Abstract For the design of scaled model and similarity prediction of vibration of 300,000m3 blast furnace gasholder body, based on energy method and discrete stiffening theory, the scaling principle relation of gasholder body free vibration are derived. By using the thickness scale factor of the gasholder body skin and rib is distorted, and utilizing the stiffness equivalence, respectively, gasholder body scaled models (stiffened rib equivalent to rectangle) with geometric distortion and with both geometric and material distortion are designed and fabricated respectively. The free vibration of distortion similarity prediction of gasholder body prototype is carried out by the method of simulation and test. Results show that both two kinds of gasholder body scaled models, can predict the prototype frequency more accurately by using the similarity coefficient calculated by the average bending stiffness of column, ring rib, and wind-resistant ring. The proposed design method and scaling principle relation for gasholder body scaled model with free vibration and distortion are suitable for the design of scaled model distortion and similar prediction of free vibration of longitudinal ring-stiffened large thin-walled cylindrical shells with T-shaped and I-shaped stiffeners. 300000m3 m 300 000m3 300,000m theory derived distorted equivalence respectively stiffened rectangle test column windresistant wind resistant ringstiffened thinwalled thin walled Tshaped T shaped Ishaped I stiffeners 300000m 30 000m 3

ABSTRACT: Owing to rich in vitamins, proteins, mineral elements, and active substances, the highly medicinal and economic value of mung bean seeds have been proven to be beneficial to human. In order to fully identificate and innovative utilization of excellent germplasm resources for mung bean yield and quality breeding, the genetic diversity level of eight seed phenotypic traits of 302 mung bean germplasm resources were comprehensively evaluated by diversity analysis, correlation analysis, principal component analysis (PCA), and cluster analysis. The results showed the genetic diversity of eight seed phenotypic traits of 302 mung bean germplasm resources with a Shannon diversity Index ranging from 0.25 to 2.09. For most traits, their coefficient of variation (CV) were more than 10% with the highest being seed shape (41.51%). Correlation analysis showed that the 100-seed weight had extremely positive correlations with seed size, seed length, seed width, and seed thickness. PCA of seed phenotypic traits showed that the cumulative contribution rate of the first three principal components was 78.551%. These principal components included seed width, seed coat gloss and seed color. The 302 mung bean germplasm resources were divided into eight groups by cluster analysis. A total of 140 germplasm resources with large-seed were selected from group I and group II, of which 9 germplasm resources in group II were likely to be high-yield varieties. Overall, this study not only indicated sufficient variation among the studied germplasm resources, but also provided a theoretical basis for the selection, utilization and hybrid breeding of mung bean germplasm resources. ABSTRACT vitamins proteins elements substances human 30 PCA, , (PCA) 025 0 25 0.2 209 2 09 2.09 CV (CV 10 41.51%. 4151 41.51% . 41 51 (41.51%) 100seed 100 size length width thickness 78551 78 551 78.551% color 14 largeseed large highyield high varieties Overall selection 3 (PCA 02 0. 20 2.0 1 415 41.51 4 5 (41.51% 7855 7 55 78.551 2. 41.5 (41.51 785 78.55 41. (41.5 78.5 (41. 78. (41 (4 (

RESUMO: Devido ao seu caráter rico em vitaminas, proteínas, elementos minerais e substâncias ativas, o valor altamente medicinal e econômico das sementes de feijão-mungo tem provado ser benéfico para o ser humano. A fim de identificar plenamente e utilizar de forma inovadora excelentes recursos de Germoplasma para a produção de feijão-mungo e reprodução de qualidade, o nível de diversidade genética do oito caracteres fenotípicos de sementes de 302 recursos de germoplasma de feijão-mungo foram avaliados de forma abrangente por meio de análise de diversidade, análise de correlação, análise de componentes principais (PCA) e análise de agrupamento. Os resultados mostraram a diversidade genética de oito características fenotípicas de sementes de 302 recursos de germoplasma de feijão-mungo com um Índice de diversidade Shannon variando de 0,25 a 2,09. A análise de correlação mostrou que o peso de 100 sementes apresentou correlações extremamente positivas com tamanho, comprimento, largura e espessura de sementes. A PCA das características fenotípicas das sementes mostrou que a taxa de contribuição cumulativa dos três primeiros componentes principais foi de 78,551%, sendo estes componentes principais a largura da semente, brilho do revestimento e cor da semente. Os 302 recursos de germoplasma de feijão-mungo foram divididos em oito grupos por análise de agrupamento. Foram selecionados 140 recursos de germoplasma com sementes grandes dos grupos I e II, dos quais nove recursos de germoplasma do grupo II provavelmente seriam variedades de alto rendimento. De forma geral, este estudo não só indicou variação suficiente entre os recursos de germoplasma estudados, como também forneceu uma base teórica para a seleção, utilização e melhoramento híbrido dos recursos de germoplasma de feijão-mungo. RESUMO vitaminas proteínas ativas feijãomungo feijão mungo humano qualidade 30 (PCA agrupamento 025 0 25 0,2 209 2 09 2,09 10 tamanho comprimento 78551 78 551 78,551% semente 14 rendimento geral estudados seleção feijãomungo. mungo. 3 02 0, 20 2,0 1 7855 7 55 78,551 2, 785 5 78,55 78,5 78,

