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Acute toxicity study of bioactive galactomannans from seeds of two nontraditional leguminosae
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Marques, Fabrícia da C. J.
; Nascimento, Francisco G. da S.
; Nonato, Dayanne T. T.
; Assreuy, Ana M. S.
; Chaves, Edna M. C.
; Aragão, Gislei F.
; Soares, Pedro M. G.
; Castro, Rondinelle R.
.
RESUMO Fontes não tradicionais de galactomananas são amplamente cultivadas na Região Nordeste do Brasil, como as sementes das espécies ornamentais Caesalpinia pulcherrima (flor-depavão) e Delonix regia (flamboiã). As galactomananas GM-CP e GM -DR, extraídas respectivamente dessas sementes, estão sendo estudadas como potenciais agentes terapêuticos, mas avaliações sistemáticas sobre a toxicidade aguda das mesmas ainda não foram relatadas. Grupos de três ratas receberam GM-CP ou GM-DR oral (300 mg kg-1), enquanto um grupo controle recebeu veículo (solução salina). Como não houve letalidade, outros grupos receberam doses de 2000 mg kg-1, o que também não causou letalidade. A coleta de órgãos e amostras de sangue foi feita no dia 14. A hipernocicepção mecânica e o influxo de células inflamatórias foram medidos em outros grupos que receberam doses intra-articulares de GM-CP ou GM-DR (200 µg, n = 5 por grupo). Ambas as galactomananas não evocaram alterações fisiológicas/comportamentais ou inflamação articular. Como a DL50 não foi inferior a 2000 mg kg-1, tais polissacarídeos podem ser alocados na classe 5 do Sistema Globalmente Harmonizado para Classificação e Rotulagem de Produtos Químicos. Brasil flordepavão flor depavão (flor-depavão flamboiã. flamboiã . (flamboiã) GMCP CP DR, DR -DR terapêuticos relatadas GMDR 300 (30 kg1, kg1 kg 1 , kg-1) solução salina. salina salina) letalidade 200 1, kg-1 14 intraarticulares intra articulares (20 µg grupo. grupo) fisiológicascomportamentais fisiológicas comportamentais articular DL DL5 Químicos (flamboiã 30 (3 20 kg- (2 3 ( 2
ABSTRACT Non-traditional galactomannans sources are widely cultivated in the Brazilian Northeast Region, such as the species Caesalpinia pulcherrima (peacock flower) and Delonix regia (flamboyant). The galactomannans GM-CP and GM-DR were extracted from the respective seeds and are being studied as potential therapeutic agents, but systematic evaluations on their acute toxicity are yet to be reported. Groups of three female rats received oral GMCP or GM-DR (300 mg kg-1), whereas a control group received vehicle (saline). Since there was no lethality, other groups received doses of 2000 mg kg-1, which also did not cause lethality. Organs and blood samples were collected on day 14. Mechanical hypernociception and inflammatory cell influx were measured in other groups receiving intra-articular doses of GM-CP or GM-DR (200 µg, n = 5 per group). Neither galactomannan evoked physiological / behavioral changes or joint inflammation. Since the LD50 was less than 2000 mg kg-1, such polysaccharides may be allocated in the class 5 of the Globally Harmonized System for Classification and Labelling of Chemicals. Nontraditional Non traditional Region peacock flower flamboyant. flamboyant . (flamboyant) GM CP GMDR DR agents reported 300 (30 kg1, kg1 kg 1 , kg-1) saline. saline (saline) lethality 200 1, kg-1 14 intraarticular intra articular (20 µg group. group) inflammation LD LD5 Chemicals (flamboyant 30 (3 (saline 20 kg- (2 3 ( 2
2.
Impact of Conservation Processes on the Lipid Profile and Immunological Factors IL-10 and TGF-β1 in Whey Separated from Discarded Human Milk IL10 IL 10 IL-1 TGFβ1 TGFβ TGF β1 β TGF-β IL1 1 IL-
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Alves, Eloize S.
; Castro, Matheus C.
; Saqueti, Bruno H. F.
; Manin, Luciana P.
; Alencar, Josiane B.
; Zacarias, Joana M. V.
; Bruni, Andressa R. S.
; Madrona, Grasiele S.
; Visentainer, Jeane E. L.
; Cristianini, Marcelo
; Santos, Oscar O.
; Visentainer, Jesui V.
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
Reuse made by the separation of whey can reduce the waste of human milk. However, the authors are not aware of the literature on treatments in human whey, made available by pasteurization holder, freeze-drying, spray drying, and high hydrostatic pressure. In this context, the present study applied treatments to human whey and evaluated their lipid and immunological quality. Among the results, a different formation in the triacylglycerol profile was evidenced after the application of spray drying and high hydrostatic pressure, while pasteurization and freeze-drying presented ion intensity close to the control human whey. In addition, pasteurization proved to be adequate for lipid nutritional quality and transformation factor-β1 (TGF-β1) concentration, while an increase in interleukin-10 (IL-10) levels was promoted, between 73 and 80%, after freeze-drying, spray drying, and high hydrostatic pressure. Through the principal component analysis, it is noteworthy that the processes presented divergences in terms of the effects caused, with similarity only between pasteurization and freeze-drying in the composition of fatty acids. However, it was observed that all processes were able to maintain the nutrients. Nevertheless, it is relevant to consider individual characteristics presented and the interest in the desired quality, which can be promising as a complementary product to infant feeding. milk However holder freezedrying, freezedrying freeze pressure context results addition factorβ1 factorβ factor β1 β factor-β TGFβ1 TGFβ TGF (TGF-β1 concentration interleukin10 interleukin 10 interleukin-1 IL10 IL (IL-10 promoted 7 80 80% analysis caused acids nutrients Nevertheless feeding (TGF-β interleukin1 1 interleukin- IL1 (IL-1 8 (IL- (IL
3.
