RESUMO: A lesão hepática aguda (ALI) é um problema médico importante que requer terapia eficaz. A astaxantina (AST) é um carotenóide, e os efeitos benéficos da astaxantina, incluindo atividades antioxidantes, anti-inflamatórias e antitumorais, foram identificados. O presente estudo foi desenhado para elucidar os efeitos protetores da astaxantina contra ALI e seus mecanismos subjacentes. Macrófagos RAW264.7 foram tratados com dimetil sulfóxido combinado com diferentes doses de astaxantina por 12 h. Os camundongos foram alimentados com ou sem astaxantina por até sete dias. O LPS foi administrado para induzir a inflamação. Histopatologia, estresse oxidativo, inflamação e apoptose foram avaliados. Os resultados indicaram que a astaxantina atenuou o estresse oxidativo induzido por LPS, inflamação e apoptose celular in vivo e in vitro. Experimentos in vivo e in vitro mostraram que a astaxantina regulou negativamente as vias de sinalização do fator nuclear-kappa beta (NF-κB), fator nuclear 2 relacionado ao eritróide 2 (Nrf2) e domínio de pirina da família NLR contendo 3 (NLRP3), inibindo o LPS- resposta inflamatória induzida, estresse oxidativo e apoptose celular e alívio da ALI induzida por LPS em camundongos. RESUMO (ALI eficaz AST (AST carotenóide antioxidantes antiinflamatórias anti inflamatórias antitumorais identificados subjacentes RAW2647 RAW RAW264 7 RAW264. 1 h dias Histopatologia avaliados nuclearkappa kappa NFκB, NFκB NF κB , (NF-κB) Nrf2 Nrf (Nrf2 NLRP3, NLRP3 NLRP (NLRP3) RAW26 (NF-κB (Nrf (NLRP3 RAW2 (NLRP

ABSTRACT: Acute liver injury (ALI) is an important medical problem that requires effective therapy. Astaxanthin (AST) is a carotenoid, and the beneficial effects of astaxanthin, including anti-oxidative, anti-inflammatory and anti-tumour activities, have been identified. The present study was designed to elucidate the protective effects of astaxanthin against ALI and their underlying mechanisms. RAW264.7 macrophages were treated with dimethyl sulfoxide combined with different doses of astaxanthin for 12 h. Mice were fed with or without astaxanthin for up to 7 days. LPS was administered to induce inflammation. We assessed histopathology, oxidative stress, inflammation and apoptosis .The results indicated that astaxanthin attenuated LPS-induced oxidative stress, inflammation and cell apoptosis both in vivo and in vitro. In vivo and in vitro experiments showed that astaxanthin down regulated the nuclear factor-kappa beta (NF-κB), nuclear factor erythroid 2-related factor 2 (Nrf2) and NLR family pyrin domain containing 3 (NLRP3) signalling pathways, inhibiting the LPS-induced inflammatory response, oxidative stress and cell apoptosis, and alleviating LPS-induced ALI in mice. ABSTRACT (ALI therapy AST (AST carotenoid antioxidative, antioxidative anti oxidative, anti-oxidative antiinflammatory antitumour tumour activities identified mechanisms RAW2647 RAW RAW264 RAW264. 1 h days histopathology LPSinduced induced factorkappa kappa NFκB, NFκB NF κB , (NF-κB) 2related related Nrf2 Nrf (Nrf2 NLRP3 NLRP (NLRP3 pathways response mice RAW26 (NF-κB (Nrf (NLRP RAW2

Forkhead Box O1 (FOXO1) has been reported to play important roles in many tumors. However, FOXO1 has not been studied in pan-cancer. The purpose of this study was to reveal the roles of FOXO1 in pan-cancer (33 cancers in this study). Through multiple public platforms, a pan-cancer analysis of FOXO1 was conducted to obtained FOXO1 expression profiles in various tumors to explore the relationship between FOXO1 expression and prognosis of these tumors and to disclose the potential mechanism of FOXO1 in these tumors. FOXO1 was associated with the prognosis of multiple tumors, especially LGG (low grade glioma), OV (ovarian carcinoma), and KIRC (kidney renal clear cell carcinoma). FOXO1 might play the role of an oncogenic gene in LGG and OV, while playing the role of a cancer suppressor gene in KIRC. FOXO1 expression had a significant correlation with the infiltration of some immune cells in LGG, OV, and KIRC. By combining FOXO1 expression and immune cell infiltration, we found that FOXO1 might influence the overall survival of LGG through the infiltration of myeloid dendritic cells or CD4+ T cells. Functional enrichment analysis and gene set enrichment analysis showed that FOXO1 might play roles in tumors through immunoregulatory interactions between a lymphoid and a non-lymphoid cell, TGF-beta signaling pathway, and transcriptional misregulation in cancer. FOXO1 was associated with the prognosis of multiple tumors, especially LGG, OV, and KIRC. In these tumors, FOXO1 might play its role via the regulation of the immune microenvironment. O FOXO (FOXO1 However pancancer. pancancer pan 33 (3 study. . study) platforms low glioma, glioma , glioma) ovarian carcinoma, carcinoma carcinoma) kidney carcinoma. CD4 CD nonlymphoid non TGFbeta TGF beta pathway microenvironment (FOXO 3 (

