Over the last two decades, metabolomics has emerged as a pivotal tool in multidisciplinary research, providing invaluable insights into metabolome modulations and finding applications across various scientific domains, including medicine and agronomy. Nonetheless, the absence of standardized procedures in sample preparation, data acquisition, and documentation of data quality presents a significant challenge. This review underscores the critical importance of quality assurance and quality control (QA/QC) in untargeted metabolomics, advocating for the establishment of agreed-upon QA/QC reporting standards within the scientific community. We discuss the requisite quality controls at various stages of untargeted metabolomics studies, encompassing blank samples, pooled samples, and the utilization of external quality control samples. Methods for assessing accuracy, reproducibility, and identifying/correcting batch effects are addressed. Furthermore, emphasis is placed on standardizing the description of QA/QC data in scientific publications and repositories to foster reproducibility and transparency. We recommend the publication of QC data alongside studies in appropriate databases to facilitate data comparison and sharing among researchers, thereby enhancing the quality of untargeted metabolomics research. In summary, implementing standardized QA/QC data reporting, along with promoting best practices within the untargeted metabolomics community, is crucial for improving result quality and credibility and advancing research utilizing this powerful technique. decades domains agronomy Nonetheless preparation acquisition challenge QAQC QA (QA/QC agreedupon agreed upon community samples accuracy identifyingcorrecting identifying correcting addressed Furthermore transparency researchers summary technique

Resumo O estudo analisou a distribuição espacial e as desigualdades sociais na cobertura vacinal para COVID-19 entre crianças de 5 a 11 anos no Brasil. As coberturas vacinais foram calculadas para os municípios brasileiros e analisadas por região geográfica e decis com base no Índice de Desenvolvimento Humano (IDH-M) e expectativa de escolaridade aos 18 anos. Modelos multiníveis foram usados para determinar o coeficiente de partição da variância, e a estatística local bivariada de Moran I foi usada para avaliar a associação espacial. Os resultados mostraram diferenças significativas nas taxas de cobertura vacinal entre os municípios, com menor cobertura nas regiões Norte e Centro-Oeste. Municípios com menor IDH e anos de escolaridade esperados apresentaram menores taxas de cobertura vacinal. A análise de agrupamento bivariado identificou extensas concentrações de municípios nas regiões Norte e Nordeste com baixa cobertura vacinal e baixo desenvolvimento humano, enquanto alguns aglomerados de municípios nas regiões Sudeste e Sul com baixa cobertura localizavam-se em áreas com alto IDH-M. Essas descobertas destacam as desigualdades persistentes em nível municipal na cobertura vacinal entre crianças e a necessidade de intervenções para melhorar o acesso e a cobertura vacinal em áreas mais vulneráveis. COVID19 COVID 19 COVID-1 1 Brasil IDHM M (IDH-M variância CentroOeste. CentroOeste Centro Oeste. Oeste Centro-Oeste humano localizavamse localizavam se IDHM. M. IDH-M vulneráveis COVID1 COVID-

Abstract This study examined the spatial distribution and social inequalities in COVID-19 vaccine coverage among children aged 5-11 in Brazil. First and second dose vaccine coverage was calculated for all Brazilian municipalities and analyzed by geographic region and deciles based on human development index (HDI-M) and expected years of schooling at 18 years of age. Multilevel models were used to determine the variance partition coefficient, and bivariate local Moran’s I statistic was used to assess spatial association. Results showed significant differences in vaccine coverage rates among Brazilian municipalities, with lower coverage in the North and Midwest regions. Municipalities with lower HDI and expected years of schooling had consistently lower vaccine coverage rates. Bivariate clustering analysis identified extensive concentrations of municipalities in the Northern and Northeastern regions with low vaccine coverage and low human development, while some clusters of municipalities in the Southeast and South regions with low coverage were located in areas with high HDI-M. These findings highlight the persistent municipal-level inequalities in vaccine coverage among children in Brazil and the need for targeted interventions to improve vaccine access and coverage in underserved areas. COVID19 COVID 19 COVID-1 511 5 11 5-1 HDIM M (HDI-M 1 age coefficient Morans Moran s association HDIM. M. HDI-M municipallevel municipal level COVID1 COVID- 51 5-

Abstract Introduction: Experience with endoscopic retrograde cholangiopancreatography (ERCP) in the pediatric population is limited. Few medical centers have experts specifically trained in pediatric therapeutic endoscopy. As a result, patients are generally referred to adult endoscopists with high experience in the procedure. The aim of this study was to characterize the experience of an adult endoscopy unit with ERCP on pediatric patients, with a special focus on very young patients. Methods: We retrospectively analyzed indications, technical success rate, final clinical diagnosis, and complica-tions of ERCPs in children <18 years at our tertiary referral hospital center between January 1994 and June 2022. Results: Sixty-five ERCPs were performed on 57 children with a median age of 13 years (range 1-17 years). Eleven ERCPs were performed on 9 patients up to 5 years old. Indications for ERCP were as follows: biliary obstruction (n = 40), mainly due to choledocholithiasis, lithiasic acute pancreatitis (n = 19), recurrent pancreatitis (n = 3), stent extraction (n = 2), and post-operative biliary fistula (n = 1). The cannulation success rate was 95.1%. Therapeutic interventions were performed in 79% of ERCP. All patients were followed up as inpatients. Complications were recorded in two procedures (3.1%), and no procedure-related mortality occurred. Conclusion: In our experience, ERCP in children can be safely performed with high success rates by advanced adult-trained expert endoscopists at a high-volume center.

