Abstract To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT). Methods: The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT. Saliva samples were collected as a source of genomic DNA. Genetic polymorphisms were genotyped by Real-Time PCR using the Taqman method. Univariate and Multivariate analyses were used (p<0.05). Results: A significant difference was observed for the polymorphism rs2297518 in the NOS2 gene in functional limitation in the codominant (p=0.037) and recessive (p=0.001) models; in the physical pain (p<0.001 in both models); in psychological discomfort (p<0.001 in both models); in physical disability (p<0.001 in both models) and in psychological disability (p<0.001 in both models). Polymorphisms in the SOCS1 gene, in the recessive model, rs33977706 (p=0.045) and rs243327 (p=0.019), influenced the OHRQoL in the psychological discomfort domain. Conclusions: Polymorphisms in NOS2 and SOCS1 genes influenced the OHRQoL of patients undergoing RCT. IL1A ILA IL rs17561 rs rs1304037 IL1 rs1800871 ILRN RN rs9005, rs9005 , rs9005) NOS (NOS rs2779249 rs2897518 SOCS (SOCS healthrelated health related (OHRQoL under going RCT . (RCT) Methods 10 participants singlerooted single rooted periodontitis OHIP14 OHIP 14 (OHIP-14 before seven 3 DNA RealTime Real Time method p<0.05. p005 p p<0.05 0 05 (p<0.05) Results rs229751 p=0.037 p0037 037 (p=0.037 p=0.001 p0001 001 (p=0.001 models p<0.001 (p<0.00 models. model rs3397770 p=0.045 p0045 045 (p=0.045 rs24332 p=0.019, p0019 p=0.019 019 (p=0.019) domain Conclusions rs1756 rs130403 rs180087 rs900 rs277924 rs289751 (RCT 1 OHIP1 (OHIP-1 p00 p<0.0 (p<0.05 rs22975 p=0.03 p003 03 (p=0.03 p=0.00 p000 00 (p=0.00 p<0.00 (p<0.0 rs339777 p=0.04 p004 04 (p=0.04 rs2433 p001 p=0.01 01 (p=0.019 rs175 rs13040 rs18008 rs90 rs27792 rs28975 (OHIP- p0 p<0. rs2297 p=0.0 (p=0.0 (p<0. rs33977 rs243 (p=0.01 rs17 rs1304 rs1800 rs9 rs2779 rs2897 (OHIP p<0 rs229 p=0. (p=0. (p<0 rs3397 rs24 rs1 rs130 rs180 rs277 rs289 p< rs22 p=0 (p=0 (p< rs339 rs2 rs13 rs18 rs27 rs28 p= (p= (p rs33 rs3

Resumo Avaliar o impacto de polimorfismos genéticos em interleucinas (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), óxido nítrico (NOS2 rs2779249, rs2897518) e supressor da sinalização de citocinas (SOCS1 rs243327, rs33977706) na qualidade de vida relacionada à saúde bucal (QVRSB) de pacientes submetidos a tratamento endodôntico (TE). Métodos: A amostra foi composta por 108 participantes, que apresentavam dentes unirradiculares com lesão periapical assintomática. O impacto da QVRSB foi registrado usando o Oral Health Impact Profile (OHIP-14) antes, sete e 30 dias após o TE. Amostras de saliva foram coletadas como fonte de DNA genômico. Os polimorfismos genéticos foram genotipados por PCR em tempo real usando o método Taqman. Análises univariadas e multivariadas foram utilizadas (p<0,05). Resultados: Observou-se diferença significativa para o polimorfismo rs2297518 no gene NOS2 na limitação funcional nos modelos codominante (p=0,037) e recessivo (p=0,001); na dor física (p<0,001 em ambos os modelos); no desconforto psicológico (p<0,001 em ambos os modelos); na deficiência física (p<0,001 em ambos os modelos) e na deficiência psicológica (p<0,001 em ambos os modelos). Polimorfismos no gene SOCS1, no modelo recessivo, rs33977706 (p=0,045) e rs243327 (p=0,019), influenciaram a QVRSB no domínio desconforto psicológico. Conclusões: Polimorfismos nos genes NOS2 e SOCS1 influenciaram a QVRSB de pacientes submetidos a TE. IL1A ILA IL rs17561 rs rs1304037 IL1 rs1800871 ILRN RN rs9005, rs9005 , rs9005) NOS (NOS rs2779249 rs2897518 SOCS (SOCS (QVRSB TE . (TE) Métodos 10 participantes assintomática OHIP14 OHIP 14 (OHIP-14 antes 3 genômico Taqman p<0,05. p005 p p<0,05 0 05 (p<0,05) Resultados Observouse Observou se rs229751 p=0,037 p0037 037 (p=0,037 p=0,001 p0001 001 (p=0,001) p<0,001 (p<0,00 modelos. rs3397770 p=0,045 p0045 045 (p=0,045 rs24332 p=0,019, p0019 p=0,019 019 (p=0,019) Conclusões rs1756 rs130403 rs180087 rs900 rs277924 rs289751 (TE 1 OHIP1 (OHIP-1 p00 p<0,0 (p<0,05 rs22975 p=0,03 p003 03 (p=0,03 p=0,00 p000 00 (p=0,001 p<0,00 (p<0,0 rs339777 p=0,04 p004 04 (p=0,04 rs2433 p001 p=0,01 01 (p=0,019 rs175 rs13040 rs18008 rs90 rs27792 rs28975 (OHIP- p0 p<0, rs2297 p=0,0 (p=0,0 (p=0,00 (p<0, rs33977 rs243 (p=0,01 rs17 rs1304 rs1800 rs9 rs2779 rs2897 (OHIP p<0 rs229 p=0, (p=0, (p<0 rs3397 rs24 rs1 rs130 rs180 rs277 rs289 p< rs22 p=0 (p=0 (p< rs339 rs2 rs13 rs18 rs27 rs28 p= (p= (p rs33 rs3

