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Bacillus-based probiotics on broiler chicken performance under coccidiosis and Clostridium perfringens challenge
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Berto, Rafaela Rohloff Junior, Nilton dos Santos Andrade, Thiago Kaufmann, Cristine Sartor, Heloisa Costa, Ana Paula Guimarães Cruz Comin, Gabriel Natã Toniazzo, Gabrielli de Queiroz Teixeira, Vinicius Naranjo, Victor Daniel Eyng, Cinthia Nunes, Ricardo Vianna

South African Journal of Animal Science

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2024, Volume 54 Nº 4 Páginas 509 - 523

Resumo: En | Texto: En | PDF: En

The study analyzed the impacts of two probiotics (Ecobiol® and GutCare®) on growth performance, carcass and cut yields, intestinal morphometry and lesion score, biochemical parameters, and short-chain fatty acid concentration in chickens using a challenge model combining coccidiosis vaccine and Clostridium perfringens. A total of 880 one-day-old male broilers (Cobb 500) were randomly assigned to four treatments with 10 replicates and 22 birds per experimental unit (EU). The treatments consisted of: PC: positive control with the inclusion of 80 g ton-1 of Enramax® (8% enramycin) until 35 d of age; NC: negative control without the inclusion of growth-promoting probiotic; BA: NC with the inclusion of 1000 g ton-1 of Ecobiol® (Bacillus amyloliquefaciens - CECT 5940); BS: NC with the inclusion of 500 g ton-1 of GutCare® (Bacillus subtilis - DSM 32315). Diets used maize-soybean meal in a three-phase plan. Broiler chickens on the BA and BS diets had higher feed consumption, body weight gain, and improved feed conversion efficiency at 28 and 42 d when compared to the birds in the negative control. Broiler chickens fed PC, BA, and BS diets had a higher villus height and absorption area in the jejunum at 28 d, compared to the birds in the negative control. There was more butyric acid production by the intestinal microbiota at 28 d of age in broilers on the BA diet. Supplementing with 1000 g/ton of BA and 500 g/ton of BS effectively substituted the 8% enramycin antibiotic, enhancing broiler growth during an induced intestinal challenge.

https://doi.org/10.4314/sajas.v54i4.04

Impact of harvest delay on the physiological and sanitary quality of Sorghum sp. seeds
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Martins, Antonio Guimarães

; Zuffo, Alan Mario

; Barrozo, Leandra Matos

; Mezzomo, Ricardo

; Silva, Francisco Charles dos Santos

; Steiner, Fabio

; Santos, Raianara Andrade dos

; Alves, Charline Zaratin

Revista Ciência Agronômica

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2024, Volume 55 elocation e20238802

Resumo: En | Texto: En | PDF: En

ABSTRACT Harvest delay can alter the physiological and sanitary quality of sorghum seeds due to the longer exposure time of seeds to adverse environmental conditions in the field. In this study, we investigated the impact of delayed Sorghum sp. harvest on the physiological and sanitary quality of seeds in four hybrids grown in the tropical soil of the Brazilian Cerrado region. Plants from four Sorghum sp. hybrids (IPA SUDAN 4202, BRS 658, BRS 810, and BRS 373) were grown and submitted to four seed harvest periods (0, 7, 14, and 21 days after stage 9 - seed physiological maturity). The treatments were arranged in a completely randomized block design in a 4 × 4 factorial, with four replicates. At each harvest period, the thousand-seed weight, water content, first count of the germination test, germination, seedling emergence, emergence speed index, seedling dry matter, accelerated aging, electrical conductivity, and sanitary quality of seeds were determined. Seeds of higher physiological and sanitary quality of the IPA SUDAN 4202 and BRS 658 sorghum hybrids can be obtained when the harvest is carried out up to 7 days after the physiological maturity stage. However, the delay in seed harvesting at 14 and 21 days after physiological maturity results in a higher incidence of Colletotrichum sp., Curvularia sp., Rhizoctonia sp., and Alternaria sp. on the seeds of these hybrids. Delaying the harvest of sorghum seeds from 7 days after the physiological maturity stage compromises seed vigor and viability and increases the incidence of pathogens.

https://doi.org/10.5935/1806-6690.20230068

Mast cells and factor XIIIa+ dendrocytes in actinic cheilitis and lip squamous cell carcinoma
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Flores, Isadora Luana

; de Arruda, José Alcides Almeida

; Abrantes, Thamiris de Castro

; Gamba, Thiago de Oliveira

; Abrahão, Aline Correa

; Anbinder, Ana Lia

; Ribeiro, Jaqueline Lemes

; Vasconcelos, Ana Carolina Uchoa

; Andrade, Bruno Augusto Benevenuto de

; Aguiar, Maria Cassia Ferreira de

; Gomes, Ana Paula Neutzling

; Abreu, Lucas Guimarães

; Mesquita, Ricardo Alves

Brazilian Oral Research

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2024, Volume 38 elocation e113

