ABSTRACT Objective: To examine the physical function and respiratory muscle strength of patients - who recovered from critical COVID-19 – after intensive care unit discharge to the ward on Days one (D1) and seven (D7), and to investigate variables associated with functional impairment. Methods: This was a prospective cohort study of adult patients with COVID-19 who needed invasive mechanical ventilation, non-invasive ventilation or high-flow nasal cannula and were discharged from the intensive care unit to the ward. Participants were submitted to Medical Research Council sum-score, handgrip strength, maximal inspiratory pressure, maximal expiratory pressure, and short physical performance battery tests. Participants were grouped into two groups according to their need for invasive ventilation: the Invasive Mechanical Ventilation Group (IMV Group) and the Non-Invasive Mechanical Ventilation Group (Non-IMV Group). Results: Patients in the IMV Group (n = 31) were younger and had higher Sequential Organ Failure Assessment scores than those in the Non-IMV Group (n = 33). The short physical performance battery scores (range 0 - 12) on D1 and D7 were 6.1 ± 4.3 and 7.3 ± 3.8, respectively for the Non-Invasive Mechanical Ventilation Group, and 1.3 ± 2.5 and 2.6 ± 3.7, respectively for the IMV Group. The prevalence of intensive care unit-acquired weakness on D7 was 13% for the Non-IMV Group and 72% for the IMV Group. The maximal inspiratory pressure, maximal expiratory pressure, and handgrip strength increased on D7 in both groups, but the maximal expiratory pressure and handgrip strength were still weak. Only maximal inspiratory pressure was recovered (i.e., > 80% of the predicted value) in the Non-IMV Group. Female sex, and the need and duration of invasive mechanical were independently and negatively associated with the short physical performance battery score and handgrip strength. Conclusion: Patients who recovered from critical COVID-19 and who received invasive mechanical ventilation presented greater disability than those who were not invasively ventilated. However, they both showed marginal functional improvement during early recovery, regardless of the need for invasive mechanical ventilation. This might highlight the severity of disability caused by SARS-CoV-2. Objective COVID19 COVID 19 COVID-1 D (D1 D7, , (D7) impairment Methods noninvasive non highflow high flow sumscore, sumscore sum score, sum-score tests NonInvasive Non NonIMV . Results n 31 33. 33 33) range 12 61 6 1 6. 43 4 3 4. 73 7 7. 38 8 3.8 13 1. 25 2 5 2. 26 37 3.7 unitacquired acquired 72 weak i.e., ie i e (i.e. 80 value sex Conclusion ventilated However recovery SARSCoV2. SARSCoV2 SARSCoV SARS CoV SARS-CoV-2 COVID1 COVID- (D (D7 3. i.e. (i.e SARS-CoV- i.e SARS-CoV

RESUMO Objetivo: Examinar a função física e a força muscular respiratória de pacientes que se recuperaram da COVID-19 grave após a alta da unidade de terapia intensiva para a enfermaria nos Dias 1 e 7 e investigar as variáveis associadas ao comprometimento funcional. Métodos: Trata-se de estudo de coorte prospectivo de pacientes adultos com COVID-19 que necessitaram de ventilação mecânica invasiva, ventilação mecânica não invasiva ou cânula nasal de alto fluxo e tiveram alta da unidade de terapia intensiva para a enfermaria. Os participantes foram submetidos aos testes Medical Research Council sum-score, força de preensão manual, pressão inspiratória máxima, pressão expiratória máxima e short physical performance battery. Os participantes foram agrupados em dois grupos conforme a necessidade de ventilação mecânica invasiva: o Grupo Ventilação Mecânica Invasiva (Grupo VMI) e o Grupo Não Ventilação Mecânica Invasiva (Grupo Não VMI). Resultados: Os pacientes do Grupo VMI (n = 31) eram mais jovens e tinham pontuações do Sequential Organ Failure Assessment mais altas do que os do Grupo VMI (n = 33). As pontuações do short physical performance battery (intervalo de zero a 12) nos Dias 1 e 7 foram 6,1 ± 4,3 e 7,3 ± 3,8, respectivamente para o Grupo Não VMI, e 1,3 ± 2,5 e 2,6 ± 3,7, respectivamente para o Grupo VMI. A prevalência de fraqueza adquirida na unidade de terapia intensiva no Dia 7 foi de 13% para o Grupo Não VMI e de 72% para o Grupo VMI. A pressão inspiratória máxima, a pressão expiratória máxima e a força de preensão manual aumentaram no Dia 7 em ambos os grupos, porém a pressão expiratória máxima e a força de preensão manual ainda eram fracas. Apenas a pressão inspiratória máxima foi recuperada (ou seja, > 80% do valor previsto) no Grupo Não VMI. As variáveis sexo feminino, e necessidade e duração da ventilação mecânica invasiva foram associadas de forma independente e negativa à pontuação do short physical performance battery e à força de preensão manual. Conclusão: Os pacientes que se recuperaram da COVID-19 grave e receberam ventilação mecânica invasiva apresentaram maior incapacidade do que aqueles que não foram ventilados invasivamente. No entanto, os dois grupos de pacientes apresentaram melhora funcional marginal durante a fase inicial de recuperação, independentemente da necessidade de ventilação mecânica invasiva. Esse resultado pode evidenciar a gravidade da incapacidade causada pelo SARS-CoV-2. Objetivo COVID19 COVID 19 COVID-1 Métodos Tratase Trata sumscore, sumscore sum score, score sum-score . Resultados n 31 33. 33 33) intervalo 12 61 6 6, 43 4 3 4, 73 7, 38 8 3,8 13 1, 25 2 5 2, 26 37 3,7 72 fracas seja 80 previsto feminino Conclusão invasivamente entanto recuperação SARSCoV2. SARSCoV2 SARSCoV SARS CoV 2. SARS-CoV-2 COVID1 COVID- 3, SARS-CoV- SARS-CoV

