazone ethyl, ethyl carfentrazone-ethyl chlorimuron 7 14 2 application 3 6 necrosis species narrowleaf narrow leaf 1 20

Resumo A produção de pitaia vem-se expandindo em várias regiões do Brasil apesar da carência de informações técnicas sobre o manejo das plantas. Para obter boa produtividade na cultura, o controle de plantas daninhas é fundamental; porém, o uso de herbicidas pode causar fitotoxicidade e redução do crescimento e da produção das plantas. Assim, o presente trabalho teve como objetivo avaliar atolerância de mudas de pitaia de polpa vermelha (Selenicereus polyrhizus) e polpa branca (S.undatus) a diferentes herbicidas aplicados em pós-emergência. Foram instalados doisexperimentos: um com pitaia-vermelha e outro com pitaia-branca, em 15 de agosto de 2020, no Setor de Fruticultura da Universidade Federal de Lavras. Os experimentos foramdispostos em delineamento em blocos casualizados, com quatro e três repetições, respectivamente, oito tratamentos e três plantas por parcela, em ambos os experimentos. Ostratamentos foram constituídos por testemunha, fomesafem, glufosinato de amônio, glifosato,cletodim, carfentrazona-etílica, imazetapir e clorimurom. Foram avaliados sintomas visuais defitotoxicidade aos 7; 14; 21 e 28 dias após a aplicação dos herbicidas. Aos 30 e 60 dias após a aplicação,avaliaram-se a porcentagem de necrose, comprimento dos cladódios e o número de brotos novos. O herbicida fomesafem causou maiores danos às mudas depitaia-vermelha,e fomesafem e glifosato causaram maiores danos às mudas de pitaia-branca. Paraambas as espécies de pitaias, o herbicida cletodim pode ser uma alternativa paracontrole de plantas daninhas de folha estreita sem provocar danos visuais no crescimento e nodesenvolvimento das plantas de pitaia. vemse vem se cultura fundamental porém Assim Selenicereus polyrhizus S.undatus Sundatus S undatus (S.undatus pósemergência. pósemergência pós emergência. emergência pós-emergência doisexperimentos pitaiavermelha pitaiabranca, pitaiabranca branca, pitaia-branca 1 2020 Lavras casualizados repetições respectivamente parcela testemunha amônio glifosatocletodim glifosato,cletodim carfentrazonaetílica, carfentrazonaetílica carfentrazona etílica, etílica carfentrazona-etílica clorimurom 7 14 2 3 6 aplicação,avaliaramse aplicaçãoavaliaramse aplicação,avaliaram avaliaram necrose novos depitaiavermelha,e depitaiavermelhae depitaia vermelha,e pitaiabranca. branca. pitaias 202 avaliaramse aplicaçãoavaliaram depitaiavermelha vermelhae 20

Abstract This study aims to investigate the gene expression of sperm-borne phospholipase C zeta (PLCζ), WW domain-binding protein 2N-Terminal Like (WBP2NL), and Tumor necrosis factor (TNF-α), as a negative control, in spermatozoa and their relationship with fertility and seminal quality in stallions. Ejaculates from 40 Criollo stallions were used, whose fertility was assessed on the basis of their pregnancy rate per cycle in at least two breeding seasons. Pregnancy rates ranged from 20% to 90% and were used to divide the stallions into two groups: High rates (≥ 50%) (n = 25), and Low rates (< 50%) (n = 15). A computer-assisted sperm analysis system - (CASA) analyzed semen after collection. Also were evaluated the physical and functional integrity of the plasmatic membrane and sperm morphology alterations. All stallions expressed PLCζ, WBP2NL, and TNF-α. PLCζ positively correlates with conception rate, total motility (TM), progressive motility (PM), plasmatic membrane functionality, and integrity. A simple linear regression was detected between pregnancy rate and PLCζ expression (P = 0.003), TM (P < 0.001) and PM (P < 0.001). PLCζ gene expression was higher (P = 0,012) in the High rates group than in the Low group. WBP2NL and TNF-α did not correlate with seminal quality and stallion’s fertility. It was concluded that PLCζ gene expression in the spermatozoa might be used as a biomarker of fertility and seminal quality in stallions. Parameters of sperm kinetics also showed, positive correlation between TM, PM and pregnancy rate. spermborne borne , (PLCζ) domainbinding domain binding 2NTerminal NTerminal 2N Terminal N WBPNL WBP NL (WBP2NL) TNFα, TNFα TNF α (TNF-α) control 4 seasons 20 90 groups ≥ ( 50% 50 n 25, 25 25) 15. 15 . 15) computerassisted computer assisted CASA (CASA collection alterations TNFα. α. (TM) PM, (PM) functionality P 0.003, 0003 0.003 0 003 0.003) 0.001 0001 001 0.001. 0,012 0012 012 stallion s showed (PLCζ (WBP2NL (TNF-α 2 9 5 1 (TM (PM 000 0.00 00 0,01 01 0.0 0,0 0. 0,