Resumo Introdução: Existe pouca experiência na realização de colangiopancreatografia retrógrada endoscópica (CPRE) na população pediátrica. A maioria dos centros carece de especialistas especificamente treinados em endoscopia terapêutica pediátrica, sendo os doentes geralmente referenciados para Gastroenterologistas de adultos com elevada experiência na técnica. O objectivo deste estudo foi caracterizar a experiência de um departamento de Gastrenterologia de adultos em CPRE pediátrica, com destaque particular nos doentes muito novos. Métodos: Foram analisadas retrospectivamente as indicações, sucesso técnico, diagnósticos e complicações das colan-giopancreatografias retrógradas endoscópicas (CPREs) realizadas no nosso hospital terciário em crianças <18 anos, entre Janeiro de 1994 e Junho de 2022. Resultados: Foram realizadas 65 CPREs em 57 crianças com idade mediana 13 anos (1-17 anos). Doze procedimentos foram realizados em 9 crianças com idade até 5 anos. As indicações para CPRE foram: obstrução biliar (n = 40), sobretudo devido a coledocolitíase, pancreatite aguda litiásica (n = 19), pancreatite recorrente (n = 3), extracção de prótese (n = 2) e fístula biliar pós cirurgia (n = 1). A taxa de sucesso de canulação foi 95.4%. Foram realizados procedimentos terapêuticos em 80.0% das CPREs. Todos os doentes foram vigiados em regime de internamento, tendo-se registado complicações em dois exames (3.1%). Não existiram mortes relacionadas com a técnica. Discussão/Conclusão: A CPRE pode ser realizada na população pediátrica com segurança e elevada taxa de sucesso por Gastrenterologistas de adultos com experiência na técnica, num centro com elevado volume de exames.

Abstract A previously healthy 16-year-old male with a ten-day history of proptosis and pain in the left upper eyelid and inflammatory signs of the left lower limb, without fever, was admitted to the Emergency Department. Computed tomography (CT) scan revealed an extensive intraorbital lesion and a multiloculated collection in the muscle planes of the thigh. The boy underwent surgical drainage and was started on empiric antibiotics. Methicillin-susceptible Staphylococcus aureus was isolated from blood and exudate cultures. Because the boy remained febrile, a chest CT scan was performed, which revealed bilateral cavitary lung lesions. He completed a long course of antibiotherapy. The study for potential immunodeficiency disorders was unremarkable. Most pediatric cases of community-associated Staphylococcus aureus bacteremia are associated with a localized source of infection. Diagnosis depends on isolation from blood and fluid aspirates. Treatment usually requires surgical drainage and intravenous antibiotherapy. Staphylococcus aureus bacteremia may be associated with multiple foci of infection, which should be recognized promptly.

Resumo Um adolescente do sexo masculino de 16 anos de idade, previamente saudável, foi admitido no Serviço de Urgência por proptose e dor na pálpebra superior esquerda e sinais inflamatórios no membro inferior esquerdo, sem febre, com dez dias de evolução. A tomografia computorizada (TC) revelou uma lesão expansiva intraorbitária e coleção multiloculada nos planos musculares da coxa. O rapaz foi submetido a drenagem cirúrgica e iniciou antibioterapia empírica. A hemocultura e cultura dos exsudados permitiram o isolamento de Staphylococcus aureus sensível à meticilina. Dado que o rapaz continuava febril, foi realizada TC torácica, que evidenciou lesões pulmonares cavitadas bilateralmente, tendo sido efetuado um esquema prolongado de antibioterapia. O estudo de potenciais imunodeficiências foi negativo. A maioria dos casos de bacteremia a Staphylococcus aureus na comunidade estão associados a focos infeciosos localizados. O diagnóstico requer isolamento no sangue ou exsudados. A abordagem implica drenagem cirúrgica e antibioterapia endovenosa. A bacteremia a Staphylococcus aureus pode associar-se a múltiplos focos de infeção, pelo que deve ser precocemente reconhecida.

Abstract Background: This was a 30-year retrospective cohort study that approximates closely to the natural history of cardiac tumors diagnosed in the fetus, since there was no case of pregnancy interruption Objective: To assess morbidity and mortality in the perinatal period and at long term in fetuses diagnosed with cardiac tumor. Our secondary objective was to assess the evaluating factors of perinatal and postnatal results. Methods: This was a retrospective cohort study with 74 pregnant women with an echocardiographic diagnosis of fetal cardiac tumor at two referral centers between May 1991 and November 2021. A descriptive analysis was performed, and data were expressed as absolute (n) and relative (%) frequencies, median and interquartile range. Fisher's exact test was used to evaluate the association of echocardiographic characteristics and clinical manifestations with perinatal and postnatal results. Global survival was calculated using the Kaplan-Meier method and the curves were compared by the log-rank test. The time of follow-up, calculated in months, corresponded to the time elapsed from hospital discharge to current status (survived/ censoring or death). The level of significance was set at 5% (p<0.05). Results: Rhabdomyoma is the most common type of cardiac tumor (85%), with a high morbidity (79.3%) and overall mortality of 17.4%. The presence of fetal hydrops was a predictor of death. Conclusion: The presence of fetal hydrops had an impact on mortality, and hence is an important factor in counselling and determining the prognosis. Most deaths occurred before hospital discharge. Background 30year year 30 fetus Objective results Methods 7 199 2021 performed n (n % (% frequencies range Fishers Fisher s KaplanMeier Kaplan Meier logrank log rank followup, followup follow up, up follow-up months survived/ survived (survived death . death) 5 p<0.05. p005 p p<0.05 0 05 (p<0.05) Results 85%, 85 85% , (85%) 79.3% 793 79 3 (79.3% 174 17 4 17.4% Conclusion prognosis 19 202 ( p00 p<0.0 (p<0.05 8 (85% 79.3 (79.3 1 17.4 20 p0 p<0. (p<0.0 (85 79. (79. 17. 2 p<0 (p<0. (8 (79 p< (p<0 (7 (p< (p