Abstract NCED1 (9-cis-epoxy carotenoid dioxygenase) plays important roles in controlling ABA levels and drought stress tolerance. The relationships between sequence polymorphisms of the TaNCED1 gene and drought resistance in wheat were analysed. Four allelic variations of the TaNCED1-5B generated to drought resistance were identified. Four groups of KASP (kompetitive allele-specific PCR) markers were developed based on the four alleles and were verified in a natural population of 311 wheat cultivars. The distribution of the four alleles of TaNCED1-5B in the natural population was clarified. Among the allelic variations, 84.62% of the wheat varieties with TT/-/C/-,TT/C/C/-,TT/-/C/G or TT/C/C/G haplotypes were dryland cultivars. The common feature of the above four haplotypes is that they all contain the TT (98) and C (343) alleles at the TaNCED1-5B-TT/CG (98) and TaNCED1-5B-C/T (343) loci, which indicated that these two locis had highly positive correlation with drought resistance in wheat. NCED 9cisepoxy cisepoxy 9 cis epoxy dioxygenase tolerance TaNCED analysed TaNCED15B TaNCEDB 5B B identified kompetitive allelespecific allele specific PCR 31 cultivars clarified 8462 84 62 84.62 TT//C/,TT/C/C/,TT//C/G TTCTTCCTTCG TT/ /C/ ,TT/C/C/ ,TT/ /C/G G TTCCG 98 (98 343 (343 TaNCED15BTT/CG TaNCED15BTTCG TaNCEDBTTCG TT/CG CG TaNCED15BC/T TaNCED15BCT TaNCEDBCT C/T T loci 3 846 8 6 84.6 /C TTCC ,TT/C/C ,TT (9 34 (34 TaNCED15BTT BTT BTTCG TTCG TaNCED15BC BC BCT CT 84. ( (3 TaNCEDBTT TaNCEDBC

Resumo Fundamento A fibrilação atrial (FA) é uma complicação prevalente associada à levosimendana; no entanto, permanece incerto se existem disparidades nos efeitos da levosimendana na FA não pós-operatória e pós-operatória. Objetivos Este estudo teve como objetivo avaliar o efeito da levosimendana na FA não pós-operatória e pós-operatória conduzindo uma metanálise de ensaios clínicos randomizados (ECR). Métodos PubMed, Embase, Biblioteca Cochrane e outras bases de dados foram pesquisadas. Pares de revisores identificaram ECRs que compararam levosimendana e placebo ou outras terapias, e os resultados relataram dados de eventos de FA. Foram utilizados modelos de efeitos aleatórios (com nível de significância de 5%). Resultados Foram incluídos 29 ensaios elegíveis compreendendo 6.550 participantes, onze dos quais avaliaram a incidência de FA não pós-operatória e 18 incluíram FA pós-operatória. A análise revelou que a levosimendana elevou significativamente o risco de FA no grupo não pós-operatório (OR, 1,62; IC 95%: 1,19-2,20; p=0,002) e reduziu a incidência de FA no grupo pós-operatório (OR, 0,65; IC 95%: 0,44-0,96; p=0,03). A ocorrência de FA diminuiu mais significativamente em pacientes que usaram levosimendana após cirurgia cardíaca (OR, 0,53; IC 95%: 0,32-0,88; p=0,02) do que em pacientes que usaram levosimendana antes da cirurgia cardíaca (OR, 0,67; IC 95%: 0,42-1,06; p=0,09). O risco de FA foi significativamente elevado pela grande dose em bolus de levosimendana (dose em bolus ≥12 μg/kg) (OR, 1,44; IC 95%: 1,10-1,88; p=0,004) e diminuído pela pequena dose em bolus de levosimendana (dose em bolus <12 μg/kg) (OR, 0,64; IC 95%: 0,34-1,20; p=0,16). Conclusão A levosimendana foi associada a um aumento da incidência de FA não pós-operatória. O emprego da levosimendana foi eficaz na prevenção da FA pós-operatória. (FA entanto pósoperatória pós operatória pósoperatória. operatória. ECR. ECR . (ECR) PubMed Embase pesquisadas terapias com 5%. 5 5% 5%) 2 6550 6 550 6.55 participantes 1 pósoperatório operatório OR, OR (OR 1,62 162 62 95% 95 1,192,20 119220 1,19 2,20 19 20 1,19-2,20 p=0,002 p0002 p 0 002 0,65 065 65 0,440,96 044096 0,44 0,96 44 96 0,44-0,96 p=0,03. p003 p=0,03 03 p=0,03) 0,53 053 53 0,320,88 032088 0,32 0,88 32 88 0,32-0,88 p=0,02 p002 02 0,67 067 67 0,421,06 042106 0,42 1,06 42 06 0,42-1,06 p=0,09. p009 p=0,09 09 p=0,09) 12 ≥1 μg/kg μgkg μg kg 1,44 144 1,101,88 110188 1,10 1,88 10 1,10-1,88 p=0,004 p0004 004 <1 0,64 064 64 0,341,20 034120 0,34 1,20 34 0,34-1,20 p=0,16. p016 p=0,16 16 p=0,16) (ECR 655 55 6.5 1,6 9 192 1,192,2 11922 119 1,1 220 2,2 1,19-2,2 p=0,00 p000 00 0,6 440 0,440,9 04409 044 0,4 096 0,9 4 0,44-0,9 p00 p=0,0 0,5 05 320 0,320,8 03208 032 0,3 088 0,8 3 8 0,32-0,8 421 0,421,0 04210 042 106 1,0 0,42-1,0 ≥ 1,4 14 101 1,101,8 11018 110 188 1,8 1,10-1,8 < 341 0,341,2 03412 034 120 1,2 0,34-1,2 p01 p=0,1 6. 1, 1,192, 1192 11 22 2, 1,19-2, 0, 0,440, 0440 04 0,44-0, p0 p=0, 0,320, 0320 08 0,32-0, 0,421, 0421 0,42-1, 1,101, 1101 1,10-1, 0,341, 0341 0,34-1, 1,192 1,19-2 0,440 0,44-0 p=0 0,320 0,32-0 0,421 0,42-1 1,101 1,10-1 0,341 0,34-1 1,19- 0,44- p= 0,32- 0,42- 1,10- 0,34-

