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ABSTRACT Objective: The aim of this cross-sectional study was to estimate the prevalence of XLH in Paraná, a state in southern Brazil, and report the clinical features and complications of the disease. Materials and methods: We invited all endocrinologists (n = 205), nephrologists (n = 221), orthopedic surgeons (n = 1020), and pediatricians (n = 1000) in Paraná to fill out an electronic survey with information on patients with X-linked hypophosphatemia (XLH), and searched the records of the state's health department for all calcitriol prescriptions in 2018. Results: In all, 244 (10%) specialists responded to the email, of whom 18 (7.4%) reported to be taking care of patients with XLH and answered the online survey. A total of 57 patients with XLH were identified (prevalence 5 per million inhabitants). The median age at diagnosis was 22 years, and 42.2% were children and adolescents. Fifteen patients had genetic testing showing a PHEX mutation. Overall, 91.2% had bone deformities, 30.8% had a history of fragility fractures, and 22.4% had renal complications. Conclusion: This study demonstrated a prevalence of XLH of 5 cases per million inhabitants in the state of Paraná, a rate lower than the one reported in other countries. Manifestations of renal calcification and bone fragility were frequent among the patients. This is the first epidemiological study evaluating the prevalence and clinical presentation of XLH in Latin America.

https://doi.org/10.20945/2359-3997000000296 578 downloads

Beyond diversity loss and climate change: Impacts of Amazon deforestation on infectious diseases and public health
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ELLWANGER, JOEL HENRIQUE

; KULMANN-LEAL, BRUNA

; KAMINSKI, VALÉRIA L.

; VALVERDE-VILLEGAS, JACQUELINE MARÍA

; VEIGA, ANA BEATRIZ G. DA

; SPILKI, FERNANDO R.

; FEARNSIDE, PHILIP M.

; CAESAR, LÍLIAN

; GIATTI, LEANDRO LUIZ

; WALLAU, GABRIEL L.

; ALMEIDA, SABRINA E.M.

; BORBA, MAURO R.

; HORA, VANUSA P. DA

; CHIES, JOSÉ ARTUR B.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 Nº 1 elocation e20191375

Resumo: En | Texto: En | PDF: En

Abstract Amazonian biodiversity is increasingly threatened due to the weakening of policies for combating deforestation, especially in Brazil. Loss of animal and plant species, many not yet known to science, is just one among many negative consequences of Amazon deforestation. Deforestation affects indigenous communities, riverside as well as urban populations, and even planetary health. Amazonia has a prominent role in regulating the Earth’s climate, with forest loss contributing to rising regional and global temperatures and intensification of extreme weather events. These climatic conditions are important drivers of emerging infectious diseases, and activities associated with deforestation contribute to the spread of disease vectors. This review presents the main impacts of Amazon deforestation on infectious-disease dynamics and public health from a One Health perspective. Because Brazil holds the largest area of Amazon rainforest, emphasis is given to the Brazilian scenario. Finally, potential solutions to mitigate deforestation and emerging infectious diseases are presented from the perspectives of researchers in different fields.

https://doi.org/10.1590/0001-3765202020191375 16314 downloads

Molecular Diagnostics of Dengue by Reverse Transcription-Loop Mediated Isothermal Amplification (RT-LAMP) in Disposable Polyester-Toner Microdevices
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Mendes, Geovana M.

; Oliveira, Kézia G.

; Borba, Juliane C.

; Oliveira, Thais S.

; Fiaccadori, Fabíola S.

; Nogueira, Maurício L.

; Bailão, Alexandre M.

; Soares, Célia Maria A.

; Carrilho, Emanuel

; Duarte, Gabriela R. M.

Journal of the Brazilian Chemical Society

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Set 2019, Volume 30 Nº 9 Páginas 1841 - 1849

Resumo: En | Texto: En | PDF: En

Dengue is one of the most prevalent infectious tropical diseases in the world, with high incidence in over 100 countries. Rapid and reliable diagnosis of dengue is of great importance for public health. To simplify molecular diagnostics, isothermal amplification techniques have recently emerged as an alternative to conventional methods of deoxyribonucleic acid (DNA) amplification. Here, we developed a one-step method for dengue virus detection from real sample based on RT-LAMP (reverse transcription-loop mediated isothermal amplification) in a disposable microdevice. The reaction was thermally controlled with a thermoblock for 15 min at 72 ºC. At the end of the incubation time, we either removed the solution for detection of fragments by gel electrophoresis or added DNA intercalator for visual detection on-chip. Our results demonstrated that it is possible to detect dengue virus through RT-LAMP directly from a serum sample, without previous ribonucleic acid (RNA) extraction. The success of RT-LAMP was confirmed in reactions initiated with 0.8 fg µL-1 of RNA, which represents 200 copies of RNA per µL. RT-LAMP in a polyester-toner (PeT) microdevice is a simple and inexpensive method that allows for rapid detection of dengue virus with high reliability and great potential for point-of-care applications.

https://doi.org/10.21577/0103-5053.20190092 1872 downloads

Brazilian recommendations on the safety and effectiveness of the yellow fever vaccination in patients with chronic immune-mediated inflammatory diseases
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Pileggi, Gecilmara Salviato

; Mota, Licia Maria Henrique Da

; Kakehasi, Adriana Maria

; Souza, Alexandre Wagner De

; Rocha, Aline

; Melo, Ana Karla Guedes de

; Fonte, Caroline Araujo M. da

; Bortoletto, Cecilia

; Brenol, Claiton Viegas

; Marques, Claudia Diniz Lopes

; Zaltman, Cyrla

; Borba, Eduardo Ferreira

; Reis, Enio Ribeiro

; Freire, Eutilia Andrade Medeiros

; Klumb, Evandro Mendes

; Christopoulos, Georges Basile

; Laurindo, Ieda Maria M.