Poekilloptera phalaenoides (Hemiptera: Flatidae): occurrence on Inga striata (Fabaceae) in a mountainous region
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Pinheiro, R. A.
; Cabral, M. J. S.
; Giordani, S. C. O.
; Santos, C. A.
; Plata-Rueda, A.
; Castro, B. M. C.
; Zanuncio, J. C.
; Silva, W. M.
; Soares, M. A.
.
4.
Dopamine depletion in wistar rats with epilepsy
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Trindade-Filho, E. M.
; Pai, J. Dal
; Castro, D. N. de
; Silva, A. T. M. da
; Costa, A. F.
; Vieira, J. S. S.
; Santos, S. D. B. dos
; Félix, V. B.
; Leão, S. A. B. F.
; Zambrano, L. I.
; Saldanha-Filho, A. J. M.
; Carvalho, E. G. A.
; Cavalcante, J. B. N.
; Quintella, G. B.
; Lino, A. T. S.
; Costa, M. V.
; Lima, J. A.
; Tavares, M. M. A.
; de Melo, M. R.
; Mousinho, K. C.
; Biase, C. L. C. L. De
; Leite, M. L.
; Costa, P. J. M. S.
; Becker, E. L.
; Moura, I. M. F. B.
; Silva, J. C. da
.
Resumo O conteúdo de dopamina nas estruturas cerebrais tem sido relacionado à excitabilidade neuronal e várias abordagens têm sido utilizadas para estudar este fenômeno durante o período de vulnerabilidade às crises. No presente trabalho, descrevemos os efeitos da depleção de dopamina após a administração de 6-hidroxidopamina (6-OHDA) na região pars compacta da substância negra de ratos submetidos ao modelo de epilepsia com pilocarpina. A susceptibilidade ao estado de mal epiléptico induzido pela pilocarpina, bem como a frequência de crises espontâneas e recorrentes durante o período crônico do modelo foi determinada. Sendo o hipocampo uma das principais estruturas afetadas no desenvolvimento desse modelo experimental de epilepsia, os níveis de dopamina nessa região foram determinados após a administração da droga. No primeiro experimento, 62% (15/24) dos ratos pré-tratados com 6-OHDA e 45% (11/24) daqueles que receberam ácido ascórbico como solução controle evoluíram para crises límbicas motoras e para o estado de mal epiléptico, após a administração de pilocarpina. A gravidade das crises durante o período agudo do modelo foi significativamente maior nos ratos epilépticos experimentais (56,52%) do que nos ratos controle (4,16%). No segundo experimento, não houve diferença significante entre os grupos quanto à frequência de crises na fase crônica do modelo. Nossos dados mostraram que a dopamina pode desempenhar um papel importante na gravidade das crises na fase aguda da pilo, o que parece ser exercido por sua ação inibitória da dopamina sobre a expressão motora das crises.
Abstract The dopamine content in cerebral structures has been related to neuronal excitability and several approaches have been used to study this phenomenon during seizure vulnerability period. In the present work, we describe the effects of dopamine depletion after the administration of 6-hidroxidopamine (6-OHDA) into the substantia nigra pars compacta of male rats submitted to the pilocarpine model of epilepsy. Susceptibility to pilocarpine-induced status epilepticus (SE), as well as spontaneous and recurrent seizures (SRSs) frequency during the chronic period of the model were determined. Since the hippocampus is one of main structures in the development of this experimental model of epilepsy, the dopamine levels in this region were also determined after drug administration. In the first experiment, 62% (15/24) of 6-OHDA pre-treated rats and 45% (11/24) of those receiving ascorbic acid as control solution progressed to motor limbic seizures evolving to SE, after the administration of pilocarpine. Severeness of seizures during the model´s the acute period, was significantly higher in epileptic experimental rats (56.52%), than in controls (4.16%). In the second experiment, the frequency of seizures in the model’s chronic phase did not significantly change between groups. Our data show that dopamine may play an important role on seizure severity in the pilo’s model acute period, which seems to be due to dopamine inhibitory action on motor expression of seizure.
5.
Protonectarina sylveirae (Hymenoptera: Vespidae): first report preying Bedellia somnulentella (Lepidoptera: Bedelliidae) in Brazil
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Cabral, M. J. S.
; Silva, I. M.
; Pinheiro, R. A.
; Santos, M. M.
; Soares, M. A.
; Plata-Rueda, A.
; Castro, B. M. C.
; Silva, W. M.
; Silva, E. S.
; Zanuncio, J. C.
.
6.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
; Pereira, Thalles P.L.
; Zacca, Thamara
; Pacheco, Thaynara L.
; Martins, Thiago F.
; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
; Porto, Tiago J.
; Krolow, Tiago K.
; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
; Pinheiro, Ulisses dos S.
; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
; Costa, Valmir A.
; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
7.