Resumo: En | Texto: En | PDF: En

Abstract There is an interaction between dendrocytes and mast cells in the skin. However, in elastosis-related diseases such as actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC), this interaction remains unknown. We investigated the presence of intact and degranulated mast cells in AC and LLSCC. Associations of mast cells with factor XIIIa+ dendrocytes and inflammatory infiltrate were assessed. Forty cases of AC (20 with low-grade and 20 with high-grade epithelial dysplasia), 50 cases of LLSCC, and 10 cases of normal oral mucosa were evaluated. Toluidine blue staining was performed to identify mast cells, and mast cell densities were calculated in the inflammatory infiltrate. Factor XIIIa+ dendrocytes were immunohistochemically quantified. The highest ratio of intact/degranulated mast cells density was detected in LLSCC (5.9 cells/mm2), followed by AC with high-grade epithelial dysplasia (4.8 cells/mm2). Statistically significant differences were found in the density of intact mast cells compared to degranulated mast cells in AC with low-grade epithelial dysplasia (p<0.001), AC with high-grade epithelial dysplasia (p=0.005), and LLSCC (p<0.001). A positive correlation between degranulated mast cells and total inflammatory infiltrate (p=0.03) was observed in the LLSCC group. The expression of factor XIIIa+ dendrocytes was highest in AC with low-grade epithelial dysplasia (16.5 cells/mm2). The link between mast cell density, factor XIIIa+ dendrocytes, and inflammatory infiltrate indicates a potential crosstalk in lip carcinogenesis.

https://doi.org/10.1590/1807-3107bor-2024.vol38.0113

Dapagliflozin in patients with critical illness: rationale and design of the DEFENDER study
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Tavares, Caio de Assis Moura

; Azevedo, Luciano César Pontes de

; Rea-Neto, Álvaro

; Campos, Niklas Söderberg

; Amendola, Cristina Prata

; Bergo, Ricardo Reinaldo

; Kozesinski-Nakatani, Amanda Christina

; David-João, Paula Geraldes

; Westphal, Glauco Adrieno

; Guimarães Júnior, Mário Roberto Rezende

; Lobo, Suzana Margareth Ajeje

; Tavares, Marcos Soares

; Dracoulakis, Marianna Deway Andrade

; Souza, Guilherme Martins de

; Almeida, Guacyra Margarita Batista de

; Gebara, Otavio Celso Eluf

; Tomba, Pablo Oscar

; Albuquerque, Camila Santos N

; Silva, Mariana Castaldi Ramalho

; Pereira, Adriano José

; Damiani, Lucas Petri

; Corrêa, Thiago Domingos

; Serpa-Neto, Ary

; Berwanger, Otavio

; Zampieri, Fernando Godinho

Critical Care Science

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Set 2023, Volume 35 Nº 3 Páginas 256 - 265

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Background: Critical illness is a major ongoing health care burden worldwide and is associated with high mortality rates. Sodium-glucose cotransporter-2 inhibitors have consistently shown benefits in cardiovascular and renal outcomes. The effects of sodium-glucose cotransporter-2 inhibitors in acute illness have not been properly investigated. Methods: DEFENDER is an investigator-initiated, multicenter, randomized, open-label trial designed to evaluate the efficacy and safety of dapagliflozin in 500 adult participants with acute organ dysfunction who are hospitalized in the intensive care unit. Eligible participants will be randomized 1:1 to receive dapagliflozin 10mg plus standard of care for up to 14 days or standard of care alone. The primary outcome is a hierarchical composite of hospital mortality, initiation of kidney replacement therapy, and intensive care unit length of stay, up to 28 days. Safety will be strictly monitored throughout the study. Conclusion: DEFENDER is the first study designed to investigate the use of a sodium-glucose cotransporter-2 inhibitor in general intensive care unit patients with acute organ dysfunction. It will provide relevant information on the use of drugs of this promising class in critically ill patients. ClinicalTrials.gov registry: NCT05558098

RESUMO Antecedentes: A doença crítica é um importante ônus permanente da assistência médica em todo o mundo e está associada a altas taxas de mortalidade. Os inibidores do cotransportador de sódio-glicose do tipo 2 têm demonstrado consistentemente benefícios nos desfechos cardiovasculares e renais. Os efeitos dos inibidores do cotransportador de sódio-glicose do tipo 2 em doenças agudas ainda não foram devidamente investigados. Métodos: O DEFENDER é um estudo de iniciativa do investigador, multicêntrico, randomizado, aberto, desenhado para avaliar a eficácia e a segurança da dapagliflozina em 500 participantes adultos com disfunção orgânica aguda hospitalizados na unidade de terapia intensiva. Os participantes aptos serão randomizados 1:1 para receber 10mg de dapagliflozina e o tratamento padrão por até 14 dias ou apenas o tratamento padrão. O desfecho primário é um composto hierárquico de mortalidade hospitalar, início de terapia renal substitutiva e tempo de internação na unidade de terapia intensiva, até 28 dias. O monitoramento da segurança será rigoroso durante todo o estudo. Conclusão: O DEFENDER é o primeiro estudo desenvolvido para investigar o uso de um inibidor do cotransportador de sódio-glicose do tipo 2 em pacientes de unidade de terapia intensiva geral com disfunção orgânica aguda. O estudo fornecerá informações relevantes sobre o uso de medicamentos dessa classe promissora em pacientes críticos. Registro ClincalTrials.gov: NCT05558098

https://doi.org/10.5935/2965-2774.20230129-en

Contribution of public oral pathology services to the diagnosis of oral and oropharyngeal cancer in Brazil
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LOUREDO, Brendo Vinicius Rodrigues