Abstract Background Atrial fibrillation (AF) is a prevalent complication associated with levosimendan; however, it remains uncertain whether there are any disparities in the effects of levosimendan on non-postoperative and postoperative AF. Objectives This study aimed to evaluate the levosimendan effect on non-postoperative and postoperative AF by conducting a meta-analysis of randomized control trials (RCTs). Methods PubMed, Embase, Cochrane Library, and other databases were searched. Pairs of reviewers identified RCTs that compared levosimendan and placebo or other therapies, and the results reported AF events data. Random effects models were used (at a significance level of 5%). Results Twenty-nine eligible trials comprising 6550 participants were included, eleven of which evaluated the non-postoperative AF incidence, and 18 included postoperative AF. The analysis revealed that levosimendan elevated the AF risk significantly in the non-postoperative group (OR, 1.62; 95% CI: 1.19-2.20; p=0.002) and reduced the AF incidence in the postoperative group (OR, 0.65; 95% CI: 0.44-0.96; p=0.03). AF occurrence decreased more significantly in patients who used levosimendan after cardiac surgery (OR, 0.53; 95% CI: 0.32-0.88; p=0.02) than in patients who used levosimendan before cardiac surgery (OR, 0.67; 95% CI: 0.42-1.06; p=0.09). Moreover, The AF risk was significantly elevated by levosimendan large bolus dose (bolus dose≥12 μg/kg) (OR, 1.44; 95% CI: 1.10-1.88; p=0.004) and decreased by small bolus dose of levosimendan (bolus dose<12 μg/kg) (OR, 0.64; 95% CI: 0.34-1.20; p=0.16). Conclusion Levosimendan was linked to an increased non-postoperative AF incidence. The employment of levosimendan was effective in preventing postoperative AF. (AF however nonpostoperative non metaanalysis meta RCTs. . (RCTs) PubMed Embase Library searched therapies data at 5%. 5 5% 5%) Twentynine Twenty nine 655 1 OR, OR (OR 1.62 162 62 95 CI 1.192.20 119220 1.19 2.20 19 2 20 1.19-2.20 p=0.002 p0002 p 0 002 0.65 065 65 0.440.96 044096 0.44 0.96 44 96 0.44-0.96 p=0.03. p003 p=0.03 03 p=0.03) 0.53 053 53 0.320.88 032088 0.32 0.88 32 88 0.32-0.88 p=0.02 p002 02 0.67 067 67 0.421.06 042106 0.42 1.06 42 06 0.42-1.06 p=0.09. p009 p=0.09 09 p=0.09) Moreover dose12 12 dose≥1 μg/kg μgkg μg kg 1.44 144 1.101.88 110188 1.10 1.88 10 1.10-1.88 p=0.004 p0004 004 dose<1 0.64 064 64 0.341.20 034120 0.34 1.20 34 0.34-1.20 p=0.16. p016 p=0.16 16 p=0.16) (RCTs 1.6 6 9 192 1.192.2 11922 119 1.1 220 2.2 1.19-2.2 p=0.00 p000 00 0.6 440 0.440.9 04409 044 0.4 096 0.9 4 0.44-0.9 p00 p=0.0 0.5 05 320 0.320.8 03208 032 0.3 088 0.8 3 8 0.32-0.8 421 0.421.0 04210 042 106 1.0 0.42-1.0 dose1 dose≥ 1.4 14 101 1.101.8 11018 110 188 1.8 1.10-1.8 dose< 341 0.341.2 03412 034 120 1.2 0.34-1.2 p01 p=0.1 1. 1.192. 1192 11 22 2. 1.19-2. 0. 0.440. 0440 04 0.44-0. p0 p=0. 0.320. 0320 08 0.32-0. 0.421. 0421 0.42-1. 1.101. 1101 1.10-1. 0.341. 0341 0.34-1. 1.192 1.19-2 0.440 0.44-0 p=0 0.320 0.32-0 0.421 0.42-1 1.101 1.10-1 0.341 0.34-1 1.19- 0.44- p= 0.32- 0.42- 1.10- 0.34-

