Abstract The study aimed to compare the adherence of Brazilian and Italian pediatric dentists to the biosafety measures and operative protocols recommended by the health authorities during COVID-19 pandemic and to classify the participants according to their risk of infection. An online questionnaire with 34 questions about sociodemographic and occupational data, dental practice organization, biological risk management, and clinical operative protocols was sent to Brazilian and Italian pediatric dentists using a convenience sampling strategy. Chi-square test and multivariate analysis (two-step cluster) were performed (α = 5%). Of 641 respondents (377 Brazilians and 264 Italians), most were female (94% and 70%, respectively), aged 20-39 years (63%), with over 10 years of professional experience (58% and 49%, respectively). Based on adherence to recommended biosafety measures, participants were classified as “safer” (n = 219) or “less safe” (n = 422). Adherence to recommended protocols by the majority of participants resulted in low contagion rates (Brazilians = 5%; Italians = 12.5%). Participants with extensive professional experience in the dental setting exhibited a greater tendency to implement multiple adaptations (three or more) in their practice. Most participants (Brazilians = 92%; Italians = 80.7%) adopted the recommended minimal intervention dentistry approaches, with the use of fissure sealants and the use of non-rotary instruments for caries removal the most frequently techniques used among Brazilians (36%) and Italians (66%), respectively. Two different profiles of pediatric dentists were identified based on the biosafety protocols adopted during the pandemic. In addition, changes were implemented in the dental care provided to children, with focus on the minimal intervention dentistry. COVID19 COVID 19 COVID-1 infection 3 data organization management strategy Chisquare Chi square twostep two step cluster α 5%. 5 5% . 5%) 64 377 (37 26 Italians, , Italians) 94% 94 (94 70 70% respectively, respectively respectively) 2039 20 39 20-3 63%, 63 63% (63%) 1 58% 58 (58 49 49% safer “safer n 219 less safe 422. 422 422) 12.5%. 125 12.5% 12 12.5%) three more 92% 92 80.7% 807 80 7 approaches nonrotary non rotary 36% 36 (36% 66%, 66 66% (66%) addition children COVID1 COVID- 6 37 (3 2 9 (9 203 20- (63% (5 4 21 42 12.5 80.7 8 (36 (66% ( (63 12. 80. (66 (6

RESUMO Taninos condensados são compostos oriundos do metabolismo secundário das plantas. Eles são de especial interesse devido à sua ampla gama de aplicações e usos tecnológicos. O objetivo do presente trabalho foi extrair, quantificar e caracterizar por espectroscopia de infravermelho com transformada de Fourier (FTIR) os taninos da casca de quatro espécies cultivadas na região Nordeste do Brazil: Acacia mangium, Azadirachta indica, Mimosa tenuiflora (‘jurema preta’) e Mimosa caesalpiniifolia (‘sabiá’ ou ‘sansão-do-campo). Amostras de 5 árvores de cada espécie foram coletadas, moídas e extraídas com água quente, determinandose os rendimentos de extração. A partir dos extratos foram determinados o teor de sólidos totais (TST), o índice de Stiasny (IS) e o teor de taninos condensados (TTC). Os espectros de FTIR foram adquiridos a partir de amostras de taninos imobilizados em pastilhas de brometo de potássio. Dentre as quatro espécies, M. tenuiflora teve o mais alto TTC igual a 23,4% e o extrato bruto mais puro com IS de 91,27%. Com base na caracterização por FTIR, os taninos das cascas de M. caesalpiniifolia, A. indica e A. mangium foram classificados como proantocianidinas e os da casca de M. tenuiflora como prodelfinidinas.

ABSTRACT Condensed tannins are chemical compounds derived from the secondary metabolism of plants. They are of particular interest due to their broad range of technological uses and applications. The objective of this study was to extract, quantify, and characterize by Fourier-transform infrared spectroscopy (FTIR) the bark tannins of four forest species grown in Northeast Brazil: Acacia mangium, Azadirachta indica, Mimosa tenuiflora, and Mimosa caesalpiniifolia. Bark samples were collected from 5 trees of each species grown in a five-year-old forest stand. Samples were collected, grounded, and submitted to extraction with hot water, then measured to determine total solids content (TSC), Stiasny number (SN), and condensed tannins content (CTC). FTIR spectra were acquired from tannin samples fixed in potassium bromide pellets. Among the four species, M. tenuiflora presented the highest CTC, equal to 23.4%, and the purest crude hot-water extract with SN of 91.27%. Based on FTIR characterization, tannins from M. caesalpiniifolia, A. indica, and A. mangium barks were classified as proanthocyanidins and those from M. tenuiflora bark as prodelphinidins.