Coleoptera of Brazil: what we knew then and what we know now. Insights from the Catálogo Taxonômico da Fauna do Brasil Brazil now
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Caron, Edilson
; Monné, Marcela L.
; Ferreira, Vinicius S.
; Costa, Cleide
; Cupello, Mario
; Aloquio, Sergio
; Linzmeier, Adelita M.
; Vaz-de-Mello, Fernando Z.
; Leivas, Fernando W.T.
; Souza-Gonçalves, Igor
; Mermudes, José R.M.
; Almeida, Lúcia M.
; Moura, Luciano de A.
; Ferreira Júnior, Nelson
; Grossi, Paschoal C.
; Vanin, Sergio A.
; Ślipiński, Adam
; Anichtchenko, Alexander
; Newton, Alfred F.
; Sampaio, Aline
; Carelli, Allan
; Puker, Anderson
; Ferreira, André da S.
; Fernandes, André S.
; Roza, André S.
; Cline, Andrew
; Sampaio, Brunno H.L.
; Clarkson, Bruno
; Castro, Camila F. de
; Bicho, Carla de L.
; Benetti, César J.
; Ribeiro-Costa, Cibele S.
; Lopes-Andrade, Cristiano
; Manfio, Daiara
; Colpani, Daniara
; Basílio, Daniel S.
; Bená, Daniela de C.
; Pollock, Darren A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Chandler, Donald S.
; Nascimento, Elynton A. do
; Spiessberger, Erich L.
; Agrain, Federico A.
; Barbosa, Felipe F.
; Shockley, Floyd
; Nascimento, Francisco E. de L.
; Biffi, Gabriel
; Powell, Gareth S.
; Morse, Geoffrey E.
; Flores, Gustavo E.
; Escalona, Hermes
; Quintino, Hingrid Y.S.
; Rainho, Hugo L.
; Maddalena, Italo S.C.P.
; Hájek, Jiří
; McHugh, Joseph V.
; Botero, Juan P.
; Fuhrmann, Juares
; Churata-Salcedo, Julissa M.
; Vieira, Letícia M.
; Silveira, Luiz F.L. da
; Cruz, Luiza S. da
; Sekerka, Lukás
; Bologna, Marco A.
; Bevilaqua, Marcus V.O.
; Passos, Maria I.
; Chamorro, Maria L.
; Cherman, Mariana A.
; Bento, Matheus
; Gimmel, Matthew
; Segura, Melissa O.
; Ivie, Michael A.
; Thomas, Michael C.
; Monné, Miguel A.
; Lord, Nathan
; Hamada, Neusa
; Degallier, Nicolas
; Santos, Paula B. dos
; Duarte, Paulo R.M.
; Gnaspini, Pedro
; Bulirsch, Petr
; Regalin, Renato
; Leschen, Richard A.B.
; Constantin, Robert
; Corrêa, Rodrigo C.
; Gerstmeier, Roland
; Rosa, Simone P.
; Campos, Stéphanie V.N.
; Peck, Stewart B.
; Pacheco, Thaynara L.
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Grzymala, Traci L.
; Smith, Trevor R.
; Costa-Silva, Vinicius da
; Sandoval-Gómez, Vivian E.
; Sousa, Wesley O. de
; Tomaszewska, Wioletta
.
ABSTRACT In 2000, Cleide Costa published a paper presenting the state of knowledge of the Neotropical Coleopte ra, with a focus on the Brazilian fauna. Twenty-four years later, thanks to the development of the Coleoptera section of the Taxonomic Catalog of the Brazilian Fauna (CTFB - Catálogo Taxonômico da Fauna do Brasil) through the collaboration of 100 coleopterists from all over the globe, we can build on Costa’s work and present an updated overview of the state of knowledge of the beetles from Brazil. There are currently 35,699 species in 4,958 genera and 116 families known to occur in the country, including representatives of all extant suborders and superfamilies. Our data show that the Brazilian beetle fauna is the richest on the planet, concentrating 9% of the world species diversity, with some estimates accounting to up to 15% of the global total. The most diverse family in numbers of genera is Cerambycidae (1,056 genera), while in number of species it is Chrysomelidae (6,079 species). Conotrachelus Dejean, 1835 (Curculionidae) is the most species-rich genus, with 570 species. The French entomologist Maurice Pic is the author who has contributed the most to the naming of species recorded from Brazil, with 1,794 valid names in 36 families, whereas the Brazilians Ubirajara R. Martins and Maria Helena M. Galileo are the only ones among the top-ten authors to have named species in the 21st century. Currently, approximately 144 new species of Brazilian beetles are described each year, and this average is projected to increase in the next decade to 180 species per year, or about one new Brazilian beetle every two days. 2000 ra Twentyfour Twenty four later CTFB Brasil 10 globe Costas s Brazil 35699 35 699 35,69 4958 4 958 4,95 11 country superfamilies planet 9 diversity 15 total 1,056 1056 1 056 (1,05 genera, , genera) 6,079 6079 6 079 (6,07 . species) Dejean 183 Curculionidae (Curculionidae speciesrich rich genus 57 1794 794 1,79 3 R M topten top ten st century Currently 14 year 18 days 200 3569 69 35,6 495 95 4,9 1,05 105 05 (1,0 6,07 607 07 (6,0 5 179 79 1,7 20 356 35, 49 4, 1,0 0 (1, 6,0 60 (6, 17 7 1, 2 (1 6, (6 (
8.
