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Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
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SOUZA-SILVA, GUILHERME ANTONIO DE

; AQUINO, RODRIGO CESAR A. DE

; SOUSA, GEORON F. DE

; BARROS, BÁRBARA RAFAELA S.

; LIMA, MARÍLLIA RAPHAELLA C.F. DE

; CRUZ, LEONARDO C.O.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; TALES, APARECIDA VIRGÍNIA S.

; DINIZ, MADI V.

; LIMA, MÔNICA MARIA C.

; SÁ, IGOR W.A. DE

; RODRIGUES, FÁBIO AUGUSTO C.

; SOUZA, EDSON B. DE

; NASCIMENTO, ARIONE V. DO

; VIEIRA, AMANDA M.

; CARVALHO, BRUNO M. DE

; CAMPELO JÚNIOR, EVÔNIO B.

; FREITAS, ANTONIO CARLOS DE

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 3 elocation e20211590

Resumo: En | Texto: En | PDF: En

Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

https://doi.org/10.1590/0001-3765202220211590

Clinical and epidemiological aspects of Candidemia in eight medical centers in the state of Parana, Brazil: Parana Candidemia Network
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Oliveira, Carla Sakuma de

; Colombo, Arnaldo Lopes

; Francisco, Elaine Cristina

; Lima, Bernardo de

; Gandra, Rinaldo F.

; Carvalho, Mariza Cristina Preifz de

; Carrilho, Cláudia Maria Dantas de Maia

; Petinelli, Renan

; Pelison, Marsilene

; Helbel, Cesar

; Czelusniak, Gerson

; Paz Morales, Hugo Manuel

; Perozin, Jamile Sardi

; Pinheiro, Rosangela Lameira

; Cognialli, Regielly

; Breda, Giovanni Luis

; Queiroz-Telles, Flávio

Brazilian Journal of Infectious Diseases

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2021, Volume 25 Nº 1 elocation 101041

Resumo: En | Texto: En | PDF: En

ABSTRACT Objectives: Candida spp. has been reported as one of the common agents of nosocomial bloodstream infections and is associated with a high mortality. Therefore, this study evaluated the clinical findings, local epidemiology, and microbiological aspects of candidemia in eight tertiary medical centers in the state of Parana, South of Brazil. Methods: In this study, we reported 100 episodes of candidemia in patients admitted to eight different hospitals in five cities of the state of Parana, Brazil, using data collected locally (2016 and 2017) and tabulated online. Results: The incidence was found to be 2.7 / 1000 patients / day and 1.2 / 1000 admissions. C. albicans was responsible for 49% of all candidemia episodes. Cancer and surgery were the two most common underlying conditions associated with candidemia. The mortality rate within 30 days was 48%, and removal of the central venous catheter (p = 0.029) as well as empirical or prophylactic exposure to antifungals were both related to improved survival (p = 0.033). Conclusions: This study highlights the high burden and mortality rates of candidemia in hospitals from Parana as well as the need to enhance antifungal stewardship program in the enrolled medical centers.

https://doi.org/10.1016/j.bjid.2020.11.006 198 downloads

Diretriz Brasileira de Cardio-oncologia – 2020
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Hajjar, Ludhmila Abrahão