Abstract The aim of this study was to evaluate whether polymorphisms in SOD2 and SOD3 genes modulate the oral health-related quality of life (OHRQoL) of Para athletes with dental caries experience. The cross-sectional study included 264 Para athletes (143 in athletics, 61 in weightlifting and 60 in swimming). A trained and calibrated team recorded the decayed, missing and filled teeth index (DMFT). The Brazilian version of the Oral Health Impact Profile (OHIP-14) was used to measure OHRQoL. Genomic DNA was extracted from the athletes’ saliva, and genetic polymorphisms in the SOD2 (rs5746136 and rs10370) and SOD3 (rs2855262 and rs13306703) genes were analyzed by real-time polymerase chain reaction. Univariate and multivariate analyses were performed. A multivariate General Linear Model analysis, adjusted for sex, revealed that the SOD3 gene polymorphism (rs2855262) had a significant effect on the psychological disability domain [codominant (p = 0.045) and recessive (p=0.038) models]. The SOD2 gene polymorphism (rs5746136) had a significant effect on the total OHIP-14 score [dominant model (p = 0.038)] and the psychological discomfort [dominant model (p = 0.034)] and physical disability [codominant model (p=0.037)] domains. Presence of the SOD2 rs10370 polymorphism led to statistical differences in the total score [codominant (p = 0.026) and dominant (p = 0.023) models] and the handicap domain scores [codominant (p = 0.027) and dominant (p = 0.032) models]. Polymorphisms of the SOD2 and SOD3 genes may be important biomarkers of OHRQoL in Para athletes with dental caries experience. SOD healthrelated health related (OHRQoL experience crosssectional cross sectional 26 143 (14 athletics 6 swimming. swimming . swimming) decayed DMFT. DMFT (DMFT) OHIP14 OHIP 14 (OHIP-14 saliva rs5746136 rs (rs574613 rs2855262 (rs285526 rs13306703 realtime real time reaction performed analysis sex codominant p 0.045 0045 0 045 p=0.038 p0038 038 (p=0.038 models. models OHIP-1 0.038 0038 0.038) 0.034 0034 034 0.034) p=0.037 p0037 037 (p=0.037) domains rs1037 0.026 0026 026 0.023 0023 023 0.027 0027 027 0.032 0032 032 2 (1 (DMFT OHIP1 1 (OHIP-1 rs574613 (rs57461 rs285526 (rs28552 rs1330670 0.04 004 04 p=0.03 p003 03 (p=0.03 OHIP- 0.03 003 (p=0.037 rs103 0.02 002 02 ( (OHIP- rs57461 (rs5746 rs28552 (rs2855 rs133067 0.0 00 p=0.0 p00 (p=0.0 rs10 (OHIP rs5746 (rs574 rs2855 (rs285 rs13306 0. p=0. p0 (p=0. rs1 rs574 (rs57 rs285 (rs28 rs1330 p=0 (p=0 rs57 (rs5 rs28 (rs2 rs133 p= (p= rs5 (rs rs2 rs13

