Resumo Este estudo investigou os efeitos da aplicação de plasma não térmico à pressão atmosférica (PLAR) e da reidratação dentinária com água (REID) na resistência de união (RU) de adesivos. Foram testados três adesivos convencionais: Scotchbond Multi-Purpose (SBM / primer à base de água + resina adesiva), Gluma Bond Universal (GBU / frasco único contendo acetona como solvente orgânico) e Prime&Bond Universal (PBU / frasco único contendo propanol como solvente orgânico). Os adesivos foram aplicados: 1- na dentina condicionada com ácido fosfórico (Controle), 2- após aplicação de PLAR por 45 segundos na dentina condicionada ou 3- após REID com água (10 segundos) na dentina condicionada tratada com plasma. A RU foi realizada utilizando molares humanos e teste de microtração, em que as amostras foram testadas após 24 horas ou um ano (n=6). Os dados de RU foram analisados por ANOVA mista de três fatores e teste de Bonferroni (α=0,05). A morfologia da dentina condicionada (n=3) e as interfaces dentina-adesivo (n=3) foram avaliadas por MEV. PLAR e REID não aumentaram a RU da GBU e PBU. A RU do SBM para a dentina condicionada não tratada com NAPP (Controle) reduziu significativamente após um ano. O adesivo PBU apresentou maior resistência de união à dentina quando os adesivos testados foram aplicados após REID. O ácido fosfórico desmineralizou a dentina até uma profundidade de 5 mm que permitiu a penetração do adesivo nos túbulos dentinários, formando tags de resina. As estruturas interfaciais foram mantidas após um ano. O tratamento com plasma de argônio não foi benéfico no aumento da resistência de união dentinária dos adesivos à dentina condicionada em 24 horas. Entretanto, a reidratação após a aplicação do plasma evitou a redução da resistência de união do adesivo de três etapas após um ano. (PLAR (REID (RU convencionais MultiPurpose Multi Purpose adesiva, adesiva , adesiva) orgânico PrimeBond Prime orgânico. . 1 Controle, Controle 2 4 3 10 (1 microtração n=6. n6 n n=6 6 (n=6) α=0,05. α005 α α=0,05 0 05 (α=0,05) n=3 n3 (n=3 dentinaadesivo MEV (Controle dentinários Entretanto ( n= (n=6 α00 α=0,0 (α=0,05 (n= α0 α=0, (α=0,0 (n α=0 (α=0, α= (α=0 (α= (α

Abstract This study investigated the effects of nonthermal atmospheric pressure plasma (NAPP) application and dentin rehydration with water (REHY) on bond strength (BS) of adhesives. Three etch-and-rinse adhesives were tested: Scotchbond Multi-Purpose (SBM / water-based primer + adhesive resin), Gluma Bond Universal (GBU / single-bottle containing acetone as organic solvent) and Prime&Bond Universal (PBU / single-bottle containing propanol as organic solvent). Adhesives were applied: 1- to phosphoric acid-etched dentin (Control), 2- after NAPP application for 45 seconds to etched dentin or 3- after REHY with water (10 seconds) of plasma-treated etched dentin. BS was performed using human molars and microtensile test, in which specimens were tested after 24 hours or one year (n=6). BS data were analyzed by three-way mixed ANOVA and Bonferroni’s test (α=0.05). The morphology of etched dentin (n=3) and the dentin-adhesive interfaces (n=3) were evaluated using SEM. NAPP and REHY did not increase the BS of GBU and PBU. The BS of SBM to etched dentin not treated with NAPP (Control) reduced significantly after one year. The PBU adhesive showed the highest bond strength to dentin when the adhesives tested were applied after REHY. Phosphoric acid demineralized dentin to a depth of 5 mm that allowed the adhesive penetration into the dentinal tubules, forming the resin tags. Interfacial structures were maintained after one year. Argon plasma treatment was not beneficial in increasing the dentin bond strength of adhesives to etched-dentin at 24 hours. However, rehydration after plasma application prevented the bond strength reduction for three-step adhesive after one year. (NAPP (REHY (BS etchandrinse etch rinse MultiPurpose Multi Purpose waterbased based resin, , resin) singlebottle single bottle solvent PrimeBond Prime solvent. . 1 acidetched Control, Control 2 4 3 10 (1 plasmatreated n=6. n6 n n=6 6 (n=6) threeway three way Bonferronis Bonferroni s α=0.05. α005 α α=0.05 0 05 (α=0.05) n=3 n3 (n=3 dentinadhesive SEM (Control tubules tags etcheddentin However threestep step ( n= (n=6 α00 α=0.0 (α=0.05 (n= α0 α=0. (α=0.0 (n α=0 (α=0. α= (α=0 (α= (α

Abstract: The aim of the study was to evaluate how the association of solvents (tetrahydrofuran [THF], dimethyl sulfoxide [DMSO], ethanol [ET] or acetone [ACT]) with experimental dental adhesives affects selected properties of experimental dental adhesives and dentin bond durability. Six adhesive combinations were prepared containing: 30 % ET, 30 % ACT, 30 % THF, 28 % ET + 2 % DMSO (ET+DMSO), 15 % ethanol + 15 % THF (ET+THF), or 28 % THF + 2 % DMSO (THF+DMSO). Thirty-six molars (n = 6) were cut to expose the coronary dentin, and were randomly divided according to the adhesives. They were restored, and then cut into resindentin sticks (1 mm²), and stored in distilled water for 24 h or 6 months, until conducting the microtensile bond strength and nanoleakage tests. Other experiments performed with adhesives included viscosity assessment using a rheometer, and degree of conversion using Fourier-transform infrared spectroscopy (FTIR). The data were analyzed statistically using two-way ANOVA and Tukey’s test (p < 0.05). The adhesive formulated exclusively with THF showed the highest viscosity, followed by ET+THF, which obtained the highest degree of conversion compared to ET, and THF alone. ET+DMSO obtained the highest 24-h and aged bond strengths (p < 0.05). ET+THF increased the nanoleakage slightly after 6 months, but attained the only gap-free adhesive interface among all the groups. The combination of alternative solvents, particularly THF, with conventional ones (ET) has improved chemical properties, and the dentin bonding of experimental simplified adhesives.