RESUMO O objetivo deste estudo foi avaliar a produtividade, as características morfométricas e o valor nutricional do milho, com uso de fungicida foliar associado a inoculante na ensilagem, sobre as características químico-fermentativas da silagem. As aplicações do fungicida piraclostrobina + fluxpiroxade foram realizadas nos estádios vegetativo-oito e pendoamento, e as avaliações das plantas ocorreram simultaneamente na colheita (fase de grãos pastosos), quando foi aplicado o inoculante composto por cepas de Lactobacillus buchneri e L. casei para a produção de silagem. O uso de fungicida diminuiu a porcentagem de área foliar afetada por Diplodia macrospora Earle, Cercospora zeae-maydis e Phaeosphaeria maydis. Na colheita, o milho apresentou menor número de folhas secas e maior produtividade de biomassa fresca (66.368 vs. 62.015kg·ha-1) e seca (20.964 vs. 19.485kg·ha-1) com fungicida. O fungicida reduziu teor de LDA de 5,99% para 5,16%, gerando maior ISDMD para planta inteira (43,14 e 62,57%, para 24 e 48 horas, respectivamente). A associação do fungicida com inoculante promoveu maior concentração de ácido acético do que quando cada um foi utilizado isoladamente, e as perdas de matéria seca da silagem com inoculante foram maiores que as da silagem controle (8,88 vs. 12,78%, respectivamente). Fungicida e inoculante utilizados associados proporcionaram silagens com menor teor fibroso. ensilagem químicofermentativas químico fermentativas vegetativooito vegetativo oito pendoamento fase pastosos, pastosos , pastosos) L Earle zeaemaydis zeae maydis 66.368 66368 66 368 (66.36 vs 62.015kg·ha1 62015kgha1 kgha 62.015kg·ha 1 62 015kg ha kg 62.015kg·ha-1 20.964 20964 20 964 (20.96 19.485kg·ha1 19485kgha1 19.485kg·ha 19 485kg 19.485kg·ha-1 599 5 99 5,99 516 16 5,16% 43,14 4314 43 14 (43,1 6257 57 62,57% 2 4 horas respectivamente. respectivamente . respectivamente) isoladamente 8,88 888 8 88 (8,8 1278 12 78 12,78% fibroso 66.36 6636 6 36 (66.3 ha1 62015kgha 62.015kg·ha- 20.96 2096 96 (20.9 19485kgha 19.485kg·ha- 59 9 5,9 51 5,16 43,1 431 (43, 625 62,57 8,8 (8, 127 7 12,78 66.3 663 3 (66. 20.9 209 (20. 5, 5,1 43, (43 62,5 8, (8 12,7 66. (66 20. (20 (4 62, ( 12, (6 (2

ABSTRACT The aim of this study was to evaluate the yield, the morphometric characteristics, and the nutritional value of corn with use of foliar fungicide associated with inoculant on ensiling on the chemical-fermentative characteristics of silage. The applications of fungicide pyraclostrobin + fluxpyroxade were carried out in vegetative-eight and tasseling stage, and the evaluations of plants occurred simultaneously at harvest (dough grain stage), during which application was made with inoculant consisting of strains of Lactobacillus buchneri and L. casei for silage production. The use of fungicide decreased the percentage of leaf area affected by Diplodia macrospora Earle, Cercospora zeae-maydis and Phaeosphaeria maydis. At harvest, corn showed fewer dry leaves and higher yields of fresh (66,368vs. 62,015kg·ha-1) and dry biomass (20,964 vs. 19,485kg·ha-1) with fungicide. The fungicide also reduced the LDA content from 5.99% to 5.16%, which generated greater ISDMD for whole plant (43.14 and 62.57%, for 24 and 48 hours, respectively). The association of fungicide with inoculant promoted higher concentration of acetic acid than when each was used alone, and the dry matter losses of silage with inoculant were higher than control silage (8.88vs. 12.78%, respectively). Fungicide and inoculant used in combination provided silages with lower fibrous content. yield chemicalfermentative chemical fermentative vegetativeeight vegetative eight stage dough , stage) L production Earle zeaemaydis zeae maydis 66,368vs. 66368vs vs 66 368vs (66,368vs 62,015kg·ha1 62015kgha1 kgha 62,015kg·ha 1 62 015kg ha kg 62,015kg·ha-1 20,964 20964 20 964 (20,96 19,485kg·ha1 19485kgha1 19,485kg·ha 19 485kg 19,485kg·ha-1 599 5 99 5.99 516 16 5.16% 43.14 4314 43 14 (43.1 6257 57 62.57% 2 4 hours respectively. respectively . respectively) alone 8.88vs. 888vs 8 88vs (8.88vs 1278 12 78 12.78% 66,368vs 6 ha1 62015kgha 62,015kg·ha- 20,96 2096 96 (20,9 19485kgha 19,485kg·ha- 59 9 5.9 51 5.16 43.1 431 (43. 625 62.57 8.88vs 127 7 12.78 20,9 209 (20, 5. 5.1 43. (43 62.5 12.7 20, (20 (4 62. 12. (2 (

