ABSTRACT Despite being subject to lower AIDS-related mortality rates and having a higher life expectancy, patients with HIV are more prone to develop non-AIDS events. A low CD4+/CD8+ ratio during antiretroviral therapy identifies people with heightened immune senescence and increased risk of mortality. In clinical practice, finding determinants of a low CD4+/CD8+ ratio may be useful for identifying patients who require close monitoring due to an increased risk of comorbidities and death. We performed a prospective study on the evolution of the CD4+/CD8+ ratio in 60 patients infected with HIV (80% males), who were subjected to two different antiretroviral regimens: early and deferred therapy. The initial CD4+/CD8+ ratio was ≤1 for 70% of the patients in both groups. Older age, CD4+ cell count at inclusion, Nadir CD8+T-cell count, and Initial CD4+/CD8+ ratio ≤ 1 were risk factors for lack of ratio recovery. In the multivariate analysis, a CD4+/CD8+ ratio > 1 at the start of the treatment was found to be a determinant factor in maintaining a CD4+/CD8+ ratio > 1. The nadir CD4+T-cell count was lower in the deferred therapy group (p=0.004), and the last CD4+/CD8+ ratio ≤1 was not associated with comorbidities. Ratio recovery was not associated with the duration of HIV infection, time without therapy, or absence of AIDS incidence. A greater improvement was observed in patients treated early (p=0.003). In contrast, the slope of increase was slower in patients who deferred treatment. In conclusion, the increase in the CD4+/CD8+ ratio occurred mostly for patients undergoing early strategy treatment and its extension did not seem to be related to previous HIV-related factors. AIDSrelated expectancy nonAIDS non events CD4CD8 CDCD CD4 CD8 CD CD4+/CD8 practice death 6 80% 80 (80 males, males , males) regimens 70 groups age inclusion CD8+Tcell CD8Tcell CDTcell CD8+T T analysis CD4+Tcell CD4Tcell CD4+T p=0.004, p0004 p p=0.004 0 004 (p=0.004) infection incidence p=0.003. p0003 p=0.003 . 003 (p=0.003) contrast conclusion HIVrelated CD4CD CD4+/CD 8 (8 7 Tcell CD8T CDT CD4T p000 p=0.00 00 (p=0.004 (p=0.003 ( p00 p=0.0 (p=0.00 p0 p=0. (p=0.0 p=0 (p=0. p= (p=0 (p= (p

ABSTRACT The sense of mastery is conceptualized as a positive aspect of care targeted at people living with dementia, a coping mechanism to reduce burden, and may represent a protective factor for caregivers’ mental and physical health. Objective: To translate and culturally adapt the Pearlin Mastery Scale for Brazil. Methods: A methodological study was conducted at the Federal University of São Carlos in which the initial translation stages were followed; synthesis; back-translation; review by the committee of judges by analyzing the Content Validity Index (CVI); and test of the pre-final version. Results: Two specialists translated the scale into Brazilian Portuguese and defined a consensus version with the researchers. Subsequently, another two specialists back-translated the consensus version, which was reviewed by three judges who are PhDs in the area, considering all scale items as very equivalent (CVI=1.0), and maintaining them in the pre-final version of the instrument. This was tested in a first group of caregivers for them to point out adjustments. The suggestions were accepted by modifying three items and, afterward, the scale was tested in a second group, which did not present difficulties answering the instrument. Conclusion: The Pearlin Mastery Scale was translated and culturally adapted for Brazil, showing equivalence. However, future psychometric analyses of the instrument are required to make it available for use in this population. dementia burden health Objective Brazil Methods followed synthesis backtranslation back back-translation CVI (CVI) prefinal pre final Results researchers Subsequently backtranslated area CVI=1.0, CVI10 CVI=1.0 , 1 0 (CVI=1.0) adjustments afterward Conclusion equivalence However population (CVI CVI1 CVI=1. (CVI=1.0 CVI=1 (CVI=1. CVI= (CVI=1 (CVI=