; Ballalai, Isabella

; Costa, Izaias Pereira Da

; Michelin, Lessandra

; Valadares, Lilian David de Azevêdo

; Chebli, Liliana Andrade

; Lacerda, Marcus

; Toscano, Maria Amazile Ferreira

; Yazbek, Michel Alexandre

; Vieira, Rejane Maria R. De Abreu

; Magalhães, Renata

; Kfouri, Renato

; Richtmann, Rosana

; Merenlender, Selma Da Costa Silva

; Valim, Valeria

; Assis, Marcos Renato De

; Kowalski, Sergio Candido

; Trevisani, Virginia Fernandes Moça

Advances in Rheumatology

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2019, Volume 59 elocation 17

Resumo: En | Texto: En | PDF: En

Abstract Background: In Brazil, we are facing an alarming epidemic scenario of Yellow fever (YF), which is reaching the most populous areas of the country in unvaccinated people. Vaccination is the only effective tool to prevent YF. In special situations, such as patients with chronic immune-mediated inflammatory diseases (CIMID), undergoing immunosuppressive therapy, as a higher risk of severe adverse events may occur, assessment of the risk-benefit ratio of the yellow fever vaccine (YFV) should be performed on an individual level. Main body of the abstract: Faced with the scarcity of specific orientation on YFV for this special group of patients, the Brazilian Rheumatology Society (BRS) endorsed a project aiming the development of individualized YFV recommendations for patients with CIMID, guided by questions addressed by both medical professionals and patients, followed an internationally validated methodology (GIN-McMaster Guideline Development). Firstly, a systematic review was carried out and an expert panel formed to take part of the decision process, comprising BRS clinical practitioners, as well as individuals from the Brazilian Dermatology Society (BDS), Brazilian Inflammatory Bowel Diseases Study Group (GEDIIB), and specialists on infectious diseases and vaccination (from Tropical Medicine, Infectious Diseases and Immunizations National Societies); in addition, two representatives of patient groups were included as members of the panel. When the quality of the evidence was low or there was a lack of evidence to determine the recommendations, the decisions were based on the expert opinion panel and a Delphi approach was performed. A recommendation was accepted upon achieving ≥80% agreement among the panel, including the patient representatives. As a result, eight recommendations were developed regarding the safety of YFV in patients with CIMID, considering the immunosuppression degree conferred by the treatment used. It was not possible to establish recommendations on the effectiveness of YFV in these patients as there is no consistent evidence to support these recommendations. Conclusion: This paper approaches a real need, assessed by clinicians and patient care groups, to address specific questions on the management of YFV in patients with CIMID living or traveling to YF endemic areas, involving specialists from many areas together with patients, and might have global applicability, contributing to and supporting vaccination practices. We recommended a shared decision-making approach on taking or not the YFV.

https://doi.org/10.1186/s42358-019-0056-x 1111 downloads

Comportamento mecânico e clínico de próteses monolíticas à base de zircônia: revisão de literatura
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Ottoni, R.

; Borba, M.

Cerâmica

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Dez 2018, Volume 64 Nº 372 Páginas 547 - 552

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Próteses fixas com infraestrutura de metal ou cerâmica apresentam muitas falhas devido ao lascamento da cerâmica vítrea de cobertura. Assim, próteses monolíticas produzidas com apenas um material foram desenvolvidas no intuito de diminuir estas falhas. Cerâmicas à base de zircônia tiveram suas propriedades ópticas modificadas para permitir a confecção de próteses monolíticas. Portanto, o objetivo deste estudo foi verificar através de uma revisão da literatura o comportamento mecânico e clínico das próteses monolíticas à base de zircônia. Foi feita uma busca na base de dados PubMed/Medline (2010 a 2017) utilizando as palavras-chave: zircônia (zirconia), monolítica (monolithic) e prótese (prosthesis). Foram selecionados 13 estudos laboratoriais e 5 estudos clínicos. Os estudos laboratoriais mostraram que próteses monolíticas à base de zircônia apresentam alta resistência à fratura, à fadiga e ao lascamento. Próteses com espessura mínima de 0,7 mm devem ser confeccionadas para que sejam suficientemente resistentes às cargas impostas pela mastigação, tendo carga de fratura semelhante a coroas metalocerâmicas. Os estudos clínicos relatam baixas taxas de falha para esse tratamento protético. Próteses monolíticas à base de zircônia apresentam comportamento mecânico e clínico favorável para a reabilitação da região posterior.

Abstract Dental prosthesis produced with metal or ceramic infrastructure show high rates of chipping of the glass-ceramic veneer. Thus, monolithic prosthetic restorations produced with only one material were developed in order to reduce these failures. Zirconia-based ceramics had their optical properties modified to produce monolithic restorations. Therefore, the objective of this study was to verify, through a literature review, the mechanical and clinical behavior of zirconia-based monolithic dental prostheses. PubMed/Medline database was used to search for manuscripts (2010 to 2017) using the following keywords: zirconia, monolithic and prosthesis. Thirteen in vitro studies and five clinical studies were selected. Studies have shown that zirconia-based monolithic prostheses exhibit high resistance to fracture, fatigue and chipping. To support the chewing load and achieve similar mechanical behavior as the metal-ceramic system, restorations should be produced with a minimum thickness of 0.7 mm. Clinical studies report low failure rates for this prosthetic treatment. Zirconia-based monolithic dental prostheses present mechanical and clinical behavior favorable for the rehabilitation of the posterior region.

https://doi.org/10.1590/0366-69132018643722340 2856 downloads

Efecto de los antimaláricos sobre los diferentes dominios del índice de daño SLICC en pacientes de la cohorte GLADEL
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Pons-Estel, Guillermo J.