ABSTRACT Objective: To evaluate the influence of antimicrobial photodynamic therapy (aPDT) on the bond strength of adhesive restorative materials to healthy and caries-affected dentin. Material and Methods: 04 electronic databases and 02 additional searches were assessed. In vitro studies considering sound or caries-affected human or bovine dentin (population), use of aPDT before restorative treatment (intervention), use of other dentin disinfection techniques (comparison), and bond strength to dentin (outcome) were included. The risk of bias for in vitro studies was used as a method to check the evidence. The method used to present and synthesize results was data extraction. Results: The total number of included studies was 11, including 550 permanent teeth. Bond strength was evaluated mainly through the shear bond strength (SBS) test, with dentin affected by caries being the substrate most frequently used in studies. Six studies demonstrated that aPDT with methylene blue (MB) negatively affected the SBS values of resin-modified glass ionomer cement (RMGIC) to dentin. Regarding risk of bias assessment, all studies were at high risk of bias. Conclusion: The effects of aPDT on adhesion to dentin vary depending on the type of substrate and the photosensitizer used. Mainly, MB negatively affected the SBS values from RMGIC to dentin. The conclusion should be interpreted considering the high risk of bias in the studies. Objective (aPDT cariesaffected Methods 0 assessed population, population , (population) intervention, intervention (intervention) comparison, comparison (comparison) outcome (outcome evidence extraction Results 11 55 teeth (SBS test (MB resinmodified resin modified (RMGIC assessment Conclusion Mainly (population (intervention (comparison 1 5

ABSTRACT Objective: To perform a bibliometric analysis on deciduous and permanent eruption publications to discuss the global trends and prospects on the topic. Material and Methods: A systematic search was conducted on the Scopus database. The characteristics of the publications, including co-cited authors, organizations, countries, most cited sources, publications, and keyword co-occurrence, were analyzed using VOSviewer software version 1.6.18. Results: After applying exclusion criteria, 492 studies were included in the analysis. The majority of research centers were located in Europe. "Archives of Oral Biology" emerged as the most frequently cited journal. The United States and Brazil were the most frequently cited countries in the publications. Notable co-authors included Kuchler E. C. and Hägg U. The most frequently cited keywords were associated with oral pathologies, tooth development, odontogenesis, and genetics. The most recent papers were published in journals focused on oral diseases. Conclusion: This bibliometric analysis highlights that the future of research in this field is likely to be focused on the investigation of tooth eruption in both deciduous and permanent teeth, with an emphasis on genetics, tooth development, timing of tooth eruption, and syndromic conditions. While well-accepted in the fields of Pediatrics and Orthodontics, there is a growing interest in this topic within oral biology and pathology journals in the Americas, with the United States and Brazil leading in terms of publications and citations. Objective Methods database cocited co authors organizations sources cooccurrence, cooccurrence occurrence, occurrence co-occurrence 1618 1 6 18 1.6.18 Results criteria 49 Europe Archives Biology journal coauthors E C U pathologies development odontogenesis genetics diseases Conclusion teeth conditions wellaccepted well accepted Orthodontics Americas citations 161 1.6.1 4 16 1.6. 1.6 1.

