Resumo Antecedentes Os distúrbios da marcha são sintomas prevalentes e debilitantes, diminuindo muito a mobilidade e a qualidade de vida dos indivíduos com doença de Parkinson (DP). Embora os tratamentos tradicionais ofereçam alívio parcial, há um interesse crescente em intervenções alternativas para enfrentar esse desafio. Recentemente, um aumento notável no desenvolvimento de tecnologia assistida (TA) foi testemunhado para ajudar indivíduos com DP. Objetivo Explorar o cenário crescente de intervenções de TA adaptadas para aliviar deficiências de marcha relacionadas à DP e descrever as pesquisas atuais para esse fim. Métodos Nessa revisão, pesquisamos artigos em inglês publicados no PubMed de 2018 a 2023. Além disso, os resumos de cada trabalho foram lidos para assegurar a sua inclusão. Quatro pesquisadores buscaram independentemente os artigos de acordo com os critérios de inclusão e exclusão. Resultados Incluímos trabalhos que preencheram os critérios de inclusão. Identificamos as tendências em tecnologia assistiva na análise dos parâmetros da marcha em DP. Esses compreendem os sensores portáteis, análise da marcha, retroalimentação em tempo real e técnicas de pista, realidade virtual e robótica. Conclusão Essa revisão é um recurso para orientar pesquisas futuras, informar decisões clínicas e promover a colaboração entre pesquisadores, médicos e formuladores de políticas. Ao delinear os contornos deste campo em rápida evolução, pretende inspirar mais inovação, melhorando em última análise a vida dos pacientes com DP através de intervenções mais eficazes e personalizadas. debilitantes . (DP) parcial desafio Recentemente (TA fim 201 2023 disso exclusão portáteis pista robótica futuras políticas evolução inovação personalizadas (DP 20 202 2

Abstract Background Gait disturbances are prevalent and debilitating symptoms, diminishing mobility and quality of life for Parkinson's disease (PD) individuals. While traditional treatments offer partial relief, there is a growing interest in alternative interventions to address this challenge. Recently, a remarkable surge in assisted technology (AT) development was witnessed to aid individuals with PD. Objective To explore the burgeoning landscape of AT interventions tailored to alleviate PD-related gait impairments and describe current research related to such aim. Methods In this review, we searched on PubMed for papers published in English (2018-2023). Additionally, the abstract of each study was read to ensure inclusion. Four researchers searched independently, including studies according to our inclusion and exclusion criteria. Results We included studies that met all inclusion criteria. We identified key trends in assistive technology of gait parameters analysis in PD. These encompass wearable sensors, gait analysis, real-time feedback and cueing techniques, virtual reality, and robotics. Conclusion This review provides a resource for guiding future research, informing clinical decisions, and fostering collaboration among researchers, clinicians, and policymakers. By delineating this rapidly evolving field's contours, it aims to inspire further innovation, ultimately improving the lives of PD patients through more effective and personalized interventions. symptoms Parkinsons Parkinson s (PD relief challenge Recently (AT PDrelated aim 20182023. 20182023 2018 2023 . (2018-2023) Additionally independently criteria sensors realtime real time techniques reality robotics decisions clinicians policymakers fields field contours innovation 2018202 201 202 (2018-2023 201820 20 (2018-202 20182 2 (2018-20 (2018-2 (2018- (2018 (201 (20 (2 (