; Quintana, Rosana

; Wojdyla, Daniel

; Alarcón, Graciela S.

; Serrano, Rosa María

; Ugarte-Gil, Manuel

; Pimentel-Quiroz, Víctor

; Soriano, Enrique R.

; Catoggio, Luis J.

; Scolnik, Marina

; Sacnun, Mónica

; Saurit, Verónica

; Caeiro, Francisco

; Alvarellos, Alejandro

; Sarano, Judith

; García, Mercedes

; Onetti, Laura

; Drenkard, Cristina

; Berbotto, Guillermo

; Scherbarth, Hugo R.

; Sato, Emilia

; Bonfa, Eloisa

; Ferreira Borba, Eduardo

; Costallat, Lilian

; Xavier, Ricardo

; Tavares Brenol, Joao C.

; Da Silva, Nilzio A.

; Cavalcanti, Fernando

; Massardo, Loreto

; Jacobelli, Sergio

; Neira, Oscar

; Molina, José F

; Vásquez, Gloria

; Gómez-Puerta, José A.

; Alonso Gonzalez, Luis

; Iglesias Gamarra, Antonio

; Guibert-Toledano, Marlene

; Reyes, Gil A.

; Cardiel, Mario H.

; Pascual-Ramos, Virginia

; García de la Torre, Ignacio

; Barile, Leonor

; Silveira, Luis H.

; Amigo, Mary-Carmen

; Sauza del Pozo, María Josefina

; Acevedo-Vásquez, Eduardo M.

; Alfaro-Lozano, José

; Segami, María Inés

; Chacón-Díaz, Rosa

; Abadi, Isaac

; Esteva Spinetti, María H

; Pons-Estel, Bernardo A.

Revista argentina de reumatología

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Set 2018, Volume 29 Nº 3 Páginas 6 - 10

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Objective: To assess the effects of antimalarials (AM) over the items of the SLICC Damage Index (SDI). Methods: Patients with recent (≤2 years) diagnosis of systemic lupus erythematosus (SLE) from the GLADEL cohort were studied. End-point: increase in items SDI since cohort entry. Independent variables (socio-demographic, clinical, laboratory and treatment) were included. The effect of AM as a time dependent variable on most frequent SDI items (adjusting for potential confounders) was examined with a multivariable Cox regression model. Results: Of the 1466 patients included in this analysis, 1049 (72%) received AM with a median exposure time of 30 months (Q1-Q3: 11-57). Damage occurred in 665 (45%) patients during a median follow-up time of 24 months (Q1-Q3: 8-55). There were 301 integument, 208 renal, 149 neuropsychiatric, 98 musculoskeletal, 88 cardiovascular and 230 others less frequently represented damages. After adjusting for potential confounders at any time during follow-up, a lower risk of renal damage (HR 0.652; 95% CI: 0.472-0.901) and borderline for neuropsychiatric damage (HR 0.701, 95% CI: 0.481-1.024) was found. Conclusion: In the GLADEL cohort, after adjustment for possible confounding factors, AM were independently associated with a reduced risk of renal damage accrual.

Objetivos: Estimar el efecto de los antimaláricos (AM) sobre los diferentes dominios del índice de daño SLICC (SDI). Métodos: Se estudiaron pacientes con diagnóstico clínico reciente (≤2 años) de lupus eritematoso sistémico (LES) de la cohorte GLADEL. Variable de estudio: aumento en los dominios del SDI desde el ingreso a la cohorte. Variables independientes: características sociodemográficas, clínicas, laboratorio y tratamientos. El efecto de los AM, como variable dependiente del tiempo, sobre los dominios más frecuentes del SDI (ajustado por factores de confusión) fue examinado con un modelo de regresión de Cox multivariado. Resultados: De 1466 pacientes estudiados, 1049 (72%) recibieron AM con un tiempo medio de exposición de 30 meses (Q1-Q3: 11-57) y 665 pacientes (45%) presentaron daño durante un seguimiento medio de 24 meses (Q1-Q3: 8-55); 301 eventos fueron cutáneos, 208 renales, 149 neuropsiquiátricos, 98 musculoesqueléticos, 88 cardiovasculares y 230 otros. Después de ajustar por factores de confusión, el uso de AM se asoció a un menor riesgo de daño renal (HR 0,652; IC 95%: 0,472-0,901) y en el límite de la significancia estadística (HR 0,701, IC 95%: 0,481-1,024) para el dominio neuropsiquiátrico. Conclusión: En GLADEL, el uso de AM se asoció independientemente a un menor riesgo de daño acumulado renal.

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Hematological and biochemical indicators of maturity in foals and their relation to the placental features
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Feijó, Lorena S.

; Curcio, Bruna R.

; Pazinato, Fernanda M.

; Almeida, Bruno A.

; Moraes, Bruna S.S.

; Borba, Luciana A.

; Feijó, Josiane O.

; Nogueira, Carlos E.W.