; Costa, Isabela Bispo Santos da Silva da

; Lopes, Marcelo Antônio Cartaxo Queiroga

; Hoff, Paulo Marcelo Gehm

; Diz, Maria Del Pilar Estevez

; Fonseca, Silvia Moulin Ribeiro

; Bittar, Cristina Salvadori

; Rehder, Marília Harumi Higuchi dos Santos

; Rizk, Stephanie Itala

; Almeida, Dirceu Rodrigues

; Fernandes, Gustavo dos Santos

; Beck-da-Silva, Luís

; Campos, Carlos Augusto Homem de Magalhães

; Montera, Marcelo Westerlund

; Alves, Sílvia Marinho Martins

; Fukushima, Júlia Tizue

; Santos, Maria Verônica Câmara dos

; Negrão, Carlos Eduardo

; Silva, Thiago Liguori Feliciano da

; Ferreira, Silvia Moreira Ayub

; Malachias, Marcus Vinicius Bolivar

; Moreira, Maria da Consolação Vieira

; Valente Neto, Manuel Maria Ramos

; Fonseca, Veronica Cristina Quiroga

; Soeiro, Maria Carolina Feres de Almeida

; Alves, Juliana Barbosa Sobral

; Silva, Carolina Maria Pinto Domingues Carvalho

; Sbano, João

; Pavanello, Ricardo

; Pinto, Ibraim Masciarelli F.

; Simão, Antônio Felipe

; Dracoulakis, Marianna Deway Andrade

; Hoff, Ana Oliveira

; Assunção, Bruna Morhy Borges Leal

; Novis, Yana

; Testa, Laura

; Alencar Filho, Aristóteles Comte de

; Cruz, Cecília Beatriz Bittencourt Viana

; Pereira, Juliana

; Garcia, Diego Ribeiro

; Nomura, Cesar Higa

; Rochitte, Carlos Eduardo

; Macedo, Ariane Vieira Scarlatelli

; Marcatti, Patricia Tavares Felipe

; Mathias Junior, Wilson

; Wiermann, Evanius Garcia

; Val, Renata do

; Freitas, Helano

; Coutinho, Anelisa

; Mathias, Clarissa Maria de Cerqueira

; Vieira, Fernando Meton de Alencar Camara

; Sasse, André Deeke

; Rocha, Vanderson

; Ramires, José Antônio Franchini

; Kalil Filho, Roberto

Arquivos Brasileiros de Cardiologia

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Nov 2020, Volume 115 Nº 5 Páginas 1006 - 1043

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20201006 4180 downloads

Assessment of resistance in common bean to Fusarium oxysporum f. sp. phaseoli using different inoculation and evaluation methods
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Paulino, Jean Fausto de Carvalho

; Almeida, Caléo Panhoca de

; Gonçalves, Gabriel de Moraes Cunha

; Bueno, César Júnior

; Carbonell, Sérgio Augusto Morais

; Chiorato, Alisson Fernando

; Bechimol-Reis, Luciana Lasry

Crop Breeding and Applied Biotechnology

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2020, Volume 20 Nº 3 elocation e337620311

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to evaluate the resistance of three common bean genotypes (BRS Estilo, A211 and Mortiño) to the Fusarium oxysporum f. sp. phaseoli using different inoculation methods such as the root-dip, the colonized toothpick, and the agar-based disk-diffusion. After 35 days of inoculation, the disease severity was assessed by vascular discoloration on the stem of the plants by two methods: using a scoring scale and measuring the length of the discoloration with the aid of a millimeter ruler (cm). Results showed that the root-dip was the most effective inoculation method. As for the method of assessing the disease severity, the scoring scale was the best one, in addition to being easier for evaluating large amounts of common bean lines.

https://doi.org/10.1590/1984-70332020v20n3n45 479 downloads

Addendum for the paper "Two new species of Emersonella (Hymenoptera: Eulophidae: Entedoninae) from the Brazilian Atlantic forest"
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Alvarenga, Thiago M.

; Costa, Valmir A.

; Hansson, Christer

; Carvalho, César F.

Zoologia (Curitiba)

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Dez 2015, Volume 32 Nº 6 Páginas 538 - 538

Resumo: En | Texto: En | PDF: En

ABSTRACT The purpose of this short communication is to provide information on the depository for the primary types of the new species described by Alvarenga et al. (2015) and thus validate the names suggested therein. The depository for the primary types of the new species Emersonella appendigaster Alvarenga, Costa & Hansson, 2015 and Emersonella frieirocostai Alvarenga, Costa & Hansson, 2015 is the Museu de Zoologia, Universidade de São Paulo, São Paulo, Brazil (MZSP).

https://doi.org/10.1590/s1984-46702015000600012 1015 downloads

Development and predatory capacity of Chrysoperla externa (Neuroptera: Chrysopidae) larvae at different temperatures
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FONSECA, ALYSSON R.