; CURADO, Maria Paula

; PENAFORT, Paulo Victor Mendes

; DE ARRUDA, José Alcides Almeida

; ABREU, Lucas Guimarães

; MESQUITA, Ricardo Alves

; PINTO-JÚNIOR, Décio dos Santos

; ABRAHÃO, Aline Corrêa

; ANDRADE, Bruno Augusto Benevenuto de

; AGOSTINI, Michelle

; MORAES, Renata Mendonça

; ANBINDER, Ana Lia

; DOURADO, Pedro Henrique Silva

; SANTOS, Teresa Cristina Ribeiro Bartholomeu dos

; PIRES, Fábio Ramoa

; BORDIGNON, Natalia Cristina Trentin

; GONDAK, Rogério Oliveira

; DE OLIVEIRA, Marcia Gaiger

; CARRARD, Vinicius Coelho

; MARTINS, Manoela Domingues

; SOUSA-NETO, Sebastião Silvério

; ARANTES, Diego Antônio Costa

; MENDONÇA, Elismauro Francisco

; CIESLAK-SANCHES, Silvia Roberta

; ANTUNES, Daniella Moraes

; AMARAL-SILVA, Gleyson Kleber do

; MANIERI, Patricia Rubia

; RAMALHO, Luciana Maria Pedreira

; DOS SANTOS, Jean Nunes

; LEONEL, Augusto César Leal da Silva

; PEREZ, Danyel Elias da Cruz

; VERHEUL, Hannah Carmem Carlos Ribeiro Silva

; BARROSO, Keila Martha Amorim

; RODRIGUES, Flávia Luiza Santos

; GONZAGA, Amanda Katarinny Goes

; FERNANDES, Romana Renery

; DE SOUZA, Lélia Batista

; SOUZA, Lucas Lacerda de

; PONTES, Flávia Sirotheau Corrêa

; PONTES, Hélder Antônio Rebelo

; SILVA, Caroline Alfaia

; CÂMARA, Jeconias

; LIBÓRIO-KIMURA, Tatiana Nayara

; SANTOS-SILVA, Alan Roger

; LOPES, Márcio Ajudarte

; ALMEIDA, Oslei Paes de

; ROMAÑACH, Mário José

; VARGAS, Pablo Agustin

Brazilian Oral Research

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2023, Volume 37 elocation e126

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to evaluate the contribution of oral and maxillofacial pathology laboratories (OMPLs) in Brazilian public universities to the diagnosis of lip, oral cavity, and oropharyngeal squamous cell carcinoma (SCC). A cross-sectional study was performed using biopsy records from a consortium of sixteen public OMPLs from all regions of Brazil (North, Northeast, Central-West, Southeast, and South). Clinical and demographic data of patients diagnosed with lip, oral cavity, and oropharyngeal SCC between 2010 and 2019 were collected from the patients’ histopathological records. Of the 120,010 oral and maxillofacial biopsies (2010-2019), 6.9% (8,321 cases) were diagnosed as lip (0.8%, 951 cases), oral cavity (4.9%, 5,971 cases), and oropharyngeal (1.2%, 1,399 cases) SCCs. Most cases were from Brazil’s Southeast (64.5%), where six of the OMPLs analyzed are located. The predominant profile of patients with lip and oral cavity SCC was Caucasian men, with a mean age over 60 years, low schooling level, and a previous history of heavy tobacco consumption. In the oropharyngeal group, the majority were non-Caucasian men, with a mean age under 60 years, had a low education level, and were former/current tobacco and alcohol users. According to data from the Brazilian National Cancer Institute, approximately 9.9% of the total lip, oral cavity, and oropharyngeal SCCs reported over the last decade in Brazil may have been diagnosed at the OMPLs included in the current study. Therefore, this data confirms the contribution of public OMPLs with respect to the important diagnostic support they provide to the oral healthcare services extended by the Brazilian Public Health System.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0126

STRENGTH TRAINING IN PATIENTS WITH FIBROMYALGIA: A FEASIBILITY STUDY
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Andrade, Alexandro

; Steffens, Ricardo de Azevedo Klumb

; Grisard, Felippe

; Liz, Carla Maria de

; Brandt, Ricardo

; Coimbra, Danilo Reis

; Bevilacqua, Guilherme Guimarães

Revista Brasileira de Medicina do Esporte

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2023, Volume 29 elocation e176543

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Introdução: A síndrome da fibromialgia (FM) é caracterizada pela presença de dor difusa com duração de mais de três meses e, frequentemente, é associada a distúrbios do sono. Estudos investigaram o efeito do treinamento de força (ST) sobre a dor e a qualidade do sono dos pacientes com FM, mas ainda existem diversas perspectivas quanto aos efeitos da intervenção nessa população. Objetivos: O objetivo do estudo foi examinar os efeitos do treinamento de força (ST) sobre a dor e a qualidade do sono de pacientes com FM. Métodos: Quarenta e oito mulheres com FM participaram do estudo entre agosto e outubro de 2012. Seis (55 ± 6,5 anos) realizaram treinamento de força, conduzido na Universidade Estadual de Santa Catarina, e oito (47 ± 9 anos) constituíram o grupo controle. Foram empregados o Questionário Sociodemográfico e Clínico, a escala visual analógica (EVA) para dor e o Índice de Qualidade do Sono de Pittsburgh. Os dados foram coletados antes da primeira sessão e depois da intervenção de oito semanas e foram analisados por meio de estatística descritiva e testes inferenciais. Resultados: A intervenção de 8 semanas com ST diminuiu a dor (p < 0,05) e reduziu significativamente as disfunções diurnas do sono (p <0,05), demonstrando que o programa proposto contribui para melhorar a qualidade de vida dos pacientes. Conclusão: O ST é um tratamento viável para pacientes com fibromialgia. Nível de evidência II; Estudo terapêutico.