Pesquisa Veterinária Brasileira

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Jun 2018, Volume 38 Nº 6 Páginas 1232 - 1238

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: A saúde do neonato está diretamente relacionada às condições gestacionais e eficiência placentária. Os objetivos deste estudo foram: (1) avaliar parâmetros hematológicos e bioquímicos de potros nascidos de éguas com placentite ao nascimento e com 24h de vida e (2) verificar se a patologia placentária exerceu influência no grau de maturidade através da resposta hemato-bioquímica destes neonatos. De acordo com os resultados histopatológicos placentários (controle e placentite) e grau de maturidade neonatal (maturo e imaturo), os potros foram divididos em três grupos: grupo controle (n=22); e potros nascidos de éguas com placentite classificados como (2) maturos (n=26) e (3) imaturos (n=10). Foi avaliado hematócrito e concentrações sanguíneas de fibrinogênio, proteína plasmática total, leucócitos totais, lactato, glicose, creatinina, uréia, albumina, bilirrubinas, triglicerídeos, colesterol, cálcio, fósforo, magnésio, aspartato aminotransferase (AST), creatina quinase (CK), fosfatase alcalina (FA) e gama glutamiltranferase (GGT). As características placentárias foram significativamente diferentes entre os graus de maturidade neonatal (P=0.001). Éguas com placentite aguda produziram mais potros imaturos (n=8/10; 80%). No nascimento, os potros imaturos apresentaram maiores concentrações de fibrinogênio (P=0,003), creatinina (P=0,021), colesterol total (P=0,0014), AST (P=0,001), GGT (P=0,002), bilirrubina indireta (P=0,010) e total (P=0,001) e menor concentração de albumina (P=0,002). Os potros nascidos de éguas com placentite apresentaram hiperlactatemia com 24h de vida (P=0,002). A placentite aguda exerceu influência na maturidade neonatal, permitindo uma maturação fetal acelerada, porém, incompleta. Mensurações dos níveis sanguíneos de lactato, fibrinogênio, creatinina, colesterol total, AST, GGT, bilirrubinas e albumina, associado à avaliação clínica, física e comportamental, podem contribuir como indicadores de maturidade neonatal.

ABSTRACT: Newborn’s health is directly related to gestational conditions and placental efficiency. The aims of this study were: (1) To evaluate hematological and biochemical parameters of foals born from mares with placentitis at birth and at 24h of age, (2) to verify if placental pathology had any influence on neonatal maturity degree through hematological and biochemical response of those foals. According to placental findings (control and placentitis) and neonatal maturity degree (mature and immature), foals were divided into three groups: (1) Control group (n=22), foals born from mares with placentitis and classified as (2) Mature (n=26), and (3) Immature (n=10). The hematocrit and plasma concentration of fibrinogen, total plasma protein, white blood cells count, lactate, glucose, creatinine, urea, albumin, bilirubin, triglyceride, cholesterol, calcium, phosphorus, magnesium, aspartate aminotransferase (AST), creatine kinase (CK), alkaline phosphatase (ALP), and gamma-glutamyltransferase (GGT) were measured. Placental features were significantly different between neonatal maturity degree (P=0.001). Mares that had acute placentitis foaled more immature neonates (n=8/10; 80%). Concentrations of fibrinogen (P=0.003), creatinine (P=0.021), total cholesterol (P=0.014), AST (P=0.001), GGT (P=0.002), total (P=0.001) and unconjugated bilirubin (P=0.010) were higher at birth in the Immature group, whereas albumin levels were lower (P=0.002). Foals born from mares with placentitis presented hyperlactatemia at 24h of age (P=0.002). Acute placentitis had an influence on the neonatal maturity, allowing an accelerated but incomplete fetal maturation. The monitoring of lactate, fibrinogen, creatinine, bilirubin, cholesterol, albumin, AST, and GGT levels, associated with clinical, physical, and behavior evaluation may contribute as indicators of neonatal maturity.

https://doi.org/10.1590/1678-5150-pvb-5503 1176 downloads

Ocorrência de brucelose e tuberculose bovinas no Rio Grande do Sul com base em dados secundários
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Todeschini, Bernardo

; Costa, Eduardo F.

; Santiago-Neto, Waldemir

; Santos, Diego V.

; Groff, Ana Cláudia M.

; Borba, Mauro R.

; Corbellini, Luís G.

Pesquisa Veterinária Brasileira

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Jan 2018, Volume 38 Nº 1 Páginas 15 - 22

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Avaliaram-se os resultados de testes para diagnóstico da brucelose e tuberculose bovinas objetivando analisar a distribuição de positividade e características de performance do programa de controle. A análise utilizou como dados secundários todos os resultados de testes para diagnóstico realizados em bovinos no ano de 2008 no estado do Rio Grande do Sul (RS) por médicos veterinários habilitados para atuação no âmbito do Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose Animal. Os dados foram estatisticamente ajustados para minimizar os efeitos de valores extremos e de dados faltantes. Os testes alcançaram 66,80% dos 497 municípios do RS e incluíram a participação de 165 médicos veterinários habilitados. 40,21% dos testes foram realizados nos meses de abril, setembro e outubro, sendo que em 73,90% dos estabelecimentos foram realizados testes para ambas as enfermidades. No caso da brucelose verificou-se que foram testados 35.289 animais, com uma frequência de positivos de 0,25%, enquanto a frequência de estabelecimentos positivos entre os 3.406 testados foi 0,94%. Fêmeas apresentaram frequência de positividade mais elevada (0,29%, de 26.724 testadas) do que machos (0,13%, de 5.235 testados). Animais entre 48-60 meses de idade apresentaram chance de positividade superior às demais faixas etárias (Razão de Chances (RC) =2,63; IC 95%=1,63-4,26). Animais de aptidão leiteira representaram 62,66% dos animais testados, e apresentaram maior chance de positividade do que animais de corte (RC=2,32; IC 95%=1,38-3,90). Adicionalmente, 73,74% dos estabelecimentos testados eram dedicados à produção de leite. Já no caso da tuberculose foram testados 62.149 animais distribuídos em 5.151 propriedades, com frequência de positivos de 0,87% e 3,13%, respectivamente. A chance de positividade aumentou com a idade, sendo que animais com idade acima de 48 meses de idades apresentaram valores superiores aqueles verificados em animais mais jovens (RC=2,07; IC 95%=1,73-2,48). 59,74% dos animais testados eram de aptidão leiteira, os quais apresentaram mais chance de serem positivos do que aqueles de corte (RC=5,03; IC 95%=4,09-6,94). Propriedades leiteiras representaram 78,50% da totalidade dos testes para tuberculose. A análise comparativa do presente trabalho com estudos precedentes de prevalência da brucelose sugere que as ações de controle em curso têm sido efetivas na redução da prevalência da enfermidade no RS. Por outro lado, as informações obtidas no âmbito da tuberculose podem ser indicadoras da condição desta enfermidade, especialmente pela inexistência de estudos de prevalência realizados há menos de 30 anos. Adicionalmente, conclui-se que a utilização de dados secundários, desde que devidamente ajustados, pode ser uma ferramenta eficaz na gestão de programas de saúde animal e em sistemas de monitoramento e vigilância.

