Abstract Fermentation using starter cultures has been considered an alternative and economically viable technology for the production of specialty coffees. This type of technology promotes several benefits, such as increased sensory quality, control over the fermentation process, predictability of the final product and added value. Coffee (Coffea arabica L.) samples for this study were collected in Presidente Olegário - MG (2018/19 crop year) in the Cerrado region of Minas Gerais. The effects of natural fermentation and inoculation of the yeast Torulaspora delbrueckii and duration of fermentation (0, 24, 48, 72 and 96 hours) on the sensory and chemical quality (analysis of bioactive, volatile, and organic compounds and fatty acids) of coffee were evaluated. The objective of this study was to determine the effect of fermentation time and starter culture inoculation on the chemical composition of fermented coffees. Fermentation time significantly influenced the sensory description of the coffee beverage, with notes of honey, brown sugar and almond predominating up to 48 hours, for coffees fermented for 72 and 96 hours the notes described were and fruity, winey notes. The chemical composition was primarily influenced by fermentation time. benefits process value Coffea L. L 2018/19 201819 2018 19 (2018/1 year Gerais 0, 0 (0 24 7 9 analysis bioactive volatile acids evaluated beverage honey 4 fruity 2018/1 20181 201 1 (2018/ ( 2 2018/ 20 (2018 (201 (20 (2

ABSTRACT Numerous factors are related to the individual sensory perception of consumers, which makes it impossible to adapt a model that explains their behavior. In this context and given the scarcity of statistical indexes that evaluate preferences for specialty coffees, new statistical methods should be studied. To this end, our study aimed to create an index that measures the acceptance of specialty coffees. The index was built considering the fit of regression models as a function of principal component scores. Validation was done by significance tests, whose probabilities were obtained by bootstrapping, considering the main measures used in diagnosing outliers as weights, with application to real data from different consumer groups. The coffee varieties Acaia and Bourbon were discriminated in relation to altitude. In conclusion, the index was adequate for the analysis and characterization of specialty coffees grown at different altitudes.

RESUMO Objetivou-se utilizar a metodologia de resposta simultânea aplicada a um delineamento experimental para determinar a melhor combinação de tempo e das condições de armazenamento para a conservação de grãos de café. Frutos de Coffea arabica L. foram colhidos no estádio de maturação cereja, processados por via úmida ou por via seca e secados até atingir 11% de teor de água. Parte dos grãos foi beneficiada e parte não foi beneficiada antes de ser armazenada em duas condições de ambiente: em ar refrigerado a 10 ºC e umidade relativa de 50% e em 25 ºC sem controle da umidade relativa do ar. Nos períodos de 3, 6 e 12 meses, foram retiradas amostras para avaliação da qualidade. Os dados foram submetidos à técnica de otimização de resposta simultânea para cada processamento e beneficiamento separadamente, em delineamento inteiramente casualizado e esquema fatorial 2 x 3 (duas condições de armazenamento e três períodos de armazenamento). Conclui-se que a utilização da metodologia de resposta simultânea é viável para ser aplicada para determinação das condições ideais de armazenamento em condição refrigerada.

ABSTRACT The objective of the study was to use methodology of simultaneous optimization of multiple responses applied to an experimental design to determine the best combination of storage period and conditions for preservation of coffee beans. Coffea arabica L. fruits were harvested in the ripe stage of maturation, processed using wet and dry methods, and dried to 11% (wet basis) moisture content. Part of the beans was hulled, while the other part was hulled only after the beans were stored under two different environmental conditions: cooled air at 10 ºC with 50% relative humidity; and at 25 ºC without controlling the relative humidity. Samples were taken at 3, 6, and 12 months intervals in order to evaluate quality. The data were submitted to the simultaneous optimization of responses for each processing and hulling condition separately, in a completely randomized design and 2 x 3 factorial scheme (two storage conditions and three storage periods). In conclusion, the use of the simultaneous optimization of responses is viable to be applied for determining the ideal storage conditions in a refrigerated condition.

The genera Aspergillus comprises species that produce mycotoxins such as aflatoxins, ochratoxins and patulin. These are cosmopolitan species, natural contaminants of agricultural products. In coffee grains, the most important Aspergillus species in terms of the risk of presenting mycotoxins belong to the genera Aspergillus Section Circumdati and Section Nigri. The purpose of this study was to assess the occurrence of isolated ochratoxigenic fungi of coffee grains from organic and conventional cultivation from the South of Minas Gerais, Brazil, as well as to evaluate which farming system presents higher contamination risk by ochratoxin A (OTA) produced by fungi. Thirty samples of coffee grains (Coffea arabica L.) were analysed, being 20 of them of conventional coffee grains and 10 of them organic. The microbiological analysis was done with the Direct Plating Technique in a Dichloran Rose Bengal Chloramphenicol Agar (DRBC) media. The identification was done based on the macro and micro morphological characteristics and on the toxigenic potential with the Plug Agar technique. From the 30 samples analysed, 480 filamentous fungi of the genera Aspergillus of the Circumdati and Nigri Sections were isolated. The ochratoxigenic species identified were: Aspergillus auricoumus, A. ochraceus, A. ostianus, A. niger and A. niger Aggregate. The most frequent species which produces ochratoxin A among the isolated ones was A. ochraceus, corresponding to 89.55%. There was no significant difference regarding the presence of ochratoxigenic A. ochreceus between the conventional and organic cultivation systems, which suggests that the contamination risk is similar for both cultivation systems.