Abstract Objectives This study aims to evaluate the characteristics and treatment response of patients with pityriasis lichenoides seen in the last 43 years in a pediatric dermatology service. Methods This was a retrospective, analytical, longitudinal study of patients under 15 years of age. The medical records were reviewed and data were presented as frequencies, means and variances. Student's t-test, Mann-Whitney test, Fisher's exact test, Pearson/Yates chi-square test and multivariate logistic regression model were used, with p < 0.05 considered. Results 41 patients were included, 32 (78.0%) with pityriasis lichenoides chronica (PLC), five (12.2%) with pityriasis lichenoides et varioliformis acuta (PLEVA) and four (9.8%) with clinical PLC without biopsy. The age range of school children and adolescents was 19 (46.3%) and 13 (31.7%) respectively and 27 (65.8%) were male. Two peaks of the highest frequency were observed between 2004 and 2006 (10 patients - 24.4%) and another between 2019 and 2021 (6 patients - 14.7%). There was remission in 71.9% (n = 23), with 56.6% (n = 17) of those who used antibiotic therapy and 80% (n = 4) of those who had phototherapy. The chance of remission was 13 times greater in patients with disease onset after 5 years of age. Conclusions The clinical form most commonly found was PLC mainly in school children and adolescents. The frequency peaks coincided with infectious outbreaks. The remission rate was satisfactory with antibiotic therapy, but higher with phototherapy. Remission was greater in patients with disease onset after 5 years of age. 4 service retrospective analytical 1 frequencies variances Students Student s ttest, ttest t t-test MannWhitney Mann Whitney Fishers Fisher PearsonYates Pearson Yates chisquare chi square 005 0 05 0.0 considered included 3 78.0% 780 78 (78.0% PLC, , (PLC) 12.2% 122 12 2 (12.2% PLEVA (PLEVA 9.8% 98 9 8 (9.8% biopsy 46.3% 463 46 (46.3% 31.7% 317 31 7 (31.7% 65.8% 658 65 (65.8% male 200 10 (1 24.4% 244 24 201 202 6 ( 14.7%. 147 14.7% . 14 14.7%) 719 71 71.9 n 23, 23 23) 566 56 56.6 17 80 phototherapy outbreaks 00 0. 78.0 (78.0 (PLC 12.2 (12.2 9.8 (9.8 46.3 (46.3 31.7 (31.7 65.8 (65.8 20 24.4 14.7 71. 56. 78. (78. 12. (12. 9. (9. 46. (46. 31. (31. 65. (65. 24. 14. (78 (12 (9 (46 (31 (65 (7 (4 (3

Abstract Objective: Perfume (Parfum) or fragrance is a natural or synthetic cosmetic ingredient added to emit a pleasant aroma or to improve the odor of a cosmetic formula. It is a mixture of substances, not revealed by the manufacturer, which may contain ingredients with allergenic potential, endocrine disruptors, and other possible harmful effects on human health. This study aims to analyze children’s cosmetics labels to assess the presence of Perfume. Methods: The researchers randomly visited points of sale in Curitiba, the capital of a southern Brazilian state; in order to catalog the largest possible number of children’s cosmetics items. Results: 398 children’s cosmetics were analyzed and found Parfum on 295 (74.1 %) of the labels, including 90.4 and 79,1 % of the shampoos and wet wipes, respectively. Conclusion: Exposure of children’s skin to fragrances can lead to local side effects such as allergies, but also to systemic effects, and the lack of knowledge of the general population and health professionals about its possible deleterious effects emphasizes the importance of changes in the regulation of cosmetics aiming to reduce the use of this ingredient. Objective (Parfum formula substances manufacturer potential disruptors childrens children s Methods Curitiba state items Results 39 29 74.1 741 74 1 (74. 904 90 4 90. 791 79 79, wipes respectively Conclusion allergies 3 2 74. 7 (74 9 (7 (

Abstract Introduction: The association of hepatitis delta virus (HDV) infection with positive autoantibodies and autoimmune features has been known for decades. However, to date, very few cases of clinical autoimmune hepatitis (AIH) have been reported in association with HDV infection, most of them being in the context of treatment with peginterferon. Case Report: This case refers to a 46-year-old woman born in Guinea-Bissau who moved to Portugal in 2018 to investigate complaints of diffuse abdominal discomfort and nausea. Her initial work-up, including laboratory and liver histology, was consistent with type 1 AIH. She had HBe antigen-negative chronic hepatitis B virus infection with negative DNA and also a positive total anti-HDV antibody, with negative IgM and undetectable RNA. Therefore, after initiating prophylactic tenofovir difumarate, she was started on prednisolone followed by azathioprine, which was later stopped due to presumed hepatotoxicity. Repeated histology showed signs of viral superinfection, and she was treated with acyclovir due to a positive herpes simplex IgM, with HDV RNA remaining negative. A third flare in transaminases prompted the introduction of mycophenolate mofetil (MMF) after a thorough exclusion of additional causes of liver disease. About 6 months later, during another bout of hepatitis, HDV RNA was finally positive and classified as genotype 5. MMF was stopped, and, considering a contraindication to interferon, the patient was offered therapy with bulevirtide, which she refused for personal reasons as she is currently living in her home country. Discussion: This is a challenging case of autoimmune or “autoimmune-like” hepatitis, probably induced by chronic HDV infection. High suspicion of HDV was essential because, had the case been interpreted as refractory AIH, with escalation of immunosuppression, a more severe course of the viral infection might have ensued. Recently, HDV suppression with bulevirtide was shown to reverse autoimmune liver disease. We hypothesize that the same could have happened to our patient, had she accepted this treatment.