; CARVALHO, CÉSAR F.

; CRUZ, IVAN

; SOUZA, BRÍGIDA

; ECOLE, CARLOS C.

Revista Colombiana de Entomología

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Jun 2015, Volume 41 Nº 1 Páginas 4 - 11

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Chrysoperla externa (Neuroptera: Chrysopidae) es uno de los enemigos naturales del pulgón de la hoja del maíz Rhopalosiphum maidis (Hemiptera: Aphididae) y tiene potencial aplicación en el control biológico contra esta plaga. Fue investigado el efecto de la temperatura sobre el desarrollo y capacidad depredadora de C. externa alimentada con ninfas de R. maidis. Huevos frescos del crisópido fueron mantenidos a 15, 20, 25 ó 30 °C bajo una humedad relativa de 70% y 12 h fotofase. Fueron evaluadas la duración del período embrionario, la duración y viabilidad de la fase larvaria (primer, segundo y tercer estadio), fases de prepupa, pupa y adulta del depredador. La duración de todas las etapas del desarrollo se redujo al aumentar la temperatura, mientras que la viabilidad de todas las fases alcanzó el 100 % a los 20 y 25 °C. El umbral de temperatura y el valor de la constante térmica K obtenidos para el ciclo de vida completo (de huevo a adulto) fueron 10,7 ºC y 377,8 grados-día, respectivamente. Independiente de la temperatura, el consumo de ninfas de R. maidis por C. externa aumentó con el desarrollo de las larvas. A los 20 y 25 °C, el número promedio de pulgones consumidos durante toda la fase larvaria alcanzó aproximadamente 350 especímenes. Se concluye que el desarrollo de las formas inmaduras de C. externa alimentada con R. maidis, así como la viabilidad y su capacidad depredadora, se favorecen a temperaturas entre 20 y 25 °C.

Chrysoperla externa (Neuroptera: Chrysopidae) is one of the natural enemies of the corn leaf aphid Rhopalosiphum maidis (Hemiptera: Aphididae) and has potential application in the biological control of that pest. The effect of' temperature on the development and the predatory capacity of this chrysopid fed with R. maidis nymphs was investigated. Fresh eggs of C. externa were maintained at 15, 20, 25 or 30 °C under 70% relative humidity and 12 h photophase, and the duration of the embryonic stage, as well as the duration and viability of the larval (first, second and third instars), prepupal, pupal and adult forms of the predator were evaluated. The duration of each of these stages decreased with increasing temperature, whilst the viabilities of all forms attained 100% at 20 and 25 °C. The threshold temperature and the value of the thermal constant K obtained for the complete life cycle (egg to adult) were, respectively, 10.7 °C and 377.8 degree-days. Independent of temperature, the consumption of R. maidis nymphs by C. externa increased as the larvae reached maturity. At 20 and 25 °C the average number of aphids consumed during the complete larval stage was maximal at approximately 350 specimens. It is concluded, therefore, that the development of the immature forms of C. externa fed on R. maidis as well as its viability and predatory capacity, were favored at temperatures between 20 and 25 °C.

931 downloads

Two new species of Emersonella (Hymenoptera: Eulophidae: Entedoninae) from the Brazilian Atlantic Forest
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Alvarenga, Thiago M.

; Costa, Valmir A.

; Hansson, Christer

; Carvalho, César F.

