Pesquisa | SciELO

Abstract Chelates are nutrient-rich compounds that enhance the condition of plant tissues as micronutrients. Micronutrient deficiencies particularly iron (Fe) and zinc (Zn) leads to various problems for plant including chlorosis and necrosis etc. An adequate intake of Fe and Zn etc. is required by the human body. Biofortification of cereals with Fe and Zn is seen as a cost-effective solution to the problem of Fe and Zn deficiencies as well. In recent decades, many chelating compounds have been established and incorporated into agricultural systems. The most recent formulation involves the use of amino acids synthesized with one or more nutrient ions to improve fertilizer efficiency and better respond to environmental conservation. In addition to its primary function as a source of micronutrients, aminochelled are an active nitrogen (N) stimulant in plant nutrition, preventing the negative effects of basic N fertilizers like urea. The use of amino chelates, rather than just chemical fertilizers, has been shown to provide better production and quality as well as higher nutritional concentrations in several experiments. Furthermore, this review sheds light on various aspects of amino chelates fertilizers including types, history, and their effects on agricultural crops. In spite of amino chelates fast dominance in many countries’ fertilizer countries, there is not enough scientific data and knowledge on the specific reactions of plants to biotic and abiotic stresses from amino fertilizers. nutrientrich rich micronutrients (Fe (Zn etc body costeffective cost effective decades systems conservation (N nutrition urea experiments Furthermore types history crops countries

Resumo Os quelatos são compostos ricos em nutrientes que melhoram a condição dos tecidos vegetais como micronutrientes. Deficiências de micronutrientes, particularmente ferro (Fe) e zinco (Zn), levam a vários problemas para as plantas, incluindo clorose e necrose, etc. A ingestão de uma quantidade adequada de Fe e Zn, etc., é exigida pelo corpo humano. A biofortificação de cereais com Fe e Zn também é vista como uma solução econômica para o problema das deficiências de Fe e Zn. Nas últimas décadas, muitos compostos quelantes foram estabelecidos e incorporados em sistemas agrícolas. A formulação mais recente envolve o uso de aminoácidos sintetizados com um ou mais íons nutrientes para melhorar a eficiência do fertilizante e responder melhor à conservação ambiental. Além de sua função primária como fonte de micronutrientes, os aminoquelados são um estimulante de nitrogênio (N) ativo na nutrição das plantas, evitando os efeitos negativos de fertilizantes nitrogenados básicos como a ureia. O uso de aminoquelatos, ao invés de apenas fertilizantes químicos, tem mostrado proporcionar melhor produção e qualidade, bem como maiores concentrações nutricionais em vários experimentos. Além disso, a presente revisão lança luz sobre vários aspectos dos fertilizantes aminoquelatos, incluindo tipos, história e seus efeitos nas culturas agrícolas. Apesar do domínio rápido dos aminoquelatos em muitos países de fertilizantes, não há dados científicos suficientes e conhecimento sobre as reações específicas das plantas aos estresses bióticos e abióticos dos fertilizantes amino. micronutrientes (Fe , (Zn) necrose etc humano décadas agrícolas ambiental N (N ureia químicos qualidade experimentos disso tipos amino (Zn

https://doi.org/10.1590/1519-6984.271055

Diversidad genética del Complejo de Mycobacterium tuberculosis: implicaciones clínicas y epidemiológicas
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Gómez-Velasco, Anaximandro

; Molina-Torres, Carmen Amelia

; Vera-Cabrera, Lucio

; Bencomo-Alerm, Alied

; Muñoz-Jiménez, Sergio G.

; Sánchez-Pérez, Héctor Javier

; Gordillo-Marroquín, Cristina

; Córdova-Solis, Christian D.

; Enríquez-Ríos, Natán

; Nuñez Ovilla, Dorian Buridán

; Sánchez-Oropeza, Carlos

; Nájera Ortíz, Juan Carlos

; Rodríguez Suárez, René Armando

TIP. Revista especializada en ciencias químico-biológicas

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2023, Volume 26 elocation e563

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract The Mycobacterium tuberculosis complex (MTBC) is a successful human pathogen. In 2022, approximately 10.6 million people have developed the active phase of the disease, and around 1.6 million people died due to tuberculosis (TB). The deciphering of the genome of M. tb has helped us to understand its evolutionary history and the causes underlying its worldwide spread. Using various genotyping methods and whole-genome sequencing techniques indicate that the M. tb complex is grouped in nine lineages, some are specifically adapted to humans, while others are animal-adapted lineages. The presence of most of the lineages in Africa makes this continent the most likely place of MTBC origin. Studies of the genome of M. tb have also assisted in the identification of genes involved in its virulence, pathogenicity, and the long co-evolutionary process with its main host, the human being. In this review of selected literature, we describe the genotypic and phenotypic diversity of the MTBC and its clinical and epidemiological implications.