ABSTRACT The aim of this study was to assess the internal quality of free-range eggs available for sale in Alfenas – MG, Brazil, and stored under refrigerated conditions (maximum and minimum temperatures of 9.7 and 6.7 °C, respectively). The experimental design was completely randomized, structured as a 2x5 factorial arrangement involving two types of retail outlets (supermarket (S) and open-air market (OA)) and five storage periods (1, 8, 15, 22, and 29 days), resulting in 10 treatments. Each treatment had six replicates, represented by individual eggs. The following parameters were evaluated: Haugh unit (HU); percentage of egg weight loss (WL%); percentages of albumen, yolk, and shell; yolk index (YI); albumen index (AI); yolk pH; and yolk color. Egg weight loss increased (p<0.05) with the length of the storage period. Eggs acquired from S were deemed to be of inferior quality (HU < 60), in contrast to those obtained from OA (p<0.05). A significant interaction effect was noted for YI (p<0.05), with eggs from S exhibiting higher YI values than those from OA at 15 and 22 days of storage. Additionally, eggs from S had diminished AI values (p<0.05) when compared to those acquired from OA. The yolk color in eggs from S was more intense than that in eggs from OA (p<0.05). A lower yolk percentage was detected in eggs from S relative to those from OA (p<0.05). Therefore, eggs sold in Alfenas – MG, Brazil, from open-air markets possess superior quality compared to those sold in supermarkets. freerange free range MG Brazil maximum 97 9 7 9. 67 6 6. C °C respectively. respectively . respectively) randomized x 2x supermarket (S openair open air (OA) 1, 1 (1 8 2 days, , days) treatments replicates evaluated HU (HU) WL% WL (WL%) shell (YI) (AI) pH p<0.05 p005 p 0 05 (p<0.05 period 60, 60 60) p<0.05. p<0.05, Additionally Therefore supermarkets (OA ( (WL% (YI (AI p<0.0 p00 (p<0.0 (WL p<0. p0 (p<0. p<0 (p<0 p< (p< (p

RESUMO Objetivou-se avaliar a qualidade interna de ovos tipo caipira comercializados na cidade de Alfenas – MG, armazenados sob refrigeração (máxima 9,7°C e mínima 6,7°C). Os ovos foram distribuídos em um delineamento inteiramente casualizado, em esquema fatorial 2x5, sendo dois estabelecimentos comerciais (supermercado (S) e feira livre (FL)) e cinco períodos de armazenamento (um, oito, 15, 22, 29 dias) totalizando 10 tratamentos com 6 repetições (ovo) cada. As variáveis analisadas foram unidade Haugh(UH), perda de peso dos ovos (PP%), porcentagem de albúmen, gema e casca, índice de gema (IG) e albúmen (IA), pH de gema e coloração da gema. A PP% aumentou (p<0,05) conforme o período de armazenamento. Os ovos do S foram classificados como de baixa qualidade (UH < 60), diferindo dos ovos da FL (p<0,05). Para o IG observou-se uma interação significativa (p<0,05), de modo que, aos 15 e 22 dias de armazenamento os ovos do S apresentaram maior IG que aqueles provenientes da FL. Os ovos do S apresentaram valores inferiores de IA (p<0,05) em relação aos adquiridos na FL. Ovos do S apresentaram coloração de gema mais acentuada que os ovos comercializados na FL (p<0,05). Constatou-se menor porcentagem de gema dos ovos do S quando comparados com os da FL (p<0,05). Os ovos comercializados na cidade de Alfenas-MG provenientes de feira livre apresentam qualidade superior aos do supermercado. Objetivouse Objetivou se MG máxima 97C C 9 7 6,7°C. 67C 6,7°C . 6,7°C) casualizado 2x5 x supermercado (S (FL) um, (um oito 2 1 ovo (ovo cada HaughUH, HaughUH Haugh UH , Haugh(UH) PP%, PP (PP%) casca (IG IA, (IA) p<0,05 p005 p 0 05 (p<0,05 60, 60 60) p<0,05. observouse observou p<0,05, Constatouse Constatou AlfenasMG 2x (FL Haugh(UH (PP% (IA p<0,0 p00 (p<0,0 (PP p<0, p0 (p<0, p<0 (p<0 p< (p< (p

