Pesquisa | SciELO

ABSTRACT Toxoplasmosis is an infection of vast worldwide distribution whose etiologic agent is Toxoplasma gondii. This disease can cause problems ranging from mild symptoms to serious conditions, such as encephalitis, miscarriage and blindness. Therefore, it is of utmost importance to perform a diagnosis with reproducible techniques in order to obtain a good prognosis. The aim of this review was to analyze the efficiency of toxoplasmosis diagnostic techniques based on sensitivity and specificity results. Five research platforms in English language were used (Eric, Elsevier, Google Scholar, PubMed and SciELO), which contained data on the diagnosis of toxoplasmosis. The search and selection were performed for studies published prior to June 2021. The search resulted in the inclusion of 13 articles published from 2005 to 2020. The data revealed the use of different samples in the standardization of techniques such as serum, total blood, colostrum and amniotic fluid. The flow cytometry, lateral flow immunoassay and qPCR techniques showed 100% sensitivity, whereas the ELISA, western blotting, qPCR and RE-LAMP techniques achieved 100% specificity. Significantly, the qPCR and LAMP techniques were more accurate when the likelihood ratio was assessed. The meta-analysis identified that ISAGA and western blotting have low sensitivity values and LIASON, ELFA and ELISA, using a silica bioconjugate, also have low specificity values. It was noted that a wide range of methods have high values of sensitivity and specificity. Therefore, the choice of the method will be based on the conditions and its financial viability.

https://doi.org/10.1590/s1678-9946202365019

The Challenges of Implementing a Text Message Intervention to Promote Behavioral Change in Primary Care Patients With Hypertension and Diabetes
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Oliveira, João Antonio de Queiroz

; Cimini, Christiane

; Almeida, Vania

; Maia, Junia Xavier

; Resende, Raissa Eda

; Gualberto, Leticia Alves

; Santos, Pedro Henrique Lauar

; Gomes, Paulo R.

; Bonisson, Leonardo

; Paixão, Maria Cristina

; Costa, Janaina Moutinho

; Cardoso, Clareci Silva

; Santo, Karla

; Ribeiro, Antonio

; Martins, Maria Auxiliadora

; Marcolino, Milena S.

International Journal of Cardiovascular Sciences

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2023, Volume 36 elocation e20220050

Resumo: En | Texto: En | PDF: En

Abstract Background Controlling blood pressure and glycemic levels is a challenge that requires innovative solutions. Objective To assess the feasibility of implementing a text message intervention among low-income primary care patients, as well as to assess self-reported behavioral change. Methods A set of 200 text messages was developed on healthy eating, physical activity, adherence, and motivation. Participants from Vale do Mucuri, MG, Brazil diagnosed with diabetes or hypertension or undergoing screening for those diseases, received 5 to 8 messages per week for 6 months. They answered a questionnaire to report their satisfaction and behavioral changes. Results Of the 136 patients, 117 (86.0%) answered the questionnaire. Most reported that the messages were very useful (86.3%), easy to understand (90.6%), and were very helpful for behavioral change (65.0%); 84.6% reported that they had started eating healthier. The most frequent reported lifestyle changes were: improved diet quality (85.5%), reduced portions (65.8%), and weight loss (56.4%). The majority of patients shared the messages (60.7%) with family or other acquaintances, considered the number of messages to be adequate (89.7%) and would recommend the program to others (95.7%). Conclusion An intervention based on text messages to promote behavioral change in patients with hypertension or diabetes in primary care is feasible in low-resource settings. Future studies are needed to assess the program’s long-term effects on clinical outcomes.

https://doi.org/10.36660/ijcs.20220050

In vitro Antioxidant and Anticholinesterase Activities of Ouratea fieldingiana (Gardner) Eng. Leaf Extract and Correlation with Its Phenolics Profile with an in silico Study in Relation to Alzheimer’s Disease
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Frota, Lucas S.

; Alves, Daniela R.

; Freitas, Leonardo S.

; Lopes, Francisco F. S.

; Marinho, Marcia M.

; Marinho, Emmanuel S.