ABSTRACT Objective: To evaluate the impact of endodontic treatment with photobiomodulation on oral health-related quality of life (HRQoL). Material and Methods: Seventy participants with single-rooted teeth and a diagnosis of asymptomatic apical periodontitis were selected and randomized into two groups: control group (CG, n = 35), root canal treatment without additional treatment, and experimental group (EG, n = 35), root canal treatment associated with antimicrobial photodynamic therapy and low-level laser therapy. OHRQoL was assessed using the Oral Health Impact Profile Questionnaire (OHIP-14) at baseline and 7 and 30 days after treatment. Data were subjected to Wilcoxon, Mann-Whitney tests, and linear regression using the generalized estimating equation model, using the Statistical Package for Social Science software with a significance level of 5%. Results: No statistically significant differences were found between groups regarding sociodemographic and clinical characteristics (p>0.05). There was also no difference in the OHRQoL between the CG and EG (p>0.05). However, there was a statistical difference in OHIP-14 scores between baseline and the 7- and 30-day follow-up intervals in both groups for all domains (p<0.05). Conclusion: Root canal treatment, with or without photobiomodulation, significantly improved the OHRQoL of participants after 7 and 30 days of treatment. Objective healthrelated health related HRQoL. HRQoL . (HRQoL) Methods singlerooted single rooted CG, (CG 35, 35 , 35) EG, (EG lowlevel low OHIP14 OHIP 14 (OHIP-14 3 Wilcoxon MannWhitney Mann Whitney tests model 5 5% Results p>0.05. p005 p p>0.05 0 05 (p>0.05) However OHIP-1 30day day followup follow up p<0.05. p<0.05 (p<0.05) Conclusion (HRQoL OHIP1 1 (OHIP-1 p00 p>0.0 (p>0.05 OHIP- p<0.0 (p<0.05 (OHIP- p0 p>0. (p>0.0 p<0. (p<0.0 (OHIP p>0 (p>0. p<0 (p<0. p> (p>0 p< (p<0 (p> (p< (p

ABSTRACT Objective: To compare postoperative pain, tenderness, edema, and medication use after foraminal enlargement in a single visit, using 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine gel (CHX). Material and Methods: Seventy patients with single-rooted teeth with apical periodontitis were randomized into two groups according to the irrigation protocol: control (NaOCl - 35 patients) and case (CHX - 35 patients). Pain intensity was assessed using the visual analog scale (recorded every day for seven days, on the 14th and 30th days after treatment). Edema was assessed by two independent evaluators on the 2nd, 3rd, and 7th days after treatment using photographs. Data were analyzed using Chi-square, Fisher's Exact, and Mann-Whitney U tests in SPSS. Results: There was a statistically significant difference in postoperative pain and tenderness between the groups on the 2nd day (p<0.05). There was no difference between the groups for edema (p=1.00) and use of medication (p=0.77). Conclusion: Chlorhexidine resulted in more significant postoperative pain and tenderness after foraminal enlargement. Objective visit 25 2 5 2.5 NaOCl CHX. CHX . (CHX) Methods singlerooted rooted protocol 3 patients. recorded th treatment. treatment) nd 3rd rd photographs Chisquare, Chisquare Chi square, square Chi-square Fishers Fisher s Exact MannWhitney Mann Whitney SPSS Results p<0.05. p005 p p<0.05 0 05 (p<0.05) p=1.00 p100 1 00 (p=1.00 p=0.77. p077 p=0.77 77 (p=0.77) Conclusion 2. p00 p<0.0 (p<0.05 p=1.0 p10 (p=1.0 p07 p=0.7 7 (p=0.77 p0 p<0. (p<0.0 p=1. p1 (p=1. p=0. (p=0.7 p<0 (p<0. p=1 (p=1 p=0 (p=0. p< (p<0 p= (p= (p=0 (p< (p

ABSTRACT Objective: This study aimed to analyze the scientific production of genetic polymorphisms and external apical root resorption (EARR) to establish main findings, geographic trends, and research gaps for possible future investigations. Methods: Unrestricted publications were searched using the Scopus database (March 2023) to include studies that addressed the association between genetic polymorphisms and EARR. Case-control, cohort, cross-sectional, and review studies were considered eligible. The softwares VOS viewer™ and Bibliometrix were used for data analysis. Results: Of the 44 studies analyzed, “Iglesias-Linares A” was the most cited author. The University of Seville (Spain) conducted the most research on this topic. Brazil, Spain, and the USA were the leading countries in terms of citations. The most frequent term in the co-occurrence of keywords was “EARR.” The journal American Journal of Orthodontics and Dentofacial Orthopedics presented a great relevance in the area, demonstrating a high number of publications. Several genetic polymorphisms have been investigated, with interleukins being the most studied. Conclusion: Endodontics is an area of research that should focus more on root resorption and genetic polymorphisms, as it still underexplored, compared to orthodontics. Polymorphisms have been studied as possible predictors of EARR caused by orthodontic tooth movement. However, the gap in the research indicates a need to search for new genes associated with EARR. Objective (EARR findings trends investigations Methods March 2023 Casecontrol, Casecontrol Case control, control Case-control cohort crosssectional, crosssectional cross sectional, sectional cross-sectional eligible viewer analysis Results 4 analyzed IglesiasLinares Iglesias Linares A author Spain (Spain topic Brazil citations cooccurrence co occurrence “EARR. investigated Conclusion underexplored orthodontics movement However 202 “EARR 20 2

