Groundwater is considered an important source of water aid for human consumption. However, this water can be contaminated by impurities, such contamination spots that might reach the water tables. The aim of this study was to investigate the quality of shallow groundwater in Flores neighborhood, located in the municipality of Benevides, within Belém metropolitan region, State of Pará, Brazil. Recommendations described in Standard Methods for Examination of Water and Wastewater were followed at the time to determine physical-chemical parameters such as potential hydrogen (pH), electrical conductivity (EC), total dissolved solids (TDS), apparent color, turbidity, total hardness (TH), ions and trace elements. Multivariate statistical analysis has shown that most predictive water-quality parameters set for human consumption are associated with natural events involving weathering and leaching processes. Nevertheless, parameters of nitrate, phosphate, EC, TDS and pH are also influenced by anthropic conditions in the surroundings, mainly phosphate and nitrate ions. Therefore, it can be concluded that shallow groundwaters in Flores neighborhood are unsuitable for human consumption, making it necessary for municipal public authorities to take action to solve the problem of water shortage in the borough, thus preventing the population from use those shallow groundwaters, as their consumption can bring health problems. However impurities tables Benevides region Pará Brazil physicalchemical physical chemical pH, , (pH) EC (EC) TDS, (TDS) color turbidity TH, TH (TH) elements waterquality processes Nevertheless surroundings Therefore borough problems (pH (EC (TDS (TH

The use of materials such as organofunctionalized silica for application in solid phase extraction (SPE) allows the preconcentration of several ions, including Pt, and subsequent detection. This work aims to synthesize and characterize an organofunctionalized silica (Sil-TMSDT) with the silylant N’(trimethoxysilyl)propylethylenetriamine and its application as a solid phase in the preconcentration Pt4+ ions in aqueous media, using detection by flame atomization atomic absorption spectroscopy (FAAS). The Fourier-transform infrared spectroscopy (FTIR) spectra and scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS) confirmed the aminofunctionalization of the silica. For the adsorption process, a preconcentration mini-column with 200 mg of Sil-TMSDT, a solution of 0.485 mg L−1 of K2[PtCl6] at pH 10 and HNO3 0.5 mol L−1 as eluent were used, obtaining an enrichment factor of 15-fold with retention efficiency higher than 99%. The Pt4+ ion determined by FAAS showed a linear response between 0.02 and 0.40 mg L−1; a linear coefficient where R = 0.9981; a 16-fold preconcentration factor; a limit of quantification (LOQ) and limit of detection (LOD) of 0.045 and 0.014 mg L−1, respectively. The proposal system showed analytical applicability to determining Pt4+ ion in an aqueous medium and could be used to detect traces of this potentially toxic metal in biological fluids such as urine and blood. SPE (SPE Pt SilTMSDT Sil TMSDT (Sil-TMSDT Ntrimethoxysilylpropylethylenetriamine N trimethoxysilyl propylethylenetriamine Pt4 media FAAS. . (FAAS) Fouriertransform Fourier transform FTIR (FTIR SEM (SEM energydispersive energy dispersive EDS (EDS process minicolumn mini column 20 SilTMSDT, TMSDT, Sil-TMSDT 0485 0 485 0.48 L1 L 1 L− K2PtCl6 KPtCl K2 PtCl6 K PtCl K2[PtCl6 HNO 05 5 0. 15fold fold 15 99 99% 002 02 0.0 040 40 0.4 0.9981 09981 9981 16fold 16 LOQ (LOQ LOD (LOD 0045 045 0.04 0014 014 0.01 respectively blood (FAAS 2 048 48 K2PtCl K2[PtCl 9 00 04 4 0.998 0998 998 004 001 01 0.99 099 0.9 09

ABSTRACT In Brazil, the lima bean is the second most economically significant legume within the genus Phaseolus. Climate change, particularly water scarcity, threatens the production of this species. The application of salicylic acid has mitigated the adverse effects of stress. This study aimed to assess the impact of salicylic acid on acclimatisation to water restriction in three genotypes of Phaseolus lunatus (‘Cara Larga’, ‘Cearense’, and ‘Orelha de Vó’). A completely randomised design with a triple factorial included three broad bean genotypes, two pre-conditionings with 1.0 mM salicylic acid and without this elicitor (0.0 mM), and three levels of water availability (75, 50, and 25%), totalling 18 treatments with eight replicates. Physiological and biochemical responses were evaluated after 60 days of treatment. The responses varied among the genotypes. ‘Cara Larga’ stood out regarding osmoregulatory and antioxidant parameters compared to the other genotypes. In contrast, ‘Cearense’ showed an increase only in carbohydrates and carotenoids concentrations, while ‘Orelha de Vó’ exhibited more efficient water use and higher levels of proline under greater water restriction, concurrently with a decline in other parameters. Overall, the ‘Cara Larga’ genotype appears to be the most responsive to the modulating effects induced by acid application, especially under a water restriction of 25%. Applying, applying salicylic acid under conditions of low water availability may be a strategy for modulating the synthesis of osmoregulatory and antioxidant responses in P. lunatus. Brazil change scarcity species stress Cara Larga, Larga , ‘Cearense, Cearense ‘Cearense Orelha Vó. Vó . Vó’) preconditionings pre conditionings 10 1 0 1. 0.0 00 (0. mM, mM) 75, 75 (75 50 25%, 25 25% 25%) replicates 6 treatment contrast concentrations Overall Applying P 0. (0 7 (7 5 2 (