Abstract This study aimed to determine whether the addition of green propolis extract to the diet of laying hens would improve egg quality and bird performance and decrease bacterial contamination of eggs. Forty-five brown Hy-line laying hens were used, divided into five groups with three replicates each and three animals per cage: T0 - diet without propolis; T5 - 5 grams of propolis per kg of feed; T10 - 10 grams of propolis per kg of feed; T20 - 20 grams of propolis per kg of feed and T30 - 30 grams of propolis per kg of feed. The quality of fresh eggs was made on day 21 of the experiment, and eggs were stored for 21 days. Greater specific gravity was observed in fresh eggs in T5 birds and stored eggs for T10. TBARS in fresh eggs, we found that T30 eggs had lower levels compared to other treatments. T20 eggs had the lowest total bacterial count and the lowest total coliform count in the eggshells of T10 and T20 chickens. The count of E. coli in the peel was lower for T20 and T30 than T0 and T5. The consumption of propolis did not interfere with the hens’ egg production rate but reduced food intake and consequently reduced feed conversion. We concluded that the addition of green propolis in the diet of laying birds proved efficient in reducing bacterial contamination in the eggshells and reducing the lipid peroxidation of fresh and stored eggs.

ABSTRACT The aim of this study was to evaluate the efficacy and safety of several anesthetics in the brachyuran crab Neohelice granulata, an emergent experimental model. The essential oils (EOs) of Lippia alba, Aloysia tryphilla, and Melaleuca alternifolia (tea tree oil; TTO), the isolated compounds eugenol, menthol, terpinen-4-ol, and the nanoencapsulated form of TTO, were administered in one or more of the following ways: added to the water (immersion), through an arthrodial membrane (injected), or by oral gavage. Unexpectedly, most EOs did not produce an anesthetic effect after immersion. Only TTO and eugenol induced anesthesia by immersion, with very long induction and recovery times compared to anesthesia of other crustaceans. However, a good anesthetic effect was observed with the injection of terpinen-4-ol and nanoencapsulated TTO in N. granulata; both demonstrated ideal induction and recovery times. These substances appear to be promising anesthetic alternatives for crustaceans.

Abstract This study was aimed to analyze the effects of spray-dried porcine plasma (SDPP) on the health of post weaning piglets challenged with diets contaminated with aflatoxins and fumonisins. Fifty-six male piglets (7.15 ± 0.61 kg) were allocated in four groups: CTL group received a regular diet; SDPP group received a regular diet and 6% SDPP; MYC group received a diet containing 300 µg/kg aflatoxins and 8,000 µg/kg fumonisins; group MYC+SDPP received 300 µg/kg aflatoxins, 8,000 µg/kg fumonisins and 6% SDPP. The animals that received the experimental diet containing mycotoxins (MYC group) had lower weight gain at the end of the experiment compared to the other treatments. Animals receiving SDPP showed decreased urea levels throughout the experiment (P<0.05). Animals from MYC group presented an increased on reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) levels and decreased catalase activity (P<0.05). In contrast, SDPP prevented the increase of ROS and TBARS and stimulated superoxide dismutase activity (P<0.05). In conclusion, diet contaminated with mycotoxins (group MYC) caused subclinical intoxication in the piglets, as observed by the increase on free radical’s production and lipid peroxidation. Conversely, SDPP presented a protective effect, minimizing the effects of oxidative stress caused by aflatoxins and fumonisins ingestion.

ABSTRACT The aim of this study was to modify the fatty acid profile of yogurt from sheep milk by the inclusion of different concentrations of palm oil into their diet. Thus, thirty-six sheep during lactation were separated in four groups with nine animals each, as described below: the group T0 (0%); the group T2 (inclusion of 2% of palm oil); the group T4 (inclusion of 4% of palm oil) and the group T6 (inclusion of 6% of palm oil). After 60 days of the supplementation, milk samples were collected and yogurt was produced, which was evaluated regarding the concentration of saturated fatty acids (SFA), monounsaturated fatty acids (MFA), and polyunsaturated fatty acids (PFA). A significant reduction (p<0.05) in most SFA and a significant increase (p<0.05) on MFA and PFA was observed in the yogurt of sheep supplemented with 4 and 6% of palm oil. Consequently, it is possible to conclude that palm oil supplementation exerts positive effects on yogurt, since it led to the reduction of undesirable fatty acids and increased fatty acids beneficial to human health.