Zoologia (Curitiba)

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Abr 2015, Volume 32 Nº 2 Páginas 145 - 150

Resumo: En | Texto: En | PDF: En

Emersonella Girault, 1916 is a wasp genus including species that parasitize Chrysomelidae (Coleoptera) eggs, mainly Cassidinae. It occurs only in the Americas, and is primarily distributed in the Neotropical Region. In this paper two new species of Emersonella from the Atlantic Forest of southeastern Brazil are described and compared to similar species. Emersonella appendigastersp. nov. is characterized by an elongate last gastral tergite in female, thoracic dorsum flat, femora and tibiae yellowish in female, malar sulcus absent, frontal suture slightly down-curved laterally, eyes with scattered hairs, frons and vertex smooth, and propodeum smooth with small anteromedian foveae. Emersonella frieirocostaisp. nov. is characterized by an elongate gaster in the female, at most 1.3 times as long as mesosoma and pointed at apex, propodeum with two large anterolateral foveae, pronotum hardly visible in dorsal view, with posterior part smooth, transverse pronotal carina present and malar sulcus absent. Both species are egg parasitoids of Metriona elatior (Klug, 1820) (Chrysomelidae) which feeds on Solanum viarum Dunal (Solanaceae).

https://doi.org/10.1590/S1984-46702015000200005 1304 downloads

Prevalence of cardiovascular risk factors, the association with socioeconomic variables in adolescents from low-income region
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Nascimento-Ferreira, Marcus Vinícius

; Moraes, Augusto Cesar F. de

; Carvalho, Heraclito B.

; Moreno, Luis A.

; Gomes Carneiro, André Luiz

; M. dos Reis, Victor Manuel

; Torres-Leal, Francisco Leonardo

Nutrición Hospitalaria

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Jan 2015, Volume 31 Nº 1 Páginas 217 - 224

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Objectives: To estimate the prevalence of obesity, overweight, abdominal obesity and high blood pressure in a sample of adolescents from a low-income city in Brazil and to estimate the relationship with the socioeconomic status of the family, the education level of the family provider and the type of school. Methods: This cross-sectional study randomly sampled 1,014 adolescents (54.8% girls), between 14-19 years of age, attending high school from Imperatriz (MA). The outcomes of this study were: obesity and overweight, abdominal obesity and high blood pressure (systolic and/or diastolic). The independent variables were: socioeconomic status (SES) of the family, education level of the family provider (ELFP) and type of school. The confounding variables were: gender, age and physical activity level. Prevalence was estimated, and the association between the endpoints and the independent variables was analyzed using a prevalence ratio (PR), with a 95% confidence interval, estimated by Poisson regression. Results: The overall prevalence of obesity was 3.8%, overweight, 13.1%, abdominal obesity, 22.7% and high blood pressure, 21.3%. The adjusted analysis indicated that girls with high SES showed an increased likelihood to be overweight (PR=1.71 [95% IC: 1.13-2.87]), while private school boys had an increased likelihood of obesity (PR=1.79 [95% CI: 1.04-3.08]) and abdominal obesity (PR =1.64 [95% CI: 1.06-2.54]). Conclusion: The prevalence of CVDR is high in adolescents from this low-income region. Boys from private schools are more likely to have obesity and abdominal obesity, and girls with high SES are more likely to be overweight.

Objetivos: Estimar la prevalencia de obesidad y sobrepeso, obesidad abdominal y hipertensión arterial en una muestra de adolescentes pertenecientes a una ciudad de baja renta en Brasil y su relación con el nivel socioeconómico, nivel educativo de lo responsable de la familia y tipo de escuela. Métodos: Estudio transversal con una muestra de 1014 adolescentes (54,8% chicas), con edades entre 14-19 años, estudiantes de las escuelas de la ciudad de Imperatriz (Brasil), seleccionadas por un muestreo aleatorio. Las variables dependientes evaluadas son: obesidad general y sobrepeso, obesidad abdominal, y tensión arterial alta (sistólica y/o diastólica). Las variables independientes son: nivel socioeconómico de la familia (NSO), el nivel de educación de lo responsable de la familia (NERF) y tipo de escuela. Las variables de confusión son: sexo, edad y nivel de actividad física. La prevalencia fue estimada, y la asociación entre las variables dependientes y las variables independientes se analizaron mediante razón de prevalencia (RP), con intervalo de confianza (IC) del 95%, estimado por la regresión de Poisson. Resultados: La prevalencia de la obesidad general fue de 3,8%, sobrepeso 13,1%, obesidad abdominal 22,7% y la tesión arterial alta 21,3%. Las análisis ajustadas indicaron que las chicas con NSO alto tienen mayor probabilidad de tener sobrepeso (RP=1,71 [IC95%: 1,13 a 2,87]), y chicos de las escuelas privadas tienen más probabilidad de tener obesidad (RP=1,79 [IC95%: 1.04- 3,08]) y obesidad abdominal (RP=1,64 [IC95%: 1,06 a 2,54]). Conclusión: La prevalencia de los FRC es alta en adolescentes de una región de baja renta. Los chicos de las escuelas privadas son más propensos a tener obesidad y obesidad abdominal, y las chicas con NSO alto son más propensas a tener sobrepeso.

