Abstract Objective: To evaluate the conservative management of newborns born at ≥35 weeks of gestational age, at risk for early-onset neonatal sepsis (EOS). Methods: Retrospective, analytic cohort study (2016 to 2019), including newborns ≥35 weeks of gestational at risk of EOS, asymptomatic at birth, managed conservatively in full rooming-in: serial physical examination and clinical observation for at least 48 h. They were classified into three groups, according to the clinical course: asymptomatic (group A), symptomatic for other reasons (group B), and with sepsis (group C). Risk factors, clinical signs and differential diagnoses of sepsis, length of stay, and discharge conditions were evaluated. Results: The authors evaluated 769 asymptomatic newborns at risk of EOS. (mean birth weight 2999 ± 485 g and gestational age 37.6 ± 1.7 weeks, respectively) corresponding to 12.2% of rooming-in admissions. The most prevalent risk factors were colonization by Group B Streptococcus (29%), prolonged rupture membrane duration (21.9%) and preterm labor (21.4%). Most of all of them (53.9%) remained asymptomatic (group A). Group B corresponded for 45.3%, and the most common clinical signs were hypothermia (24.5%), tremors (8.7%) and vomiting (8%). Environmental dysthermia (50.7%), prematurity (20.0%), and feeding intolerance (15.7%) were common in Group B. Laboratory tests were performed in 3.5%. Five patients (one confirmed) comprised group C (0.8/1,000 live births). There were no deaths. The median length of stay was 64 h (IQR 50-93). Conclusion: The rate of clinical/confirmed EOS was low. Most of the symptomatic patients only needed clinical evaluation to rule out sepsis. Management was shown to be safe.

Abstract Different degrees in the biological activities of Canavalia rosea had been previously reported . In this study, our group assessed the cardioprotective effects of the ethyl acetate fraction (EAcF) of the Canavalia rosea leaves. Firstly, it was confirmed, by in vitro approach, that the EAcF has high antioxidant properties due to the presence of important secondary metabolites, as flavonoids. In order to explore their potential protector against cardiovascular disorders, hearts were previously perfused with EAcF (300 μg.mL-1) and submitted to the global ischemia followed by reperfusion in Langendorff system. The present findings have demonstrated that EAcF restored the left ventricular developed pressure and decreased the arrhythmias severity index. Furthermore, EAcF significantly increased the glutathiones peroxidase activity with decreased malondialdehyde and creatine kinase levels. EAcF was effective upon neither the superoxide dismutase, glutationes reductase nor the catalase activities. In addition, the Western blot analysis revealed that ischemia-reperfusion injury significantly upregulates caspase 3 protein expression, while EAcF abolishes this effect. These results provide evidence that the EAcF reestablishes the cardiac contractility and prevents arrhythmias; it is suggested that EAcF could be used to reduce injury caused by cardiac reperfusion. However more clinical studies should be performed, before applying it in the clinic.

ABSTRACT Leishmania infantum is a protozoan that causes visceral leishmaniasis (VL) in the Americas and some regions of Europe. The disease is mainly characterized by hepatosplenomegaly and fever, and can be fatal. Factors related to the host and parasite can contribute to the transmission of Leishmania and the clinical outcome. The intraspecific genetic variability of L. infantum strains may be one of these factors. In this study, we evaluated the genetic variability of L. infantum obtained from bone marrow smear slides from patients in the Sao Paulo State, Brazil. For this, the minicircle of the kDNA hypervariable region was used as target by Sanger sequencing. By analyzing the similarity of the nucleotides and the maximum likelihood tree (Fasttree), we observed a high similarity (98%) among samples. Moreover, we identified four different profiles of L. infantum. In conclusion, L. infantum strains from Sao Paulo State, Brazil, showed low diversity measured by minicircle of the kDNA hypervariable region.

