Abstract Dendrobium nobile is a traditional Chinese medicine and nourishing food in China. Alkaloids and sesquiterpene glycosides are the two main active ingredients in D. nobile. The previous reports showed that alkaloids and sesquiterpene glycosides have the same upstream biosynthetic pathway, starting from sesquiterpene components, part of which is converted to sesquiterpene glycosides by the action of UDP-glycosyltransferase, and part of which is further synthesized to alkaloids. Therefore, sesquiterpene UDP-glycosyltransferases play an important role in the biosynthesis of alkaloids and sesquiterpene glycosides in D. nobile. In this study, the key glycosyltransferase genes in sesquiterpene glycosylation in D. nobile were explored, predicted and validated by transcriptome technology. China D pathway components UDPglycosyltransferase, UDPglycosyltransferase UDP glycosyltransferase, UDP-glycosyltransferase Therefore UDPglycosyltransferases glycosyltransferases study explored technology

Resumen La Bahía de Campeche se encuentra en una región de moderada a alta actividad sísmica relacionada con la triple unión activa entre los límites de las placas de América del Norte, el Caribe y la de Cocos. Por lo tanto, las plataformas fijas en alta mar y las estructuras submarinas en la Bahía de Campeche deben diseñarse contra la carga sísmica. Una base de datos de pruebas de clasificación y propiedades índice, mediciones in situ de la velocidad de la onda de corte Vs utilizando la sonda suspendida, pruebas in situ de piezocono penetrómetro (PCPT), pruebas de columna resonante para caracterizar el módulo de rigidez y la relación de amortiguación del material a bajas deformaciones angulares (10-5% a aproximadamente 0.1 %) y pruebas cíclicas de corte simple directo (DSS) con deformación controlada para evaluar la disminución del módulo de rigidez y el aumento de la relación de amortiguación del material a deformaciones angulares altas (0.1 % a aproximadamente 10 %) realizados en la arena de la Bahía de Campeche incluyendo arenas sin contenido de carbonato hasta el 100% del contenido de carbonato recuperados desde el fondo marino y hasta a una profundidad de 120 m por debajo del fondo marino. La base de datos fue diseñada específicamente para desarrollar para las arenas de la Bahía de Campeche correlaciones empíricas para determinar Vs, cuando no se dispone de mediciones in situ de Vs, y modelos numéricos para predecir la variación del módulo de rigidez normalizado (G/Gmax) y la relación de amortiguamiento (D) de materiales en función de la deformación angular (γ) cuando no hay resultados de pruebas dinámicas disponibles para todas las capas de arena. Las ecuaciones desarrolladas para calcular Vs, y las curvas de G/Gmax-γ y D-γ de las arenas de la Bahía de Campeche se recomiendan para evaluaciones preliminares o tal vez incluso finales de respuesta sísmica del sitio.

Abstract The Bay of Campeche is located in a region of moderate to high seismic activity related to the active triple junction between the North American, Caribbean, and Cocos plate boundaries. Therefore, the fixed offshore platforms and subsea structures in the Bay of Campeche must be designed against earthquake loading. A database was developed of classification and index properties tests, in situ measurements of shear wave velocity (Vs) using downhole P-S suspension seismic velocity logging, in situ piezocone penetration tests, resonant column tests to characterize the shear modulus and material damping ratio at small shear strains (10−5 % to about 0.1 %), and strain-controlled cyclic direct simple shear tests to evaluate the decrease of shear modulus and the increase of material damping ratio at large shear strains (0.1 % to about 10 %) performed on sand from the Bay of Campeche, including sands with no carbonate content to 100 % carbonate content, retrieved from the seafloor to a penetration depth of 120 m below seafloor. The database was tailored specifically to develop empirical correlations for the Bay of Campeche sand to determine Vs when in situ measurements of Vs are not available and to develop numerical modeling to predict the variation of the normalized shear modulus (G/Gmax) and material damping ratio (D) as a function of shear strains (g) when dynamic test results are unavailable for all the sand layers. The equations developed to calculate Vs and the curves of G/Gmax-γ and D-γ of Bay of Campeche sands are recommended for preliminary or perhaps even final seismic site response evaluations.