ABSTRACT Introduction: Fibromyalgia syndrome (FM) is characterized by the presence of diffuse pain lasting for more than three months and is often associated with sleep disorders. Studies have investigated the effect of strength training (ST) on pain and sleep quality in FM patients, but there continue to be diverse perspectives on the effects of this intervention in this population. Objective: The aim of the study was to examine the effects of strength training (ST) on pain and sleep quality in FM patients. Methods: Forty-eight women with FM participated in the study between August and October, 2012. Six (55±6.5 years) performed ST, conducted at Santa Catarina State University, and eight (47±9 years) comprised the control group. The Socio-Demographic and Clinical Questionnaire, the visual analog scale (VAS) for pain, and the Pittsburgh Sleep Quality Index were used. Data were collected before the first session and after the eight-week intervention and were analyzed using descriptive statistics and inferential tests. Results: The eight- week ST intervention decreased pain (p< 0.05) and significantly diminished the daytime sleep dysfunctions (p< 0.05), demonstrating that the proposed program contributes to improving patient quality of life. Conclusion: ST is a feasible treatment for patients with fibromyalgia. Level of evidence II; Therapeutic study.

RESUMEN Introducción: El síndrome de fibromialgia (FM) se caracteriza por la presencia de dolor difuso de más de tres meses de duración y suele asociarse a trastornos del sueño. Los estudios han investigado el efecto del entrenamiento de fuerza (ST) sobre el dolor y la calidad del sueño en pacientes con FM, pero todavía existen diversas perspectivas respecto a los efectos de esta intervención en esta población. Objetivos: El objetivo del estudio fue examinar los efectos del entrenamiento de fuerza sobre el dolor y la calidad del sueño de los pacientes con FM. Métodos: Cuarenta y ocho mujeres con FM participaron en el estudio entre agosto y octubre de 2012. Seis (55 ± 6,5 años) se sometieron a un entrenamiento de fuerza realizado en la Universidad del Estado de Santa Catarina, y ocho (47 ± 9 años) constituyeron el grupo de control. Se utilizó el Cuestionario sociodemográfico y Clínico, la escala visual analógica (EVA) para el dolor y el Índice de Calidad del Sueño de Pittsburgh. Los datos fueron recopilados antes de la primera sesión y después de la intervención de ocho semanas y se analizaron mediante estadísticas descriptivas y pruebas de inferencia. Resultados: La intervención de ocho semanas con ST disminuyó el dolor (p <0,05) y redujo significativamente los trastornos del sueño durante el día (p <0,05), lo que demuestra que el programa propuesto contribuye a mejorar la calidad de vida de los pacientes. Conclusión: El entrenamiento de fuerza es un tratamiento viable para pacientes con fibromialgia. Nivel de evidencia II; Estudios terapéuticos.

https://doi.org/10.1590/1517-869220232901176543

Clinical features of oral lichen planus and oral lichenoid lesions: an oral pathologist’s perspective
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Netto, Juliana de Noronha Santos

; Pires, Fábio Ramoa

; Costa, Karen Hurtado Andrade

; Fischer, Ricardo Guimarães

Brazilian Dental Journal

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Set 2022, Volume 33 Nº 3 Páginas 67 - 73

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Os achados clinico-patológicos que caracterizam de forma precisa o líquen plano oral (LPO) e as lesões liquenoides orais (LLO) ainda representam um desafio. O objetivo do presente estudo foi analisar, pela perspectiva do patologista oral, os achados clínicos do LPO e das LLO. Espécimes preenchendo os critérios histológicos para o diagnóstico de LPO e LLO, e também aqueles compatíveis com LPO (LPO-C), foram selecionados e as informações clínicas foram obtidas a partir das requisições laboratoriais. A amostra final foi composta de 221 casos, incluindo 119 LPO (53.8%), 65 LPO-C (29.4%) e 37 LLO (16.7%). As mulheres foram mais acometidas nos três grupos, mas o número de homens acometidos foi maior nas LLO. A média de idade foi menor no LPO (52.3 anos) em comparação com as LLO (57.9 anos) (p=0.020). O envolvimento da mucosa jugal e da língua foi mais frequente no LPO; o envolvimento gingival foi incomum nas LLO. O padrão reticular foi mais frequentemente encontrado no LPO, enquanto a associação dos padrões reticular e atrófico/erosivo/ulcerado foi mais comum no LPO-C e nas LLO (p=0.025). Em conclusão, o gênero e a média de idade dos pacientes, assim como a localização anatômica e a manifestação clínica das LLO são diferentes do LPO, e podem ajudar a melhor caracterizar este grupo de condições. Espécimes diagnosticados como LPO-C mostraram parâmetros clínicos mais próximos ao LPO.