Resumen El complejo de Mycobacterium tuberculosis (CMTB) es uno de los patógenos con mayor éxito evolutivo por su alta transmisión en las poblaciones humanas. En el 2022, la OMS estimó que aproximadamente 10.6 millones de personas desarrollaron la fase activa de la enfermedad, principalmente la forma pulmonar (tuberculosis pulmonar, TBP), y alrededor de 1.6 millones, más, fallecieron debido a la tuberculosis (TB). Diversas técnicas con base en el genotipo y la secuencia del genoma de Mycobacterium tuberculosis (M. tb) han permitido entender su historia evolutiva y comprender las causas de su propagación mundial. El análisis y las comparaciones de la secuencia de los genomas completos indican que el CMTB se compone de nueve linajes, algunos están específicamente adaptados a los humanos y otros a los animales. La presencia de la mayoría de los linajes en África, hace de este continente el origen del CMTB. Los estudios del genoma del CMTB también han hecho posible la identificación de los genes implicados en su virulencia y patogenicidad, así como en el largo proceso co-evolutivo con su principal hospedero, el ser humano. En esta revisión se describe la diversidad genotípica y fenotípica del CMTB y sus implicaciones clínicas y epidemiológicas.

https://doi.org/10.22201/fesz.23958723e.2023.563

Diagnóstico y tratamiento de tromboembolia pulmonar. Revisión bibliográfica
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Arias-Rodríguez, Fabián D.

; Armijos-Quintero, Daniel A.

; Beltrán-Vinueza, Pamela A.

; Córdova-Macías, Daniela V.

; Guadamud-Loor, Jorge X.

; Osejos-Moreira, Walter D.

; Rojas-Cadena, Marlon G.

; Vega-Bustamante, Melany K.

Revista mexicana de angiología

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Set 2022, Volume 50 Nº 3 Páginas 96 - 109

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen La tromboembolia pulmonar (TEP) generalmente es debida a una trombosis venosa profunda, estas en conjunto representan las dos condiciones conocidas como tromboembolia venosa (TEV), que ocurre debido a elementos predisponentes que incluyen daño endotelial, estasis venosa y estados de hipercoagulabilidad, conocidos como la tríada de Virchow. Es más común en hombres y la incidencia aumenta con la edad. La TEV es una condición potencialmente mortal si no se trata de manera oportuna debido a que tiene una tendencia natural hacia la recurrencia temprana de la embolia pulmonar que puede conducir al desarrollo de una insuficiencia ventricular derecha severa. Ante una sospecha de TEP se sugiere un enfoque que combina la evaluación de probabilidad clínica pretest acompañada de la prueba del dímero D y por imagen mediante la angiotomografía pulmonar computarizada. El manejo se centra en la estabilización del paciente y anticoagulación, según el riesgo de hemorragia.

Abstract Pulmonary thromboembolism (PE) is usually due to deep vein thrombosis (DVT), these together represent the two conditions known as Venous Thromboembolism (VTE), it occurs due to predisposing elements that include endothelial damage, venous stasis and hypercoagulable states, collectively known as Virchow’s Triad. It is more common in men and the incidence increases with age. PE is a life-threatening condition if not treated promptly because it has a natural tendency toward early recurrence of pulmonary embolism that can lead to the development of severe right ventricular failure. When PE is suspected, an approach is suggested that combines the evaluation of pre-test clinical probability accompanied by the D-Dimer test and imaging by computed tomography pulmonary angiography. Management focuses on patient stabilization and anticoagulation, depending on the risk of bleeding.

https://doi.org/10.24875/rma.22000018 121 downloads

CONDICIONES PARA LA TRANSESTERIFICACIÓN ENZIMÁTICA DE ACEITE DE JATROPHA CURCAS CON UN SÓLIDO FERMENTADO
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Alcala-Galiano Morell, Diana D.

; Córdova López, Jesús A.

; Romero Borbón, Evelyn

; Rodríguez González, Jorge Alberto

; Cosío Cuadros, Ricardo

; Ramos-Sánchez, Luis B.

Centro Azúcar

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Jun 2021, Volume 48 Nº 2 Páginas 56 - 67

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Introduction: The main drawback of enzymatic biodiesel production is biocatalyst high prices. Fermented solids with lipase activity use mitigates costs, but being a set of lipase enzymes, the best reaction conditions are unknown. Objective: To determine the best conditions for particle size, stirring speed and ethanol / oil molar ratio, for the reaction to produce ethyl esters, using Jatropha curcas oil and ethanol as substrates and, as a biocatalyst, a fermented solid with activity lipase. Materials and Methods: Two biocatalysts were initially contrasted. The stirring speed, the particle size and the alcohol / oil molar ratio were studied in the transesterification reaction. The reaction products were measured by high performance thin layer chromatography. Results and Discussion: Rhizopus homothallicus fermented solid was selected as biocatalyst, with an activity of 75.39 IU gMS -1 and a high productivity of 4.19 IU gMS -1 h-1. It was established that the diffusional mass transfer resistances were minimized, using 200 rpm and a particle sizes range of 0.149 to 0.297 mm. It was found that for 3:1 and 4:1 molar ratio, there are no significant differences in conversion degree, reaching 77.69 % of conversion. However, for 6:1 ratio, the conversion was affected, due to enzyme inhibition processes. Conclusions: It was considered that used biocatalyzer under the established reaction conditions has a good potential to be used in biodiesel synthesis.