RESUMO Objetivo: Este estudo objetivou analisar a produção científica sobre polimorfismo genético e reabsorção radicular apical externa (external apical root resorption, EARR) para estabelecer os principais achados, tendências geográficas e lacunas de pesquisa, para possíveis investigações futuras. Métodos: Foram pesquisadas publicações na base de dados Scopus (março de 2023) para incluir estudos que abordassem a associação entre polimorfismos genéticos e EARR. Foram considerados elegíveis estudos de caso-controle, relatos de caso, coorte, transversais e revisões. Para análise dos dados, foram utilizados os softwares VOS viewer® e Bibliometrix. Resultados: Dos 44 estudos incluídos, Iglesias-Linares A foi o autor mais citado. A Universidade de Sevilha (Espanha) foi a instituição que mais pesquisou o tema. Brasil, Espanha e EUA foram os principais países em termos de citações. O termo mais frequente na coocorrência das palavras-chave foi EARR. O periódico American Journal of Orthodontics and Dentofacial Orthopedics apresentou grande relevância na área, com vários artigos publicados. Vários polimorfismos genéticos foram investigados, sendo as interleucinas as mais estudadas. Conclusão: Endodontia é uma área de pesquisa que poderia focar mais no tema reabsorção radicular e polimorfismos genéticos, por ser um assunto ainda pouco explorado, em comparação à Ortodontia. Polimorfismos têm sido estudados como possíveis genes preditores de EARR como consequência da movimentação dentária ortodôntica. No entanto, ainda existe uma lacuna na investigação, indicando a necessidade de procurar novos genes associados à EARR. Objetivo external resorption achados futuras Métodos março 2023 casocontrole, casocontrole caso controle, controle caso-controle coorte revisões viewer Bibliometrix Resultados 4 incluídos IglesiasLinares Iglesias Linares citado (Espanha Brasil citações palavraschave palavras chave publicados investigados estudadas Conclusão explorado Ortodontia ortodôntica entanto investigação 202 20 2

This study aims to understand the global panorama of ankyloglossia in newborns by means of a bibliometric analysis of scientific productions up to January 2023. A search was conducted with the terms Newborn, Ankyloglossia and their synonyms in the Pubmed, Embase, Cochrane, Virtual Health Library, Web of Science and Scopus platforms. After removing duplicates and applying the eligibility criteria, 431 studies were included and imported into the VOSviwer software. The most cited keywords were Surgery (198) and Breastfeeding (151). Among the 49 countries surveyed, the United States published the most on the subject (107). The most relevant study in the studied portfolio was Ankyloglossia: assessment, incidence, and effect of frenuloplasty on the breastfeeding dyad by Ballard and collaborators. The journal Pediatrics obtained the highest number of citations (608) and year 2019 recorded the most articles about ankyloglossia in newborns (44). The findings of this study show that the topic linked to oral brakes has sparked a growing interest in the scientific community, and show the concern in unveiling the real impact of ankyloglossia on the infant's breastfeeding, as well as the need to seek a consensus in diagnosis regarding surgical indication.

Este trabalho buscou mapear o panorama global da anquiloglossia em recém-nascidos por meio da análise bibliométrica de produções científicas até janeiro de 2023. Para obtenção dos estudos, foi realizada uma busca avançada com os termos “Newborn”, “Ankyloglossia” e seus sinônimos nas plataformas Pubmed, Embase, Cochrane, Biblioteca Virtual de Saúde, Web of Science e Scopus. Os resultados foram importados para o gerenciador de referência Zotero. Após a remoção das duplicatas e aplicação dos critérios de elegibilidade, 431 estudos foram incluídos na análise e importados para o software VOSviwer versão 16.18. As palavras-chave mais citadas na pesquisa foram “Cirurgia” (198) e “Aleitamento” (151), que também apresentaram forte ligação entre si. Dentre os 49 países pesquisados, os Estados Unidos, o Reino Unido e a Austrália foram os que mais publicaram sobre o assunto, com 107, 44 e 28, respectivamente. O estudo mais relevante no portfólio estudado foi escrito por Ballard e colaboradores sob o título “Ankyloglossia: assessment, incidence, and effect of frenuloplasty on the breastfeeding dyad”. A revista “Pediatrics” obteve o maior número de citações (608) e ano de 2019 registrou a maior quantidade de artigos sobre a anquiloglossia em recém-nascidos (44). Os achados deste estudo demonstram que a temática ligada aos freios orais tem despertado um interesse crescente na comunidade científica e evidenciam a preocupação em desvendar o impacto real da anquiloglossia na amamentação do bebê, assim como a necessidade de buscar um consenso no diagnóstico em relação à indicação cirúrgica.