ABSTRACT Purpose: The continuous improvement and development of fertility care, internationally, requires ongoing monitoring of current delivery processes and outcomes in clinical practice. This descriptive and exploratory mixed-methods study was conducted in eight countries (Brazil, China, France, Germany, Italy, Mexico, Spain and the United Kingdom) to assess the unmet needs of fertility patients (male and female), and existing challenges, barriers and educational gaps of physicians and laboratory specialists involved in human fertility care during the COVID-19 pandemic. Materials and Methods: The study was deployed sequentially in two phases: 1) in-depth 45-minute semi-structured interviews (n=76), transcribed, coded and thematically analysed using an inductive reasoning approach, 2) an online survey (n=303) informed by the findings of the qualitative interviews, face validated by experts in reproductive medicine, and analysed using descriptive and inferential statistical methods. Results: The integrated results of both phases indicated numerous areas of challenges, including: 1) investigating male-related infertility; 2) deciding appropriate treatment for men and selective use of assisted reproductive technology; and 3) maintaining access to high-quality fertility care during a pandemic. Conclusions: The paper presents a reflective piece on knowledge and skills that warrant ongoing monitoring and improvement amongst reproductive medicine healthcare professionals amidst future pandemics and unanticipated health system disruptions. Moreover, these findings suggest that there is an additional need to better understand the required changes in policies and organizational processes that would facilitate access to andrology services for male infertility and specialized care, as needed. Purpose internationally practice mixedmethods mixed methods Brazil, Brazil (Brazil China France Germany Italy Mexico Kingdom female, female , female) challenges COVID19 COVID 19 COVID-1 pandemic Methods 1 indepth depth 45minute minute 45 semistructured semi structured n=76, n76 n n=76 76 (n=76) transcribed approach 2 n=303 n303 303 (n=303 Results including malerelated related technology 3 highquality high quality Conclusions disruptions Moreover needed COVID1 COVID- 4 n7 n=7 7 (n=76 n=30 n30 30 (n=30 n= (n=7 n=3 n3 (n=3 (n= (n

Objectives: The present study analyzed the reciprocal relationships between four common pediatric ophthalmic diseases (i.e., hyperopia, myopia, astigmatism, and strabismus) and attention‐deficit/hyperactivity disorder (ADHD) in children. Methods: This study enrolled 86,028 children with ADHD and 1,798,673 children without ADHD in the Taiwan Maternal and Child Health Database who were born at any time from 2004 to 2017. Cox proportional hazards regression models were used to estimate the bidirectional relationships of the four ophthalmic diseases with ADHD in children after adjusting for age, sex, and gestational age at birth. Survival curves for time-to-event variables were estimated using the Kaplan-Meier method, and the log-rank test was used to compare the curves. Results: The results indicated that ADHD significantly predicted the occurrence of hyperopia, myopia, astigmatism, and strabismus. Furthermore, hyperopia, myopia, astigmatism, and strabismus significantly predicted the occurrence of ADHD. The time between enrollment and ADHD diagnosis was shorter for patients with ophthalmic diseases than for the control group, and the time between enrollment and ophthalmic disease diagnosis was also shorter for ADHD patients than for the control group. Sex differences were found in the associations between ADHD and ophthalmic diseases. Conclusion: Clinicians should monitor children with ADHD for hyperopia, myopia, astigmatism, and strabismus to ensure appropriate treatment, and vice versa. Objectives i.e., ie i e (i.e. hyperopia myopia astigmatism attentiondeficithyperactivity attention deficit hyperactivity (ADHD Methods 86028 86 028 86,02 1798673 1 798 673 1,798,67 200 2017 sex birth timetoevent event KaplanMeier Kaplan Meier method logrank log rank Results Furthermore group Conclusion treatment versa i.e. (i.e 8602 8 02 86,0 179867 79 67 1,798,6 20 201 i.e 860 0 86, 17986 7 6 1,798, 2 1798 1,798 179 1,79 17 1,7 1,

