Abstract Adipose derived mesenchymal stem cells (AMSCs) have been isolated from domestic and wild cats. For wild cats, the isolation of AMSCs has been reported in the black-footed cats (Felis nigripes) and guigna (Leopardus guigna). Stromal vascular fraction (SVF) isolated from cougar adipose tissue have been used to restore elbow functionality in the cougar (Puma concolor) but multipotent characteristics of these cells have not been described. The present study describes for the first time the isolation and characterization of mesenchymal stem cells derived from adipose tissue of cougar. AMSCs and fibroblasts from six months female cougar were isolated and cultured in DMEM/F12, supplemented with FBS 10% + 1% Antibiotic/Antifungal + 2.4 mM L-Glutamine + 2.4 mM pyruvate up to passage 5. Expression of pluripotent and surface marker genes was evaluated at mRNA level. Mesodermal differentiation (adipogenic, osteogenic and chondrogenic) was described. AMSCs expressed mRNA of pluripotent genes Oct4, Nanog, Sox2 and Klf4 and surface markers Cd44, Cd90, Cd105 and MHCII. Fibroblasts showed similar mRNA expression with the exception of Sox2. AMSCs obtained from cougar exhibit multipotency features similar to domestic cats MSC, nevertheless, other analyses are required. AMSCs from cougar could be a source of interest for treatment of individuals that remain in captivity or arrive to wildlife rehabilitation centers.

Abstract: The postmodern society based on a neoliberal model questions the strong value component characteristic of professional nursing care. Faced with this situation, the question arises: How is valuation training faced by nursing students and how are these students living in the practice of care? Objective: To analyze what has been researched in the last ten years about the ethical formation in nursing students and the value practice of these professionals in a postmodern society. Methodology: Mixed systematic review of a narrative nature that I consider the last ten years (2008-2018), includes 76 articles, of which 48 were selected for analysis. The databases were revised: ProQuest, ProQuest education Database, ProQuest Religion Database, Ebsco, Cuiden, Dialnet plus, Latindex, Scielo, SCOPUS and Google academic. Conclusions: Postmodernism upsets professional values, both in the practice of care and in the training of future professionals, for this reason it is necessary to strengthen and adapt the traditional values between the academy and the clinic, focusing the training on the teacher as a model of professional practice.

Resumen: La sociedad postmoderna basada en un modelo neoliberal cuestiona el fuerte componente valórico característico del cuidado profesional de enfermería. Ante esta situación surge la interrogante: ¿Cómo se enfrenta la formación valórica en los estudiantes de enfermería y cómo se viven estos en la práctica del cuidado? Objetivo: Analizar qué se ha investigado en los últimos diez años sobre la formación ética en estudiantes de enfermería y en la práctica valórica de estos profesionales en una sociedad postmoderna. Metodología: Revisión sistemática mixta de carácter narrativo que considero los últimos diez años (2008-2018), incluye 76 artículos de los cuales se seleccionaron 48 para su análisis. Se revisaron las bases de datos: ProQuest, ProQuest education Database, ProQuest Religión Database, Ebsco, Cuiden, Dialnet plus, Latindex, Scielo, SCOPUS y Google académico. Conclusiones: El postmodernismo trastoca los valores profesionales, tanto en la práctica del cuidado como en la formación de los futuros profesionales, por ello se requiere fortalecer y adecuar los valores tradicionales entre la academia y la clínica, centrando la formación en el profesor como modelo de la práctica profesional.

