Pesquisa | SciELO

ABSTRACT. Conservation practices such as no-tillage are necessary to maintain, and/or improve agricultural soil quality. However, straw formation and maintenance in tropical regions is a limiting factor to adopting this system. In this sense, this research aimed to study the effect of cover crops on straw formation, soil quality indicators, and cowpea yield under no-tillage in the Amazon savanna. Two experiments were carried out in consecutive years, on a Latossolo Amarelo. The treatments consisted of the straw of cover crops in a single (S) and intercrop (I) system, as follows: Urochloa brizantha (UBS), Pennisetum glaucum (PGS), Crotalaria juncea (CJS), Canavalia ensiformis (CES), Mucuna aterrima (MAS), U. brizantha and C. juncea (UBCJI), U. brizantha and C. ensiformis (UBCEI), P. glaucum and C. juncea (PGCJI), P. glaucum and C. ensiformis (PGCEI), and spontaneous vegetation (SV). Cowpea was cultivated after desiccation of the cover crops. Most of the straw favored soil particle aggregation, except for C. juncea and SV, which negatively influenced this variable. Basal soil respiration, organic carbon content, potassium, base saturation, geometric mean diameter, macroaggregates, and macroporosity at layer of 0-0.10 m were the most sensitive indicators for improving soil quality in a savanna environment. UBS, UBCJI, and UBCEI were superior to the other treatments in improving soil quality. Almost all treatments increased cowpea yield by up to 29%, except for CJS and SV. ABSTRACT notillage no tillage maintain andor or However system sense years Amarelo S (S I (I follows UBS , (UBS) PGS, PGS (PGS) CJS, (CJS) CES, CES (CES) MAS, MAS (MAS) U C UBCJI (UBCJI) UBCEI, (UBCEI) P PGCJI, PGCJI (PGCJI) PGCEI, PGCEI (PGCEI) SV . (SV) aggregation variable respiration content potassium saturation diameter macroaggregates 00.10 0010 0 0.10 10 0-0.1 environment 29 29% (UBS (PGS (CJS (CES (MAS (UBCJI (UBCEI (PGCJI (PGCEI (SV 00 00.1 001 010 0.1 1 0-0. 2 00. 01 0. 0-0 0-

https://doi.org/10.4025/actasciagron.v46i1.62853

Development of an antibody against EtpA from enterotoxigenic Escherichia coli and evaluation of its use for bacterial isolation using magnetic beads
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ASTOLPHO, HELBER A.

; MARIÚBA, LUIS ANDRÉ M.

; CHAVES, YURY O.

; GLÓRIA, JULIANE C.

; ALMEIDA, MARIA EDILENE M. DE

; MACHADO-DE-ÁVILA, RICARDO A.

; GENEROSO, CAMILLE M.

; BATISTA, JENIFFER C.L.

; NOGUEIRA, PAULO AFONSO

; ORLANDI, PATRÍCIA P.

Anais da Academia Brasileira de Ciências

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2024, Volume 96 Nº 2 elocation e20231208

Resumo: En | Texto: En | PDF: En

Abstract The enterotoxigenic Escherichia coli (ETEC) strain is one of the most frequent causative agents of childhood diarrhea and travelers’ diarrhea in low-and middle-income countries. Among the virulence factors secreted by ETEC, the exoprotein EtpA has been described as an important. In the present study, a new detection tool for enterotoxigenic E. coli bacteria using the EtpA protein was developed. Initially, antigenic sequences of the EtpA protein were selected via in silico prediction. A chimeric recombinant protein, corresponding to the selected regions, was expressed in an E. coli host, purified and used for the immunization of mice. The specific recognition of anti-EtpA IgG antibodies generated was evaluated using flow cytometry. The tests demonstrated that the antibodiesdeveloped were able to recognize the native EtpA protein. By coupling these antibodies to magnetic beads for the capture and detection of ETEC isolates, cytometric analyses showed an increase in sensitivity, specificity and the effectiveness of the method of separation and detection of these pathogens. This is the first report of the use of this methodology for ETEC separation. Future trials may indicate their potential use for isolating these and other pathogens in clinical samples, thus accelerating the diagnosis and treatment of diseases. (ETEC travelers lowand low middleincome middle income countries important study E developed Initially prediction regions host mice antiEtpA anti cytometry isolates sensitivity samples diseases

https://doi.org/10.1590/0001-3765202420231208

Use and recovery of P reserves in Southern Brazil Oxisols under no-till with low and high P availability notill no till
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Amorim, Magno Batista