Abstract The clinicopathological features that precisely characterize oral lichen planus (OLP) and oral lichenoid lesions (OLL) still represent a challenge. The aim of the present study was to analyze, from an oral pathologist perspective, the clinical features from OLP and OLL. Specimens fullfilling the histological criteria for OLP and OLL, and also compatible with OLP (OLP-C), were selected and clinical information was retrieved from the laboratory forms. The final sample was composed by 221 cases, including 119 OLP (53.8%), 65 OLP-C (29.4%) and 37 OLL (16.7%). Females were more affected in the three groups, but the number of males was higher in OLL. Mean age was lower in OLP (52.3 years) in comparison with OLL (57.9 years) (p=0.020). Buccal mucosa and tongue involvement was more frequent in OLP; gingival involvement was uncommon in OLL. The reticular pattern was more frequently found in OLP, while the association of reticular and atrophic/erosive/ulcerated patterns was more common in OLP-C and OLL (p=0.025). In conclusion, gender and mean age of the patients, and anatomical location and clinical manifestation of OLL are different from OLP, and could help to better characterize this group of conditions. Specimens diagnosed as OLP-C showed clinical parameters close to OLP.

https://doi.org/10.1590/0103-6440202204426

Altrenogest during early pregnancy modulates uterine glandular epithelium and endometrial growth factor expression at the time implantation in pigs
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Muro, Bruno Bracco Donatelli

; Leal, Diego Feitosa

; Carnevale, Rafaella Fernandes

; Torres, Mariana Andrade

; Mendonça, Maitê Vidal

; Nakasone, Denis Hideki

; Martinez, Cristian Hernando Garcia

; Ravagnani, Gisele Mouro

; Monteiro, Matheus Saliba

; Poor, André Pegoraro

; Martins, Simone Maria Massami Kitamura

; Viau, Priscila

; Oliveira, Cláudio Alvarenga de

; Castro, Raquel Vasconcelos Guimarães de

; Bessi, Brendon Willian

; Bressan, Fabiana Fernandes

; Pulz, Lidia Hildebrand

; Strefezzi, Ricardo Francisco

; Almond, Glen William

; Andrade, André Furugen Cesar de

Animal Reproduction

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2021, Volume 18 Nº 1 elocation e20200431

Resumo: En | Texto: En | PDF: En

Abstract This study evaluated the effects of supplying altrenogest from day 6-12 of pregnancy on the endometrial glandular epithelium, corpora lutea (CL) morphology, and endometrial and CL gene expression. A total of 12 crossbred females (Landrace × Large White) were used. The females were assigned to 4 treatments according to a random design with a 2 × 2 factorial arrangement, with two categories (sow or gilt) and two treatments (non-treated and treated with altrenogest). On day 6 of pregnancy, animals were allocated to one of the following groups: non-treated (NT, n = 6; 3 sows and 3 gilts), and (T, n = 6; 3 sows and 3 gilts) treated daily with 20 mg of altrenogest, from day 6-12 of pregnancy. All animals were euthanized on day 13 of pregnancy. All CLs were individually weighed, and their volume were determined. The endometrial glandular density (GD), mean glandular area (MGA), and vascular density (VD) were determined by histomorphometric and immunohistochemical analyses. Endometrium samples were collected and analyzed by qRT-PCR to evaluate the abundance of transcripts for VEGF and IGF-I. Females in the T group had higher MGA (P < 0.05) compared to the NT group. There was no effect of treatment on GD or VD for both experimental groups. Sows in the T group had augmented expression of IGF-I (P < 0.05). Progestagen had no detrimental effect on CL morphology. In conclusion, altrenogest improves the uterine environment during the peri-implantation period in pigs without compromising corpora lutea development.

https://doi.org/10.1590/1984-3143-ar2020-0431 127 downloads

Detection of SARS-CoV-2 through pool testing for COVID-19: an integrative review
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Costa, Murilo Soares

; Guimarães, Nathalia Sernizon

; Andrade, André Barbosa de

; Vaz-Tostes, Luiza Passini

; Oliveira, Rhuan Braga

; Simões, Madara da Silva

; Gelape, Gabriel de Oliveira

; Alves, Claudia Regina Lindgren

; Machado, Elaine Leandro

; Fonseca, Flávio Guimarães da

; Teixeira, Santuza Maria Ribeiro

; Sato, Hugo Itaru

; Takahashi, Ricardo Hiroshi Caldeira

; Tupinambás, Unaí

Revista da Sociedade Brasileira de Medicina Tropical

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2021, Volume 54 elocation e0276-2021

Resumo: En | Texto: En | PDF: En

Abstract INTRODUCTION: The pool testing technique optimizes the number of tests performed and reduces the delivery time of results, which is an interesting strategy for the health crisis caused by the COVID-19 pandemic. This integrative review investigated studies in which pool testing was carried out for epidemiological or screening purposes to analyze its clinical or cost effectiveness and assessed the applicability of this method in high-, middle-, and low-income countries. METHODS: This integrative review used primary studies published in the MEDLINE, EMBASE, Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), and Cochrane Library databases. RESULTS: A total of 435 studies were identified: 35.3% were carried out in Asia, 29.4% in Europe, 29.4% in North America, and 5.9% in Oceania. CONCLUSIONS: This review suggests that pool testing in the general population may be a useful surveillance strategy to detect new variants of SARS-CoV-2 and to evaluate the period of immunogenicity and global immunity from vaccines.

https://doi.org/10.1590/0037-8682-0276-2021 44 downloads

10.