; Morais, Selene M. de

Journal of the Brazilian Chemical Society

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Mai 2022, Volume 33 Nº 5 Páginas 446 - 455

Resumo: En | Texto: En | PDF: En

Ouratea fieldingiana is a native medicinal plant from Northeastern Brazil and many biological properties are due to the phenolic constituents. The objective of this work was performing the characterization of O. fieldingiana leaf constituents to correlate with antioxidant and anticholinesterase activities by in vitro and in silico studies and thus contribute to find new agents against Alzheimer’s disease. The high-performance liquid chromatography revealed the presence of the flavonoids rutin, isoquercitrin, kaempferol-3-O-rutinoside, quercetin, apigenin and amentoflavone. The antioxidant activities by the (2,2-diphenyl-1-picrylhydrazyl) (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methodologies, showed good results with half maximal inhibitory concentration (IC50) values ranging from 5.63 to 11.47 µg mL-1 and 2.72 to 23.71 µg mL-1, respectively. Acetylcholinesterase inhibition assay pointed out the flavone apigenin with best activity. Computational studies evaluated the interaction of flavonoids with the enzyme acetylcholinesterase co-crystallized with the galantamine, used as standard. All flavonoids exhibited binding energy greater than that of galantamine, but only apigenin showed strong interaction with the active site of the enzyme and other bind probably to different allosteric centers. Then, O. fieldingiana extract and flavonoids with good anti-radical activity and presenting a broad-spectrum action against acetylcholinesterase (AChE) enzyme ought to be tested in clinical studies to discover new neuro-therapeutic candidates.

https://doi.org/10.21577/0103-5053.20210163

Hybrid Quaternions of Leonardo
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MANGUEIRA, M. C. dos S.

; ALVES, F. R. V.

; CATARINO, P. M. M. C.

Trends in Computational and Applied Mathematics

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Jan 2022, Volume 23 Nº 1 Páginas 51 - 62

Resumo: En | Texto: En | PDF: En

ABSTRACT In this article, we intend to investigate the Leonardo sequence presenting the hybrid Leonardo quaternions. To explore Hybrid Quaternions of Leonardo, the priori, sequence of Leonardo, quaternions and hybrid numbers were presented. Soon after, its recurrence, characteristic equation, its relation with the Fibonacci quaternions, generating function, Binet’s formula, as well as its extension to non-positive integer indices were developed. Finally, identities involving Leonardo’s hybrid quaternions are presented.

https://doi.org/10.5540/tcam.2022.023.01.00051

Fire ants: What do rural and urban areas show us about occurrence, diversity, and ancestral state reconstruction?
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Ramalho, Manuela de O.

; Menino, Leonardo

; Souza, Rodrigo F.

; Kayano, Débora Y.

; Alves, Juliana M. C.

; Harakava, Ricardo

; Nagatani, Victor H.

; Silva, Otávio G. M.

; Bueno, Odair C.

; Morini, Maria S. C.

Genetics and Molecular Biology

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2022, Volume 45 Nº 1 elocation e20210120

Resumo: En | Texto: En | PDF: En

Abstract In South America, Solenopsis saevissima and S. invicta are the most common fire ants. Nests are founded in areas under anthropic interference like urban or rural areas, but S. invicta is found preferentially in those with the greatest anthropic interference. However, we do not know the rates at which they exist in anthropized areas next to high density of native vegetation. Areas with 60 to 90% of native Atlantic Forest were selected to verify the occurrence of both species in rural and urban areas. We investigated the molecular diversity and applied the reconstruction of the ancestral state analysis for each species. A total of 186 nests were analyzed and we found that the two species had the same proportion in the urban area. However, S. saevissima had a higher rate of prevalence in the rural area, in addition to having a greater number of haplotypes and ancestry associated with this type of habitat for the region. S. invicta had the same number of haplotypes in both rural and urban regions, and less haplotypic diversity. We conclude that S. saevissima is a species typically associated with rural areas and S. invicta, although present, is not dominant in urban areas.

https://doi.org/10.1590/1678-4685-gmb-2021-0120

EL GÉNERO CYPELLA (IRIDACEAE) EN URUGUAY
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Deble, Leonardo Paz