Resumo Introdução: A associação da infeção pelo vírus da hepatite delta (VHD) com a presença de autoanticorpos e outros aspetos de autoimunidade é conhecida desde há várias décadas. Contudo, até à data, muito poucos casos de hepatite autoimune (HAI) clínica foram reportados em relação com a infeção VHD, sendo a maioria destes no contexto de terapêutica com interferão peguilado. Caso clínico: O caso refere-se a uma mulher de 46 anos natural da Guiné-Bissau, que se mudou para Portugal em 2018 para investigação de queixas de desconforto abdominal difuso e náuseas. A avaliação laboratorial inicial e a histologia hepática foram compatíveis com HAI tipo 1. A doente apresentava também infeção crónica a VHB (vírus da hepatite B) antigénio HBe negativa, com DNA negativo, e anti-VHD (vírus da hepatite delta) total positivo, com IgM negativo e RNA indetetável. Assim, após início de tenofovir difumarato profilático, foi iniciada terapêutica com prednisolona seguida de azatioprina, que posteriormente se interrompeu por presumível hepatotoxicidade. Uma segunda biópsia mostrou aspetos de superinfeção viral e como tal a doente foi tratada com aciclovir, tendo em conta IgM positivo para Herpes Simplex, mantendo-se o RNA VHD negativo. Um terceiro flare de transaminases motivou o início de micofenolato de mofetil, após extensa investigação e exclusão de outras causas de doença hepática. Cerca de 6 meses mais tarde, durante novo episódio de hepatite, o RNA VHD revelou-se finalmente positivo e este foi classificado como genotipo 5. O MMF foi suspenso e, considerando a contra-indicação para interferão, foi proposto à doente tratamento com bulevirtide, que esta recusou, alegando motivos pessoais, visto estar atualmente a residir no seu país de origem. Discussão: Este é um caso desafiante de hepatite autoimune, ou autoimune-like, provavelmente induzida pela infeção crónica pelo VHD. Um elevado índice de suspeição para VHD foi essencial porque, se o caso tivesse sido interpretado como HAI refratária, com incremento de imunossupressão, poderia ter-se verificado um agravamento da hepatite viral. Recentemente, foi reportado que a supressão do VHD pelo bulevirtide pode reverter a doença hepática autoimune. Questionamo-nos se o mesmo poderia ter sucedido com a nossa doente, caso esta tivesse aceite este tratamento.

Abstract Introduction: The discussion on palliative care in high-tech environments and interventional practice represents one of the various aspects to be considered in the challenge of providing a continuum of wellness in living and dying for the elderly. Objective: To map and identify the existing works in the literature on nursing actions that focus on palliative care for elderly patients in surgical hospitalization settings. Materials and methods: This scoping review was conducted on eight databases, following the Joanna Briggs Institute and the Preferred Reporting Items for Systematic Review-Scoping Review guidelines. The following descriptors were used: nursing care; palliative care; elderly; surgery. The inclusion criteria were the following: primary research, systematic reviews, meta-analyses, and clinical trials. The search was performed in three stages: database listing / pilot test; broad search/ application of the "PCC" strategy (population, concept, context); and full reading of the content. A total of 509 productions were retrieved and then managed using the Rayyan® software, of which 13 studies were selected. The protocol was registered in the Open Science Framework. Results: The total population consisted of 10,417 people aged from 60 to 109. The most frequent interventions included the physical dimension, for symptomatic control; the cultural dimension, in which the dilemmas present in an environment with a high expectation for recovery and rigid interventionist protocols were highlighted, as well as the communication dimension, which is a basic tool of palliative care. Conclusions: Elderly people with serious conditions can benefit from palliative care in the perioperative setting. However, there is a need for training nurses in pain management, empowerment to participate in ethical decisions, and training for better communication. It should be noted that evidence on interventions to improve palliative care is still limited by methodological flaws, so rigorous evaluations are needed to measure significant results for patients and care.

Resumen Introducción: La discusión sobre los cuidados paliativos en ambientes de alta tecnología y prácticas intervencionistas es uno de los muchos aspectos a considerar en el desafío de proporcionar un continuum de vivir y morir bien a los ancianos. Objetivo: Mapear e identificar la literatura existente sobre las acciones de enfermería dirigidas a los cuidados paliativos de los pacientes ancianos en el contexto de la hospitalización quirúrgica. Materiales y método: Se realizó una revisión exploratoria en ocho bases de datos, siguiendo las directrices del Instituto Joanna Briggs y los Preferred Reporting Items for Systematic Review-Scoping Review. Se utilizaron los siguientes descriptores: cuidados de enfermería; cuidados paliativos; ancianos; cirugía. Se seleccionaron como criterios de inclusión investigaciones primarias, revisiones sistemáticas, metaanálisis y ensayos clínicos. La búsqueda se realizó en tres etapas: listado base/ aplicación de prueba piloto; búsqueda amplia/aplicación de la estrategia "PCC" (population, concept, context); lectura completa de los materiales. Se devolvieron 509 producciones, gestionadas mediante el software Rayyan®, y se seleccionaron 13 estudios. El protocolo se registró en el Open Science Framework. Resultados: La población única total fue de 10 417 personas de entre 60 y 109 años. Las intervenciones más frecuentes se refieren a la dimensión física, de control sintomático; la dimensión cultural, en la que destacan los dilemas presentes en un entorno con altas expectativas de curación y rígidos protocolos intervencionistas, así como la dimensión comunicativa, herramienta básica de los cuidados paliativos. Conclusiones: Los ancianos con enfermedades graves pueden beneficiarse de los cuidados paliativos en el entorno perioperatorio. Sin embargo, es necesario formar al equipo de enfermería en el control del dolor, capacitarlo para participar en decisiones éticas y capacitarlo para una mejor comunicación. Cabe señalar que las pruebas sobre las intervenciones para mejorar los cuidados paliativos siguen estando limitadas por defectos metodológicos, por lo que se necesitan evaluaciones rigurosas para medir resultados significativos para los pacientes y la atención.