ABSTRACT In this study, a flexible capacitive pressure sensor was developed, featuring a dielectric layer composed of a polydimethylsilane (PDMS)/BaTiO3/SrTiO3 composite material. The electrode and dielectric layers were structured with a dual microstructure, combining diagonal and sandpaper-porous elements. Additionally, nano-barium titanate (BT) powder, known for its relatively high dielectric constant, was incorporated into PDMS, along with an appropriate amount of strontium titanate (STO), to enhance the sensor’s sensitivity. The developed sensor exhibited a remarkable sensitivity of 2.681 kPa–1, with response and release times of approximately 39 ms and 61 ms, respectively. It demonstrated a low detection threshold and withstood over 5000 compression cycles, showcasing excellent repeatability. The results underscored the sensor’s robust pressure-sensing performance, making it suitable for diverse applications, including human pulse monitoring, heartbeat tracking, robot arm sensing, object weight detection, and real-time healthcare monitoring. study PDMS/BaTiO3/SrTiO3 PDMSBaTiO3SrTiO3 PDMSBaTiOSrTiO PDMS /BaTiO3/SrTiO3 BaTiO3 SrTiO3 BaTiO SrTiO (PDMS)/BaTiO3/SrTiO material microstructure sandpaperporous sandpaper porous elements Additionally nanobarium nano barium BT (BT powder constant STO, STO , (STO) sensors s 2681 2 681 2.68 kPa1 kPa 1 kPa–1 3 6 respectively 500 cycles repeatability pressuresensing sensing performance applications monitoring tracking realtime real time PDMS/BaTiO3/SrTiO PDMSBaTiO PDMSBaTiO3SrTiO BaTiO3SrTiO3 BaTiOSrTiO /BaTiO3/SrTiO (STO 268 68 2.6 kPa– 50 BaTiO3SrTiO 26 2. 5

Resumo Fundamento: O antibiótico quimioterápico antraciclina doxorrubicina (DOX) pode induzir cardiotoxicidade cumulativa e levar à disfunção cardíaca. RNAs não codificantes longos (lncRNAs) podem funcionar como importantes reguladores na lesão miocárdica induzida por DOX. Objetivo: Este estudo tem como objetivo investigar o papel funcional e o mecanismo molecular do RNA antisense lncRNA OXCT1 1 (OXCT1-AS1) na lesão celular miocárdica induzida por DOX in vitro. Métodos: Cardiomiócitos humanos (AC16) foram estimulados com DOX para induzir um modelo de lesão celular miocárdica. A expressão de OXCT1-AS1, miR-874-3p e BDH1 em células AC16 foi determinada por RT-qPCR. A viabilidade das células AC16 foi medida pelo ensaio XTT. A citometria de fluxo foi empregada para avaliar a apoptose de células AC16. Western blotting foi utilizado para avaliar os níveis proteicos de marcadores relacionados à apoptose. O ensaio repórter de luciferase dupla foi conduzido para verificar a capacidade de ligação entre miR-874-3p e OXCT1-AS1 e entre miR-874-3p e BDH1. O valor de p<0,05 indicou significância estatística. Resultados: A expressão de OXCT1-AS1 foi diminuída em células AC16 tratadas com DOX. A superexpressão de OXCT1-AS1 reverteu a redução da viabilidade celular e a promoção da apoptose celular causada pela DOX. OXCT1-AS1 está ligado competitivamente ao miR-874-3p para regular positivamente o BDH1. A superexpressão de BDH1 restaurou a viabilidade das células AC16 e suprimiu a apoptose celular sob estimulação com DOX. A derrubada do BDH1 reverteu a atenuação da apoptose de células AC16 mediada por OXCT1-AS1 sob tratamento com DOX. Conclusão: LncRNA OXCT1-AS1 protege células miocárdicas humanas AC16 da apoptose induzida por DOX através do eixo miR-874-3p/BDH1. Fundamento (DOX cardíaca lncRNAs (lncRNAs Objetivo OXCT OXCT1AS1 OXCTAS AS1 AS (OXCT1-AS1 vitro Métodos AC (AC16 OXCT1AS1, AS1, miR8743p miRp miR 874 3p p BDH AC1 RTqPCR. RTqPCR RT qPCR. qPCR RT-qPCR XTT OXCT1-AS p005 0 05 p<0,0 estatística Resultados Conclusão miR8743p/BDH1. miR8743pBDH1 miRpBDH 3p/BDH1. miR-874-3p/BDH1 OXCT1AS (OXCT1-AS (AC1 87 p00 p<0, miR8743p/BDH1 pBDH miR8743pBDH 3pBDH1 3p/BDH1 miR-874-3p/BDH (AC 8 p0 p<0 miR8743p/BDH 3pBDH 3p/BDH p<