RESUMO No Brasil, o feijão-fava é a segunda leguminosa mais significativa economicamente dentro do gênero Phaseolus. As alterações climáticas, especialmente a escassez de água, ameaçam a produção desta espécie. Neste contexto a aplicação de ácido salicílico mitigou os efeitos adversos do estresse. Este estudo teve como objetivo avaliar o impacto do ácido salicílico na aclimatação à restrição hídrica em três genótipos de Phaseolus lunatus (‘Cara Larga’, ‘Cearense’ e ‘Orelha de Vó’). O delineamento inteiramente casualizado com fatorial triplo incluiu três genótipos de fava, dois pré-condicionamentos com ácido salicílico 1,0 mM e sem esse elicitor (0,0 mM) e três níveis de disponibilidade hídrica (75, 50 e 25%), totalizando 18 tratamentos com oito repetições. As respostas fisiológicas e bioquímicas foram avaliadas após 60 dias de tratamento. As respostas variaram entre os genótipos. ‘Cara Larga’ destacou-se nos parâmetros osmorregulatórios e antioxidantes em relação aos demais genótipos. Em contrapartida, ‘Cearense’ apresentou aumento apenas nas concentrações de carboidratos e carotenoides, enquanto ‘Orelha de Vó’ apresentou uso mais eficiente da água e maiores teores de prolina sob maior restrição hídrica, concomitantemente com declínio em outros parâmetros. No geral, o genótipo ‘Cara Larga’ parece ser o mais responsivo aos efeitos moduladores induzidos pela aplicação de ácido, especialmente sob restrição hídrica de 25%. A aplicação, a aplicação de ácido salicílico em condições de baixa disponibilidade hídrica pode ser uma estratégia para modular a síntese de respostas osmorreguladoras e antioxidantes em P. lunatus. Brasil feijãofava feijão fava climáticas espécie estresse Cara Larga, Larga , ‘Cearense Cearense Orelha Vó. Vó . Vó’) précondicionamentos pré condicionamentos 10 1 0 1, 0,0 00 (0, 75, 75 (75 5 25%, 25 25% 25%) repetições 6 tratamento destacouse destacou se contrapartida carotenoides geral P 0, (0 7 (7 2 (

ABSTRACT Salt stress hampers the growth and physiology of nasturtium (Tropaeolum majus), due to biochemical, physiological, and anatomical disruptions. The application of salicylic acid stands as an alternative to alleviate the detrimental effects of salt stress, but studies on nasturtium are scarce. Thus, the aim of present study was to assess the effects of foliar application of salicylic acid on nasturtium cultivated under salt stress. The experiment followed a completely randomized design in a 3 x 3 factorial scheme, with 0 (no stress), 50 (moderate salt stress), and 100 (severe salt stress) mM of NaCl, and application of 0, 0.5, and 1 mM of salicylic acid, each with six replications. Growth (plant height, stem diameter, and number of leaves), gas exchange (stomatal conductance, photosynthesis, transpiration, internal CO2 concentration, intrinsic water use efficiency, instantaneous water use efficiency, and intrinsic carboxylation efficiency), as well as chlorophyll indices and chlorophyll a fluorescence were evaluated. Salt stress affected the variables analyzed in this study. The application of salicylic acid had a positive effect on mitigating the effects of severe salt stress, resulting in a significant increase in the number of leaves. The most effective dose was 1 mM, also leading to notable improvements in water use efficiency and photochemical efficiency. However, other combinations of salinity and salicylic acid reduced growth and gas exchange in nasturtium plants. Tropaeolum majus, majus , majus) biochemical physiological disruptions scarce Thus scheme no 5 moderate 10 NaCl 05 0.5 replications plant height diameter leaves, leaves leaves) stomatal conductance photosynthesis transpiration CO concentration efficiency) evaluated However plants 0.

RESUMO O estresse salino limita o crescimento e a fisiologia da capuchinha (Tropaeolum majus), devido a distúrbios bioquímicos, fisiológicos e anatômicos. A aplicação de ácido salicílico é uma alternativa para mitigar os efeitos prejudiciais do estresse salino, no entanto, em capuchinha os estudos são escassos. Assim, objetivou-se avaliar os efeitos da aplicação de ácido salicílico via foliar em capuchinha cultivada sob estresse salino. O experimento foi realizado em delineamento inteiramente casualizado, em esquema fatorial 3 x 3, referente a 0 (sem estresse) 50 (estresse salino moderado) e 100 (estresse salino severo) mM de NaCl e aplicação de 0, 0,5 e 1 mM de ácido salicílico, com seis repetições. O crescimento (altura de planta, diâmetro do caule e número de folhas), trocas gasosas (condutância estomática, fotossíntese, transpiração, concentração interna de CO2, eficiência intrínseca do uso da água, eficiência instantânea do uso da água e eficiência intrínseca de carboxilação) e os índices de clorofila e fluorescência da clorofila a foram avaliados. O estresse salino afetou as variáveis analisadas neste estudo. A aplicação de ácido salicílico teve um efeito positivo na mitigação dos efeitos do estresse salino severo, resultando em um aumento significativo no número de folhas, sendo a dose mais eficaz a concentração de 1 mM, levando ainda, a melhorias notáveis na eficiência no uso da água e na eficiência fotoquímica. No entanto, as demais combinações de salinidade e ácido salicílico reduziram o crescimento e as trocas gasosas nas plantas de capuchinha. Tropaeolum majus, majus , majus) bioquímicos anatômicos entanto escassos Assim objetivouse objetivou se casualizado sem 5 moderado 10 severo 05 repetições altura planta folhas folhas) condutância estomática fotossíntese transpiração CO2 CO carboxilação avaliados estudo ainda fotoquímica