ABSTRACT: Results of tests for the diagnosis of bovine brucellosis and tuberculosis were evaluated aiming to analyze the distribution of positivity and characteristics of performance of the control program. The analysis used as secondary data the results of all diagnostic tests of bovines executed in 2008 in the state of Rio Grande do Sul (RS) by veterinarians authorized to operate under the National Program for Control and Eradication of Animal Brucellosis and Tuberculosis. The data were statistically adjusted to minimize the effect of outliers and missing data. The tests achieved 66.80% of the 497 municipalities in the RS and included the participation of 165 authorized veterinarians. 40.21% of tests were carried out in the months of April, September and October, and in 73.90% of establishments had tests performed for both diseases. In the case of brucellosis it appeared that 35,289 animals were tested, with positivity of 0.25%, while the frequency of positive holdings among the 3,406 tested was 0.94%. Females had higher positivity rate (0.29% of 26,724 tested) than males (0.13% of 5,235 tested). Animals between 48-60 months of age presented higher chance of positivity compared to other age groups (Odds Ratio (OR) = 2.63; CI 95% = 1.63 - 4.26). Dairy cattle represented 62.66% of tested animals, which were more likely to appear as positive than beef animals (OR = 2.32; CI 95% = 1.38 - 3.90). In addition, 73.74% of the tested holdings were dairy ones. In the case of tuberculosis, 62,149 animals were tested, distributed in 5,151 holdings, with positivity of 0.87% and 3.13%, respectively. The chance of positivity increased with age, being that animals older than 48 months presented higher values compared to those found in younger animals (OR = 2.07; CI 95% = 1.73 - 2.48). Dairy cattle represented 59.74% of tested animals, which were more likely to be positive than beef ones (OR = 5.03; CI 95% = 4.09 - 6.94). Dairy holdings were 78.50% of all the tests for tuberculosis. The comparative analysis of this study with previous studies on brucellosis prevalence suggested that current control measures have been effective in reducing the prevalence of this disease in RS. On the other hand, information obtained on tuberculosis can be indicators of the condition of this disease, especially in the lack of prevalence studies carried out in the last 30 years. Finally, it is concluded that the use of secondary data, if properly adjusted, can be an effective tool in the management of animal health programs and monitoring and surveillance systems.

https://doi.org/10.1590/1678-5150-pvb-4712 3258 downloads

Juvenile dermatomyositis: is periodontal disease associated with dyslipidemia?
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Kozu, Kátia T.

; Silva, Clovis A.

; Aikawa, Nadia E.

; Pereira, Rosa M. R.

; Sallum, Adriana M.

; Savioli, Cynthia

; Borba, Eduardo

; Campos, Lucia M.

Advances in Rheumatology

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2018, Volume 58 elocation 28

Resumo: En | Texto: En | PDF: En

Abstract Background: Association between periodontal disease and dyslipidemia was recently reported in healthy adults. However, a systematic evaluation of concomitant periodontal diseases and lipid profile was not carried out in juvenile dermatomyositis (JDM). A cross-section study was performed in 25 JDM patients and 25 healthy controls, assessing demographic data, periodontal evaluation, fasting lipoproteins and anti-lipoprotein lipase antibodies. Disease parameters, laboratorial tests and treatment were also evaluated in JDM patients. Results: The mean current age was similar in patients and controls (11.5 ± 3.75 vs. 11.2 ± 2.58 years, p = 0.703). Regarding lipid profile, the median triglycerides [80(31–340) vs. 61(19–182)mg/dL, p = 0.011] and VLDL[16(6–68) vs. 13(4–36)mg/dL,p = 0.020] were significantly higher in JDM patients versus controls. Gingival vasculopathy pattern was significantly higher in the former group (60% vs. 0%, p = 0.0001), as well as the median of gingival bleeding index (GBI) [24.1(4.2–69.4) vs. 11.1(0–66.6)%,p = 0.001] and probing pocket depth (PPD) [1.7(0.6–2.4) vs.1.4(0–2.12) mm,p = 0.006]. Comparison between JDM patients with and without dyslipidemia revealed that the median of dental plaque index (PI) [100(26.7–100) vs. 59(25–100)%,p = 0.022], PPD[1.9(0.6–2.4) vs. 1.4(1.2–1.8)mm,p = 0.024] and clinical attachment level (CAL) [1.31(0.7–1.7) vs. 0.8(0.6–1.7)mm,p = 0.005] were significantly higher in patients with dyslipidemia. Further analysis between JDM patients with and without gingivitis revealed that the median of current age [12.4 (8.3–18.4) vs. 9.2 (5.5–17.5) years, p = 0.034] and disease duration [7.09 ± 3.07 vs. 3.95 ± 2.1 years, p = 0.008] were significantly higher in the former group. Conclusion: Our study showed that gingival inflammation seems to be related to dyslipidemia in JDM patients, suggesting underlying mechanisms for both complications.

https://doi.org/10.1186/s42358-018-0024-x 289 downloads

10.

