Abstract Biorefineries require low-cost production processes, low waste generation and equipment that can be used not only for a single process, but for the manufacture of several products. In this context, in this research a continuous 3D printing microbioreactor coupled to an Arduino-controlled automatic feeding system was developed for the intensification of the ethanol production process from xylose/xylulose (3:1), using a new biocatalyst containing the co-culture of Scheffersomyces stipitis and Saccharomyces cerevisiae (50/50). Initially, batch fermentations of monocultures of S. cerevisiae and S. stipitis and co-culture were carried out. Subsequently, the immobilized co-culture was used as a biocatalyst in continuous fermentations using the developed microreactor. Fermentations carried out in the microbioreactor presented a 2-fold increase in the ethanol concentration and a 3-fold increase in productivity when compared to monocultures. The microbioreactor developed proved to be efficient and can be extended for other bioproducts production. This approach proved to be a promising alternative for the use of the hemicellulose fraction of biomasses without the need to use modified strains. lowcost cost processes products context D Arduinocontrolled Arduino controlled xylosexylulose xylose xylulose 31, 31 3 1 , (3:1) coculture co culture 50/50. 5050 50/50 . 50 (50/50) Initially S Subsequently microreactor 2fold fold 2 3fold strains (3:1 505 50/5 5 (50/50 (3: 50/ (50/5 (3 (50/ ( (50 (5

This work describes the synthesis and application of an europium(III) complex, [Eu(TTA)3(2-pyr) (H2O)], containing 2-thenoyltrifuoroacetonate (TTA) and 2-pyrrolidone (2-pyr) as ligands, obtained as white solid and soluble in ethanol. In solution, the complex showed red emission, characteristic of the EuIII emission in the solid state (λexc = 375 nm and λem = 612 nm). The complex was tested with PbII, CdII, and HgII ions in water. The effect of metal ions was monitored and evaluated by hypersensitive transition 5D0 → 7F2 (612 nm). The results show that PbII suppresses the complex luminescence and present high sensitivity, according to the values for the Stern-Volmer constant (K SV = 2300 L mol−1), showing good linearity, i.e., determination coefficient (R 2) of 0.9913, and low limit of detection (LOD = 6.03 µM). Luminescence quenching increased with the increasing concentration of PbII. These results indicate that the synthesized complex is a potential luminescent sensor for detecting PbII in a simple and fast way, being applicable for routine environmental analysis. When applied as an ethanol sensor in gasoline, the complex hypersensitive transition intensity decreased with the increasing volume of ethanol in gasoline, reaching the values of R 2 = 0.9815 and LOD = 4.94% v/v.

A eosinofilia no sangue e em tecidos está habitualmente associada a condições alérgicas, infecciosas, inflamatórias, neoplásicas, endocrinológicas, uso de medicamentos e exposição a agentes tóxicos. No entanto, pode ocorrer proliferação eosinofílica primária, sem causa aparente ou por expansão clonal da célula-tronco hematopoética. As neoplasias mielo ou linfoproliferativas associadas a rearranjos gênicos como PDGFRα, PDGFRβ e FGFR1 constituem condições raras nas quais ocorre mieloproliferação crônica, alterações no sangue periférico e na medula óssea e lesão tecidual de diferentes órgãos, a partir da liberação de citocinas e fatores humorais pelos grânulos eosinofílicos. A presença do rearranjo PDGFRα relaciona-se comumente à leucemia eosinofílica crônica, com envolvimento de mastócitos e neutrófilos e, mais raramente, à leucemia mielóide aguda ou ao linfoma linfoblástico T, com eosinofilia. O rearranjo PDGFRα mais comum é aquele resultante da deleção intersticial no braço longo do cromossomo 4, que permite a formação de um neogene a partir da fusão dos genes FIP1L1 e PDGFRα. Este codifica uma tirosino-quinase constitutivamente ativa que é inibida pelo mesilato de imatinibe. Em 2002 foi relatado o uso bem sucedido de mesilato de imatinibe em baixas doses em um paciente com síndrome hipereosinofilica e, desde então, vem-se utilizando esta droga com respostas clínicas rápidas, completas e duradouras. Descrevemos um caso de LEC com expressão do rearranjo FIP1L1-PDGFRα.

Chronic eosinophilia is habitually associated with allergic, infectious, inflammatory, neoplastic and endocrine conditions and exposure to certain drugs and toxic agents. However, eosinophilic proliferation may be primary, without identifiable causes, or provoked by clonal hematopoietic stem cell proliferation. Gene fusions involving PDGFR-α, PDGFR-β, and FGFR1 predispose patients to rare conditions with chronic myeloproliferation or lymphoproliferation, alterations in peripheral blood and bone marrow and diffuse tissue injury due to the release of cytokines and humoral factors from eosinophilic granules. The presence of the PDGFR-α rearrangement is commonly related to chronic eosinophilic leukemia, with alterations in peripheral mastocytes and neutrophils, and rarely to acute myeloid leukemia or T lymphoblastic lymphoma with eosinophilia. The most prevalent PDGFR-α rearrangement is one resulting from an interstitial deletion in the long arm of chromosome 4, that allows the formation of a neogene from the fusion of the FIP1L1 and PDGFRα genes. This codes a constitutively active tyrosine kinase, which can be inhibited by imatinib mesylate. In 2002, the successful treatment of a patient using imatinib to treat hypereosinophilic syndrome was reported. Since then, this drug has been utilized with fast, complete and lasting clinical responses. Here we describe a case of chronic eosinophilic syndrome with expression of the FIP1L1-PDGFR-α rearrangement.