RESUMO O senso de domínio é conceituado como um aspecto positivo do cuidado voltado às pessoas que vivem com demência, um mecanismo de enfrentamento para reduzir a sobrecarga e que pode representar um fator protetor para a saúde mental e física do cuidador. Objetivo: Traduzir e adaptar culturalmente a Pearlin Mastery Scale para o Brasil. Métodos: Estudo metodológico conduzido na Universidade Federal de São Carlos, em que foram seguidas as etapas de tradução inicial; síntese; retrotradução; revisão pelo comitê de juízes pela análise do Índice de Validade de Conteúdo (IVC); e teste da versão pré-final. Resultados: Dois especialistas traduziram a escala para o português brasileiro e definiram uma versão consensual com os pesquisadores. Posteriormente, outros dois especialistas retrotraduziram a versão consensual, que foi revisada por três juízes doutores na área, considerando todos os itens da escala como muito equivalentes (IVC=1,0), mantendo-os na versão pré-final do instrumento. Esta foi testada em um primeiro grupo de cuidadores, a fim de se apontarem adequações. As sugestões foram acatadas mediante a modificação de três itens e, depois, a escala foi testada em um segundo grupo, o qual não apresentou dificuldades em responder ao instrumento. Conclusão: A Pearlin Mastery Scale foi traduzida e adaptada culturalmente para o Brasil, demonstrando equivalência. Entretanto, análises psicométricas futuras do instrumento são necessárias para disponibilizá-lo para uso entre essa população. demência cuidador Objetivo Brasil Métodos Carlos inicial síntese retrotradução IVC (IVC) préfinal. préfinal pré final. final Resultados pesquisadores Posteriormente área IVC=1,0, IVC10 IVC=1,0 , 1 0 (IVC=1,0) mantendoos mantendo cuidadores adequações depois Conclusão equivalência Entretanto disponibilizálo disponibilizá lo população (IVC IVC1 IVC=1, (IVC=1,0 IVC=1 (IVC=1, IVC= (IVC=1 (IVC=

ABSTRACT Objective: To conduct a serological screening for toxoplasmosis in the heel prick test and to evaluate its epidemiological aspects in newborns and postpartum women in Jataí, Goiás. Method: Cross-sectional epidemiological study for the biological screening of newborns in Jataí, Goiás. Results: The study participants amounted to 228 newborns, whose samples were collected between the third and seventh day of life. IgG antibodies against Toxoplasma gondii were detected in 40.79% (93/228) of the samples; out of these, 23.6% (22/93) had high IgG antibody titers, leading to the collection of two other peripheral blood samples and the detection of a decrease in these titers. Conclusion: The findings show the importance of strengthening actions in primary health care to prevent infection and training health professionals in this area to equip them with information regarding cases of reinfection and reactivation of infection in pregnant women, minimizing risks for babies. Objective Jataí Goiás Method Crosssectional Cross sectional Results 22 life 4079 40 79 40.79 93/228 93228 93 (93/228 236 23 6 23.6 22/93 2293 (22/93 titers Conclusion babies 2 407 4 7 40.7 93/22 9322 9 (93/22 23. 22/9 229 (22/9 40. 93/2 932 (93/2 22/ (22/ 93/ (93/ (22 (93 (2 (9 (

RESUMO Objetivo: Realizar a triagem sorológica da toxoplasmose no teste do pezinho e avaliar os aspectos epidemiológicos em recém-nascidos e puérperas de Jataí, Goiás. Método: Estudo epidemiológico transversal para a triagem biológica de recém-nascidos em Jataí, Goiás. Resultados: Participaram desta pesquisa 228 recém-nascidos, sendo coletadas amostras entre o terceiro e o sétimo dia de vida. Foram detectados anticorpos IgG anti Toxoplasma gondii em 40,79% (93/228) das amostras; destes, 23,6% (22/93) apresentaram altos títulos de anticorpos IgG e tiveram outras duas amostras de sangue periférico coletadas, sendo verificada a diminuição desses títulos. Conclusão: Tais achados demonstraram a importância do fortalecimento de ações junto à atenção primária à saúde para a prevenção da infecção, assim como a capacitação de profissionais da saúde que atuam nesta área, para que sejam munidos de informações referentes a casos de reinfecção e reativação da infecção em gestantes, minimizando os riscos para os bebês. Objetivo recémnascidos recém nascidos Jataí Goiás Método Resultados 22 recémnascidos, nascidos, vida 4079 40 79 40,79 93/228 93228 93 (93/228 destes 236 23 6 23,6 22/93 2293 (22/93 Conclusão área gestantes bebês 2 407 4 7 40,7 93/22 9322 9 (93/22 23, 22/9 229 (22/9 40, 93/2 932 (93/2 22/ (22/ 93/ (93/ (22 (93 (2 (9 (

RESUMEN Objetivo: Realizar un cribado serológico de la toxoplasmosis en la prueba del talón y evaluar sus aspectos epidemiológicos en los recién nacidos y puérperas en Jataí, Goiás. Método: Estudio epidemiológico transversal para el cribado biológico de los recién nacidos en Jataí, Goiás. Resultados: Participaron en el estudio 228 recién nacidos, cuyas muestras fueron recolectadas entre el tercer y séptimo día de vida. Se detectaron anticuerpos IgG contra Toxoplasma gondii en el 40,79% (93/228) de las muestras; de ellos el 23,6% (22/93) presentaban títulos elevados de anticuerpos IgG y de ellos se recogieron otras dos muestras de sangre periférica; se detectó una disminución de estos títulos. Conclusión: Los hallazgos muestran la importancia de fortalecer las acciones en la atención primaria de salud para prevenir la infección y capacitar a los profesionales de la salud en esta área para dotarlos de información sobre los casos de reinfección y reactivación de la infección en mujeres embarazadas, minimizando los riesgos para los bebés. Objetivo Jataí Goiás Método Resultados 22 vida 4079 40 79 40,79 93/228 93228 93 (93/228 236 23 6 23,6 22/93 2293 (22/93 periférica Conclusión embarazadas bebés 2 407 4 7 40,7 93/22 9322 9 (93/22 23, 22/9 229 (22/9 40, 93/2 932 (93/2 22/ (22/ 93/ (93/ (22 (93 (2 (9 (

ABSTRACT Purpose: This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich’s experimental tumor (EET) in mice. Methods: Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 106 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax. Results: The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract. Conclusions: The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells. Purpose L (EEOb Ehrlichs s (EET Methods control treated 6. 6 . 6) 21 10 0.05 005 0 05 (0.0 mL development days AgNOR (AgNOR antigen Bcl2 Bcl Bcl- Bax Results mgkg mg kg Control 37 3 7 3. 01 1 0. vs 57 5 5. 02 Conclusions 0.0 00 (0. (0 (

Abstract Background Computerized cognitive training programs may have benefited the self-assessment of memory, quality of life, and mood among older adults during the coronavirus disease 2019 (COVID-19) pandemic. Objective To determine the subjective impacts of computerized cognitive training on mood, frequency of forgetfulness, memory complaints, and quality of life in the elderly using an online platform. Methods In total, 66 elderly participants of USP 60 +, a program for the elderly offered by Universidade de São Paulo, who voluntarily enrolled in the study were selected and randomized with an allocation ratio of 1:1 into 2 groups: the training group (n = 33) and the control group (n = 33). After signing the free and informed consent form, they answered a protocol which included a sociodemographic questionnaire, the Memory Complaints Questionnaire (MAC-Q), the McNair and Kahn’s Frequency of Forgetfulness Scale, the Geriatric Depression Scale (GDS-15), the Geriatric Anxiety Inventory (GAI), and the Control, Autonomy, Self-Realization, and Pleasure (CASP-19) questionnaire. The training cognitive game platform aimed to stimulate various cognitive aspects, including memory, attention, language, executive functions (reasoning, logical thinking), and visual and spatial skills. Results The participants of the training group showed a reduction in the MAC-Q, MacNair and Kahn, and GAI scores in the pre- and posttest comparison. Significant differences were identified between the groups regarding the total scores of the MAC-Q in the post-test, which was also evidenced by the logistic regression. Conclusion Participation in a computerized cognitive intervention promoted reductions in memory complaints, frequency of forgetfulness, and anxiety symptoms, in addition to improving self-reported quality of life. selfassessment self assessment 201 COVID19 COVID 19 (COVID-19 pandemic forgetfulness complaints 6 + Paulo 11 1 1: n 33 33. . form questionnaire MACQ, MACQ MAC Q , (MAC-Q) Kahns Kahn s GDS15, GDS15 GDS 15 (GDS-15) GAI, (GAI) Control Autonomy SelfRealization, SelfRealization Self Realization, Realization Self-Realization CASP19 CASP (CASP-19 aspects attention language reasoning, reasoning (reasoning thinking, thinking thinking) skills Q, pre comparison posttest, post test, test post-test regression symptoms selfreported reported 20 COVID1 (COVID-1 3 (MAC-Q GDS1 (GDS-15 (GAI CASP1 (CASP-1 (COVID- (GDS-1 (CASP- (COVID (GDS- (CASP (GDS

Resumo Antecedentes Programas informatizados de treinamento cognitivo podem ter beneficiado a autoavaliação da memória, a qualidade de vida e o humor entre os idosos durante a pandemia de doença do coronavírus 2019 (coronavirus disease 2019, COVID-19, em inglês). Objetivo Determinar os impactos subjetivos do treinamento cognitivo computadorizado no humor, na frequência de esquecimento, nas queixas de memória, e na qualidade de vida em idosos utilizando uma plataforma online. Métodos Ao todo, 66 idosos participantes do programa USP 60 +, oferecido à terceira idade pela Universidade de São Paulo, e inscritos voluntariamente no estudo, foram selecionados e randomizados em uma razão de 1:1 em 2 grupos: grupo treinamento (n = 33) e grupo controle (n = 33). Após assinarem o termo de consentimento livre e esclarecido, os participantes responderam a um protocolo que incluía um questionário sociodemográfico, o Questionário de Queixas de Memória (Memory Complaints Questionnaire, MAC-Q), A Escala de Frequência de Esquecimento de McNair e Kahn, a Escala de Depressão Geriátrica (Geriatric Depression Scale, GDS-15), o Inventário de Ansiedade Geriátrica (Geriatric Anxiety Inventory, GAI), e –o questionário de Controle, Autonomia, Autorrealização e Prazer (Control, Autonomy, Self-Realization, and Pleasure, CASP-19). A plataforma de jogos cognitivos de treinamento visou estimular diversos aspectos cognitivos, incluindo memória, atenção, linguagem, funções executivas (raciocínio, raciocínio lógico) e habilidades visuais e espaciais. Resultados Na comparação pré e pós-teste, os participantes do grupo de treinamento apresentaram redução nas pontuações do MAC-Q, da escala McNair e Kahn e do GAI. Diferenças significativas entre os grupos quanto às pontuações totais da escala MAC-Q no pós-teste também foram evidenciadas pela regressão logística. Conclusão A participação em uma intervenção cognitiva computadorizada promoveu reduções nas queixas de memória, frequência de esquecimento e sintomas de ansiedade, além de melhorar a qualidade de vida autorrelatada. memória 201 coronavirus COVID19, COVID19 COVID 19, 19 COVID-19 inglês. inglês . inglês) online todo 6 + Paulo estudo 11 1 1: n 33 33. esclarecido sociodemográfico Memory Questionnaire MACQ, MACQ MAC Q , MAC-Q) Geriatric Scale GDS15, GDS15 GDS 15 GDS-15) Inventory GAI, GAI GAI) Controle Autonomia Control, Control (Control Autonomy SelfRealization, SelfRealization Self Realization, Realization Self-Realization Pleasure CASP19. CASP19 CASP CASP-19) atenção linguagem raciocínio, (raciocínio lógico espaciais pósteste, pósteste pós teste, teste Q, logística ansiedade autorrelatada 20 COVID1 COVID-1 3 GDS1 GDS-15 CASP1 CASP-19 COVID- GDS-1 CASP-1 GDS- CASP-

Low-density dispersive liquid-liquid microextraction with a subsequent esterification step was proposed in this study for the determination of linoleic acid and stearic acid in sheep blood serum samples. The method developed aimed to quickly and efficiently extract and esterify both fatty acids with subsequent analysis by gas chromatography/flame ionization detection. The extraction method was optimized with Scheffé’s polynomial model for three-component mixtures. The method was validated according to the parameters established by Eurachem Guide (2014). The optimal extraction conditions for LD-DLLME were 1400 µL of dispersion medium (MgCl2 0.017%), 400 µL of extractor solvent (toluene) and 1200 µL of dispersion solvent (methanol). The method performance showed adequate selectivity, sensitivity and precision to be applied to real samples, with an average recovery of 98.54% for linoleic acid and 103.83% for stearic acid. LD-DLLME was superior to the traditional method of analysis, which has been used until now for the determination of fatty acids in blood serum samples from ruminants. The analysis of real samples showed that the developed method is efficient for monitoring these substances in ruminants. Lowdensity Low density liquidliquid liquid chromatographyflame chromatography flame detection Scheffés Scheffé s threecomponent three component mixtures 2014. 2014 . (2014) LDDLLME LD DLLME 140 MgCl2 MgCl (MgCl 0.017%, 0017 0.017% , 0 017 0.017%) 40 toluene (toluene 120 methanol. methanol (methanol) selectivity 9854 98 54 98.54 10383 103 83 103.83 ruminants 201 (2014 14 001 0.017 01 4 12 (methanol 985 9 5 98.5 1038 10 8 103.8 20 (201 1 00 0.01 98. 103. 2 (20 0.0 (2 0. (

ABSTRACT Plant-growth-promoting bacteria of the genus Azospirillum and Herbaspirillum can improve crop yields of maize in tropical environments. The main mechanism proposed for its promotion is related to the auxins produced by these species. The aim of this study was to compare the maize growth response of inoculation using two species, Azospirillum baldaniorum Sp245 (Ab-Sp245) and Herbaspirillum seropedicae ZAE94 (Hs-ZAE94) during the initial growth under controlled conditions. A pot experiment was conducted with five harvests measuring bacterial counts, root morphology, biomass, and nitrogen content, and soluble metabolites for 38 days. Depending on the harvest period, a differential growth response between the two strains test was compared to the control. Plants use the bacterium applied to improve nitrogen acquisition and transform it into higher biomass and nitrogen accumulation, especially for Ab-Sp245. On the other side, Hs-ZAE94 altered the soluble metabolites resulting in higher NO3-, NH4+, and N-amino and lower soluble fractions compared to Ab-Sp245 and the control. The observed plant-bacteria interaction is closely fine-tuned and regulated by the strain used and differences in growth promotion effects cannot solely attributed to the root morphology. Plantgrowthpromoting Plant promoting environments species Sp Sp24 AbSp245 AbSp Ab (Ab-Sp245 ZAE ZAE9 HsZAE94 HsZAE Hs (Hs-ZAE94 conditions counts morphology content 3 days period control accumulation AbSp245. Sp245. side Hs-ZAE9 NO3, NO3 NO , NO3- NH4 NH NH4+ Namino N amino Ab-Sp24 plantbacteria plant finetuned fine tuned Sp2 AbSp24 (Ab-Sp24 HsZAE9 (Hs-ZAE9 Hs-ZAE Ab-Sp2 AbSp2 (Ab-Sp2 (Hs-ZAE Ab-Sp (Ab-Sp

Abstract The objective of this work was to evaluate inoculation response to Azospirillum baldaniorum strain Sp245 in 14 Urochloa cultivars. The evaluated cultivars were: Marandú, Xaraés, Paiaguás, Piatã, Basilisk, B140, Comum, Tupi, H47, Llanero, Ruziziensis, Ipyporã, Mulato, and Convert HD364. Three experiments were conducted to measure root traits: two using soil and one a sterile substrate. Compared with the control, a single application of A. baldaniorum Sp245 increases dry matter accumulation and number of roots in cultivars Marandú, Llanero, Ruziziensis, and Convert HD364. However, after inoculation, cultivars Xaraés and Ipyporã show some root traits with lower values. Sp Sp24 1 Marandú Paiaguás Piatã Basilisk B140 B Comum Tupi H47 H Llanero Ruziziensis Mulato HD364 HD substrate control A However values Sp2 B14 H4 HD36 B1 HD3

Resumo O objetivo deste trabalho foi avaliar a resposta à inoculação da estirpe Sp245 de Azospirillum baldaniorum em 14 cultivares de Urochloa. As cultivares avaliadas foram: Marandú, Xaraés, Paiaguás, Piatã, Basilisk, B140, Comum, Tupi, H47, Llanero, Ruziziensis, Ipyporã, Mulato e Convert HD364. Foram conduzidos três experimentos para mensuração de parâmetros radiculares: dois em solo e um em substrato estéril. Em comparação ao controle, a aplicação única de A. baldaniorum Sp245 aumenta o acúmulo de massa seca e a quantidade de raízes das cultivares Marandú, Llanero, Ruziziensis e Convert HD364. No entanto, após a inoculação, as cultivares Xaraés e Ipyporã apresentam alguns parâmetros radiculares com valores menores. Sp Sp24 1 Urochloa foram Marandú Paiaguás Piatã Basilisk B140 B Comum Tupi H47 H Llanero HD364 HD estéril controle A entanto menores Sp2 B14 H4 HD36 B1 HD3

ABSTRACT Inoculating corn with diazotrophic bacteria as growth promoters has been demonstrated to be an efficient agricultural practice in Brazil, mainly due to the root stimulation they provide to plants. This study investigates the corn (Zea mays L.) root architecture in a greenhouse assay where A. baldaniorum Sp245 and H. seropedicae ZAE94 strains were inoculated and evaluated for 22 days under two N levels: 0.6 and 6 mmol L-1 of N. Short-term bioassays were conducted to assess the plant’s response to the addition of indole-acetic acid, two bacterial populations, and two corn genotypes, utilizing image capture software WinRhizo Pro. The growth and distribution of tips, crossing, and length of fine roots were determined to be the most sensitive aspects to inoculation and indole-acetic acid induction. These responses were influenced by the genotype and the number of bacterial cells present. Biomass accumulation analyses quantified these modifications after a 22-day period. Additionally, the growth response was found to be more significant when applying the Hs-ZAE94 strain to plants fertilized with a higher dose of nitrogen (6.0 mmol L-1), and this response was positively correlated with bioassay data. Selected strains used as an inoculant can improve root architecture and, consequently, the N use efficiency. Brazil Zea L. L A Sp Sp24 H ZAE ZAE9 2 levels 06 0 0. L1 1 L- Shortterm Short term plant s indoleacetic indole acetic populations genotypes Pro tips crossing induction present 22day day period Additionally HsZAE94 HsZAE Hs Hs-ZAE9 6.0 60 (6. L1, , L-1) data consequently efficiency Sp2 HsZAE9 Hs-ZAE 6. (6 (

COVID-19 is currently the most critical disease in the world. Thus, several tools have been used in the last two years to contain the spread of SARS-CoV-2. In this perspective, Wastewater-Based Epidemiology (WBE) provides valuable data by including symptomatic, pre-symptomatic, and asymptomatic individuals and anticipating clinical cases. For 37 weeks, the SARS-CoV-2 viral load was monitored in sanitary sewage samples from Goiânia-Brazil. Through pre-concentration, extraction, and purification of viral RNA, followed by RT-qPCR, it was detected in 83.78% (31/37) of the weeks the presence of fragments of the genetic material SARS-CoV-2 with viral loads ranging from 105 to 108 genome copies L-1. The viral load in the wastewater samples was related to the clinical data of the municipality, demonstrating its ability to promote an Early Warning System (EWS). In early 2022, it was possible to predict the increase in clinical cases 3 weeks in advance and issue an alert note to the health authorities. Prevalence estimates ranged from 0.003 to 0.5% and showed good agreement with observed values. Thus, monitoring effluents become another essential tool that can help combat the pandemic’s advance, especially in countries with scarce resources. COVID19 COVID 19 COVID-1 world Thus SARSCoV2. SARSCoV2 SARSCoV SARS CoV 2. 2 perspective WastewaterBased Wastewater Based WBE (WBE symptomatic presymptomatic, presymptomatic pre pre-symptomatic SARS-CoV- GoiâniaBrazil. GoiâniaBrazil Goiânia Brazil. Brazil Goiânia-Brazil preconcentration, preconcentration concentration, concentration pre-concentration extraction RNA RTqPCR, RTqPCR RT qPCR, qPCR RT-qPCR 8378 83 78 83.78 31/37 3137 31 (31/37 10 L1. L1 L 1. 1 L-1 municipality EWS. EWS . (EWS) 2022 authorities 0003 0 003 0.00 05 5 0.5 values pandemics pandemic s resources COVID1 COVID- SARS-CoV 837 8 7 83.7 31/3 313 (31/3 L- (EWS 202 000 00 0.0 0. 83. 31/ (31/ 20 (31 (3 (

Background: Among chronic noncommunicable diseases (NCDs), Cardiovascular Diseases (CVD) are the main causes of premature mortality globally. The comprehensive care model focused on these diseases, presents as one of its components, the global cardiovascular risk screening (CVR).Objectives: To estimate cvR stratified by sociodemographic variables, as well as factors associated with moderate/high risk, in the adult population living in the municipality of Senador Canedo, metropolitan region of the state of Goiás, Midwest region of Brazil.Methods: The study was conducted through a household survey, through the application of a questionnaire with questions related to lifestyle and data collection such as weight, height, waist circumference, blood pressure, glycated hemoglobin and cholesterol dosage of 526 participants. Bivariate and multivariate analyses were performed using the Poisson regression model to analyze the factors associated with CVR according to the model proposed by the Framingham study.Results: The prevalence of High CVR was 12.2% (95%CI:9.5 - 15.5) and moderate CVR was 13.3% (95%CI:10.5 -16.8). The factors associated with high/moderate CVR were individuals without incomplete education or elementary (RPaj: 6.2; 95% CI: 1.3 - 29.7), insufficiently active (RPaj: 3.1; 95% CI: 1.8-5.0), and self-assessment of regular health status (RPaj: 1.8; 95% CI: 1.1-3.2).Conclusion: The present study allowed verifying the magnitude of CVR and the factors associated with high risk, consisting of an important instrument to guide the actions to prevent cardiovascular outcomes in the population attached to the family health strategy in the municipality of Senador Canedo.

