BACKGROUND Recurrence is a hallmark of ocular toxoplasmosis (OT), and conditions that influence its occurrence remain a challenge. Natural killer cells (NK) are effectors cells whose primary is cytotoxic function against many parasites, including Toxoplasma gondii. Among the NK cell receptors, immunoglobulin-like receptors (KIR) deserve attention due to their high polymorphism. OBJECTIVES This study aimed to analyse the influence of KIR gene polymorphism in the course of OT infection and its association with recurrences after an active episode. METHODS Ninety-six patients from the Ophthalmologic Clinic of the National Institute of Infectology Evandro Chagas were followed for up to five years. After DNA extraction, genotyping of the patients was performed by polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) utilising Luminex equipment for reading. During follow-up, 60.4% had a recurrence. FINDINGS We identified 25 KIR genotypes and found a higher frequency of genotype 1 (31.7%) with worldwide distribution. We note that the KIR2DL2 inhibitor gene and the gene activator KIR2DS2 were more frequent in patients without recurrence. Additionally, we observed that individuals who carry these genes progressed recurrence episodes slowly compared to individuals who do not carry these genes. MAIN CONCLUSIONS The KIR2DL2 and KIR2DS2 are associated as possible protection markers against ocular toxoplasmosis recurrence (OTR).

RESUMO Práticas estéticas e corporais têm ocupado um lugar significativo na atenção psicossocial, no contexto da Reforma Psiquiátrica brasileira. Propostas que inventam outras formas de produção de saúde e de subjetividade, associadas à participação social, têm valorizado a diversidade de formas de expressão em saúde mental e a diferença nas formas de existência. Com o intuito de estudar essas práticas e criar um campo de comunicação e produção coletiva de suas singularidades e seu plano comum, um grupo de pesquisadores se reuniu no Programa de Pós-graduação em Psicologia da Universidade Estadual Paulista e desenvolveu pesquisas articuladas ao projeto ‘Práticas estéticas e corporais na atenção psicossocial: processos de criação e produção de subjetividade’. Nessas pesquisas, foram exploradas as relações entre arte e saúde mental, a produção das políticas públicas relacionadas ao campo, o desenvolvimento das práticas clínicas, artísticas e culturais e a formulação de conceitos e perspectivas teóricas que sustentem essas práticas. Neste texto, serão apresentadas, brevemente, as políticas públicas de saúde e cultura que foram desenvolvidas no Brasil nos últimos 30 anos, sua articulação intersetorial e algumas experiências práticas nesse campo, que temos desenvolvido e pesquisado.

ABSTRACT Aesthetic and body practices have played a significant role in psychosocial care, in the context of the Brazilian Psychiatric Reform. Proposals that invent other ways of producing health and subjectivity, associated with social participation, have valued the diversity of expression in mental health and the difference in forms of existence. In order to study these practices and create a field of communication and collective production of their singularities and their common plan, a group of researchers met in the Graduate Program in Psychology at Universidade Estadual Paulista, creating the line of research ‘Aesthetic and body practices in the psychosocial care: processes of creation and production of subjectivity’. In the inquiries developed, the relations between art and mental health, the production of public policies related to the field, the development of clinical, artistic and cultural practices and the formulation of concepts and theoretical perspectives that support these practices were explored. In this paper, the health and culture public policies that have been developed in Brazil in the last 30 years, their intersectoral articulation and some practical experiences in this field, which we have developed and researched, will be briefly presented.