Resumo Os sabonetes têm um papel importante para a nossa higiene e saúde, pois eles além de ter efeito bactericida, também removem as sujeiras presentes no corpo humano. Avaliar a eficácia de sabonetes com atividade antimicrobiana de diferentes marcas comerciais vendidas no Brasil. Foram realizados ensaios da atividade antimicrobiana dos diferentes sabonetes através do método difusão em ágar frente aos microrganismos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Acinetobacter baumannii, Proteus mirabilis e Candida albicans. Todos os sabonetes comerciais testados apresentaram formação de halo de inibição antimicrobiana frente às bactérias S. aureus e P. aeruginosa. Apenas dois sabonetes comerciais apresentaram inibição das espécies A. baumannii e C. albicans. Nenhum dos sete produtos estudados apresentou inibição das bactérias E. cloacae, P. mirabilis e E. coli. Na comparação das informações contidas nas embalagens dos produtos com os resultados obtidos durante a pesquisa realizada foram observadas divergências. Os sabonetes que apresentaram maior eficiência contra os microrganismos testados foram aqueles das apresentações 1 e 2, que se mostraram eficazes contra as espécies de bactérias S. aureus, P. aeruginosa e A. baumannii e contra a espécie de fungo C. albicans. As marcas 3, 4, 5, 6 e 7 apresentaram o mesmo resultado de sensibilidade frente as bactérias das espécies S. aureus e P. aeruginosa, com variação quantitativa apenas do halo de inibição. Houve divergência entre as informações contidas nas embalagens dos sete produtos em estudo e os resultados dos testes experimentais.

Abstract Soaps play an important role in our hygiene and health, as they not only have a bactericidal effect but also remove dirt from the human body. To evaluate the effectiveness of soaps with antimicrobial activity from different commercial brands sold in Brazil. Tests of the antimicrobial activity of different soaps were carried out through diffusion in agar against the microorganisms Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Acinetobacter baumannii, Proteus mirabilis, and Candida albicans. All commercial soaps tested transfer antimicrobial inhibition halo formation against S. aureus and P. aeruginosa bacteria. Only two commercial soaps inhibit the species A. baumannii and C. albicans. None of the seven products studied showed inhibition of E. cloacae, P. mirabilis, and E. coli bacteria. When comparing the information contained in the packaging of the products with the results obtained during a survey, divergences were observed. The soaps that provide greater efficiency against the tested microorganisms were presented in presentations 1 and 2, which become useful against the bacteria species S. aureus, P. aeruginosa, and A. baumannii and a fungus species C. albicans. Marks 3, 4, 5, 6, and 7 parallel the same sensitivity result opposite as bacteria of the species S. aureus and P. aeruginosa, with quantitative variation only of the inhibition halo. There was a divergence between the information contained in the packaging of the seven products under study and the results of the experimental tests.

Resumo Em uma escala global o Sorghum bicolor (L.) é uma das culturas mais importantes, ocupando o quinto lugar entre todos os cereais. É cultivada na África, Ásia, Oceania e Américas, onde serve como fonte de alimento e ração para humanos e animais, respectivamente. No entanto, a produção é limitada por vários fatores, incluindo pragas e doenças. Entre as pragas que atacam o sorgo, Schizaphis graminum (Rondani), comumente chamado de pulgão verde dos cereais, é a praga mais destrutiva do sorgo. O dano de S. graminum ao sorgo é agravado pelo déficit hídrico que favorece sua ocorrência. Informações limitadas sobre a distribuição espacial da praga e seus inimigos naturais impedem o desenvolvimento de estratégias de manejo ecologicamente corretas. Portanto, o objetivo desta pesquisa foi determinar a distribuição espaço-temporal de S. graminum e seu inimigo natural Coccinella septempunctata (L.) durante a fase vegetativa e reprodutiva da cultura por meio de análise geoestatística. A distribuição espaço-temporal de S. graminum foi influenciada pelo estágio de desenvolvimento do sorgo, paisagem circundante e presença de seu principal predador C. septempunctata. Além disso, a abundância de C. septempunctata foi influenciada pela densidade de S. graminum. Os resultados deste estudo são necessários para o desenvolvimento de estratégias sustentáveis de manejo de pragas contra S. graminum.