ABSTRACT Introduction Main renal artery clamping and selective arterial clamping are two conventional devascularization methods for robot-assisted partial nephrectomy (RAPN) (1, 2). Decreasing warm ischemic (WI) time (3, 4) and improving clear surgical visualization (5) are the main surgically modifiable factors for RAPN, especially in large complex renal cancer (6). In this study, we described our surgical technique, focusing on gradual segmental artery unclamping on patients with large renal tumors. Material and methods Two patients (R.E.N.A.L score 10 and 11) underwent RAPN with gradual segmental artery unclamping (Figures 1 and 2). The unclamping included five key steps. First, all renal segmental arteries were identified as tumor feeding vessel(s) and the vessels for normal kidney parenchyma under the guidance of CT angiography (CTA) 3-division (3D) reconstruction. Second, all segmental arteries were isolated, and the feeding one(s) should be blocked before other arteries were blocked. Third, the tumor was resected outside the pseudocapsule, and the deep resection bed was sutured for initial hemostasis. Fourth, the segmental arteries were reopened except for the tumor feeding one(s), and normal kidney parenchyma restored blood supply. And fifth, the resection bed was completely sutured, and the feeding vessel supplying the tumor was opened after the suture. Warm ischemia time (WIT) was defined as the time measured between clamping and unclamping of the renal artery. WIT1 was the time for normal kidney parenchyma and WIT2 was the time for resection area. Patient demographics, perioperative variables, and warm ischemic time were included in our study. And we presented the details of gradual segmental artery unclamping in the video. Results In both cases, the total operation times were 215 and 130 mins for patient 1 and patient 2, respectively. WIT1 and WIT2 for patient 1 were 15 min and 33 min., and WIT1 and WIT2 for patient 2 were 21 min and 32 min, respectivelly. The maximum diameters of the masses resected were 10.8 and 7.3 cm, and surgical margins were negative. No patient had complications after operation. Preoperative and postoperative eGFR did not change significantly. Pre- and postoperative eGFR were 111 and 108 mL/min for patient 1, 91 and 83 mL/min for patient 2, respectively. Key hints for outcomes optimization during RAPN on patients with large complex renal tumors: 1) Each segmental renal artery is precised clamped before we excise the tumor, and an excellent surgical vision is essential for precising excision and shortening clamping time, 2) Other segmental renal arteries are unclamped except tumor feeding branch after suturing deep layer of parenchyma, and most normal parenchyma restores blood supply, 3) Preoperative high-resolution computed tomography angiography (CTA) and 3D reconstructive renal structure serve as a guide to clear the approach to find the tumor and segmental arteries (7, 8). Conclusions Gradual segmental artery unclamping is feasible and efficient to excise large complex renal cancer. Compared with main renal artery clamping, it can shorten the warm ischemic time of normal parenchyma; On the other hand, compared with selective segmental arterial clamping, the technique can reduce bleeding from the deep resection bed, keep a clear surgical vision, and decrease the incidence of positive margin. robotassisted robot assisted (RAPN (1 2. . WI (WI 3, 3 (3 4 5 (5 6. 6 (6) study tumors R.E.N.A.L RENAL R E N A L 11 Figures steps First s vessel(s CTA (CTA 3division division D (3D reconstruction Second isolated ones one one(s Third pseudocapsule hemostasis Fourth ones, , supply fifth suture WIT (WIT area demographics variables video cases 13 respectively min. respectivelly 8 10. 73 7 7. cm negative significantly Pre mLmin mL 9 highresolution high resolution 7, (7 8. 8) hand margin ( (6

Abstract Objective: To investigate the safety and efficacy of short-term (7-day) Dual Antiplatelet Therapy (DAPT) with intensive rosuvastatin in Acute Ischemic Stroke (AIS). Methods: In this study, patients with AIS in the emergency department of the hospital from October 2016 to December 2019 were registered and divided into the control group (Single Antiplatelet Therapy [SAPT] + rosuvastatin) and the study group (7-day DAPT + intensive rosuvastatin) according to the therapy regimens. The generalized linear model was used to compare the National Institute of Health Stroke Scale (NIHSS) scores between the two groups during the 21-day treatment. A Cox regression model was used to compare recurrent ischemic stroke, bleeding events, Statin-Induced Liver Injury (SILI), and Statin-Associated Myopathy (SAM) between the two groups during the 90-day follow-up. Results: Comparison of NIHSS scores after 21-day treatment: NIHSS scores in the study group decreased significantly, 0.273-times as much as that in the control group (Odds Ratio [OR] 0.273; 95% Confidence Interval [95% CI] 0.208–0.359; p < 0.001). Comparison of recurrent ischemic stroke during the 90-day follow-up: The therapy of the study group reduced the risk of recurrent stroke by 65% (7.76% vs. 22.82%, Hazard Ratio [HR] 0.350; 95% CI 0.167–0.730; p = 0.005). Comparison of bleeding events: There was no statistical difference between the two groups (7.79% vs. 6.71%, HR = 1.076; 95% CI 0.424–2.732; p = 0.878). No cases of SILI and SAM were found. Conclusions: Short-term DAPT with intensive rosuvastatin effectively relieved the clinical symptoms and significantly reduced the recurrent stroke for patients with mild-to-moderate AIS within 90 days, without increasing bleeding events, SILI and SAM. Objective shortterm short term 7day day 7 (DAPT AIS. . (AIS) Methods 201 Single SAPT [SAPT regimens (NIHSS 21day 21 treatment events StatinInduced Statin Induced SILI, , (SILI) StatinAssociated Associated (SAM 90day followup. followup follow up. up follow-up Results 0.273times 0273times times 0.273 0 273 Odds OR [OR 0273 95 [95 0.208–0.359 02080359 208 359 0.001. 0001 0.001 001 0.001) 65 7.76% 776 76 (7.76 vs 2282 22 82 22.82% [HR 0.350 0350 350 0.167–0.730 01670730 167 730 0.005. 0005 0.005 005 0.005) 7.79% 779 79 (7.79 671 6 71 6.71% 1.076 1076 1 076 0.424–2.732 04242732 424 2 732 0.878. 0878 0.878 878 0.878) found Conclusions Shortterm Short mildtomoderate mild moderate 9 days (AIS 20 (SILI 273times 0.27 27 027 [9 0.208–0.35 0208035 35 000 0.00 00 7.76 77 (7.7 228 8 22.82 0.35 035 0.167–0.73 0167073 16 73 7.79 67 6.71 1.07 107 07 0.424–2.73 0424273 42 087 0.87 87 0.2 02 [ 0.208–0.3 020803 3 0.0 7.7 (7. 22.8 0.3 03 0.167–0.7 016707 6.7 1.0 10 0.424–2.7 042427 4 08 0.8 0. 0.208–0. 02080 7. (7 22. 0.167–0. 01670 6. 1. 0.424–2. 04242 0.208–0 0208 ( 0.167–0 0167 0.424–2 0424 0.208– 020 0.167– 016 0.424– 042 0.208 0.167 01 0.424 04 0.20 0.16 0.42 0.1 0.4