RESUMO Práticas estéticas e corporais têm ocupado um lugar significativo na atenção psicossocial, no contexto da Reforma Psiquiátrica brasileira. Propostas que inventam outras formas de produção de saúde e de subjetividade, associadas à participação social, têm valorizado a diversidade de formas de expressão em saúde mental e a diferença nas formas de existência. Com o intuito de estudar essas práticas e criar um campo de comunicação e produção coletiva de suas singularidades e seu plano comum, um grupo de pesquisadores se reuniu no Programa de Pós-graduação em Psicologia da Universidade Estadual Paulista e desenvolveu pesquisas articuladas ao projeto ‘Práticas estéticas e corporais na atenção psicossocial: processos de criação e produção de subjetividade’. Nessas pesquisas, foram exploradas as relações entre arte e saúde mental, a produção das políticas públicas relacionadas ao campo, o desenvolvimento das práticas clínicas, artísticas e culturais e a formulação de conceitos e perspectivas teóricas que sustentem essas práticas. Neste texto, serão apresentadas, brevemente, as políticas públicas de saúde e cultura que foram desenvolvidas no Brasil nos últimos 30 anos, sua articulação intersetorial e algumas experiências práticas nesse campo, que temos desenvolvido e pesquisado.

ABSTRACT Aesthetic and body practices have played a significant role in psychosocial care, in the context of the Brazilian Psychiatric Reform. Proposals that invent other ways of producing health and subjectivity, associated with social participation, have valued the diversity of expression in mental health and the difference in forms of existence. In order to study these practices and create a field of communication and collective production of their singularities and their common plan, a group of researchers met in the Graduate Program in Psychology at Universidade Estadual Paulista, creating the line of research ‘Aesthetic and body practices in the psychosocial care: processes of creation and production of subjectivity’. In the inquiries developed, the relations between art and mental health, the production of public policies related to the field, the development of clinical, artistic and cultural practices and the formulation of concepts and theoretical perspectives that support these practices were explored. In this paper, the health and culture public policies that have been developed in Brazil in the last 30 years, their intersectoral articulation and some practical experiences in this field, which we have developed and researched, will be briefly presented.

Neste relato de experiência apontamos a polifonia produzida com a escrita de diários de campo na formação do profissional da saúde. Com base no trabalho de campo vivido por professores e estudantes da Universidade Federal de São Paulo, no campus Baixada Santista (Unifesp-BS), São Paulo - Brasil, destacamos a importância da escrita sensível na elaboração das experiências vividas e as possibilidades que esta assume na formação. Os encontros aconteceram semanalmente durante um semestre no módulo - Eixo de Trabalho em Saúde, que integra o projeto político-pedagógico do campus na experiência interdisciplinar. A partir do método cartográfico e uma escrita a muitas mãos, o relato mistura reflexões teóricas e trechos de diários, criando um mapa de afetos sobre a experiência vivida, dando visibilidade a pontos de intensidades que se desdobram em reflexões para as áreas da saúde, artes e educação.

In this experience report, we pointed out the polyphony produced with the writing of field diaries in the health professional education. Based on the fieldwork of professors and students of the Federal University of São Paulo, at campus Baixada Santista (Unifesp-BS), we highlight the importance of a sensitive writing in the lived experiences elaboration process and the possibilities that it assumes in the formation process. The meetings took place weekly during one semester in the module – Axis: Work in Health, which integrates the campus’s pedagogical political project in the interdisciplinary experience. Using the cartographic method and a writing from very hands, the experience report mixes theoretical reflections and excerpts from diaries, creating a map of affections about the experience, giving visibility to points of intensity that unfold in reflections for health, arts and, education.

En este relato de experiencia señalamos la polifonía producida con la escritura de diarios de campo en la formación del profesional de la salud. A partir del trabajo de campo vivido por profesores y estudiantes de la Universidad Federal de São Paulo, en el campus Baixada Santista (Unifesp-BS), São Paulo - Brasil, subrayamos la importancia de la escritura sensible en la elaboración de las experiencias vividas y las posibilidades que ella asume en la formación. Los encuentros se realizaron semanalmente durante un semestre en el módulo Eje del Trabajo en Salud que integra el proyecto político-pedagógico del campus en la experiencia interdisciplinaria. A partir del método cartográfico y de una escritura a muchas manos, el relato mezcla reflexiones teóricas y trechos de diarios, creando un mapa de afectos sobre la experiencia vivida, dando visibilidad a puntos de intensidades que se desdoblan en reflexiones para las áreas de salud, artes y educación.