Abstract At the global level, Sorghum bicolor (L.), is one of the most important crops, which ranks fifth among all cereals. It is cultivated in Africa, Asia, Oceania and the Americas, where it serves as a source of food and feed for humans and animals, respectively. However, production is constrained by several factors including pests and diseases. Among the pests that are known to attack Sorghum, Schizaphis graminum (Rondani), commonly called the green cereal aphid, is the most destructive pest of sorghum. S. graminum damage to sorghum is worsen by water deficit which favors its occurrence. Limited information on the spatial distribution of the pest and its natural enemies impedes the development of ecologically friendly management strategies. Therefore, the objective of this research was to determine the spatiotemporal distribution of S. graminum and its natural enemy Coccinella septempunctata (L.) during the vegetative and reproductive stage of the crop using geostatistical analysis. The spatiotemporal distribution of S. graminum was influenced by Sorghum development stage, surrounding landscape, and presence of its main predator C. septempunctata. Moreover, the abundance of C. septempunctata was influenced by the density of S. graminum. The findings from this study are required for developing sustainable pest management strategies against S. graminum.

Resumo Os microRNAs (miRNAs) são reconhecidos como biomarcadores do diabetes mellitus tipo 2 (DM2), úteis para a compreensão do metabolismo da doença, e possuem grande potencial como alvos terapêuticos. O aumento da expressão de BDNF e IGF1 está altamente envolvido nos benefícios as vias de insulina e glicose, porém, são regulados negativamente em condições de resistência à insulina, enquanto seu aumento de expressão está correlacionado com a melhora do metabolismo da glicose e da insulina. Estudos sugerem a regulação desses genes por microRNA em vários contextos diferentes, proporcionando uma nova abordagem de investigação para compreender o metabolismo do DM2 e revelar potenciais alvos terapêuticos. No presente estudo, investigamos em diferentes modelos animais (humanos, ratos e camundongos) miRNAs que têm como alvo BDNF e IGF1 em tecido muscular esquelético com condições fisiológicas de DM2. As análises foram realizadas utilizando ferramentas de bioinformática e bancos de dados para predição de miRNA, homologia molecular, validação experimental de interações, expressão na condição fisiológica estudada e interação em rede. Os resultados mostraram três candidatos a miRNAs para IGF1 (miR-29a, miR-29b e miR-29c) e um para BDNF (miR-206). As avaliações experimentais e a busca pela expressão no músculo esquelético de indivíduos com DM2 confirmaram a interação prevista entre miRNA-mRNA para miR-29b e miR-206 através de modelos humanos, ratos e camundongos. Essa interação foi reafirmada em múltiplas análises de rede. Em conclusão, nossos resultados mostram a relação de regulação entre miR-29b e miR-206 com os genes investigados, em diversos tecidos, sugerindo um padrão de inibição. Contudo, esses dados mostram um grande número de possíveis processos fisiológicos de interação para perspectivas biotecnológicas.

Abstract microRNAs (miRNAs) are recognized as diabetes mellitus type 2 (T2DM) biomarkers useful for disease metabolism comprehension and have great potential as therapeutics targets. BDNF and IGF1 increased expression are highly involved in the benefits of insulin and glucose paths, however, they are down-regulated in insulin resistance conditions, while their expression increase is correlated to the improvement of glucose and insulin metabolism. Studies suggest the microRNA regulation of these genes in several different contexts, providing a novel investigation approach for comprehending T2DM metabolism and revealing potential therapeutic targets. In the present study, we investigate in different animal models (human, rat, and mouse) miRNAs that target BDNF and IGF1 in skeletal muscle tissue with T2DM physiological conditions. Bioinformatics tools and databases were used to miRNA prediction, molecular homology, experimental validation of interactions, expression in the studied physiological condition, and network interaction. The findings showed three miRNAs candidates for IGF1(miR-29a, miR-29b, and miR-29c) and one for BDNF (miR-206). The experimental evaluations and the search for the expression in skeletal muscle from T2DM subjects confirmed the predicted interaction between miRNA-mRNA for miR-29b and miR-206 through human, rat, and mouse models. This interaction was reaffirmed in multiple network analyses. In conclusion, our results show the regulation relationship between miR-29b and miR-206 with the investigated genes, in several tissues, suggesting an inhibition pattern. Nevertheless, these data show a large number of possible interaction physiological processes, for future biotechnological prospects.