Microorganisms as growth promoters of Acmella oleracea grown under different cultivation systems
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Ferreira, Thayná da C.
; Santos, Gleiciane R. dos
; Moraes, Alessandra J. G. de
; Santos, Fernando de S.
; Mendonça, Danielle P.
; Silva, Matheus Y. F. da
; Castro, Gledson L. S. de
; Batista, Telma F. V.
.
Revista Brasileira de Engenharia Agrícola e Ambiental
- Métricas do periódico
RESUMO Um dos grandes desafios a serem superados é manter e aumentar a produtividade de hortaliças, como o jambu (Acmella oleracea), utilizando processos naturais e organismos vivos que estimulem a produção vegetal, com menos resíduos tóxicos no meio ambiente. O objetivo deste estudo foi avaliar o crescimento de plantas de A.oleracea, por meio de respostas biométricas e fisiológicas, após a aplicação dos fungos entomopatogênicos Beauveria bassiana e Metarhizium anisopliae e do promotor de crescimento Trichoderma asperellum, sob sistemas de plantio protegido e não protegido, nas estações chuvosa e seca da Amazônia. Dois ensaios foram conduzidos simultaneamente, em blocos casualizados, em uma área comercial de agricultura periurbana no município de Ananindeua, Pará, Brasil, em canteiros suspensos protegidos e não protegidos. Os isolados fúngicos de M. anisopliae, B. bassiana e T. asperellum promoveram o crescimento em plantações de jambu, em sistemas de plantio protegido e desprotegido, em ambos os ciclos de cultivo. No entanto, M. anisopliae se igualou aos resultados obtidos com o promotor de crescimento T. asperellum e se destacou por favorecer maior desempenho em todas as variáveis de crescimento avaliadas, principalmente no sistema de cultivo protegido e no período chuvoso amazônico. Além disso, as plantas de A. oleracea apresentaram melhor crescimento em todos os tratamentos, em sistema de plantio protegido, nos dois períodos avaliados. Portanto, plantas de A. oleracea tratadas com fungos como M. ansiopliae, B. bassiana e T. asperellum apresentaram maior crescimento, biomassa e trocas gasosas. hortaliças Acmella oleracea, , oleracea) vegetal ambiente Aoleracea A A.oleracea fisiológicas Amazônia simultaneamente casualizados Ananindeua Pará Brasil M B T desprotegido entanto avaliadas amazônico disso tratamentos avaliados Portanto ansiopliae gasosas
ABSTRACT A great challenge to overcome is how to maintain and increase the productivity of vegetables, such as jambu (Acmella oleracea), by using natural processes and living organisms that stimulate plant production and release fewer toxic residues into the environment. The objective of this study was to evaluate the growth of A. oleracea, based on biometric, and physiological responses, following the application of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae and the growth promoter Trichoderma asperellum, under protected and unprotected planting systems, in the rainy and dry seasons of the Amazon. Two trials were conducted simultaneously, in randomized blocks, in a commercial area of peri-urban agriculture in the municipality of Ananindeua, Pará state, Brazil, in protected and unprotected hanging beds. Metarhizium anisopliae, B. bassiana, and T. asperellum isolates promoted growth in jambu in protected and unprotected plantation systems, in both crop cycles. Of note, M. anisopliae matched the results obtained with the growth promoter T. asperellum and stood out for favoring greater performance in all of the evaluated growth variables, especially in the protected cultivation system and in rainy season. In addition, A. oleracea grew better in all treatments in a protected planting system and in both evaluated periods. Therefore, A. oleracea treated with M. anisopliae, B. bassiana, or T. asperellum presented better growth, produced more biomass, and exhibited superior gas exchange. vegetables Acmella , oleracea) environment biometric responses systems Amazon simultaneously blocks periurban peri urban Ananindeua state Brazil beds B T cycles note M variables season addition periods Therefore biomass exchange
9.
Effects of blood and root-dentin cleaning on the porosity and bond strength of a collagen bioceramic material rootdentin root dentin
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Saltareli, F. M.
; Castro-Raucci, L. M. S.
; Miranda, C. E. S.
; Tavella-Silva, N. C
; Oliveira, I. R.
; Raucci-Neto, W.
.