ABSTRACT In order to establish minimum criteria related to social innovation in agricultural community organizations, qualitative research tools were used, including specialized searches and the use of programs for the analysis of bibliographic data (for example VOSviewer™), with the purpose of defining the elements related to management, governance, capacities, business model and sustainability for eight (8) community organizations of agricultural producers in the province of Sugamuxi, in the department of Boyacá, Colombia. It was possible to establish that some organizations of agricultural producers that carry out activities in the department of Boyacá are not integrally appropriating the elements of social innovation (at operational and managerial levels) and, consequently, there is a whole route of improvement to be developed with them to optimize the organizational efficiency indexes; in addition, the complexity of the subject is evident, given that the sustainability of social innovations will depend largely on the management of the organizations, where shared institutional agendas contextualized and adjusted to the territories are established. It is necessary to optimize the channels of participation in a joint manner between the State, the organizations, and the producers themselves to be able to dynamize processes, goals and the real scope of social innovation in light of the accelerated changes faced by the agribusiness producer in Colombia, related to market dynamics, access to resources and others.

RESUMEN Con el objetivo de establecer criterios mínimos relativos a la innovación social en organizaciones comunitarias agropecuarias, se hizo uso de herramientas propias de la investigación cualitativa que incluyeron búsquedas especializadas y uso de programas para análisis de datos bibliográficos (por ejemplo VOSviewer™) para determinar los elementos relativos a la gestión, gobernanza, capacidades, modelo de negocio sostenibilidad para ocho (8) organizaciones comunitarias de productores agropecuarios de la provincia del Sugamuxi, en el departamento de Boyacá, Colombia. Se pudo establecer que algunas organizaciones de productores agropecuarios que ejercen actividades en el departamento de Boyacá no están apropiando de forma integral los elementos de innovación social (en niveles operativos y gerenciales) y, consecuentemente, existe toda una ruta de mejoramiento por desarrollar con estas para optimizar los índices de eficiencia organizacional; además, se evidencia la complejidad de la temática, dado que la sostenibilidad de las innovaciones sociales dependerá en gran medida de la gestión de las organizaciones, donde se establezcan agendas institucionales compartidas contextualizadas y ajustadas a los territorios. Es necesario optimizar los canales de participación de manera mancomunada entre el Estado, las organizaciones y los mismos productores para poder dinamizar procesos, metas y alcances reales de la innovación social a la luz de los acelerados cambios a los que se enfrenta el productor agropecuario agremiado en Colombia, relativos estos a la dinámica del mercado, el acceso a recursos y otros.

There is a trend of development of analytical methodologies and technologies that allow in situ analysis, producing accurate information in real time, at low cost, using homemade experiments and devices to increase interest in scientific knowledge with a constructive approach in an interdisciplinary perspective in Chemistry Education. In this context, a photometric-chemometric method of analysis was developed to measure the pH of solutions using easily accessible and low-cost material based on the use of natural pigments found in red cabbage (Brassica oleracea L.). Calibrations and determinations were performed by RGB measurements of pigment coloration in solution at different pH values using the free app Photometrix, converted to HSV, YCbCr and YUV color spaces and processed by Partial Least Squares regression (PLS). The best PLS model found was HSV with mean central scale obtained pH measurements with RMSEP 0.98, R2 0.97 and bias close to zero. In addition, experimental data were statistically validated. Analyzes of predicted pH from three independents experiments revealed high recoveries (95-103%) and low relative standard deviations. Thus, the PhotoMetrix app was viable for colorimetric pH determinations using the low-cost photometric pH meter sensor and a smartphone, improving accessibility and applicability in Chemistry Education.

ABSTRACT A mathematical model was developed to describe the dynamics of the primary infection of dengue virus in infants who were born to a mother immune to some serotype of the dengue virus. The model is given by a system of nonlinear ordinary differential equations with time-dependent variables for the number of dengue virus antibodies of the infant transferred from their immune, uninfected, and infected monocytes and dengue virus. The mathematical analysis was carried out where the conditions for the existence of the disease-free equilibrium and the endemic equilibrium were established. The numerical simulations were performed considering different scenarios for a basic reproductive number, R 0, illustrating the global convergence of the numerical results for the equilibrium points. The results are in agreement with our derived global stability analysis. It can be concluded that dengue hemorrhagic fever in infants could occur in the peaks observed for the infected monocytes and dengue virus.