Pilares cerâmicos na implantodontia: revisão de literatura
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Sallenave, R. F.

; Vicari, C. B.

; Borba, M.

Cerâmica

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Set 2016, Volume 62 Nº 363 Páginas 305 - 308

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Os pilares protéticos, usados como intermediários entre a prótese e o implante, são feitos de titânio e outros metais, que possuem estrutura confiável. A cor acinzentada desses metais, acrescidos a recessão gengival e a camadas finas de tecidos adjacentes, acaba exposta, o que compromete a estética. Com isso, foram introduzidos os pilares cerâmicos (zircônia, alumina ou zircônia/alumina) que fornecem boas propriedades ópticas, assim como biocompatibilidade e estética duradoura. O objetivo desse estudo foi avaliar, através de uma revisão sistemática da literatura, as taxas de sobrevivência dos pilares metálicos e cerâmicos, bem como seus principais tipos de falha. Foi realizada uma pesquisa nas bases de dados Pubmed de 1999 a 2015 utilizando as palavras-chave: cerâmicas (ceramics), pilar (abutment), implantes dentários (dental implants) e prótese parcial fixa (partial fixed denture). Foram selecionados 16 artigos de pesquisa clínica e estudos de coorte. Segundo os estudos, não houve diferença significativa nos índices biológicos e radiográficos entre os pilares cerâmicos e metálicos, quando comparados uns com os outros e com os dentes naturais, em estudos de até 11 anos. Também não foi observada perda significativa de osso marginal entre a avaliação inicial e a última avaliação para ambos tipos de pilares (titânio e cerâmicos). Pilares de alumina têm taxa de sobrevivência de 94,7% a 100% em estudo de acompanhamento clínico de até 7 anos. Pilares de zircônia têm taxa de sobrevivência semelhante aos pilares de titânio (100%) em estudos de acompanhamento clínico de até 11 anos. Apenas em um estudo de 7 anos, a taxa de sobrevivência caiu para 97,6%, para os pilares de zircônia. Os modos de falha encontrados para os pilares foram fratura e afrouxamento do parafuso. A semelhança no comportamento mecânico e biológico entre os materiais torna os pilares cerâmicos uma boa alternativa na Implantodontia.

Abstract Prosthetic abutments, used as a connection between the restoration and the implant, are usually produced using titanium and other metals, which provide a reliable structure. However, the grey color from the metal abutment combined with thin gingival tissue may compromise the final treatment aesthetics. Therefore, ceramic abutments were introduced for implant-supported restorations (zirconia, alumina, zirconia/alumina). Ceramic abutments show good optical properties as well as high biocompatibility and durable aesthetics. The objective of this study was to evaluate, through a systematic literature review, the survival rate of titanium and ceramic abutments for implant-supported restorations, as well as the most frequent failure modes. The research was performed using Medline/Pubmed online database with articles published from 1999 to 2015 and the keywords: ceramics, abutment, dental implants, partial fixed denture. Sixteen clinical trial and cohort studies were selected. According to the literature, there were no significant differences for biological and radiographic indexes among metals and ceramic abutments, and among natural teeth, in 11-years prospective studies. No significant bone loss was observed between the first and the last evaluation, for both types of abutments. Alumina-based abutments had 94.7% to 100% survival rate when followed up to 7 years. Zirconia-based abutments showed similar survival rate when compared to titanium abutments (100%) after 11 years. Only in one 7-years study, the survival rate decreased to 97.6%, for zirconia-based abutments. Fracture and screw loosening were the failures modes observed for the abutments. The mechanical and biological similarity among the materials suggests that ceramic abutments are a good treatment option for implant-supported restorations.

https://doi.org/10.1590/0366-69132016623632026 6826 downloads

11.

Bioimpedance as a tool for evaluating the body composition of suruvi (Steindachneridion scriptum)
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Zaniboni-Filho, E.

; Hermes-Silva, S.

; Weingartner, M.

; Jimenez, J. E.

; Borba, M. R.

; Fracalossi, D. M.

Brazilian Journal of Biology

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Nov 2015, Volume 75 Nº 4 Páginas 239 - 244

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A análise da impedância bioelétrica (BIA) é considerada uma importante ferramenta para avaliar a composição corporal de diferentes animais de uma maneira rápida, não-destrutiva e de baixo custo. A espécie Sul-americana Steindachneridion scriptum, popularmente conhecida como suruvi, foi selecionada para este estudo. Foi utilizado um protocolo para produzir peixes com distintas composições corporais, permitindo a validação da BIA para análise adequada da composição corporal do suruvi. Os peixes foram alimentados duas vezes ao dia com duas dietas diferentes: uma de baixo teor lipídico (8,90%) e outra de alto teor lipídico (18,68%). Essa diferença nas dietas possibilitou a produção de indivíduos com diferentes composições corporais. As leituras da BIA foram determinadas utilizando-se o equipamento Quantum X Bioelectrical Body Composition Analyzer. Duas leituras (dorsal e ventral) foram obtidas para cada peixe. Após as leituras, para cada peixe individualmente, a composição proximal dos peixes foi determinada. Todos os dados obtidos foram utilizados para estabelecer as equações de correlação entre as análises proximais e os valores da BIA. Fortes correlações foram encontradas para S. scriptum. As maiores correlações foram obtidas para as análises dorsais a seguir: umidade e resistência em série (R2 = 0,87); proteína e resistência em série (R2 = 0,87); cinzas e reactância em paralelo (R2 = 0,82). Pode-se concluir que o método BIA é eficiente em determinar a composição corporal do suruvi S. scriptum sem sacrificar o animal. No entanto, para expandir o uso desta nova tecnologia é necessário definir protocolos rigorosos para garantir estimativas precisas.