Resumen Introducción: La producción de biodiesel enzimático, tiene como principal inconveniente, altos precios del biocatalizador. La utilización de sólidos fermentados con actividad lipasa, mitigan los costos, pero al ser un conjunto de enzimas lipasas se desconoce las mejores condiciones de reacción. Objetivo: Determinar las mejores condiciones de tamaño de partícula, velocidad de agitación y relación molar etanol/aceite, para la reacción de producción de etil-ésteres, utilizando como sustratos: aceite de Jatropha curcas y etanol y, como biocatalizador, un sólido fermentado con actividad lipasa. Materiales y Métodos: Se contrastaron inicialmente dos biocatalizadores. Se estudiaron la velocidad de agitación, el tamaño de partícula y la relación molar alcohol/aceite, en la reacción de transesterificación. Los productos de la reacción se midieron, mediante cromatografía de capa fina de alta resolución. Resultados y Discusión: Se seleccionó como biocatalizador el sólido de Rhizopus homothallicus, con una actividad de 75,39 UI gMS -1 y una alta productividad de 4,19 UI gMS -1 h-1. Se estableció que las resistencias a la transferencia de masa difusional se minimizan, al utilizar 200 rpm y un intervalo de tamaños de partícula de 0,149 a 0,297 mm. Se comprobó que para la relación molar 3:1 y 4:1, no hay diferencias significativas en el porciento de conversión, obteniéndose valores de 77,69 %. Sin embargo, para la relación 6:1, se afectó la conversión, debido a los procesos de inhibición de la enzima. Conclusiones: Se estima que el biocatalizador utilizado en las condiciones de reacción establecidas tiene un buen potencial para ser usado en la síntesis de biodiesel.

Mantle cell lymphoma may have a different clinical course in Mexican Mestizos: Real-world data from a single center
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Córdova-Ramírez, Alejandra C.

; Sánchez-Valledor, Luisa F.

; Colótero, Gerardo

; Rivera-Alvarez, Montserrat

; Elías-de-la-Cruz, Gilberto D.

; Murrieta-Alvarez, Montserrat

; Cantero-Fortiz, Yahveth

; León-Peña, Andrés

; Olivares-Gazca, Juan C.

; Ruiz-Delgado, Guillermo J.

; Ruiz-Argües, Guillermo J.

Revista de investigación clínica

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Abr 2021, Volume 73 Nº 2 Páginas 94 - 99

Resumo: En | Texto: En | PDF: En

ABSTRACT Background: The biology of some hematological diseases varies among different populations. No previous studies have evaluated the clinical behavior of mantle cell lymphoma (MCL) in México. Objective and Methods: This is a retrospective review of MCL cases seen in Mexico from January 2003 to June 2020. A total of 12 cases were identified. Results: There were nine males and three females; median age was 56 years. Eight patients had a high MCL international prognostic index score, one was intermediate, and three were low. Five patients had circulating malignant monoclonal cells. Initial treatment included rituximab, cyclophosphamide, daunorubicin, vincristine, and prednisone (R-CHOP) and CHOP. Subsequent treatment included hematopoietic stem cell transplantation in five patients; two were given maintenance therapy. Splenectomy was done in four patients. Median overall survival (OS) for all the patients has not been reached and exceeds 162 mos: OS at 162 mos was 56%. Achieving a complete remission (CR) after the first treatment was a significant prognostic factor, with a median OS exceeding 141 mos in patients achieving CR, and 16 mos among those not achieving CR (p = 0.0006). Conclusion: Some of MCL patients in Mexico have an indolent clinical course, particularly patients who achieve a CR to initial treatment and who undergo splenectomy.

https://doi.org/10.24875/ric.20000381 152 downloads

Características clínicas y resultado del tratamiento de niños con leucemia linfoblástica aguda e hiperleucocitosis
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Rodríguez-Aguirre, Manuel I.

; Hernández-Cuéllar, Esperanza

; Espinoza-Román, Víctor H.

; Acevedo-Segura, Virna G.

; Lagos-Córdova, Elda Y.

; Vera-Morales, Carlos D.

; Vázquez-García, Rubén E.

; Palomo-Collí, Miguel Á.

Gaceta mexicana de oncología

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2021, Volume 20 Supl. 1 Páginas 23 - 27

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Introduction: Hyperleukocytosis in children with acute lymphoblastic leukemia (ALL) has been associated with early morbidity and mortality. Clinical Case: We analyzed clinical data from 6 patients with ALL. We documented adverse events within the first 28 days in patients with a white blood cell (WBC) count ≥100 × 10(9)/l and reviewed their management. The most frequent biochemical alterations were hyperuricemia and hyperglycemia There were no severe complications or deaths. Conclusions: With improved management, including supportive care, early mortality in patients with ALL and hyperleukocytosis could be decreased remarkably.