Neste relato de experiência apontamos a polifonia produzida com a escrita de diários de campo na formação do profissional da saúde. Com base no trabalho de campo vivido por professores e estudantes da Universidade Federal de São Paulo, no campus Baixada Santista (Unifesp-BS), São Paulo - Brasil, destacamos a importância da escrita sensível na elaboração das experiências vividas e as possibilidades que esta assume na formação. Os encontros aconteceram semanalmente durante um semestre no módulo - Eixo de Trabalho em Saúde, que integra o projeto político-pedagógico do campus na experiência interdisciplinar. A partir do método cartográfico e uma escrita a muitas mãos, o relato mistura reflexões teóricas e trechos de diários, criando um mapa de afetos sobre a experiência vivida, dando visibilidade a pontos de intensidades que se desdobram em reflexões para as áreas da saúde, artes e educação.

In this experience report, we pointed out the polyphony produced with the writing of field diaries in the health professional education. Based on the fieldwork of professors and students of the Federal University of São Paulo, at campus Baixada Santista (Unifesp-BS), we highlight the importance of a sensitive writing in the lived experiences elaboration process and the possibilities that it assumes in the formation process. The meetings took place weekly during one semester in the module – Axis: Work in Health, which integrates the campus’s pedagogical political project in the interdisciplinary experience. Using the cartographic method and a writing from very hands, the experience report mixes theoretical reflections and excerpts from diaries, creating a map of affections about the experience, giving visibility to points of intensity that unfold in reflections for health, arts and, education.

En este relato de experiencia señalamos la polifonía producida con la escritura de diarios de campo en la formación del profesional de la salud. A partir del trabajo de campo vivido por profesores y estudiantes de la Universidad Federal de São Paulo, en el campus Baixada Santista (Unifesp-BS), São Paulo - Brasil, subrayamos la importancia de la escritura sensible en la elaboración de las experiencias vividas y las posibilidades que ella asume en la formación. Los encuentros se realizaron semanalmente durante un semestre en el módulo Eje del Trabajo en Salud que integra el proyecto político-pedagógico del campus en la experiencia interdisciplinaria. A partir del método cartográfico y de una escritura a muchas manos, el relato mezcla reflexiones teóricas y trechos de diarios, creando un mapa de afectos sobre la experiencia vivida, dando visibilidad a puntos de intensidades que se desdoblan en reflexiones para las áreas de salud, artes y educación.

One new methodology for analysis of the antimicrobial oxolinic acid (OXA) based on adsorptive stripping voltammetry (AdSV) in carbon paste electrode (CPE) was developed and applied in untreated pond water sample. Cyclic voltammetry (CV) indicated that an irreversible anodic process occurs in KOH solution as supporting electrolyte. Quantification of the antimicrobial was carried out by differential pulse adsorptive stripping voltammetry (DPAdSV) at potential of deposition (ED) +1.05 V for 45 s to examine OXA adsorption on the surface of CPE. The limits of detection (LOD) and quantification (LOQ) of the method for OXA analysis were determined. The values of LOD were 0.0570 and 0.512 mg L-1 in absence of matrix and in the water sample, respectively, and the corresponding values of LOQ were 0.192 and 1.707 mg L-1, respectively. The apparent recovery percentage for different concentrations of OXA in natural waters ranged between 98.4 and 109%.

This study evaluated the finishing and polishing effect on the surface roughness and hardness of the Filtek Supreme XT, in fluoride solutions. Specimens were prepared (n = 140) with half of the samples finished and polished with Super-Snap® disks. The experimental groups were divided according to the presence or absence of finishing and polishing and immersion solutions (artificial saliva, sodium fluoride solution at 0.05% - manipulated, Fluordent Reach, Oral B, Fluorgard). The specimens remained immersed in artificial saliva for 24 hours and were then subjected to initial analysis (baseline) of surface roughness and Vickers microhardness. Next, they were immersed in different fluoride solutions for 1 min/day, for 60 days. Afterwards, a new surface roughness and microhardness reading was conducted. The data were submitted to a two-way ANOVA and Tukey's test (5% significance level). For the comparison of mean roughness and hardness at baseline and after 60 days, the paired Student t test was used. The results showed that the surface roughness and microhardness of the Filtek Supreme XT were influenced by the finishing and polishing procedure, independently of the immersion methods.

This study evaluated the effect of surface sealant on the translucency of composite resin immersed in different solutions. The study involved the following materials: Charisma, Fortify and coffee, Coca-Cola®, tea and artificial saliva as solutions. Sixty-four specimens (n = 8) were manufactured and immersed in artificial saliva at 37 ± 1 °C. Samples were immersed in the solutions for three times a day and re-immersed in artificial saliva until the translucency readings. The measurements were carried out at nine times: T1 - 24 hours after specimen preparation, T2 - 24 hours after immersion in the solutions, T3 - 48 hours and T4 to T9 - 7, 14, 21, 30, 60 and 90 days, respectively, after immersion. The translucency values were measured using a JOUAN device. The results were subjected to ANOVA and Tukey's test at 5%. The surface sealant was not able to protect the composite resin against staining, the coffee showed the strongest staining action, followed by tea and regarding immersion time, a significant alteration was noted in the translucency of composite resin after 21 days.

O comércio de alimentos prontos para o consumo por vendedores ambulantes pode constituir um alto risco para a saúde dos consumidores, visto que as pessoas envolvidas nesta atividade geralmente não tem preparo para a manipulação correta de alimentos. Neste trabalho foram investigadas as condições de preparo e a qualidade higiênico-sanitária de lanches comercializados por vendedores ambulantes em Pelotas. Foram colhidas de 60 estabelecimentos, uma amostra de água, uma da superfície de manuseio e um lanche (cachorro-quente). Nos cachorros-quentes foram realizadas contagens de bactérias aeróbias mesófilas (BAM), Staphylococcus coagulase positiva (STA), coliformes totais (CT), coliformes a 45ºC (CF), e investigada a presença de Salmonella sp. Na água e superfície foram realizadas contagens de BAM, CT e CF. Entre as 60 amostras de cachorros-quentes analisadas, 53%, 48%, 37% e 25% estavam fora do padrão para CT, BAM, STA e CF, respectivamente. Em nenhuma amostra foi detectada a presença de Salmonella. As amostras de água apresentaram apenas 3 (5%) amostras fora do padrão para BAM, e 27% e 23% fora do padrão para CT e CF, respectivamente. Em relação às superfícies, 70% foram consideradas insatisfatórias para contagem de BAM e 68% e 67% para CT e CF. Os resultados sugerem que as condições higiênicas existentes em muitos estabelecimentos não são adequadas, o que se reflete em uma proporção relativamente alta de lanches com qualidade microbiológica insatisfatória para o consumo.

Street-vending of ready-to-eat (RTE) foods can be a risk to the consumers health, since people usually involved in this activity does not have proper knowledge in safe handling of foods. Despite this, only a few studies have been made on the microbiological quality of these foods and places where they are prepared. In this paper we report on the higienic-sanitary quality of hot-dogs sold by street-vendors from Pelotas,RS. Samples of hot-dogs, water and work surfaces, were collected from 60 street-vending places and taken to the laboratory for analysis. Counts of aerobic mesophilic bacteria (TPC), Staphylococcus coagulase positive (SCP), total coliforms (TC) and coliforms at 45ºC (FC) were made on samples of hot-dogs. SPC, TC and FC counts were made on the water and surface samples. Among the 60 samples of hot-dogs 53%, 48%, 37% and 25% were found unsatisfactory for TC, TPC, STA and FC, respectively. Only 3 (5%) water samples were found unsatisfactory according to the TPC standard used, and 27% and 23% did not read the standards for TC and FC. Regarding the surfaces, 70% were found unsatisfactory for TPC, 68% for TC and 67% for FC. Salmonella sp was not detected in any of the samples tested. These results suggest that the hygiene practices of many food street-vending places are not adequate, resulting in a high proportion of read-to-eat RTE) foods with microbiological quality unsatisfactory for consumption.