This study evaluated the effects of perioperative nutrition management by a multidisciplinary team on nutrition and postoperative complications of patients with esophageal cancer. A total of 239 patients with esophageal cancer who underwent esophagectomy and gastric conduit reconstruction for esophageal or esophagogastric junction cancer between February 2019 and February 2020 were included in the study. They were divided into the experimental group (120 patients) and the control group (119 patients) using the random number table method. Control group patients received routine diet management and experimental group patients received perioperative nutrition management by a multidisciplinary team. The differences of nutriture and postoperative complications between the two groups were compared. At 3 and 7 days after surgery, the experimental group patients had higher total protein and albumin levels (P<0.05), shorter postoperative anal exhaust time (P<0.05), lower incidence of postoperative gastrointestinal adverse reactions, pneumonia, anastomotic fistula, hypoproteinemia (P<0.05), and lower hospitalization costs (P<0.05) than the control group. Nutrition management by a multidisciplinary team effectively improved the nutriture of patients, promoted the rapid recovery of postoperative gastrointestinal function, reduced postoperative complications, and reduced hospitalization costs. 23 201 202 120 (12 119 (11 method compared surgery P<0.05, P005 P P<0.05 , 0 05 reactions pneumonia fistula (P<0.05 function 2 20 12 (1 11 P00 P<0.0 (P<0.0 1 ( P0 P<0. (P<0. P<0 (P<0 P< (P< (P

This work presented a new PEG modified calix[4]arene stationary phase (C4A-PEG-2PTSC) for gas chromatographic (GC) separations. The statically coated C4A-PEG-2PTSC column exhibited moderate polarity and column efficiency of 2224 plates m-1 determined by 1-octanol at 120 oC. The results showed that the C4A-PEG-2PTSC column exhibited the good separation performance for various types of analytes containing halogenated benzenes, benzaldehydes, phenols, alcohols, anilines isomers and showed the different retention behavior with the comparative columns. Importantly, it showed the better separation capacity of toluidine and xylidine isomers than commercial HP-35 column. Moreover, it was applied in practical sample analysis to detect the content of isomer impurities. This work exhibited the good application potential of C4A-PEG-2PTSC stationary phase in GC analyses. calix4arene calixarene calix 4 arene C4APEG2PTSC CAPEGPTSC C4A 2PTSC C A PTSC (C4A-PEG-2PTSC (GC separations 222 m1 m 1 m- 1octanol octanol 12 oC benzenes benzaldehydes phenols alcohols columns Importantly HP35 HP 35 HP-3 Moreover impurities analyses APEG CA 22 HP3 3 HP- 2

Abstract Background: The accuracy of diagnosis and the safety of treatment in colonoscopy depends largely on the quality of bowel cleansing. This study aimed to compare the efficacy and adverse reactions of Polyethylene Glycol (PEG) combined with lactulose with that of PEG alone in bowel preparation before colonoscopy. Methods: The authors searched a number of databases including EMBASE, MEDLINE, Cochrane Library, and China Academic Journals Full-text Database. The authors screened according to literature inclusion and exclusion criteria, assessed the quality of the included literature, and extracted the data. The meta-analysis of included literature used RevMan 5.3 and Stata 14.0 software. Results: A total of 18 studies, including 2274 patients, were enrolled. The meta-analysis showed that PEG combined with lactulose had a better efficacy (OR = 3.87, 95% CI 3.07‒4.87, p = 0.000, and I2 = 36.2% in the efficiency group; WMD = 0.86, 95% CI 0.69‒1.03, p = 0.032 and I2 = 0% in the BBPS score group) in bowel preparation for patients with or without constipation. Moreover, PEG combined with lactulose had fewer adverse reactions, including abdominal pain (OR = 1.42, 95% CI 0.94‒2.14, p = 0.094), nausea (OR = 1.60, 95% CI 1.13‒2.28, p = 0.009) and vomiting (OR = 1.77, 95% CI 1.14‒2.74, p = 0.011), than PEG alone. No significant reduction in the incidence of abdominal distention was observed. Conclusion: PEG combined with lactulose may be a better choice for bowel preparation before colonoscopy compared with PEG alone. Background cleansing (PEG Methods EMBASE MEDLINE Library Fulltext Full text Database criteria data metaanalysis meta analysis 53 5 3 5. 140 14 0 14. software Results 1 studies 227 enrolled OR 387 87 3.87 95 307487 07 4 3.07‒4.87 0000 000 0.000 I 362 36 2 36.2 group 086 86 0.86 069103 69 03 0.69‒1.03 0032 032 0.03 constipation Moreover 142 42 1.42 094214 94 0.94‒2.14 0.094, 0094 0.094 , 094 0.094) 160 60 1.60 113228 13 28 1.13‒2.28 0.009 0009 009 177 77 1.77 114274 74 1.14‒2.74 0.011, 0011 0.011 011 0.011) observed Conclusion 22 38 8 3.8 9 30748 3.07‒4.8 00 0.00 36. 08 0.8 06910 6 0.69‒1.0 003 0.0 1.4 09421 0.94‒2.1 0.09 09 16 1.6 11322 1.13‒2.2 17 7 1.7 11427 1.14‒2.7 001 0.01 01 3. 3074 3.07‒4. 0. 0691 0.69‒1. 1. 0942 0.94‒2. 1132 1.13‒2. 1142 1.14‒2. 307 3.07‒4 069 0.69‒1 0.94‒2 113 1.13‒2 114 1.14‒2 30 3.07‒ 06 0.69‒ 0.94‒ 11 1.13‒ 1.14‒ 3.07 0.69 0.94 1.13 1.14 3.0 0.6 0.9 1.1

Acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) is a critical respiratory syndrome with limited effective interventions. Lung macrophages play a critical role in the pathogenesis of abnormal inflammatory response in the syndrome. Recently, impaired fatty acid oxidation (FAO), one of the key lipid metabolic signalings, was found to participate in the onset and development of various lung diseases, including ALI/ARDS. Lipid/fatty acid contents within mouse lungs were quantified using the Oil Red O staining. The protective effect of FAO activator L-carnitine (Lca, 50, 500, or 5 mg/mL) was evaluated by cell counting kit 8 (CCK-8) assay, real-time quantitative PCR (qPCR), ELISA, immunoblotting, fluorescence imaging, and fluorescence plate reader detection in lipopolysaccharide (LPS) (100 ng/mL)-stimulated THP-1-derived macrophages. The in vivo efficacy of Lca (300 mg/kg) was determined in a 10 mg/kg LPS-induced ALI mouse model. We found for the first time that lipid accumulation in pulmonary macrophages was significantly increased in a classical ALI murine model, which indicated disrupted FAO induced by LPS. Lca showed potent anti-inflammatory and antioxidative effects on THP-1 derived macrophages upon LPS stimulation. Mechanistically, Lca was able to maintain FAO, mitochondrial activity, and ameliorate mitochondrial dynamics. In the LPS-induced ALI mouse model, we further discovered that Lca inhibited neutrophilic inflammation and decreased diffuse damage, which might be due to the preservation of mitochondrial homeostasis. These results broadened our understanding of ALI/ARDS pathogenesis and provided a promising drug candidate for this syndrome. (ALI ARDS (ARDS interventions Recently , (FAO) signalings diseases ALIARDS Lipidfatty Lipid staining Lcarnitine L carnitine Lca, (Lca 50 500 mg/mL mgmL mg mL CCK8 CCK (CCK-8 assay realtime real qPCR, qPCR (qPCR) ELISA immunoblotting imaging (LPS 100 (10 ng/mLstimulated ngmLstimulated ng/mL stimulated ng THP1derived THPderived THP 1 300 (30 mgkg kg LPSinduced model antiinflammatory anti THP1 THP- stimulation Mechanistically activity dynamics damage homeostasis (FAO (CCK- (qPCR (1 mLstimulated ngmL 30 (3 (CCK ( 3

Abstract Background Fever is a common symptom of Idiopathic inflammatory myopathies (IIM). However, the exact correlation between fever and the prognosis of IIM is still unclear. This study aims to clarify if the IIM patients initiated with fever are associated with poorer outcomes. Methods This was a single-center retrospective cohort study. Data were collected from 79 newly diagnosed, treatment-naive IIM patients in the Affiliated Wuxi People's Hospital of Nanjing Medical University (Wuxi, Jiangsu, China) from November 2016 to June 2020. According to the presence or absence of fever at the onset, the IIM patients were divided into two groups(fever group n = 28, without fever group n = 51) Clinical characteristics, laboratory data, treatment, and outcomes were recorded. The Kaplan-Meier and log-rank tests were used to compare the all-cause mortality, relapse rate, and acute exacerbation of interstitial lung disease (AE-ILD) incidence. The association of fever with the outcomes was assessed in the unadjusted and adjusted forward logistic regression model. Results Compared with the non-fever group, the age at onset of the fever group was higher, and mechanic's hands (MH) and interstitial lung disease (ILD) were more common. Systemic inflammation (CRP and ESR) was significantly higher in the fever group, while the level of albumin(ALB) and muscle enzymes were lower. The fever group seemed to be received more aggressive treatment, with higher dose glucocorticoids and higher rates of intravenous immunoglobulins(IVIG) use. The all-cause mortality rate and the incidence rate of AE-ILD were higher in the fever group. Even adjusted for the age at onset and treatments, fever was significantly associated with AE-ILD and all-cause mortality. Conclusion Our study has demonstrated that fever at initial diagnosis is associated with AE-ILD and mortality. Fever should serve as an early clinical warning sign for poor outcomes in IIM patients. IIM. . (IIM) However unclear singlecenter single center 7 diagnosed treatmentnaive treatment naive Peoples People s Wuxi, (Wuxi Jiangsu China 201 2020 groupsfever groups 28 51 characteristics data recorded KaplanMeier Kaplan Meier logrank log rank allcause all cause AEILD AE ILD (AE-ILD model nonfever non mechanics mechanic MH (MH (ILD CRP ESR albuminALB albumin ALB albumin(ALB lower immunoglobulinsIVIG immunoglobulins IVIG immunoglobulins(IVIG use treatments (IIM 20 202 2 5