Resumo Fundamento: Seguimento de coorte retrospectiva de 30 anos que se aproxima da história natural dos tumores cardíacos diagnosticados no feto uma vez que nenhum caso foi submetido à interrupção da gestação. Objetivo: Avaliar a morbidade e mortalidade perinatal e em longo prazo em fetos com diagnóstico de tumor cardíaco. Como objetivo secundário avaliar os fatores que influenciaram os resultados perinatais e pós-natais. Método: Estudo de coorte retrospectiva envolvendo 74 gestantes com diagnóstico ecocardiográfico fetal de tumor cardíaco acompanhadas em dois serviços de referência no período de maio de 1991 a novembro de 2021. Foi realizada análise descritiva dos dados por meio de frequências absolutas (n) e relativas (%), mediana e intervalos interquartis. Para avaliar a associação entre as características ecocardiográficas e as manifestações clínicas com os resultados perinatais e pós-natais, foi aplicado o teste exato de Fisher. O cálculo da sobrevida global foi realizado pelo método de Kaplan-Meier e a comparação de curvas pelo teste de log-rank. O tempo de seguimento, calculado em meses, foi definido a partir da data de alta do hospital à data do status atual (vivo/censura ou óbito). O nível de significância considerado foi de 5% (p<0,05). Resultados: o rabdomioma é o tipo mais frequente (85%) de tumor cardíaco; apresenta alta morbidade (79,3%) e mortalidade geral de 17,4%; a presença de hidropisia fetal preditiva de óbito. Conclusão: A presença de hidropisia fetal teve impacto na mortalidade, sendo fator importante para aconselhamento e estabelecimento de prognóstico. A maioria dos óbitos ocorrem antes da alta hospitalar. Fundamento 3 gestação Objetivo pósnatais. pósnatais pós natais. natais pós-natais Método 7 199 2021 n (n %, % , (%) interquartis pósnatais, natais, Fisher KaplanMeier Kaplan Meier logrank. logrank log rank. rank log-rank seguimento meses vivo/censura vivocensura vivo censura óbito . óbito) 5 p<0,05. p005 p p<0,05 0 05 (p<0,05) Resultados 85% 85 (85% 79,3% 793 79 (79,3% 17,4% 174 17 4 Conclusão prognóstico hospitalar 19 202 (% p00 p<0,0 (p<0,05 8 (85 79,3 (79,3 17,4 1 20 ( p0 p<0, (p<0,0 (8 79, (79, 17, 2 p<0 (p<0, (79 p< (p<0 (7 (p< (p

SUMMARY OBJECTIVE: The aim of this study was to evaluate the prevalence of early neonatal sepsis in pregnant women with a positive culture for group B beta-hemolytic Streptococcus in a middle-income city in Southeastern Brazil. METHODS: A retrospective cohort study was conducted, involving singleton low- and high-risk pregnancies in whom group B beta-hemolytic Streptococcus cultures were evaluated between 35 and 37 weeks of gestation using vaginal and anal swabs. A specific medium (Todd-Hewitt) was used for culturing. The pregnant women were divided into two groups based on positive (n==201) and negative (n==420) cultures for group B beta-hemolytic Streptococcus. RESULTS: The maternal colonization rate by group B beta-hemolytic Streptococcus was 32.3%. The prevalence of early neonatal sepsis was 1.0% (2/201) among patients with a positive group B beta-hemolytic Streptococcus culture and 1.9% (8/420) among patients with a negative culture. Among the patients who underwent adequate prophylaxis, crystalline penicillin G was used in 51.9% (54/104), followed by cefazolin in 43.3% (45/104), ampicillin in 3.8% (4/104), and clindamycin in 1.0% (1/104). A model that included prematurity (p==0.001) proved to be an independent risk predictor of early neonatal sepsis [χ2 (1)==15.0, odds ratio: 16.9, 95% confidence interval: 4.7–61.6, p<0.001, Nagelkerke R2==0.157]. CONCLUSION: The prevalence of a positive culture for group B beta-hemolytic Streptococcus was high. However, the prevalence of early neonatal sepsis was low in pregnant women with both positive and negative group B beta-hemolytic Streptococcus cultures and in pregnant women with a positive culture who underwent both adequate and inadequate antibiotic prophylaxis. Prematurity proved to be an independent predictor of early neonatal sepsis, considering the entire study population.