; Fontoura, Sandra Mara Vieira

; Tiecher, Tales

; Moraes, Renato Paulo de

; Bayer, Cimelio

Revista Brasileira de Ciência do Solo

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2024, Volume 48 Nº spe1 elocation e0230127

Resumo: En | Texto: En | PDF: En

ABSTRACT The accessibility of soil P reserves by plants on highly weathered Brazilian soils remains unclear. In this study, we assessed the contribution of soil P fractions to P uptake by soybean plants in two no-till Oxisols (Latossolos), with low and high initial content of available P. The recommended P rates (viz., 153 and 50 kg ha-1 yr-1 in the low-P and high-P soil, respectively) were applied in the form of triple superphosphate (TSP) or Bayovar rock phosphate (BRP) plus a treatment without fertilizer. Yields of winter cereals and soybean on the studied soils were examined over a period of three years. Soil samples were collected in the 0.00-0.10 m soil layer and subjected to sequential chemical fractionation of P. In the last crop season, the P content of soybean shoot biomass was determined. Soil P fractions and P content of soybean shoots were subjected to path analysis. The addition BRP in the low-P soil resulted in a winter cereal yield of 2.0 Mg ha-1 lower than TSP. In the scenario of high P, it is possible to maintain high crop yields even without phosphate fertilization for three consecutive years. Path analyses demonstrated the addition of P using either TSP or BRP boosted the contribution of the more labile inorganic P fractions. The addition of BRP should be avoided in P-deficient soils. The addition TSP or BRP reduces the pressure of plants to use soil P reserves. Therefore, the soil P legacy should be considered in fertilization recommendations for high-P soils in NT. unclear study notill no till Latossolos, Latossolos , (Latossolos) viz., viz (viz. 15 5 ha1 ha 1 ha- yr1 yr yr- lowP highP respectively (TSP (BRP fertilizer years 0.000.10 000010 0.00 0.10 0 00 10 0.00-0.1 season determined analysis 20 2 2. Pdeficient deficient Therefore NT (Latossolos viz. (viz 000 0.000.1 00001 0.0 010 0.1 0.00-0. 0.000. 0000 0. 01 0.00-0 0.000 0.00-

https://doi.org/10.36783/18069657rbcs20230127

Historical line of airglow observations at Comandante Ferraz Brazilian Station: measurements of temperature and studies on gravity waves Station
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BAGESTON, JOSÉ VALENTIN

; WRASSE, CRISTIANO MAX

; GIONGO, GABRIEL AUGUSTO

; CORREIA, EMÍLIA

; FIGUEIREDO, COSME ALEXANDRE O.B.

; GOBBI, DELANO

; TAKAHASHI, HISAO

; BATISTA, PAULO P.

; COSTA, RICARDO A.B. DA

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 3 elocation e20210836

Resumo: En | Texto: En | PDF: En

Abstract The thermal dynamics of the upper atmosphere, especially in the mesosphere, have been improving our understanding about the effects of climate change, as well as how the dynamics and general circulation in the upper atmosphere are driven by different types of waves, such as the atmospheric gravity waves, planetary waves and atmospheric tides. In order to study the upper atmospheric temperature variability and gravity waves, several research groups have been employing the remote technique of observing the airglow emissions originated in the upper mesosphere and lower thermosphere, besides emissions from the ionosphere. INPE’s airglow group started the studies on emissions from the upper atmosphere to investigate temperature and dynamics in the mesosphere, thermosphere/ionosphere at the end of 70’s. However, only in 2001 this group sent the first airglow experiment to the Brazilian Antarctic Station Comandante Ferraz (EACF) to measure the OH (8-3) emission and temperature. From that year to 2014, several other experiments were carried out at EACF, not only to measure the temperature and airglow intensities, but also to observe gravity waves, winds and other related phenomena. This paper presents airglow experiments at EACF from 2001 to the present, including illustrations, examples of already published results, and unpublished data. change tides thermosphere ionosphere INPEs INPE s thermosphereionosphere 70s. 70s 70 s. 70’s However 200 (EACF 83 8 3 (8-3 2014 intensities phenomena present illustrations results data 7 20 (8- 201 2 (8 (

https://doi.org/10.1590/0001-3765202320210836

Malaria at international borders: challenges for elimination on the remote Brazil-Peru border
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Palma-Cuero, Monica