OBJETIVO: O objetivo desse estudo in vitro foi de avaliar a ação antimicrobiana de enxaguatórios bucais com e sem álcool. METODOLOGIA: Usaram-se no estudo o Periogard®, o CEPACOL®, Plax Classic® e o ORAL-B®, anti-séptico sem álcool. Para a realização desse estudo foram utilizadas 40 placas de Petri com meio de cultura de Ágar Sangue para os seguintes microrganismos: Staphylococcus aureus, Candida albicans, Enterococcus faecalis, Pseudomonas aeruginosa. Para a mensuração dos halos de inibição, um único examinador treinado utilizou uma lupa estereoscópica e um paquímetro digital. Após a coleta dos dados, as médias foram comparadas utilizando-se do teste estatístico ANOVA (análise de variância) com teste corretivo de Bonferroni, para nível de significância de 5%. RESULTADOS: O Periogard® com álcool e o sem álcool apresentaram os melhores resultados. O Plax® sem álcool obteve os piores resultados. O Plax® com álcool foi melhor substância em relação ao S. aureus. Os demais resultados mantiveram-se com melhor efetividade em relação à substância controle. CONCLUSÃO: Os enxaguatórios sem álcool não têm a mesma eficácia antimicrobiana comparada aos enxaguatórios com álcool em relação aos microrganismos testados neste estudo.

PURPOSE: The aim of this in vitro study was to evaluate the effect of mouthwashes with and without alcohol on some microorganisms. METHODS: Periogard®, Cepacol®, Plax Classic® and Oral-B® were the substances tested. For this study, 40 plates of Petri with medium blood agar were used for the following microorganisms: Staphylococcus aureus, Candida albicans, Enterococcus faecalis, Pseudomonas aeruginosa. To measure the inhibition zones, a single trained examiner used a stereomicroscope and a digital caliper. After data collection, the medias were compared using the ANOVA (analysis of variance) with Bonferroni test post-hoc correction for 5% significance level. RESULTS: Periogard®, with and without alcohol, showed the best results. Plax® without alcohol presented the poorest results. Plax® with alcohol was the best substance in relation to S. aureus. The others results remained with better effectiveness in relation to control substance. CONCLUSION: The alcohol-free mouthwashes did not have the same efficacy of antimicrobial rinses with alcohol in relation to microorganisms tested in this study.

OBJETIVO: Analisar a ação antimicrobiana da clorexidina a 2%, do hipoclorito de sódio a 1% e do paramonoclorofenol associado ao furacin sobre S aureus, C albicans, E faecalise e P aureginosa. METODOLOGIA: Foram utilizadas 40 placas Petri. Quatro placas foram separadas como controle negativo e em 4 outras, além do meio de cultura, semearam-se os microrganismos, com o círculo de papel, para se obter o controle positivo. Em 32 placas seguiu-se a colocação de 4 círculos de filtro de papel esterilizados e impregnados das substâncias testes e controle, depositados em cada quadrante das mesmas. Os grupos foram analisados por 7 dias. Para a verificação dos resultados, usaram-se os halos de inibição de crescimento bacteriano. RESULTADOS: A clorexidina 2% foi significantemente (P<0,05) mais efetiva para todas as cepas microbianas que as demais substâncias. O hipoclorito de sódio a 1% apresentou resultados intermediários. O paramonoclorofenol associado ao furacin (PMC+F) obteve os piores resultados. CONCLUSÃO: A clorexidina obteve os melhores resultados. O PMC+F apresentou os menores halos de inibição.

PURPOSE: To analyze the antimicrobial action of 2% chlorhexidine gluconate, 1% sodium hypochlorite and paramonochlorophenol were combined with furacin against strains of S. aureus, C. albicans, E. faecalis and P. aureginosa. METHODS: Forty Petri plates were used. Four plates were separated from the others and used as a negative control. Four other plates were used a positive control. The other 32 plates were treated with four circles of sterilized filter paper impregnated with the test and control substances. The groups of plates were analyzed after an experiment time of seven days by the measuring the inhibition halos. RESULTS: Two percent chlorhexidine gave the largest inhibition zones, and the difference in size between its inhibition zones and the compound with the next largest inhibition zone, 1% sodium hypochlorite, was statistically significant. The 1% sodium hypochlorite also had significantly larger inhibition zones than the control group and PMC+F. CONCLUSION: Two percent chlorhexidine gluconate gave the best results, while PMC+F showed the weakest antibacterial activity.