ABSTRACT Introduction: The remission induction treatment for acute myeloid leukemia (AML) has remained unchanged in the resource-limited setting in the Philippines. AML treatment consists of induction chemotherapy followed by high dose consolidation chemotherapy or allogeneic hematopoietic stem cell transplantation. In the Philippines, the Filipino household bears the burden of health care cost of hospitalization expenditure. Insights into the treatment costs becomes an essential requirement as these guides the allocation of resources to scheme health programs. Method: This study involved a retrospective cohort analysis of AML patients who underwent treatment for AML. Review of the statements of account per admission per patient during treatment for remission induction, consolidation, relapsed and refractory disease and best supportive care from 2017 to 2019. Of the 251 eligible patients, 190 patients were included. Result: The mean healthcare expenditure for remission induction chemotherapy (Phase 1) was US $2, 504.78 (Php 125,239.29). While 3 to 4 cycles of consolidation chemotherapy cost an average of US $3,222.72 (Php 162,103.20). For patients who had relapsed and refractory disease, an additional mean cost of US $3,163.32 (Php 159,115.28) and US $2, 914.72 (Php 146,610.55) were incurred, respectively. The average cost of palliative care was US $1,687.00 (Php 84,856.59). Conclusion: The cost of chemotherapy and other therapeutics bear most of the weight of the direct healthcare cost. The cost of AML treatment represents a significant economic burden for patients and the institution. The cost increases as patients proceed through subsequent lines of treatment for induction failure. Existing subsidy for health insurance benefits could still be improved for appropriate source allocation of resources. Introduction (AML resourcelimited resource limited Philippines transplantation programs Method 201 2019 25 19 included Result Phase 1 2 $2 50478 504 78 504.7 Php 125,239.29. 12523929 125,239.29 . 125 239 29 125,239.29) 322272 222 72 $3,222.7 162,103.20. 16210320 162,103.20 162 103 20 162,103.20) 316332 163 32 $3,163.3 159,115.28 15911528 159 115 28 91472 914 914.7 146,610.55 14661055 146 610 55 incurred respectively 168700 687 00 $1,687.0 84,856.59. 8485659 84,856.59 84 856 59 84,856.59) Conclusion institution failure $ 5047 50 7 504. 1252392 125,239.2 12 23 32227 22 $3,222. 1621032 162,103.2 16 10 31633 $3,163. 159,115.2 1591152 15 11 9147 91 914. 146,610.5 1466105 14 61 5 16870 68 0 $1,687. 848565 84,856.5 8 85 125239 125,239. 3222 $3,222 162103 162,103. 3163 $3,163 159,115. 159115 9 146,610. 146610 6 1687 $1,687 84856 84,856. 12523 125,239 322 $3,22 16210 162,103 316 $3,16 159,115 15911 146,610 14661 168 $1,68 8485 84,856 1252 125,23 $3,2 1621 162,10 31 $3,1 159,11 1591 146,61 1466 $1,6 848 84,85 125,2 $3, 162,1 159,1 146,6 $1, 84,8 125, $3 162, 159, 146, $1 84,

The objective of the current study was to evaluate the effects of dietary supplementation with chromium yeast (Cr-yeast) and dihydropyridine (DHP) on nutrient utilization, milk composition, and antioxidative status of prepartum dairy cows under heat stress. Twenty-four perinatal dairy cows (600 ± 14 kg; parity 2-3) were divided into three groups and fed the same basal diet with no supplement (control group), 8 g of Cr-yeast (Cr-yeast group), or 3 g of DHP (DHP group) per day. The experimental period consisted of a 7-d adaptation period and a 30-d data collection period from day 27 prepartum to day 3 postpartum. The temperature humidity index (THI) observed at daytime during the trial period exceeded 72, and the mean daily THI was 79.43. Norepinephrine in the Cr-yeast group decreased in prepartum cows. Serum cortisol in Cr-yeast or DHP group cows was lower in the postpartum period than in the control; however, serum cortisol levels decreased in the DHP group in the prepartum period. Dietary supplementation with Cr-yeast increased the digestibility of ether extract and the concentration of milk fat, while the solid non-fat (SNF) level was reduced. In the DHP group, the lactoprotein content was higher than the control. Total antioxidant capacity (T-AOC) was higher and the malondialdehyde (MDA) content tended to increase before calving in the Cr-yeast and DHP groups. Dietary supplementation with Cr-yeast or DHP can relieve the adverse impact caused by heat stress in prepartum dairy cows.

The current study aimed to assess the effects of the addition of dietary fermented feed (FF) on the growth performance, blood parameters, intestinal morphology, and number of cecal microorganisms of Sichuan white geese from 1 to 28 d of age. A total of 240, 1-day-old female geese were randomly divided into five groups with six replicates (eight birds per replicate). Geese in the control group were fed the basal diet (0.0% FF) and the experimental groups were fed the basal diet supplemented with 2.5%, 5.0%, 7.5%, and 10.0% FF, respectively. The 5.0% FF and 7.5% FF groups had greater body weights (BW), average daily feed intakes (ADFI), and average daily gains (ADG) than the control group. The feed/gain ratio (F/G) in the 7.5% FF group was higher than the control group. The concentrations of immunoglobulin A (IGA), superoxide dismutase (SOD), catalase, total antioxidant capacity (T-AOC), and glutathione peroxidase (GPx) in the 5.0% FF group were higher than those in the control group, whereas malondialdehyde (MDA) content in the 5.0% FF group was lower. Moreover, the FF did not affect the plasma biochemical indicators, number of cecal microorganisms, and intestinal morphology of geese in the groups. These findings indicate that 5.0% FF may improve the growth performance (BW, ADG, and ADFI), immunological function, and antioxidant capacity of Sichuan white geese from 1 to 28 days of age.