; Machado, Myrna Barata

; Graça, Jucelia Taiz Bruno

; Anjos, Ney Batista dos

; Pereira, Rafael Santos

; Suárez-Mutis, Martha Cecilia

Revista do Instituto de Medicina Tropical de São Paulo

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2022, Volume 64 elocation e29

Resumo: En | Texto: En | PDF: En

ABSTRACT Understanding local epidemiology is essential to reduce the burden of malaria in complex contexts, such as Brazilian municipalities that share borders with endemic countries. A descriptive study of malaria in the period 2003 to 2020 was conducted using data from the Malaria Epidemiological Surveillance Information System related to a remote municipality with an extensive border with Peru to understand the disease transmission, focusing on the obstacles to its elimination. The transmission increases at the end of the rainy season. During the period of 18 years, 53,575 malaria cases were reported (Mean of API 224.7 cases/1,000), of which 11% were imported from Peru. Thirteen outbreaks of malaria were observed during the studied period, the last one in 2018. The highest burden of cases was caused by P. vivax (73.2%), but P. falciparum was also prevalent at the beginning of the study period (50% in 2006). Several changes in the epidemiological risk were observed: (1) the proportion of international imported cases of malaria changed from 30.7% in 2003 to 3.5% in 2020 (p<0.05); (2) indigenous people affected increased from 24.3% in 2003 to 89.5% in 2020 (p<0.0001); (3) infected children and adolescents < 15 years old increased from 50.2% in 2003 to 67.4% in 2020 (p<0.01); (4) the proportion of men decreased from 56.7% in 2003 to 50.4% in 2020 (p<0.01); (5) the likelihood of P. falciparum malaria has significantly declined (p<0.01). The number of cases and the incidence of malaria in 2019 and 2020 were the lowest in the period of 18 years. The burden of malaria in indigenous areas and its determinants, seasonality, geographical access and the long international border are obstacles for the elimination of malaria that must be overcome.

https://doi.org/10.1590/s1678-9946202264029

Cover crop increases soybean yield cropped after degraded pasture in sandy soil
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Cordeiro, Carlos F. dos S.

; Batista, Guilherme D.

; Lopes, Bruno P.

; Echer, Fábio R.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Ago 2021, Volume 25 Nº 8 Páginas 514 - 521

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O cultivo de soja vem crescendo nos últimos anos em ambientes de solos arenosos e com risco climático, mas a produtividade é baixa, principalmente nos primeiros anos. Objetivou-se neste estudo avaliar o efeito do manejo de culturas de cobertura e nitrogênio em solo arenoso previamente ocupado por pastagem degradada na produtividade da soja. O estudo foi conduzido no Oeste de São Paulo. O delineamento experimental foi em blocos casualizados, com quatro repetições e os tratamentos foram: aveia preta; aveia preta + 50 kg ha-1 de N na aveia preta; aveia preta + 50 kg ha-1 de N na soja; aveia preta + tremoço; aveia preta + tremoço + 50 kg ha-1 de N na soja; tremoço; pousio; pousio + 50 kg ha-1 de N na soja. O teor de nitrogênio da biomassa microbiana foi maior com aveia preta + N na soja aplicado no início do florescimento (R1). O número de nódulos nas raízes da soja aumentou 2,3 vezes com aveia e aveia + N na soja em relação ao pousio. A produtividade da soja foi maior nos tratamentos com aveia + N na aveia (2.130 kg ha-1), aveia (2.038 kg ha-1) e aveia + N na soja (1.872 kg ha-1). Na ausência de culturas de cobertura, a adubação nitrogenada na soja aumentou a produtividade em 19% (262 kg ha-1) comparado ao pousio. A aveia preta é a melhor opção para aumentar a produtividade da soja. Porém na ausência de planta de cobertura é necessária a adubação nitrogenada na soja.