https://doi.org/10.3305/nh.2015.31.1.7511 232 downloads

10.

Synthesis and in vitro evaluation of new benzenesulfonamides as antileishmanial agents
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Borges, Julio C.

; Carvalho, Adriana V.

; Bernardino, Alice M. R.

; Oliveira, César D.

; Pinheiro, Luiz C. S.

; Marra, Roberta K. F.

; Castro, Helena C.

; Wardell, Solange M. S. V.

; Wardell, James L.

; Amaral, Veronica F.

; Canto-Cavalheiro, Marilene M.

; Leon, Leonor L.

; Genestra, Marcelo

Journal of the Brazilian Chemical Society

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Mai 2014, Volume 25 Nº 5 Páginas 980 - 986

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Este artigo descreve a síntese e a atividade antileishmania de novos derivados pirazolil benzenossulfonamídicos. Estes foram elucidados por métodos espectrométricos. Alguns compostos mostraram uma atividade in vitro significativa contra Leishmania amazonensis, destacando-se o derivado 1e. Nenhum dos derivados pirazolil benzenossulfonamídicos mostraram qualquer toxicidade em macrófagos murinos.

This paper describes the synthesis and the antileishmanial activity of new pyrazolyl benzenesulfonamide derivatives. These were elucidated by spectrometric methods. Some compounds showed a significant in vitro activity against Leishmania amazonensis, highlighting the derivative 1e. These pyrazolyl benzenesulfonamide derivatives did not show any toxicity in murine macrophage.

https://doi.org/10.5935/0103-5053.20140062 1388 downloads

11.

Evaluation of antifungal activity of seaweed extracts
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Peres, Julio Cesar Fernandes

; Carvalho, Luciana Retz de

; Gonçalez, Edlayne

; Berian, Luis Otávio Saggion

; Felicio, Joana D´arc

Ciência e Agrotecnologia

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Jun 2012, Volume 36 Nº 3 Páginas 294 - 299

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

As macroalgas marinhas, por estarem sujeitas a numerosas interações biológicas e, por vezes, a condições abióticas extremas, desenvolveram, entre outros mecanismos de defesa, a capacidade de produzir substâncias biologicamente ativas. Assim, esses organismos produzem, principalmente, terpenos e fenóis. Entre outras, a atividade antifúngica, por sua importância na saúde humana e animal e na preservação de produtos agrícolas, tem sido objeto de diversos estudos. No presente trabalho, esta atividade foi investigada em dez extratos de macroalgas marinhas, por ensaios de bioautografia direta, frente ao fungo Colletotrichum lagenarium e por ensaio de difusão em disco, frente ao Aspergillus flavus. Os organismos estudados foram os seguintes: Stypopodium zonale, Laurencia dendroidea, Ascophyllum nodosum, Sargassum muticum, Pelvetia canaliculata, Fucus spiralis, Sargassum filipendula, Sargassum stenophyllum, Laminaria hyperborea e Gracilaria edulis. Os extratos de S. zonale, L. dendroidea, P. canaliculata, S. muticum, A. nodosum e F. spiralis inibiram significativamente o crescimento de C. lagenarium, porém, não significativamente, o do fungo A. flavus. A presença de terpenos em todos esses extratos foi confirmada por cromatografia em camada delgada e a de compostos fenólicos, apenas nos extratos de P. canaliculata, A. nodosum e S. muticum. No estudo químico por cromatografia em coluna, seguido por análise em cromatógrafo a gás/espectrômetro de massas, foram identificados, em L. dendroidea, os terpenos neofitadieno, cartilagineol, elatol e obtusol e o éster hexadecanoato de etila. Esse é o primeiro relato sobre a atividade de extratos de algas frente a C. lagenarium, fungo de importância agrícola.