ABSTRACT: This study compared cardiovascular and respiratory effects of dexmedetomidine and xylazine in total intravenous anesthesia with propofol, ketamine, and lidocaine. Twenty-one female dogs were submitted to ovariohysterectomy, premedicated with acepromazine and anesthetized with propofol at a variable rate. The dogs were intubated and supplemented with 100% oxygen in a circuit without rebreathing gases in spontaneous ventilation. They were divided into three groups (n=21) after induction: control (CON) with ketamine (2 mg/kg + 0.6 mg/kg/h) and lidocaine (2 mg/kg + 3 mg/kg/h), DEX and XIL with the same drugs as CON, associated with dexmedetomidine (2 µg/kg + 1 µg/kg/h) or xylazine (0.2 mg/kg + 0.1 mg/kg/h). Propofol consumption, fentanyl analgesic rescue, and cardiorespiratory and blood gas parameters were evaluated during anesthesia. The DEX group had a lower consumption of propofol (0.16 ± 0.09 mg/kg/min) compared to CON (0.24 ± 0.09 mg/kg/min), both not differing from XIL (0.23 ± 0.09 mg/kg/min). The mean arterial pressure was higher after the initial bolus in DEX (107 ± 8 mmHg) and XIL (96 ± 11 mmHg) compared to the CON group (80 ± 10 mmHg). Higher accumulation of arterial carbon dioxide and a decrease in pH were observed in the CON group. The total number of fentanyl rescues did not differ between DEX (7) and XIL (6) and were lower than CON (16). Therefore, dexmedetomidine and xylazine reduced intraoperative fentanyl consumption compared to ketamine and lidocaine infusion alone. However, only dexmedetomidine promoted lower propofol consumption and higher blood pressure values. ABSTRACT Twentyone Twenty one ovariohysterectomy rate 100 ventilation n=21 n21 n 21 (n=21 induction (CON 2 ( mgkg mg kg 06 0 6 0. mg/kg/h mgkgh h mg/kg/h, , µgkg µg µg/kg/h µgkgh 0.2 02 (0. 01 mg/kg/h. . rescue 0.16 016 16 (0.1 009 09 0.0 mg/kg/min mgkgmin min 0.24 024 24 mg/kg/min, 0.23 023 23 mg/kg/min. 107 (10 mmHg 96 (9 80 (8 mmHg. 7 (7 (6 16. (16) Therefore alone However values n=2 n2 (n=2 (0 00 (1 9 (16 n= (n= (n

RESUMO: Esse estudo comparou efeitos cardiovasculares e respiratórios da dexmedetomidina e xilazina na anestesia total intravenosa com propofol, cetamina e lidocaína. Foram submetidas a ovário-histerectomia 21 cadelas, pré-medicadas com acepromazina, anestesiadas com propofol em taxa variável. As cadelas foram intubadas e suplementadas com oxigênio 100% em circuito sem reinalação de gases em ventilação espontânea. Após a indução, foram distribuídas em três grupos (n=21): controle (CON) com cetamina (2 mg/kg + 0,6 mg/kg/h) e lidocaína (2 mg/kg + 3 mg/kg/h), DEX e XIL com os mesmos fármacos do CON, associados à dexmedetomidina (2 µg/kg + 1 µg/kg/h) ou xilazina (0,2 mg/kg + 0,1 mg/kg/h). Durante a anestesia, foram avaliados o consumo de propofol, resgate analgésico de fentanil, parâmetros cardiorepiratórios e hemogasométricos. O grupo DEX apresentou menor consumo de propofol (0,16 ± 0,09 mg/kg/min) comparado ao CON (0,24 ± 0,09 mg/kg/min), ambos sem diferir de XIL (0,23 ± 0,09 mg/kg/min). A pressão arterial média foi maior após o bolus inicial em DEX (107 ± 8 mmHg) e XIL (96 ± 11 mmHg) comparadas ao grupo CON (80 ± 10 mmHg). Houve maior acúmulo de gás carbônico arterial e diminuição do pH no grupo CON. O número total de resgates com fentanil não diferiram entre DEX (7) e XIL (6), e foram inferiores ao CON (16). Conclui-se que a dexmedetomidina e xilazina reduziram o consumo de fentanil transoperatório em comparação à infusão isolada de cetamina e lidocaína. Porém, nesse estudo apenas a dexmedetomidina promoveu menor consumo de propofol e maiores valores de pressão arterial. RESUMO ováriohisterectomia ovário histerectomia 2 prémedicadas pré medicadas acepromazina variável 100 espontânea indução n=21 n21 n (n=21) (CON ( mgkg mg kg 06 0 6 0, mg/kg/h mgkgh h mg/kg/h, , µgkg µg µg/kg/h µgkgh 0,2 02 (0, 01 mg/kg/h. . hemogasométricos 0,16 016 16 (0,1 009 09 0,0 mg/kg/min mgkgmin min 0,24 024 24 mg/kg/min, 0,23 023 23 mg/kg/min. 107 (10 mmHg 96 (9 80 (8 mmHg. 7 (7 6, (6) 16. (16) Concluise Conclui se Porém n=2 n2 (n=21 (0 00 (1 9 (6 (16 n= (n=2 (n= (n