Resumen Introducción: La hiperleucocitosis en niños con leucemia linfoblástica aguda (LLA) se ha asociado con morbimortalidad temprana. Constituye una urgencia oncológica. Caso clínico: Revisamos datos de seis pacientes con LLA. Documentamos las complicaciones relacionadas con el tratamiento dentro de los primeros 28 días en pacientes con un recuento de glóbulos blancos ≥ 100 × 109/l. Las alteraciones bioquímicas más frecuentes fueron hiperuricemia e hiperglucemia. No hubo complicaciones graves ni muertes. Conclusiones: El tratamiento preventivo, la terapia de apoyo y el diagnóstico temprano pueden reducir la mortalidad temprana en pacientes con LLA e hiperleucocitosis.

https://doi.org/10.24875/j.gamo.21000031 74 downloads

COVID-19 with and without pneumonia: clinical outcomes in the internal medicine ward
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Melendi, Santiago E.

; Pérez, María M.

; Salas, Cintia E.

; Haedo, Mariana F.

; Xavier, Franco B.

; Navarrete, Jandry D. Saltos

; Aguirre, Camila

; Baleta, María L.

; Balsano, Facundo J.

; Caldano, Mariano G.

; Colignon, María G.

; De Oliveira Brasil, Thayana

; De Wolodimeroff, Nicolás

; Déramo Aquino, Andrea I.

; Fernández De Córdova, Ana G.

; Fontan, María B.

; Galvagno, Florencia I.

; Iturrieta Araya, Noelia S.

; Mollinedo Cruz, Volga S.

; Olivero, Agustín

; Pestalardo, Ignacio

; Ricciardi, María

; Vera Rueda, María L.

; Villaverde, María C.

; Mauri, Marcela Lauko

; Ujeda, Carlos

; Leis, Rocío

Medicina (Buenos Aires)

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Dez 2020, Volume 80 Supl. 6 Páginas 56 - 64

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract The clinical features of COVID-19 differ substantially upon the presence (or absence) of viral pneumonia. The aim of this article was to describe the clinical characteristics of COVID-19 patients admitted to the Internal Medicine ward, as divided into those with and without pneumonia. This single-center prospective cohort study was conducted in a tertiary teaching public hospital in Buenos Aires City named Hospital General de Agudos Carlos G. Durand. Baseline data collection was performed within 48 hours of admission and patients were followed until discharge or in-hospital death. Epidemiological, clinical, laboratory, and radiological characteristics together with treatment data were obtained from the medical records. Of the 417 included, 243 (58.3%) had pneumonia. Median age was 43 years (IQR:32-57) and 222 (53.2%) were female. The overall crude case-fatality rate was 3.8%. None of the COVID-19 patients without pneumonia developed critical disease, required invasive mechanical ventilation nor died during hospitalization. However, 7 (4%) developed severe disease during follow-up. Among patients with COVID-19 pneumonia, in-hospital mortality rate was 6.6%, severe disease developed in 81 (33.3%), critical disease in 23 (9.5%), and 22 (9.1%) were admitted to the intensive care unit. A largely good prognosis was observed among COVID-19 patients without pneumonia, still, even among this group, unfavorable clinical progression can develop and should be properly monitored. Critical illness among patients with COVID-19 pneumonia was frequent and observed rates from this cohort provide a sound characterization of COVID-19 clinical features in a major city from South America.

Resumen Las características clínicas del COVID-19 difieren sustancialmente según la presencia (o ausencia) de neumonía viral. El objetivo de este artículo fue describir las características clínicas de los pacientes con COVID-19 internados en el servicio de Clínica Médica, divididos en pacientes con y sin neumonía. Fue un estudio de cohorte prospectivo, con base en un único centro, realizado en un hospital público de la ciudad de Buenos Aires: Hospital General de Agudos Carlos G. Durand. La recolección basal de datos se realizó dentro de las 48 horas del ingreso y los pacientes fueron seguidos hasta el alta o la muerte hospitalaria. Las características epidemiológicas, clínicas, de laboratorio y radiológicas junto con los datos del tratamiento se obtuvieron de la historia clínica. De los 417 incluidos, 243 (58.3%) tenían neumonía. La mediana de edad fue de 43 años (RIC: 32-57) y 222 (53.2%) eran mujeres. La tasa global de letalidad fue del 3.8%. Ninguno de los pacientes con COVID-19 sin neumonía desarrolló enfermedad crítica, requirió ventilación mecánica invasiva ni falleció durante la hospitalización. Sin embargo, 7 (4%) desarrollaron enfermedad grave durante el seguimiento. Entre aquellos con neumonía COVID-19, la tasa de mortalidad hospitalaria fue del 6.6%, se desarrolló enfermedad grave en 81 (33.3%), enfermedad crítica en 23 (9.5%) y 22 (9.1%) fueron trasladados a la unidad de cuidados intensivos. Los pacientes con COVID-19 sin neumonía presentaron buen pronóstico; sin embargo, incluso en este grupo, se observaron algunos con progresión clínica desfavorable, por lo que se requirió seguimiento adecuado. En los pacientes con neumonía por COVID-19, el desarrollo de enfermedad crítica fue frecuente y las tasas observadas en esta cohorte proporcionan una caracterización sólida de las características clínicas de los pacientes con COVID-19 en una importante ciudad de América del Sur.

174 downloads

Evaluación de la respuesta productiva e inmune en juveniles de camarón Litopenaeus vannamei alimentado con mezclas probióticas
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Campa-Córdova, Angel I.

; Yenni-Morales, Cristóbal

; Guzmán-Murillo, María A.

; Aguirre-Guzman, Gabriel

Revista de biología marina y oceanografía

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Ago 2020, Volume 55 Nº 1 Páginas 73 - 78

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract: Shrimp diets with mixtures of probiotics (bacilli or yeasts) were evaluated on the production response and immunological effect on juvenile shrimp (Litopenaeus vannamei). The results showed a significant effect in growth, food consumption and feed conversion rate when probiotic diet was used. In addition, experimental diets with yeast mixture [C. insectorum (DH5), D. hansenii (DH6, and L1)] had a significantly higher amount of circulating haemocytes than those with bacilli [B. tequilensis (YC5-2), B. endophyticus (YC3-b) and B. endophyticus (C2-2)]. Shrimp fed both experimental diets (3x107 CFU mL-1) had a significantly higher growth and immune parameters when compared with the commercial diet. Both elements can contribute to the sustainable development and health of shrimp farming.

https://doi.org/10.22370/rbmo.2020.55.1.2394 303 downloads

Drift Distance in Aircraft Glyphosate Application Using Rice Plants as Indicators
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CÓRDOVA, R.A.

; TOMAZETTI, M.

; REFATTI, J.P.

; AGOSTINETTO, D.

; AVILA, L.A.

; CAMARGO, E.R.

Planta Daninha

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2020, Volume 38 elocation e020223422

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O presente estudo teve como objetivo avaliar o potencial de deriva de glifosato durante aplicação aérea mediante o uso de plantas de arroz como sentinelas, a fim de determinar o efeito da deriva na cultura do arroz irrigado. Para isso, foi realizado um experimento em campo utilizando delineamento inteiramente casualizado com quatro repetições, avaliando diferentes distâncias [testemunha (sem aplicação), 0; 12,5; 25; 50; 75; 100; 150; 200; 300; e 400 m] a partir do local de aplicação. O experimento foi realizado nas Granjas 4 Irmãos, localizada no município de Rio Grande, RS. A dose de aplicação de glifosato foi de 1.920 g e.a. ha-1 (Roundup Transorb®, sal de isopropilamina de glifosato 480 g e.a. L-1). Para estimação da taxa de deriva nas plantas sentinelas, foi elaborada uma curva de dose-resposta, aplicando doses crescentes de glifosato em plantas de arroz e avaliando o nível de injúria. As taxas de deriva estimadas mediante o nível de injúria em plantas sentinelas foram de 14% (150 m), 13% (200 m) e 5% (400 m). Foi observado morte de plantas nas distâncias entre 0 a 50 m, enquanto em distâncias entre 75 e 150 m a taxa de sobrevivëncia foi de 25 a 50%, causando redução na produtividade. Já nas distâncias entre 200 e 400 m não houve redução na produtividade quando comparada com a testemunha, inclusive quando os níveis de injúria atingiram de 52 a 82%. Dessa forma, conclui-se que a deriva de glifosato de 5% atingiu até 400 m da faixa de aplicação. Considerando a recomendação de deriva zero, distâncias maiores que 400 m deverão ser adotadas para evitar sintomas em plantas de arroz. Sugere-se a utilização de distâncias maiores que 400 m em estudos futuros.

ABSTRACT: This study aimed to evaluate the potential for glyphosate drift during aerial application using rice plants as sentinels, aiming to determine the effect of drift on irrigated rice crops. For this purpose, a field experiment was performed using an entirely randomized design with four replicates, evaluating different distances from the site of application [control (no application), 0, 12.5, 25, 50, 75, 100, 150, 200, 300, and 400 m]. The experiment was carried out at the Granjas 4 Irmãos farm, located in the Rio Grande city, Rio Grande do Sul State, Brazil. The glyphosate dose application was 1,920 g e.a. ha-1 (Roundup Transorb®, 480 g e.a. L-1 glyphosate isopropylamine salt). A dose-response curve was developed to estimate the drift rate in sentinel plants, by applying increasing glyphosate doses in rice plants and assessing the injury level. The drift rates estimated by the injury level in sentinel plants were 14% (150 m), 13% (200 m), and 5% (400 m). Death of the experimental units was observed for distances between 0 and 50 m, while in distances between 75 and 150 m, 25 to 50% of the plants survived, reducing productivity. In the distances between 200 and 400 m, there was no reduction in productivity when compared to the control, even when the injury levels reached 52 to 82%. Thus, we concluded that a 5% glyphosate drift reached up to 400 m from the application range. Considering the recommendation of zero drift, distances greater than 400 m should be adopted to avoid symptoms in rice plants. We suggest using distances of more than 400 m in future studies.

https://doi.org/10.1590/s0100-83582020380100048 1142 downloads

10.