Resumo Introdução: A discussão sobre cuidados paliativos em ambientes de alta tecnologia e prática intervencionista representa um dos muitos aspectos a serem considerados no desafio de proporcionar um continuum de viver e morrer bem para a pessoa idosa. Objetivo: Mapear e identificar na literatura as produções existentes sobre as ações de enfermagem voltadas ao cuidado paliativo ao paciente idoso no contexto de hospitalização cirúrgica. Materiais e método: Revisão de escopo, realizada em oito bases de dados, seguindo as diretrizes do Joanna Briggs Institute e do Preferred Reporting Items for Systematic Review-Scoping Review. Foram utilizados os seguintes descritores: cuidados de enfermagem; cuidados paliativos; idosos; cirurgia. Como critério de inclusão, foram selecionadas pesquisas primárias, revisões sistemáticas, metanálises e ensaios clínicos. A busca foi realizada em três etapas: arrolamento das bases/aplicação de teste-piloto; busca ampla/aplicação da estratégia "PCC" (population, concept, context); leitura completa dos materiais. Foram retornadas 509 produções, gerenciadas no software Rayyan®, das quais 13 estudos foram selecionados. O protocolo foi registrado no Open Science Framework. Resultados: A população total única foi de 10 417 pessoas, entre 60 e 109 anos. As intervenções mais frequentes dizem respeito à dimensão física, de controle sintomático; à dimensão cultural, na qual se destacam os dilemas presentes em ambiente com alta expectativa de cura e rígidos protocolos intervencionistas, bem como à dimensão da comunicação, ferramenta básica do cuidado paliativo. Conclusões: Pessoas idosas com doenças graves podem se beneficiar do cuidado paliativo no ambiente perioperatório. No entanto, há necessidade de aperfeiçoamento de enfermeiros no controle da dor, no empoderamento para a participação em decisões éticas e na capacitação para uma melhor comunicação. Ressalta-se que as evidências sobre intervenções para melhorar os cuidados paliativos ainda são limitadas por falhas metodológicas, portanto são necessárias avaliações rigorosas que meçam resultados significativos para os pacientes e para a assistência.

ABSTRACT: Plant extracts effects have been regarded and proven in researches under different applications in agriculture, including seed treatment. The plant extracts effectiveness is attributed to the presence of certain classes of compounds; therefore, studies that aim to identify and quantify these composites, which are present in plant extracts used in agriculture, are important, as well as the seed treatment. This research aimed to understand and describe through a systematic review, what were the main carried approaches, classes and identified compounds in studies with plant extracts to different applications in agriculture and the seed treatment. The period 2015 to 2020 was the one that exposed the higher research publication indexes, considering the theme under analysis, plant extracts in agriculture, seeking to identify or quantify the presence of metabolic composites, indicating a growing interest in this theme. Such studies pursued identifying and/or quantifying the compounds that are present in the plant extracts. The phenolic compounds constitute the priority class of metabolites to different functions, effects and applications in agriculture, mainly in seed treatment. The terpenes present a substantial potential as bioinsecticides to agriculture. Plant species that are rich in phenolic and terpenes compounds are a significant source of alternative control in the protection of the productive system. ABSTRACT treatment therefore composites important review approaches 201 202 indexes analysis andor functions system 20 2

RESUMO: Efeitos de extratos vegetais têm sido apontados e comprovados em pesquisas sob diferentes aplicações na agricultura, incluindo o tratamento de sementes. A eficácia de extratos vegetais é atribuída a presença de certas classes de compostos, logo, sendo importante estudos que visam a identificação e quantificação destes compostos presentes em extratos de plantas usados na agricultura, bem como para o tratamento de sementes. Objetivou-se compreender e descrever, por meio de uma revisão sistematizada, quais as principais abordagens realizadas, classes e compostos identificados em estudos com extratos vegetais para diferentes aplicações na agricultura e no tratamento de sementes. O período 2015 a 2020 foi o que apresentou os maiores índices de publicações de pesquisas que exploram extratos vegetais na agricultura, buscando identificar e, ou quantificar os compostos presentes nos extratos vegetais. Os compostos fenólicos constituem a classe prioritária de metabólitos para diferentes funções, efeitos e aplicações na agricultura, principalmente no tratamento de sementes. Os terpenos apresentam grande potencial como bioinseticidas para a agricultura. Espécies de plantas ricas em compostos fenólicos e terpenos são fontes potenciais para controle alternativo na proteção de sistemas produtivos. RESUMO sementes logo Objetivouse Objetivou se descrever sistematizada realizadas 201 202 funções produtivos 20 2

ABSTRACT Purpose: To compare the long-term ocular findings of children that were operated of congenital cataract before the age of two and that received an intraoperative intracameral triamcinolone injection or used postoperative oral prednisolone to modulate ocular inflammation. Methods: All patients who had previously participated in a clinical trial that analyzed the 1-year surgical outcomes of congenital cataract surgery utilizing intracameral triamcinolone (study group) or oral prednisolone (control group) were eligible to participate in this prospective cohort research. Patients’ medical records were reviewed, and the children underwent a complete ophthalmologic exam on final follow-up. Biomicroscopic findings, intraocular pressure, central corneal thickness, the need for additional surgical interventions, and findings compatible with glaucoma were the primary end measures. Results: Twenty-six eyes (26 patients) were included (study group = 11 eyes; control group = 15 eyes). The mean follow--up was 8.2 ± 1.2 years and 8.1 ± 1.7 years in the study and control groups, respectively (p=0.82). All eyes presented a centered intraocular lens. There was no statistically significant difference between the groups with regards to the presence of posterior synechia (p=0.56), intraocular pressure (p=0.49), or central corneal thickness (p=0.21). None of the eyes fulfilled the glaucoma diagnostic criteria, presented secondary visual axis obscuration, or were reoperated. Conclusion: The long--term ocular findings of children that underwent congenital cataract surgery and received an intraoperative intracameral triamcinolone injection were similar to those that used postoperative oral prednisolone to modulate ocular inflammation. This suggests that intracameral triamcinolone may substitute oral prednisolone in congenital cataract surgery, facilitating the postoperative treatment regimen and compliance. Purpose longterm long term inflammation Methods 1year year 1 research Patients reviewed followup. followup follow up. up follow-up interventions measures Results Twentysix Twenty six 26 (2 eyes. . eyes) 82 8 2 8. 12 1. 81 17 7 p=0.82. p082 p p=0.82 0 (p=0.82) lens p=0.56, p056 p=0.56 , 56 (p=0.56) p=0.49, p049 p=0.49 49 (p=0.49) p=0.21. p021 p=0.21 21 (p=0.21) criteria obscuration reoperated Conclusion compliance ( p08 p=0.8 (p=0.82 p05 p=0.5 5 (p=0.56 p04 p=0.4 4 (p=0.49 p02 p=0.2 (p=0.21 p0 p=0. (p=0.8 (p=0.5 (p=0.4 (p=0.2 p=0 (p=0. p= (p=0 (p= (p

RESUMO Objetivo: Comparar os achados oculares em longo prazo de crianças que se submeteram à cirurgia de catarata congênita antes dos dois anos de idade e receberam uma injeção intracameral de triancinolona no intraoperatório ou usaram prednisolona oral no pós-operatório para modular a inflamação ocular. Métodos: Neste estudo prospectivo de coorte, todos os pacientes que participaram de um ensaio clínico anterior, que analisou os resultados cirúrgicos de 1 ano da cirurgia de catarata congênita usando triancinolona intracameral (Grupo de Estudo) ou prednisolona oral (Grupo Controle), eram elegíveis para participar. Os prontuários médicos dos pacientes foram revisados e as crianças foram submetidas a um exame oftalmológico completo no acompanhamento final. As principais medidas de desfecho foram: achados biomicroscópicos, pressão intraocular, espessura central da córnea, a necessidade de intervenções cirúrgicas adicionais e achados compatíveis com glaucoma. Resultados: Vinte e seis olhos (26 pacientes) foram incluídos (Grupo de Estudo = 11 olhos; Grupo de Controle = 15 olhos). O seguimento médio foi de 8,2 ± 1,2 anos e 8,1 ± 1,7 anos nos Grupos de Estudo e Controle, respectivamente (p=0,82). Todos os olhos apresentavam lente intraocular centrada. Não houve diferença estatisticamente significativa entre os grupos com relação à presença de sinéquia posterior (p=0,56), pressão intraocular (p=0,49) ou espessura central da córnea (p=0,21). Nenhum dos olhos preencheu os critérios diagnósticos para glaucoma, apresentou opacificação secundária do eixo visual ou foi reoperado. Conclusão: Os achados oculares em longo prazo de crianças que se submeteram à cirurgia de catarata congênita e receberam uma injeção intracameral de triancinolona no intraoperatório foram semelhantes aos que usaram prednisolona oral no pós-operatório para modular a inflamação ocular, sugerindo que a triancinolona intracameral pode substituir a prednisolona oral na cirurgia de catarata congênita, facilitando o tratamento pós-operatório e a adesão ao mesmo. Objetivo pósoperatório pós operatório ocular Métodos coorte anterior , Controle) participar final biomicroscópicos glaucoma Resultados 26 (2 olhos. . olhos) 82 8 2 8, 12 1, 81 17 7 p=0,82. p082 p p=0,82 0 (p=0,82) centrada p=0,56, p056 p=0,56 56 (p=0,56) p=0,49 p049 49 (p=0,49 p=0,21. p021 p=0,21 21 (p=0,21) reoperado Conclusão mesmo ( p08 p=0,8 (p=0,82 p05 p=0,5 5 (p=0,56 p=0,4 p04 4 (p=0,4 p02 p=0,2 (p=0,21 p0 p=0, (p=0,8 (p=0,5 (p=0, (p=0,2 p=0 (p=0 p= (p= (p

ABSTRACT: The present study evaluated the effect of increasing levels of wet brewery residue (WBR) as a source of roughage on nutrient intake, performance and ingestive behaviour in lambs finished in confinement. Thirty-two uncastrated male lambs were used, weaned at 50 days of age. The treatments comprised increasing levels of WBR as roughage in the diet: 31, 44, 57 and 70%, on a dry-matter. The concentrate consisted of disintegrated corn, soybean meal, calcitic limestone and mineral salt. There was a quadratic effect (P ≤ 0.05) from the increasing levels of WBR on the intake of DM, OM, CP and TCH. The intake of EE, NDF and ADF increased linearly (P ≤ 0.01), while TDN intake showed a linear reduction (P < .0001) for increases in the level of WBR. There was a quadratic effect (P ≤ 0.010) on the average daily weight gain (ADG) from the addition of WBR to the diet. The time spent on feeding and rumination (P ≤ 0.05), expressed in minutes, showed a linear increase. Feeding rate for DM showed decreasing linear behaviour (P ≤ 0.006), while NDF rumination rate showed increasing linear behaviour (P ≤ 0.013) due to the increased levels of WBR in the diet. There was a linear increase in the time spent on each feeding activity (P ≤ 0.007) with the increase in WBR in the diet. Maximum weight gain is achieved when 46.9% WBR is used as a source of roughage in the diet, and this level can be recommended for terminating lambs in confinement. ABSTRACT (WBR confinement Thirtytwo Thirty two 5 age diet 31 44 70 70% drymatter. drymatter dry matter. matter dry-matter corn meal salt P 0.05 005 0 05 OM TCH EE 0.01, 001 0.01 , 01 0.01) .0001 0001 0.010 0010 010 ADG (ADG 0.05, minutes 0.006, 0006 0.006 006 0.006) 0.013 0013 013 0.007 0007 007 469 46 9 46.9 3 4 7 0.0 00 .000 000 0.00 46. 0. .00 .0 .