ABSTRACT Soybean cropping has been growing in recent years in environments with sandy soils and with climatic risk, but yield is low, especially in the early years. The objective of this study was to evaluate the effect of cover crops and nitrogen management in a sandy soil previously under degraded pastures on soybean yield. The study was conducted in Western São Paulo state, Brazil. The experimental design was randomized blocks, with four replicates, and the treatments were: black oats; black oats + 50 kg ha-1 of N in black oats; black oats + 50 kg ha-1 of N in soybean; black oats + lupine; black oats + lupine + 50 kg ha-1 of N in soybean; lupine; fallow; fallow + 50 kg ha-1 of N in soybean. Nitrogen concentration of the microbial biomass was higher with oats + N in soybean applied at the beginning of flowering (R1). The number of nodules in soybean roots increased by 2.3 times with oats and oats + N in soybean as compared to fallow. Soybean yield was higher in treatments with oats + N in oats (2,130 kg ha-1), oats (2,038 kg ha-1) and oats + N in soybean (1,872 kg ha-1). In the absence of cover crops, N fertilization in soybean increased yield by 19% (262 kg ha-1) compared to fallow. Black oats are the best option to increase soybean yield. However, in the absence of cover crops, nitrogen fertilization in soybean is necessary.

https://doi.org/10.1590/1807-1929/agriambi.v25n8p514-521 498 downloads

Mortality in patients with multidrug-resistant Pseudomonas aeruginosa infections: a meta-analysis
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Matos, Eliseth Costa Oliveira de

; Andriolo, Regis Bruni

; Rodrigues, Yan Corrêa

; Lima, Patrícia Danielle Lima de

; Carneiro, Irna Carla do Rosário Souza

; Lima, Karla Valéria Batista

Revista da Sociedade Brasileira de Medicina Tropical

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Ago 2018, Volume 51 Nº 4 Páginas 415 - 420

Resumo: En | Texto: En | PDF: En

Abstract Pseudomonas aeruginosa is the leading cause of nosocomial infections with high mortality rates owing to the limited therapeutic options for multidrug-resistant Pseudomonas aeruginosa (MDRPA) and metallo-beta-lactamase (MBL)-producing strains. Herein, we present a meta-analysis exploring the association between MDRPA and São Paulo MBL-1 (SPM-1)-producing strains vs. mortality. Online databases were screened to identify studies published between 2006 and 2016. A total of 15 studies, comprising 3,201 cases of P. aeruginosa infection, were included. Our results demonstrated a higher mortality rate among patients infected with MDRPA (44.6%, 363/813) than those with non-MDRPA infection (24.8%, 593/2,388) [odds ratio (OR) 2.39, 95% confidence interval (CI) 1.70-3.36, p <0.00001]. The risk of mortality in patients with non-SPM-1 strains was four times higher than that observed in the patients of the SPM-1 group; however, no statistically significant difference was observed (p = 0.43). In conclusion, the results of our study demonstrated that patients infected with MDRPA had a significantly higher mortality rate than that of patients infected with non-MDRPA strains, especially patients with bloodstream infection (BSI), immunosuppression, and inadequate antimicrobial therapy. The absence of studies on the molecular aspects of blaSPM-1 and its association with mortality limited the analysis; therefore, our results should be interpreted with caution. Our findings also highlight the need for more studies on the molecular aspects of resistance and the peculiarities of different nosocomial settings.

https://doi.org/10.1590/0037-8682-0506-2017 2287 downloads

Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil
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Magalhães-Matos, Paulo Cesar

; Cunha-Santos, Rafaelle

; Sousa, Paulo Geovani Silva

; Sampaio-Júnior, Francisco Dantas

; Barros, Flávia de Nazaré Leite

; Mourão, Fábio Rodrigo Paixão

; Oliveira, Wanessa Batista Lima

; Gabriel, Áurea Martins

; Monteiro, Silvia Gonzalez

; Góes-Cavalcante, Gustavo

; Scofield, Alessandra

Ciência Rural

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Abr 2016, Volume 46 Nº 4 Páginas 663 - 668