ABSTRACT: One of the greatest challenges in poultry production is maintaining intestinal mucosal barrier integrity and gut microbiota balance. Safe alternative antimicrobials that can regulate the microbial community through animal feed have been the subject of research in poultry production. This study evaluated the effect of Mentha piperita and Melaleuca alternifolia essential oils (EOs) on the gut microbiome and morphometry of broiler quails under normal feeding conditions. The gut microbiome was studied using a completely randomized design consisting of 4 treatments, namely control, bacitracin zinc, and the Eos M. piperita and M. alternifolia, with 8 repetitions and 7 quails per treatment, totaling 224 quails from 1 to 42 days old. The intestinal contents of the slaughtered quails were collected to evaluate the gut microbiome profile of their digestive tract. Gut morphometry was analyzed using a completely randomized factorial design, with four experimental rations for three intestinal sections (4x3) and five replications. The variables studied were villus surface area and height, crypt depth, villus height to cryptdepth ratio (VH:CD), villus-crypt ratio (V:C), villus width to height ratio (VW:H), and height of the intestinal epithelium and musculature. M. alternifolia (50 mg/kg of feed) in the diet of broiler quails improved gut morphometry, similar to the results obtained with bacitracin zinc. This EO also altered the gut microbiome of quails and reduced pathogenic bacterial diversity. ABSTRACT balance EOs (EOs conditions treatments control zinc M treatment 22 old tract 4x3 x (4x3 replications depth VHCD, VHCD VH CD , (VH:CD) villuscrypt VC, VC V C (V:C) VWH, VWH VW H (VW:H) musculature 50 (5 mgkg mg kg diversity 2 4x (4x (VH:CD (V:C (VW:H 5 (

RESUMO: Um dos maiores desafios na produção de aves é manter a integridade da barreira da mucosa intestinal e o equilíbrio da microbiota intestinal. Antimicrobianos alternativos seguros, que podem regular a comunidade microbiana através da alimentação animal, têm sido objeto de pesquisa na produção avícola. O objetivo deste estudo foi avaliar o efeito dos óleos essenciais (OEs) Mentha piperita e Melaleuca alternifolia no microbioma intestinal e na morfometria de codornas de corte em condições normais de alimentação. O microbioma intestinal foi estudado em delineamento inteiramente casualizado com 4 tratamentos: controle, bacitracina zinco e os OEs M. piperita e M. alternifolia, com 8 repetições, por tratamento,com sete codornas totalizando 224 aves no período de um a 42 dias. O conteúdo intestinal das codornas abatidas foi coletado para avaliar o perfil do microbioma intestinal de seu trato digestivo. A morfometria intestinal foi estudada usando um delineamento fatorial inteiramente casualizado, consistindo de quatro rações experimentais para três secções intestinais (4x3), com cinco repetições. As variáveis estudadas foram área das vilosidades intestinais, altura das vilosidades intestinais, profundidade da cripta intestinal, relação entre a altura das vilosidades e a profundidade das criptas, relação cripta e vilosidades intestinais, relação largura e altura das vilosidades intestinais, altura do epitélio intestinal e altura da musculatura intestinal. A utilização do OE M. alternifolia (50 mg/kg de ração) na dieta de codornas de corte resultou em melhora da morfometria intestinal, semelhante aos resultados obtidos com bacitracina zinco. Além disso, alterou o microbioma intestinal de codornas e reduziu a diversidade bacteriana patogênica. RESUMO seguros animal avícola (OEs tratamentos controle M repetições tratamentocom tratamento 22 dias digestivo 4x3, 4x3 x , (4x3) criptas 50 (5 mgkg mg kg ração disso patogênica 2 4x (4x3 5 ( (4x

ABSTRACT The COVID-19 pandemic generated a large volume of scientific productions with different quality levels. The speed with which knowledge was produced and shared worldwide imposed on health management the challenge of seeking ways to identify the best available evidence to support its decisions. In response to this challenge, the Department of Science and Technology of the Brazilian Ministry of Health started offering a service to produce and provide scientific knowledge addressing priority public health issues in the pandemic scenario. Drug treatments, non-pharmacological measures, testing, reinfection and immunological response, immunization, pathophysiology, post-COVID syndrome and adverse events are among the topics covered. In this article, we discuss the strengths and lessons learned, as well as the challenges and perspectives that present a real example of how to offer the best scientific evidence in a timely manner in order to assist the decision-making process during a public health emergency. COVID19 COVID 19 COVID-1 levels decisions scenario treatments nonpharmacological non pharmacological measures testing immunization pathophysiology postCOVID post covered article learned decisionmaking decision making emergency COVID1 1 COVID-

RESUMO A pandemia de covid-19 gerou um numeroso volume de produções científicas com diferentes níveis de qualidade. A velocidade com que o conhecimento era produzido e compartilhado a nível mundial impôs à gestão em saúde o desafio de buscar meios de identificar as melhores evidências disponíveis para subsidiar suas decisões. Em resposta a este desafio, o Departamento de Ciência e Tecnologia do Ministério da Saúde do Brasil estabeleceu um serviço para elaborar e disponibilizar conhecimento científico abordando questões prioritárias de saúde pública no cenário da pandemia. Entre os temas abordados estão tratamentos medicamentosos, medidas não farmacológicas, testagem, reinfecção e resposta imunológica, imunização, fisiopatologia, síndrome pós-covid e eventos adversos. Neste artigo, discute-se os pontos fortes e lições aprendidas, bem como os desafios e perspectivas que fornecem um exemplo real sobre como disponibilizar as melhores evidências científicas, em tempo hábil e de forma oportuna, para auxiliar o processo decisório durante uma emergência em saúde pública. covid19 covid 19 covid-1 qualidade decisões medicamentosos farmacológicas testagem imunológica imunização fisiopatologia póscovid pós adversos artigo discutese discute se aprendidas oportuna covid1 1 covid-