Elevated prevalence of high-risk HPV in healthy oral mucosa of users and nonusers of drugs in Northeastern Brazil
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Ribeiro, Mariana Goveia Melo

; Marcolino, Larissa Doddi

; Ramos, Bruna Ribeiro de Andrade

; Trento, Cleverson Luciano

; Araujo, Karina Conceição Gomes Machado de

; Silva, José Rodrigo Santos

; Jain, Sona

; Gurgel, Ricardo Queiroz

; Silva, Márcia Guimarães

; Dolabella, Silvio Santana

Brazilian Oral Research

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2019, Volume 33 elocation e031

Resumo: En | Texto: En | PDF: En

Abstract: Variable rates of HPV infection have been reported in healthy oral mucosa worldwide. The main objective of this study was to detect and genotype HPV infection in users and nonusers of drugs with clinically healthy mucosa from the Northeast Brazil. Samples from 105 patients were amplified using the primers MY09/MY11 and GP5+/GP6+, and genotyping was performed by multiplex-PCR for HPV-6/11, 16 and 18. A total of 81.9% samples were positive. Among drug users, 84.5% presented the virus and 20.4% showed multiple infections. Among non-drug users, 78.7% were positive and 13.5% had multiple infections. Limited information is available on oral HPV in Brazilian population, especially for drug users, and our results showed higher HPV infection rates in both users and nonusers of drugs. More studies and researches focused on drug users including factors like sexual behavior, nutrition and cultural habits are necessary to enhance the comprehension of this relationship, and develop preventive strategies.

https://doi.org/10.1590/1807-3107bor-2019.vol33.0031 1081 downloads

11.

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil
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RIBEIRO, Mariana Goveia Melo MARCOLINO, Larissa Doddi RAMOS, Bruna Ribeiro de Andrade MIRANDA, Elaine Alves TRENTO, Cleverson Luciano JAIN, Sona GURGEL, Ricardo Queiroz SILVA, Márcia Guimarães da DOLABELLA, Silvio Santana

Journal of Applied Oral Science

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Fev 2017, Volume 25 Nº 1 Páginas 69 - 74

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Abstract The role of human papillomavirus (HPV) in oral carcinogenesis is still controversial as detection rates of the virus in oral cavity reported in the literature varies greatly. Objective The aim of this study was to evaluate the frequency of HPV infection and its genotypes in patients with oral lesions at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Brazil. Material and Methods We conducted a molecular study with 21 patients (15 females) aged from two to 83 years with clinically detectable oral lesions. Samples were collected through exfoliation of lesions and HPV-DNA was identified using MY09/11 and GP5+/6+ primers. Genotyping was performed by multiplex PCR. Results Benign, premalignant and malignant lesions were diagnosed by histopathology. HPV was detected in 17 samples. Of these, HPV-6 was detected in 10 samples, HPV-18 in four and HPV-16 in one sample. When samples were categorized by lesion types, HPV was detected in two papilloma cases (2/3), five carcinomas (5/6), one hyperplasia (1/1) and nine dysplasia cases (9/11). Conclusion Unlike other studies in the literature, we reported high occurrence of HPV in oral lesions. Further studies are required to enhance the comprehension of natural history of oral lesions.

https://doi.org/10.1590/1678-77572016-0313 2054 downloads

12.

Analysis of evapotranspiration and biomass in pastures with degradation indicatives in the Upper Tocantins River Basin, in Brazilian Savanna
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Andrade, Ricardo Guimarães Teixeira, Antônio Heriberto de Castro Leivas, Janice Freitas Nogueira, Sandra Furlan

Revista Ceres

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Dez 2016, Volume 63 Nº 6 Páginas 754 - 760

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O presente estudo objetivou aplicar o Simple Algorithm For Evapotranspiration Retrieving (SAFER) juntamente com imagens MODIS e dados meteorológicos para analisar a evapotranspiração (ET) e a produção de biomassa (BIO) de acordo com classes indicativas de degradação das pastagens na bacia hidrográfica do Alto Tocantins. Classes indicativas de pastagens degradadas foram obtidas a partir de série temporal de NDVI (2002 a 2012). Para estimar a ET e a BIO em cada classe, imagens MODIS e dados de estações meteorológicas do ano de 2012 foram utilizados. Os resultados indicam que, em relação às pastagens não degradadas, a ET e a BIO apresentaram diferenças, principalmente no período de estresse hídrico, nas pastagens com indicativo de degradação moderado e forte. Assim, alterações na partição do balanço de energia podem ocorrer de acordo com os níveis de degradação, haja vista que indicativos de processos de degradação foram identificados em 24% das áreas de pastagens plantadas. Nesse contexto, as estimativas de ET e BIO por meio de técnicas de sensoriamento remoto pode ser um bom indicador da disponibilidade de forragem e, consequentemente, de aspectos relacionados à degradação das pastagens em larga escala. Espera-se que esse conhecimento possa contribuir para iniciativas de políticas públicas voltadas para o controle da perda do potencial produtivo das áreas de pastagens na bacia do Alto Tocantins, Estado de Goiás.