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo do presente trabalho foi realizar o diagnóstico da infecção natural por Trypanosoma evansi em procionídeos de vida livre e de cativeiro dos estados do Amapá e Pará, Brasil. Durante o período de fevereiro de 2012 a agosto de 2013, amostras de sangue total e esfregaços sanguíneos foram obtidos de 45 procionídeos de vida livre e de nove mantidos em cativeiro em mantenedores e Parques Zoobotânicos dos estados do Amapá e Pará. As amostras de sangue total foram coletadas e mantidas a -20ºC para pesquisa de DNA de T. evansi pela PCR utilizando-se os iniciadores RoTat 1.2 forward e RoTat 1.2 reverse. Os esfregaços sanguíneos também foram processados e examinados para a pesquisa de formas tripomastigotas do agente. DNA de T. evansi foi detectado em 18,52% (10/54) dos procionídeos, ocorrendo em mãos-peladas de cativeiro e quatis de vida livre e de cativeiro no estado do Pará. Não foram observadas formas tripomastigotas nos esfregaços sanguíneos. DNA de T. evansi foi detectado em P. cancrivorus e N. nasua no estado do Pará, sendo este o primeiro relato em P. cancrivorus.

ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crab-eating raccoons and captive and free-living coatis in Pará State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Pará State, being this the first such report in P. cancrivorus.

https://doi.org/10.1590/0103-8478cr20150679 3488 downloads

Antimicrobial activity of the myrsinoic acid A from Myrsine coriacea and the semi-synthetic derivatives
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Burger, Marcela Carmen de Melo

; Terezan, Ana Paula

; Cunha, Gracielle Silva de Oliveira

; Fernandes, Joao Batista

; Silva, Maria Fatima das Graças Fernandes da

; Vieira, Paulo Cezar

; Menezes, Antonio Carlos Severo

Revista Brasileira de Farmacognosia

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Out 2015, Volume 25 Nº 5 Páginas 451 - 454

Resumo: En | Texto: En | PDF: En

ABSTRACTThe antimicrobial activity of the myrsinoic acid A isolated from Myrsine coriacea (Sw.) R.Br. ex Roem. & Schult., Primulaceae, and a two semi-synthetics derivatives was tested against Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar typhi, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Micrococcus luteus, Candida albicans, Candida krusei and Candida tropicalis. The microdilution method was used for the determination of the minimum inhibitory concentration during evaluation of the antimicrobial activity. The myrsinoic acid A showed no activity against the selected microorganisms but the hydrogenated and acetylated derivatives were active against B. subtilis, E. coli, S. aureus and P. aeruginosa.

https://doi.org/10.1016/j.bjp.2015.07.023 1274 downloads

10.

Dye Degradation Enhanced by Coupling Electrochemical Process and Heterogeneous Photocatalysis
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Santos, Lidiaine M.

; Amorim, Kamila P. de

; Andrade, Leonardo S.

; Batista, Paulo S.

; Trovó, Alam G.

; Machado, Antonio E. H.

Journal of the Brazilian Chemical Society

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Set 2015, Volume 26 Nº 9 Páginas 1817 - 1823

Resumo: En | Texto: En | PDF: En

In this study, we evaluated the combination between an electrochemical process, occurring in the dark, and a heterogeneous photocatalytic process for dye degradation, using the azo dye tartrazine as model of oxidizable substrate. TiO2 P25 and an Ag-doped TiO2 were used as photocatalysts in suspensions containing 50 mg L–1 of tartrazine. The best result, 74% of dye mineralization in 120 min of reaction, was obtained using TiO2 P25 as photocatalyst and a current density of 10 mA cm–2 in the electrochemical cell, a value 30% higher than the sum of the results obtained by heterogeneous photocatalysis (44%) and electrochemical oxidation (13%). The use of Ag-doped TiO2 did not result in significant improvement on tartrazine mineralization, due to the aggregation of these nanoparticles. Our results suggest that this process can be an alternative for a complete treatment (discoloration and mineralization) of tartrazine and most likely other azo dyes.

https://doi.org/10.5935/0103-5053.20150158 2244 downloads

11.

