Abstract Gastric cancer (GC) often develops resistance to cisplatin treatment, but while latent transforming growth factor β-binding protein (LTBP2) is recognized as a potential regulator in GC, its specific role in cisplatin resistance is not fully understood. This study investigated LTBP2’s impact on cisplatin resistance in GC. LTBP2 expression was assessed in various GC cell lines, and its correlation with cisplatin sensitivity was determined through cell viability assays. Lentivirus-mediated LTBP2 silencing in HGC-27 cells demonstrated enhanced cisplatin sensitivity, reduced cell proliferation, and inhibition of the NF-κB2/Bcl-3/cyclin D1 pathway. Additionally, transient transfection overexpressed the NFκB2 gene in LTBP2-silenced HGC-27/DDPR cells, restoring cisplatin sensitivity and upregulating p52/Bcl-3/cyclin D1. In conclusion, silencing LTBP2 could effectively inhibit cell proliferation and mitigate cisplatin resistance via the NFKB noncanonical pathway NFKB2 p52/Bcl-3/cyclin D1. These findings propose LTBP2 as a potential therapeutic target for overcoming cisplatin resistance in GC patients. (GC treatment βbinding β binding LTBP (LTBP2 understood LTBP2s LTBPs s lines assays Lentivirusmediated Lentivirus mediated HGC27 HGC 27 HGC-2 NFκB2/Bcl3/cyclin NFκB2Bcl3cyclin NFκBBclcyclin NF κB2/Bcl 3/cyclin κB2 Bcl 3 cyclin κB D Additionally NFκB LTBP2silenced LTBPsilenced silenced HGC27/DDPR HGC27DDPR HGCDDPR 27/DDPR DDPR p52/Bcl3/cyclin p52Bcl3cyclin pBclcyclin p52/Bcl p52 p conclusion patients (LTBP HGC2 2 HGC- Bcl3 κB2Bcl κBBcl 3cyclin 27DDPR p52Bcl pBcl p5

Abstract Objectives The aim of this study was to examine the changes in gray matter in nasopharyngeal carcinoma patients with normal hearing (Group 1) and nasopharyngeal carcinoma patients with hearing loss (Group 2) after radiotherapy using voxel-based morphological analysis and to analyze the relationship with the radiation doses of the temporal lobe. Methods 21 patients in Group 1, 14 patients in Group 2, and 21 healthy volunteers were selected. All participants underwent an otologic examination and three-dimensional magnetization preparatory rapid acquisition gradient echo sequence scan. The correlation between the variation of whole brain gray matter volume and the doses of the temporal lobe was analyzed by Data Processing & Analysis for Brain Imaging software. Results Compared with the normal control group, the brain areas with reduced gray matter volume in nasopharyngeal carcinoma patients after radiotherapy were mainly in the left posterior cerebellar lobe (T = −8.797), left insular lobe (T = −7.96), and the right insular lobe (T = −6.632). Compared to Group 1, the brain areas of Group 2 patients with reduced gray matter volume were mainly in the left superior temporal gyrus (T = −2.366), left olfactory bulb (T = −2.52), left Rolandic operculum (T = −2.431), and right olfactory bulb (T = −3.100). Compared with Group 1, the brain areas of Group 2 patients with increased gray matter volume were mainly in the left calcarine sulcus (T = 3.425) and right calcarine sulcus (T = 3.169). There were no correlations between the changes of brain gray matter volume and the radiation doses of the temporal lobe in both Group 1 and Group 2. Conclusions The radiotherapy may cause the changes of brain areas associated with cognitive function in nasopharyngeal carcinoma in a long-term follow-up. At the same time, nasopharyngeal carcinoma patients with the radiation-induced hearing loss had abnormal gray matter volumes in the auditory center and other sensory centers. Our findings might provide new understanding into the pathogenesis of radiation-induced brain damage in normal-appearing brain tissue. Yet this exploratory study should be taken with caution. voxelbased voxel based selected threedimensional three dimensional scan software group T −8.797, 8797 −8.797 , 8 797 −8.797) −7.96, 796 −7.96 7 96 −7.96) −6.632. 6632 −6.632 . 6 632 −6.632) −2.366, 2366 −2.366 366 −2.366) −2.52, 252 −2.52 52 −2.52) −2.431, 2431 −2.431 431 −2.431) −3.100. 3100 −3.100 3 100 −3.100) 3.425 3425 425 3.169. 3169 3.169 169 3.169) longterm long term followup. followup follow up. up follow-up time radiationinduced induced centers normalappearing appearing tissue caution 879 −8.79 79 −7.9 9 663 −6.63 63 236 −2.36 36 25 −2.5 5 243 −2.43 43 310 −3.10 10 3.42 342 42 316 3.16 16 87 −8.7 −7. 66 −6.6 23 −2.3 −2. 24 −2.4 4 31 −3.1 3.4 34 3.1 −8. −7 −6. −2 −3. 3. −8 − −6 −3