Abstract This study aimed to assess whether genetic polymorphisms in MTR and MTRR are potential biomarkers of oral health-related quality of life (OHRQoL) in children with caries. A cross-sectional study was designed wherein pairs of parents/caregivers and children (aged two–five years) were selected. Clinical examination was used to detect dental caries, which were classified as low-severity and high-severity caries. The Early Childhood Oral Health Impact Scale (ECOHIS) questionnaire was used to assess OHRQoL. Genomic DNA extracted from the saliva was used to analyze two missense genetic polymorphisms: MTR (rs1805087) and MTRR (rs1801394). Mann–Whitney non-parametric test was used to analyze candidate genes with OHRQoL scale and domain, with a significance level of p≤0.05. MTR (rs1805087) was found associated (p = 0.05) with children’s OHRQoL subscale scores in the dominant model (GG + AG). Genetic polymorphisms in MTR may increase the risk of poor OHRQoL in children with caries. Further studies are needed to investigate genetics, molecular factors, and OHRQoL. healthrelated health related (OHRQoL caries crosssectional cross sectional parentscaregivers parents caregivers aged twofive five years selected lowseverity low severity highseverity high ECOHIS (ECOHIS rs1805087 rs (rs1805087 rs1801394. rs1801394 . (rs1801394) MannWhitney Mann Whitney nonparametric non parametric domain p005 p 0 05 p≤0.05 0.05 005 childrens s GG AG. AG AG) genetics factors rs180508 (rs180508 rs180139 (rs1801394 p00 p≤0.0 0.0 00 rs18050 (rs18050 rs18013 (rs180139 p0 p≤0. 0. rs1805 (rs1805 rs1801 (rs18013 p≤0 rs180 (rs180 (rs1801 p≤ rs18 (rs18 rs1 (rs1 (rs

Abstract An increasing number of systematic reviews (SR) has investigated the association between dental caries and nutritional status in children and adolescents, thus requiring an overview to compile the information in a single piece of evidence. Therefore, this study aimed to evaluate and summarize evidence from published SR on the association between dental caries and nutritional status in children and adolescents. A wide search was conducted on May 29, 2023, in six databases (Medline via PubMed, Scopus, Web of Science, Cochrane library, Embase, and the Virtual Health Library – VHL). An additional search was performed in the gray literature (Open grey and Google Scholar), SR registration databases, and the list of references of the included SR. Our inclusion criteria were based on acronym PECOS. Overall, two reviewers independently extracted the data, evaluated the risk of bias (ROBIS), and assessed the quality of the chosen studies (AMSTAR-2). Data from the included meta-analysis were summarized and certainty of evidence using the GRADE approach was performed. After removing duplicates and applying our eligibility criteria, 19 SR from 2006-2022 were included. We found that 17 SR showed high risk of bias and critically low methodological quality. We observed an association between dental caries experiences and nutritional status since seven SR found an association between obesity/overweight and dental caries; one, an association between underweight and dental caries; and eleven, no associations. The meta-analysis showed divergent results according to the study designs, used indices, and participants’ age group, and were scored as having a very low certainty of evidence. Therefore, based on the high risk of bias, low methodological quality, and very low certainty of evidence of the chosen SR, most studies found no association between children and adolescents’ nutritional status and dental caries experience. (SR adolescents Therefore 29 2023 Medline PubMed Scopus Science library Embase VHL. VHL . VHL) Open Scholar, Scholar , Scholar) PECOS Overall data ROBIS, ROBIS (ROBIS) AMSTAR2. AMSTAR2 AMSTAR 2 (AMSTAR-2) metaanalysis meta analysis 1 20062022 2006 2022 2006-202 obesityoverweight obesity overweight one eleven associations designs indices participants group experience 202 (ROBIS (AMSTAR-2 2006202 200 2006-20 20 (AMSTAR- 200620 2006-2 (AMSTAR 20062 2006-