Abstract Background Dexmedetomidine (DEX), a specific α2-adrenergic receptor agonist, is protective against myocardial ischemia/reperfusion injury (MIRI). However, the association between DEX preconditioning-induced cardioprotection and mitophagy suppression remains unclear. Objective Hence, we aimed to investigate whether DEX preconditioning alleviates MIRI by suppressing mitophagy via α2-adrenergic receptor activation. Method Sixty isolated rat hearts were treated with or without DEX before inducing ischemia and reperfusion; an α2-adrenergic receptor antagonist, yohimbine (YOH), was also administered before ischemia, alone or with DEX. The heart rate (HR), left ventricular diastolic pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), maximal and minimal rate of left ventricular pressure development (±dp/dtmax), and myocardial infarction size were measured. The mitochondrial ultrastructure and autophagosomes were assessed using transmission electron microscopy. Mitochondrial membrane potential and reactive oxygen species (ROS) levels were measured using JC-1 and dichloride hydrofluorescein diacetate assays, respectively. The expression levels of the mitophagy-associated proteins Beclin1, LC3II/I ratio, p62, PINK1, and Parkin were detected by western blotting. Results Compared with the control group, in the ischemia/reperfusion group, the HR, LVDP, and ±dp/dtmax were remarkably decreased (p< 0.05), whereas LVEDP and infarct sizes were significantly increased (p< 0.05). DEX preconditioning significantly improved cardiac dysfunction reduced myocardial infarction size, maintained mitochondrial structural integrity, increased mitochondrial membrane potential, inhibited autophagosomes formation, and decreased ROS production and Beclin1, LC3II/I ratio, PINK1, Parkin, and p62 expression(p< 0.05). When DEX and YOH were combined, YOH canceled the effect of DEX, whereas the use of YOH alone had no effect. Conclusion Therefore, DEX preconditioning was cardioprotective against MIRI in rats by suppressing mitophagy via α2-adrenergic receptor activation. , (DEX) α2adrenergic αadrenergic α2 adrenergic α agonist ischemiareperfusion reperfusion MIRI. . (MIRI) However preconditioninginduced induced unclear Hence activation antagonist YOH, (YOH) HR (HR) LVDP (LVDP) enddiastolic end LVEDP, (LVEDP) ±dp/dtmax, dpdtmax dp dtmax (±dp/dtmax) microscopy (ROS JC1 JC 1 JC- assays respectively mitophagyassociated associated Beclin1 Beclin LC3III LCIII LC3II I LC II ratio p PINK1 PINK blotting group p< (p 0.05, 005 0.05 0 05 0.05) 0.05. integrity formation p6 expressionp< expressionp expression(p combined Therefore (DEX (MIRI (YOH (HR (LVDP (LVEDP (±dp/dtmax III LCII 00 0.0 0.

Resumo Fundamento A dexmedetomidina (DEX), um agonista específico do receptor α2-adrenérgico, é protetora contra lesão de isquemia/reperfusão miocárdica (I/R). No entanto, a associação entre a cardioproteção induzida pelo pré-condicionamento DEX e a supressão da mitofagia permanece pouco clara. Objetivo Portanto, nosso objetivo foi investigar se o pré-condicionamento com DEX alivia a I/R, suprimindo a mitofagia via ativação do receptor α2-adrenérgico. Método Sessenta corações de ratos isolados foram tratados com ou sem DEX antes de induzir isquemia e reperfusão; um antagonista do receptor α2-adrenérgico, a ioimbina (YOH), também foi administrado antes da isquemia, isoladamente ou com DEX. A frequência cardíaca (FC), pressão diastólica do ventrículo esquerdo (PDVE), pressão diastólica final do ventrículo esquerdo (PDFVE), taxa máxima e mínima de desenvolvimento da pressão ventricular esquerda (±dp/dtmax) e tamanho do infarto do miocárdio foram medidos. A ultraestrutura mitocondrial e as autofagossomas foram avaliadas por microscopia eletrônica de transmissão. O potencial de membrana mitocondrial e os níveis de espécies reativas de oxigênio (ROS) foram medidos usando os ensaios JC-1 e diacetato de diclorodi hidrofluoresceína, respectivamente. Os níveis de expressão das proteínas associadas à mitofagia Beclin1, relação LC3II/I, p62, PINK1 e Parkin foram detectados por western blotting. Resultados Em comparação com o grupo controle, no grupo isquemia/reperfusão, a FC, PDVE e ±dp/dtmax foram notavelmente diminuídas (p<0,05), enquanto os tamanhos da PDFVE e do infarto aumentaram significativamente (p<0,05). O pré-condicionamento com DEX melhorou significativamente a disfunção cardíaca, reduziu o tamanho do infarto do miocárdio, manteve a integridade estrutural mitocondrial, aumentou o potencial de membrana mitocondrial, inibiu a formação de autofagossomas e diminuiu a produção de ROS e a relação Beclin1, relação LC3II/I, expressão PINK1, Parkin e p62(p<0,05). Quando DEX e YOH foram combinados, o YOH cancelou o efeito da DEX, enquanto o uso de YOH sozinha não teve efeito. Conclusão Portanto, o pré-condicionamento DEX foi cardioprotetor contra I/R em ratos, suprimindo a mitofagia por meio da ativação do receptor α2-adrenérgico. , (DEX) α2adrenérgico, α2adrenérgico αadrenérgico α2 adrenérgico, adrenérgico α α2-adrenérgico isquemiareperfusão reperfusão I/R. IR . I R (I/R) entanto précondicionamento pré condicionamento clara Portanto α2adrenérgico. adrenérgico. YOH, (YOH) FC (FC) PDVE, (PDVE) PDFVE, (PDFVE) dpdtmax dp dtmax (±dp/dtmax transmissão (ROS JC1 JC 1 JC- hidrofluoresceína respectivamente Beclin1 Beclin LC3III LCIII LC3II LC II LC3II/I p62 p PINK blotting controle p<0,05, p005 p<0,05 0 05 (p<0,05) p<0,05. p62p<0,05. p62p005 pp p62(p<0,05) combinados (DEX (I/R (YOH (FC (PDVE (PDFVE III LCII p6 p00 p<0,0 (p<0,05 p62p p62p<0,05 p62p00 p62(p<0,05 p0 p<0, (p<0,0 p62p<0,0 p62p0 p62(p<0,0 p<0 (p<0, p62p<0, p62(p<0, p< (p<0 p62p<0 p62(p<0 (p< p62p< p62(p< (p p62(p