ABSTRACT Despite being subject to lower AIDS-related mortality rates and having a higher life expectancy, patients with HIV are more prone to develop non-AIDS events. A low CD4+/CD8+ ratio during antiretroviral therapy identifies people with heightened immune senescence and increased risk of mortality. In clinical practice, finding determinants of a low CD4+/CD8+ ratio may be useful for identifying patients who require close monitoring due to an increased risk of comorbidities and death. We performed a prospective study on the evolution of the CD4+/CD8+ ratio in 60 patients infected with HIV (80% males), who were subjected to two different antiretroviral regimens: early and deferred therapy. The initial CD4+/CD8+ ratio was ≤1 for 70% of the patients in both groups. Older age, CD4+ cell count at inclusion, Nadir CD8+T-cell count, and Initial CD4+/CD8+ ratio ≤ 1 were risk factors for lack of ratio recovery. In the multivariate analysis, a CD4+/CD8+ ratio > 1 at the start of the treatment was found to be a determinant factor in maintaining a CD4+/CD8+ ratio > 1. The nadir CD4+T-cell count was lower in the deferred therapy group (p=0.004), and the last CD4+/CD8+ ratio ≤1 was not associated with comorbidities. Ratio recovery was not associated with the duration of HIV infection, time without therapy, or absence of AIDS incidence. A greater improvement was observed in patients treated early (p=0.003). In contrast, the slope of increase was slower in patients who deferred treatment. In conclusion, the increase in the CD4+/CD8+ ratio occurred mostly for patients undergoing early strategy treatment and its extension did not seem to be related to previous HIV-related factors. AIDSrelated expectancy nonAIDS non events CD4CD8 CDCD CD4 CD8 CD CD4+/CD8 practice death 6 80% 80 (80 males, males , males) regimens 70 groups age inclusion CD8+Tcell CD8Tcell CDTcell CD8+T T analysis CD4+Tcell CD4Tcell CD4+T p=0.004, p0004 p p=0.004 0 004 (p=0.004) infection incidence p=0.003. p0003 p=0.003 . 003 (p=0.003) contrast conclusion HIVrelated CD4CD CD4+/CD 8 (8 7 Tcell CD8T CDT CD4T p000 p=0.00 00 (p=0.004 (p=0.003 ( p00 p=0.0 (p=0.00 p0 p=0. (p=0.0 p=0 (p=0. p= (p=0 (p= (p

ABSTRACT The sense of mastery is conceptualized as a positive aspect of care targeted at people living with dementia, a coping mechanism to reduce burden, and may represent a protective factor for caregivers’ mental and physical health. Objective: To translate and culturally adapt the Pearlin Mastery Scale for Brazil. Methods: A methodological study was conducted at the Federal University of São Carlos in which the initial translation stages were followed; synthesis; back-translation; review by the committee of judges by analyzing the Content Validity Index (CVI); and test of the pre-final version. Results: Two specialists translated the scale into Brazilian Portuguese and defined a consensus version with the researchers. Subsequently, another two specialists back-translated the consensus version, which was reviewed by three judges who are PhDs in the area, considering all scale items as very equivalent (CVI=1.0), and maintaining them in the pre-final version of the instrument. This was tested in a first group of caregivers for them to point out adjustments. The suggestions were accepted by modifying three items and, afterward, the scale was tested in a second group, which did not present difficulties answering the instrument. Conclusion: The Pearlin Mastery Scale was translated and culturally adapted for Brazil, showing equivalence. However, future psychometric analyses of the instrument are required to make it available for use in this population. dementia burden health Objective Brazil Methods followed synthesis backtranslation back back-translation CVI (CVI) prefinal pre final Results researchers Subsequently backtranslated area CVI=1.0, CVI10 CVI=1.0 , 1 0 (CVI=1.0) adjustments afterward Conclusion equivalence However population (CVI CVI1 CVI=1. (CVI=1.0 CVI=1 (CVI=1. CVI= (CVI=1 (CVI=

RESUMO O senso de domínio é conceituado como um aspecto positivo do cuidado voltado às pessoas que vivem com demência, um mecanismo de enfrentamento para reduzir a sobrecarga e que pode representar um fator protetor para a saúde mental e física do cuidador. Objetivo: Traduzir e adaptar culturalmente a Pearlin Mastery Scale para o Brasil. Métodos: Estudo metodológico conduzido na Universidade Federal de São Carlos, em que foram seguidas as etapas de tradução inicial; síntese; retrotradução; revisão pelo comitê de juízes pela análise do Índice de Validade de Conteúdo (IVC); e teste da versão pré-final. Resultados: Dois especialistas traduziram a escala para o português brasileiro e definiram uma versão consensual com os pesquisadores. Posteriormente, outros dois especialistas retrotraduziram a versão consensual, que foi revisada por três juízes doutores na área, considerando todos os itens da escala como muito equivalentes (IVC=1,0), mantendo-os na versão pré-final do instrumento. Esta foi testada em um primeiro grupo de cuidadores, a fim de se apontarem adequações. As sugestões foram acatadas mediante a modificação de três itens e, depois, a escala foi testada em um segundo grupo, o qual não apresentou dificuldades em responder ao instrumento. Conclusão: A Pearlin Mastery Scale foi traduzida e adaptada culturalmente para o Brasil, demonstrando equivalência. Entretanto, análises psicométricas futuras do instrumento são necessárias para disponibilizá-lo para uso entre essa população. demência cuidador Objetivo Brasil Métodos Carlos inicial síntese retrotradução IVC (IVC) préfinal. préfinal pré final. final Resultados pesquisadores Posteriormente área IVC=1,0, IVC10 IVC=1,0 , 1 0 (IVC=1,0) mantendoos mantendo cuidadores adequações depois Conclusão equivalência Entretanto disponibilizálo disponibilizá lo população (IVC IVC1 IVC=1, (IVC=1,0 IVC=1 (IVC=1, IVC= (IVC=1 (IVC=

ABSTRACT BACKGROUND: The Positive Aspects of Caregiving (PAC) scale is used to assess psychosocial benefits provided to caregivers by the task of caring. The PAC scale consists of nine items, assessed using a five-point Likert scale, with higher values indicating greater positive perceptions and gains from the caregiving experience. OBJECTIVE: To translate and culturally adapt the PAC scale for informal Brazilian caregivers of people with dementia. DESIGN AND SETTING: A methodological study was conducted at the Federal University of São Carlos. METHODS: The following stages were carried out: Translation; Synthesis of the translations; Back-translation; Evaluation by an experts’ committee; and Pre-test. RESULTS: Two independent professionals translated the PAC scale. The consensus version was obtained by merging both translations, which were back-translated into English by a third translator. The expert committee comprised three specialists in the area and project researchers. All scale items presented a Content Validity Index of 1 (CVI = 1.0), and thus remained in the pre-final version of the instrument. The instrument was pre-tested with seven caregivers of people with dementia, the majority of whom were women (57.1%), with a degree of kinship corresponding to sons/daughters (57.1%) and an average age of 55.2 (± 4.1) years. The caregivers considered it clear and understandable and made no suggestions for changes. CONCLUSION: The PAC scale was translated and culturally adapted for use by informal caregivers of people with dementia in Brazil. However, a psychometric analysis of the instrument is necessary to provide normative data for this population group. BACKGROUND (PAC caring fivepoint five point experience OBJECTIVE SETTING Carlos METHODS out Translation translations Backtranslation Back translation Back-translation experts Pretest. Pretest Pre test. test Pre-test RESULTS backtranslated back translator researchers CVI 1.0, 10 1.0 , 0 1.0) prefinal pre final pretested tested 57.1%, 571 57.1% 57 sonsdaughters sons daughters (57.1% 552 55 2 55. ± ( 4.1 41 4 years changes CONCLUSION Brazil However group 1. 57.1 5 (57.1 4. 57. (57. (57 (5

RESUMEN Objetivo: analizar las estrategias de liderazgo de enfermeros en hospitales universitarios frente a los cambios en la gestión del cuidado en el enfrentamiento de la COVID-19 a la luz de John Kotter. Métodos: investigación multicéntrica, de tipo cualitativa y analítica. Se realizó mediante entrevistas semiestructuradas, con 139 enfermeros líderes, de 10 hospitales universitarios, en Brasil. En el análisis de datos se utilizó el análisis de contenido de Bardin y el software webQDA. Resultados: se obtuvo una categoría principal “Estrategias de liderazgo de enfermería en el enfrentamiento de la COVID-19”, y cinco subcategorías. Destacando el liderazgo de los enfermeros por la importancia de la visión estratégica para enfrentar la COVID-19 dentro de las instituciones hospitalarias, así como la necesidad de trabajar conjuntamente con sus equipos y otros profesionales de la salud. Consideraciones Finales: los resultados evidenciaron las estrategias utilizadas por los enfermeros líderes en el enfrentamiento de la COVID-19, siendo posible relacionarlas con el marco teórico de John Kotter y su modelo de cambio. Objetivo COVID19 COVID 19 COVID-1 Métodos multicéntrica analítica semiestructuradas 13 1 Brasil webQDA Resultados Estrategias COVID19, , COVID-19” subcategorías hospitalarias salud Finales 19, cambio COVID1 COVID-