Abstract Purpose: This study aimed to analyze the influence of human values on impulse buying behavior and whether this influence differs between genders. Originality/value: The research contributes to the understanding of the relationship between the values individuals prioritize and their impulsive buying behavior. The results can offer guidance to professionals and business managers in conducting their sales strategies and to researchers when considering human values in investigations of buying behavior, enabling new understandings. Design/methodology/approach: A model with two scales, impulsive buying and human values, consolidated in the Brazilian context, was applied, resulting in 414 responses analyzed through structural equation modeling. Findings: The results demonstrated that human values affect impulsive buying behavior, indicating that the values of pleasure, emotion, and religiosity have a positive relationship, and the values of health and knowledge have a negative relationship. Samples by gender presented significant differences, as survival and emotion have a greater positive influence on impulsive buying in men, while women are more influenced by the pleasure and emotion values and have a negative relationship with the affectivity value. Purpose genders Originality/value Originalityvalue Originality value understandings Design/methodology/approach Designmethodologyapproach Design methodology approach scales context applied 41 modeling Findings differences men 4

Resumo Objetivo: O objetivo deste estudo foi analisar a influência dos valores humanos no comportamento de compra por impulso e a diferença entre gêneros. Originalidade/valor: A pesquisa contribui para a compreensão da relação entre os valores priorizados pelo indivíduo e seu comportamento de compra impulsiva. Os resultados podem orientar profissionais e gestores de negócios na condução de suas estratégias de vendas e orientar pesquisadores a considerar os valores humanos em investigações de comportamento de compra, o que possibilita novas compreensões. Design/metodologia/abordagem: Um modelo com duas escalas, de compra impulsiva e de valores humanos, consolidadas no contexto brasileiro, foi aplicado resultando em 414 respostas, que foram analisadas por meio de modelagem de equações estruturais. Resultados: Os resultados demonstram que os valores humanos impactam no comportamento de compra impulsiva, indicando que os valores prazer, emoção e religiosidade têm relação positiva, e os valores saúde e conhecimento têm relação negativa. Amostras por gênero evidenciaram diferenças importantes, já que sobrevivência e emoção apresentaram maior influência positiva na compra impulsiva em homens; e as mulheres são mais influenciadas pelos valores prazer e emoção e apresentam relação negativa quanto ao valor afetividade. Objetivo gêneros Originalidade/valor Originalidadevalor Originalidade compreensões Design/metodologia/abordagem Designmetodologiaabordagem Design metodologia abordagem escalas brasileiro 41 respostas estruturais Resultados importantes homens afetividade 4

Resumen Este artículo presenta un análisis institucional comparado de la implementación del padrón Clima, Comunidad y Biodiversidad de Verra, para un Proyecto de REDD+ compensado en la Amazonia brasileña y los resultados derivados de la justicia ambiental (JA). A partir del Marco de Análisis y Desarrollo Institucional, el artículo identifica la interacción entre arenas, registros formales e información de los resultados de JA siguiendo el estudio de caso de un Proyecto de compensación REDD+. El artigo aponta para la importancia del ámbito operacional y del trabajo institucional de los actores locales en los resultados de los Proyectos de REDD+ compensados en cuanto a los aspectos de JA. Clima Verra REDD . (JA) Institucional arenas (JA

Abstract This article presents a comparative institutional analysis of the implementation of the Climate, Community and Biodiversity Standard, of Verra, for a REDD+ offset Project in the Brazilian Amazon and the results from Environmental Justice (EJ). Based on the Institutional Analysis and Development Framework, the article identifies interactions between political arenas and formal and informal rules in the results of EJ following the case study of a REDD+ offset Project. The article points to the importance of the operational local arena and the institutional work of local players in the results of REDD+ offset Projects regarding EJ aspects. Climate Standard Verra REDD EJ. . (EJ) Framework aspects (EJ

Resumo Este artigo apresenta a análise institucional comparada na implementação do padrão Clima, Comunidade e Biodiversidade, da Verra, para um Projeto de REDD+ offset na Amazônia brasileira e os resultados derivados da justiça ambiental (JA). A partir do Institutional Analysis and Development Framework, o artigo identifica interação entre arenas e regras formais e informais nos resultados da JA seguindo o estudo de caso de um Projeto de REDD+ offset. O artigo aponta para a importância da arena operacional e do trabalho institucional dos atores locais nos resultados dos Projetos de REDD+ offset quanto aos aspectos da JA. Clima Biodiversidade Verra REDD . (JA) Framework (JA

Thiophosphonates, a class of the organophosphate compounds, are of pharmaceutical and agricultural significance. Albeit their broad scope for peaceful uses, they may also be precursors and markers to toxic chemicals, such as the nerve agent VX and other V-series agents, leading to their inclusion into the Annex on Chemicals of the Chemical Weapons Convention (CWC). Considering that they are related to chemical warfare agents and the position of our laboratory as an Organization for the Prohibition of Chemical Weapons (OPCW) Designated Laboratory for analysis of authentic environmental samples, this study aims to contribute to the understanding of the synthesis of thiophosphonates through O,O ’-diethyl methylphosphonate conversion to O,O ’-diethyl methylphosphonothioate using Lawesson’s reagent, exploring different conditions previously reported by the literature, in order to further build a compound library for CWC verification purposes. Synthesis of the compounds has been carried out in sealed, pressure tubes, adding novelty to the present study. Reactions were followed by gas chromatography and 31P nuclear magnetic resonance, and principal component analysis was employed to understand the intricate factors influencing thiophosphonate synthesis yield as duration, temperature and solvent. The research aims to reinforce or challenge existing scientific knowledge, providing insights into parameters related to phosphonate thionation using Lawesson’s reagent, contributing to a deeper understanding of this chemical reaction. Thiophosphonates significance uses chemicals Vseries V series CWC. . (CWC) OPCW (OPCW samples OO O diethyl Lawessons Lawesson s reagent literature purposes sealed tubes P resonance duration solvent knowledge reaction (CWC

