SUMMARY OBJECTIVE: This study aimed to evaluate the expression of C-X-C motif chemokine ligand 12 and its C-X-C chemokine receptor type 4, and the tumor-stroma ratio using collagen stromal content of breast cancer samples, correlating it with clinicopathological data. METHODS: Through a retrospective cohort study, samples were obtained from female patients, over 18 years of age, with the disease in stages 1–4, who underwent mastectomy or lumpectomy. The biopsies were provided by the Oncology sector of the Hospital das Clínicas of Universidade Federal de Pernambuco, Recife city, in 2011–2014, including samples of invasive ductal carcinoma, ductal carcinoma in situ, or benign changes (fibroadenoma and hypertrophy), which were analyzed between 2020 and 2022 by immunohistochemistry for the expression of stromal cell characteristics. Collagen content was tested by Gomori staining and digital analysis of images. RESULTS: Absence of stromal expression of C-X-C motif chemokine ligand 12 was associated with longer disease-free survival (disease-free survival=0.481), and expression of C-X-C chemokine receptor type 4 was associated with lower disease-free survival. An association was observed between clinicopathological variables and stromal expression of chemokines, that is, an association of stromal C-X-C motif chemokine ligand 12 with histological grade, angiolymphatic invasion, and an association between C-X-C chemokine receptor type 4 expression and histological grade. Analyses of digital pixels images of collagen and tumor cells showed a lower percentage of collagen in the invasive ductal carcinoma samples (39%), unlike samples without neoplasms (78%). CONCLUSION: Low expression of C-X-C motif chemokine ligand 12 may be associated with a worse prognosis for breast cancer. Collagen staining analyzed using digital images represents an opportunity for clinical application and is indicative of the prognosis of the tumor microenvironment in breast carcinoma. OBJECTIVE CXC C X 1 tumorstroma stroma data METHODS patients age 14 1–4 lumpectomy Pernambuco city 20112014 2011 2014 2011–2014 situ fibroadenoma hypertrophy, hypertrophy , hypertrophy) 202 characteristics RESULTS diseasefree free survival=0.481, survival0481 survival=0.481 0 481 survival=0.481) chemokines grade invasion 39%, 39 39% (39%) 78%. 78 78% . (78%) CONCLUSION 1– 2011201 201 2011–201 20 survival048 survival=0.48 48 3 (39% 7 (78% 201120 2011–20 2 survival04 survival=0.4 (39 (78 20112 2011–2 survival0 survival=0. (3 (7 2011– survival=0 ( survival=