ABSTRACT Objective: To compare health-related quality of life (HRQoL) of patients primarily treated with a no-touch saphenous vein graft with that of patients who received a conventional graft. Methods: The study included all individuals treated with a percutaneous coronary intervention (PCI) on a saphenous vein graft (SVG) between January 2006 and June 2020. The RAND-36 health survey was used to assess HRQoL. The Mann-Whitney U test was used to test differences in HRQoL between the two groups. Effect size was estimated via Cohen’s d. The average treatment effect between the groups was tested by propensity score matching (PSM). Results: Of the 346 patients treated with a PCI in a stenosed or occluded SVG, 165 responded to RAND-36 (no-touch: n=48; conventional: n=117). Patients with a no-touch graft reported better mean values on seven of the eight health survey domains. Statistically significant differences were observed for four of the domains, all in favour of the no-touch group. The effect size estimates indicated a small difference for five domains, with the highest values (>0.40) seen for the general health and energy/fatigue domains. PSM confirmed a statistically significant difference for the physical functioning and general health domains. Conclusion: At a mean follow-up of 5.4 years, patients who received a PCI in no-touch vein grafts showed significantly better HRQoL than those who received a PCI in conventional vein grafts. Objective healthrelated related (HRQoL notouch no touch Methods (PCI SVG (SVG 200 2020 RAND36 RAND 36 RAND-3 MannWhitney Mann Whitney Cohens Cohen s d PSM. . (PSM) Results 34 16 (no-touch n=48 n48 n 48 n=117. n117 n=117 117 n=117) domains group >0.40 040 0 40 (>0.40 energyfatigue energy fatigue Conclusion followup follow up 54 5 4 5. years 20 202 RAND3 3 RAND- (PSM 1 n=4 n4 n11 n=11 11 >0.4 04 (>0.4 2 n= n1 n=1 >0. (>0. >0 (>0 > (> (

ABSTRACT Background: Diameter distribution models are one of the most important components of growth and yield models. Diameter distribution models, based on the Weibull function, were developed for even-aged mixed-oak stands (Turkey oak, Sessile oak, and Hungarian oak) in northwestern Turkey. Two modeling methods were considered. Weibull parameters were recovered from either equation predicting Dq and Dvar (method of moments) or equations predicting Dq and D 90 (hybrid method). For each modeling method, three estimation methods were considered: (a) Least Squares method, (b) CDF Regression method in which regression coefficients were estimated separately for each species, and (c) CDF Regression method in which regression coefficients were simultaneously estimated for all species. Results: Results indicated that the hybrid method coupled with the CDF Regression estimation method yield best results in this study. Similar results were obtained when the regression coefficients were estimated either separately for each species or simultaneously for all species. Conclusion: The proposed models enable one to predict diameter distribution of a given mixed-oak species stand in northwestern Turkey, using limited stand information. These models are useful tools for the inventory and management of mixed-oak stands.

ABSTRACT Chicken abdominal fat (AF) is an economically important trait, and many studies have been conducted on genetic selection for AF. However, previous studies have focused on detecting functional chromosome mutations or regions using gene chips. The present study used the specific-locus amplified fragment sequencing (SLAF-seq) technology to perform a genome-wide association study (GWAS) on purebred Wengshang Barred chicken. A total of 1,286,715 single-nucleotide polymorphisms (SNPs) were detected, and 175,211 SNPs were selected as candidate SNPs for genome-wide association analysis using TASSEL general linear models. Two SNPs markers reached genome-wide significance. Of these, rs7943847, rs127627362 were significantly associated with AF at 120 days. These SNPs are close to eight genes (SLC16A6, ARSG, WIPI1, PRKAR1A, FAM20A, ABCA8, ABCA9, CPQ,). These results would enrich the studies on AF and promote the use of Chinese chicken, especially the Wenshang Barred chicken.