Abstract Objectives: To evaluate the mechanical, physicochemical, and antimicrobial properties of four different formulations containing micro- or nanoparticles of sodium trimetaphosphate (mTMP and nTMP, respectively). Methodology: Four experimental groups were used in this investigation: two mTMP groups and two nTMP groups, each containing zirconium oxide (ZrO2), and solution containing either chitosan or titanium oxide (TiO2) nanoparticles (NPs). Setting time, compression resistance, and radiopacity were estimated. The agar diffusion test was used to assess the antimicrobial activity of the formulations against five different microbial strains: Streptococcus mutans, Lactobacillus casei, Actinomyces israelii, Candida albicans, and Enterococcus faecalis. Parametric and nonparametric tests were performed after evaluating homoscedasticity data (p<0.05). Results: From the properties evaluated, nTMP cements required less setting time and showed greater resistance to compression. Cements containing TiO2 showed greater radiopacity for both nTMP and mTMP. All four cement formulations showed antimicrobial activity against S. mutans and L. casei Conclusion: Formulations containing nTMP have shorter setting times and higher compressive strength, and those with TiO2 nanoparticles showed antimicrobial activities. Clinical relevance: The cement containing nTMP, ZrO2, and TiO2 could be an alternative material for protecting the pulp complex.

Resumo A vitamina D desempenha um papel importante na função imunológica e na inflamação e a atividade física demonstra relação próxima à síntese de vitamina D, sendo necessária a fatores imunológicos, ambientais e físicos. Pesquisamos cinco bancos de dados até 20 de fevereiro de 2021. Dois revisores examinaram os estudos, coletaram dados, avaliaram o risco de viés e classificaram as evidências para cada resultado nos estudos, de forma independente e em duplicata. Os endpoints de interesse pré-especificados foram aptidão cardiorrespiratória; Exposição à luz solar; Índice de Massa Corporal (IMC). Incluímos apenas dados de artigos revisados por pares em nossas análises primárias. Em nossa análise primária, houve uma tendência positiva entre 25(OH)D sérico <20 ng/ml e índice de massa corporal., esse resultado deve ser interpretado com cautela, considerando os intervalos de confiança (RR 1,10 IC 95% 0,37 a 1,83. Identificamos 4 evidências de alta qualidade de que níveis de vitamina D e atividade física alta exigiam uma relação direta considerando (quatro ensaios com 2.253) ; RR 0 . (RR 0,0; IC 95% -0,15 a 0,15) (RR 0,59; IC 95% 0,43 a 0,75. Embora as evidências disponíveis até agora, de estudos observacionais de qualidade média, possam ser vistas como uma tendência de associação entre níveis séricos suficientes de 25(OH)D e atividade física, essa relação foi demonstrada. têm um efeito estimulante na síntese de vitamina D, a relação de baixo índice de massa corporal com níveis suficientes de vitamina D não é baseada em evidências sólidas. Aguardamos resultados de estudos em andamento para determinar essa eficácia.

Abstract Vitamin D plays an important role in immune function and inflammation and the physical activity demonstrate relationship near to syntheses of vitamin D, considering to necessary to factors immunologics, environmental and physical. We searched five databases through February 20, 2021. Two reviewers screened the studies, collected data, assessed the risk of bias, and ranked the evidence for each outcome across the studies, independently and in duplicate. The prespecified endpoints of interest were Cardiorespiratory Fitness; Sunlight Exposure; Body Mass Index (BMI). We only included data from peer-reviewed articles in our primary analyses. In our primary analysis, there was a positive trend between serum 25(OH)D <20 ng/ml and body mass index, this result should be interpreted with caution, considering confidence intervals (RR 1.10 95% CI 0.37 to 1.83. We identified 4 high quality evidence that vitamin D levels and high physical activity required a direct relationship considering (four trials with 2,253) ; RR 0. (RR 0.0; 95% CI -0.15 to 0.15) (RR 0.59; 95% CI 0.43 to 0.75. Although the evidence available so far, from observational studies of medium quality, can be seen as showing a trend towards an association between sufficient serum levels of 25(OH)D and physical activity, this relationship has been shown. have a stimulating effect on vitamin D synthesis, the relationship of low body mass index with sufficient vitamin D levels is not based on solid evidence. We await results from ongoing studies to determine this effectiveness.