Abstract Compound Danshen Dripping Pills (CDDPs) have been used in clinical treatment to protect the heart from ischemia/reperfusion (IR) injury for many years. However, the underlying mechanism implicated in the protective effects remains to be explored. Here, we determined the effects of CDDPs in Sprague-Dawley rats with the IR model. Cardiac function in vivo was assessed by echocardiography. Transmission electron microscopy, histological and immunohistochemical techniques, Western blotting and recombinant adeno-associated virus 9 transfection were used to illustrate the effects of CDDPs on IR and autophagy. Our results showed that pretreatment with CDDPs decreased the level of serum myocardial enzymes and infarct size in rats after IR. Apoptosis evaluation showed that CDDPs significantly ameliorated the cardiac apoptosis level after IR. Meanwhile, CDDPs pretreatment increased myocardial autophagic flux, with upregulation of LC3B, downregulation of p62, and increased autophagosomes and autolysosomes. Moreover, the autophagic flux inhibitor chloroquine could increase IR injury, while CDDPs could partially reverse the effects. Furthermore, our results showed that the activation of AMPK/mTOR was involved in the cardioprotective effect exerted by CDDPs. Herein, we suggest that CDDPs partially protect the heart from IR injury by enhancing autophagic flux through the activation of AMPK/mTOR. (CDDPs ischemiareperfusion ischemia reperfusion (IR years However explored Here SpragueDawley Sprague Dawley model echocardiography microscopy techniques adenoassociated adeno associated autophagy Meanwhile LC3B LCB LC B p62 p autolysosomes Moreover Furthermore AMPKmTOR AMPK mTOR Herein p6

RESUMO Introdução A técnica no arremesso de peso e a habilidade na etapa de arremessar são dois fatores importantes que determinam a habilidade dos atletas Especialistas e treinadores qualificados atribuem grande importância ao treinamento e pesquisa sobre a habilidade da fase de arremesso e a capacidade de arremessar. Objetivo Comparar o treinamento com carga gravitacional e o treinamento com carga incremental única através de meios experimentais, analisando os impactos na habilidade de arremesso nos atletas. Métodos O método de auto-avaliação foi usado para realizar o experimento comparativo de diferentes formas de treinamento de força em 20 estudantes universitários. O esquema experimental adota o treinamento único de força de carga incremental, e o esquema de controle adota o treinamento tradicional de barra. Resultados : Após o experimento, o ângulo da mão do grupo I foi significativamente maior do que o do grupo II (P < 0,05); após o experimento, o ângulo do ombro do grupo I foi significativamente maior do que o do grupo II (P < 0,05); após o experimento, o desempenho do tiro sentado colocado no grupo I foi significativamente maior do que o do grupo II (P < 0,05), e não houve diferença significativa no desempenho em pé (P > 0,05). Conclusão O treinamento com carga incremental única pode melhorar significativamente a capacidade de arremesso dos atletas de arremesso de peso. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction The technique in the shot put and the ability in the throwing stage are two important factors that determine the ability of athletes. Qualified experts and coaches attach great importance to training and research on the ability of the throwing stage and the ability to throw. Objective Compare gravity load training and single incremental load training through practical means, analyzing the impacts on throwing ability in athletes. Methods The self-assessment method was used to conduct the comparative experiment on different forms of strength training in 20 college students. The experimental scheme adopts the single incremental load strength training, and the control scheme adopts the traditional barbell training. Results : After the experiment, the hand angle of group I was significantly higher than that of group II (P < 0.05); after the experiment, the shoulder angle of group I was significantly higher than that of group II (P < 0.05); after the experiment, the performance of the seated shot placed in group I was significantly higher than that of group II (P < 0.05), and there was no significant difference in the standing performance (P > 0.05). Conclusion Single incremental load training can significantly improve the throwing ability of shot put athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción La técnica en el lanzamiento de peso y la habilidad en la fase de lanzamiento son dos factores importantes que determinan la habilidad de los atletas. Los especialistas y entrenadores cualificados atribuyen gran importancia a la formación y a la investigación sobre la habilidad de la fase de lanzamiento y la habilidad de lanzamiento. Objetivo Comparar el entrenamiento con carga gravitacional y el entrenamiento con carga incremental única a través de medios experimentales, analizando los impactos en la capacidad de lanzamiento en los atletas. Métodos Se utilizó el método de autoevaluación para realizar el experimento comparativo de diferentes formas de entrenamiento de fuerza en 20 estudiantes universitarios. El esquema experimental adopta el entrenamiento de fuerza con una sola carga incremental, y el esquema de control adopta el entrenamiento tradicional con barra. Resultados : Después del experimento, el ángulo de la mano del grupo I fue significativamente mayor que el del grupo II (P < 0,05); después del experimento, el ángulo del hombro del grupo I fue significativamente mayor que el del grupo II (P < 0,05); después del experimento, el rendimiento del lanzamiento de peso sentado del grupo I fue significativamente mayor que el del grupo II (P < 0,05), y no hubo diferencias significativas en el rendimiento de pie (P > 0,05). Conclusión El entrenamiento con una sola carga incremental puede mejorar significativamente la capacidad de lanzamiento de los atletas de lanzamiento de peso. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract Aiming to provide a new way that preserves more active components in Eucommia ulmoides (EU) leaves, this paper utilizes 4 methods, i.e., microwave, steam, air heating, and frying, to process EU leaves before drying, and their respective effects on the leaves were evaluated. The antioxidant activity of EU leaves in vitro was measured by the DPPH free radical method; the contents of total flavonoids (TFC) and total phenols (TPC) were analyzed by spectrophotometry; and the contents of main active components were determined by high-performance liquid chromatography (HPLC). The results showed the pre-process improved the quality of the EU leaves. As a simple and fast process, the microwave process is the most effective technique in the retention of flavonoids and four active ingredients except aucubin. High power (800 W) and short time (2.5 min) are more conducive to the retention of active ingredients, with high antioxidant activity. Appropriate steam and frying processes also exhibited benefits for the active components. In particular, the samples treated with steam for 10 minutes showed great potential for industrial application with the highest antioxidant activity and polyphenol content. Therefore, proper pre-processing before drying should be conducted to effectively protect the active components of EU leaves.