Abstract In a previous study, we demonstrated that the incorporation of doxycycline hyclate (DOX) into resin-modified glass ionomer cement (RMGIC) inhibited important cariogenic microorganisms, without modifying its biological and mechanical characteristics. In this study, we keep focused on the effect of that experimental material as a potential therapy for arresting residual caries by analyzing other in vitro properties and conducting a pilot clinical trial assessing the in vivo effect of DOX-containing RMGIC on residual mutans streptococci after partial carious removal in primary molars. Specimens of the groups RMGIC (control); RMGIC + 1.5% DOX; RMGIC + 3% DOX; and RMGIC + 4.5% DOX were made to evaluate the effect of DOX incorporation on surface microhardness and fluoride release of RMGIC and against biofilm of Streptococcus mutans. Clinical intervention consisted of partial caries removal comparing RMGIC and RMGIC + 4.5% DOX as lining materials. After 3 months, clinical and microbiologic evaluations were performed. Data were submitted to ANOVA/Tukey or Wilcoxon/Mann-Whitney set as α=0.05. Fluoride release and surface microhardness was not influenced by the incorporation of DOX (p>0.05). There was a significant reduction of S. mutans biofilm over the material surface with the increase of DOX concentration. After clinical trial, the remaining dentin was hard and dry. Additionally, mutans streptococci were completely eliminated after 3 months of treatment with RMGIC + 4.5% DOX. The incorporation of DOX provided better antibiofilm effect, without jeopardizing fluoride release and surface microhardness of RMGIC. This combination also improved the in vivo shortterm microbiological effect of RMGIC after partial caries removal.

Abstract Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed using agar diffusion and anti-biofilm assays. Cytotoxic effects, compressive tensile strength, microhardness and fluoride (F) release were also evaluated. A randomized controlled trial was conducted on 36 children that received ART either with GIC or GIC with CHX. Saliva and biofilm were collected for mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p<0.05). Results: Incorporation of 1.25% and 2.5% CHX improved the antimicrobial/anti-biofilm activity of GIC, without affecting F release and mechanical characteristics, but 2.5% CHX was cytotoxic. Survival rate of restorations using GIC with 1.25% CHX was similar to GIC. A significant reduction of MS levels was observed for KM+CHX group in children saliva and biofilm 7 days after treatment. Conclusions: The incorporation of 1.25% CHX increased the in vitro antimicrobial activity, without changing chemical-mechanical properties of GIC and odontoblast-like cell viability. This combination improved the in vivo short-term microbiological effect without affecting clinical performance of ART restorations.

Objective This in vitro study assessed the anti-erosive effect of experimental mouthrinses containing TiF4 and NaF on dentin erosive loss. Material and Methods Bovine dentin specimens were randomly allocated into the groups (n=15): 1) SnCl2/NaF/AmF (Erosion Protection®/GABA, pH 4.5, positive control); 2) experimental solution with 0.0815% TiF4 (pH 2.5); 3) 0.105% NaF (pH 4.5); 4) 0.042% NaF+0.049% TiF4 (pH 4.4); 5) 0.063% NaF+0.036% TiF4 (pH 4.5); 6) no treatment (negative control). Each specimen was cyclically demineralized (Sprite Zero, pH 2.6, 4x90 s/day) and exposed to artificial saliva between the erosive challenges for 7 days. The treatment with the fluoride solutions was done 2x60 s/day, immediately after the first and the last erosive challenges of the day. Dentin erosive loss was measured by profilometry (μm). The data were analyzed using Kruskal Wallis/Dunn tests (p<0.05). Results Mouthrinses containing TiF4 or Sn/F were able to show some protective effect against dentin erosive loss compared to negative control. The best anti-erosive effect was found for experimental solution containing 0.0815% TiF4 (100% reduction in dentin loss), followed by 0.042% NaF+0.049% TiF4 (58.3%), SnCl2/NaF/AmF (52%) and 0.063% NaF+0.036% TiF4 (40%). NaF solution (13.3%) did not significantly differ from control. Conclusion The daily application of experimental mouthrinse containing TiF4 and NaF has the ability to reduce dentin erosion, as well as Erosion Protection® and TiF4 alone.