Resumo: A sociedade pósmoderna baseada em um modelo neoliberal questiona o forte componente de valor característico do cuidado profissional de enfermagem. Diante desta situação surge a pregunta: Como se enfrente a formação de valores nos estudantes de enfermagem e como isto é vivido na prática do cuidado? Objetivo: Analisar o que se investigou nos últimos dez anos sobre a formação ética nos estudantes de enfermagem e na prática de valor destes profissionais em uma sociedade pósmoderna. Metodologia: revisão sistemática mista de caráter narrativo que considere os últimos dez anos (2008-2018), incluindo 76 artigos dos quais se selecionaram 48 para análise. Foram revisadas as bases de dados: ProQuest, ProQuest education Database, ProQuest Religión Database, Ebsco, Cuiden, Dialnet plus, Latindex, Scielo, SCOPUS e Google acadêmico. Conclusões: O postmodernismo transtorna os valores profissionais, tanto na prática do cuidado como na formação dos futuros profissionais, por isso se requer fortalecer e adequar os valores tradicionais entre a academia e a clínica, centrando a formação no professor como modelo da prática profissional

Resumen: Introducción: El objetivo de este ensayo es reflexionar acerca del concepto de gestión en enfermería con el fin de visibilizar a países latinoamericanos que han incorporado la gestión del cuidado a sus normativas, en específico la experiencia chilena y los aspectos legales que rigen la gestión del cuidado. Desarrollo: Para conseguir dicho objetivo, se efectuó una revisión bibliográfica en diversas revistas de corriente principal, textos y leyes relacionadas con el gerenciamiento y la gestión del cuidado. Inicialmente, se analizó el concepto de gestión con la intención de comprender por qué la enfermería lo integra dentro de sus ámbitos de desempeño; luego, se buscó visibilizar cómo algunos países de Latinoamérica han incorporado, dentro de sus marcos legales, la gestión del cuidado a la profesión de enfermería, por último, se analiza la experiencia de la enfermería chilena al respecto. Conclusión: Como consideraciones finales se obtiene que la gestión del cuidado es parte de la función social de las enfermeras/os; por tanto, desempeñar el trabajo diario incorporando lo gerencial, otorgará competencias gestoras y facilitará el cuidado autónomo para tales profesionales.

Abstract: Introduction: The objective of this article is to reflect on the implication of the concept of management of nursing, making Latin-American countries that have incorporated the management of care aware of its norms and the Chilean experience and the legal aspects that regulate the management of care are specifically presented. Development: To achieve this objective, a bibliographic revision of diverse mainstream magazines, texts and laws related to administration and management of care was carried out. Initially, the concept of management is analyzed with the purpose of comprehending why the nursing integrates it in its field of performance. To visualize later how some of the countries of Latin America have incorporated it in their legal frameworks of management of care of nursing, and subsequently the experience of the Chilean nursing with regard to the management of care is analyzed extensively. Conclusions: As final considerations it is indicated that the management of care is part of the social function of the nurses, for the daily work they are to perform incorporating the management, it would provide managing competencies and would permit the autonomous caretaking to these professionals.

Background: Telithromycin is a new ketolide antimicrobial, that can be useful for the treatment of respiratory infections. Aim: To compare in vitro activity of telithromycin against respiratory pathogens, isolated in outpatient clinics. Material and methods: Two hundred eighty strains isolated from patients with respiratory infections, were studied. The strains studied were S pneumoniae, penicillin sensitive (SPNS:57); intermediate (SPNI:35), resistant (SPNR:25); S pyogenes (SP:57); H influenzae (HIN 51); M catarrhalis (MC:25) and S aureus meticillin sensitive (SAUS:30). Minimal inhibitory concentration (MIC) by broth microdilution was studied for telitrhomycin and levofloxacin in all strains. Other antimicrobials studied, but not in all strains were erythromycin, clindamycin, trimetoprim sulphamethoxazole, oxacillin, amoxicillin-clavulanic acid and cefuroxime. Results: All strains were sensible to telithromycin at a concentration 4 µg/ml. MIC 90 and its range for SPNS was 0.03 µg/ml (0.004-0.12), for SPNI was 0.03 µg/ml (0.004-025), for SPNR was 0.06 µg/ml (0.004-0.25), for HIN was 2 µg/ml (0.12-4), for SP was 0.5 µg/ml (0.004-2), for MC was 0.5 µg/ml (0.06-2) and for SAU was 0.25 µg/ml (0.06-0.25). Conclusions: All studied pathogens were sensible to telithromycin in vitro. This antimicrobial is an alternative for the treatment of community acquired respiratory infections