RESUMEN Introducción: El VIH/SIDA es considerado uno de los grandes casos de salud pública, sin embargo, está comprobado que pacientes que hacen uso de la terapia antirretroviral (TARV) y practican entrenamiento de fuerza provoca una promoción de su salud. Objetivos: Evaluar el impacto del entrenamiento de fuerza en la resistencia a las citoquinas y en la composición corporal de las personas que viven con VIH/SIDA. Métodos: Ensayo clínico aleatorizado, la muestra estuvo compuesta por 12 pacientes, siete del Grupo de Fuerza (TFG) y cinco del Grupo de Resistencia Muscular (GRM). Se compararon los niveles de las citocinas IL-2, IL-4, IL-6, IL-10 y TNF-α y la composición corporal en la primera y la última sesión. Los pacientes completaron 36 sesiones de entrenamiento de fuerza y resistencia durante 12 semanas. Resultados: Tras 36 sesiones de entrenamiento de resistencia GRM, se produjo un aumento significativo de 4.734 pg/mL a 5.050 pg/mL de IL-10 (p=0,002). En cuanto a la TFG, no se encontraron resultados significativos. En cuanto a la composición corporal, hubo diferencias significativas en la TFG debido al aumento de la masa magra en brazos de 6.441g a 7.014g (p=0,04), piernas de 16.379g a 17.281g (p=0,02) y cuerpo entero de 45.640g a 47.343g (p=0,01). En el GRM hubo una disminución significativa del porcentaje de grasa en los brazos de 23.160% a 20.750% (p = 0,04). Para la evaluación de la calidad de vida se utilizó el cuestionario WHOQOL-HIV-Bref, donde se encontró una mejoría significativa en todos los dominios, excepto en el dominio nivel de independencia. Conclusión: Concluimos que el entrenamiento de resistencia muscular es eficaz para aumentar la IL-10 y disminuir el porcentaje de grasa en los brazos, mientras que el entrenamiento de fuerza aumenta la masa magra total. Nivel de Evidencia I; Ensayo clínico aleatorizado. Introducción VIHSIDA VIH SIDA pública embargo TARV (TARV Objetivos Métodos aleatorizado 1 (TFG GRM. . (GRM) IL2, IL2 IL 2, 2 IL-2 IL4, IL4 4, 4 IL-4 IL6, IL6 6, 6 IL-6 IL10 10 IL-1 TNFα TNF α sesión 3 semanas Resultados 4734 734 4.73 pgmL pg mL 5050 5 050 5.05 p=0,002. p0002 p p=0,002 0 002 (p=0,002) significativos 6441g g 441g 7014g 7 014g p=0,04, p004 p=0,04 , 04 (p=0,04) 16379g 16 379g 17281g 17 281g p=0,02 p002 02 (p=0,02 45640g 45 640g 47343g 47 343g p=0,01. p001 p=0,01 01 (p=0,01) 23160 23 160 23.160 20750 20 750 20.750 0,04. 004 0,04 0,04) WHOQOLHIVBref, WHOQOLHIVBref WHOQOL HIV Bref, Bref WHOQOL-HIV-Bref dominios independencia Conclusión total I (GRM IL- IL1 473 73 4.7 505 05 5.0 p000 p=0,00 00 (p=0,002 p00 p=0,0 (p=0,04 (p=0,0 (p=0,01 2316 23.16 2075 75 20.75 0,0 4. 50 5. (p=0,00 p0 p=0, (p=0, 231 23.1 207 20.7 0, p=0 (p=0 23. 20. p= (p=

ABSTRACT Introduction: HIV/AIDS is considered one of the great cases of public health, but it is seen that patients who use antiretroviral therapy (ART) and practice strength training promote a promotion of their health. Objectives: Assess the impact of strength and resistance training on cytokines and body composition in people living with HIV/AIDS. Methods: Randomized clinical trial, the sample consisted of 12 patients, 7 from the Strength Group (GF) and 5 from Group 2, Muscular Resistance (MGR). We compared the levels of IL-2, IL-4, IL-6, IL-10 and TNF-α cytokines and body composition in the first and last sessions. The patients completed 36 strength and resistance training sessions over 12 weeks. Results: After 36 sessions of GRM resistance training, there was a significant increase from 4,734 pg/mL to 5,050 pg/mL of IL-10 (p=0.002). Regarding the GFR, no significant results were found. For body composition, there were significant differences in GFR due to the increase in lean mass of the arms from 6,441g to 7,014g (p=0.04), legs from 16,379g to 17,281g (p=0.02) and whole body of 45,640g to 47,343g (p=0.01). In G2 there was a significant decrease in the percentage of fat in the arms from 23,160% to 20,750% (p = 0.04). To assess quality of life, the WHOQOL-HIV-Bref questionnaire was used, where significant improvement was found in all domains, except for the level of independence domain. Conclusion: We conclude that muscular resistance training is effective in increasing IL-10 and decreasing the percentage of fat in the arms, whereas strength training increases lean mass in arms, legs, and the whole body. Level of Evidence I; Randomized Clinical Trial. Introduction HIVAIDS HIV AIDS health ART (ART Objectives Methods trial 1 GF (GF 2 MGR. MGR . (MGR) IL2, IL2 IL IL-2 IL4, IL4 4, 4 IL-4 IL6, IL6 6, 6 IL-6 IL10 10 IL-1 TNFα TNF α 3 weeks Results 4734 734 4,73 pgmL pg mL 5050 050 5,05 p=0.002. p0002 p p=0.002 0 002 (p=0.002) 6441g g 441g 7014g 014g p=0.04, p004 p=0.04 , 04 (p=0.04) 16379g 16 379g 17281g 17 281g p=0.02 p002 02 (p=0.02 45640g 45 640g 47343g 47 343g p=0.01. p001 p=0.01 01 (p=0.01) G 23160 23 160 23,160 20750 20 750 20,750 0.04. 004 0.04 0.04) life WHOQOLHIVBref WHOQOL Bref used domains domain Conclusion I Trial (MGR IL- IL1 473 73 4,7 505 05 5,0 p000 p=0.00 00 (p=0.002 p00 p=0.0 (p=0.04 (p=0.0 (p=0.01 2316 23,16 2075 75 20,75 0.0 50 5, (p=0.00 p0 p=0. (p=0. 231 23,1 207 20,7 0. p=0 (p=0 23, 20, p= (p=

