ABSTRACT The Scientists of Tomorrow/ Cientistas do Amanhã project is an immersive science training program developed by the Program of Post-Graduation in Health Sciences at Hospital Israelita Albert Einstein. This program was conducted in partnership with Volunteering and Escola Municipal de Ensino Fundamental Professor Paulo Freire in Paraisópolis, São Paulo, Brazil. The Scientists of Tomorrow Program comprised a short training period conducted in May 2022 involving 37 students, and a long training period from August to December 2022, which included 15 students. It aimed to popularize science through practical activities; transfer knowledge to young students; sensitize and guide them to pursue academic-scientific careers; reduce stereotypes about scientific work and scientists; and help students understand the social, political, and ethical roles of science within society. All activities were led by postgraduate students and professors from our postgraduate program, physicians, nurses, physiotherapists, biomedicals, and veterinarians from Hospital Israelita Albert Einstein, as well as medical students from Faculdade Israelita de Ciências da Saúde Albert Einstein . Activities in the short training included lectures on cinema and science, strategies to combat fake news, non-violent communication, innovation, design-thinking framework, and developing a scientific project. During the long training period, discussions were focused on nanotechnology, animal research, big data, bioinformatics, meditation, blood and bone marrow donation, telemedicine, sex and sexually-transmitted infections, rehabilitation, career opportunities, and scientific integrity. In addition, practical activities were further expanded using optical and confocal microscopy, cytometry, and basic concepts regarding the structure and function of living cells. The program also included the launching of the open-air outreach Education E-natureza activity, which turned students into ambassadors of nature. In conclusion, the Scientists of Tomorrow Program was innovative and enabled young students to learn that science is a collective activity that can enhance public health. PostGraduation Post Graduation Paraisópolis Brazil 202 3 1 academicscientific academic careers scientists social political society physicians nurses physiotherapists biomedicals news nonviolent non violent communication innovation designthinking design thinking framework nanotechnology research data bioinformatics meditation donation telemedicine sexuallytransmitted sexually transmitted infections rehabilitation opportunities integrity addition microscopy cytometry cells openair open air Enatureza E natureza nature conclusion health 20 2

Abstract This study aimed to evaluate the application of good practices in hotel restaurants in Southern Brazil, relate these practices to the microbiological quality of the food offered, in addition to identifying contamination sources. The food and beverage sector of four hotels was evaluated by means of a checklist based on the Resolution 216/2014 of Agência Nacional de Vigilância Sanitária and microbiological analyzes. Surface, utensil, equipment and handlers’ hand samples were collected for coagulase positive Staphylococcus and thermotolerant coliform count. Isolates obtained from these culture counts were compared by band profiles obtained by rep-PCR. According to non-conformities observed, only one hotel was rated as excellent in good practices avaliation. Some microbiological analysis exceeded the allowed limit for the analyzed microorganism count, revealing failure in the hygiene-sanitary process. Genetic similarities between some food samples and handlers’ hands and utensils were identified. These results showed inadequacies in the process of food care and handling which may compromise the quality of food offered to custumers.

Resumo Objetivo: Avaliou-se o efeito dos fatores ambientais sobre a estrutura da comunidade de ostrácodes perifíticos ao longo do sistema rio-planície de inundação do alto rio Paraná. Foi predito que quanto maior a distância a jusante da barragem de Porto Primavera, maior a diversidade e densidade de ostrácodes. Métodos Ostrácodes perifíticos (associados aos sistemas radiculares da macrófita flutuante Eichhornia spp) foram amostrados durante novembro de 2013 a maio de 2015. Três amostras foram coletadas em cada ponto de amostragem (canal principal do rio Paraná, tributários e lagoas). Plantas de Eichhornia foram removidas da água manualmente e colocadas em balde plástico. As raízes foram lavadas para a remoção dos ostrácodes, e as amostras foram filtradas através de uma rede de mão de 160 µm de abertura de malha. Resultados O levantamento faunístico registrou 44 espécies de ostrácodes e os estimadores de riqueza indicaram que o esforço amostral pareceu ser adequado para revelar a diversidade de ostrácodes nesta área de estudo. A maior riqueza e densidade foram observadas no trecho a jusante da barragem no canal principal do rio Paraná e nas lagoas. A diversidade beta não foi significativamente diferente ao longo do gradiente longitudinal a jusante e a maior similaridade na composição de espécies de ostrácodes foi observada nas lagoas localizadas no trecho a jusante da barragem. Conclusões Um aumento na riqueza e densidade de ostrácodes foram registrados nas seções do rio Paraná, a jusante da barragem, evidenciando a importância dos tributários não represados sobre alguns atributos da comunidade de ostrácodes. A similaridade da diversidade beta entre os pontos de amostragem pode ser devido à permanente conectividade hidrológica entre estes ambientes, favorecendo a troca de organismos principalmente através da deriva de macrófitas flutuantes livres, e devido a dispersão passiva dos ovos de ostrácodes resistentes a seca. Os fatores abióticos locais tiveram efeitos significativos sobre a abundância e distribuição de algumas espécies de ostrácodes.

Abstract Aim: We assessed the effect of environmental factors on the structure of periphytic ostracods communities along the river-floodplain system of the Upper Paraná River. We predict that the higher distance from Porto Primavera dam downstream would lead to higher diversity and density of the ostracods. Methods Periphytic ostracods (associated with root systems of the floating Eichhornia spp) were sampled during November 2013 to May 2015. Three samples were collected at each sampling site (channel of the Paraná River, tributaries and lakes). Eichhornia plants were removed from the water by hand and the plants were placed in a plastic bucket. Roots were washed for the removal of ostracods, and samples were filtered through a hand net with 160 µm mesh size. Results The faunistic survey recorded 44 ostracods species and richness estimators indicated that sampling effort appears to be suitable to reveal the diversity of ostracods in this studied area. The highest richness and density were observed in the stretch downstream of the dam in the main channel of Paraná River and in the lakes. Beta diversity was not significantly different along the longitudinal gradient downstream and higher similarity of ostracods species composition was observed in the lakes located in the stretch downstream of the dam. Conclusions An increase in the richness and density of ostracods were recorded in the sections downstream of the Paraná River, evidencing the importance of undammed tributaries on the ostracods community. The similarity of beta diversity amongst sampling sites may be owing to permanent hydrological connectivity amongst these environments, favouring the exchange of organisms mainly through the drift of free-floating macrophytes, and owing to the passive dispersal of drought resistant eggs of ostracods. Local abiotic factors had significant effects on abundance and distribution of some ostracod species.