RESUMEN Objetivos: Este estudio tuvo como objetivo examinar la relación entre el salto vertical y la fuerza en tiempo específico y el desempeño del sprint en velocistas adolescentes. Métodos: Participaron en el estudio quince adolescentes varones velocistas (edades: 14 ± 2 años, estatura: 168 ± 2 cm, peso: 61 ± 1 kg). Los individuos realizaron los siguientes saltos bilaterales y unilaterales en una plataforma de fuerza: a) squat jump (SJ), b) SJ unilateral (USJ), c) drop jump (DJ) de 40 cm e d) DJ unilateral (UDJ) de 20 cm. La prueba de sprint de 60 m se realizó el segundo día. Los cronómetros en el entrenamiento fraccionado se ajustaron para registrar tiempos de 5 m, 10 m, 50 m y 60 m. Las variables que se incluyeron fueron la altura del salto vertical, la fuerza máxima y la salida de fuerza a 120 m en todos los saltos y mediciones del tiempo del sprint. Resultados: Los resultados del análisis de correlación producto-tiempo de Pearson revelaron que el SJ de 120 m estaba correlacionado con 5 m y el USJ de 120 m estaba correlacionado con 10 m. El UDJ de 120 m tuvo una mayor correlación con el DJ de 50 m que con el de 120 m. Aunque se observaron correlaciones significativas con la fuerza y la altura máximas, algunos resultados fueron inconsistentes entre los saltos bilaterales y unilaterales. Conclusiones: Nuestros resultados pusieron de manifiesto que los saltos con una forma similar a determinadas salidas de fuerza en un tiempo específico del evento pueden predecir con mayor precisión el desempeño en el sprint en adolescentes velocistas. El USJ de 120 m y el UDJ de 120 m pueden predecir mejor, respectivamente, la aceleración (10 m) y la fase de alta velocidad (50 m) en el desempeño del sprint. Además, los entrenadores y practicantes deben ser cautelosos a la hora de utilizar únicamente la altura del salto o la fuerza máxima para predecir el desempeño en el sprint, ya que los resultados pueden ser inexactos cuando no se tienen en cuenta con precisión las variables específicas del movimiento. Nivel de evidencia III. Objetivos Métodos edades (edades años estatura 16 peso 6 kg. kg . kg) SJ, , (SJ) b USJ, (USJ) c (DJ 4 d (UDJ día 12 Resultados productotiempo producto máximas Conclusiones mejor respectivamente (1 (5 Además movimiento III (SJ (USJ (

ABSTRACT Objectives: This study aimed to examine the relationship between vertical jumping at forces of specific time phase and sprint performance in teenage sprinters. Methods: Fifteen male teenage sprinters (age: 14±2 years, height: 168±2 cm, weight: 61±1 kg) participated in the study. The subjects performed the following bilateral/unilateral jumps on a force platform: a) squat jump (SJ), b) unilateral SJ (USJ), c) 40cm drop jump (DJ), and d) 20cm unilateral DJ (UDJ). The 60m sprint test was administered on the second day. Brower split timers were positioned to record subjects’ 5m, 10m, 50m and 60m split times. The variables for inclusion were vertical jump height, maximum force, and force output at 120ms in all jumps and sprint time measures. Results: The results of the Pearson Product Moment Correlation analysis showed that SJ120ms was correlated to 5m and USJ120ms was correlated to 10m. UDJ120ms showed a stronger correlation with 50m than DJ120ms. Although significant correlations using maximum force and height were observed, there were inconsistent results between bilateral and unilateral jumps. Conclusion: Our results highlighted that jumps that have similar form with certain force outputs at specific event timing could more precisely predict sprint performance in teenage sprinters. USJ120ms and UDJ120ms could better predict the acceleration (10m) and high-speed phase (50m) in sprint performance, respectively. Moreover, coaches and practitioners should be cautious when using only jump height or maximum force to predict sprint performance, since the results could be inaccurate when specific movement variables are not thoughtfully considered. Level of evidence III. Objectives Methods age (age 142 14 2 14± years 1682 168 168± cm weight 611 61 1 61± kg bilateralunilateral platform SJ, , (SJ) b USJ, USJ (USJ) c DJ, (DJ) d UDJ. UDJ . (UDJ) m day 10m times ms measures Results SJms USJms UDJms DJ120ms DJms observed Conclusion (10m highspeed high speed (50m respectively Moreover considered III 16 6 (SJ (USJ (DJ (UDJ