Resumo As amostras de raízes bovinas foram divididas em: sem contaminação (N); contaminação sanguínea (P); contaminação sanguínea e limpeza com soro fisiológico (S), 2,5% NaOCl+soro fisiológico (Na) ou 2,5% NaOCl+17%EDTA+soro fisiológico (NaE) e preenchido com: agregado de trióxido mineral (MTA), cimento de aluminato de cálcio (C), ou C+colágeno (Ccol) (n=13). A porosidade do MTA foi menor que C (p=0,02) e maior que Ccol (p<0,001). P e NaE foram semelhantes (p=1,00), mas superiores aos demais grupos (p<0,001). A resistência de união do MTA foi semelhante ao Ccol (p=0,777) e inferior ao C (p=0,028). P apresentou menor resistência de união que o N (p<0,001); S e Na foram semelhantes entre si (p=0,969), porém maiores que P e menores que N (p<0,001). Este estudo mostrou que a contaminação com sangue aumentou a porosidade dos cimentos, mas a limpeza da dentina com NaOCl 2,5% reduziu esse efeito, e o aditivo de colágeno reduziu a porosidade dos cimentos. Além disso, a contaminação sanguínea reduziu a resistência de união dos cimentos e a limpeza com solução salina ou NaOCl 2,5% diminuiu esse efeito. em (N) (P) S, , (S) 25 2 5 2,5 NaOClsoro (Na NaOCl17EDTAsoro NaOClEDTAsoro 17 EDTA (NaE MTA, (MTA) C, (C) Ccolágeno (Ccol n=13. n13 n n=13 . 13 (n=13) p=0,02 p002 p 0 02 (p=0,02 p<0,001. p0001 p<0,001 001 (p<0,001) p=1,00, p100 p=1,00 1 00 (p=1,00) p=0,777 p0777 777 (p=0,777 p=0,028. p0028 p=0,028 028 (p=0,028) p=0,969, p0969 p=0,969 969 (p=0,969) efeito disso (N (P (S 2, EDTAsoro (MTA (C n1 n=1 (n=13 p=0,0 p00 (p=0,0 p000 p<0,00 (p<0,001 p10 p=1,0 (p=1,00 p=0,77 p077 77 (p=0,77 (p=0,028 p096 p=0,96 96 (p=0,969 n= (n=1 p=0, p0 (p=0, p<0,0 (p<0,00 p1 p=1, (p=1,0 p=0,7 p07 7 (p=0,7 p09 p=0,9 9 (p=0,96 (n= p=0 (p=0 p<0, (p<0,0 p=1 (p=1, (p=0,9 (n p= (p= p<0 (p<0, (p=1 (p p< (p<0 (p<
Abstract To assess the effect of cleaning protocols on dentin contaminated with blood in reparative endodontic materials, bovine root samples were divided: no contamination (N); contamination (P); contamination and cleaning with saline (S), 2.5% NaOCl+saline (Na) or 2.5% NaOCl+17% EDTA+saline (NaE) and filled with: mineral trioxide aggregate (MTA), calcium-aluminate-cement (C), or C+collagen (Ccol) (n=13). The samples were evaluated for porosity, chemical composition, and bond strength. MTA porosity was lower than C (p=0.02) and higher than Ccol (p<0.001). P and NaE were similar (p=1.00), but higher than the other groups (p<0.001). MTA bond strength was similar to Ccol (p=0.777) and lower than C (p=0.028). P presented lower bond strength than the N (p<0.001); S and Na were similar to each other (p=0.969), but higher than P and lower than N (p<0.001). It was observed a predominance of mixed and cohesive failures. None of the samples showed Ca/P ratio values similar to human hydroxyapatite. This study showed that contamination with blood increased the materials porosity, but dentin cleaning with 2.5% NaOCl reduced this effect, and the collagen additive reduced the material porosity. Furthermore, blood contamination reduced the materials bond strength, and cleaning with saline or 2.5% NaOCl diminished this effect. divided (N) (P) S, , (S) 25 2 5 2.5 NaOClsaline (Na NaOCl17 17 NaOCl+17 EDTAsaline EDTA (NaE MTA, (MTA) calciumaluminatecement calcium aluminate cement C, (C) Ccollagen (Ccol n=13. n13 n n=13 . 13 (n=13) composition p=0.02 p002 p 0 02 (p=0.02 p<0.001. p0001 p<0.001 001 (p<0.001) p=1.00, p100 p=1.00 1 00 (p=1.00) p=0.777 p0777 777 (p=0.777 p=0.028. p0028 p=0.028 028 (p=0.028) p=0.969, p0969 p=0.969 969 (p=0.969) failures CaP Ca hydroxyapatite Furthermore (N (P (S 2. NaOCl1 NaOCl+1 (MTA (C n1 n=1 (n=13 p=0.0 p00 (p=0.0 p000 p<0.00 (p<0.001 p10 p=1.0 (p=1.00 p=0.77 p077 77 (p=0.77 (p=0.028 p096 p=0.96 96 (p=0.969 NaOCl+ n= (n=1 p=0. p0 (p=0. p<0.0 (p<0.00 p1 p=1. (p=1.0 p=0.7 p07 7 (p=0.7 p09 p=0.9 9 (p=0.96 (n= p=0 (p=0 p<0. (p<0.0 p=1 (p=1. (p=0.9 (n p= (p= p<0 (p<0. (p=1 (p p< (p<0 (p<
10.
Pioneer access of the foam nest bacterial community of Leptodactylidae frogs and its biotechnological potential
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Castro, L. G. Z.
; Sousa, M. R.
; Pereira, L. É. C.
; Martins, D. V.
; Oliveira, F. A. S.
; Bezerra, S. G. S.
; Melo, V. M. M.
; Hissa, D. C.
.