OBJETIVO: Envolver modelos atuais e comprovações científicas sobre a influência de comportamentos de saúde bucal dos pais na cárie dentária de suas crianças. FONTES: Artigos do MEDLINE publicados entre 1980 e junho de 2012. Foram analisados artigos de pesquisa originais tratando do comportamento dos pais quanto à saúde bucal. Um total de 218 citações foi analisado e 13 artigos foram incluídos na análise. Os estudos foram considerados elegíveis para análise se atendessem aos seguintes critérios de inclusão: (1) avaliassem uma possível associação entre cáries dentárias e o comportamento dos pais relacionado à saúde bucal; e (2) se a metodologia do estudo incluísse exame clínico bucal. Os principais termos de pesquisa foram "saúde bucal", "atitudes dos pais", "conhecimento dos pais" e "cáries dentárias". RESUMO DOS ACHADOS: Ao todo, 13 estudos experimentais contribuíram com dados para a síntese. Também foram considerados trabalhos, revisões e capítulos originais em livros didáticos. CONCLUSÃO: Os hábitos de saúde dental dos pais influenciam a saúde bucal de seus filhos. São necessários programas de educação em saúde bucal com ações preventivas para proporcionar não apenas saúde bucal adequada às crianças, mas também uma melhor qualidade de vida. Deve ser dada atenção especial a toda a família, com relação a seu estilo de vida e hábitos relacionados à saúde bucal.

OBJECTIVE: To review current models and scientific evidence on the influence of parents' oral health behaviors on their children's dental caries. SOURCES: MEDLINE articles published between 1980 and June, 2012. Original research articles on parents' oral health behavior were reviewed. A total of 218 citations were retrieved, and 13 articles were included in the analysis. The studies were eligible for review if they matched the following inclusion criteria: (1) they evaluated a possible association between dental caries and parents' oral-health-related behaviors, and (2) the study methodology included oral clinical examination. The main search terms were "oral health", "parental attitudes", "parental knowledge", and "dental caries". SUMMARY OF THE FINDINGS: 13 experimental studies contributed data to the synthesis. Original articles, reviews, and chapters in textbooks were also considered. CONCLUSIONS: Parents' dental health habits influence their children's oral health. Oral health education programs aimed at preventive actions are needed to provide children not only with adequate oral health, but better quality of life. Special attention should be given to the entire family, concerning their lifestyle and oral health habits.

O objetivo deste trabalho foi verificar a incidência de cárie dentária por meio dos índices CPO-D, CPO-S, ceo-d e ceo-s em indivíduos com síndrome de Down regularmente matriculados em um programa preventivo. Foram examinados 24 indivíduos com síndrome de Down de ambos os sexos, com idade entre um e 48 anos. A prevalência (situação experimental inicial) e a incidência (situação experimental final) de cárie dentária foram verificadas utilizando-se os índices CPO-D, CPO-S, ceo-d e ceo-s iniciais e finais dos participantes. Dos 24 indivíduos examinados, dez (42,0%) eram livres de cárie. A prevalência de cárie dentária mostrou valores de CPO-D= 2,33; CPO-S= 3,60; ceo-d= 1,75 e ceo-s= 2,80; enquanto a incidência de cárie apresentou valores de 2,33; 3,80; 1,10 e 1,90, respectivamente. Os indivíduos com síndrome de Down avaliados neste estudo apresentaram baixos índices de cárie e pequena incidência de novas lesões, enfatizando a importância da manutenção desses pacientes em programas preventivos.

The objective of this work was to verify the incidence of dental caries by means of the CPO-D, CPO-S, ceo-d and ceo-s indexes in patients with Down syndrome regularly enrolled in a preventive program. Twenty four Down syndrome patients of both sexes age range of one to 48 years were examined. The prevalence (initial experimental situation) and incidence (final experimental situation) of dental caries were verified using of the initial and final CPO-D, CPO-S, ceo-d and ceo-s indexes of the participants. From 24 individual examined, 10 (42.0%) were free of caries. The prevalence of dental caries showed values of CPO-D= 2.33; CPO-S= 3.60; ceo-d= 1.75 e ceo-s= 2.80; while the incidence of caries showed values of 2.33; 3.80; 1.10 e 1.90, respectively. Down syndrome individuals evaluated in this study presented low level of caries and small incidence of new lesions, emphasizing the importance of the maintenance of these patients at preventive programs.