Abstract: Background and Aims: Cantharellus species are traded in agricultural markets of the Mountain Region, Veracruz, Mexico, as an appreciated mushroom. The objective of this study was to analyze nutrients and nutraceutical properties in four Cantharellus species from Veracruz: antioxidant activity, total phenols, flavonoids, ascorbic acid, lycopene, and β-carotene; as well as the nutritional properties: dry matter, total ashes, crude protein, crude fat, crude fiber, moisture, carbohydrates and energy value of four wild edible mushrooms (Cantharellus violaceovinosus, C. veraecrucis, C. roseocanus y Cantharellus sp.). Methods: Basidiomas of Cantharellus spp. were collected in the Mountain Region, Veracruz, Mexico. The ITS and tef-1α regions were amplified and sequenced. Species were identified molecularly based on the BLAST results. Samples were lyophilized and stored at 4 °C in vacuum bags for the preservation of nutraceutical and nutritional compounds. A methanol-water mixture (80:20 v/v) was used to extract the nutraceutical compounds and to analyze them by spectrophotometric techniques. Key results: In general, outstanding values were found in C. violaceovinosus both in crude protein content (8.04 g/100 g-1) and in antioxidant capacity 2.28 mg TE/g extract. For flavonoids, C. roseocanus showed 2.98 mg QE/g extract and 6.23±0.68 mg/g of carotene. In addition, a high energy value was found in C. violaceovinosus (266.53 Kcal/100g) and C. roseocanus (222.73±15.43 Kcal/100 g). The nutritional and nutraceutical content of C. violaceovinosus, C. veraecrucis and C. roseocanus is presented for the first time. Conclusions: The results show these species from nutritional and nutraceutical perspectives. Cantharellus violaceovinosus showed the highest values of the parameters evaluated. The consumption of these species constitutes an alternative source of protein and nutraceutical components that contribute to food security.

Resumen: Antecedentes y Objetivos: Las especies de Cantharellus se comercializan en los mercados agrícolas de la Región de Las Montañas, Veracruz, México, como un hongo apreciado. El objetivo de este estudio fue analizar los nutrientes y propiedades nutracéuticas en cuatro especies de Cantharellus de Veracruz: capacidad antioxidante, fenoles totales, flavonoides, ácido ascórbico, licopeno y caroteno; así como las propiedades nutricionales: materia seca, cenizas totales, proteína bruta, grasa, fibra bruta, humedad, carbohidratos y valor energético de cuatro hongos silvestres comestibles (Cantharellus violaceovinosus, C. veraecrucis, C. roseocanus and Cantharellus sp.). Métodos: Se colectaron basidiomas de Cantharellus spp. en la Región de la Montaña, Veracruz, México. Se amplificaron las regiones ITS y tef-1α y secuenciaron. Las especies se identificaron molecularmente en base en los resultados de BLAST. Las muestras fueron liofilizadas y almacenadas a 4°C en bolsas al vacío para la conservación de compuestos nutracéuticos y nutricionales. Se utilizó una mezcla metanol-agua (80:20 v/v) para extraer los compuestos nutracéuticos y analizarlos por técnicas espectrofotométricas. Resultados clave: En general, se encontraron valores sobresalientes en C. violaceovinosus, tanto en contenido de proteína cruda (8.04 g/100 g-1) como en capacidad antioxidante (2.28 mg TE/g extracto). Para los flavonoides, C. roseocanus mostró 2.98 mg QE/g de extracto y 6.23±0.68 mg de caroteno/g de extracto. Además, se encontró un alto valor energético en C. violaceovinosus (266.53 Kcal/100g) y C. roseocanus (222.73±15.43 Kcal/100g) (266.53±14.64 Kcal/100g). Se presenta por primera vez el contenido nutricional y nutracéutico de C. violaceovinosus, C. veraecrucis y C. roseocanus. Conclusiones: Los resultados muestran estas especies desde las perspectivas nutricional y nutracéutica. Cantharellus violaceovinosus mostró los valores más altos de los parámetros evaluados. El consumo de estas especies constituye una alternativa como fuentes de proteínas y componentes nutracéuticos que contribuyen a la seguridad alimentaria.