; González, Andrés

; Alves, Fabiano da Silva

Darwiniana, nueva serie

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Jun 2021, Volume 9 Nº 1 Páginas 173 - 208

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Deble, L. P.; A. González & F. da S. Alves. 2021. The genus Cypella (Iridaceae) in Uruguay. Darwiniana, nueva serie 9(1): 173-208. The genus Cypella is studied for the Uruguayan Flora. Based in revision of Herbaria and collections sampling were recognized 13 species and five subspecies for the country: C. alonsoana, C. aurinegra subsp. aurinegra, C. aurinegra subsp. albipurpurata, C. charruana, C. discolor, C. exilis, C. fucata, C. guttata, C. herbertii subsp. herbertii, C. herbertii subsp. brevicristata, C. herbertii subsp. reflexa, C. laeta, C. laxa, C. luteogibbosa, C. osteniana and C. rivularis. Cypella aurinegra subsp. albipurpurata comprises a new subspecies endemic in rocky place, on Mountain Range in Cerro Largo Department, Northeast Uruguay. Cypella laxa is a new record for the country. It is supplied dichotomous key, descriptions, material examined, comments and photos for all taxa identified. Furthermore, ecological data, conservation status, excluded species and other taxa with possible occurrence in Uruguay are listed.

Resumen Deble, L. P.; A. González & F. da S. Alves. 2021. El género Cypella (Iridaceae) en Uruguay. Darwiniana, nueva serie 9(1): 173-208. El género Cypella es investigado para la Flora Uruguaya. Basado en revisión de herbario y estudios en el campo fueron reconocidas 13 especies y cinco subespecies de Cypella: C. alonsoana, C. aurinegra subsp. aurinegra, C. aurinegra subsp. albipurpurata, C. charruana, C. discolor, C. exilis, C. fucata, C. guttata, C. herbertii subsp. herbertii, C. herbertii subsp. brevicristata, C. herbertii subsp. reflexa, C. laeta, C. lapidosa, C. luteogibbosa, C. osteniana y C. rivularis. Cypella aurinegra subsp. albipurpurata consiste en una nueva subespecie endémica en locales rocosos de las serranías del Departamento de Cerro Largo. Cypella laxa es un nuevo registro para el país. Se presenta clave dicotómica, descripciones, material examinado, comentarios y fotos para todos los taxones. Además, observaciones ecológicas, estado de conservación, especies excluidas y otros taxones con posible presencia en Uruguay son comentados.

https://doi.org/10.14522/darwiniana.2021.91.921 115 downloads

USE OF INOCULUM IN BIODIGESTERS WITH CATTLE MANURE UNDER CONVENTIONAL AND ORGANIC PRODUCTION SYSTEMS
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Paes, Juliana L.

; Alves, Thaís B. S.

; Silva, Leonardo D. B. da

; Marques, Anieli de S.

; Dias, Valentina R. S.

Engenharia Agrícola

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Abr 2020, Volume 40 Nº 2 Páginas 146 - 153

Resumo: En | Texto: En | PDF: En

ABSTRACT One way of making livestock farming a more sustainable practice is to raise cattle organically. However, regardless of the production system, there is a generation of residues that can be converted into energy resources using biodigesters. This study aimed to evaluate the addition of inoculum in the anaerobic biodigestion of cattle manure under organic (OPS) and conventional production systems (CPS) in an Indian model biodigester. The physicochemical (pH, EC, TS, VS, DBO, and COD) and microbiological characteristics of the affluent and effluent, as well as the production, potential, and qualitative characteristics of the biogas, were evaluated. CPS showed higher reductions in TS, VS, BOD, and COD than OPS, with the highest effect of biogas production. Regarding the effect of inoculum addition, the treatment with 20% inoculum provided in both production systems high acceleration at the starting point of biogas production and increased energy potential, as well as high reductions in VS, BOD, and COD. Thus, there is a need to use 20% inoculum for high biogas productivity, regardless of the production system.

https://doi.org/10.1590/1809-4430-eng.agric.v40n2p146-153/2020 967 downloads

HPLC-PDA method validated for the determination of hibalactone in Hydrocotyle umbellata subterraneous parts and its ultrasound-assisted extraction optimization
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Oliveira, Matheus G. de

; Almeida, Paulo H.G. de

; Oliveira, Thiago L.S.

; Silva, Liliane de S.

; Carvalho, Flávio S. de

; Alves, Suzana F.

; Borges, Leonardo L.

; Santos, Pierre A. dos

; Silva, Vinicius B. da

; Paula, José R. de

Revista Brasileira de Farmacognosia

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Abr 2019, Volume 29 Nº 2 Páginas 162 - 170

Resumo: En | Texto: En | PDF: En

ABSTRACT In this work, we developed and validated a HPLC-PDA method for the quantification of hibalactone in Hydrocotyle umbellata L., Araliaceae, subterraneous parts extracts and optimized its ultrasound-assisted extraction. Chromatographic separations were carried out with an isocratic mobile phase of acetonitrile/methanol/water (10:65:25), a flow of 0.8 ml min−1, detection at 290 nm and C18 column (250 × 4.6 mm, 5 µm). The method validation parameters were determined according to Brazilian legislation. The optimization of the hibalactone ultrasound-assisted extraction was performed using Box–Behnken design and response surface methodology. The HPLC method for hibalactone quantification proved to be selective, linear, precise, accurate and robust, being useful for the analysis of hibalactone in H. umbellata subterraneous parts extracts. The optimal ultrasound-assisted extraction conditions were obtained with solid-to-liquid ratio of 1:5 g ml−1, ethanolic strength of 70% (v/v) and temperature of 65 °C. The results can provide support of the quality control and standardization of raw materials from H. umbellata.

https://doi.org/10.1016/j.bjp.2018.10.003 572 downloads

Medidas cepstrais na avaliação da intensidade do desvio vocal
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Lopes, Leonardo Wanderley

; Sousa, Estevão Silvestre da Silva

; Silva, Allan Carlos França da

; Silva, Itacely Marinho da

; Paiva, Maxsuel Avelino Alves de

; Vieira, Vinícius Jefferson Dias

; Almeida, Anna Alice

CoDAS

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2019, Volume 31 Nº 4 elocation e20180175

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo Analisar se existe associação entre a presença, a intensidade e o tipo de desvio vocal e as medidas cepstrais em amostras de indivíduos com queixa vocal. Método Foram utilizadas 376 amostras da vogal /ε/ de indivíduos de ambos os gêneros, com queixa vocal. Utilizou-se uma escala analógico-visual para análise perceptivo-auditiva das vozes quanto à intensidade do desvio vocal (GG), graus de rugosidade (GR), soprosidade (GS) e tensão (GT), incluindo-se a determinação da qualidade vocal predominante (rugosa, soprosa ou tensa). Foram extraídas as medidas relacionadas ao Cepstral Peak Prominence-Smoothed (CPPS) e o declínio espectral das amostras vocais. Resultados Houve diferença dos valores do CPPS entre os grupos com e sem desvio vocal, assim como entre as diferentes intensidades e tipos de desvio vocal. Os valores do CPPS foram mais reduzidos em função da presença e intensidade do desvio vocal. Os valores do CPPS diferenciaram vozes rugosas x soprosas, rugosas x tensas e soprosas x tensas. O declínio espectral apenas diferenciou vozes soprosas x tensas. O CPPS se correlacionou de modo positivo e forte com os GG e GS, de modo negativo moderado com o GR, e de forma negativa fraca com o GT. O declínio espectral apresentou correlação positiva moderada com o GT e correlação negativa fraca com o GS. Conclusão Existe associação entre a presença de desvio vocal, o GG, a qualidade vocal predominante e o CPPS. De modo especial, o GG é fortemente correlacionado ao CPPS. O declínio espectral está associado apenas aos parâmetros de soprosidade e tensão.

ABSTRACT Purpose To analyze whether there is an association between the presence, intensity and type of voice disorder and the cepstral measures in samples of individuals with voice complaints. Methods We used 376 vowel /Ɛ/ samples from individuals of both genders that had voice complaints. An analogue-visual scale was used for the auditory-perceptual analysis of voices regarding the overall grade of dysphonia (G) and the grades of roughness (R), breathiness (B), and strain (S), including a determination of voice quality (rough, breathy or strained). Measures related to cepstral peak prominence smoothed (CPPS) and spectral decline of vocal samples were extracted. Results There were differences in the CPPS values between the groups with or without voice disorders as well as between the different intensities and types of voice disorder. CPPS values were lower because of the presence and intensity of voice disorders. The CPPS values differentiated the following voices: rough x breathy, rough x strained, and breathy x strained. The spectral decline only differentiated breathy x strained voices. CPPS correlated positively and strongly with G and B; moderately and negatively with R, and negatively and weakly with S. The spectral decline had a moderate positive correlation with S and a weak negative correlation with B. Conclusion There is association between voice disorder, G, predominant voice quality, and CPPS. In particular, G is strongly correlated with CPPS. Spectral decline is associated only with the parameters B and S.

https://doi.org/10.1590/2317-1782/20182018175 2954 downloads

10.

Phytochemistry and antimicrobial activity of Campomanesia adamantium
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Sá, Stone

; Chaul, Luíza T.

; Alves, Virgínia F.

; Fiuza, Tatiana S.

; Tresvenzol, Leonice M.F.

; Vaz, Boniek G.

; Ferri, Pedro H.

; Borges, Leonardo L.

; Paula, José R.

Revista Brasileira de Farmacognosia

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Jun 2018, Volume 28 Nº 3 Páginas 303 - 311

Resumo: En | Texto: En | PDF: En

ABSTRACT Campomanesia adamantium (Cambess.) O. Berg., Myrtaceae, is a plant popularly used for its anti-inflammatory, anti-diarrhoeal and urinary antiseptic activities. The aims of this study were to obtain the crude ethanolic extract and the hexane, dichloromethane, ethyl acetate, aqueous and concentrated aqueous tannin fractions from C. adamantium leaves, perform biomonitored fractionation to isolate and identify chemical compounds, study the chemical composition of the volatile oils of the leaves and flowers and test the antimicrobial activity of the ethanolic extract, fractions, isolated substances and volatile oils. Phytochemical screening and chromatographic and spectrometric techniques were used. Volatile oils were isolated by hydrodistillation in a Clevenger apparatus and analyzed by gas chromatography/mass spectrometry. The antimicrobial activity was tested by a broth microdilution test. The component stictane-3,22-diol was isolated and identified from the hexane fraction, while valoneic and gallic acid were isolated and identified from the concentrated aqueous tannin fraction. The major constituents of the volatile oils of the leaves were verbenene (13.91%), β-funebrene (12.05%) and limonene (10.32%), while those of the volatile oils of the flowers were sabinene (20.45%), limonene (19.33%), α-thujene (8.86%) and methyl salicylate (8.66%). Antibacterial activity was verified for the hexane fraction, while antifungal activity was observed for the aqueous fraction and concentrated aqueous tannin fraction and for vanoleic acid. These results may justify the popular use of C. adamantium.

https://doi.org/10.1016/j.bjp.2018.02.008 902 downloads

11.

D-loop haplotype diversity in Brazilian horse breeds
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Ianella, Patrícia

; Albuquerque, Maria do Socorro Maués

; Paiva, Samuel Rezende

; Egito, Andréa Alves do

; Almeida, Leonardo Daniel

; Sereno, Fabiana T. P. S.

; Carvalho, Luiz Felipe Ramos

; Mariante, Arthur da Silva

; McManus, Concepta Margaret

Genetics and Molecular Biology

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Set 2017, Volume 40 Nº 3 Páginas 604 - 609

Resumo: En | Texto: En | PDF: En

Abstract The first horses were brought to Brazil by the colonizers after 1534. Over the centuries, these animals evolved and adapted to local environmental conditions usually unsuitable for exotic breeds, thereby originating locally adapted Brazilian breeds. The present work represents the first description of maternal genetic diversity in these horse breeds based on D-loop sequences. A D-Loop HSV-I fragment of 252 bp, from 141 horses belonging to ten Brazilian breeds / genetic groups (locally adapted and specialized breeds) were analysed. Thirty-five different haplotypes belonging to 18 haplogroups were identified with 33 polymorphic sites. Haplotype diversity (varying from 0.20 to 0.96) and nucleotide diversity (varying from 0.0039 to 0.0239) was lower for locally adapted than for specialized breeds, with the same pattern observed for FST values. Haplogroups identified in Brazilian breeds are in agreement with previous findings in South American samples. The low variability observed mainly in locally adapted breeds, indicates that, to ensure conservation of these breeds, careful reproductive management is needed. Additional genetic characterization studies are required to support accurate decision-making.

https://doi.org/10.1590/1678-4685-gmb-2016-0166 1144 downloads

12.