ABSTRACT The objective of this study was to apply the Simple Algorithm For Evapotranspiration Retrieving (SAFER) with MODIS images together with meteorological data to analyze evapotranspiration (ET) and biomass production (BIO) according to indicative classes of pasture degradation in Upper Tocantins River Basin. Indicative classes of degraded pastures were obtained from the NDVI time-series (2002-2012). To estimate ET and BIO in each class, MODIS images and data from meteorological stations of the year 2012 were used. The results show that compared to not-degraded pastures, ET and BIO were different in pastures with moderate to strong degradation, mainly during water stress period. Therefore, changes in energy balance partition may occur according to the degradation levels, considering that those indicatives of degradation processes were identified in 24% of the planted pasture areas. In this context, ET and BIO estimates using remote sensing techniques can be a reliable indicator of forage availability, and large-scale aspects related to the degradation of pastures. It is expected that this knowledge may contribute to initiatives of public policies aimed at controlling the loss of production potential of pasture areas in the Upper Tocantins River Basin in the state of Goiás, Brazil.

https://doi.org/10.1590/0034-737x201663060002 1294 downloads

13.

Energy and mass transfer parameters in a brazilian semi-arid ecosystem under different thermohydrological conditions
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Teixeira, Antônio Heriberto de Castro Andrade, Ricardo Guimarães Leivas, Janice Freitas

Revista Brasileira de Meteorologia

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Dez 2015, Volume 30 Nº 4 Páginas 381 - 393

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Na região semiárida brasileira, a vegetação natural (Caatinga) tem sido substituída pela agricultura irrigada, enfatizando a importância da quantificação das trocas de massa e de energia. Medições microclimáticas e das correlações turbulentas no ecossistema Caatinga, foram analisadas para dois anos, envolvendo diferentes condições termo hidrológicas. O fluxo de calor sensível (H) representou 49 e 64% do saldo de radiação (Rn), respectivamente durante as condições mais úmidas e mais secas de 2004 e 2005. As frações correspondentes de Rn para o fluxo de calor latente (LE) foram de 40% e 25%. A evapotranspiração (ET) em 2004, com 693 mm, representou 96% da precipitação (P), enquanto que em 2005 (399 mm), foi 18% maior que P, evidenciando o uso da umidade do solo remanescente do ano anterior mais úmido. Todos os parâmetros de transferência das relações solo-água-vegetação-atmosfera analisados foram influenciados pela quantidade de chuvas. Entretanto, a resistência da superfície (rs) foi o mais fortemente afetado pelas condições de umidade do solo, sendo reduzida com o aumento da razão da ET para a evapotranspiração de referência (ET0). Por outro lado, os valores mais elevados de rs foram relacionados com elevações em ambos, do déficit de pressão do vapor (De) e da temperatura aerodinâmica (T0). A pesquisa corrente objetivou a quantificação das trocas de energia e massa entre a Caatinga e a baixa atmosfera, testando em que circunstâncias os parâmetros biofísicos de controle deste processo podem ser estimados razoavelmente com parametrizações através de dados agrometeorológicos, para incorporação em modelos de larga escala.

ABSTRACT In the Brazilian semi-arid region, the natural vegetation ("Caatinga") has been replaced by irrigated agriculture, emphasising the importance for quantification of the energy and mass exchanges. Eddy covariance and micro-climatic measurements in this natural ecosystem, were analysed for two years under different thermohydrological conditions. Sensible heat flux (H) accounted for 49 and 64% of the net radiation (Rn), respectively, during the wetter and the drier conditions of 2004 and 2005. The corresponding fractions of Rn partitioned as latent heat flux (LE) were 40% and 25%. Evapotranspiration (ET) in 2004, with 693 mm, represented 96% of precipitation (P), while in 2005 (399 mm), it was 18% higher than P, which evidenced the use of the remaining soil moisture from the previous wetter year. All the soil-water-vegetation-atmosphere transfer parameters were influenced by the rainfall amounts. However, the surface resistance (rs) was the most strongly affected by the soil moisture status, dropping with increases of the ratio of ET to reference evapotranspiration (ET0). On the other hand, the highest rs values were related to increases in both vapour pressure deficit (De) and aerodynamic temperature (T0). The current research aimed to quantify the energy and mass exchange between the "Caatinga" and the lower atmosphere, testing in which circumstances the biophysical controlling parameters can be reasonably predicted from agrometeorological data, throughout parameterizations, to incorporate in large-scale models.

https://doi.org/10.1590/0102-778620140101 1655 downloads

14.