Partial characterization of Plasmodium falciparum trophozoite proteome under treatment with quinine, mefloquine and the natural antiplasmodial diosgenone
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Segura, César

; Cuesta-Astroz, Yesid

; Nunes-Batista, Camila

; Zalis, Mariano

; von Krüger, Wanda Maria de Almeida

; Mascarello Bisch, Paulo

Biomédica

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Jun 2014, Volume 34 Nº 2 Páginas 237 - 249

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Introducción. A pesar de los esfuerzos para controlar la malaria, esta sigue siendo un problema de salud pública. Plasmodium falciparum es responsable de la mayoría de los casos graves y de las muertes. Los programas de control de la malaria han sido cuestionados debido al fracaso del tratamiento y a la resistencia del parásito a los antipalúdicos de primera línea, por lo que se requieren nuevas y mejores alternativas. El análisis proteómico permite identificar y determinar los niveles de expresión de las proteínas bajo la presión de los medicamentos, lo que posibilita la identificación de nuevos blancos terapéuticos y mecanismos de acción. Objetivo. Analizar cualitativamente la expresión diferencial de proteínas del citosol del trofozoíto de P. falciparum bajo tratamiento con quinina, mefloquina y el compuesto natural diosgenona mediante una aproximación proteómica. Materiales y métodos. Se trataron trofozoítos sincronizados y cultivados in vitro de P. falciparum (cepa ITG2) con quinina, mefloquina y el compuesto natural diosgenona. Los extractos proteicos se prepararon y analizaron por electroforesis bidimensional. Las proteínas con aparente expresión diferencial se seleccionaron e identificaron mediante espectrometría de masas MALDI-TOF. Resultados. Se encontraron las siguientes proteínas diferencialmente expresadas en el trofozoíto: la enolasa (PF10_0155), la proteína de unión a calcio (PF11_0098), la chaperonina (PFL0740c), la proteína de invasión a la célula del huésped (PF10_0268) y la proteína relacionada con procesos de reducción y oxidación (redox) (MAL8P1.17). Estos hallazgos son congruentes con resultados previos de estudios en los que el parásito fue presionado con otros medicamentos antipalúdicos. Conclusión. Los cambios observados en el perfil de proteínas del trofozoíto de P. falciparum tratado con antipalúdicos involucraron preferencialmente proteínas relacionadas con la respuesta al estrés general.

Introduction: Despite efforts to control malaria, around 10% of the world population is at risk of acquiring this disease. Plasmodium falciparum accounts for the majority of severe cases and deaths. Malaria control programs have failed due to the therapeutic failure of first-line antimalarials and to parasite resistance. Thus, new and better therapeutic alternatives are required. Proteomic analysis allows determination of protein expression levels under drug pressure, leading to the identification of new therapeutic drug targets and their mechanisms of action. Objective: The aim of this study was to analyze qualitatively the expression of P.falciparum trophozoite proteins (strain ITG2), after exposure to antimalarial drugs, through a proteomic approach. Materials and methods: In vitro cultured synchronized parasites were treated with quinine, mefloquine and the natural antiplasmodial diosgenone. Protein extracts were prepared and analyzed by two-dimensional electrophoresis. The differentially expressed proteins were selected and identified by MALDI-TOF mass spectrometry. Results: The following proteins were identified among those differentially expressed in the parasite in the presence of the drugs tested: enolase (PF10_0155), calcium-binding protein (PF11_0098), chaperonin (PFL0740c), the host cell invasion protein (PF10_0268) and proteins related to redox processes (MAL8P1.17). These findings are consistent with results of previous studies where the parasite was submitted to pressure with other antimalarial drugs. Conclusion: The observed changes in the P. falciparum trophozoite protein profile induced by antimalarial drugs involved proteins mainly related to the general stress response.

https://doi.org/10.7705/biomedica.v34i2.1700 1702 downloads

12.

Breeding of Biomphalaria tenagophila in mass scale
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Rosa, Florence Mara

; Marques, Daisymara P. Almeida

; Maciel, Engels

; Couto, Josiane Maria

; Negrão-Corrêa, Deborah A.