RESUMO Objetivo: analisar as estratégias de liderança de enfermeiros em hospitais universitários diante das mudanças na gestão do cuidado no enfrentamento da covid-19 à luz de John Kotter. Métodos: pesquisa multicêntrica, do tipo qualitativa e analítica. Ocorreu por meio de entrevistas-semiestruturadas, com 139 enfermeiros-líderes, de 10 hospitais universitários, no Brasil. Na análise de dados utilizou a análise de conteúdo de Bardin e o software webQDA. Resultados: obteve-se uma principal categoria “Estratégias de liderança de enfermeiros no enfrentamento da covid-19”, e cinco subcategorias. Destacando a liderança de enfermeiros pela importância da visão estratégica para enfrentar a covid-19 no interior das instituições hospitalares, bem como a necessidade de trabalhar em conjunto com suas equipes e demais profissionais de saúde. Considerações Finais: os resultados evidenciaram as estratégias utilizadas por enfermeiros-líderes no enfrentamento da covid-19, sendo possível de relacionar com o referencial teórico de John Kotter e seu modelo de mudança. Objetivo covid19 covid 19 covid-1 Métodos multicêntrica analítica entrevistassemiestruturadas, entrevistassemiestruturadas entrevistas semiestruturadas, semiestruturadas entrevistas-semiestruturadas 13 enfermeiroslíderes, enfermeiroslíderes líderes, líderes 1 Brasil webQDA Resultados obtevese obteve se Estratégias covid19, , covid-19” subcategorias hospitalares saúde Finais 19, mudança covid1 covid-

ABSTRACT Objective: To analyze the leadership strategies of nurses in university hospitals in response to care management changes during the COVID-19 pandemic, informed by John Kotter’s insights. Methods: This multicentric study utilized qualitative and analytical methods. It was conducted through semi-structured interviews with 139 lead nurses from 10 university hospitals in Brazil. Data analysis included Bardin’s content analysis and the webQDA software. Results: The primary category identified was “Nursing Leadership Strategies in the Battle Against COVID-19,” encompassing five subcategories. This category underscored the importance of strategic vision in nursing leadership for combating COVID-19 within hospital settings, as well as the necessity of working collaboratively with their teams and other healthcare professionals. Final Considerations: The results highlight the strategies used by lead nurses in confronting COVID-19, which can be associated with John Kotter’s theoretical framework and his model of change. Objective COVID19 COVID 19 COVID-1 pandemic Kotters Kotter s insights Methods methods semistructured semi structured 13 1 Brazil Bardins Bardin software Results Nursing COVID19, 19, subcategories settings professionals Considerations change COVID1 COVID-

Abstract This study aimed to develop a pyriproxyfen-based Rumino-Reticulum Device (RRD) consisting of films of poly(vinyl)alcohol (PVA) and sodium carboxymethylcellulose (NaCMC) to control the horn fly in cattle, one of the major pests of livestock. Films were obtained by the solvent casting method by PVA/NaCMC crosslinking and presented satisfactory homogeneity, drug content (104.8%) and pH (6.5), besides great absorptive capacity with swelling degree of 331.40% after 1 hour and diffusion-controlled release kinetics (Higuchi). FTIR and SEM analyzes clarify the characteristic bands of PVA, NaCMC and pyriproxyfen. The XRD and thermal analysis shows an increase in crystallinity due to pyriproxyfen (Xc:36.59%) and the active delivery alters the chain packing (Tg:74ºC). The pyriproxyfen-based RRD developed, in addition to fulfilling the characteristics of prolonged release, allows it to be rolled up (compressed form) facilitating swallowing and subsequent conversion to an expanded form that is retained in the rumen throughout the treatment period. pyriproxyfenbased based RuminoReticulum Rumino Reticulum (RRD polyvinylalcohol poly vinyl alcohol PVA (PVA (NaCMC cattle livestock PVANaCMC homogeneity 104.8% 1048 104 8 (104.8% 6.5, 65 6.5 , 6 5 (6.5) 33140 331 40 331.40 diffusioncontrolled diffusion controlled Higuchi. Higuchi . (Higuchi) Xc36.59% Xc3659 Xc 36.59% 36 59 (Xc:36.59% Tg74ºC. Tg74ºC TgºC Tg 74ºC ºC (Tg:74ºC) developed compressed period 104.8 10 (104.8 6. (6.5 3314 33 4 331.4 (Higuchi Xc36 Xc36.59 Xc365 3659 36.59 3 (Xc:36.59 (Tg:74ºC 104. (104. (6. 331. Xc3 Xc36.5 365 36.5 (Xc:36.5 (104 (6 Xc36. 36. (Xc:36. (10 ( (Xc:36 (1 (Xc:3 (Xc: (Xc