Abstract Introduction: The objective of this study was to investigate the effect of mivacurium in the application of fast-track anesthesia for transthoracic device closure of ventricular septal defects (VSDs) in children. Methods: The data of 108 children who underwent transthoracic device closure of VSDs from December 2018 to June 2020 were recorded and analyzed. All children were divided into group M (mivacurium group, n=55) and group C (cisatracurium group, n=53) according to the different muscle relaxant drug used. Results: No statistically significant differences in general preoperative data, intraoperative hemodynamic changes, or the incidence of adverse reactions were noted between the two groups (P>0.05). However, the intubation condition rating of children in group M was better than that in group C. The onset time, duration of clinical action and recovery index of the muscle relaxant, postoperative mechanical ventilation duration, and length of intensive care unit stay in group M were significantly lower than those in group C (P<0.05). Conclusion: It is safe and feasible to use mivacurium as a muscle relaxant in children undergoing fast-track cardiac anesthesia during transthoracic device closure of VSDs.

ABSTRACT Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI). Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed. Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics. Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.

Resumo O cádmio (Cd) é um dos principais tóxicos, que afeta a saúde humana por meio da exposição ocupacional e ambiental. No presente estudo, avaliamos os efeitos protetores dos cogumelos morel contra os danos reprodutivos induzidos pelo Cd em ratos. Para tanto, 30 ratos machos foram divididos em 6 grupos (n = 5 / grupo); o primeiro grupo serviu de controle, o segundo grupo foi tratado com injeção intraperitoneal (i.p) de 1 mg / kg / dia de Cd. O terceiro e o quarto grupos foram cotratados com 1 mg / kg / dia de Cd (i.p) e 10 e 20 mg / kg / dia de extrato de cogumelo morel (por via oral), respectivamente. Os dois grupos finais receberam gavagem oral de 10 e 20 mg / kg / dia de extrato de cogumelo morel sozinho. Após o tratamento por 17 dias, os animais foram sacrificados e os testículos e o epidídimo foram dissecados. Um testículo e epidídimo de cada animal foram processados para histologia, enquanto o outro testículo e epidídimo foram usados para produção diária de esperma (DSP) e ensaio cometa. Nossos resultados mostraram que os cogumelos Cd e morel não têm efeito sobre o peso do animal, mas o Cd diminui significativamente a contagem de DSP e danifica o DNA hereditário, que é revertido em grupos de cotratamento. Da mesma forma, os resultados histopatológicos dos testículos e do epidídimo mostram que os cogumelos morel controlam os danos a esses tecidos. Considerando que o extrato de cogumelo morel sozinho pode aumentar a produção de testosterona. Esses resultados concluem que os cogumelos morel não apenas controlam os danos causados pelo Cd, mas também podem ser usados como um mecanismo de proteção para danos hereditários ao DNA.

Abstract Cadmium (Cd) is one of the major toxicants, which affects human health through occupational and environmental exposure. In the current study, we evaluated the protective effects of morel mushrooms against Cd-induced reproductive damages in rats. For this purpose, 30 male rats were divided into 6 groups (n=5/group), the first group served as the control group, second group was treated with an intraperitoneal (i.p) injection of 1 mg/kg/day of Cd. Third and fourth groups were co-treated with 1 mg/kg/day of Cd (i.p) and 10 and 20 mg/kg/day of morel mushroom extract (orally) respectively. The final 2 groups received oral gavage of 10 and 20 mg/kg/day of morel mushroom extract alone. After treatment for 17 days, the animals were euthanized, and testes and epididymis were dissected out. One testis and epididymis of each animal were processed for histology, while the other testis and epididymis were used for daily sperm production (DSP) and comet assay. Our results showed that Cd and morel mushrooms have no effect on animal weight, but Cd significantly decreases the DSP count and damages the heritable DNA which is reversed in co-treatment groups. Similarly, the histopathological results of testes and epididymis show that morel mushrooms control the damage to these tissues. Whereas the morel mushroom extract alone could enhance the production of testosterone. These results conclude that morel mushrooms not only control the damage done by Cd, but it could also be used as a protection mechanism for heritable DNA damage.