ABSTRACT The effects of plant essential oil (PEO) on the production performance and immune function of laying hens were evaluated to provide theoretical basis for promoting the natural plant extracts. Eight thousand 1-day-old healthy laying hens were randomly divided into a control group or PEO group, with four replicates per treatment and 1000 hens per replicate. The PEO diet was supplemented with 3g/kg plant extract. Diets were fed for 56 days. The tibia length and keel length were detected on an empty stomach at the end of the trial. Blood samples were collected on the 28th and 56th days to detect the level of C3, C4, IL-1, IL-2, IL-17 and immunoglobulin in the serum. The results showed that, compared with the control, PEO supplementation significantly increased the weight gain rate (WGR) at the 2nd, 4th, 5th and 7th week (p<0.05), and decreased the WGR at the 3rd and 6th week. The tibial length was significantly increased at the 3rd, 5th, 6th and 7th week (p<0.01), and also the keel length at the 5th and 7th week in PEO group. The concentration of IgG and IgM also significantly influenced with PEO supplementation, but there was no significant difference in the complements, C3 and C4, and the IL levels between days 28 and 56. Moreover, no significant difference was observed in body weight and immune organ on day 56. Therefore, we conclude that the addition of PEO could improve the production performance and immune function in laying hens.

Abstract Background: Thrombocytopenia is a hallmark of postdiarrhoeal haemolytic uraemic syndrome (D+ HUS), although it can be transient and therefore undetected. There is scarce information regarding the prevalence and the course of the disease in children with D+ HUS without thrombocytopenia. Objective: To determine the prevalence of D+ HUS without thrombocytopenia and to describe the clinical characteristics of a series of children with this condition. Patients and methods: The medical records of patients with D+ HUS hospitalised between 2000 and 2016 were reviewed to identify those without thrombocytopenia (>150,000 mm3). Demographic, clinical and laboratory parameters of the selected cases were collected and descriptively analysed. Results: Nine cases (5.6%) without thrombocytopenia were identified among 161 patients hospitalised during the study period. Median age at diagnosis was 17 months (7-32) and median prodromal symptom duration was 15 days (7-21). Eight patients maintained normal urine output while the remaining one required dialysis. No patient presented with severe extrarenal compromise and/or hypertension. Conclusions: The prevalence of non-thrombocytopenic D+ HUS was 5.6% and most cases occurred with mild forms of the disease; however, the need for dialysis in one of them indicated that normalisation of platelet count is not always an accurate marker for disease remittance. Our results also confirm that the time of onset of D+ HUS in patients without thrombocytopenia is usually delayed with respect to the initial intestinal symptoms; thus, heightened diagnostic suspicion is necessary.

Resumen Antecedentes: La presencia de trombocitopenia es una marca distintiva del síndrome urémico hemolítico asociado a diarrea (SUH D+); sin embargo, puede ser transitoria y, por lo tanto, no ser detectada. Existe limitada información sobre la prevalencia y el curso de la enfermedad en niños con SUH D+ sin trombocitopenia. Objetivo: Determinar la prevalencia de SUH D+ sin trombocitopenia y describir las características clínicas de una serie de niños con esta particularidad. Pacientes y métodos: Fueron revisadas las historias clínicas de los pacientes con SUH D+ internados entre 2000 y 2016 para identificar a aquellos sin trombocitopenia (>150.000 mm3). De los casos seleccionados se recolectaron las variables demográficas, clínicas y de laboratorio, las cuales fueron analizadas descriptivamente. Resultados: De 161 pacientes internados durante el periodo de estudio se identificaron 9 sin trombocitopenia (5,6%). La mediana de la edad al diagnóstico fue de 17 meses (7-32) y la de la duración del periodo prodrómico, de 15 días (7-21). Ocho pacientes mantuvieron diuresis normal y uno requirió diálisis. Ningún paciente presentó compromiso extrarrenal severo y/o hipertensión arterial. Conclusiones: La prevalencia de SUH D+ sin trombocitopenia fue del 5,6% y la mayoría de los casos fueron leves; sin embargo, el requerimiento de diálisis en uno de ellos señala que la normalización del recuento de plaquetas no siempre es un marcador preciso de resolución de la enfermedad. Nuestros resultados también confirman que el momento de presentación de los pacientes con SUH D+ sin trombocitopenia está usualmente alejado de los primeros síntomas intestinales, por lo que es necesario un alto índice de sospecha diagnóstica.