RESUMO Introdução: O HIV/AIDS é considerado um dos grandes casos de saúde pública, porém verifica-se que pacientes que fazem uso de terapia antirretroviral (TARV) e praticam treinamento de força provocam uma promoção de sua saúde. Objetivos: Avaliar o impacto do treinamento de força sobre a resistência nas citocinas e a composição corporal de pessoas vivendo com HIV/AIDS. Métodos: Ensaio clínico randomizado, a amostra foi composta por 12 pacientes, sendo sete do Grupo Força (TFG) e cinco do Grupo Resistência Muscular (GRM). Comparou-se os níveis das citocinas IL-2, IL-4, IL-6, IL-10 e TNF-α e a composição corporal na primeira e na última sessão. Os pacientes completaram 36 sessões de treinamento de força e resistência ao longo de 12 semanas. Resultados: Após 36 sessões de treinamento resistido GRM, houve um aumento significativo de 4.734 pg/mL para 5.050 pg/mL de IL-10 (p=0,002). Em relação à TFG, não foram encontrados resultados significativos. Para composição corporal, houve diferenças significativas na TFG devido ao aumento da massa magra dos braços de 6.441g para 7.014g (p=0,04), pernas de 16.379g para 17.281g (p=0,02) e corpo inteiro de 45.640g para 47.343g (p=0,01). No GRM houve diminuição significativa do percentual de gordura nos braços de 23.160% para 20.750% (p = 0,04). Para avaliação da qualidade de vida foi utilizado o questionário WHOQOL-HIV-Bref, onde foi encontrada uma melhora significativa em todos os domínios, exceto no domínio nível de independência. Conclusão: Conclui-se que o treinamento de resistência muscular é eficaz em aumentar a IL-10 e diminuir o percentual de gordura nos braços, enquanto o treinamento de força aumenta a massa magra geral. Nível de Evidência I; Ensaio Clínico Randomizado. Introdução HIVAIDS HIV AIDS pública verificase verifica se TARV (TARV Objetivos Métodos randomizado 1 (TFG GRM. . (GRM) Comparouse Comparou IL2, IL2 IL 2, 2 IL-2 IL4, IL4 4, 4 IL-4 IL6, IL6 6, 6 IL-6 IL10 10 IL-1 TNFα TNF α sessão 3 semanas Resultados 4734 734 4.73 pgmL pg mL 5050 5 050 5.05 p=0,002. p0002 p p=0,002 0 002 (p=0,002) significativos 6441g g 441g 7014g 7 014g p=0,04, p004 p=0,04 , 04 (p=0,04) 16379g 16 379g 17281g 17 281g p=0,02 p002 02 (p=0,02 45640g 45 640g 47343g 47 343g p=0,01. p001 p=0,01 01 (p=0,01) 23160 23 160 23.160 20750 20 750 20.750 0,04. 004 0,04 0,04) WHOQOLHIVBref, WHOQOLHIVBref WHOQOL Bref, Bref WHOQOL-HIV-Bref domínios independência Conclusão Concluise Conclui geral I Randomizado (GRM IL- IL1 473 73 4.7 505 05 5.0 p000 p=0,00 00 (p=0,002 p00 p=0,0 (p=0,04 (p=0,0 (p=0,01 2316 23.16 2075 75 20.75 0,0 4. 50 5. (p=0,00 p0 p=0, (p=0, 231 23.1 207 20.7 0, p=0 (p=0 23. 20. p= (p=

Abstract The present study sought to evaluate the antimicrobial and anti-adherent potential of Eucalyptus radiata essential oil against food-borne strains of Escherichia coli. The study was performed using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). In addition, the disk diffusion technique was used to evaluate the association of Eucalyptus radiata essential oil with synthetic antimicrobials. The Minimum Inhibitory Adherence Concentration (MIC) was also performed. The results revealed that E. radiata showed antimicrobial activity against the E. coli strains tested, with MIC values ranging from 500 μg/mL to 1000 μg/mL and MBC values ranging from 500 μg/mL to 1,024 μg/mL. As for the associations, it was observed that E. radiata oil exhibited a synergistic effect for some antibiotics, especially Ceftriaxone, with greater interference from the essential oil. Furthermore, it was effective in inhibiting the adherence of bacterial strains of E. coli, showing a more significant antibiofilm effect than the antibacterial agent 0.12% chlorhexidine digluconate. In summary, the essential oil of E. radiata showed antimicrobial potential against strains of E. coli of food origin, and can therefore, through in-depth studies, be used alone or in association with synthetic antimicrobials to combat infections caused by this pathogen. antiadherent anti adherent foodborne borne (MIC MBC. . (MBC) addition E tested 50 μgmL μg mL 100 1024 1 024 1,02 associations antibiotics Ceftriaxone Furthermore 012 0 12 0.12 digluconate summary origin therefore indepth depth studies pathogen (MBC 5 10 102 02 1,0 01 0.1 1, 0.