RESUMO Objetivou-se neste estudo quantificar o efeito de variações da temperatura da água na vazão de fitas gotejadoras colapsáveis, de parede fina, com emissores integrados não regulados. Os ensaios foram conduzidos em laboratório utilizando estrutura de ensaios automatizada. Foram realizados ensaios para determinação das curvas vazão-pressão sob temperaturas de ensaio na faixa de 20 a 50 ºC, para nove modelos de emissores com três espessuras de parede (6, 8 e 9 MIL). Curvas vazão-pressão apresentaram distintos parâmetros K e x para cada temperatura. Para os emissores planos de fluxo turbulento (x < 0,5), a vazão diminuiu com o incremento da temperatura. Para os emissores contínuos de fluxo turbulento, respostas diferentes foram obtidas, sendo que no emissor D (x > 0,5) a vazão aumentou com o incremento da temperatura, enquanto no emissor C (x < 0,5), a vazão diminuiu e as maiores variações de vazão ocorreram a partir de 60 kPa. Para o emissor moldado, a vazão aumentou em função da temperatura, porém, a maior variação ocorreu nas menores pressões. Para nenhum dos emissores houve diferença significativa na variação de vazão entre as espessuras de parede.

ABSTRACT The objective of this study was to quantify the effect of water temperature variations on the discharge of collapsible thin-walled drip tapes with integrated non-pressure-compensating emitters. The tests were conducted in the laboratory using an automated test bench. Tests were performed to determine the discharge-pressure curves by varying the water temperature from 20 to 50 °C. Nine emitter models of three wall thicknesses (6, 8, and 9 MIL) were evaluated. The coefficients K and x of the discharge-pressure curves varied according to the water temperature. In flat emitters of turbulent flow (x < 0.5), the discharge decreased as the temperature increased. In the welded emitters of turbulent flow, several responses were observed. Regarding emitter D (x > 0.5), the discharge increased as the temperature was increased, while for emitter C (x < 0.5), the discharge decreased; the highest discharge variations occurred at pressures higher than 60 kPa. For embossed emitters, the discharge increased as a function of temperature, however, the greatest variation occurred at the lowest pressures. None of the emitters showed significant difference in the discharge variation due to wall thicknesses.

Abstract Candida spp. are naturally opportunistic and can promote infections. These yeasts can form biofilm, after penetration and adhesion to the biotic or abiotic surfaces. Preexisting diseases, treatments with drugs and radiation therapy, medical procedures, and parafunctional habits favor the installation of a fungal infection. Increased resistance to the available antifungals has become a concern. Therefore, alternative methods to control them have been evaluated, including the use of plant substances. In this study, the antibiofilm effect of R. officinalis L. extract was analyzed on C. albicans, C. dubliniensis, C. glabrata, C. krusei, and C. tropicalis. A phytochemical analysis of the extract was performed. Biofilms were formed for 48 h and exposed to the different concentrations of the extract (50, 100, and 200 mg/mL) for 5 min or 24 h. The effect of the plant extract was compared to the antifungal nystatin. Rosmarinus officinalis L. extract was constituted of phenols and flavonoids, highlighting the presence of chlorogenic acid derivatives in its composition. Biofilm reductions were observed after exposure to the plant extract for both periods. The plant extract provided a reduction similar to the antifungal. Thus, R. officinalis L. extract showed antibiofilm effect on Candida spp. comparable to the nystatin.

ABSTRACT Objective: Obesity is characterized by a state of chronic, low-intensity systemic inflammation frequently associated with insulin resistance and dyslipidemia. Materials and methods: Given that chronic inflammation has been implicated in the pathogenesis of mood disorders, we investigated if chronic obesity that was initiated early in life – lasting through adulthood – could be more harmful to memory impairment and mood fluctuations such as depression. Results: Here we show that pre-pubertal male rats (30 days old) treated with a high-fat diet (40%) for 8-months gained ~50% more weight when compared to controls, exhibited depression and anxiety-like behaviors but no memory impairment. The prefrontal cortex of the obese rats exhibited an increase in the expression of genes related to inflammatory response, such as NFKb, MMP9, CCl2, PPARb, and PPARg. There were no alterations in genes known to be related to depression. Conclusion: Long-lasting obesity with onset in prepuberal age led to depression and neuroinflammation but not to memory impairment.