Abstract Background: The anthracycline chemotherapeutic antibiotic doxorubicin (DOX) can induce cumulative cardiotoxicity and lead to cardiac dysfunction. Long non-coding RNAs (lncRNAs) can function as important regulators in DOX-induced myocardial injury. Objective: This study aims to investigate the functional role and molecular mechanism of lncRNA OXCT1 antisense RNA 1 (OXCT1-AS1) in DOX-induced myocardial cell injury in vitro. Methods: Human cardiomyocytes (AC16) were stimulated with DOX to induce a myocardial cell injury model. OXCT1-AS1, miR-874-3p, and BDH1 expression in AC16 cells were determined by RT-qPCR. AC16 cell viability was measured by XTT assay. Flow cytometry was employed to assess the apoptosis of AC16 cells. Western blotting was used to evaluate protein levels of apoptosis-related markers. Dual-luciferase reporter assay was conducted to verify the binding ability between miR-874-3p and OXCT1-AS1 and between miR-874-3p and BDH1. The value of p<0.05 indicated statistical significance. Results: OXCT1-AS1 expression was decreased in DOX-treated AC16 cells. Overexpression of OXCT1-AS1 reversed the reduction of cell viability and promotion of cell apoptosis caused by DOX. OXCT1-AS1 is competitively bound to miR-874-3p to upregulate BDH1. BDH1 overexpression restored AC16 cell viability and suppressed cell apoptosis under DOX stimulation. Knocking down BDH1 reversed OXCT1-AS1-mediated attenuation of AC16 cell apoptosis under DOX treatment. Conclusion: LncRNA OXCT1-AS1 protects human myocardial cells AC16 from DOX-induced apoptosis via the miR-874-3p/BDH1 axis. Background (DOX dysfunction noncoding non coding lncRNAs (lncRNAs DOXinduced induced Objective OXCT OXCT1AS1 OXCTAS AS1 AS (OXCT1-AS1 vitro Methods AC (AC16 model OXCT1AS1, AS1, miR8743p, miR8743p miRp miR 874 3p, 3p p BDH AC1 RTqPCR. RTqPCR RT qPCR. qPCR RT-qPCR apoptosisrelated related markers Dualluciferase Dual luciferase OXCT1-AS p005 0 05 p<0.0 significance Results DOXtreated treated stimulation OXCT1AS1mediated OXCTASmediated mediated treatment Conclusion miR8743p/BDH1 miR8743pBDH1 miRpBDH 3p/BDH1 miR-874-3p/BDH axis OXCT1AS (OXCT1-AS (AC1 87 p00 p<0. miR8743p/BDH pBDH miR8743pBDH 3pBDH1 3p/BDH (AC 8 p0 p<0 3pBDH p<

Abstract Fast calculations are widely required in the traditional applications and the emerging digital twin fields. For those considerations, a novel physics-based fast algorithm, namely generalized rotation-superposition method, is proposed for fast calculating linear elastic responses of generalized rotationally axisymmetric structures under arbitrary mechanical loads. This improved method breaks through the limitations of the previous basic rotation-superposition method in rotational similarity of load and structural axisymmetry, and greatly expands its application scope. In this paper, firstly, the basic theory of the rotation-superposition algorithm is introduced; secondly, the theoretical model of the generalized rotation-superposition method is established; thirdly, the effectiveness and accuracy are verified by using finite element simulations; finally, through a complex case study, the applicability of the generalized rotation-superposition method for complex engineering problems and its advantages in efficiently obtaining massive amounts of data are further illustrated. fields considerations physicsbased physics based rotationsuperposition rotation superposition loads axisymmetry scope paper firstly introduced secondly established thirdly simulations finally study illustrated