Seaweeds are subject to numerous biological interactions and sometimes to extreme abiotic conditions, so they have developed among other defense mechanisms, the ability to produce biologically active substances. Thus, these organisms produce mainly terpenes and phenols. Among others, the antifungal activity, due to its importance in human and animal health and the production of agricultural products, has been the subject of several studies. In the present work, this activity was investigated in ten seaweeds extracts, by direct bioautography assays, compared to Colletotrichum lagenarium and disk diffusion assay, compared to Aspergillus flavus. The organisms studied were: Stypopodium zonale, Laurencia dendroidea, Ascophyllum nodosum, Sargassum muticum, Pelvetia canaliculata, Fucus spiralis, Sargassum filipendula, Sargassum stenophyllum, Laminaria hyperborea and Gracilaria edulis. S. zonale, L. dendroidea, P. canaliculata, S. muticum, A. nodosum and F. spiralis extracts significantly inhibited the C. lagenarium growth, but not inhibited significantly the A. flavus growth. The presence of terpenes in all of these extracts was confirmed by thin layer chromatography whereas the presence of phenolic compounds was confirmed only in extracts of P. canaliculata, A. nodosum and S. muticum. In chemical study by column chromatography, followed by gas chromatography/mass spectrometry analysis, the terpenes neophytadiene, cartilagineol, obtusol elatol; and the ester ethyl hexadecanoate were identified in the L. dendroidea extract. This is the first report on the activity of seaweed extracts against C. lagenarium, a fungus bearing agricultural importance.

9267 downloads Citado 1 vez em SciELO

12.

The brazilian version of the Quality of Life Assessment of Growth Hormone Deficiency in Adults (QoL-AGHDA): Four-stage translation and validation
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Ribeiro-Oliveira Jr, Antônio

; Mol, Suélem Simão

; Twiss, James

; Alencar, Guilherme Asmar

; Miranda, Paulo Augusto Carvalho

; Naves, Luciana A.

; Azevedo, Monalisa F.

; Basílio, Flávia M. A.

; Boguszewski, César Luiz

; Nogueira, Kátia C.

; Vilar, Lúcio

; Koltowska-Haggstrom, Maria

; McKenna, Steve P.

Arquivos Brasileiros de Endocrinologia & Metabologia

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Dez 2010, Volume 54 Nº 9 Páginas 833 - 841

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

OBJETIVO: Este estudo relata o processo de adaptação da versão brasileira do questionário QoL-AGHDA (Quality of Life - Assessment of Growth Hormone Deficiency in Adults) para pacientes com deficiência do hormônio de crescimento (DGH). MATERIAIS E MÉTODOS: A tradução adotou a metodologia de duplo painel. O questionário foi testado por intermédio de entrevistas direcionadas com 16 pacientes com DGH. No estágio final, dados de 120 pacientes com DGH (81 com teste/reteste) foram analisados para consistência interna, confiabilidade teste/reteste, validade convergente e validade entre grupos conhecidos. RESULTADOS: Os grupos de tradução foram bem-sucedidos e a versão final foi adaptada seguindo sugestões obtidas das entrevistas com os 16 pacientes. O coeficiente alfa de Cronbach foi 0,90, confiabilidade teste/reteste 0,88, escores QoL-AGHDA se correlacionaram com o NHP (p < 0,01) e também com a saúde geral relatada pelos pacientes (p < 0,01). CONCLUSÕES: A adaptação do QoL-AGHDA para a população brasileira foi bem-sucedida, e a nova versão demonstrou ser válida e confiável.

