Abstract Objective The association between Papillary Thyroid Carcinoma (PTC) and coexistent Hashimoto’s Thyroiditis (HT) was controversial. The purpose of this study was to evaluate the presence of HT exerts any influence on the aggressiveness of PTC, and to establish a nomogram for predicting the possibility of aggressiveness in PTC. Methods 373 consecutive PTC patients with/without coexistent HT from January 2017 to December 2020 were retrospective reviewed. Patients’ clinicopathologic and sonographic characteristics were collected for univariate and multivariate analyses. A nomogram was established based on the risk factors for aggressiveness in PTC. Results Male (p= 0.001), tumor size >1.0 cm (p= 0.046) and lymph node metastasis (p= 0.018) were negatively associated with PTC coexisted with HT, while it was significantly positively associated with the frequence of multifocality (p= 0.010). Univariate and multivariate analyses suggested that age ≥55 years (p= 0.000), male (p= 0.027), HT (p= 0.017), tumor size >1.0 cm (p= 0.015), multifocality (p= 0.041), distance to capsular ≤0 cm (p= 0.050) and blood flow (Grade I: p= 0.044) were independent risk factors for predicting the aggressiveness in PTC. A nomogram according to these predictors was further developed and validated. The receiver operating characteristic curve (AUC = 0.734 and 0.809 for training and validation cohorts, respectively) and decision curve analyses indicated that the nomogram model was clinically useful. The calibration curve revealed that the nomogram exhibited an excellent consistency. Conclusions In this study, the coexistent HT might play a protective role in preventing the proliferation of PTC. Dispensable aggressive treatment may be reduced in PTC by pre-operative identification of sonographic and clinical characteristics and incorporating with the predicted nomogram model. (PTC Hashimotos Hashimoto s (HT controversial 37 withwithout without 201 202 reviewed Patients p (p 0.001, 0001 0.001 , 0 001 0.001) 10 1 >1. 0.046 0046 046 0.018 0018 018 0.010. 0010 0.010 . 010 0.010) 55 ≥5 0.000, 0000 0.000 000 0.000) 0.027, 0027 0.027 027 0.027) 0.017, 0017 0.017 017 0.017) 0.015, 0015 0.015 015 0.015) 0.041, 0041 0.041 041 0.041) ≤ 0.050 0050 050 Grade I 0.044 0044 044 validated AUC 0734 734 0.73 0809 809 0.80 cohorts respectively useful consistency preoperative pre operative 3 20 0.00 00 >1 0.04 004 04 0.01 01 5 ≥ 002 0.02 02 0.05 005 05 073 73 0.7 080 80 0.8 2 0.0 > 07 7 0. 08 8

There are few methods to obtain uniform and well dispersed cuprous oxide nanoparticles, which limits its application widely. In this research, an improved method to prepare nanoparticles was introduced. Cuprous oxides were prepared by the reduction of cupric nitrate, with hydrazine hydrate as a reductant in the presence of glucose. The nanoparticles formed have the characteristics of small particle size and uniform distribution. The crystalline structure and morphological characteristics were analyzed by XRD and TEM respectively. The result showed they were pure sphere polycrystal. The particle size is about 9nm. Subsequently, the antifouling activity was tested in detail through comparative experiments. The results indicated that the effective concentration of nanoparticles was obviously lower than ordinary cuprous oxide.

In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce osteoblasts, chondrocytes and adipocytes. MSCs that differentiated into adipocytes were stimulated with and without bFGF. The OD/DNA (optical density/content of total DNA) and expression levels of the specific adipocyte genes PPARγ2 (peroxisome proliferator activated receptor γ2) and C/EBPs were higher in bFGF cells. Embryonic bodies had a higher adipocyte level compared with cells cultured in plates. These findings indicate that bFGF promotes adipocyte differentiation. MSCs may be useful cells for seeding in tissue engineering and have enormous therapeutic potential for adipose tissue engineering.