RESUMO O objetivo deste estudo foi avaliar o teor residual da aplicação de doses fosforo (P) via fertilizante organomineral (FO) em dois solos cultivados com repolho e alface em sucessão. Em blocos casualizados, utilizou-se cinco doses de FO como fonte de P. No Oxisol aplicaram-se: 0; 50; 100; 200 e 300% da dose de P recomendada para o repolho com utilização de FO, mais um tratamento com 100% da dose via fertilizante mineral. No Entisol aplicaram-se: 0; 50; 100; 200 e 300% da dose de P com FO para a cultura do repolho, além de um tratamento adicional (100% mineral), com quatro repetições. Avaliaram-se a produção de massa fresca (MF) e seca (MS), estado nutricional do repolho e da alface e o efeito residual de nutrientes no solo. O teor residual do P no solo aumentou (p ≤ 0,05) quanto maior foi à dose de FO utilizada no Oxisol nos dois ciclos avaliados, enquanto que no Entisol isto só ocorreu no primeiro ciclo. Usando 50% da dose de FO no repolho em ambos os solos proporciona produção de MF e MS no mínimo 11% superior, comparado à adubação mineral.

ABSTRACT The objective of this study was to evaluate the residual concentration from the application of levels of phosphorus contained in an organomineral fertilizer (OMF) in two soils cultivated with cabbage and lettuce in succession. In randomized blocks, five doses of OMF were used as a source of P in Oxisol (0, 50, 100, 200, and 300% of the P dose was recommended for cabbage crop using OMF), plus an additional treatment (100% mineral). The doses applied to the Entisol were as follows: 0, 50, 100, 200, and 300% of the recommended P dose with OMF for cabbage crop, plus an additional treatment (100% mineral), with four repetitions. The production of fresh matter (FM) and dry matter (DM), the nutritional status of the cabbage and lettuce, and the residual effect of nutrients in the soil were also evaluated. The P concentration in the soil increased (p ≤ 0.05) with higher doses of OMF in the Oxisol in the two cycles evaluated. However, this occurred only in the first cycle in the Entisol. Using the 50% dose of OMF in the cabbage in both soils provided FM and DM production values that were at least 11% higher than those attained with mineral fertilizer.

RESUMO O conforto térmico no interior de instalações de criação de frangos de corte é essencial na obtenção de bons resultados nesta atividade de produção. A avaliação das condições termodinâmicas adequadas requer a medição e controle, geralmente implicando em custos e manutenção especializada. Neste trabalho, objetivou-se monitorar a distribuição de temperatura, umidade relativa e velocidade do ar utilizando sistema desenvolvido, de baixo custo, de código aberto e de fácil uso, com Arduíno (hardware) e Scilab (software) para aquisição de dados em tempo real. Os sensores foram instalados em uma unidade de produção existente (Cianorte, PR), com medições para o ambiente interno (20 sensores de temperatura/umidade e 2 sensores de pressão diferencial, respectivamente, a 0,5 e 1,5 m de altura) e para ambiente externo (pressão e velocidade do vento, a 1,5 m de altura). Sistema de aquisição de dados permitiu a comunicação com sensores, que são facilmente lidos por computador e armazenados em um arquivo de dados. O sistema de aquisição de dados desenvolvido provou-se eficiente quando aplicado em uma instalação de criação comercial de frangos de corte, permitindo o monitoramento em tempo real para os parâmetros de conforto térmico.

ABSTRACT Thermal comfort inside broiler husbandry facilities is essential in obtaining good results in the production activity. Assessment of adequate thermodynamic conditions requires measurement and control, usually implying costs and specialized maintenance. The objective of this research was to monitor the temperature, relative humidity and air speed distributions by a developed low-cost, open-source and easy-to-use measurement system, using Arduino (hardware) and Scilab (software) for real-time data acquisition. Sensors were installed in a real facility (Cianorte, PR), with measurements for internal ambient (20 sensors for temperature/relative humidity, and two sensors for differential pressure, respectively 0.5 and 1.5 m high) and external ambient (pressure and wind speed, 1.5 m high). Data acquisition system has enabled communication with sensors which are easily read by the computer and stored in a data file. The developed data acquisition system proved to be efficient when applied in a commercial broiler husbandry facility, enabling real-time monitoring for thermal comfort parameters.