Abstract Bioelectrical impedance analysis (BIA) is regarded as an important tool for evaluating the body composition of different animals in a rapid, non-destructive, and low-cost manner. A South American fish species, Steindachneridion scriptum, known as suruvi, was selected for study in this investigation. A protocol to produce fish with different body composition was used to allow BIA to adequately predict the body composition of suruvi. The fish were fed twice each day with two different diets; a low lipid diet (8.90%), and a high lipid diet (18.68%). These dietary differences allowed suruvi specimens with different body compositions to be produced. The BIA readings were determined using a Quantum X Bioelectrical Body Composition Analyzer. Two readings (dorsal and ventral) were obtained for each fish. After BIA readings were obtained, the proximate composition of the fish bodies for each individual was determined. All of the study data were used to establish correlation equations between proximate analyses and BIA values. Strong correlations were found for S. scriptum. The highest correlations were obtained for the following pairs of quantities, using BIA data from dorsal readings: moisture and resistance in series (R2 = 0.87); protein and resistance in series (R2 = 0.87); and ash and reactance in parallel (R2 = 0.82). We conclude that BIA is an effective method in determining the body composition of S. scriptum without sacrificing the fish. However, to expand the use of this new technology it is important to define strict BIA protocols to guarantee accurate estimates.

https://doi.org/10.1590/1519-6984.13614 1887 downloads

12.

ADSORPTION OF THE DYE REACTIVE BLUE 5G IN RETORTED SHALE
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Lambrecht, R.

; Barros, M. A. S. D. de

; Arroyo, P. A.

; Borba, C. E.

; Silva, E. A. da

Brazilian Journal of Chemical Engineering

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Mar 2015, Volume 32 Nº 1 Páginas 269 - 281

Resumo: En | Texto: En | PDF: En

Abstract In this study the influence of the volumetric flow rate and feed concentration was investigated for the adsorption of the reactive dye Blue 5G. Experiments were carried out in a bed packed with retorted shale, at 30 ºC. The ranges investigated were flow rate 2 -10 mL/min and the feed concentration 13-105 mg/L. Mathematical models were used to represent the dynamic sorption. The double resistance model considers the effects of the axial dispersion and the mass transfer resistance of the external film and inside the particles. As a result, the mass transfer coefficient of the external film and the internal mass transfer coefficient were estimated. The Thomas model was used to simulate the experimental data. In this model the fitted parameter was the adsorption kinetic constant. The first model provided an acceptable representation of the dynamic uptake of the reactive dye Blue 5G.

https://doi.org/10.1590/0104-6632.20150321s00001715 2318 downloads

13.

Heterogeneidade na apresentação clínica de fraturas atípicas após uso prolongado de bisfosfonatos – Fatores de risco e marcadores de remodelação óssea
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Piazzetta, Giovana

; Baracho, Filipe R.

; Oliveira, Larissa de

; Santos, Gustavo R.

; Kulak, Carolina A. M.

; Borba, Victória Z. C.

Arquivos Brasileiros de Endocrinologia & Metabologia

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Nov 2014, Volume 58 Nº 8 Páginas 855 - 861

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Descrevemos quatro casos de fraturas femorais atípicas atendidos no Serviço de Endocrinologia do Hospital de Clínicas da Universidade Federal do Paraná (SEMPR/UFPR) que, apesar de característicos desse tipo de fratura, apresentam peculiaridades clínicas que devem ser consideradas e servir de alerta nesses pacientes, tais como: diagnóstico tardio com manutenção do uso de bisfosfonatos; ausência de comorbidades associadas com resolução sem sequelas; falha na consolidação da fratura; uso de medicação anabólica após a fratura e acompanhamento com marcadores de remodelação óssea.

We describe four cases of atypical femoral fractures treated at the Department of Endocrinology, Hospital de Clínicas, Federal University of Paraná (SEMPR) which, although characteristic of this type of fracture, presented clinical peculiarities that should be considered and serve as a warning in these patients, such as: late diagnosis with maintenance of bisphosphonates; absence of co-morbidities with excellent result; failure of fracture healing; use of anabolic medication after the fracture and the use of bone turnover markers at the follow up.

https://doi.org/10.1590/0004-2730000003229 7952 downloads

14.

Eimeria spp. from Japanese quails (Coturnix japonica): new characteristic features and diagnostic tools
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Berto, Bruno P.

; Borba, Helcio R.

; Lima, Viviane M.

; Flausino, Walter

; Teixeira-Filho, Walter L.

; Lopes, Carlos Wilson G.