The overexpression of P-glycoprotein (P-gp/ABCB1) is a leading cause of multidrug resistance (MDR). Hence, it is crucial to discover effective pharmaceuticals that counteract ABCB1-mediated multidrug resistance. FRAX486 is a p21-activated kinase (PAK) inhibitor. The objective of this study was to investigate whether FRAX486 can reverse ABCB1-mediated multidrug resistance, while also exploring its mechanism of action. The CCK8 assay demonstrated that FRAX486 significantly reversed ABCB1-mediated multidrug resistance. Furthermore, western blotting and immunofluorescence experiments revealed that FRAX486 had no impact on expression level and intracellular localization of ABCB1. Notably, FRAX486 was found to enhance intracellular drug accumulation and reduce efflux, resulting in the reversal of multidrug resistance. Docking analysis also indicated a strong affinity between FRAX486 and ABCB1. This study highlights the ability of FRAX486 to reverse ABCB1-mediated multidrug resistance and provides valuable insights for its clinical application. Pglycoprotein P glycoprotein Pgp/ABCB1 PgpABCB1 PgpABCB gp/ABCB1 gp ABCB1 ABCB (P-gp/ABCB1 MDR. MDR . (MDR) Hence ABCB1mediated ABCBmediated mediated FRAX FRAX48 p21activated pactivated p21 activated p PAK (PAK inhibitor action CCK Furthermore Notably efflux application Pgp Pgp/ABCB gpABCB1 gpABCB gp/ABCB (P-gp/ABCB (MDR FRAX4 p2

Abstract Gastric cancer (GC) often develops resistance to cisplatin treatment, but while latent transforming growth factor β-binding protein (LTBP2) is recognized as a potential regulator in GC, its specific role in cisplatin resistance is not fully understood. This study investigated LTBP2’s impact on cisplatin resistance in GC. LTBP2 expression was assessed in various GC cell lines, and its correlation with cisplatin sensitivity was determined through cell viability assays. Lentivirus-mediated LTBP2 silencing in HGC-27 cells demonstrated enhanced cisplatin sensitivity, reduced cell proliferation, and inhibition of the NF-κB2/Bcl-3/cyclin D1 pathway. Additionally, transient transfection overexpressed the NFκB2 gene in LTBP2-silenced HGC-27/DDPR cells, restoring cisplatin sensitivity and upregulating p52/Bcl-3/cyclin D1. In conclusion, silencing LTBP2 could effectively inhibit cell proliferation and mitigate cisplatin resistance via the NFKB noncanonical pathway NFKB2 p52/Bcl-3/cyclin D1. These findings propose LTBP2 as a potential therapeutic target for overcoming cisplatin resistance in GC patients. (GC treatment βbinding β binding LTBP (LTBP2 understood LTBP2s LTBPs s lines assays Lentivirusmediated Lentivirus mediated HGC27 HGC 27 HGC-2 NFκB2/Bcl3/cyclin NFκB2Bcl3cyclin NFκBBclcyclin NF κB2/Bcl 3/cyclin κB2 Bcl 3 cyclin κB D Additionally NFκB LTBP2silenced LTBPsilenced silenced HGC27/DDPR HGC27DDPR HGCDDPR 27/DDPR DDPR p52/Bcl3/cyclin p52Bcl3cyclin pBclcyclin p52/Bcl p52 p conclusion patients (LTBP HGC2 2 HGC- Bcl3 κB2Bcl κBBcl 3cyclin 27DDPR p52Bcl pBcl p5

Jun N-terminal kinase pathway-associated phosphatase (JKAP) regulates CD4+ T-cell differentiation and immunity, which are linked to mental disorders. This study aimed to explore the relationships between JKAP and T helper 17 (Th17)/regulatory T (Treg) ratio, as well as their associations with anxiety and depression in postpartum women. Serum JKAP were measured by enzyme-linked immunosorbent assay and blood Th17 and Treg cells were measured by flow cytometry in 250 postpartum women. Anxiety and depression were evaluated by the 6-item State-Trait Anxiety Inventory (STAI6) and Edinburgh Postnatal Depression Scale (EPDS). Anxiety and depression rates were 22.0 and 28.4%, respectively, among postpartum women. Notably, JKAP was negatively associated with the STAI6 (P=0.002) and EPDS scores (P<0.001) in postpartum women and was lower in postpartum women with anxiety (P=0.023) or depression (P=0.002) than in those without. Moreover, JKAP was inversely related to Th17 cells and Th17/Treg ratio but positively correlated with Treg cells in postpartum women (all P<0.001). Interestingly, Th17 cells and Th17/Treg ratio were both positively associated with STAI6 and EPDS scores in postpartum women (all P<0.001). Furthermore, Th17 cells and Th17/Treg ratio were lower in postpartum women with anxiety or depression than in those without (all P<0.01). Nevertheless, Treg cells were not linked to anxiety or depression in postpartum women. JKAP was negatively associated with Th17 cells and Th17/Treg ratio; moreover, they all related to anxiety and depression in postpartum women, indicating that JKAP may be involved in postpartum anxiety and depression via interactions with Th17 cells. Nterminal N terminal pathwayassociated pathway (JKAP CD4 CD Tcell cell immunity disorders 1 Th17/regulatory Th17regulatory Thregulatory /regulatory regulatory Th (Treg enzymelinked enzyme Th1 25 6item item 6 StateTrait State Trait STAI (STAI6 EPDS. . (EPDS) 220 22 0 22. 284 28 4 28.4% respectively Notably P=0.002 P0002 P 002 (P=0.002 P<0.001 P0001 001 (P<0.001 P=0.023 P0023 023 (P=0.023 Moreover Th17Treg ThTreg P<0.001. P<0.001) Interestingly Furthermore P<0.01. P001 P<0.01 01 P<0.01) Nevertheless moreover 2 (STAI (EPDS 28.4 P=0.00 P000 00 (P=0.00 P<0.00 (P<0.00 P=0.02 P002 02 (P=0.02 P00 P<0.0 28. P=0.0 (P=0.0 (P<0.0 P0 P<0. P=0. (P=0. (P<0. P<0 P=0 (P=0 (P<0 P< P= (P= (P< (P