RESUMO Objetivos: Este estudo teve como objetivo examinar a relação entre o salto vertical e a força em tempo específico e o desempenho de sprint em velocistas adolescentes. Métodos: Quinze adolescentes velocistas do sexo masculino (idade: 14 ± 2 anos, estatura: 168 ± 2 cm, peso: 61 ± 1 kg) participaram do estudo. Os indivíduos realizaram os seguintes saltos bilaterais e unilaterais em uma plataforma de força: a) squat jump (SJ), b) SJ unilateral (USJ), c) drop jump (DJ) de 40 cm e d) DJ unilateral (UDJ) de 20cm. O teste de sprint de 60 m foi realizado no segundo dia. Os cronômetros rastreadores para treinos fracionados foram posicionados para registrar os tempos fracionados de 5 m, 10 m, 50 m e 60 m. As variáveis para inclusão foram altura do salto vertical, força máxima e saída de força a 120 m em todos os saltos e medidas de tempo do sprint. Resultados: Os resultados da análise da correlação produto-tempo de Pearson mostraram que o SJ de 120 m foi correlacionado com 5 m e USJ de 120 m foi correlacionado com 10 m. O UDJ de 120 m teve correlação mais forte com DJ de 50 m do que de 120 m. Embora tenham sido observadas correlações significativas com força e altura máximas, alguns resultados foram inconsistentes entre os saltos bilaterais e unilaterais. Conclusões: Nossos resultados destacaram que os saltos com forma semelhante a certas saídas de força no tempo específico do evento podem prever com mais precisão o desempenho no sprint em adolescentes velocistas. O USJ de 120 m e o UDJ de 120 m podem prever melhor, respectivamente, a aceleração (10 m) e a fase de alta velocidade (50 m) no desempenho no sprint. Além disso, treinadores e praticantes devem ser cautelosos ao usar apenas a altura do salto ou a força máxima para prever o desempenho no sprint, uma vez que os resultados podem ser imprecisos quando variáveis específicas do movimento não forem consideradas com precisão. Nível de evidência III. Objetivos Métodos idade (idade anos estatura 16 peso 6 kg SJ, , (SJ) b USJ, (USJ) c (DJ 4 d (UDJ 20cm dia 12 Resultados produtotempo produto máximas Conclusões melhor respectivamente (1 (5 disso III (SJ (USJ (

RESUMO Objetivos: Este estudo teve como objetivo construir um perfil de índices específicos de aptidão física para velocistas adolescentes do sexo masculino da Seleção Nacional da China, que proporcione uma base para avaliação da aptidão para corridas para treinamento de adolescentes. Materiais e Métodos: Foram recrutados 229 adolescentes do sexo masculino e velocistas de nível equivalente para participar deste teste de índices. Foram usados o teste t e o teste Kruskal-Wallis para a primeira seleção dos índices de aptidão física. Na segunda seleção foi aplicada a análise dos componentes principais para selecionar fatores comuns com maiores valores característicos. Os índices de aptidão física analisados foram estatura, comprimento das pernas, medida B (circunferência do tornozelo/comprimento do calcanhar x 100%) e medida A (circunferência da coxa/comprimento da perna x 100%), hemoglobina, tempo de sprint 60 metros, tempo de sprint de 100 metros, salto de contramovimento (CMJ), velocidade máxima de salto de contramovimento, tempo de voo do CMJ, força máxima do CMJ, força do CMJ. Resultados: Foram selecionados 13 índices para avaliar as aptidões específicas dos velocistas da amostra, divididos em 3 categorias principais e 9 subcategorias. O peso de cada índice de aptidão foi confirmado e usado para construir uma escala de avaliação de aptidão padrão. Conclusões: Os índices antropométricos indicam os limites inerentes aos atletas na estrutura do movimento do sprint. Os indicadores fisiológicos indicam o potencial do atleta de gastar energia e recuperá-la em curto período. Os índices motores indicam a capacidade máxima do atleta no sprint, o poder de reação dos membros inferiores, a força e a potência máxima. Nível de evidência II, Estudos diagnósticos - Investigação de um exame para diagnóstico.