Background: Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium. Results: Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first constructed with the origins of replication from the chromosome and two native plasmids (pZZM401 and pZZM402) of Z. mobilis ZM4, respectively. The three shuttle vectors were stable in Z. mobilis ZM4 and have 3,32 and 27 copies, respectively. The promoter Ppdc (a), from the pyruvate decarboxylase gene, was clonedinto the shuttle vectors, generatingthe expressionvectors pSUZM1(2, 3)a. The codon-optimized glucoamylase gene from Aspergillus awamori combined with the signal peptide sequence from the alkaline phosphatase gene of Z. mobilis was cloned into pSUZM1(2, 3)a, resulting in the plasmids pSUZM1a-GA, pSUZM2a-GA and pSUZM3a-GA, respectively. After transforming these plasmids into Z. mobilis ZM4, the host was endowed with glucoamylase activity for starch hydrolysis. Both pSUZM2a-GA and pSUZM3a-GA were more efficientatproducingglucoamylase thanpSUZM1a-GA. Conclusions: These results indicated that these expression vectors are useful tools for gene expression in Z. mobilis and this could provide a solid foundation for further studies of heterologous gene expression in Z. mobilis.

Eupatorium adenophorum Spreng., a major invasive weed in southwestern China, has caused great economic losses. In order to find a new herbicide to control E. adenophorum, experiments were conducted to study its physiological and biochemical responses to low and high doses of picloram herbicide (4-amino-3,5,6-trichloropyridine-2-carboxylic acid). Electrolyte leakage, malondialdehyde, and free proline were stimulated by picloram, showing a remarkably increase (p < 0.05) with high herbicide concentration (60, 120, and 240 g ai ha-1). The treated plants exhibited lower osmotic adjustment capacity, high dosage lipid peroxide levels and more free-proline accumulation. It was found that low doses (12 and 24 g ai ha-1) of picloram initially increased catalase, peroxidase, superoxide dismutases and protein, but these indicators decreased (p < 0.05) with the increase of treating time (after 3 d) and dose (120 and 240 g ai ha-1). In addition, the structures of chloroplasts and mitochondria were seriously deformed. These results indicated E. adenophorum can improve its herbicide-tolerance by increasing the antioxidative system activity at the initial period of low picloram stress. However, this protective function disappeared with increasing of treating time and picloram dosage. Eupatorium adenophorum responded differently to low and high concentrations of picloram and ultrastructural changes are an important cause of death in E. adenophorum.

OBJECTIVE: We attempted to evaluate maternal thyroid function in a new self-sequential longitudinal reference interval (SLRI) which we established recently. By this method, we analysed the correlation between pregnancy outcome, neonatal thyroid stimulating hormone (TSH) level and maternal thyroid diseases. METHODS: A total of 1744 pregnant women participated in the study and 1747 babies were born from those women (three bore twins). The levels of TSH, free thyroxine (FT4) and thyroid peroxidase antibodies (TPO-Ab) of mothers were quantified by electrochemistry immunoassay (ECL). The levels of neonatal blood TSH were detected by time-resolved fluorescence immunoassay (TRFIA). All data were collected and statistically analysed by SPSS 13.0 software. RESULTS: With our new SLRI method, we found that 0.11%~3.84% pregnant women would get thyroid diseases. Subclinical hypothyroidism was the most common maternal thyroid disorder. Being positive for thyroid peroxidase antibodies was a significant risk factor of subclinical hypothyroidism during pregnancy. The median, P2.5~P97.5, and interquartile range (IQR) of neonatal TSH (N-TSH) of 1747 babies were 2.72 mIU/L, 0.10~8.01 mIU/L and 2.62 mIU/L, respectively; 28.6% of pregnant women with thyroid diseases developed pregnancy complications. The prevalence was significantly higher than in the normal thyroid function group (p< 0.001). The levels of N-TSH were low correlated with maternal TSH levels (p < 0.05), but there were no significant correlations between N-TSH and maternal FT4 and maternal TPO-Ab (p > 0.05). CONCLUSIONS: Thyroid disorders, especially subclinical hypothyroidism, are common in pregnant women. These disorders are associated with pregnancy and fetal outcome. Routine maternal thyroid function screening is important and should be recommended.