Resumo Fundamento A hipertrofia cardíaca patológica (HC) sustentada é um fator de risco independente para aumento da incidência e mortalidade de eventos cardiovasculares. Objetivos Esta pesquisa foi projetada para desvendar o papel do RNA não codificante longo (LncRNA) CCAT2 na progressão da HC. Métodos Procedimentos de constrição aórtica transversal (TAC) foram conduzidos para construir um modelo de HC in vivo induzido por sobrecarga de pressão. O tratamento com angiotensina II (Ang II) foi utilizado para induzir células hipertróficas de cardiomiócitos de rato H9c2. Resultados Os resultados in vivo mostraram que o silenciamento de CCAT2 reduziu a área de superfície dos cardiomiócitos, aliviou a fibrose cardíaca e diminuiu os níveis de β-MHC, ANP e BNP em modelos de camundongos HC. Os resultados in vitro revelaram que o knockdown de CCAT2 reduziu a área de superfície celular e atenuou os níveis de β-MHC, ANP e BNP em células hipertróficas H9c2. Além disso, o silenciamento de CCAT2 diminuiu os níveis de β-catenina ativa, GSK-3β fosforilada e genes alvo Wnt (c-Myc, ciclinaD1 e c-Jun) em camundongos HC e células H9c2 hipertróficas. É importante ressaltar que o tratamento com o ativador da via Wnt / β-catenina LiCl reverteu a supressão do knockdown de CCAT2 na área de superfície celular H9c2 e nos níveis de MHC, ANP e BNP. Conclusões Coletivamente, o silenciamento do CCAT2 desempenha um papel protetor contra a HC através da inativação da sinalização Wnt/β-catenina, o que sugere que o CCAT2 pode se tornar um alvo terapêutico promissor para o HC. (HC cardiovasculares LncRNA (LncRNA CCAT TAC (TAC pressão Ang Hc H c βMHC, βMHC β MHC β-MHC disso βcatenina catenina ativa GSK3β GSKβ GSK 3β cMyc, cMyc Myc, Myc (c-Myc ciclinaD cJun Jun c-Jun H9c Coletivamente Wnt/βcatenina, Wntβcatenina Wnt/β catenina, Wnt/β-catenina Wnt/βcatenina Wntβ

Abstract Background Sustained pathological cardiac hypertrophy (CH) is an independent risk factor for increased incidence and mortality of cardiovascular events. Objectives This research was designed to unravel the role of long non-coding RNA (LncRNA) CCAT2 in CH progression. Methods Transverse aortic constriction (TAC) procedures were conducted to construct a pressure overload-induced in vivo CH model. Angiotensin II (Ang II) treatment was utilized to induce hypertrophic rat cardiomyocyte H9c2 cells. Results In vivo results showed that silencing of CCAT2 reduced cardiomyocyte surface area, alleviated cardiac fibrosis, and decreased β-MHC, ANP, and BNP levels in CH mouse models. In vitro results revealed that CCAT2 knockdown reduced cell surface area and attenuated β-MHC, ANP, and BNP levels in hypertrophic H9c2 cells. Besides, CCAT2 silencing decreased the levels of active β-catenin, phosphorylated-GSK-3β, and Wnt target genes (c-Myc, cyclinD1, and c-Jun) in CH mice and hypertrophic H9c2 cells. Importantly, treatment with the Wnt/β-catenin pathway activator LiCl reversed the suppression of CCAT2 knockdown on H9c2 cell surface area and MHC, ANP, and BNP levels. Conclusions Collectively, CCAT2 silencing plays a protective role against CH through inactivating the Wnt/β-catenin signaling, which suggests that CCAT2 might become a promising therapeutic target for CH. (CH events noncoding non coding LncRNA (LncRNA CCAT progression TAC (TAC overloadinduced overload induced model Ang Hc H c H9c cells fibrosis βMHC, βMHC β MHC β-MHC ANP models Besides βcatenin, βcatenin catenin, catenin β-catenin phosphorylatedGSK3β, phosphorylatedGSK3β phosphorylatedGSKβ phosphorylated GSK 3β, 3β phosphorylated-GSK-3β cMyc, cMyc Myc, Myc (c-Myc cyclinD1 cyclinD cJun Jun c-Jun Importantly Wnt/βcatenin Wntβcatenin Wnt/β Collectively signaling phosphorylatedGSK Wntβ