OBJECTIVE: This study reports on the Brazilian Portuguese adaptation of the QoL-AGHDA (Quality of Life Assessment of Growth Hormone Deficiency in Adults) for use in adult growth hormone deficient (GHD) patients. MATERIALS AND METHODS: The translation process adopted the dual panel methodology. The questionnaire was tested through field-test interviews (16 GHD patients). In the final stage, data from 120 GHD patients (81 included in a test-retest analysis) were analyzed for internal consistency, test-retest reliability, convergent validity and validity among known groups. RESULTS: The translation panels were successful and the draft version was amended to improve the wording as a result of the field-test interviews. Cronbach's alpha was 0.90 and test-retest reliability 0.88. QoL-AGHDA scores had the expected pattern of association with NHP scale scores and QoL-AGHDA was able to differentiate significantly between patients based on patient-reported general health (p < 0.01) and QoL (p < 0.01). CONCLUSIONS: The adaptation of the QoL-AGHDA for a Brazilian population was successful and the adapted questionnaire was shown to be reliable and valid.

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13.

Avaliação de modelos digitais de elevação para aplicação em um mapeamento digital de solos
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Chagas, César S.

; Fernandes Filho, Elpídio I.

; Rocha, Márcio F.

; Carvalho Júnior, Waldir de

; Souza Neto, Nestor C.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Fev 2010, Volume 14 Nº 2 Páginas 218 - 226

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

No Brasil, normalmente os modelos digitais de elevação (MDEs) são produzidos pelos próprios usuários e pouca atenção tem sido dada às suas limitações, como fonte de informação espacial. Este estudo propôs avaliar diferentes MDEs para subsidiar a escolha do modelo apropriado para derivar atributos topográficos utilizados em um mapeamento digital de solos, por redes neurais artificiais. A avaliação constou da determinação da raiz quadrada do erro médio quadrático da elevação (RMSE); análise das depressões espúrias; comparação entre drenagem mapeada e drenagem numérica, curvas de nível derivadas e curvas de nível originais, e análise das bacias de contribuição derivadas. Os resultados obtidos demonstraram que apenas o RMSE não foi suficiente para avaliar a qualidade desses modelos. O MDE, derivado de curvas de nível (CARTA, obtido com a utilização do módulo TOPOGRID) apresentou qualidade superior aos MDEs derivados de sensores remotos (ASTER e SRTM). A análise qualitativa também identificou que o MDE CARTA é superior aos demais, pois estes apresentaram grande quantidade de erros que podem comprometer o estabelecimento das relações entre atributos do terreno e as condições locais de solos.

In Brazil, the digital elevation models (DEMs) are usually produced by users themselves and little attention has been given to their limitations as source of spatial information. The objective of this study was to evaluate different DEMs to help in choosing an appropriate model to derive topographical attributes used in a digital soil mapping based on a neural networks approach. The evaluation consisted of the following analysis: determination of root mean square error (RMSE) of elevation; analysis of the spurious depressions; comparison between mapped drainage and numeric drainage and between derived contour lines and original contour lines; and analysis of the derived contribution basins. The results demonstrated that RMSE was not enough to evaluate the quality of these models. DEMs derived from contour lines (CARTA, obtained using the TOPOGRID module) presented better quality than those derived from remote sensors (ASTER and SRTM). These presented great amount of errors that can negatively affect the establishment of relationships between topographical attributes and local conditions of soils.

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14.

Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
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Poletto, Andréia B.

; Wasko, Adriane P.

; Oliveira, Claudio

; Azevedo, Alexandre

; Carvalho, Robson F.

; Silva, Maeli Dal Pai

; Foresti, Fausto

; Martins, Cesar

Genetics and Molecular Biology

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2008, Volume 31 Nº 1 Páginas 325 - 356

Resumo: En | Texto: En | PDF: En

Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The evolutionary relationships between the Nile tilapia actin genes and diverse other organisms is discussed.