Pesquisa Veterinária Brasileira

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Dez 2013, Volume 33 Nº 12 Páginas 1441 - 1447

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

A codorna japonesa Coturnix japonica originária do norte da África, Europa e Ásia, é utilizada mundialmente como um animal experimental e modelo para avicultura. O presente trabalho caracteriza Eimeria bateri, Eimeria tsunodai e Eimeria uzura recuperadas de C. japonica. Baseado no fato de que as codornas têm uma distribuição global, como são os seus coccídios, os resultados deste estudo devem propiciar o diagnóstico destas Eimeria spp. em outras regiões e continentes. Eimeria bateri demonstrou a maior intensidade de infecção e eliminaram oocistos a partir do quarto dia após infecção, em contraste E. tsunodai e E. uzura eliminaram oocistos a partir do quinto dia após infecção. As três espécies foram morfometricamente semelhantes e polimórficas. No entanto, a aplicação da regressão linear, histogramas e ANOVA proveram meios para a identificação destas espécies. Finalmente, o algoritmo foi totalmente eficiente uma vez que valores resultantes não foram sobrepostos.

The Japanese quail Coturnix japonica originated from North Africa, Europe and Asia, is used worldwide as an experimental animal and model for aviculture. The current paper characterizes Eimeria bateri, Eimeria tsunodai and Eimeria uzura recovered from C. japonica. Based on the fact that quails have a global distribution, as are their coccidia, the findings of this study should provide the means for diagnosis of those Eimeria spp. in other regions and continents. Eimeria bateri showed the greatest intensity of infection and shed oocysts from the fourth day after infection; in contrast, E. tsunodai and E. uzura shed oocysts from the fifth day after infection. The three species shared a high degree of similarity and were all polymorphic. Yet, the application of line regressions, histograms and ANOVA provided means for the identification of these species. Finally, the algorithm was very efficient since verified that resultant values were not superimposed.

4271 downloads

15.

National prevalence survey in Brazil to evaluate the quality of microbiology laboratories: the importance of defining priorities to allocate limited resources
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Costa, Luciano B.

; Cardoso, Maria Regina Alves

; Ferreira, Consuelo G.

; Levy, Carlos E.

; Borba, Heder M.

; Sallas, Janaina

; Santana, Heiko T.

; Lima, Rogério S.

; Ferraz, Lucia R.

; Reis, Joana D'Arc P.

; Santi, Leandro Q.

; Levin, Anna S.

Revista Panamericana de Salud Pública

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Jan 2013, Volume 33 Nº 1 Páginas 73 - 78

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

This report describes a survey of microbiology laboratories (n = 467) serving Brazilian hospitals with >10 intensive care beds and/or involved in the government health care adverse event reporting system. Coordinators were interviewed and laboratories classified as follows: Level 0 (no minimal functioning conditions-85.4% of laboratories); Level 1 (minimal functioning conditions but inadequate execution of basic routine-6.7%); Level 2 (minimal functioning conditions and adequate execution of basic routine but no adequate procedures for quality control-5.8%); Level 3 (minimal functioning conditions, adequate execution of basic routine, and adequate procedures for quality control, but no direct communication with the infection control department-0.9%); Level 4 (minimal functioning conditions, adequate execution of basic routine, adequate procedures for quality control, and direct communication with infection control, but no available advanced resources-none); and Level 5 (minimal functioning conditions, adequate execution of basic routine, adequate procedures for quality control, direct communication with infection control, and available advanced resources-0.9%). Twelve laboratories did not perform Ziehl-Neelsen staining; 271 did not have safety cabinets; and >30% without safety cabinets had automated systems. Low quality was associated with serving hospitals not participating in government adverse-event program; private hospitals; nonteaching hospitals; and those outside state capitals. Results may reflect what occurs in many other countries where defining priorities is important due to limited resources.

Este artículo describe una encuesta realizada en Brasil en laboratorios de microbiología (n = 467) que prestaban servicio a hospitales que contaban al menos con 10 camas de cuidados intensivos. Se entrevistó a los coordinadores y los laboratorios se clasificaron de la siguiente manera: nivel 0 (sin condiciones de funcionamiento mínimas: 85,4% de los laboratorios), nivel 1 (condiciones de funcionamiento mínimas pero ejecución inadecuada del trabajo habitual básico: 6,7%), nivel 2 (condiciones de funcionamiento mínimas y ejecución adecuada del trabajo habitual básico, pero sin procedimientos de control de calidad apropiados: 5,8%), nivel 3 (condiciones de funcionamiento mínimas, ejecución adecuada del trabajo habitual básico y procedimientos de control de calidad apropiados, pero sin comunicación directa con el departamento de control de infecciones: 0,9%), nivel 4 (condiciones de funcionamiento mínimas, ejecución adecuada del trabajo habitual básico, procedimientos de control de calidad apropiados y comunicación directa con el departamento de control de infecciones, pero sin recursos avanzados disponibles: ningún laboratorio) y nivel 5 (condiciones de funcionamiento mínimas, ejecución adecuada del trabajo habitual básico, procedimientos de control de calidad apropiados, comunicación directa con el departamento de control de infecciones y recursos avanzados disponibles: 0,9%). Doce laboratorios no realizaban la tinción de Ziehl-Neelsen, 271 no contaban con cámaras de seguridad biológica, y más de 30% de los laboratorios que carecían de cámaras de seguridad biológica tenían sistemas automatizados. La escasa calidad se asoció a la falta de participación en el programa gubernamental de notificación de acontecimientos adversos, a los hospitales privados, a los hospitales no docentes y a la ubicación de los hospitales fuera de las capitales de los estados. Los resultados pueden reflejar lo que ocurre en muchos otros países con recursos limitados, donde es importante definir las prioridades.

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Você pode enriquecer sua busca de uma forma muito simples. Use os índices de pesquisa combinados com os conectores (AND ou OR) e especifique cada vez mais sua busca.

Por exemplo, se você deseja buscar artigos sobre
casos de dengue no Brasil em 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

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