Abstract Backgroud Homocitrulline (Hcit), is involved in the pathological processes of some diseases. However, the role and function of Hcit (CBL) in human skin remains largely obscure. Objective To investigate the correlation of the level of Hcit in seborrheic keratosis, skin aging, and its clinical significance. Methods Immunohistochemistry was used to analyze the level of Hcit in skin lesions of seborrheic keratosis (SK), unaffected skin (distant 0.5 centimeters from SK lesion), and normal skin of healthy subjects in the control group. ELISA test was used to detect the serum level of CBL in SK patients and healthy subjects of different ages. Results Hcit was mainly localized in the nucleus of epidermal cells. In healthy control skin, the expression of Hcit increased with age and showed a positive correlation with age (the correlation coefficient was 0.806, p = 0.0002). The expressional level of Hcit in SK lesions was higher than that in healthy control skin (Z = −3.703, p = 0.0002). The serum level of CBL in healthy subjects and in SK patients increased with age (the correlation coefficient were 0.5763, p = 0.0032; 0.682, p = 0.004. respectively). The serum level of CBL in SK patients was higher than that in healthy subjects (Z = −2.19, p = 0.030). Study limitations The small serum sample size in the study. Conclusion The high expressional level of Hcit is correlated with seborrheic keratosis and skin aging. HCit may be one of the potential biomarkers of skin aging. Hcit, , (Hcit) diseases However (CBL obscure aging significance SK, (SK) distant 05 0 5 0. lesion, lesion lesion) group ages cells 0806 806 0.806 0.0002. 00002 0.0002 . 0002 0.0002) Z 3703 3 703 −3.703 05763 5763 0.5763 0.0032 00032 0032 0682 682 0.682 0004 004 0.004 respectively. respectively respectively) 219 2 19 −2.19 0.030. 0030 0.030 030 0.030) study (Hcit (SK 080 80 0.80 0000 0.000 000 370 70 −3.70 0576 576 0.576 0.003 0003 003 068 68 0.68 00 0.00 21 1 −2.1 0.03 03 08 8 0.8 37 7 −3.7 057 57 0.57 06 6 0.6 0.0 −2. −3. −2 −3 −

ABSTRACT Introduction: According to the American Heart Association guideline for coronary artery bypass grafting (CABG), female patients undergoing on-pump CABG (ONCAB) are at higher risk of short-term adverse outcomes than male patients. However, whether off-pump CABG (OPCAB) can improve the short-term outcome of female patients compared to ONCAB remains unclear. Methods: We conducted a meta-analysis to study the effect of the female sex on short-term outcomes of OPCAB vs. ONCAB. A total of 31,115 patients were enrolled in 12 studies, including 20,245 females who underwent ONCAB and 10,910 females who underwent OPCAB. Results: The in-hospital mortality in female patients who underwent OPCAB was significantly lower than in those in the ONCAB group with (2.7% vs. 3.4%; odds ratio [OR] 0.76; 95% confidence interval [CI] 0.65-0.89) and without (OR 0.68; 95% CI 0.52-0.89) adjustment for cardiovascular risk factor. The incidence of postoperative stroke in female patients who underwent OPCAB was lower than in those in the ONCAB group (1.2% vs. 2.1%; OR 0.59; 95% CI 0.48-0.73) before cardiovascular risk factor adjustment but was not significant (OR 0.87; 95% CI 0,66-1.16) after adjustment. There was no significant difference in the incidence of postoperative myocardial infarction between women who underwent OPCAB and those in the ONCAB group (1.3% vs. 2.3%; OR 0.88; 95% CI 0.54-1.43). Conclusion: In contrast to the American Heart Association CABG guideline, female patients who had OPCAB don’t have unfavorable outcomes compared with the ONCAB group. Introduction CABG, , (CABG) onpump pump (ONCAB shortterm short term However offpump off (OPCAB unclear Methods metaanalysis meta analysis vs 31115 31 115 31,11 1 studies 20245 20 245 20,24 10910 10 910 10,91 Results inhospital hospital 2.7% 27 2 7 (2.7 3.4% 34 3 4 [OR 0.76 076 0 76 95 [CI 0.650.89 065089 0.65 0.89 65 89 0.65-0.89 0.68 068 68 0.520.89 052089 0.52 52 0.52-0.89 1.2% (1.2 2.1% 21 0.59 059 59 0.480.73 048073 0.48 0.73 48 73 0.48-0.73 0.87 087 87 0,661.16 066116 0,66 1.16 66 16 0,66-1.16 1.3% 13 (1.3 2.3% 23 0.88 088 88 0.541.43. 054143 0.54 1.43 . 54 43 0.54-1.43) Conclusion dont don t (CABG 3111 11 31,1 2024 24 20,2 1091 91 10,9 2.7 (2. 3.4 0.7 07 9 650 0.650.8 06508 065 0.6 089 0.8 6 8 0.65-0.8 06 520 0.520.8 05208 052 0.5 5 0.52-0.8 1.2 (1. 2.1 05 480 0.480.7 04807 048 0.4 073 0.48-0.7 08 661 0,661.1 06611 066 0,6 116 1.1 0,66-1.1 1.3 2.3 541 0.541.43 05414 054 143 1.4 0.54-1.43 311 31, 202 20, 109 10, 2. (2 3. 0. 0.650. 0650 0.65-0. 0.520. 0520 0.52-0. 1. (1 0.480. 0480 04 0.48-0. 0,661. 0661 0, 0,66-1. 0.541.4 0541 14 0.54-1.4 ( 0.650 0.65-0 0.520 0.52-0 0.480 0.48-0 0,661 0,66-1 0.541. 0.54-1. 0.65- 0.52- 0.48- 0,66- 0.541 0.54-1 0.54-