Lack of association between viral load and severity of acute bronchiolitis in infants
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Souza, Ana Paula Duarte de

; Leitão, Lidiane Alves de Azeredo

; Luisi, Fernanda

; Souza, Rodrigo Godinho

; Coutinho, Sandra Eugênia

; Silva, Jaqueline Ramos da

; Mattiello, Rita

; Pitrez, Paulo Márcio Condessa

; Stein, Renato Tetelbom

; Pinto, Leonardo Araújo

Jornal Brasileiro de Pneumologia

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Ago 2016, Volume 42 Nº 4 Páginas 261 - 265

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Investigar a correlação entre a carga viral do vírus sincicial respiratório e o tempo de internação hospitalar em lactentes com episódios de sibilância aguda. Métodos: Este foi um estudo transversal de dois anos envolvendo lactentes de até 12 meses de idade com bronquiolite no momento da internação em um hospital terciário. Para a identificação dos vírus respiratórios foram coletadas secreções nasofaríngeas. As amostras foram analisadas (por todo o período do estudo) por imunofluorescência direta e (no segundo ano do estudo) por PCR quantitativa em tempo real para três vírus humanos (rinovírus, vírus sincicial respiratório e metapneumovírus). Resultados: Das 110 amostras avaliadas por imunofluorescência direta, 56 (50,9%) foram positivas para um único vírus, e 16 (14,5%) foram positivas para dois ou mais vírus. Nessas 72 amostras, o vírus mais prevalente foi o vírus sincicial respiratório, seguido por influenza. Das 56 amostras avaliadas por PCR quantitativa em tempo real, 24 (42,8%) foram positivas para um único vírus, e 1 (1,7%) foi positiva para dois vírus. Nessas 25 amostras, o vírus mais prevalente foi o vírus sincicial respiratório, seguido por rinovírus humano. A coinfecção não influenciou o tempo de internação ou outros desfechos. Além disso, não houve associação entre a carga viral de vírus sincicial respiratório e o tempo de internação. Conclusões: A coinfecção e a carga viral do vírus sincicial respiratório não parecem influenciar os desfechos em lactentes com bronquiolite aguda.

ABSTRACT Objective: To investigate the correlation between respiratory syncytial viral load and length of hospitalization in infants with acute wheezing episodes. Methods: This was a two-year, cross-sectional study of infants ≤ 12 months of age with bronchiolitis at the time of admission to a tertiary hospital. For the identification of respiratory viruses, nasopharyngeal secretions were collected. Samples were analyzed (throughout the study period) by direct immunofluorescence and (in the second year of the study) by quantitative real-time PCR. We screened for three human viruses: rhinovirus, respiratory syncytial virus, and metapneumovirus. Results: Of 110 samples evaluated by direct immunofluorescence, 56 (50.9%) were positive for a single virus, and 16 (14.5%) were positive for two or more viruses. Among those 72 samples, the most prevalent virus was respiratory syncytial virus, followed by influenza. Of 56 samples evaluated by quantitative real-time PCR, 24 (42.8%) were positive for a single virus, and 1 (1.7%) was positive for two viruses. Among those 25 samples, the most prevalent virus was again respiratory syncytial virus, followed by human rhinovirus. Coinfection did not influence the length of the hospital stay or other outcome s. In addition, there was no association between respiratory syncytial virus load and the length of hospitalization. Conclusions: Neither coinfection nor respiratory syncytial viral load appears to influence the outcomes of acute bronchiolitis in infants.

https://doi.org/10.1590/s1806-37562015000000241 3010 downloads

13.

Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist
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Lima, Leonardo R. de

; Mendes, Heloisa M.F.

; Soriani, Frederico M.

; Souza, Danielle G. de

; Alves, Geraldo Eleno S.

; Teixeira, Mauro M.

; Faleiros, Rafael R.

Pesquisa Veterinária Brasileira

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Jan 2016, Volume 36 Nº 1 Páginas 13 - 18

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: A expressão de quimiocinas e a infiltração de leucócitos no tecido laminar são característicos de laminite aguda de equinos. O presente estudo avaliou o efeito terapêutico da administração intravenosa de DF1681B , um antagonista seletivo para CXCR1 e CXCR2 (receptores de quimiocinas), em um modelo de laminite equina por oligofrutose. Utilizaram-se doze cavalos sem raça definida, compreendendo quatro machos e oito fêmeas não gestantes, com idade (média ±SD) 7±3,5 anos, pesando 305±35kg e com uma pontuação média de condição corporal de 5±1/9. Os indivíduos elegíveis eram clinicamente saudáveis, sem história prévia de claudicação relacionados ao casco. Após administração de oligofrutose (10g/kg por sonda nasogástrica), os animais foram divididos em dois grupos: tratado (30mg/kg de DF1681B intravenosa, 6, 12, 18 e 24h após a oligofrutose) e não tratado, que recebeu placebo. Biópsias laminares foram realizadas antes e 12, 36 e 72h após a administração de oligofrutose. As amostras foram coradas com ácido periódico de Schiff (PAS) e classificadas de 0-6 de acordo com alterações nas células epidérmicas e na membrana basal. Também determinaram-se as expressões gênicas de IL-1β, CXCL1 e IL-6 por RT-PCR. Testes paramétricos e não paramétricos foram utilizados para comparar os momentos em cada grupo (P<0,05). Estatisticamente, os graus PAS e as expressões de IL-1β e IL-6 se elevaram após a indução no grupo não tratado, mas se mantiveram constantes nos cavalos tratados. Em conclusão, a terapia por DF1681B reduziu a inflamação laminar e a deterioração epidérmica em equinos submetidos ao modelo de intoxicação por oligofructose. O bloqueio de receptores CXCR1/2 deve ser considerado como uma opção terapêutica para prevenção da laminite aguda de equinos.

Abstract: With the hypothesis that blocking chemokine signaling can ameliorate acute laminitis, the aim was to evaluate the therapeutic effect of intravenous DF1681B, a selective antagonist for CXCR1 and CXCR2 (chemokine receptors), in an oligofructose equine laminitis model. To twelve mixed breed clinically healthy hoses with no previous history of hoof-related lameness was administered oligofructose (10g/kg given by nasogastric tube) and divided into two groups: treated (intravenous DF1681B at 30mg/kg 6, 12, 18, and 24h after oligofructose) and non-treated groups. Laminar biopsies were performed before and 12, 36, and 72h after administering oligofructose. Samples were stained with periodic acid-Schiff (PAS) and scored from 0 to 6 according to epidermal cell and basal membrane changes. The IL-1β, IL-6, and CXCL1 RNA expressions were determined by RT-PCR. Parametric and non-parametric tests were used to compare times within each group (P<0.05). The PAS grades and IL-1β and IL-6 RNA expression increased in the non-treated group, but remained constant in the treated horses. In conclusion, DF1681B therapy reduced laminar inflammation and epidermal deterioration in treated horses. CXCR1/2 blockage should be considered therapeutically for equine acute laminitis.

https://doi.org/10.1590/S0100-736X2016000100002 1685 downloads

14.