Resumo Este estudo teve como objetivo avaliar a associação das variáveis idade, sexo, posição no arco, comprimento do dente, amplitude do canal radicular e tamanho da lesão periapical com a ocorrência de sinais e sintomas pós-operatórios (dor, dor ao toque e edema) e o uso de analgésicos após o tratamento endodôntico com alargamento foraminal em dentes uniradiculares com lesão periapical. Este estudo longitudinal prospectivo incluiu 105 pacientes que necessitavam de tratamento endodôntico em dentes uniradiculares superiores ou inferiores com lesão periapical. Após o tratamento do canal radicular em uma sessão, a intensidade da dor e a dor ao toque foram registradas diariamente por 7 dias e nos dias 14 e 30. O edema foi avaliado por dois avaliadores independentes dentro de 48 h, 72 h e 7 dias após o tratamento. Foram realizadas regressões ordinal e logística, e a significância estatística foi fixada em um valor de p < 0,05. Gênero feminino (beta = 1,02; p < 0.01), dentes inferiores (beta = 25,50; p < 0.01), amplitude média do canal radicular (beta = 0,93; p = 0,03) e edema (beta = 1,88; p < 0.01) foram associados ao aumento da dor e dor ao toque pós-operatória, enquanto o uso de analgésicos (beta = -1,82; p < 0.01) e o tempo em dias (beta = -0,23; p < 0.01) foram associados à diminuição desses sinais e sintomas. O edema foi considerado fator de risco para necessidade de analgésico (Odds Ratio [OR] = 61,46; p < 0.01). Fatores como sexo, posição do arco e amplitude do canal radicular foram associados aos sinais e sintomas pós-operatórios. O uso de analgésicos foi mais necessário no edema e foi associado à diminuição da dor.

Abstract This study aimed to evaluate the association of the variables age, gender, arch position, tooth length, root canal amplitude, and periapical lesion size with the occurrence of postoperative signs and symptoms (pain, tenderness, and edema) and the use of postoperative analgesics following root canal treatment with foraminal enlargement in single-rooted teeth with apical periodontitis. This prospective longitudinal study included 105 patients requiring root canal treatment of maxillary or mandibular single-rooted teeth with periapical lesion. After root canal treatment in a single session, pain intensity and tenderness were recorded daily for 7 days and on days 14 and 30. Edema was evaluated by two independent evaluators within 48 h, 72 h, and 7 days after treatment. Ordinal and logistic regressions were performed (p < 0.05). Female gender (beta = 1.02; p < 0.01), mandibular teeth (beta = 25.50; p < 0.01), medium root canal amplitude (beta = 0.93; p = 0.03), and edema (beta = 1.88; p < 0.01) were associated with increased postoperative pain and tenderness, while the use of analgesics (beta = -1.82; p < 0.01) and time in days (beta = -0.23; p < 0.01) were associated with a decrease in these signs and symptoms. Edema was considered a risk factor for analgesic requirement (Odds Ratio [OR] = 61.46; p < 0.01). Factors such as gender, arch position, and root canal amplitude were associated with postoperative signs and symptoms. The use of analgesics was more required in edema and was associated with decreased pain.

Abstract: This cross-sectional study aimed to assess if genetic polymorphisms in TNF- α are associated with a negative impact on Oral Health-Related Quality of Life (OHRQoL) in children with dental caries. A total of 307 pairs of parents/caregivers and children aged two to five years were selected. The children were clinically evaluated and classified according to caries experience and severity of active caries. The Brazilian Portuguese version of the Early Childhood Oral Health Impact Scale (ECOHIS) was used to assess OHRQoL. Genotyping analysis of genetic polymorphisms in TNF- α (rs1799724, rs1799964, and rs1800629) was performed using real-time polymerase chain reaction. In the recessive model, children with the CC genotype of TNF-α (rs1799964) had a significantly high chance of poor OHRQoL in the symptom domain (pain), in both the caries experience (p = 0.045) and the high-severity active caries phenotypes (p = 0.033) (Mann-Whitney U test). It was concluded that genetic polymorphisms in TNF-α are associated with OHRQoL related to the symptom domain (pain), suggesting that TNF-α could be used as a potential biomarker for OHRQoL. Understanding the genetic aspects associated with OHRQoL will allow the early identification of patients with OHRQoL disparities and provide personalized healthcare.