RESUMO Introdução: O treinamento em artes marciais concentra-se na ciência, na metodologia e na prática. As artes marciais são um símbolo de aptidão física. A análise acadêmica da influência do treinamento de artes marciais na saúde física das crianças é de grande importância para a promoção da cultura tradicional chinesa e pode enriquecer os programas de intervenção esportiva para melhorar a saúde física infantil. Objetivo: Analisar o efeito do treinamento de qualidade sensitiva no treinamento de equilíbrio das artes marciais em crianças. Métodos: Selecionou-se crianças de 8 a 12 anos como objetos de pesquisa. Após repetidas deliberações, foi elaborado e implementado um conjunto de artes marciais infantis. O projeto piloto analisa a importância da qualidade sensitiva na etapa essencial do treinamento das artes marciais infantis. Resultados: Após o experimento, houve uma diferença significativa na qualidade do equilíbrio no grupo experimental (p<0,01). Após o experimento, a diferença no sentido de equilíbrio entre os grupos experimental e de controle foi significativa (p<0,01). Conclusão: Na fase de treinamento básico das artes marciais infantis, os treinadores devem exigir que os atletas dominem as habilidades básicas das artes marciais de forma mais abrangente. Todas as combinações de movimentos nas artes marciais exigem aptidão física e sensibilidade de equilíbrio abrangentes, incluindo velocidade, flexibilidade e coordenação. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

ABSTRACT Introduction: Martial arts training focuses on science, methodology, and practice. Martial arts are a symbol of physical fitness. The academic analysis of the influence of martial arts training on children’s physical health is of great importance for promoting traditional Chinese culture. It can enrich sports intervention programs to improve children’s physical health. Objective: Analyze the effect of sensory quality training on martial arts balance training in children. Methods: Children aged 8 to 12 years were selected as research subjects. After repeated deliberations, a children’s martial arts set was designed and implemented. The pilot project analyzes the importance of sensory quality in the essential stage of children’s martial arts training. Results: After the experiment, there was a significant difference in balance quality in the experimental group (p<0.01). After the experiment, the difference in balance sense between the experimental and control groups was significant (p<0.01). Conclusion: In the basic training phase of children’s martial arts, coaches should require athletes to master basic martial arts skills more comprehensively. All movement combinations in martial arts require comprehensive physical fitness and balance sensitivity, including speed, flexibility, and coordination. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: El entrenamiento de las artes marciales se centra en la ciencia, la metodología y la práctica. Las artes marciales son un símbolo de la condición física. El análisis académico de la influencia del entrenamiento de artes marciales en la salud física de los niños es de gran importancia para la promoción de la cultura tradicional china y puede enriquecer los programas de intervención deportiva para mejorar la salud física de los niños. Objetivo: Analizar el efecto del entrenamiento de la calidad sensorial en el entrenamiento del equilibrio en artes marciales en niños. Métodos: Se seleccionaron niños de 8 a 12 años como sujetos de la investigación. Tras repetidas deliberaciones, se diseñó y puso en marcha un conjunto de artes marciales para niños. El proyecto piloto analiza la importancia de la calidad sensorial en la etapa esencial del entrenamiento de artes marciales de los niños. Resultados: Después del experimento, hubo una diferencia significativa en la calidad del equilibrio en el grupo experimental (p<0,01). Tras el experimento, la diferencia en el sentido del equilibrio entre los grupos experimental y de control fue significativa (p<0,01). Conclusión: En la fase de formación básica de las artes marciales infantiles, los entrenadores deberían exigir a los deportistas un dominio más completo de las habilidades marciales básicas. Todas las combinaciones de movimientos en las artes marciales requieren una amplia aptitud física y sensibilidad al equilibrio, incluidas la velocidad, la flexibilidad y la coordinación. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Abstract This study aimed to explore the influences of cobalt-sourced irradiation on the meat quality and storage stability of Procambarus clarkii. Pieces of tail meat of P. clarkii were processed, boiled, and packed in transparentretort pouchs and then exposed to 60Co-γ-ray (0, 1.25, 3.32, 5.30, and 7.24 kGy). Changes in meat indices such as pH, total plate count, and total volatile base-nitrogen (TVB-N), texture properties, were investigated. After irradiation at the optimum irradiation dose, crayfish meats was stored at 0, 4 and 8 °C respectively, samples were collected for determinations at 0, 3, 6, 9 and 12 days. With increased irradiation dose, the total plate count in meat significantly decreased, whereas TVB-N greatly increased (P < 0.05). The irradiation dose of 3.32 kGy can realize both sterilization effect and maintain the quality of crayfish meat to the maximum extent. Total plate count and TVB-N in crayfish meat slowly grew at a storage temperature of 0 °C, their contents reached or approached the edible threshold by 9th day of storage, thereby prolonging the shelf life to 9d. Research demonstrates that 3.32 kGy cobalt-sourced irradiation and 0 °C storage can provide some bacteriostasis to some extent and prolong the shelf life of boiled crayfish.