ABSTRACT This is the first report of Cotesia scotti (Valerio and Whitfield) comb. nov. in Brazil, attacking larvae of the black armyworm, Spodoptera cosmioides, and the southern armyworm, S. eridania. The moth larvae were found respectively, infesting a protected cropping of organic tomato in Hidrolândia, Goiás, Brazil, and a transgenic soybean crop in São José dos Pinhais, Paraná, Brazil. Biological, molecular and morphological characters were used to confirm the identity of the specimens. Parasitoid identification presented a challenge since the species has most diagnostic characters of the genus Cotesia Cameron, but few in the poorly defined genus Parapanteles Ashmead. Based on morphological and molecular evidence, we transfer Parapanteles scotti to the genus Cotesia. The new combination is discussed by comparison with morphologically similar species and available molecular data.

Resumo O controle higiênico e sanitário nas Unidades de Alimentação e Nutrição (UAN) é considerado um procedimento padrão para produzir refeições adequadas e reduzir o risco de doenças transmitidas pelos alimentos e infecções hospitalares. Este estudo teve como objetivo isolar e identificar bactérias de equipamentos e superfícies de contato com alimentos em uma UAN hospitalar, bem como avaliar a condição sanitária. Do mesmo modo, analisou-se a adesão dos micro-organismos em tábuas de corte de polietileno. A presença de micro-organismos aeróbios mesófilos, leveduras, fungos, Sthapylococcus coagulase-positivos, coliformes, coliformes fecais e Escherichia coli foi analisadas na superfície de mesas do refeitório, superfícies de bancada e tábuas de corte usadas para manuseio de carne ou vegetais e, em equipamentos como micro-ondas e refrigeradores. A identificação molecular foi feita pelo sequenciamento do gene 16S rRNA. A adesão dos micro-organismos (formação de biofilmes) em tábuas de corte de carne e de vegetais também foi avaliada por microscopia eletrônica de varredura. Os resultados mostraram elevada contagem para todos os micro-organismos analisados, exceto para E. coli, a qual não foi observada nas amostras. A análise molecular identificou espécies da família Enterobacteriaceae e Pseudomonadaceae. A análise de microscopia eletrônica de varredura revelaram adesão bacteriana nas superfícies das placsa de corte. Os resultados obtidos neste estudo indicaram que as condições higiênicas das superfícies e de equipamentos nesta UAN hospitalar estavam inadequadas. A aplicação de procedimentos operacionais padrão poderia auxiliar positivamente na padronização do controle higiênico-sanitário, reduzindo a contaminação microbiana e fornecendo um alimento seguro para pacientes hospitalizados.

Abstract The hygienic and sanitary control in Food and Nutrition Units (FNU) is considered a standard procedure to produce adequate meals and reduce the risk of foodborne diseases and hospital infections. This study aimed to evaluate the isolation and identification of bacteria from equipment and food contact surfaces in a hospital FNU as well as to evaluate the sanitary condition. Likewise, it was analyzed the adhesion of the microorganisms on polyethylene cutting boards. The presence of aerobic mesophilic microorganisms, yeasts, molds, coagulase-positive staphylococci, coliform and fecal coliform, and Escherichia coli were analyzed on eating tables, countertop surfaces and cutting boards used for meat or vegetable handling, and equipment such as microwaves and refrigerators. The molecular identification it was done by 16S rRNA gene sequencing. The adhesion of the microorganisms (biofilm formation) on meat and vegetable cutting boards was also evaluated by scanning electron microscopy. The results showed high numbers of all microorganisms, except for E. coli , which was not observed in the samples. The molecular analysis identified species of the Enterobacteriaceae family and species of the Pseudomonadaceae family. Scanning electron microscopy analyses revealed bacterial adhesion on the cutting board surfaces. The results obtained in this study indicated that the hygienic conditions of surfaces like plastic cutting boards and equipment in this hospital FNU were inadequate. The achievement and application of standard operating procedures could positively help in the standardization of sanitary control, reducing the microbial contamination and providing a safe food to hospitalized patients.