ABSTRACT Objective: To conduct a serological screening for toxoplasmosis in the heel prick test and to evaluate its epidemiological aspects in newborns and postpartum women in Jataí, Goiás. Method: Cross-sectional epidemiological study for the biological screening of newborns in Jataí, Goiás. Results: The study participants amounted to 228 newborns, whose samples were collected between the third and seventh day of life. IgG antibodies against Toxoplasma gondii were detected in 40.79% (93/228) of the samples; out of these, 23.6% (22/93) had high IgG antibody titers, leading to the collection of two other peripheral blood samples and the detection of a decrease in these titers. Conclusion: The findings show the importance of strengthening actions in primary health care to prevent infection and training health professionals in this area to equip them with information regarding cases of reinfection and reactivation of infection in pregnant women, minimizing risks for babies. Objective Jataí Goiás Method Crosssectional Cross sectional Results 22 life 4079 40 79 40.79 93/228 93228 93 (93/228 236 23 6 23.6 22/93 2293 (22/93 titers Conclusion babies 2 407 4 7 40.7 93/22 9322 9 (93/22 23. 22/9 229 (22/9 40. 93/2 932 (93/2 22/ (22/ 93/ (93/ (22 (93 (2 (9 (

RESUMO Objetivo: Realizar a triagem sorológica da toxoplasmose no teste do pezinho e avaliar os aspectos epidemiológicos em recém-nascidos e puérperas de Jataí, Goiás. Método: Estudo epidemiológico transversal para a triagem biológica de recém-nascidos em Jataí, Goiás. Resultados: Participaram desta pesquisa 228 recém-nascidos, sendo coletadas amostras entre o terceiro e o sétimo dia de vida. Foram detectados anticorpos IgG anti Toxoplasma gondii em 40,79% (93/228) das amostras; destes, 23,6% (22/93) apresentaram altos títulos de anticorpos IgG e tiveram outras duas amostras de sangue periférico coletadas, sendo verificada a diminuição desses títulos. Conclusão: Tais achados demonstraram a importância do fortalecimento de ações junto à atenção primária à saúde para a prevenção da infecção, assim como a capacitação de profissionais da saúde que atuam nesta área, para que sejam munidos de informações referentes a casos de reinfecção e reativação da infecção em gestantes, minimizando os riscos para os bebês. Objetivo recémnascidos recém nascidos Jataí Goiás Método Resultados 22 recémnascidos, nascidos, vida 4079 40 79 40,79 93/228 93228 93 (93/228 destes 236 23 6 23,6 22/93 2293 (22/93 Conclusão área gestantes bebês 2 407 4 7 40,7 93/22 9322 9 (93/22 23, 22/9 229 (22/9 40, 93/2 932 (93/2 22/ (22/ 93/ (93/ (22 (93 (2 (9 (

RESUMEN Objetivo: Realizar un cribado serológico de la toxoplasmosis en la prueba del talón y evaluar sus aspectos epidemiológicos en los recién nacidos y puérperas en Jataí, Goiás. Método: Estudio epidemiológico transversal para el cribado biológico de los recién nacidos en Jataí, Goiás. Resultados: Participaron en el estudio 228 recién nacidos, cuyas muestras fueron recolectadas entre el tercer y séptimo día de vida. Se detectaron anticuerpos IgG contra Toxoplasma gondii en el 40,79% (93/228) de las muestras; de ellos el 23,6% (22/93) presentaban títulos elevados de anticuerpos IgG y de ellos se recogieron otras dos muestras de sangre periférica; se detectó una disminución de estos títulos. Conclusión: Los hallazgos muestran la importancia de fortalecer las acciones en la atención primaria de salud para prevenir la infección y capacitar a los profesionales de la salud en esta área para dotarlos de información sobre los casos de reinfección y reactivación de la infección en mujeres embarazadas, minimizando los riesgos para los bebés. Objetivo Jataí Goiás Método Resultados 22 vida 4079 40 79 40,79 93/228 93228 93 (93/228 236 23 6 23,6 22/93 2293 (22/93 periférica Conclusión embarazadas bebés 2 407 4 7 40,7 93/22 9322 9 (93/22 23, 22/9 229 (22/9 40, 93/2 932 (93/2 22/ (22/ 93/ (93/ (22 (93 (2 (9 (

ABSTRACT Purpose: This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich’s experimental tumor (EET) in mice. Methods: Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 106 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax. Results: The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract. Conclusions: The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells. Purpose L (EEOb Ehrlichs s (EET Methods control treated 6. 6 . 6) 21 10 0.05 005 0 05 (0.0 mL development days AgNOR (AgNOR antigen Bcl2 Bcl Bcl- Bax Results mgkg mg kg Control 37 3 7 3. 01 1 0. vs 57 5 5. 02 Conclusions 0.0 00 (0. (0 (