Abstract Objectives: The Lateral Arm Free Flap (LAFF) offers advantageous features like variable thickness and minimal hair growth. LAFF is primarily used in orofacial and pharyngeal reconstructions, with limited reports on tracheal/esophageal applications. Also, the psychological prognosis and quality of life in patients with LAFF reconstruction are lacking. Methods: This retrospective case series presents outcomes of LAFF reconstruction for head and neck surgical defects which included 19 patients. The disease type, location of reconstruction, TNM staging, tumor size, chemoradiation status, anxiety, depression, quality of life and detailed perioperative information of the LAFF reconstruction surgery were presented in a descriptive pattern. Subgroup analysis was conducted using Mann–Whitney U and Pearson r test Conclusions: The lateral arm free flap exhibits remarkable versatility and multifunctionality, providing advantageous outcomes in head and neck reconstruction. Level of evidence: Level 4 according to OCEBM. Objectives (LAFF growth reconstructions trachealesophageal tracheal esophageal applications Also lacking Methods 1 type staging size status anxiety depression pattern MannWhitney Mann Whitney Conclusions multifunctionality evidence OCEBM

Abstract Objective Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been fully elucidated. To address this, we have investigated the probable anti-inflammatory mechanism of W54011 in LPS-treated inflammation in human gingival fibroblasts (HGFs). Methodology HGFs were isolated from healthy gingival tissue samples using the tissue block method and were identified with immunofluorescence staining. The CCK8 assay and reverse transcription-PCR (RT-PCR) were used to select the optimal induction conditions for Lipopolysaccharide (LPS) and C5aRA (according to supplementary data S1, S2 and S3). The levels of inflammatory cytokines, C5aR, and the activation of NF-κB/MAPK signaling pathways were determined by RT-quantitative PCR (RT-qPCR) and Western blotting. Results Immunofluorescence results showed that vimentin and FSP-1 were positive in HGFs and Keratin was negative in HGFs. Immunofluorescence staining demonstrated that C5aRA inhibited LPS-stimulated nuclear translocation of p-p65. RT-qPCR and Western blotting showed that C5aRA reduced the expression of IL-1β, IL-6, TNF-α, C5aR, p-p65, p-IκBα, p-JNK, p-c-JUN, and TLR4 in LPS-induced HGFs. Conclusion These findings suggested that C5aRA attenuated the release of inflammatory cytokines in LPS-induced HGFs by blocking the activation of the NF-κB and MAPK signaling pathways. W W5401 nonpeptide non peptide CaR C aR CaRA aRA (C5aRA antiinflammatory anti models However elucidated this LPStreated LPS treated . (HGFs) CCK transcriptionPCR transcription RTPCR RT (RT-PCR (LPS according S1 S S3. S3 S3) NFκB/MAPK NFκBMAPK NF κB/MAPK κB RTquantitative quantitative RTqPCR qPCR (RT-qPCR FSP1 FSP 1 FSP- LPSstimulated stimulated pp65. pp65 pp p p65. p65 p-p65 IL1β, IL1β ILβ IL 1β, 1β β IL-1β IL6, IL6 6, 6 IL-6 TNFα, TNFα TNF α, α TNF-α pp65, p65, pIκBα, pIκBα IκBα, IκBα p-IκBα pJNK, pJNK JNK, JNK p-JNK pcJUN, pcJUN c JUN, JUN p-c-JUN TLR LPSinduced induced NFκB W540 (HGFs κBMAPK pp6 p6 p-p6 IL- W54 p-p W5