No final da década de 70, ocorreu o primeiro relato de sucesso com a utilização de quimioterapia de alta dose, seguida de transplante de células-tronco hematopoéticas autólogo (TCTH auto), em pacientes com linfoma não Hodgkin (LNH). Desde então, o TCTH autólogo vem se constituindo em um importante instrumento na estratégia de tratamento dos LNH. Inúmeros estudos, em vários subtipos de linfomas, têm consolidado o papel do TCTH autólogo, principalmente como resgate em recidivas de doença. O melhor momento para a incorporação desta estratégia depende do subtipo do linfoma, do status de doença previamente ao transplante (sensível ou resistente) e de fatores clínico-biológicos associados à doença. Em recidiva sensível de linfoma difuso de grandes células, o TCTH autólogo é a terapia de escolha. Nestes pacientes, o transplante promove taxas de resposta completa em até 50% dos casos, comparado a aproximadamente 15%, quando esse resgate é realizado com protocolos quimioterápicos convencionais. O seu papel como parte da terapia de indução de remissão não está totalmente estabelecido. Em linfomas indolentes, principalmente folicular, é a terapia de escolha nas recidivas sensíveis à quimioterapia de resgate. Em linfomas de células do manto, o TCTH autólogo tem se incorporado à terapia de primeira linha, como consolidação de remissão. As indicações de TCTH alogênico em LNH têm se limitado aos casos de refratariedade ao tratamento convencional e recidiva pós-transplante autólogo, em pacientes jovens e sem comorbidades, em decorrência da alta toxicidade associada à utilização de regimes de condicionamento mieloablativos. A utilização de regimes de condicionamento de intensidade reduzida tem reduzido a toxicidade e ampliado o seu uso nos LNH recidivados ou refratários.

High-dose chemotherapy (HDT) followed by autologous bone marrow transplantation (ABMT) has proved to provide significant advantage regarding event-free and overall survival in patients with chemosensitive relapses of aggressive non-Hodgkin's lymphoma (NHL) after conventional therapy. These results encouraged many investigators to use HDT as part of first-line therapy but the results are contradictory. There is no consensus regarding management of relapsed or refractory DLBCL. In follicular lymphomas, autologous stem cell transplantation (SCT) is considered the treatment of choice for young patients with relapsed disease. Autologous SCT has also been evaluated in prospective trials as first-line treatment for high risk patients at diagnosis, but the results are not yet conclusive. In mantle cell lymphoma, autologous stem cell transplantation has been employed as part of first-line therapy. Allo-SCT for patients with lymphoma was first performed in the mid-1980s. The high transplant-related mortality, seen after myeloablative conditioning, discouraged broader interest in this approach and made further research difficult. The generally lower relapse rates after allo-SCT, the association of GvHD with reduced relapse rates, the increase of relapse rates after ex vivo or in vivo T-cell depletion, and the frequent responses to DLIs all support the existence of a graft-vs.-lymphoma effect. However, further data analysis supports the view that not all lymphomas are equal. While slowly proliferating diseases such as follicular lymphoma seem particularly sensitive targets for allogeneic T-cells, results of allo-SCT with aggressive B-cell lymphomas have been less convincing. Patients with these latter diseases obviously need vigorous debulking of their tumor prior to conditioning. Reduced-intensity conditioning fueled a renaissance of allo-SCT as treatment of lymphoma because the lower expected TRM was highly attractive for a patient population where the transplant-related death rate after myeloablative conditioning had, in many instances, exceeded 50%.

An aliphatic-aromatic copolyester of poly(ethylene terephthalate), PET, and poly(ethylene adipate), PEA, PET-co-PEA, was synthesized by the high temperature melt reaction of post-consumer PET and PEA. As observed by NMR spectroscopy, the reaction yielded random copolyesters in a few minutes through ester-interchange reactions, even without added catalyst. The copolyesters obtained in the presence of a catalyst presented higher intrinsic viscosity than that obtained without the addition of catalyst, due to simultaneous polycondensation and ester-interchange reactions. The structure of the aliphatic-aromatic copolyesters obtained in different PET/PEA ratio is random as observed by NMR analysis.