Resumo Muitos anfíbios anuros depositam seus ovos em ninhos de espuma, bioestruturas que ajudam a proteger os ovos e girinos de predadores. Atualmente, não existem relatos de identificação e descrição do papel da microbiota cultivável nos ninhos de sapos da família Leptodactylidae, como Physalaemus cuvieri, Leptodactylus vastus e Adenomera hylaedactyla. Este estudo teve como objetivo isolar e identificar as bactérias cultiváveis presentes nos ninhos dessas três espécies de anuros, além de prospectar enzimas produzidas por essa microbiota. Amostras de ninhos de espuma e amostras ambientais foram diluídas, e a contagem de células viáveis foi determinada. Os morfotipos bacterianos das amostras de ninhos de espuma foram isolados por meio da técnica de spread plate. O DNA dos isolados foram extraídos, seguidos pela amplificação do gene rRNA 16S e sequenciamento Sanger. Para avaliar o potencial enzimático, os isolados foram cultivados em meio ATGE suplementado com amido (0,1% p/v), gelatina (3% p/v) e leite desnatado (1% p/v), para verificar a atividade de amilase e proteases. Um total de 183 morfotipos bacterianos foi isolado, abrangendo 33 gêneros bacterianos. O filo Proteobacteria foi o mais abundante em todos os três ninhos (79%). Os gêneros Pseudomonas e Aeromonas foram os taxons mais abundantes em P. cuvieri e L. vastus. Em A. hylaedactyla, foram Enterobacter e Bacillus. Em relação às atividades enzimáticas, 130 isolados exibiram atividade de protease e 45 isolados foram positivos para atividade de amilase. Nossos resultados fornecem informações inéditas sobre a microbiota bacteriana cultivável dos ninhos de espuma dos sapos da família Leptodactylidae, bem como seu potencial para biomoléculas de interesse biotecnológico. predadores Atualmente Leptodactylidae hylaedactyla diluídas determinada plate extraídos S Sanger enzimático 0,1% 01 0 1 (0,1 p/v, pv p/v , p v 3% 3 (3 1% (1 proteases 18 isolado 79%. 79 79% . (79%) P L A Bacillus enzimáticas 13 4 biotecnológico 0,1 (0, ( 7 (79% 0, (0 (79 (7
Abstract Many anuran amphibians deposit their eggs in foam nests, biostructures that help protect the eggs and tadpoles from predators. Currently, there are no other identification and description studies of the cultivable microbiota role in the nests of the Leptodactylid frogs such as Physalaemus cuvieri, Leptodactylus vastus and Adenomera hylaedactyla. This study aimed to isolate and identify the culturable bacteria from these three anuran species' nests, as well as to prospect enzymes produced by this microbiota. Foam nests samples and environmental samples were diluted and viable cell count was determined. Bacterial morphotypes from foam nest samples were isolated through spread plate technique. Isolates’ DNAs were extracted followed by rRNA 16S gene amplification and Sanger sequencing. To evaluate their enzymatic potential, the isolates were cultured in ATGE medium supplemented with starch (0.1% w/v), gelatin (3% w/v) and skimmed milk (1% w/v), to verify amylase and protease activity. A total of 183 bacterial morphotypes were isolated, comprising 33 bacterial genera. Proteobacteria phylum was the most abundant in all the three nests (79%). The genera Pseudomonas and Aeromonas were the most abundant taxon in P. cuvieri and L. vastus. In A. Hylaedactyla, were Enterobacter and Bacillus. Regarding enzymatic activities, 130 isolates displayed protease activity and 45 isolates were positive for amylase activity. Our results provide unprecedented information concerning culturable bacterial microbiota of the foam nests of the Leptodactylid frogs, as well as their potential for biomolecules of biotechnological interest. predators Currently hylaedactyla species determined technique Isolates S sequencing 0.1% 01 0 1 (0.1 w/v, wv w/v , w v 3% 3 (3 1% (1 18 79%. 79 79% . (79%) P L Hylaedactyla Bacillus activities 13 4 interest 0.1 (0. ( 7 (79% 0. (0 (79 (7
11.
Biological and chemical characterization in relation to the yield of radish (Raphanus sativus L.) nourished with humus from plant residues Raphanus L. L
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Cruz Nieto, D. D.
; Muguruza Crispin, N. E.
; Caira Mamani, C. M
; Castro Bartolomé, H. J.
; Inga Sotelo, M. A.
; Alberto, M. N. M.
; Valderrama Romero, A. S.
; Vargas Godoy, V. C.
; More López, J. M.
; Chanducas Tantaleán, H. E.
; Menacho Javier, N. E.
; Lara Castillo, M. A.
; Flores Casanova, W.
; Benavente Ramírez, E. R.
; Apolín Montes, L. A.
; Diestra Salinas, F.
.