SUMMARY OBJECTIVE: The aim of this study was to compare the obstetric and perinatal complications in women who became pregnant with autologous oocytes and those who received donated oocytes (DO) in intracytoplasmic sperm injection cycles (ICSI). METHODS: A retrospective cohort study was carried out by collecting data from medical records between 2019 and 2022. Only patients who underwent ICSI in an induced cycle using their own or freshly DO, with male infertility factor and tubal factor, were included. RESULTS: A total of 120 patients were assessed, comprising 51 cases utilizing their own oocytes (control group) and 69 cases employing DO (study group). Patients receiving DO (n=69) exhibited a significantly higher mean age compared to those utilizing their own oocytes (n=51) (41.96±2.16 vs 38.54±1.42 years, p<0.001). There was no significant association between the source of oocytes and gestational age at delivery (p=0.296), birth weight (p=0.836), admission to neonatal intensive care unit (ICU) (p=0.120), or maternal admission to adult ICU (p=0.767). Additionally, the origin of oocytes did not demonstrate any significant association with the risk of pre-eclampsia (p=0.357), gestational diabetes mellitus (p=0.187), premature rupture of membranes (p=0.996), uterine atony (p=0.996), placenta previa (p=0.393), oligohydramnios (p=0.393), or gestational hypertension (p=0.393)." CONCLUSION: An increase in obstetric and perinatal complications was not observed in pregnancies with DO compared to pregnancies with autologous oocytes in women undergoing ICSI without prior comorbidities. Further studies with larger sample sizes are required to validate our findings. OBJECTIVE (DO ICSI. . (ICSI) METHODS 201 2022 included RESULTS 12 assessed 5 control group 6 group. n=69 n69 n (n=69 n=51 n51 (n=51 41.96±2.16 4196216 41 96 2 16 (41.96±2.1 3854142 38 54 1 42 38.54±1.4 years p<0.001. p0001 p p<0.001 0 001 p<0.001) p=0.296, p0296 p=0.296 , 296 (p=0.296) p=0.836, p0836 p=0.836 836 (p=0.836) (ICU p=0.120, p0120 p=0.120 (p=0.120) p=0.767. p0767 p=0.767 767 (p=0.767) Additionally preeclampsia pre eclampsia p=0.357, p0357 p=0.357 357 (p=0.357) p=0.187, p0187 p=0.187 187 (p=0.187) p=0.996, p0996 p=0.996 996 (p=0.996) p=0.393, p0393 p=0.393 393 (p=0.393) p=0.393. (p=0.393). CONCLUSION comorbidities findings (ICSI 20 202 n=6 n6 (n=6 n=5 n5 (n=5 41.96±2.1 419621 4 9 (41.96±2. 385414 3 38.54±1. p000 p<0.00 00 p029 p=0.29 29 (p=0.296 p083 p=0.83 83 (p=0.836 p012 p=0.12 (p=0.120 p076 p=0.76 76 (p=0.767 p035 p=0.35 35 (p=0.357 p018 p=0.18 18 (p=0.187 p099 p=0.99 99 (p=0.996 p039 p=0.39 39 (p=0.393 n= (n= 41.96±2. 41962 (41.96±2 38541 38.54±1 p00 p<0.0 p02 p=0.2 (p=0.29 p08 p=0.8 8 (p=0.83 p01 p=0.1 (p=0.12 p07 p=0.7 7 (p=0.76 p03 p=0.3 (p=0.35 (p=0.18 p09 p=0.9 (p=0.99 (p=0.39 (n 41.96±2 4196 (41.96± 3854 38.54± p0 p<0. p=0. (p=0.2 (p=0.8 (p=0.1 (p=0.7 (p=0.3 (p=0.9 41.96± 419 (41.96 385 38.54 p<0 p=0 (p=0. 41.96 (41.9 38.5 p< p= (p=0 41.9 (41. 38. (p= 41. (41 (p (4 (