Estimativa da produtividade da cana-de-açúcar utilizando o Sebal e imagens Landsat
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Andrade, Ricardo Guimarães Sediyama, Gilberto Soares, Vicente Paulo Gleriani, José Marinaldo Menezes, Sady Junior Martins da Costa

Revista Brasileira de Meteorologia

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Set 2014, Volume 29 Nº 3 Páginas 433 - 442

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

As técnicas de sensoriamento remoto têm sido muito promissoras para o desenvolvimento de medidas mais confiáveis e economicamente viáveis da produção vegetal em larga escala. O algoritmo SEBAL (Surface Energy Balance Algorithm for Land) tem como vantagem a obtenção de parâmetros biofísicos usando imagens de satélite e poucos dados observacionais. Este trabalho objetivou estimar a produtividade da cana-de-açúcar por meio da aplicação do algoritmo SEBAL e de imagens Landsat 5 TM. O estudo foi realizado em plantios de cana-de-açúcar da fazenda Boa Fé, localizada no Triângulo Mineiro, município de Conquista, Minas Gerais. A metodologia utilizada apresentou variação de desempenho nas estimativas de produtividade da cana-de-açúcar de cada gleba, provavelmente em decorrência de influências da dimensão dos talhões e da resolução espacial da imagem, e de variedades e épocas de plantio e colheita da cultura. No entanto, os resultados apontam que a metodologia tem potencial para ser aplicada em áreas extensas com limitada disponibilidade de dados meteorológicos.

Remote sensing techniques have shown very promising results in the development of more trustworthy and economically viable large-scale agricultural production measurements. The Surface Energy Balance Algorithm for Land (SEBAL) has the advantage of obtaining biophysical parameters using satellite images and few observational data. This work aimed to estimate sugarcane production using the SEBAL algorithm and Landsat 5 TM images. It was performed on sugarcane crops at the Boa Fé farm, located in Conquista, MG, Brazil. The used method showed varying performance in the sugarcane production estimates for each plot, probably due to the influences of the size of the plots and the spatial resolution of the image, and of varieties and crop planting and harvest dates. However, the results indicate the method has potential for application on large areas on which there is limited availability of meteorological data.

https://doi.org/10.1590/0102-778620130022 4843 downloads Citado 2 vezes em SciELO

15.

Anorectal melanoma - histopathological and immunohistochemical features and treatment
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Cruz, Geraldo Magela Gomes da Andrade Filho, José de Souza Patrus, Gil Leite, Sinara Mônica de Oliveira Silva, Ilson Geraldo da Teixeira, Ricardo Guimarães Braga, Áurea Cassia Gualberto Ferreira, Renata Magali Ribeiro Silluzio

Journal of Coloproctology (Rio de Janeiro)

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Jun 2014, Volume 34 Nº 2 Páginas 95 - 103

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Os melanomas anorretais (ARM) devem ser caracterizados pela localização (anal, retal e anorretal), coloração, dimensão, forma e mobilidade. Microscopicamente, por tipos de melanócitos, graduação da pigmentação melânica, alterações juncionais sob o epitélio escamoso, índice mitótico atípico, atipias celulares e citoplasmáticas, infiltrado inflamatório, invasões vascular e perineural, linfonodo sentinela e penetração parietal. Devem ser estadiados pelos critérios American Joint Committee on Cancer (AJCC) e/ou TNM Classification of Malignant Tumours (TNM). Como as células do ARM são variáveis, isto torna difícil o diagnóstico diferencial com outros tumores da região anorretal. Assim, faz-se necessária a realização de IHC. Apresentamos uma série de 14 pacientes, nos quais foram refeitos exames histológicos e realizados IHCs com vários marcadores, firmando corretamente o diagnóstico. Os trabalhos mostram que a cirurgia mais racional é a excisão local alargada (ELA) em casos de doença localizada e ressecção abdominoperineal do reto (APR) para lesões avançadas. Independente da técnica, a sobrevida de cinco anos é inferior a 35%; a sobrevida média não ultrapassa 26 meses; o tempo livre de doença é inferior a 10 meses; e a sobrevida global não ultrapassa é de 32 meses. Não há correlação entre melhora dos resultados com qualquer tratamento adjuvante. As terapias-alvo para doença metastática começam a apresentar resultados animadores, ainda inconclusivos.

Anorectal melanomas should be characterized by location (anal, rectal and anorectal), color, size, shape and mobility and microscopically, by melanocyte subtypes, grade of melanin pigmentation, junctional changes in the squamous epithelium, atypical mitotic index, cellular atypia, inflammatory infiltrate, vascular and perineural invasion, sentinel lymph node, and anorectal parietal penetration. Anorectal melanomas must be staged by American Joint Committee on Cancer (AJCC) and/or TNM Classification of Malignant Tumours (TNM) criteria. As melanocytes can present with several shapes, sometimes the differential diagnosis with other tumors in this region may be difficult. Because of this, immunohistochemistry is mandatory to attain a precise diagnosis. This study is a report of 14 patients with anorectal melanoma, in whom histological examinations were remade and immunohistochemistry was performed with several markers for melanocytes and for other tumor cells of the anorectal region, properly establishing the diagnosis. The most rational surgery is the extended local resection, when the disease is restricted to the area or the abdominoperineal resection to advanced lesions. Regardless of the technique used, the results are always poor. The authors deny any efficacy of current radio and/or chemotherapy as part of treatment of anorectal melanoma. Target-therapy for metastatic disease has been considered a good strategy, but the results are still inconclusive.

https://doi.org/10.1016/j.jcol.2014.02.006 1438 downloads

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