RESUMO: O objetivo do presente estudo foi avaliar o efeito de níveis crescentes de resíduo úmido de cervejaria (RUC) como fonte de volume sobre o consumo de nutrientes, desempenho e comportamento ingestivo de cordeiros terminados em confinamento. Foram utilizados 32 cordeiros machos não castrados, desmamados aos 50 dias de idade. Os tratamentos consistiram em níveis crescentes de RUC como volumoso na dieta: 31, 44, 57 e 70%, na matéria seca. O concentrado consistia em milho desintegrado, farelo de soja, calcário calcítico e sal mineral. Houve efeito quadrático (P ≤ 0,05) dos níveis crescentes de RUC sobre o consumo de MS, MO, PB e CHT. O consumo de EE, FDN e FDA aumentou linearmente (P < .0001), enquanto o consumo de NDT apresentou redução linear (P < .0001) para aumentos no nível de WBR. Houve um efeito quadrático (P ≤ 0,010) no ganho de peso médio diário (GMD) da adição de RUC à dieta. O tempo gasto com alimentação e ruminação (P ≤ 0,05) e para mastigação total (P ≤ 0,003), expresso em minutos, apresentou aumento linear. A taxa de alimentação para MS apresentou comportamento linear decrescente (P ≤ 0,006), enquanto a taxa de ruminação da FDN apresentou comportamento linear crescente (P ≤ 0,013) devido ao aumento dos níveis de RUC na dieta. Houve aumento linear no tempo gasto em cada atividade alimentar (P ≤ 0,007) com o aumento da RUC na dieta. O ganho de peso máximo é alcançado quando 46,9% de RUC é usado como fonte de volumoso na dieta, e este nível pode ser recomendado para cordeiros terminados em confinamento. RESUMO (RUC nutrientes confinamento 3 castrados 5 idade dieta 31 44 70 70% seca desintegrado soja mineral P 0,05 005 0 05 MO CHT EE .0001, 0001 .0001 , WBR 0,010 0010 010 GMD (GMD 0,003, 0003 0,003 003 0,003) minutos 0,006, 0006 0,006 006 0,006) 0,013 0013 013 0,007 0007 007 469 46 9 46,9 4 7 0,0 00 000 .000 0,01 001 01 0,00 46, 0, .00 .0 .

ABSTRACT Introduction Instruments to manage adverse effects of endocrine therapy with Aromatase inhibitors (AI) may improve adherence and persistence to treatment and Health-Related Quality of Life (HRQL). The 31-item Cervantes Scale (CS-31) is an HRQL questionnaire with particularities of the perimenopausal and postmenopausal period that could be an appropriate instrument to assess HRQL in Breast Cancer (BC) survivors. Objective This study aimed to perform additional validation of the CS-31 for BC survivors undergoing adjuvant endocrine therapy. Methods This prospective study was performed at three time points named T0, T1, and T2: initial, intermediate, and final follow-up period, respectively, totaling 24 months of follow-up. At each time point, the participants completed the CS-31, Functional Assessment of Chronic Illness Therapy - Fatigue (FACIT-F), and Hospital Anxiety and Depression Scale (HADS). The internal consistency, construct validity, responsiveness analyses, and known-group validity of CS-31 were evaluated. Results This study included 89 postmenopausal women diagnosed with hormone receptor-positive early BC in adjuvant endocrine therapy with AI. The internal consistency was good (Cronbach's alpha = 0.89). Construct validity received a positive rating, with 100% of results consistent with prior hypotheses. A prospective improvement in HRQL was identified for the CS-31 Global Score and FACIT-F Total Score and for most of their domains. Furthermore, women with anxiety and depression by HADS presented worse HRQL by CS-31. Conclusion The authors identified that the CS-31 seems to be appropriate for use in oncology medical routine and may help to monitor adverse effects and HRQL of BC survivors during adjuvant endocrine therapy. AI (AI HealthRelated Health Related HRQL. . (HRQL) 31item item 31 CS31 CS (CS-31 (BC CS-3 T0 T T1 T2 initial intermediate followup follow up respectively 2 followup. up. point CS31, 31, FACITF, FACITF FACIT F , (FACIT-F) HADS. (HADS) analyses knowngroup known group evaluated 8 receptorpositive receptor Cronbachs Cronbach s 0.89. 089 0.89 0 0.89) rating 100 hypotheses domains Furthermore CS31. 31. (HRQL 3 CS3 (CS-3 CS- (FACIT-F (HADS 08 0.8 10 (CS- 0. 1 (CS

Abstract Integrative network analysis (INA) is important for identifying gene modules or epigenetically regulated molecular pathways in diseases. This study evaluated the effect of excessive gestational weight gain (EGWG) on INA of differentially methylated regions, maternal metabolism and offspring growth. Brazilian women from “The Araraquara Cohort Study” with adequate pre-pregnancy body mass index were divided into EGWG (n=30) versus adequate gestational weight gain (AGWG, n=45) groups. The methylome analysis was performed on maternal blood using the Illumina MethylationEPIC BeadChip. Fetal-neonatal growth was assessed by ultrasound and anthropometry, respectively. Maternal lipid and glycemic profiles were investigated. Maternal triglycerides-TG (p=0.030) and total cholesterol (p=0.014); fetus occipito-frontal diameter (p=0.005); neonate head circumference-HC (p=0.016) and thoracic perimeter (p=0.020) were greater in the EGWG compared to the AGWG group. Multiple linear regression analysis showed that maternal DNA methylation was associated with maternal TG and fasting insulin, fetal abdominal circumference, and fetal and neonate HC. The DMRs studied were enriched in 142 biological processes, 21 molecular functions,and 17 cellular components with terms directed for the fatty acids metabolism. Three DMGMs were identified:COL3A1, ITGA4 and KLRK1. INA targeted chronic diseases and maternal metabolism contributing to an epigenetic understanding of the involvement of GWG in maternal metabolism and fetal-neonatal growth. (INA (EGWG regions Study prepregnancy pre pregnancy n=30 n30 n 30 (n=30 AGWG, (AGWG n=45 n45 45 groups BeadChip Fetalneonatal Fetal neonatal anthropometry respectively investigated triglyceridesTG triglycerides p=0.030 p0030 p 0 030 (p=0.030 p=0.014 p0014 014 (p=0.014) occipitofrontal occipito frontal p=0.005 p0005 005 (p=0.005) circumferenceHC circumference HC p=0.016 p0016 016 (p=0.016 p=0.020 p0020 020 (p=0.020 group insulin 14 processes 2 functionsand functions 1 identifiedCOL3A1, identifiedCOL3A1 identifiedCOLA identified COL3A1, COL3A1 COL A identified:COL3A1 ITGA KLRK1 KLRK fetalneonatal n=3 n3 3 (n=3 n=4 n4 4 p=0.03 p003 03 (p=0.03 p=0.01 p001 01 (p=0.014 p=0.00 p000 00 (p=0.005 (p=0.01 p=0.02 p002 02 (p=0.02 identifiedCOL identifiedCOL3A COLA COL3A identified:COL3A n= (n= p=0.0 p00 (p=0.0 (p=0.00 (n p=0. p0 (p=0. p=0 (p=0 p= (p= (p