Fundamentos: Dentre as Doenças Crônicas não Transmissíveis (DCNT), as Doenças Cardiovasculares (DCV) são as principais causas de mortalidade prematura globalmente. O modelo de atenção integral voltado para essas doenças, apresenta como um dos seus componentes, o rastreamento de risco global cardiovascular (RCV).Objetivos: Estimar o RCV estratificado por variáveis sociodemográficas, bem como fatores associados ao risco moderado/alto, na população adulta residente no município de Senador Canedo, região metropolitana da capital do estado de Goiás, Região Centro-Oeste do Brasil.Métodos: O estudo foi realizado por meio de um inquérito domiciliar, através da aplicação de questionário com perguntas relativas ao estilo de vida e coleta de dados como peso, altura, circunferência da cintura, pressão arterial, dosagem de hemoglobina glicada e de colesterol de 526 participantes. As análises bivariada e multivariada foram realizadas por meio do modelo de regressão de Poisson para analisar os fatores associados ao RCV segundo o modelo proposto pelo estudo de Framingham.Resultados: A prevalência do RCV Alto foi de 12,2% (IC95%:9,5 - 15,5) e do RCV Moderado foi 13,3% (IC95%:10,5 -16,8). Os fatores associados ao RCV Alto/moderado, foram: indivíduos sem instrução ou fundamental incompleto (RPaj: 6.2; IC 95%: 1.3 - 29.7), insuficientemente ativo (RPaj: 3.1; IC 95%: 1.8-5.0), e autoavaliação do estado de saúde regular (RPaj: 1.8; IC 95%: 1.1-3.2).Conclusão: O presente trabalho permitiu verificar a magnitude do RCV e os fatores associados ao alto risco, consistindo num importante instrumento para orientar as ações de prevenção de desfechos cardiovasculares na população adstrita a estratégia saúde da família no município de Senador Canedo.

ABSTRACT Purpose: To evaluate the effect of physical exercise on the behavior of rodents with colorectal cancer induced through the use of elevated plus maze. Methods: We used 40 male hairless mice induced to colorectal cancer, divided into five groups: G1) submitted to pre- and post-induction swimming; G2) pre- and post-induction ladder; G3) post-induction swimming; G4) post-induction ladder; G5) sedentary. At the end of the 14th week, the animals were submitted to the plus maze test. Results: The mean length of stay in the open arm for G1 was 4.17 ± 6.50; G2 37.52 ± 40.7; G3 85.84 ± 42.5; G4 32.92 ± 23.17; and G5 4.09 ± 4.43. In the closed arm, it was 264 ± 23.43 in G1, 187.60 ± 47.73 in G2, 147.50 ± 40.03 in G3, 182.00 ± 40.40 in G4, and in G5 235.36 ± 14.28. In the center, G1 remained 31.86 ± 20.18, G2 74.85 ± 28.37, G3 66.69 ± 19.53, G4 60.55 ± 10.46, and G5 60.55 ± 23.65. Conclusions: Aerobic exercise for seven weeks after tumor induction showed less impact on the behavior of the animals. On the other hand, it significantly increased the animals’ stress level when applied for 14 weeks before and after tumor induction. Purpose Methods 4 groups G pre postinduction post swimming ladder sedentary th week test Results 417 17 4.1 6.50 650 6 50 3752 37 52 37.5 40.7 407 7 8584 85 84 85.8 42.5 425 42 5 3292 32 92 32.9 23.17 2317 23 409 09 4.0 443 43 4.43 26 2343 23.4 18760 187 60 187.6 4773 47 73 47.7 14750 147 147.5 4003 03 40.0 18200 182 00 182.0 4040 40.4 23536 235 36 235.3 1428 28 14.28 center 3186 31 86 31.8 2018 20 18 20.18 7485 74 74.8 2837 28.37 6669 66 69 66.6 1953 19 53 19.53 6055 55 60.5 1046 10 46 10.46 2365 65 23.65 Conclusions hand 1 41 4. 6.5 375 3 37. 40. 858 8 85. 42. 329 9 32. 23.1 231 2 0 44 4.4 234 23. 1876 187. 477 47. 1475 147. 400 1820 182. 404 2353 235. 142 14.2 318 31. 201 20.1 748 74. 283 28.3 666 66. 195 19.5 605 60. 104 10.4 236 23.6 6. 14. 20. 28. 19. 10.