Resumo O aumento dos preços dos fertilizantes, energia e outros materiais necessários à indústria desencadeou uma crise econômica global. Motivo que foi investigado sobre as características biológicas e químicas em relação ao rendimento de rabanete nutrido com húmus de resíduo vegetal. O objetivo foi determinar a dose adequada de húmus para obtenção de maior rendimento e sua relação com as características químicas e biológicas do rabanete. Baseia-se em metodologia aplicada com abordagem experimental. Para tanto, utilizou-se o modelo de Delineamento em Blocos Completamente Aleatórios, que consistiu de 3 blocos e 5 tratamentos, que foram T1 com 0, T2 com 4, T3 com 6, T4 com 8 e T5 com 10 t/ha de húmus, e aplicaram 15 dias após a semeadura. As características físicas do rabanete foram avaliadas e processadas por meio de análise de variância e Duncan. Também foram analisadas a concentração de elementos nas folhas e a densidade estomática. Foi determinado que o T5 se destacou no comprimento total da planta com 28,95 cm, peso da planta com 76,87 g, diâmetro equatorial com 4.404 cm e produtividade comercial com 20.296 t/ha. Consumo de nitrogênio em relação à produtividade com 247,44 kg/ha. Densidade estomática 459 estômatos/mm2 e rentabilidade com 150% e concentração de nutrientes nas folhas destacadas T4 com N, K, Ca, Mg, Mo e Zn. Conclui que o T5 se destacou com 20.296 t/ha, que diferiu em 26,04% em relação ao controle (T1) com 15.011 t/ha. Portanto, esta dose adicionou nutrientes ao solo que melhoraram a disponibilidade para absorção pelas plantas e isso influenciou na concentração de nutrientes nas folhas como N, P e Fe e na densidade estomática com 459 estômatos/mm2, que teve resposta em bom desenvolvimento, fortalecendo contra o estresse ambiental e, portanto, maior desempenho. fertilizantes global vegetal Baseiase Baseia experimental tanto utilizouse utilizou Aleatórios tratamentos T 0 4 6 1 tha t ha semeadura Duncan 2895 28 95 28,9 7687 76 87 76,8 g 4404 404 4.40 20296 20 296 20.29 24744 247 44 247,4 kgha kg kg/ha 45 estômatosmm2 estômatosmm estômatos mm2 mm estômatos/mm 150 N K Ca Mg Zn 2604 26 04 26,04 (T1 15011 011 15.01 Portanto desenvolvimento portanto desempenho 289 2 9 28, 768 7 76, 440 40 4.4 2029 29 20.2 2474 24 247, 260 26,0 (T 1501 01 15.0 4. 202 20. 26, 15.
Abstract The increase in prices of fertilizers, energy and other materials necessary for the industry triggered a global economic crisis. Reason that was investigated on the biological and chemical characteristics in relation to the yield of radish nourished with humus from plant residue. The objective was to determine the appropriate dose of humus to obtain greater yield and its relationship with the chemical and biological characteristics of the radish. It is based on applied methodology with an experimental approach; Therefore, the Completely Random Block Design model was used, which consisted of 3 blocks and 5 treatments that were T1 with 0, T2 with 4, T3 with 6, T4 with 8 and T5 with 10 t/ha of humus and They applied 15 days after sowing. The physical characteristics of the radish were evaluated and processed using analysis of variance and Duncan. Concentration of elements in leaves and stomatal density were also analyzed. It was determined that T5 stood out in total plant length with 28.95 cm, plant weight with 76.87 g, equatorial diameter with 4,404 cm and commercial yield with 20,296 t/ha. Nitrogen consumption in relation to yield with 247.44 kg/ha. Stomatal density 459 stomata/mm2 and profitability with 150% and nutrient concentration in leaves highlighted T4 with N, K, Ca, Mg, Mo and Zn. It concludes that T5 stood out with 20,296 t/ha, which differed by 26.04% in relation to the control (T1) with 15,011 t/ha. Therefore, this dose added nutrients to the soil that improved the availability for plant absorption and this influenced the concentration of nutrients in leaves such as N, P and Fe and stomatal density with 459 stomata/mm2, which had a response in good development, strengthening against environmental stress and therefore greater performance. fertilizers crisis residue approach Therefore used T 0 4 6 1 tha t ha sowing Duncan analyzed 2895 28 95 28.9 7687 76 87 76.8 g 4404 404 4,40 20296 20 296 20,29 24744 247 44 247.4 kgha kg kg/ha 45 stomatamm2 stomatamm stomata mm2 mm stomata/mm 150 N K Ca Mg Zn 2604 26 04 26.04 (T1 15011 011 15,01 development performance 289 2 9 28. 768 7 76. 440 40 4,4 2029 29 20,2 2474 24 247. 260 26.0 (T 1501 01 15,0 202 20, 26. 15,
12.
First report on the characterization of the gut microbiota in Heliconius erato phyllis caterpillars (Lepidoptera-Nymphalidae) from southern Brazil LepidopteraNymphalidae Lepidoptera Nymphalidae (Lepidoptera-Nymphalidae
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13.
Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer Longterm Long term VEIL (VEIL
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Tobias-Machado, Marcos
; Ornellas, Antonio A.
; Hidaka, Alexandre K.
; Medina, Luis G.
; Mattos, Pablo A. L.
; Besio, Ruben S.
; Abreu, Diego
; Castro, Pedro R.
; Nishimoto, Ricardo H.
; Astigueta, Juan
; Dourado, Aurus
; Machado, Roberto D.
; Magnabosco, Wesley J.
; Corona-Montes, Victor
; Villoldo, Gustavo M.
; Zampolli, Hamilton C.
; Taha, Anis
; Auad, Pericles R.
; Faria, Eliney F.
; Arantes, Paulo B. O.
; Tavares, Alessandro
; Nascimento, Francisco S. M. S.
; Brazão Jr., Eder S.
; Rocha, Maurício M.
; Costa, Walter H.
; Panico, Vinicius
; Reis, Leonardo O.
; Almeida-Carrera, Roberto J.
; Silva, Rafael C.
; Zequi, Stênio C.
; Calixto, José R. R.
; Sotelo, Rene
.
ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer. Objective VEIL. . (VEIL) Methods 2 CoalitionLatin Coalition Latin PeCLA PeC LA (PeC-LA technique intermediate/highrisk intermediatehighrisk intermediate/high risk intermediate high nonfixed non fixed diameter range Results 200 2020 5 4568 45 68 (45-68 old 9 60120. 60120 60 120 (60-120) 1 616. 616 6 16 (6-16) 157 15 7 15.7% 19 1.9 86 8 8. 48 4.8 respectively 267 26 26.7 nonpalpable 28 2.8 10y y 742 74 74.2 848 84 84.8% pN0 pN pN1 100 100% 824 82 82.4% 727 72 72.7 91 9.1% Conclusion morbidity noninvasive invasive biopsy nonbulky bulky (VEIL highrisk intermediatehigh 20 202 456 (45-6 6012 12 (60-120 61 (6-16 15.7 1. 4. 26. 2. 74. 84.8 82.4 72. 9.1 (45- 601 (60-12 (6-1 15. 84. 82. 9. (45 (60-1 (6- (4 (60- (6 ( (60
14.
Synthesis and Anticancer Activity of Homodimeric Morita-Baylis-Hillman Adducts Based on 3-Hydroxyindolin-2-one Core MoritaBaylisHillman Morita Baylis Hillman 3Hydroxyindolin2one Hydroxyindolinone 3 Hydroxyindolin 2 one
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Coelho, Maísa C.
; Castro, Aleff
; Olegário, Tayná R.
; Cristiano, Rodrigo
; Vaz, Boniek G.
; Santos, Gabriel F. dos
; Machado, Lucas S.
; Militão, Gardênia C. G.
; Silva, Paulo B. N. da
; Vasconcellos, Mário L. A. A.
; Lima-Junior, Claudio G.
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
Cancer treatment represents one of the main scientific study targets today, mainly due to the pronounced side effects arising from chemotherapy. This study reports the synthesis, characterization, and anticancer activity of ten compounds from the Morita-Baylis-Hillman reaction. Ethylene glycol diacrylate was used as a double Michael acceptor in reactions with isatin derivatives to give homodimers of 3-hydroxyindolin-2-one core, recognized in the literature for its extensive pharmacological profile. The use of 1,4-diazabicyclo[2,2,2]octane (DABCO) as a catalyst and room temperature were the optimal conditions for the study reaction. The isolated yields were up to 63%, with most reaction times inferior to 24 h, some as fast as 15 min. The anticancer potential of the synthesized dimers was evaluated in vitro against three cancer strains, resulting in average inhibitory concentrations up to 0.72 µM. It was also found that the best performing homodimers are more active than their monomeric counterparts. Considering the promising selectivity indices observed, the preliminary results obtained here act as a basis for broader tests regarding the effectiveness of homodimeric adducts against cancer cells. today chemotherapy synthesis characterization MoritaBaylisHillman Morita Baylis Hillman 3hydroxyindolin2one hydroxyindolinone 3 hydroxyindolin 2 core profile 1,4diazabicyclo2,2,2octane 14diazabicyclo222octane diazabicyclooctane 1,4 diazabicyclo 2,2,2 octane 1 4 DABCO (DABCO 63 63% h min strains 072 0 72 0.7 µM counterparts observed cells 4diazabicyclo2 2octane 14 1, 222 2,2, 6 07 7 0. 4diazabicyclo 22 2,2 2,
15.
Ursolic Acid Derivatives Down Regulate Inflammatory Mediators
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Scherrer, Elaine C.
; Valadares, Ydia M.
; Alves, Caio C. S.
; Carli, Alessandra P.
; Fernandes, Bárbara G. R.
; Carvalho, Paloma E.
; Ramos, Karla A.
; Salvador, Maiara R.
; Silva, Jeferson G. da
; Silva, Fernando S.
; Denadai, Ângelo M. L.
; Castro, Sandra B. R.
.
Journal of the Brazilian Chemical Society
- Métricas do periódico
Ursolic acid (UA) is being investigated due to its anti-inflammatory potential, and structural modifications can enhance its biological activities. The aim of this study was to evaluate the immunomodulatory effect of the ursolic acid derivatives (UAD) in macrophages and in the carrageenan-induced paw edema model. RAW264.7 cells were cultured in the presence or absence of UA or UAD (1-18). Nitric oxide (NO), nuclear factor kappa B (NF-κB), tumor necrosis factor (TNF), and cellular viability were measured. 30 min before the carrageenan-induced paw edema, the UAD1 and UAD2 (200 mg kg-1) were administered intraperitoneally. The results showed that UAD2-4, UAD7, UAD9-11 had half maximal inhibitory concentration (IC50) greater than 90 µM and were able to reduce NO, NF-κB and TNF production. Moreover, UAD1 and UAD2 reduced paw edema and IL-6 production. In conclusion, the results obtained demonstrated a variation in the response between the derivatives due to the chemical modifications, showing potential to reduce the inflammatory mediators, deserving further investigations. (UA antiinflammatory anti activities (UAD carrageenaninduced carrageenan induced model RAW2647 RAW RAW264 7 RAW264. 118. 118 1 18 . (1-18) NO , (NO) NFκB, NFκB NF κB (NF-κB) TNF, (TNF) measured 3 200 (20 kg1 kg kg-1 intraperitoneally UAD24, UAD24 4, 4 UAD2-4 UAD7 UAD911 UAD9 11 UAD9-1 IC50 IC (IC50 9 production Moreover IL6 IL 6 IL- conclusion mediators investigations RAW26 (1-18 (NO (NF-κB (TNF 20 (2 kg- UAD2- UAD91 UAD9- IC5 (IC5 RAW2 (1-1 2 ( (IC (1- (1
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