; Teles, Horácio M. Santana

; Santos, João Batista dos

; Coelho, Paulo Marcos Zech

Revista do Instituto de Medicina Tropical de São Paulo

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Fev 2013, Volume 55 Nº 1 Páginas 39 - 44

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Foi desenvolvido um método eficiente de criação em larga escala de Biomphalaria tenagophila (linhagem Taim/RS) durante o período de 2005-2010. Foi concebida uma instalação que consiste de quatro tanques de alvenaria (9,4 x 0,6 x 0,22) com fundos recobertos por uma mistura constituída de terra vermelha esterilizada e carbonato de cálcio. Foi padronizado que cada tanque de criação conteria em média 3.000 exemplares e receberia diariamente 35.000 mg de ração e alface como complemento. O desenvolvimento de um sistema de aquecimento por efeito estufa constituído de lonas escuras móveis permitiu controlar a temperatura entre 20 a 24 ºC, sistema essencial principalmente nos meses mais frios. Durante o período de 14 meses foram produzidos aproximadamente 27.000 exemplares com diâmetros superiores a 12 mm. As taxas de mortalidade dos caramujos recém-eclodidos e adultos foram de 77% e 37%, respectivamente. O acompanhamento do ritmo de crescimento de 310 B. tenagophila demonstrou que caramujos com 70 dias de idade alcançaram em média 17,0 ± 0,9 mm de diâmetro. As taxas de mortalidade e o desempenho de crescimento de caramujos do gênero B. tenagophila podem ser considerados altamente satisfatórios, comparando-se com os resultados da literatura realizados com espécies do gênero Biomphalaria em condições controladas de laboratório.

An efficient method for breeding Biomphalaria tenagophila (Taim lineage/RS) was developed over a 5-year-period (2005-2010). Special facilities were provided which consisted of four cement tanks (9.4 x 0.6 x 0.22 m), with their bottom covered with a layer of sterilized red earth and calcium carbonate. Standard measures were adopted, as follows: each tank should contain an average of 3000 specimens, and would be provided with a daily ration of 35,000 mg complemented with lettuce. A green-house effect heating system was developed which constituted of movable dark canvas covers, which allowed the temperature to be controlled between 20 - 24 ºC. This system was essential, especially during the coldest months of the year. Approximately 27,000 specimens with a diameter of 12 mm or more were produced during a 14-month-period. The mortality rates of the newly-hatched and adult snails were 77% and 37%, respectively. The follow-up of the development system related to 310 specimens of B. tenagophila demonstrated that 70-day-old snails reached an average of 17.0 ± 0.9 mm diameter. The mortality rates and the development performance of B. tenagophila snails can be considered as highly satisfactory, when compared with other results in literature related to works carried out with different species of the genus Biomphalaria, under controlled laboratory conditions.

https://doi.org/10.1590/S0036-46652013000100007 8776 downloads

13.

Evaluation of impacts of climate change and local stressors on the biotechnological potential of marine macroalgae: a brief theoretical discussion of likely scenarios
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Horta, Paulo A.

; Vieira-Pinto, Talita

; Martins, Cintia D. L.

; Sissini, Marina N.

; Ramlov, Fernanda

; Lhullier, Cintia

; Scherner, Fernando

; Sanches, Paola F.

; Farias, Julyana N.

; Bastos, Eduardo

; Bouzon, Janayna L.

; Munoz, Pamela

; Valduga, Eduardo

; Arantes, Noele P.

; Batista, Manuela B.

; Riul, Pablo

; Almeida, Rafael S.

; Paes, Eduardo

; Fonseca, Alessandra

; Schenkel, Eloir P.

; Rorig, Leonardo

; Bouzon, Zenilda

; Barufi, José Bonomi

; Colepicolo, Pio

; Yokoya, Nair

; Copertino, Margareth S.