MEJORA EN EL NIVEL DE CONOCIMIENTO, DESPUÉS DE UNA INTERVENCIÓN EDUCATIVA, EN RESUCITACIÓN CARDIOPULMONAR BÁSICA EN ESTUDIANTES DE MEDICINA EN PIURA
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Mejia, Christian R.

; García-Saavedra, Mario B.

; Benites-Flores, Irwing R.

; Ordinola-Calle, Deysi D.

; Jiménez-Núñez, Dalia

; Alvarado-Córdova, Roberto

; Valladares-Garrido, Mario J.

; Valladares-Garrido, Danai

; Talledo-Ulfe, Lincolth

Revista Científica Ciencia Médica

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2020, Volume 23 Nº 1 Páginas 8 - 14

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Introduction: Cardiopulmonary resuscitation is a set of maneuvers that must be performed systematically and simultaneously, to save lives. The person who performs them must have a solid theoretical and practical knowledge of the technique to be used. Objective: To determine the difference in theoretical-practical knowledge of Cardiopulmonary Resuscitation according to the type of educational intervention in medical students in Peru. Methods: Study of educational intervention, conducted in students from two Peruvian universities. The main variable was to measure the change in the level of theoretical-practical knowledge according to the type of instruction (theoretical, theoretical-practical or practical), measured with a previously validated questionnaire 1. We used the statistical chi-square test for categorical variables and ANOVA for the analysis of numerical variables versus categorical variables, adjusted by the Bartlett test. Results: According to the type of intervention received, the difference between theoretical final averages was not significant (p>0.05), but practical final averages did show differences between groups (p<0.001). In the multivariate analysis, differences were found in the practical knowledge of the practical instruction group (p=0.020) and theoretical-practical knowledge (p=0.001) with respect to the exclusive theoretical instruction group. Conclusion: Practical and theoretical-practical instruction generates a significant change in the level of knowledge. This must be taken into account when generating instruction programs, since many times only theoretical knowledge is insufficient for this type of learning.

535 downloads

11.

Peyronie's disease: Review of the pathology and current events in treatment
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Carrillo-Córdova, Luis D.

; Rodríguez-Valle, Edson D.

; Rodriguez-Robles, Jonathan A.

; Vitar-Sandoval, Jhonatan

; Calvo-Mena, Daniel

; Garduño-Segovia, Noe

; Aguilar-Aizcorbe, Samuel

; Reyes-Sánchez, Adair

; Acevedo-Garcia, Christian

; Jaspersen-Gastelum, Jorge

; Almanza-Gonzalez, Mario S.

Revista médica del Hospital General de México

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Dez 2019, Volume 82 Nº 4 Páginas 208 - 214

Resumo: En | Texto: En | PDF: En

Peyronie's disease is a medical condition characterized by an abnormal curvature of the penis, which presents a poorly understood pathophysiology, featuring abnormal fibroblasts of the tunica albuginea secondary to trauma or stemming from congenital causes. Currently, there are multiple therapeutic options, such as oral pharmacological treatments that either report poor results or partial improvements, as well as intralesional therapies viewed as controversial and surgery as a therapeutic choice. However, this can be implemented once the disease is in its stable phase. A number of alternatives can be elected regarding the needs and symptoms of patients.

https://doi.org/10.24875/hgmx.m19000017 144 downloads

12.

Epidemiology of tumors in dogs in the capital of the state of Mexico from 2002-2016
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García, E.

; Alpízar, A.

; Fajardo, R.

; Córdova, D.

; Pérez, L.

; Martínez, S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Ago 2019, Volume 71 Nº 4 Páginas 1085 - 1092

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Um estudo retrospectivo e transversal dos tumores caninos foi realizado na capital do Estado do México durante o período de 2002-2016. Desde esse período, 393 tumores foram remetidos. Epidemiologia descritiva foi feita com base em todos os dados: raça, idade, sexo e características do tumor. Em seguida, a associação de risco entre tumores cutâneos e não cutâneos com variáveis como sexo, raça e idade foi analisada por meio do teste exato de Fisher e odds ratio. Além disso, algumas características da população canina foram estudadas em uma pesquisa. De todos os tumores, os de pele foram os mais frequentes (59,0%). O grupo de tumores relacionados à radiação ultravioleta foi o mais comum (15,1%). A frequência de tumores no sexo feminino foi de 53%, no entanto os machos apresentaram maior risco de desenvolver tumores cutâneos (OR = 1,88; 1,24-2,84) (0,003). As raças puras foram as mais comuns (82,5%), e a faixa etária mais frequente foi >7 anos (54,7%). A pesquisa mostrou que os machos (53%) e a faixa etária de um a sete anos (62,1%) foram os mais frequentes. Conclusões: idade, raça e sexo foram identificados como os principais fatores de risco para a tumorigênese. Da mesma forma, os tumores cutâneos foram associados à exposição à radiação ultravioleta, provavelmente até a altura da localidade (2667m ANM).