OBJETIVO: Intentamos evaluar la función tiroidea materna en un nuevo intervalo de referencia longitudinal auto-secuencial (SLRI) que establecimos recientemente. Por este método, analizamos la correlación entre el resultado del embarazo, el nivel de la hormona estimulante de la tiroides (TSH) en neonatos, y las enfermedades tiroideas maternas MÉTODOS: Un total de 1744 mujeres embarazadas participó en el estudio y 1747 bebés nacieron de esas mujeres (tres de ellas tuvieron gemelos). Los niveles de TSH, la tiroxina libre (FT4), y los anticuerpos de la peroxidasa tiroidea (TPO-Ab) de las madres, fueron cuantificados mediante inmunoensayo electroquímico (ECL). Los niveles de TSH en la sangre de los neonatos, fueron determinados mediante inmunoensayo por fluorescencia resuelto en el tiempo (TRFIA). Todos los datos fueron recogidos y analizados estadísticamente usando el software SPSS 13.0 RESULTADOS: Con nuestro nuevo método SLRI, encontramos que 0.11%~3.84% de las mujeres embarazadas contraerán enfermedades tiroideas. El hipotiroidismo subclínico fue el trastorno de la tiroides materna más común. Ser positivo a los anticuerpos de la peroxidasa tiroidea fue un factor de riesgo significativo del hipotiroidismo subclínico durante el embarazo. La mediana, P2.5~P97.5, y el rango intercuartil (IQR) de la TSH (N-TSH) neonatal de los 1747 bebés fueron 2.72 mIU/L, 0.10~8.01 mIU/L y 2.62 mIU/L respectivamente. El 28.6% de las mujeres embarazadas que tenían enfermedades tiroideas, desarrollaron complicaciones del embarazo. La prevalencia fue significativamente más alta que en el grupo con función tiroidea normal (p < 0.001). Los niveles de N-TSH fueron bajos en correlación con los niveles de TSH maternos (p < 0.05), pero no hubo ninguna correlación significativa entre la N-TSH y la FT4 materna, y la TPO-Ab materna (p > 0.05). CONCLUSIÓNS: Los trastornos tiroideos, especialmente el hipotiroidismo, son comunes en las mujeres embarazadas.Estos trastornos se hallan asociados con el resultado del embarazo y el resultado fetal. El tamizaje de rutina de la función tiroidea materna es importante y debe recomendarse.

The effects of Dangguibuxue Tang (DBT) on growth performance and immunity response in immunosuppressed broiler chicks were investigated in this study. 240 one-d-old broiler chicks (DaHeng S01) were randomly divided into 4 groups, 2.0% DBT-treatment (A), 0.5% DBT-treatment (B), cyclophosphamide-control (C), and control group (D). From 4 d to 7 d of age, chicks in group A, B and C were given cyclophosphamide (CY) at a dosage of 100mg/kg body weight (BW) daily by intraperitoneal injection to induce immunosuppression. Chicks in group D were given an equal volume of physiological saline daily by intraperitoneal injection and considered normal chicks. Groups A and B were supplemented with 2.0% or 0.5% of DBT in the drinking water from 8 d to 42 d of age. Groups C and D did not receive any additional medication. The results revealed that chicks from group B had lower feed:gain rate (FGR), lower total mortality, higher immunity organ indexes, higher levels of Newcastle disease (ND) antibody and infectious bursal disease (IBD) antibody, higher interleukin-2 and interleukin-6 levels, and greater lymphocyte proliferative responses to concanavalin A (ConA) during the experiment than those from group C. However, no significant difference in the immunity status in the two levels of DBT-treatment was observed. These results indicate that supplementation of 0.5% of DBT can improve both cellular immunity and humoral immunity in immunosuppressed broiler chicks.