Abstract The Clerodendranthus spicatus (Thunb.) C. Y. Wu extract (CSTCYWE) was administered to D-galactose-induced aging mice to investigate the effect and mechanism of CSTCYWE on exercise endurance in aging mice. The effect of CSTCYWE on the improvement of exercise capacity in aging mice was evaluated by detecting serum biochemical indicators, pathological changes in tissues, and expression by qPCR. The composition of CSTCYWE was analyzed by HPLC. The experiment showed that CSTCYWE increased the running time and the exhaustive swimming time of aging mice to improve their exercise endurance. CSTCYWE also reduced the levels of BUN, BLA, and MDA and increased the levels of HG, MG, SOD, and GSH-Px in the serum of aging mice. Pathological observation found that CSTCYWE alleviated the liver and kidney tissue lesions and damage caused by aging. Meanwhile, CSTCYWE could down-regulate the relative mRNA expressions of nNOS, iNOS, TNF-α, and syncytin-1 and up-regulate the relative mRNA expressions of Cu/Zn-SOD, Mn-SOD, and CAT in the mouse liver tissue and skeletal muscle tissue. The results of component analysis showed that CSTCYWE contained caffeic acid, hypericin, isoquercetin, dihydroquercetin, rosmarinic acid, baicalin, luteolin, and baicalein. These active ingredients enable CSTCYWE to act on aging mice and improve their exercise capacity.

Objective: The purpose of this study was to investigate the lifetime suicide attempt rate, clinical characteristics and cognitive function of Chinese patients with chronic schizophrenia who had attempted suicide. Methods: We collected data from 908 schizophrenia inpatients about suicide attempts through interviews with the patients and their families, as well as through medical records. All patients were assessed with the Positive and Negative Syndrome Scale, the Rating Scale for Extrapyramidal Side Effects, the Abnormal Involuntary Movement Scale, and the Repeated Battery for the Assessment of Neuropsychological Status. Results: Of this sample, 97 (10.68%) had attempted suicide. Patients who had attempted suicide were younger, had longer illness duration, and more severe general psychopathology and depressive symptoms than those who had not. Logistic regression analysis confirmed that suicide attempts were correlated with age, smoking, and depression. No cognitive performance differences were observed between patients who had and had not attempted suicide. Conclusions: In China, patients with chronic schizophrenia may have a higher prevalence of lifetime suicide attempts than the general population. Some demographic and clinical variables were related to suicide attempts in patients with chronic schizophrenia.

Ischemia-reperfusion injury (IRI) has brought attention to flap failure in reconstructive surgery. To improve the prognosis of skin transplantation, we performed experimental IRI by surgical obstruction of blood flow and used sodium ferulate (SF) to prevent IRI in rats. After SF treatment, the morphological and histological changes of the skin flaps were observed by H&E and Masson's trichrome staining. We also detected the expression levels of COX-1, HO-1, and Ki67 by immunohistochemical and western blot analysis. Moreover, enzyme-linked immunosorbent assay was used to identify the content of tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), malondialdehyde (MDA), and nitric oxide (NO) in peripheral blood and skin tissue. Compared with the model group, SF treatment significantly improved the recovered flap area (%) and promoted collagen synthesis. Cyclooxygenase-2 (COX-2) expression was significantly inhibited by heme oxygenase-1 (HO-1) induction after SF treatment. Furthermore, SF significantly inhibited the levels of TNF-α in peripheral blood, MPO and MDA in the skin tissue, and the increased synthesis of NO. Our results showed the protective effects of SF on IRI after flap transplantation and we believe that the protective effects of SF was closely related to the alleviation of the inflammatory response and the inhibition of the oxidative stress injury.

Abstract Until now, custom-made or commercial polyclonal antibody against only one kind of fish IgM limited application of the antibody. During our research on development of vaccine against infection of Clonorchis sinensis (C. sinensis) in several kinds of fish, we were conscious of the urgency of secondary antibody to evaluate immune effect and screen C. sinensis infection with immunological technology instead of labor-intensive and time-consuming squash or artificial digestion of fish flesh. So that, we purified IgM of grass carp, bighead carp, crucian carp, common carp and tilapia which were widely cultured freshwater fishes in most areas of China. On this basis, we generated HRP-conjunct rabbit IgG anti-fish IgMs with high titers. IgM of other freshwater fishes including oshima, yellow catfish, bream, silver carp and so on could be recognized by the IgG sensitively. Additionally, The ELISA detection displayed that the IgG could be more specific and sensitive than custom-made rabbit IgG anti-grass carp IgM. The acquirement of HRP-conjunct rabbit IgG anti-fish IgMs was the cornerstone for studying the immune system of teleost fish, developing immunoassay methods and evaluation of fish vaccine with more convenience.