ABSTRACT Objectives: This study aimed to construct a profile of specific fitness indices for male teenage sprinters on the Chinese National Team to provide sprinting fitness assessments for teenage training. Material and Methods: 229 male teenage sprinters at the same level were recruited to participate in this test for the indices. The t- and Kruskal-Wallis tests were conducted for the first selection of fitness indices. In the second selection, principal components analysis was applied to select common factors with greater characteristic values. The fitness indices chosen were height, leg length, measurement B (ankle circumference/heel length×100%) and measurement A (thigh circumference/leg length×100%), hemoglobin, 60m sprint time, 100m sprint time, countermovement jump (CMJ), maximum countermovement jump velocity, CMJ flight time, CMJ maximum force, and CMJ force. Results: Thirteen indices were chosen for the specific fitness of male teenage Chinese male sprinters with 3 general categories and 9 subcategories. The weight of each fitness index was confirmed and used to construct a standard fitness assessment scale. Conclusion: Anthropometric indices indicate the athlete’s innate limits in the structure of the sprinting motion. Physiological indices indicate the athlete’s potential to expend energy and recover in a short time. Motor indices indicate the athlete’s maximum sprinting ability, lower limb reaction strength, power, and maximum strength. Level of evidence II, Diagnostic studies - Investigation of a diagnostic test.

RESUMEN Objetivos: Este estudio tuvo por finalidad el desarrollo de un perfil de índices de aptitud física específico para velocistas masculinos adolescentes de la Selección Nacional de China, que proporcione una base para la evaluación de la aptitud física en velocidad durante el entrenamiento de adolescentes. Materiales y Métodos: En esta prueba de índices participaron 229 velocistas adolescentes masculinos de nivel equivalente. En la primera selección de índices de aptitud se utilizó la Prueba T y la Prueba Kruskal-Wallis. En la segunda selección se aplicó el análisis de componentes principales para identificar factores comunes con mayores valores característicos. Los índices de aptitud física analizados fueron: altura, longitud de las piernas, medida B (circunferencia del tobillo / longitud del talón x 100%), y medida A (circunferencia del muslo / longitud de la pierna x 100%) × 100%, hemoglobina, tiempo de sprint de 60 m, tiempo de sprint de 100 m, salto de contramovimiento (CMJ), velocidad máxima del salto de contramovimiento, tiempo de vuelo del CMJ, fuerza máxima del CMJ, fuerza del CMJ. Resultados: Se seleccionaron 13 índices para evaluar la aptitud física específica de los velocistas del estudio, divididos en 3 categorías principales y 9 subcategorías. Se confirmó la ponderación de cada índice de aptitud física y se utilizó para crear una escala estándar de evaluación de la aptitud. Conclusión: Los índices antropométricos reflejan los límites innatos de los atletas en la estructura del movimiento del sprint. Los índices fisiológicos indican el potencial del atleta para gastar energía y recuperarla en un corto período. Los índices motores indican la capacidad máxima del atleta en el sprint, la potencia de reacción de las extremidades inferiores, la fuerza y la potencia máxima. Nivel de evidencia II, Estudios diagnósticos - Investigación de un examen para diagnóstico.

This study aimed to compare the ability of narrow-band imaging to detect early and invasive lung cancer with that of conventional pathological analysis and white-light bronchoscopy. We searched the PubMed, EMBASE, Sinomed, and China National Knowledge Infrastructure databases for relevant studies. Meta-disc software was used to perform data analysis, meta-regression analysis, sensitivity analysis, and heterogeneity testing, and STATA software was used to determine if publication bias was present, as well as to calculate the relative risks for the sensitivity and specificity of narrow-band imaging vs those of white-light bronchoscopy for the detection of early and invasive lung cancer. A random-effects model was used to assess the diagnostic efficacy of the above modalities in cases in which a high degree of between-study heterogeneity was noted with respect to their diagnostic efficacies. The database search identified six studies including 578 patients. The pooled sensitivity and specificity of narrow-band imaging were 86% (95% confidence interval: 83-88%) and 81% (95% confidence interval: 77-84%), respectively, and the pooled sensitivity and specificity of white-light bronchoscopy were 70% (95% confidence interval: 66-74%) and 66% (95% confidence interval: 62-70%), respectively. The pooled relative risks for the sensitivity and specificity of narrow-band imaging vs the sensitivity and specificity of white-light bronchoscopy for the detection of early and invasive lung cancer were 1.33 (95% confidence interval: 1.07-1.67) and 1.09 (95% confidence interval: 0.84-1.42), respectively, and sensitivity analysis showed that narrow-band imaging exhibited good diagnostic efficacy with respect to detecting early and invasive lung cancer and that the results of the study were stable. Narrow-band imaging was superior to white light bronchoscopy with respect to detecting early and invasive lung cancer; however, the specificities of the two modalities did not differ significantly.

BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1/2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1/2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex CONCLUSIONS: Our study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1/2 and reduced activity of its downstream target-cyclin D1.