Abstract Temperature affects the rate of biochemical and physiological processes in amphibians, influencing metamorphic traits. Temperature patterns, as those observed in latitudinal and altitudinal clines, may impose different challenges on amphibians depending on how species are geographically distributed. Moreover, species’ response to environmental temperatures may also be phylogenetically constrained. Here, we explore the effects of acclimation to higher temperatures on tadpole survival, development, and growth, using a meta-analytical approach. We also evaluate whether the latitude and climatic variables at each collection site can explain differences in species’ response to increasing temperature and whether these responses are phylogenetically conserved. Our results show that species that develop at relatively higher temperatures reach metamorphosis faster. Furthermore, absolute latitude at each collection site may partially explain heterogeneity in larval growth rate. Phylogenetic signal of traits in response to temperature indicates a non-random process in which related species resemble each other less than expected under Brownian motion evolution (BM) in all traits, except survival. The integration of studies in a meta-analytic framework allowed us to explore macroecological and macroevolutionary patterns and provided a better understanding of the effects of climate change on amphibians. clines distributed Moreover constrained Here survival development metaanalytical meta analytical approach conserved faster Furthermore nonrandom non random BM (BM metaanalytic analytic

RESUMEN Objetivo: evaluar la calidad de vida laboral de los profesionales sanitarios que prestan atención directa e indirecta a los casos de COVID-19. Métodos: se trata de un estudio transversal llevado a cabo con 156 profesionales sanitarios de un hospital de referencia. Se investigó la relación entre las variables sociodemográficas, laborales y el estrés percibido y los dominios de la Escala de Evaluación de la Calidad de Vida Laboral mediante estadística inferencial y de regresión. Resultados: la satisfacción por Compasión fue moderada (media: 38,2), con nivel de estrés percibido, Burnout y Estrés Traumático Secundario bajos (medias: 18,8, 21,6 y 19,1). Hubo asociaciones entre: escolaridad, sueldo, pluriempleo y atención directa con la Satisfacción por Compasión; bajos ingresos, ser enfermero y hacer horas extras con el Burnout; y trabajar más de 12 horas, enfermedad subyacente y hospitalización por COVID-19 con Estrés Traumático Secundario. Conclusión: la calidad de vida en el trabajo fue satisfactoria, a pesar de la presencia de Burnout y Estrés Traumático Secundario. Objetivo COVID19. COVID19 COVID 19. 19 Métodos 15 referencia sociodemográficas regresión Resultados media (media 38,2, 382 38,2 , 38 2 38,2) medias (medias 188 18 8 18,8 216 21 6 21, 19,1. 191 19,1 . 1 19,1) escolaridad sueldo ingresos COVID-1 Conclusión satisfactoria COVID1 38, 3 18, 19, COVID-

RESUMO Objetivo: avaliar a qualidade de vida no trabalho dos profissionais da saúde em atendimento direto e indireto de casos de covid-19. Métodos: estudo transversal com 156 profissionais da saúde de um hospital de referência. Investigou-se a relação entre variáveis sociodemográficas, laborais e percepção de estresse e domínios da Escala de Avaliação da Qualidade de Vida no Trabalho, por meio de estatística inferencial e de regressão. Resultados: a Satisfação por Compaixão foi moderada (média: 38,2), com percepção de estresse, Burnout e Estresse Traumático Secundário baixos (médias: 18,8, 21,6 e 19,1). Houve associações entre: escolaridade, salário, múltiplos empregos e atendimento direto com o aspecto Satisfação por Compaixão; baixa renda, ser enfermeiro e trabalhar horas extras com Burnout; e trabalhar mais de 12 horas, doença de base e internação por covid-19 com Estresse Traumático Secundário. Conclusão: a qualidade de vida no trabalho foi satisfatória, apesar da presença de Burnout e Estresse Traumático Secundário. Objetivo covid19. covid19 covid 19. 19 Métodos 15 referência Investigouse Investigou se sociodemográficas Trabalho regressão Resultados média (média 38,2, 382 38,2 , 38 2 38,2) médias (médias 188 18 8 18,8 216 21 6 21, 19,1. 191 19,1 . 1 19,1) escolaridade salário renda covid-1 Conclusão satisfatória covid1 38, 3 18, 19, covid-

ABSTRACT Objective: to evaluate the quality of life at work of health professionals in direct and indirect care of COVID-19 cases. Methods: this was a cross-sectional study with 156 health professionals from a referral hospital. The relationship between sociodemographic and work-related variables and perceived stress and domains of the Quality of Life at Work Scale was investigated using inferential statistics and regression. Results: Satisfaction with Compassion was moderate (mean: 38.2), with low perception of stress, Burnout and Secondary Traumatic Stress (means: 18.8, 21.6 and 19.1). There were associations between: education, salary, multiple jobs and direct care with Compassion Satisfaction; low income, being a nurse and working overtime with Burnout; and working more than 12 hours, underlying disease and hospitalization for COVID-19 with Secondary Traumatic Stress. Conclusion: quality of life at work was satisfactory, despite the presence of Burnout and Secondary Traumatic Stress. Objective COVID19 COVID 19 COVID-1 cases Methods crosssectional cross sectional 15 hospital workrelated related regression Results mean (mean 38.2, 382 38.2 , 38 2 38.2) means (means 188 18 8 18.8 216 21 6 21. 19.1. 191 19.1 . 1 19.1) education salary income hours Conclusion satisfactory COVID1 COVID- 38. 3 18. 19.