; Oliveira, Eurico C. de

Revista Brasileira de Farmacognosia

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Ago 2012, Volume 22 Nº 4 Páginas 768 - 774

Resumo: En | Texto: En | PDF: En

Climate change can be associated with variations in the frequency and intensity of extreme temperatures and precipitation events on the local and regional scales. Along coastal areas, flooding associated with increased occupation has seriously impacted products and services generated by marine life, in particular the biotechnological potential that macroalgae hold. Therefore, this paper analyzes the available information on the taxonomy, ecology and physiology of macroalgae and discusses the impacts of climate change and local stress on the biotechnological potential of Brazilian macroalgae. Based on data compiled from a series of floristic and ecological works, we note the disappearance in some Brazilian regions of major groups of biotechnological interest. In some cases, the introduction of exotic species has been documented, as well as expansion of the distribution range of economically important species. We also verify an increase in the similarities between the Brazilian phycogeographic provinces, although they still remain different. It is possible that these changes have resulted from the warming of South Atlantic water, as observed for its surface in southeastern Brazilian, mainly during the winter. However, unplanned urbanization of coastal areas can also produce similar biodiversity losses, which requires efforts to generate long-term temporal data on the composition, community structure and physiology of macroalgae.

3337 downloads

14.

Novas N-benzoiltiraminas de Swinglea glutinosa (Rutaceae)
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Cerqueira, Cristovam do Nascimento

; Santos, Djalma A. P. dos

; Malaquias, Karla da Silva

; Lima, Murilo Marinho de Castro

; Silva, Maria Fátima das Graças Fernandes da

; Fernandes, João Batista

; Vieira, Paulo Cezar

Química Nova

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2012, Volume 35 Nº 11 Páginas 2181 - 2185

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Phytochemical studies of the leaves and fruits have led to the identification of the known amides (E)-N-methyl-cinnamamide, N-benzoyltyramine, N-benzoyl-O-geranyltyramine, N-benzoyl-O-(4-acetoxyl)-geranyltyramine, in addition to the new N-{2-[4-(butoxyl-3-one)phenyl]ethyl}benzamide, N-{2-[4-(2,3-dihydroxy-2-methyl-butoxylanal)phenyl]ethyl}benzamide, N-{2-[4-(2,3-dihydroxy-2-methyl-butoxyloic)phenyl]ethyl}benzamide, N-benzoyl-O-(4-acetoxyl-6,7-epoxy)-geranyltyramine, N-benzoyl-O-(4-acetoxyl-6,7-dihydroxy)-geranyltyramine and N-benzoyl-O-(6-acetoxyl-4,7-dihydroxy)-geranyltyramine. The isolated compounds clearly point to Swinglea phytochemical affinities with other Aurantioideae species.

2947 downloads

15.

Ecological distribution of the shrimp Pleoticus muelleri (Bate, 1888) and Artemesia longinaris Bate, 1888 (Decapoda, Penaeoidea) in the southeastern Brazilian littoral
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Batista, Abner Carvalho

; Simões, Sabrina Morilhas

; Lopes, Mateus

; Costa, Rogerio Caetano da

Nauplius

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Dez 2011, Volume 19 Nº 2 Páginas 135 - 143

Resumo: En | Texto: En | PDF: En

The relationship between species abundance and environmental factors such as the bottom water temperature and salinity, the texture and organic matter content of the sediment and the distribution of Pleoticus muelleri and Artemesia longinaris were investigated. Specimens and samples of abiotic factors were collected monthly from May 2008 to April 2010 at 4 locations in Santos Bay on the southern coast of the State of São Paulo. A shrimp boat equipped with an otter-trawl net with an 8 m mouth aperture and a mesh size of 20 mm tapering to 18 mm at the cod end was used for sampling. Shrimp abundances were compared with an analysis of variance (ANOVA). However, when the data did not follow a normal distribution, we used the Kruskal-Wallis test. The relationship between environmental factors and the abundance of individuals was assessed with a Pearson's correlation. The largest catches of individuals of both species occurred in the late spring of 2008. The greatest abundance occurred at the outer part of the bay (collection point 4). The greatest abundance of P. muelleri was associated with lower temperatures and sediments with higher clay and organic matter content, whereas for A. longinaris, there was no significant correlation with any of the abiotic factors recorded. However, the occurrence of both species was related to lower bottom temperatures associated with the intrusion of the South Atlantic Central Water in the region.

1850 downloads

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