Resumo Este estudo avaliou a associação entre polimorfismos em genes que codificam os receptores de estrogênio 1 (ESR1) e 2 (ESR2), receptor de vitamina D (VDR) e no microRNA17 (que se liga à ESR1 e VDR) e a periodontite apical persistente (PAP) após o tratamento endodôntico. Foram incluídos 162 pacientes com tratamento endodôntico concluído há pelo menos um ano e que apresentavam periodontite apical no início da terapia endodôntica. Exames clínicos e radiográficos foram realizados para avaliar a presença de PAP ou tecidos perirradiculares saudáveis (cicatrizados). As amostras de saliva foram coletadas como fonte de DNA genômico. A genotipagem de ESR1 (rs2234693 e rs9340799), ESR2 (rs1256049 e rs4986938), VDR (rs739837 e rs2228570) e miRNA17 (rs4284505) foram realizadas por PCR em tempo real. O teste do qui-quadrado foi utilizado para a distribuição das frequências genotípicas e alélicas. A análise de haplótipos também foi realizada. Oitenta e nove pacientes foram incluídos no grupo “curado” e 73 no grupo “PAP”. Não foi encontrada associação entre os polimorfismos alélicos e genotípicos estudados e a PAP (p>0,05). Concluí-se que os polimorfismos genéticos em ESR1, ESR2, VDR e miRNA17 não estão associados à PAP.

Abstract This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.

Abstract Objective: To evaluate traumatic dental injuries (TDI) in primary teeth and the association of gender and age with different injuries. Material and Methods: Records of patients with TDI in primary teeth were included. The following parameters were registered: gender and age, place of trauma, cause of trauma, affected tissue and tooth, number of injured teeth, type of injury, and gingival and bone damage. A logistic regression analysis was performed to explore the interaction between gender and age on the occurrence of types of injuries (p≤0.05). Results: The total of 721 records were evaluated and 370 records were included, being 61.6% boys and 60.5% children aged 0-3 years old, with 658 primary teeth affected. The support tissue was most affected (496/658), followed by dental tissue (139/658). Enamel/dentine fracture with pulp exposure (n=51) and intrusion (n=131) were the most common injuries of dental and support tissues, respectively. In general, boys suffered more traumas than girls, regardless of the age range. As for concussion, logistic regression confirms that gender and age are also influencers. Girls (OR=1.822, CI = 1.050-3.164, p=0.033) in the 4-6 year age group (OR=2.15, CI = 1.239-3.747, p=0.007) are more likely to have concussions. Children age 4-6 years were less likely to suffer an intrusion (OR=0.496; CI = 0.278-0.886; p=0.018). Conclusion: Gender and age influence concussion and intrusion in the primary teeth.

Abstract This study was performed to evaluate the interplay between dental caries, nutritional status, and genetic polymorphisms in TAS1R1 and TAS1R2 (taste receptor, type 1, member 1 and 2) in preschool children and pre-adolescents. We included 525 subjects (306 preschool children and 219 pre-adolescents). Parents/caregivers answered a self-administered questionnaire about their children’s systemic health, characteristics, oral hygiene habits, and diet. Clinical examination was performed to evaluate dental caries and nutritional status. Saliva samples were collected for DNA extraction. The genotyping of rs17492553 ( TAS1R1 ), rs3935570, and rs4920566 ( TAS1R2 ) polymorphisms was performed using real-time PCR with Taqman Genotyping Master Mix and SNP assay. Both univariate and multivariate Poisson regression analyses with robust variance were used for the data analysis. In preschool children, consumption of sweets between meals increased the prevalence of dental caries by 85% (PR c = 1.85; 95%CI 1.39–2.46; p < 0.001), whereas in pre-adolescents, this prevalence increased by 34% (PR a = 1.34; 95%CI 1.11–1.62; p = 0.002), regardless of genetic polymorphisms . Moreover, individuals carrying at least one allele C in rs17492553 presented 23% more prevalence of dental caries (PR a = 1.23; 95%CI 1.02–1.49 p = 0.030). Nutritional status was not associated with dental caries, neither with genetic polymorphisms . Consumption of sweets between meals increased the prevalence of dental caries. In pre-adolescents, rs17492553 genetic polymorphism in TAS1R1 was associated with dental caries.