RESUMEN Objetivo: describir el proceso de desarrollo, validación y evaluación de una cartilla educativa para prevenir el acoso escolar transfóbico. Método: se trata de un estudio metodológico, realizado de febrero a diciembre de 2022 en una escuela pública de João Pessoa, Paraíba, Brazil. La cartilla educativa fue elaborada de acuerdo con la trayectoria metodológica propuesta por Echer, y sometida a validación de contenido y evaluación semántica por jueces expertos y docentes de la Escuela Primaria II, respectivamente. En el análisis de los datos, se utilizaron los coeficientes de validez de contenido y de correlación intraclase, y el índice de concordancia semántica. Resultados: la cartilla educativa tuvo su contenido validado con un coeficiente de validez de 0,981 y un coeficiente de correlación intraclase de 0,833 para el conjunto de ítems del instrumento de evaluación. En la evaluación semántica, los docentes consideraron comprensible el cuadernillo, con un nivel mínimo de acuerdo del 94%. Conclusión: la cartilla desarrollada fue considerada válida por los jueces, para ser utilizada con docentes, de manera individual y en acciones de educación continua o de salud, con el fin de contribuir a la prevención del acoso escolar transfóbico en la escuela. Objetivo desarrollo Método metodológico 202 Pessoa Paraíba Brazil Echer II respectivamente datos Resultados 0981 0 981 0,98 0833 833 0,83 cuadernillo 94 94% Conclusión salud 20 098 98 0,9 083 83 0,8 9 2 09 0, 08 8

ABSTRACT Objective: to describe the process of developing, validating and assessing an educational booklet to prevent transphobic bullying at school. Method: this is a methodological study, carried out from February to December 2022 at a public school in João Pessoa, Paraíba, Brazil. The educational booklet was developed in accordance with the methodological trajectory proposed by Echer, and submitted to content validity and semantic assessment by expert judges and Elementary School II teachers, respectively. In data analysis, content validity and Intraclass Correlation Coefficients were used, in addition to semantic agreement index. Results: the educational booklet had its content validated with a validity coefficient of 0.981 and an Intraclass Correlation Coefficient of 0.833 for the set of items in the assessment instrument. In semantic assessment, teachers considered the booklet understandable, with a minimum agreement level of 94%. Conclusion: the booklet developed was considered valid by judges, to be used with teachers, individually and in continuing education or health actions, in order to contribute to preventing transphobic bullying at school. Objective developing Method study 202 Pessoa Paraíba Brazil Echer respectively analysis index Results 0981 0 981 0.98 0833 833 0.83 instrument understandable 94 94% Conclusion actions 20 098 98 0.9 083 83 0.8 9 2 09 0. 08 8

RESUMO Objetivo: Descrever o processo de desenvolvimento, validação e avaliação de cartilha educacional para prevenção do bullying transfóbico na escola. Método: Trata-se de um estudo metodológico, realizado no período de fevereiro a dezembro de 2022 em uma escola pública de João Pessoa, Paraíba, Brasil. A cartilha educacional foi desenvolvida de acordo com a trajetória metodológica proposta por Echer, e submetida à validação de conteúdo e avaliação semântica por juízes especialistas e professores do Ensino Fundamental II, respectivamente. Na análise dos dados, utilizaram-se os coeficientes de validade de conteúdo e correlação intraclasse, e o índice de concordância semântica. Resultados: A cartilha educacional teve seu conteúdo validado com coeficiente de validade de 0,981 e coeficiente de correlação intraclasse de 0,833 para o conjunto de itens do instrumento de avaliação. Na avaliação semântica, os professores consideraram a cartilha compreensível, com nível de concordância mínima de 94%. Conclusão: A cartilha desenvolvida foi considerada válida por juízes, para ser utilizada com professores, de forma individual e em ações de educação permanente ou em saúde, a fim de contribuir na prevenção do bullying transfóbico na escola. Objetivo desenvolvimento Método Tratase Trata se metodológico 202 Pessoa Paraíba Brasil Echer II respectivamente dados utilizaramse utilizaram Resultados 0981 0 981 0,98 0833 833 0,83 compreensível 94 94% Conclusão saúde 20 098 98 0,9 083 83 0,8 9 2 09 0, 08 8