O objetivo deste trabalho foi verificar a incidência de cárie dentária por meio dos índices CPO-D, CPO-S, ceo-d e ceo-s em indivíduos com síndrome de Down regularmente matriculados em um programa preventivo. Foram examinados 24 indivíduos com síndrome de Down de ambos os sexos, com idade entre um e 48 anos. A prevalência (situação experimental inicial) e a incidência (situação experimental final) de cárie dentária foram verificadas utilizando-se os índices CPO-D, CPO-S, ceo-d e ceo-s iniciais e finais dos participantes. Dos 24 indivíduos examinados, dez (42,0%) eram livres de cárie. A prevalência de cárie dentária mostrou valores de CPO-D= 2,33; CPO-S= 3,60; ceo-d= 1,75 e ceo-s= 2,80; enquanto a incidência de cárie apresentou valores de 2,33; 3,80; 1,10 e 1,90, respectivamente. Os indivíduos com síndrome de Down avaliados neste estudo apresentaram baixos índices de cárie e pequena incidência de novas lesões, enfatizando a importância da manutenção desses pacientes em programas preventivos.

The objective of this work was to verify the incidence of dental caries by means of the CPO-D, CPO-S, ceo-d and ceo-s indexes in patients with Down syndrome regularly enrolled in a preventive program. Twenty four Down syndrome patients of both sexes age range of one to 48 years were examined. The prevalence (initial experimental situation) and incidence (final experimental situation) of dental caries were verified using of the initial and final CPO-D, CPO-S, ceo-d and ceo-s indexes of the participants. From 24 individual examined, 10 (42.0%) were free of caries. The prevalence of dental caries showed values of CPO-D= 2.33; CPO-S= 3.60; ceo-d= 1.75 e ceo-s= 2.80; while the incidence of caries showed values of 2.33; 3.80; 1.10 e 1.90, respectively. Down syndrome individuals evaluated in this study presented low level of caries and small incidence of new lesions, emphasizing the importance of the maintenance of these patients at preventive programs.

O interesse pela qualidade de vida e saúde bucal em crianças e adolescentes vem aumentando, já que as desordens orais provavelmente apresentam efeito negativo na qualidade de vida das mesmas. Neste sentido, questionários que visam a avaliar o impacto da saúde bucal no bem-estar têm sido desenvolvidos e adaptados para este grupo específico. O objetivo deste trabalho é descrever a relação entre qualidade de vida e saúde bucal em crianças e adolescentes, focalizando aspectos conceituais e metodológicos. Os instrumentos desenvolvidos para avaliar a qualidade de vida e saúde bucal em crianças e adolescentes são: o Child Perceptions Questionnaire (CPQ), para crianças de 8-10 (CPQ8-10) e 11-14 anos (CPQ11-14); o Child-Oral Impacts on Daily Performances, para a idade de 11-12 anos; o Child Oral Health Impact Profile, para crianças de 8-14 anos, e o Early Childhood Oral Health Impact Scale para a faixa etária de 2-5 anos. Apesar dos progressos nessa área, ainda existem muitas questões a serem debatidas e respondidas, como a necessidade de questionários específicos para diferentes idades, a participação dos responsáveis como respondentes secundários e a avaliação do impacto da saúde bucal da criança e do adolescente na família.

There has been increasing interest in the oral health-related quality of life of children and adolescents, since pediatric oral disorders are likely to have a negative effect on children's quality of life. So the questionnaires that aim to assess the impact of oral health on child' and adolescent' quality of life has been developed or adapted to this specific group. The aim of this study was to describe the relationship between the quality of life and oral health in children and adolescents, focalizing conceptual and methodological aspects. The instruments developed to assess oral health-related quality of life in children and adolescents are: the Child Perceptions Questionnaire (CPQ), for children aged 8-10 (CPQ8-10) and 11-14 (CPQ11-14), the Child-Oral Impacts on Daily Performances, for children aged 11-12, the Child Oral Health Impact Profile, for children aged 8-14, and the Early Childhood Oral Health Impact Scale for children aged 2-5. Although progress was observed in this area, there are still many issues to be discussed and answered, as the need for specific questionnaires for different ages, the participation of respondents as secondary and evaluation of the impact of child' and adolescent' oral health in the family.