DENDROCRONOLOGIA DE ÁRVORES DE Schizolobium parahyba(Vell.) S. F. Blake DE OCORRÊNCIA NA REBIO DE TINGUÁ-RJ
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Latorraca, João Vicente de Figueiredo

; Souza, Milene Teixeira de

; Silva, Leonardo Davi Silveira Augusto Baptista da

; Ramos, Letícia Maria Alves

Revista Árvore

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Abr 2015, Volume 39 Nº 2 Páginas 385 - 394

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O Schizolobium parahyba (Vell.) Blake, popularmente conhecido por “guapuruvu”, apresenta anéis de crescimento distintos, evidenciados por maior espessamento de suas paredes no lenho tardio e pela presença de parênquima em faixa marginal. Neste contexto, o objetivo deste estudo foi construir a cronologia dos anéis de crescimento de árvores de S. parahyba de ocorrência na ReBio de Tinguá, RJ, visando gerar conhecimento sobre a dinâmica de crescimento da espécie, bem como sobre a sensibilidade da formação dos anéis de crescimento por fatores climáticos. Das 30 árvores selecionadas foram coletadas quatro amostras radiais do tronco, utilizando-se uma sonda Pressler. As amostras passaram por polimento mecânico para melhor visualização dos anéis de crescimento e posterior delimitação e mensuração da largura deles. Para verificar a sincronização da largura dos anéis de crescimento e gerar uma série mestra da cronologia para a espécie, foi utilizado o programa estatístico COFECHA. A espécie apresenta ótimo potencial dendrocronológico, confirmado por elevada correlação da largura dos anéis de crescimento dentre e entre árvores. Além disso, exibe elevado coeficiente de sensibilidade média, que demonstra resposta às variações ambientais. O crescimento da espécie é correlacionado com a precipitação na estação seca.

The Schizolobium parahyba, popularly known as “guapuruvu”, has distinct growth rings evidenced by increased thickening of the walls in the late wood and the presence of marginal parenchyma bands. In this context, the aim of this study was to construct a chronology of the growth rings of S. parahybatrees, occurring in Biological Reserve Tinguá, RJ, in order to generate knowledge about the growth dynamic of the species, as well as the sensitivity of the formation of growth rings by climatic factors. From the 30 selected trees, four radial trunk samples were collected using a Pressler borer. The samples suffered mechanical polishing for better visualization of the growth rings and subsequent delineation and measurement of the width of these rings. The statistical program COFECHA was used to check the synchronization of the width of growth rings and generate a series of master chronology of the specie. The species has a great dendrochronological potential, which was confirmed by a high correlation of the width of growth rings within and between trees. Moreover, the species has a high average sensitivity coefficient, which shows a response to environmental variations. The growth of the species is correlated with precipitation in the dry season.

https://doi.org/10.1590/0100-67622015000200018 4016 downloads

15.

Brazilian consensus on guidelines for diagnosis and treatment for restless legs syndrome
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Fröhlich, Alan Christmann

; Eckeli, Alan Luiz

; Bacelar, Andrea

; Poyares, Dalva

; Pachito, Daniela Vianna

; Stelzer, Fernando Gustavo

; Coelho, Fernando Morgadinho Santos

; Rizzo, Geraldo Nunes Vieira

; Prado, Gilmar Fernandes do

; Sander, Heidi Haueisen

; Goulart, Leonardo Ierardi

; Lucchesi, Ligia Mendonça

; Gitai, Lívia Leite Góes

; Prado, Lucila Bizari Fernandes do

; Ataíde-Junior, Luiz

; Bezerra, Marcio Luciano de Souza

; Lopes, Maria Cecília

; Trentin, Marine Meliksetyan

; Rodrigues, Raimundo Nonato Delgado

; Hasan, Rosa

; Alves, Rosana S. Cardoso

; Schönwald, Suzana Veiga

; Moraes, Walter André dos Santos

Arquivos de Neuro-Psiquiatria

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Mar 2015, Volume 73 Nº 3 Páginas 260 - 280

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O Consenso em síndrome das pernas inquietas contou com a participação de neurologistas de vários estados brasileiros, os quais incansavelmente revisaram a literatura dos últimos anos em busca de evidências, tanto no que se refere ao diagnóstico como ao tratamento, de acordo com a Classificação do Centro de Oxford para Medicina Baseada em Evidências.

The Consensus on restless legs syndrome is an effort of neurologists from several Brazilian states, which tirelessly reviewed the literature of recent years in search of evidence, both in regard to diagnosis and treatment, according to the Oxford Centre for Evidence-based Medicine.

https://doi.org/10.1590/0004-282X20140239 7813 downloads

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