ABSTRACT. Given the importance of dementia syndrome and its impacts on the population, interest in studying modifiable risk factors for dementia is growing. Objective: To compare the prevalence of risk factors for dementia in middle-aged and older adults over a two-year period and to identify what variables in baseline were predictive of cognitive decline in the follow-up. Methods: Longitudinal and quantitative study, with follow-up evaluation after two years, conducted with 200 participants aged 45 years or more, registered in Primary Care Units. In the baseline (2018/2019) and follow-up (2021) assessments, sociodemographic data were collected, and cognitive performance and risk factors for dementia were evaluated (education, hearing loss, head trauma, high blood pressure, alcohol use, obesity, smoking, depressive symptoms, social isolation, physical inactivity, and diabetes mellitus). Data were compared using the McNemar’s test. Individual multinomial logistic regression models were performed to identify the factors associated with cognitive decline after two years. Results: The percentages of low education, traumatic brain injury, and smoking remained the same in both assessments. There was a significant increase in the prevalence of high blood pressure (from 55.0 to 62.0%) and physical inactivity (from 58.5 to 74.5%) and a significant reduction in social isolation (from 25.0 to 18.0%). Participants with depressive symptoms in baseline had a higher risk of cognitive decline in follow-up. Conclusion: There was an increase in the prevalence of high blood pressure and physical inactivity and a reduction in social isolation after two years. Depressive symptoms predict cognitive decline. ABSTRACT population growing Objective middleaged middle twoyear year followup. followup follow up. up Methods study 20 4 more Units 2018/2019 20182019 2018 2019 (2018/2019 2021 (2021 assessments collected education (education loss trauma use obesity mellitus. mellitus . mellitus) McNemars McNemar s test Results injury from 550 55 0 55. 62.0% 620 62 585 58 5 58. 74.5% 745 74 250 25 25. 18.0%. 180 18.0% 18 18.0%) Conclusion 2 2018/201 2018201 201 (2018/201 202 (202 62.0 6 74.5 7 18.0 1 2018/20 201820 (2018/20 (20 62. 74. 18. 2018/2 20182 (2018/2 (2 2018/ (2018/ ( (2018 (201

RESUMO. Dada a importância da síndrome demencial e de seus impactos na população, cresce o interesse em estudar os fatores de risco modificáveis para a demência. Objetivo: Comparar a prevalência de fatores de risco para demência em adultos de meia-idade e pessoas idosas, em um período de dois anos, e identificar quais fatores de risco na avaliação de base predizem o declínio cognitivo na avaliação de acompanhamento. Métodos: Estudo longitudinal e quantitativo, com avaliação de acompanhamento após dois anos, realizado com 200 participantes com 45 anos ou mais, cadastrados na Atenção Básica. Nas avaliações de base (2018/2019) e de acompanhamento (2021) foram coletados dados sociodemográficos, foram feitas avaliação cognitiva e avaliação dos fatores de risco para demência (escolaridade, perda auditiva, traumatismo craniano, hipertensão arterial, uso de álcool, obesidade, tabagismo, sintomas depressivos, isolamento social, inatividade física, diabetes mellitus). Os dados foram comparados pelo teste de McNemar. Modelos de regressão logística multinominal individuais foram conduzidos para identificar quais fatores estavam associados ao declínio cognitivo após dois anos. Resultados: Os percentuais de baixa escolaridade, traumatismo craniano e tabagismo mantiveram-se os mesmos nas duas avaliações. Houve aumento significativo da prevalência de hipertensão arterial (de 55,0 para 62,0%) e inatividade física (de 58,5 para 74,5%), e redução significativa do isolamento social (de 25,5 para 18,0%). Participantes com sintomas depressivos na avaliação de base apresentaram maior risco de declínio cognitivo no acompanhamento. Conclusão: Houve aumento das prevalências de hipertensão arterial e inatividade física; e redução do isolamento social. Sintomas depressivos predizem declínio cognitivo. RESUMO população Objetivo meiaidade meia idade idosas Métodos quantitativo 20 4 mais Básica 2018/2019 20182019 2018 2019 (2018/2019 2021 (2021 sociodemográficos escolaridade (escolaridade auditiva álcool obesidade mellitus. mellitus . mellitus) McNemar Resultados mantiveramse mantiveram se 550 55 0 55, 62,0% 620 62 585 58 5 58, 74,5%, 745 74,5% , 74 74,5%) 255 25 25, 18,0%. 180 18,0% 18 18,0%) Conclusão 2 2018/201 2018201 201 (2018/201 202 (202 62,0 6 74,5 7 18,0 1 2018/20 201820 (2018/20 (20 62, 74, 18, 2018/2 20182 (2018/2 (2 2018/ (2018/ ( (2018 (201

Abstract This paper discusses the potential of gamification as a tool for teaching and learning in geotechnical engineering. Gamification involves incorporating elements of gameplay such as challenges, rewards, competition, and cooperation into teaching and learning environments to make the process more interactive and engaging. Although gamification is widely used in many fields, it is still relatively new in geotechnical engineering. This paper presents the ‘Soil Character’ board game developed by the GeoFUN group as an example of successful gamification in geotechnical engineering education. The game focuses on basic soil characterization, including soil classification systems, index properties, and geotechnical characterization tests such as sieving, sedimentation, and Atterberg limits. The paper provides background information on the development of the game, and a description of the game components. The online Portuguese version of the game was tested with eight civil engineering undergraduate students who had successfully undertaken the introductory soil mechanics module. Student’s satisfaction in terms of game design, rules, and gameplay was measured via a questionnaire. Results of the questionnaires showed that the game was well evaluated in all aspects. Student volunteers reported that they felt very motivated, and that they wished they had been able to play the game when they were learning the topic. Thus, results presented in this paper suggest that gamification has the potential to make geotechnical engineering education more interactive and engaging. Exploring the effectiveness of the game in various contexts and with diverse student populations constitutes a key direction for our future research. challenges rewards competition engaging fields Soil Character systems properties sieving sedimentation limits components module Students s design rules questionnaire aspects motivated topic Thus research

Abstract We announce the mitochondrial genomes of seven species of the genus Sporophila (S. bouvreuil, S. iberaensis, S. melanogaster, S. minuta, S. nigrorufa, S. pileata, and S. ruficollis) which were validated by comparative genomic and phylogenetic analysis with related species. The mitochondrial genomes of seven passerines of the genus Sporophila were assembled (three complete and four nearly complete genomes) and were validated by reconstructing phylogenetic relations within Thraupidae. The complete mitogenomes ranged from 16,781 bp in S. ruficollis to 16,791 bp in S. minuta. We identified a conserved genome composition within all mitogenomes with 13 protein-coding genes, 22 tRNAs and two rRNAs. We observed a bias in the nucleotide composition and six mutational hotspots in Sporophila mitogenomes. Our mitogenome-based phylogenetic tree has S. minuta, S. maximiliani and S. nigricollis as sister species of the remaining species in the genus. We present new mitogenome sequences for seven Sporophila species, providing new genomic resources that may be useful for research on the evolution, comparative genetics, and conservation of this threatened group. S (S bouvreuil iberaensis melanogaster minuta nigrorufa pileata three Thraupidae 16781 16 781 16,78 16791 791 16,79 1 proteincoding protein coding genes 2 rRNAs mitogenomebased based evolution genetics group 1678 78 16,7 1679 79 167 7 16,