O interesse pela qualidade de vida e saúde bucal em crianças e adolescentes vem aumentando, já que as desordens orais provavelmente apresentam efeito negativo na qualidade de vida das mesmas. Neste sentido, questionários que visam a avaliar o impacto da saúde bucal no bem-estar têm sido desenvolvidos e adaptados para este grupo específico. O objetivo deste trabalho é descrever a relação entre qualidade de vida e saúde bucal em crianças e adolescentes, focalizando aspectos conceituais e metodológicos. Os instrumentos desenvolvidos para avaliar a qualidade de vida e saúde bucal em crianças e adolescentes são: o Child Perceptions Questionnaire (CPQ), para crianças de 8-10 (CPQ8-10) e 11-14 anos (CPQ11-14); o Child-Oral Impacts on Daily Performances, para a idade de 11-12 anos; o Child Oral Health Impact Profile, para crianças de 8-14 anos, e o Early Childhood Oral Health Impact Scale para a faixa etária de 2-5 anos. Apesar dos progressos nessa área, ainda existem muitas questões a serem debatidas e respondidas, como a necessidade de questionários específicos para diferentes idades, a participação dos responsáveis como respondentes secundários e a avaliação do impacto da saúde bucal da criança e do adolescente na família.

There has been increasing interest in the oral health-related quality of life of children and adolescents, since pediatric oral disorders are likely to have a negative effect on children's quality of life. So the questionnaires that aim to assess the impact of oral health on child' and adolescent' quality of life has been developed or adapted to this specific group. The aim of this study was to describe the relationship between the quality of life and oral health in children and adolescents, focalizing conceptual and methodological aspects. The instruments developed to assess oral health-related quality of life in children and adolescents are: the Child Perceptions Questionnaire (CPQ), for children aged 8-10 (CPQ8-10) and 11-14 (CPQ11-14), the Child-Oral Impacts on Daily Performances, for children aged 11-12, the Child Oral Health Impact Profile, for children aged 8-14, and the Early Childhood Oral Health Impact Scale for children aged 2-5. Although progress was observed in this area, there are still many issues to be discussed and answered, as the need for specific questionnaires for different ages, the participation of respondents as secondary and evaluation of the impact of child' and adolescent' oral health in the family.

La Incontinentia Pigmenti es una condición rara, que afecta órganos y tejidos de origen ectodérmico y mesodérmico, asociado al cromosoma X dominante. Se manifiesta en el nacimiento o más tarde, con lesiones eritematosas en la piel. Puede afectar también pelo, uñas, ojos, sistema nervioso central y dientes. Las manifestaciones dentarias presentes se parecen con aquellas encontradas en la displasia ectodérmica y en la sífilis congénita: hipodoncia, microdoncia, retraso de la dentición, malformaciones y dientes impactados. Las alteraciones dentarias que se presentan en tal caso son típicas y comprenden agenesias, dientes conoides y disturbios de la erupción.

Incontinentia Pigmenti is one of the rare conditions that affect organs and tissues of ectodermal and mesodermal origin, and is inherited as an X-linked dominant trait. It may be noticed at birth or soon after, by means of skin erythematous lesions. It may also affect hair, nails, eyes, central nervous system and teeth. Dental manifestations are similar to those observed on ectodermal dysplasia and congenital syphilis: hypodontia, microdontia, delayed tooth eruption, malformations and impacted teeth. The dental alterations presented on this case report are typical and include tooth agenesis, peg-shaped teeth and eruption disturbances. A clinical case of a girl is presented in that observes the cutaneous hiperpigmentation of the superior members and the back; and the clinical absence of several temporary teeth.

Incontinentia Pigmenti é uma condição rara, que afeta órgãos e os tece da origem ectodérmico e mesodermico, associado ao cromosoma dominante X. É pronunciado mais atrasado no nascimento ou, com os ferimentos erytematosus na pele. Pode também afetar o cabelo, pregos, olhos, sistema nervoso central e dentes. Os manifestação dentais atuais olham como com aqueles encontrados dentro ectodérmica e sífilis da displasia congênita: hipodontia, microdontia, atrasa da má formação dentarias e dentes impactados. As alterações dentais que aparecem em tal caso são típicas e agenesias de inclui dentes dos conoides e distúrbios da erupção. Um exemplo clínico de uma menina aparece em quem eu sou observado o hiperpigmentassem cutâneos dos membros superiores e da parte traseira; hipotricosis e a ausência clínica de diversos dentes decíduos.