Resumo O presente estudo buscou avaliar o potencial antimicrobiano e antiaderente do óleo essencial de Eucalipto radiata frente às cepas de veiculação alimentar de Escherichia coli. O estudo foi realizado usando a Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM). Além disso, a técnica de difusão em disco foi usada para avaliar a associação do óleo essencial de Eucalipto radiata com antimicrobianos sintéticos. A Concentração Inibitória Mínima de Aderência (CIMA) também foi realizada. Os resultados revelaram que E. radiata apresentou atividade antimicrobiana contra as cepas de E. coli testadas, com valores de CIM variando entre 500 μg/mL a 1000 μg/mL e valores de CBM variando entre 500 μg/mL a 1,024 μg/mL. Quanto às associações, observou-se que o óleo de E. radiata exibiu efeito sinérgico para alguns antibióticos, sobretudo a Ceftriaxona, apresentando maior interferência do óleo essencial. Além disso, foi eficaz na inibição a aderência das cepas bacterianas de E. coli, mostrando um efeito antibiofilme mais significativo que o agente antibacteriano digluconato de clorexidina a 0.12%. Em resumo, o óleo essencial de E. radiata apresentou potencial antimicrobiano contra cepas de E. coli de origem alimentar, podendo assim, mediante estudos aprofundados, ser utilizado isoladamente ou em associação com antimicrobianos sintéticos para combater infecções causadas por esse patógeno. (CIM CBM. . (CBM) disso CIMA (CIMA realizada E testadas 50 μgmL μg mL 100 1024 1 024 1,02 associações observouse observou se antibióticos Ceftriaxona 012 0 12 0.12% resumo assim aprofundados patógeno (CBM 5 10 102 02 1,0 01 0.12 1, 0.1 0.

ABSTRACT The use growth regulators from the cytokinins and auxins group has been shown to increase the productivity of certain crops, as they may be involved in the expression of plant sex, although little is known about the action of auxin IBA and cytokinin CPPU on the fruiting of squash, as well as the interaction between both, which makes this field of study promising. Thus, the objective of this study was to induce the fruiting in Atlas squash, by means of different forms of application and proportions of cytokinin and auxin and to evaluate their effects on the physical-chemical characteristics of the fruits. The treatments were composed of six proportions associated with growth regulators and of two forms of application. The experimental design was a randomized block design with four replicates. At 72 days after transplanting, growth and fruit quality analyses were performed. The behavior of the hormones is modulated by their concentration and place of application, and the effect may be beneficial or not. The proportions 50/50% via leaf and 75/25% via ovary were the ones that increased the growth and parameters of fruit quality. The application of CPPU via leaf at higher concentration in proportion decreases quality characteristics such as vitamin C, carotenoids and starch. crops sex squash both promising Thus physicalchemical physical chemical fruits replicates 7 transplanting performed not 5050 50 50/50 7525 75 25 75/25 C starch 505 5 50/5 752 2 75/2 50/ 75/

RESUMO O uso de reguladores de crescimento do grupo das citocininas e auxinas tem demonstrado aumentar a produtividade de algumas culturas, pois podem estar envolvidos na expressão do sexo da planta, embora pouco se saiba sobre a ação da auxina IBA e da citocinina CPPU na frutificação da abóbora, bem como a interação entre ambas, o que torna este campo de estudo promissor. Assim, o objetivo deste estudo foi induzir a frutificação em abóbora Atlas, por meio de diferentes formas de aplicação e proporções de citocinina e auxina e avaliar seus efeitos nas características físico-químicas dos frutos. Os tratamentos foram compostos por seis proporções associadas a reguladores de crescimento e duas formas de aplicação. O delineamento experimental foi em blocos casualizados com quatro repetições. Aos 72 dias após o transplante, foram realizadas análises de crescimento e qualidade dos frutos. O comportamento dos hormônios é modulado por sua concentração e local de aplicação, podendo o efeito ser benéfico ou não. As proporções 50/50% via folha e 75/25% via ovário foram as que aumentaram o crescimento e os parâmetros de qualidade dos frutos. A aplicação de CPPU via foliar em maior concentração em proporção diminui características de qualidade como vitamina C, carotenoides e amido. culturas planta ambas promissor Assim Atlas físicoquímicas físico químicas frutos repetições 7 transplante não 5050 50 50/50 7525 75 25 75/25 C amido 505 5 50/5 752 2 75/2 50/ 75/

A solution blow spinning (SBS) process was utilized to create nanofibers of TiO2/C and TiO2/C/N with potential applications in photocatalysis and dye adsorption. Polyvinylpyrrolidone was used as a carbon and nitrogen source. Their content in the TiO2 structure decreased as the temperature increased from 350 to 550 °C. Physical-chemical, morphological, and structural features were investigated. Adsorption and photodegradation experiments revealed that the material calcined at 400 °C had higher dye removal (98%) under dark settings, whereas the greater photodegradation efficiency under visible and UV light was 56% and 98%, respectively, for the sample calcined at 550 °C. The results suggest that the SBS has the potential to develop an efficient adsorbent and photocatalyst for use in treatments of contaminated water. (SBS TiO2C TiOC C TiO TiO2CN TiOCN N adsorption source 35 55 Physicalchemical, Physicalchemical Physical chemical, chemical Physical-chemical morphological investigated 40 98% 98 (98% settings 56 respectively water CN 3 5 4 9 (98 (9 (