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15.

Toxicidade de produtos fitossanitários utilizados na cultura do cafeeiro a larvas de Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) e efeitos sobre as fases subseqüentes do desenvolvimento do predador
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Silva, Rogério A.

; Carvalho, Geraldo A.

; Carvalho, César F.

; Reis, Paulo R.

; Pereira, Antônio M.A.R.

; Cosme, Luciano V.

Neotropical Entomology

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Dez 2005, Volume 34 Nº 6 Páginas 951 - 959

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Avaliou-se a seletividade fisiológica de alguns produtos fitossanitários utilizados em cafeeiro a larvas de Chrysoperla externa (Hagen) e seus reflexos nas fases subseqüentes do desenvolvimento do predador. Os tratamentos avaliados, em g i.a./L de água foram: 1- endosulfam (Thiodan 350 CE - 1,75), 2- clorpirifós (Lorsban 480 CE - 1,2), 3- betaciflutrina (Turbo 50 CE - 0,013), 4- enxofre (Kumulus 800 PM - 4,0), 5- azociclotina (Peropal 250 PM - 0,31), 6- oxicloreto de cobre (Cuprogarb 500 PM - 5,0) e 7- testemunha (água). As pulverizações foram realizadas em larvas de primeiro, segundo e terceiro ínstares de C. externa, por meio de torre de Potter. Em seguida, as larvas foram individualizadas em tubos de vidro e mantidas em câmara climatizada regulada a 25 ± 2ºC, UR de 70 ± 10% e fotofase de 12h. A toxicidade dos produtos foi calculada em função do seu efeito total (E) e categorizada conforme escala proposta pela IOBC. Clorpirifós e betaciflutrina foram nocivos a larvas de primeiro ínstar (E > 99%) e os demais foram seletivos. Clorpirifós foi também tóxico a larvas de segundo e terceiro ínstares, sendo os demais compostos inócuos ao predador (E < 30%). Nenhum dos produtos avaliados afetou a duração e sobrevivência de pupas, ou a razão sexual e fase adulta dos indivíduos provenientes de larvas tratadas. Endosulfam, enxofre, azociclotina e oxicloreto de cobre foram seletivos para larvas de primeiro, segundo e terceiro ínstares de C. externa e não afetaram as fases subseqüentes do desenvolvimento desse crisopídeo, podendo ser utilizados no manejo de pragas na cultura do cafeeiro.

The objective was to evaluate the selectivity of pesticides used in coffee crops to larvae of Chrysoperla externa (Hagen) and their effects on the subsequent developmental stages of the predator. The treatments in g a.i./L of water were: 1 - endosulfan (Thiodan 350 CE - 1.75), 2 - chlorpyrifos (Lorsban 480 CE - 1.2), 3 - betacyfluthrin (Turbo 50 CE - 0.013), 4 - sulphur (Kumulus 800 PM - 4.0), 5 - azocyclotin (Peropal 250 PM - 0.31), 6 - copper oxichloride (Cuprogarb 500 PM - 5.0) and 7 - control (water). The products were sprayed on first, second and third-instar larvae using a Potter's tower. The larvae were individualized in glass tubes and maintained at 25 ± 2ºC, RH of 70 ± 10% and 12h photophase. The toxicity of the pesticides was calculated based in their total effect (E) and classified according to recommendations of IOBC. Chlorpyrifos and betacyfluthrin were harmful to first-instar larvae (E > 99%). Endosulfan, sulphur, azocyclotin and copper oxichloride were harmless to first-instar larvae and the others were selective. Chlorpyrifos was also toxic to second and third-instar larvae, and the other compounds were selective (E < 30%). None of the pesticides affected the duration and survival rate of pupae or the sex ratio of the adults originated from treated larvae. Endosulfan, sulphur, azocyclotin and copper oxichloride were harmless to the larval stage of C. externa and did not affect the subsequent stages, so that they can be recommended in IPM programs for the coffee crop.

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