La enfermedad injerto vs hospedero (EICH) es un proceso inmunológico, desencadenado por linfocitos T de un donante inmunocompetente, que reaccionan contra los tejidos de un receptor inmunocomprometido. Esto origina daño multiorgánico severo en el agente receptor. Las manifestaciones cutáneas son un signo clínico relevante en períodos tempranos. La EICH postranfusional (EICH-PT) es una entidad poco frecuente y con alta tasa de mortalidad, que se observa en pacientes luego de transfusiones con hemoderivados no irradiados. Los corticoides son el pilar fundamental del tratamiento, una vez instalado el cuadro. La prevención con leucorreducción e irradiación de los componentes sanguíneos, constituye el sustento primordial para evitar su desarrollo. Presentamos un paciente de 5 años de edad, con un diagnóstico de leucemia linfoblástica aguda de alto riesgo, de múltiples transfusiones de glóbulos rojos sin tratamiento radiante previo, a quien se le realiza el diagnóstico dermatológico e histopatológico de EICH aguda postransfusional.

Graft vs host disease (GVHD) is a process triggered by immune T cells, that react immunocompetent donor tissue against a recipient immunocompromised. This causes severe multiorgan damage in the receiving agent. Cutaneous manifestations are an important clinical sign in early periods. Postranfusional GVHD (GVHD-PT) is a rare entity with high mortality rate, observed in patients after transfusion with blood products not previously irradiated. Corticosteroids are the mainstay of treatment after installation of the box, however prevention with leukoreduction and irradiation of blood components remains the mainstay to prevent its development. We present a patient 5 years of age, diagnosed with acute lymphoblastic leukemia at high risk with a history of multiple transfusions of red blood cells without prior radiation treatment, who is performed dermatological and histopathological diagnosis posttransfusion acute GVHD.

Se describió el comportamiento de la leptospirosis grave en 35 pacientes ingresados en la Unidad de Cuidados Intensivos de Adultos del Instituto de Medicina Militar "Dr. Luis Díaz Soto." Se recogieron los datos de las historias clínicas de los afectados con este diagnóstico comprobado por pruebas serológicas. Se realizó análisis univariado para cada variable mediante la prueba de la t de Student y de Chi cuadrado. Se usó el método de regresión logística para determinar factores asociados a la mortalidad. El 20 % de las personas con leptospirosis grave falleció, no se halló relación significativa de la tenencia de animales o vivir en zona rural con respecto a la mortalidad. Se comprobó el 71,4 % de errores diagnósticos en el grupo de fallecidos, el principal fue la sepsis urinaria. Los factores pronósticos de mayor relación con la mortalidad (p < 0,05; 95 % de confianza) fueron: a) días de inicio de los síntomas, b) estancia en la UCIA, c) días de ventilación mecánica, d) relación PO2/FiO2, f) valor del APACHE II, g) requerimiento de fármacos inotrópicos, h) presencia de insuficiencia renal aguda, i) existencia de coagulopatías, j) exigencia de transfusiones de glóbulos, k) necesidad de depuración extrarrenal, l) manifestaciones de disfunción múltiple de órganos. Se concluyó que la leptospirosis se comportó como una urgencia médica y precisa de una adecuada atención por parte de los servicios de atención al grave.

The behavior of severe leptospirosis in 35 patients admitted at the Adults' Intensive Care Unit of "Dr. Luis Díaz Soto" Institute of Military Medicine was described. Data were taken from the medical records of those patients, whose diagnosis had been confirmed by serological tests. Univariate analysis was made for each variable by Student's t test and Chi square test. The logistical regression method was used to determine factors associated with mortality. 20 % of the persons with severe leptospirosis died .No significant relation was found between having animals, or living in a rural area and mortality. 71.4 % of the diagnositc errors in the group of deaths were proved. Urinary sepsis was the main of them. The prognostic factors showing the highest relation with mortality (p < 0.05; 95 % of confidence) were: a) days of onset of the symptoms, b) stay at the AICU, c) ratio PO2/FiO2, f) APACHE II value, g) requirement of inotropic drugs, h) presence of acute renal failure. i) existance of coagulopathies, j) demand of globules transfusions, k) need of extrarenal depuration, l) manifestations of multiple organ dysfunction. It was concluded that leptospirosis behaved as a medical emergency and that the severe patient care service should give it an appropriate attention.