ABSTRACT: In the last decade, there has been a significant increase in the demand for small rodents such as hamsters, guinea pigs, rats, and mice to be kept as pets. Consequently, the veterinary care provided to these animals has also increased. The aim of this study was to describe gross, histopathological and immunohistochemical findings of 26 spontaneous neoplasms diagnosed in 25 pet rodents in Northeastern Brazil. A retrospective study was carried out from 2014 to 2022 in two veterinary diagnostic laboratories to identify cases of tumoriform lesions in these species. Hamsters represented the most frequent species in this study (60%, 15/25), followed by rats (28%, 7/25), guinea pigs (8%, 2/25), and mice (4%, 1/25), with a mean age of 17.63 months. The anatomical regions of the face/head and thoracic region were the most affected. The most affected tissues were the skin/subcutaneous/mucosal (65%, 15/26) and mammary gland (23%, 6/26). Eighteen different types of neoplasms were diagnosed, and T-cell lymphomas and mammary adenocarcinomas were the most diagnosed tumors, each corresponding to 12% (3/26) of the cases. Leiomyosarcoma, myxosarcoma and mammary adenocarcinoma metastases were also noted. Immunohistochemistry was essential for the diagnosis of certain malignant mesenchymal and round-cell neoplasms. Pet rodent neoplasms in Northeastern Brazil are common, especially in hamsters, and immunohistochemistry can be a useful tool for the definitive diagnosis of these tumors. ABSTRACT decade hamsters pets Consequently increased gross 2 201 202 60%, 60 (60% 15/25, 1525 15/25 , 15 15/25) 28%, 28 (28% 7/25, 725 7/25 7 7/25) 8%, 8 (8% 2/25, 225 2/25 2/25) 4%, 4 (4% 1/25, 125 1/25 1 1/25) 1763 17 63 17.6 months facehead face head skinsubcutaneousmucosal skin subcutaneous mucosal 65%, 65 (65% 15/26 1526 23%, 23 (23% 6/26. 626 6/26 . 6 6/26) Tcell T cell tumors 12 3/26 326 3 (3/26 Leiomyosarcoma noted roundcell round common 20 60% (60 152 15/2 28% (28 72 7/2 8% (8 22 2/2 4% (4 1/2 176 17. 65% (65 23% (23 62 6/2 3/2 32 (3/2 (6 15/ (2 7/ ( 2/ 1/ 6/ 3/ (3/ (3

Gamma radiation is an effective method for microbial decontamination of plant materials. However, this treatment can induce changes in the chemical structure of plant constituents. Stem barks of Luehea ochrophylla were exposed to different doses of gamma radiation to evaluate decontamination efficiency and changes in chemical composition of plant material including phenolic fraction. The major microbial contaminants of non-irradiated samples were isolated and identified as the fungal species Eurotium chevalieri L. Mangin and Lecythophora decumbens. The dose of 5.0 kGy was effective to achieve total decontamination of the stem barks of L. ochrophylla. The formation of free radicals was verified in the plant material using electron paramagnetic resonance spectroscopy, and was supposed to be related to the trans-aconitic acid, a plant constituent. It was the only secondary metabolite to have its concentration significantly altered with radiation in phenolic fraction, as observed by liquid chromatography with diode array detector coupled to mass spectroscopy (LC-DAD-MS). The trans-aconitic acid was isolated and exposed to gamma radiation in aqueous medium. Its concentration decreased after exposition to a dose of 3.0 kGy, corroborating the supposition of its degradation. Citric acid was the main radiolytic product formed by irradiation of trans-aconitic acid in the presence of water. materials However constituents fraction nonirradiated non irradiated L decumbens 50 5 0 5. transaconitic trans aconitic constituent LCDADMS. LCDADMS LC DAD MS . (LC-DAD-MS) medium 30 3 3. degradation water (LC-DAD-MS

The industry's principal objective is to comprehend the optimal application of each metal alloy in terms of corrosion resistance. A key focus lies in exploring alternative approaches to address this corrosive process in a manner that is both sustainable and economically feasible. The major strategy to mitigate this contest involves the utilization of inhibitors. However, it is imperative to note that certain inhibitors pose environmental risks. This study explores the effect of the addition of protic ionic liquids (PILs) on the corrosion of carbon steel (A36) in a saline solution (3.5 wt% NaCl), considering variations in pH and temperature. It focuses on understanding the effects of pH and temperature on the PILs' ability to protect the steel surface. Notably, changes in pH do not affect the protective capacity of the inhibitors. Efficiency values exceeding 72% were achieved under both acidic conditions tested. The study reveals two scenarios: At 40 °C, the PILs effectively protect the steel, with an efficiency of approximately 74% at concentrations of 500 and 1000 ppm. However, at 60 °C, efficiency decreases notably, reaching a maximum of 51% at a concentration of 500 ppm. Afterward, to evaluate the protective effectiveness of these compounds, gravimetric and electrochemical impedance spectroscopy (EIS) serve as the main methods, accompanied by optical and atomic force microscopy (AFM) for evaluating the surface. In electrochemical tests, PILs 1 and 2 exhibit superior efficiency, with film formation, while PILs 3 to 6 demonstrate comparatively lower values. industrys industry s resistance feasible However risks (PILs A36 (A36 3.5 35 5 (3. wt NaCl, NaCl , NaCl) surface Notably 72 tested scenarios 4 C °C 74 50 100 ppm notably 51 Afterward compounds EIS (EIS methods AFM (AFM tests formation A3 (A3 3. (3 7 10 (A (