ABSTRACT A retrospective and cross-sectional study of canine tumors was performed in the capital of State of Mexico from 2002-2016. Since then, 393 tumors were remitted. Descriptive epidemiology was made from all data: breed, age, sex, and tumor features. Then the risk association between cutaneous and non-cutaneous tumors with variables such as sex, breed, and age were analyzed through an exact Fisher test and Odds ratio. In addition, some characteristics of the canine population were studied under a survey. Of all tumors, skin tumors were the most frequent (59.0%). The group of tumors related to ultraviolet radiation was the most common (15.1%). The frequency of tumors in females was 53%, nevertheless, males had more risk to develop cutaneous tumors (OR=1.88, 1.24-2.84) (0.003). The Pure breeds were the most common (82.5%) and the most frequent age range was > 7 years (54.7%). The survey showed that males (53%) and the age range 1-7 years (62.1%) were the most frequent. Conclusions, age, breed, and sex were identified as the major risk factors for tumorigenesis. Likewise, skin tumors were associated with exposure to ultraviolet radiation, probably to the height of the locality (2667mamls).

https://doi.org/10.1590/1678-4162-10534 1255 downloads

13.

Changes of digestive enzymes in totoaba (Totoaba macdonaldi Gilbert, 1890) during early ontogeny
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Córdova-Montejo, Mabelyn

; Álvarez-González, Carlos A.

; López, Lus M.

; True, Conal D.

; Frías-Quintana, Carlos A.

; Galaviz, Mario A.

Latin american journal of aquatic research

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Mar 2019, Volume 47 Nº 1 Páginas 102 - 113

Resumo: En | Texto: En | PDF: En

ABSTRACT Totoaba macdonaldi is an endemic species which has been overexploited causing its population to decrease and to be cataloged as endangered. Understanding the larval period is the most critical stage in the development of marine fish farming. Our study aims to determine the changes in digestive enzyme activities during the early ontogeny of totoaba, using biochemical and electrophoretic techniques as SDS-PAGE. The results show that the acid protease had maximum activity between days 28 and 32 days post-hatching (DPH) when the Artemia was replaced by formulated food. Alkaline protease activity was detected at 2 DPH with maximal activity between 20 and 32 DPH; trypsin activity became active from 2 DPH; chymotrypsin activity increased at 6 DPH; leucine aminopeptidase activity was detected at 3 DPH, showing its maximal level at 22 DPH; carboxypeptidase A activity increased at 3 DPH; and lipase and amylase activities were detected at 2 DPH. Acid zymogram showed only one isoform (0.72 rf) from 2 DPH and increased in intensity from 8 DPH. For alkaline proteases, four isoforms were detected from yolk absorption (1 DPH), increasing to six isoforms (77.8, 47.3, 43.5, 21.0, 19.2, and 17.5 kDa) from 8 DPH onwards. Our results demonstrate that larvae of T. macdonaldi present characteristics typical of carnivore marine fish, showing the presence of alkaline digestive enzymes prematurely and the presence of offset pepsin. According to the results obtained in the present study, T. macdonaldi can be a juvenile beginning between 24 and 28 DPH, being the most appropriate day to perform replacement by artificial food.

https://doi.org/10.3856/vol47-issue1-fulltext-11 593 downloads

14.

Eficiencia de lectinas como inmunoindicadores en juveniles de camarón blanco, Litopenaeus vannamei (Malacostraca: Penaeidae)
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Martín-Manzo, Miriam Victoria

; Angulo, Carlos

; Zenteno-Galindo, Edgar

; Sierra-Beltrán, Arturo Pedro

; Agundis-Mata, Concepción

; Ascencio, Felipe

; Guzmán-Murillo, María Antonia

; Luis-Villaseñor, Irasema Elizabeth

; Pereyra, Alí

; Campa-Córdova, Ángel Isidro

Hidrobiológica

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Dez 2018, Volume 28 Nº 3 Páginas 287 - 294