Abstract Brazil has emerged as the world's largest producer of annatto seeds (Bixa orellana L.). Fortunately, the increase in national production has been accompanied by an improvement in the quality of this raw material. Annatto is a typical crop of small producers who usually use their empirical knowledge to evaluate the quality of the crop. Thefore, the visual observation of the color of annatto seeds has been considered a quality standard by many producers. To evaluate the scientific evidence, the present study evaluated annatto seeds from 51 accessions of the germplasm bank of the Agronomic Institute (Instituto Agronômico de Campinas - IAC) to determine a possible correlation between the pigment’s contents and the color of annatto seeds as well as the influence of humidity on this correlation. The correlations between the concentrations of pigments, humidity, lipids, and color (L*, a*, b*) were also evaluated. The color was evaluated using a colorimeter with measurements of the CIE L*, a*, b* scale. Only the correlation between the variables moisture and lipids was considered strong through the simple correlation, while the variables bixin content and coordinate a* presented a significant correlation while eliminating the effect of moisture (partial correlation). Thus, a correlation between the red color and the bixin concentration of annatto seeds was observed even though it was a weak correlation. In turn, the color difference (ΔEab) between the samples with higher and lower bixin contents was only 1.44. This difference indicates that large variations in the bixin concentrations may lead to small color changes in the annatto seeds, impairing the use of this criterion for quality control of these seeds. worlds world s Bixa L.. L L. . L.) Fortunately material Thefore evidence 5 Instituto IAC pigments pigment (L* b L* scale partial correlation) Thus turn ΔEab (ΔEab 144 1 44 1.44 (L 14 4 1.4 1.

Resumo O Brasil tem se firmado como o maior produtor mundial de sementes de urucum (Bixa orellana L.). Felizmente, o aumento da produção nacional tem sido acompanhado por uma melhoria da qualidade dessa matéria-prima. O urucum é uma cultura típica de pequenos produtores, que em geral usa seu conhecimento empírico para avaliar a qualidade do material que está produzindo. A observação visual da cor das sementes de urucum tem sido considerada padrão de qualidade por muitos produtores. Para avaliar as evidências científicas, o presente estudo avaliou amostras de sementes de urucum de 51 acessos do banco de germoplasma do Instituto Agronômico de Campinas (IAC), para determinar se existe correlação entre as concentrações de pigmentos com a cor das sementes e a influência da umidade nessa correlação. Foram avaliadas também as correlações entre as concentrações de pigmentos, umidade, lipídios e a cor (L*, a*, b*). Para a avaliação da cor, utilizamos um colorímetro com a medida da cor pelo sistema CIE L*, a*, b*. Apenas a correlação simples entre as variáveis Umidade e Lipídios foi considerada forte, pela classificação usada neste estudo. Quando se remove o efeito da Umidade (correlação parcial), apenas as variáveis bixina e a* apresentaram correlação significativa, considerada fraca pela classificação utilizada. Portanto, mesmo considerada fraca, há uma correspondência entre a cor vermelha das sementes de urucum com a concentração de bixina das amostras estudadas. Contudo, a diferença da cor (ΔEab) entre as amostras com maior e menor teor de bixina foi de apenas 1,44. Essa diferença indica que grandes variações nas concentrações de bixina nas sementes de urucum podem levar a pequenas variações de cor, dificultando o uso desse critério para o controle de qualidade desses grãos. Bixa L.. L L. . L.) Felizmente matériaprima. matériaprima matéria prima. prima matéria-prima produtores produzindo científicas 5 IAC, IAC , (IAC) (L* b b* b*) L* forte parcial, parcial parcial) significativa utilizada Portanto estudadas Contudo ΔEab (ΔEab 144 1 44 1,44 grãos (IAC (L 14 4 1,4 1,

ABSTRACT Objective: The prevalent symptoms of severe dengue in pediatric patients are divided into three subgroups: severe plasma leakage, severe bleeding, and severe organ damage. In addition, the seasonal patterns of the disease and the outcomes of cure or death from dengue were evaluated. Methods: An epidemiological, observational, analytical, cross-sectional study was conducted with data from the Notifiable Disease Information System (SINAN - Sistema de Informação de Agravos de Notificação and DATASUS - Departamento de Informática do Sistema Único de Saúde) of the Ministry of Health from 2019 to 2020. Results: During the study period, 1,857 cases of severe dengue were observed in the pediatric age group, with the most common symptoms being respiratory failure, melena, hematemesis, and altered level of consciousness. The total proportion of patients hospitalized for severe dengue was 89.6%, and 51.2% of these patients died, corroborating the importance of early detection of the disease. Conclusion: Severe dengue is more prevalent during the seasonal period, with hot and humid characteristics owing to the mechanism involved in the viral cycle. The most prevalent symptoms of severe dengue in pediatric patients were respiratory failure alone, gastrointestinal bleeding, and altered level of consciousness. It is important to identify signs of severity for early intervention and a better prognosis, considering that death is closely related to a delayed diagnosis. Objective subgroups leakage bleeding damage addition evaluated Methods epidemiological observational analytical crosssectional cross sectional SINAN Saúde 201 2020 Results period 1857 1 857 1,85 group melena hematemesis consciousness 896 89 6 89.6% 512 51 2 51.2 died Conclusion cycle alone prognosis diagnosis 20 202 185 85 1,8 8 89.6 5 51. 18 1, 89.