ABSTRACT Background: Visceral leishmaniasis (VL) is a public health problem and is a relevant cause of death in developing countries. This study aimed to evaluate the 20-year survival and predictors of worse prognosis in patients with VL admitted to a reference hospital for the treatment of infectious diseases between 1995 and 2016 in northern Minas Gerais, an area of high endemicity for VL. Methods: This retrospective cohort study was conducted at a hospital in northern Minas Gerais, Brazil. All patients with VL were evaluated over a 20-year period. The medical records were thoroughly analyzed. Cox regression analysis was performed to estimate factors associated with the probability of survival. Results: The cohort included 972 individuals, mostly male children <10 years old, from urban areas who presented at admission with the classic triad of fever, hepatosplenomegaly, and skin pallor. The mean hemoglobin level was 7.53 mg/dl. The mean interval between symptom onset and hospital admission was 40 days. The instituted therapies ranged from pentavalent antimonates to amphotericin, or both. The probability of survival was reduced to 78% one year after symptom onset. Hemoglobin levels and age were strongly associated with the probability of survival. Conclusions: Regardless of the mechanism underlying the reduction in hemoglobin and the non-modifiable factors of age, early initiation of drug treatment is the most appropriate strategy for increasing survival in patients with VL, which challenges health systems to reduce the interval between the onset of symptoms and hospital admission. Background (VL countries 20year 20 199 201 Gerais Methods Brazil period analyzed Results 97 individuals 10 <1 old fever hepatosplenomegaly pallor 753 7 53 7.5 mgdl mg dl mg/dl 4 days amphotericin both 78 Conclusions nonmodifiable non modifiable 2 19 9 1 < 75 5 7.

Abstract Finding ways to reuse residue is an alternative not only to reduce its presence in urban and natural environments, but also to reduce costs and consumption of resources by industries. The objective of this paper is to evaluate the properties of concretes made by replacing conventional coarse and fine aggregates with marble residue. Morphological, granulometry, specific gravimetry and crystalline phase analyses were carried out in the aggregates. The composition of the constituents, by mass, was 1:1,2:3 of Portland cement CPIII-32 RS, fine aggregates, and coarse aggregate, respectively, with the addition of 0.4 of water, molded and cured in water. About 25 concrete samples were made, varying the amount of coarse/fine aggregate in the cement mass according to the aggregates proportions of: A= 100% gravel/ 100% sand, B= 100% coarse RRO/ 50% fine RRO + 50% sand, C= 50% coarse RRO + 50% gravel/ 100% fine RRO, and D = 100% coarse RRO/100% fine RRO. The cylindrical samples measuring ∅50 x 100 mm were subjected to water absorption and compressive strength tests, and the results were compared with standards and literature. The water absorption was smaller than 2.5% and the compressive strength values were between 27-33 MPa, close to the resistance of cement used. Composition B presented the best results among the others, indicating the possibility of using the residue from marble for production of concretes for various purposes such as low load paving, sidewalks, blocks, among others. However, for interlocking floors, higher performance cement should be used to achieve the required strength. This type of concrete adds value to the residue and mitigates environmental impacts. environments industries Morphological granulometry constituents 11,23 1123 1 1,2 3 2 1:1,2: CPIII32 CPIII 32 CPIII-3 RS respectively 04 0 4 0. coarsefine A gravel sand 50 C RRO100 RRO/100 ∅5 10 tests literature 5 2.5 2733 27 33 27-3 MPa others paving sidewalks blocks However floors impacts 11 23 11,2 112 12 1, 1:1,2 CPIII3 CPIII- RRO10 RRO/10 ∅ 2. 273 27- 11, 1:1, RRO1 RRO/1 1:1 1:

Resumo Encontrar formas de reaproveitar resíduos é uma alternativa não só para reduzir sua presença em ambientes urbanos e naturais, mas também para reduzir custos e consumo de matérias primas pelas indústrias. O objetivo deste trabalho é avaliar as propriedades de concretos fabricados através da substituição de agregados graúdos e miúdos convencionais por resíduo de mármore. Foram realizadas análises de morfologia, granulometria, massa específica e da fase cristalina dos agregados. A composição dos constituintes, em massa, foi 1:1,2:3 de cimento Portland CPIII-32 RS, agregados miúdos e agregados graúdos, respectivamente, com adição de 0,4 de água, moldados e curados em água. Foram confeccionadas cerca de 25 amostras de concreto, variando a quantidade de agregado graúdo/fino na massa de cimento de acordo com as proporções de agregados de: A= 100% brita/ 100% areia, B= 100% RRO grosso/ 50% RRO fino + 50% areia, C= 50% RRO grosso + 50% cascalho/ 100% RRO fino, e D = 100% RRO grosso/100% RRO fino. As amostras cilíndricas medindo ∅50 x 100 mm foram submetidas a testes de absorção de água e resistência à compressão, e os resultados obtidos foram comparados com algumas normas e com a literatura. A absorção de água foi inferior a 2,5% e a resistência à compressão ficou entre 27-33 MPa, valor próximo à resistência do cimento utilizado. A composição B apresenta os melhores resultados entre as demais, indicando a possibilidade de utilização do resíduo do mármore para produção de concretos para diversas finalidades como pavimentação de baixa carga, calçadas, blocos, entre outros. No entanto, para pisos intertravados, deve-se utilizar cimento de maior desempenho para atingir a resistência necessária. Esse tipo de concreto agrega valor ao resíduo e mitiga os impactos ambientais. naturais indústrias morfologia granulometria constituintes 11,23 1123 1 1,2 3 2 1:1,2: CPIII32 CPIII 32 CPIII-3 RS respectivamente 04 0 4 0, graúdofino graúdo brita areia 50 C cascalho grosso100 grosso/100 ∅5 10 literatura 5 2,5 2733 27 33 27-3 MPa utilizado demais carga calçadas blocos outros entanto intertravados devese deve se necessária ambientais 11 23 11,2 112 12 1, 1:1,2 CPIII3 CPIII- grosso10 grosso/10 ∅ 2, 273 27- 11, 1:1, grosso1 grosso/1 1:1 1: