Abstract This research aimed to evaluate the effect of the radiopacity of a Bulk-Fill composite (X-TraFil, VOCO, Germany) and a Conventional composite (P60, 3M ESPE, USA) and assessment of the margin location in the enamel and dentin on the diagnosis of secondary caries. 76 intact premolars with MOD preparation were divided into two equal groups and filled with the conventional and bulk-fill composite. Four regions were considered to simulate carious lesions (two regions in enamel and two regions in dentin). In each group, half of the regions in the dentin and half in the enamel were randomly selected for secondary caries simulation and filled with a wax-plaster combination while the remaining regions stayed intact. Bitewing imaging was done using the PSP digital sensor. Five examiners reviewed the images, and lesions were recorded. Caries diagnosis indicators and paired-sample t-test were used for statistical analysis. The reproducibility and accuracy of the examiners’ responses were evaluated using the kappa and agreement coefficient (α=0.05). The sensitivity, specificity, and accuracy of diagnosing secondary carious lesions in enamel were significantly better under conventional than bulk-fill composite. Similarly, the sensitivity and accuracy of diagnosing secondary caries in dentin were significantly higher under conventional composite than bulk-fill composite (p<0.05). No significant differences were found in the agreement and kappa coefficient between conventional and bulk-fill composites in the enamel and dentin (p>0.05). The diagnostic accuracy of carious lesions was higher under conventional composite than bulk-fill composite. However, the location of the secondary was ineffective in caries diagnosis. BulkFill Bulk Fill XTraFil, XTraFil X TraFil, TraFil (X-TraFil VOCO Germany P60, P60 P (P60 M ESPE USA 7 bulkfill bulk fill dentin. . dentin) group waxplaster wax plaster sensor images recorded pairedsample paired sample ttest t test analysis α=0.05. α005 α α=0.05 0 05 (α=0.05) specificity Similarly p<0.05. p005 p p<0.05 (p<0.05) p>0.05. p>0.05 (p>0.05) However P6 (P6 α00 α=0.0 (α=0.05 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 (P α0 α=0. (α=0.0 p0 p<0. (p<0.0 p>0. (p>0.0 α=0 (α=0. p<0 (p<0. p>0 (p>0. α= (α=0 p< (p<0 p> (p>0 (α= (p< (p> (α (p

Resumo Essa pesquisa teve como objetivo avaliar o efeito da radiopacidade de resina composta Bulk-Fill (X-TraFil, VOCO, Alemanha) e de resina composta convencional (P60, 3M ESPE, EUA) e a avaliação da localização da margem no esmalte e na dentina no diagnóstico de cárie secundária. 76 pré-molares intactos com preparo MOD foram divididos em dois grupos iguais e restaurados de acordo com o grupo experimental. Quatro regiões foram consideradas para simular lesões de cárie (duas regiões no esmalte e duas regiões na dentina). Em cada grupo, metade das regiões na dentina e metade no esmalte foram selecionadas aleatoriamente para a simulação de cárie secundária e preenchidas com uma combinação de cera e gesso, enquanto as regiões restantes permaneceram intactas. As imagens de bitewing foram feitas usando o sensor digital PSP. Cinco examinadores analisaram as imagens, e as lesões foram registradas. Os indicadores de diagnóstico de cárie e o teste t de amostra pareada foram usados para análise estatística. A reprodutibilidade e a precisão das respostas dos examinadores foram avaliadas usando o kappa e o coeficiente de concordância (α=0,05). A sensibilidade, a especificidade e a precisão do diagnóstico de lesões cariosas secundárias no esmalte foram significativamente melhores com a resina composta convencional do que com a resina composta bulk-fill. Da mesma forma, a sensibilidade e a precisão do diagnóstico de cáries secundárias na dentina foram significativamente maiores com a resina convencional do que com a resina bulk-fill (p<0,05). Não foram encontradas diferenças significativas na concordância e no coeficiente kappa entre as resinas convencionais e bulk-fill no esmalte e na dentina (p>0,05). A precisão do diagnóstico de lesões cariosas foi maior com a resina composta convencional do que com a resina composta bulk-fill. Entretanto, a localização do secundário foi ineficaz no diagnóstico de cárie. BulkFill Bulk Fill XTraFil, XTraFil X TraFil, TraFil (X-TraFil VOCO Alemanha P60, P60 P (P60 M ESPE EUA 7 prémolares pré molares experimental dentina. . dentina) gesso intactas PSP registradas estatística α=0,05. α005 α α=0,05 0 05 (α=0,05) bulkfill. bulkfill bulk fill. fill forma p<0,05. p005 p p<0,05 (p<0,05) p>0,05. p>0,05 (p>0,05) Entretanto P6 (P6 α00 α=0,0 (α=0,05 p00 p<0,0 (p<0,05 p>0,0 (p>0,05 (P α0 α=0, (α=0,0 p0 p<0, (p<0,0 p>0, (p>0,0 α=0 (α=0, p<0 (p<0, p>0 (p>0, α= (α=0 p< (p<0 p> (p>0 (α= (p< (p> (α (p

ABSTRACT Objective: To investigate the effects of two different dentifrice fluoride concentrations on the color stability of the composite. Material and Methods: Twenty-seven specimens (2×4×5 mm) each of microfilled (Gradia, GC, Japan) and nanohybrid (Grandio, VOCO, Germany) composites were prepared. The specimens were randomly divided into six groups (control, Fluoflor caries protection toothpaste with 1450ppm Fluoride (EXW, France), and Fluoflor kids toothpaste with 500ppm Fluoride (EXW, France) (n = 9). The specimens were immersed in a mixture of artificial saliva and toothpaste in a ratio of 1:3 and applied for 60 seconds every 12 hours for 42 days. The control samples were incubated in artificial saliva at 37°C. Primary and secondary color measurements were performed using color parameters (L∗a∗b) with a spectrophotoshade (MHT Optic Research AG, Niederhasli, Switzerland). Data were analyzed using a two-way analysis of variance at a significance level of 0.05. Results: According to the two-way ANOVA analysis, there was no significant difference in color change between the composites and no difference in the level of discoloration between different fluoride concentrations(p>0.05). Also, None of the dentifrices caused clinically significant color changes(∆E˂3.3). Conclusion: No clinically unacceptable color changes were observed in the microfilled and nanofilled composites with different concentrations of fluoride toothpaste. Objective composite Methods Twentyseven Twenty seven 2×4×5 245 2 4 5 (2×4× mm Gradia, Gradia (Gradia GC Japan Grandio, Grandio (Grandio VOCO Germany prepared control, (control ppm EXW, EXW (EXW France, France , n 9. 9 . 9) 13 1 3 1: 6 days 37C C 37 37°C L∗a∗b Lab L b (L∗a∗b MHT AG Niederhasli Switzerland. Switzerland Switzerland) twoway way 005 0 05 0.05 Results concentrationsp>0.05. concentrationsp005 concentrationsp p>0.05 p concentrations(p>0.05) Also changes∆E˂3.3. changesE33 changesE ∆E˂3.3 E changes(∆E˂3.3) Conclusion 2×4× 24 (2×4 00 0.0 concentrationsp>0.05 concentrationsp00 p005 p>0.0 concentrations(p>0.05 changes∆E˂3.3 changesE3 E33 ∆E˂3. changes(∆E˂3.3 2×4 (2× 0. concentrationsp>0.0 concentrationsp0 p00 p>0. concentrations(p>0.0 changes∆E˂3. E3 ∆E˂3 changes(∆E˂3. 2× (2 concentrationsp>0. p0 p>0 concentrations(p>0. changes∆E˂3 ∆E˂ changes(∆E˂3 ( concentrationsp>0 p> concentrations(p>0 changes∆E˂ ∆E changes(∆E˂ concentrationsp> concentrations(p> changes∆E changes(∆E concentrations(p

Abstract Objective: To compare the cytotoxicity level of a new calcium silicate-based resin cement (TheraCem) with two commonly used cements, including a conventional self-adhesive resin cement (Panavia SA) and a resinmodified glass ionomer cement (FujiCem2), on the human gingival fibroblast cells after 24 and 48 hours. Material and Methods: Twelve discs of each cement type were fabricated. The extract of cement disks was made by incubating them in the cell medium. Human gingival fibroblast cells were cultured and exposed to cement extracts for 24 h and 48 h. MTT assay was performed on extracts and optical density and cell viability rates were calculated by the spectrophotometer device at 570 nm. Data were analyzed using ANOVA and Tukey HSD tests. Results: The cell viability rates after 24 hours and 48 hours were as follows: TheraCem: 89.24% and 85.46%, Panavia SA: 49.51% and 46.57% and FujiCem2: 50.63% and 47.36%. TheraCem represented the highest cell viability rate. However, no significant difference was noted between Panavia SA and FujiCem2. Time had no significant effect on cell viability. Conclusion: TheraCem exhibited the best results among three tested cements and was considered non-toxic. Panavia SA and FujiCem2 were not significantly different regarding the cell viability rate. Time had no significant effect on the cytotoxicity level of cements.

Abstract This study considered a possible relationship between the severity of oral lichen planus (OLP), serum anti-TPO autoantibodies (TPOAb) titer and thyroid disease in OLP patients. Forty-six OLP patients with positive TPOAb results (> 35 IU/ml) who had also been diagnosed with thyroid disease were included in the study group. The control group consisted of 46OLP patients with no thyroid disease. The study and control groups (92) were divided to two subgroups of erosive OLP (EOLP) and non-erosive OLP (NEOLP). Serum TPOAb levels and IL-8 (to measure OLP severity) were evaluated using the independent t-test, chi-square and conditional logistic regression analysis (α = 0.05). A significant positive correlation was found between serum IL-8 and TPOAb levels in the study group (r = 0.783; p = 0.001). The positive blood levels of TPOAb were significantly associated with an increased risk of EOLP (OR = 4.02 at 95%CI; 1.21–13.4; p = 0.023). It is possible to used positive serum TPOAb levels in patients with OLP as in indicator of possible undetected thyroid disorders in those patients. Because erosive OLP has been associated with TPOAb in thyroid patients, it may be useful to determine TPOAb levels of such patients to diagnose a possible undetected thyroid disorders and follow-up for malignancy.

To reduce the dependence on the sometimes unwise use of synthetic pesticides in fruit and vegetable plantations, the toxicity and repellence of Peganum harmala L. (Zygophyllaceae) acetonic seed extract was assayed against several insect pests. For contact toxicity, 3- to 4-d-old individuals of Aphis fabae Scopoli, A. gossypii Glover, A. nerii Boyer de Fonscolombe, and Myzus persicae (Sulzer) were included, as well as 1- to 7-d-old adult Tribolium castaneum (Herbst). Repellent effect experiments were conducted on adult, 1- to 2- and 3- to 4-d old M.persicae individuals. At 60 mg mL4, the topical bioassay mortality percentage was significantly higher in A. gossypii than in A. fabae and A. nerii after 12-72 h. Mortality of the treatments on M.persicae was 87.1% and 90.0% after 24 and 48 h, respectively, and significantly higher than A. fabae and A. nerii during this period. At 60 mg mL-1, the mortality of T. castaneum was much lower than that of the aphid species. The highest repellent index (over 72%) was observed on 1- to 2-d-old M. persicae individuals.

Para reducir la dependencia de los pesticidas sintéticos en plantaciones frutales y hortalizas, se realizó un ensayo para medir la toxicidad y repelencia de un extracto acetónico obtenido a partir de semillas de Peganum harmala L. (Zygophyllaceae) contra diferentes especies de plagas. Para evaluar la toxicidad del extracto al contacto con los insectos, se incluyeron individuos de 3-4 d de edad de Aphis fabae Scopoli, Aphis gossypii Glover, Aphis nerii Boyer de Fonscolombe, y Myzus persicae (Sulzer), así como adultos 1-7 d de edad de Tribolium castaneum (Herbst). Experimentos para medir el efecto repelente se llevaron a cabo con individuos de 1-2 y 3-4 d de edad de M. persicae. En los resultados de los bioensayos tópicos el porcentaje de mortalidad fue significativamente mayor en la especie A. gossypii que en A. fabae y A. nerii, después de 12-72 h con una concentración de 60 mg mL-1. La mortalidad de los tratamientos contra M. persicae fueron 87,1% y 90,0% después de 24 y 48 h, respectivamente, la cual fue significativamente mayor que la obtenida con A. fabae y A. nerii durante el mismo período de tiempo. Con la concentración de 60 mg mL-1, la mortalidad de T. castaneum fue muy inferior a las obtenidas con las diferentes especies de áfidos. El mayor índice de repelencia (mayor a 72%) se observó en individuos de 1 a 2 d de edad de M. persicae entre los diferentes tratamientos.