Resumen: Este artículo aborda la relación entre la subjetividad y el trabajo esclavo contemporáneo desde la narrativa de trabajadores esclavizados. Se realizó una investigación social cualitativa desde una perspectiva construccionista. Buscamos la interacción con los trabajadores liberados y utilizamos la observación participante de la rutina de los trabajadores atendidos en un proyecto institucional, diario de campo y entrevistas individuales (semiestructuradas y abiertas) con trabajadores y miembros del equipo del proyecto institucional. El trabajo de campo duró un año y medio, y se utilizó como apoyo el análisis de contenido. Para la interpretación se utilizaron aportes de la teoría freudiana y el pensamiento de Foucault, con lo que fue posible comprender aspectos de las vivencias de los trabajadores, características del escenario de explotación, abandono parental y las tensiones relacionadas con la autoclasificación “esclavo”. Las narrativas apuntan a una realidad dramática manifestada en la explotación del cuerpo, abuso autoritario, violencia y abandono. Al mismo tiempo, evidenciaron formas de resistencia por parte de los trabajadores, que reclaman más investigaciones para profundizar en el conocimiento sobre las vivencias subjetivas de quienes se encuentran en esclavitud. Resumen esclavizados construccionista institucional semiestructuradas abiertas medio contenido Foucault esclavo. . “esclavo” cuerpo autoritario tiempo esclavitud “esclavo

Abstract: This study addresses the relation between subjectivity and contemporary enslaved labor from the enslaved workers’ narratives in Brazil. A qualitative social research was carried out based on a constructionist perspective. We sought interaction with rescued workers and used (a) participant observation of workers’ routine in an institutional project that supports them and a field diary, (b) semi-structured and open individual interviews with workers and a member of the team project. The fieldwork lasted a year and a half and the analysis followed Content Analysis. Freudian theory and Foucault’s thought were used for interpretation, which managed to understand aspects of workers’ experiences, exploitation characteristics, parental abandonment, as well as the tensions in self-classification as enslaved. The narratives pointed to a dramatic reality manifested in body exploitation, authoritarian abuses, violence, and negligence. At the same time, these narratives showed forms of worker resistance that calls for further investigations to increase knowledge on the subjective experiences of those who were enslaved. Abstract Brazil perspective (a diary b (b semistructured semi structured Analysis Foucaults Foucault s interpretation characteristics abandonment selfclassification self classification abuses violence negligence time

Resumo: O artigo aborda as relações entre subjetividade e trabalho escravo contemporâneo a partir da narrativa de trabalhadores(as) escravazados(as). Foi realizada uma pesquisa social qualitativa em uma perspectiva construcionista. Buscamos a interação com trabalhadores resgatados e realizamos a observação participante da rotina de trabalhadores atendidos em um projeto institucional, com diário de campo, e entrevistas individuais (semiestruturadas e abertas) com trabalhadores(as) e equipe do projeto institucional. O trabalho de campo durou um ano e meio, e a pesquisa foi realizada com o suporte da Análise de Conteúdo. Para a interpretação utilizamos aportes da teoria freudiana e do pensamento de Foucault, com os quais foi possível compreender aspectos das vivências dos trabalhadores, características da exploração, abandonos parentais e tensões em torno da autoclassificação como “escravo”. As narrativas apontaram uma realidade dramática manifesta na exploração do corpo, em abusos autoritários, na violência e negligência. Ao mesmo tempo, as narrativas evidenciaram formas de resistência dos trabalhadores que convocam mais investigações para adensar o conhecimento sobre as experiências subjetivas desses que estão num lugar de escravizado(a). Resumo trabalhadoresas trabalhadores(as escravazadosas. escravazadosas escravazados . escravazados(as) construcionista institucional semiestruturadas abertas meio Conteúdo Foucault escravo. “escravo” corpo autoritários negligência tempo escravizadoa. escravizadoa escravizado escravizado(a) escravazados(as “escravo escravizado(a

The compound 2-hydrazinyl-4-(4-methoxyphenyl)thiazole (PD76) is a novel thiazolyl hydrazine derivative with proven antifungal activity against different fungal species, mainly Candida and Cryptococcus. Considering the advantages of oral route for clinical therapy, the aim of this work was to evaluate the potential intestinal permeability of this new antifungal drug. For the quantitation of PD76, a high-performance liquid chromatography method was developed and fully validated. The cytotoxicity of the compound in Caco-2 cells was analyzed and intestinal permeability of PD76 was assessed by means of the comparison of in vitro assay in Caco-2 cells and in silico platforms ADMETlab and admetSAR. Cell viability above 70% was obtained at all PD76 studied concentrations. Using Caco-2 cell model, the compound showed apparent permeability coefficients (Papp) of 5.25 × 10-6 and 23.28 × 10-6 cm s-1 in apical-basolateral and basolateral-apical directions, respectively. Experiments performed using verapamil as P-gp inhibitor demonstrated that PD76 is slightly susceptible to active efflux. Both in silico platforms inferred that PD76 presents permeability in Caco-2 cells, with Log P values of 2.82 (ADMETlab) and 2.10 (admetSAR). The results obtained in permeability studies showed that PD76 presents moderate intestinal permeability and a promising profile for clinical application. 2hydrazinyl44methoxyphenylthiazole hydrazinylmethoxyphenylthiazole 2 hydrazinyl 4 methoxyphenyl thiazole PD (PD76 species Cryptococcus therapy drug highperformance high performance validated Caco2 Caco Caco- PD7 admetSAR 70 concentrations model Papp (Papp 525 5 25 5.2 106 10 6 10- 2328 23 28 23.2 s1 s 1 s- apicalbasolateral apical basolateral basolateralapical directions respectively Pgp gp efflux 282 82 2.8 (ADMETlab 210 2.1 . (admetSAR) application methoxyphenylthiazole (PD7 7 52 5. 232 23. 8 2. 21 (admetSAR (PD