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Background: Lectins act in plants and animals as recognition units against invasive pathogens, participate by activating the immune system, and function as recognition mediators of immune response in invertebrates. Few studies have evaluated the effect of immunostimulants on the activation of lectins in crustaceans. Goals: To evaluate the lectin concentration in juvenile shrimp treated with commercial and microbial immunostimulants. Methods: Five treatments were added directly to the culture tanks every third day during 12 days: 1) laminarin (0.5 mg ml-1); 2) mix 1 (Bacillus tequilensis and B. licheniformis; 2x106 CFU ml-1, 1:1 proportion); 3) mix 2 (B. endophyticus, strain YC3-B and strain C2-2; 2x106 CFU ml-1, proportion 1:1); 4) Debaryomyces hansenii (1x106 CFU ml-1); 5) control (without immunostimulants). At day 12, after the last treatments were added, samples of hemolymph were extracted from shrimp at 24, 48, and 72 h to determine lectin concentration by the ELISA method, using monoclonal antibodies against Macrobrachium rosenbergii lectin (MrL). Results: Significant differences (p <0.05) in the lectin concentration were found in shrimp groups treated with laminarin at 24 h after the last exposure to the treatment, followed by mix 1 and mix 2 at 72 h after exposure to the treatments. At 48 h, treatments did not register significant differences (p >0.05) when compared to the control groups. Shrimp exposed to D. hansenii did not show significant increase in lectin concentration compared to the control groups. Conclusions: This study showed that lectin concentration may be increased in plasma of juvenile shrimp and used as a bioindicator tool of immunostimulation.

Resumen Antecedentes: Las lectinas actúan como unidades de reconocimiento para facilitar la eliminación de patógenos invasivos en plantas y animales, participan en mecanismos de defensa y actúan como mediadores de reconocimiento en la respuesta inmune de invertebrados. Existen pocos estudios dirigidos a evaluar el efecto de inmunoestimulantes sobre la activación de lectinas en crustáceos. Objetivos: Evaluar la concentración de lectina en juveniles de camarón blanco tratados con inmunoestimulantes de origen microbiano y comercial. Métodos: Se utilizaron cinco tratamientos agregados directamente al agua de cultivo cada tercer día durante 12 días: 1) laminarina (0.5 mg ml-1); 2) mezcla 1 (Bacillus tequilensis y B. licheniformis; 2 x 106 UFC ml-1, proporción 1:1); 3) mezcla 2 (B. endophyticus, cepa YC3-B y cepa C2-2; 2 x 106 UFC ml-1, proporción 1:1); 4) Debaryomyces hansenii (1 x 106 UFC ml-1); 5) control (sin inmunoestimulantes). Después de los 12 días de tratamiento se tomaron muestras de hemolinfa a las 24, 48 y 72 h para la cuantificación de lectina en plasma, para ello se utilizaron anticuerpos monoclonales contra la lectina de Macrobrachium rosenbergii (MrL). Resultados: A las 24 h posteriores a la última aplicación del tratamiento, se registraron diferencias significativas (p < 0.05) en la concentración de lectina en el plasma de L. vannamei de los camarones expuestos al inmunoestimulante comercial laminarina respecto al grupo control, seguida por la mezcla 1 y la mezcla 2 a las 72 horas después de la aplicación de los tratamientos. A las 48 h no hubo diferencias significativas (p > 0.05) entre los tratamientos y el grupo control. No se encontraron diferencias significativas en los camarones expuestos a la cepa de D. hansenii respecto al grupo control. Conclusiones: Se observó que la concentración de lectina en el plasma de camarón blanco puede incrementarse por el uso de inmunoestimulantes y utilizarse como herramienta bioindicadora de inmunoestimulación.

https://doi.org/10.24275/uam/izt/dcbs/hidro/2018v28n3/campa 490 downloads

15.

DIAGNÓSTICO DE LA FORMALIZACIÓN ADMINISTRATIVA DE LAS EMPRESAS MEDIANAS DEDICADAS AL COMERCIO AL POR MENOR EN QUITO
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Ojeda Zambrano, Carla D.

; Gutiérrez Burbano, Jairo A.

; Córdova Ballesteros, Luis F.

Revista Economía y Política

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Jun 2018, Nº 27 Páginas 35 - 69

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen La formalización administrativa de una organización se concibe como la acción de legalizar jurídicamente su presencia en el mercado y también como la manera en que se encuentran declarados y evidenciados sus procesos organizacionales. El objetivo de este estudio descriptivo transversal es medir y describir el segundo tipo de formalización en las empresas medianas dedicadas al comercio al por menor (CIIU 47), en las parroquias urbanas de la ciudad de Quito en el año 2017 y si el tiempo de permanencia en el mercado incide en su formalización. Luego de aplicar la metodología a 58 gerentes de empresas medianas se obtuvo que el promedio general de formalización asciende a 81,72% correspondiente a la escala óptima, lo que indica que si bien existen aspectos internos débiles, en términos generales sus procesos son estables, además de que no existe relación entre su tiempo en el mercado y su nivel de formalización.

Abstract The administrative formalization of an organization is conceived as the legal action of legalizing its presence in the market and also as the way in which its organizational processes are declared and evidenced. The objective of this cross-sectional descriptive study is to measure and describe the second type of formalization in medium-sized retail businesses (ISIC 47) in the urban parishes of the city of Quito in 2017 and if the time spent in the market affects its formalization. After applying the methodology to 58 managers of medium-sized companies, it was obtained that the general average of formalization amounts to 81.72% corresponding to the optimal scale, which indicates that although there are weak internal aspects, in general terms their processes are stable. In addition, there is no relationship between their permanence in the market and their level of formalization.

https://doi.org/10.25097/rep.n27.2018.02

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