ABSTRACT Purpose: Bacterial cellulose (BC) has shown high capacity for the treatment of wounds and burns, providing a moisty environment. Calcium alginate can be associated with BC to create gels that aid in wound debridement and contribute to appropriate wound healing. This study is aimed at characterizing and evaluating the use of bacterial cellulose/alginate gel in skin burns in rats. Methods: Cellulose and cellulose/alginate gels were compared regarding the capacity of liquid absorption, moisture, viscosity, and potential cytotoxicity. The 2nd degree burns were produced using an aluminum metal plate (2.0cm) at 120ºC for 20s on the back of rats. The animals were divided into non-treated, CMC(Carboxymethylcellulose), Cellulose(CMC with bacterial cellulose), and Cellulose/alginate(CMC with bacterial cellulose and alginate). The animals received topical treatment 3 times/week. Biochemical (MPO, NAG and oxidative stress), histomorphometry and immunohistochemical assays (IL-1β IL-10 and VEGF) were conducted on the 14th, 21st, 28th, and 35th days. Results: Cellulose/Alginate gel showed higher absorption capacity and viscosity compared to Cellulose gel, with no cytotoxic effects. Cellulose/alginate presented lower MPO values, a higher percentage of IL-10, with greater and balanced oxidative stress profile. Conclusions: The use of cellulose/alginate gel reduced neutrophils and macrophage activation and showed greater anti-inflammatory response, which can contribute to healing chronic wounds and burns. Purpose (BC environment cellulosealginate rats Methods moisture cytotoxicity nd 2.0cm 20cm cm 2 0cm (2.0cm ºC s nontreated, nontreated non treated, treated non-treated CMCCarboxymethylcellulose, CMCCarboxymethylcellulose CMC Carboxymethylcellulose , CMC(Carboxymethylcellulose) CelluloseCMC cellulose, cellulose) Cellulose/alginateCMC CellulosealginateCMC alginate. . alginate) timesweek times week times/week MPO, (MPO stress, stress) IL1β ILβ IL 1β β IL10 10 IL-1 VEGF 14th th 21st st 28th days Results CelluloseAlginate Alginate effects Cellulosealginate values IL10, 10, profile Conclusions antiinflammatory anti inflammatory response CMC(Carboxymethylcellulose alginateCMC IL1 1 IL-

ABSTRACT Objective: To address disparities in oral healthcare to allow prioritization of individuals with higher vulnerability. The model seeks to develop a methodology for identifying and classifying vulnerability among CEO users using socioeconomic indicators. Additionally, it aims to establish criteria for access prioritization based on vulnerability profiles, ultimately ensuring that those in greater need receive the specialized oral healthcare they require. Material and Methods: The proposed method leverages two key indicators: the Municipal Social Vulnerability Index (IVS) and the CEO Social Vulnerability Indicator (CEO IVS). CEO IVS is calculated based on CEO user socioeconomic data and compared to the municipality's IVS. The model utilizes a scoring system to classify users' vulnerability levels, allowing for the identification of those most in need of specialized care. The Equality Production Trend Indicator (ITPE-CEO) assesses the alignment between CEO and municipal vulnerability levels, aiding access prioritization. Conclusion: This equity-focused model offers a practical approach to address disparities in oral healthcare access. Vulnerability profiles and socioeconomic factors empower local managers to allocate resources effectively, ensuring that individuals with higher vulnerability receive timely and specialized care. The proposed model promotes inclusivity and responsiveness to community needs, contributing to achieving equitable oral healthcare for all. Objective indicators Additionally require Methods (IVS . IVS) municipalitys municipality s levels care ITPECEO ITPE (ITPE-CEO Conclusion equityfocused equity focused effectively needs all

Resumen Objetivo: comprender las percepciones sobre liderazgo de los enfermeros durante la pandemia de COVID-19 en hospitales universitarios brasileños, a través de la concepción de los conceptos del marco de John Kotter y su modelo de cambio de ocho pasos. Método: investigación multicéntrica de carácter cualitativo y diseño analítico. La muestra estuvo compuesta por 139 enfermeros que trabajaban durante la pandemia de COVID-19 entrevistados mediante una guía de entrevistas semiestructuradas; categorizadas a través del análisis de contenido de Bardin basándose en los conceptos de John Kotter y su modelo de cambio de ocho pasos. Resultados: los hallazgos revelaron importantes percepciones de los enfermeros sobre el liderazgo en la lucha contra el COVID-19, que presentó características consistentes con los preceptos de Kotter. Se observa un liderazgo basado en la búsqueda del conocimiento y una comunicación respetuosa, que facilita el trabajo en equipo por medio de una relación de confianza y respeto. Se reconoce lo complejo y difícil que es ejercer el liderazgo como enfermero, especialmente en contextos de crisis. Conclusión: las percepciones de los enfermeros destacan elementos esenciales para la práctica del liderazgo, como la importancia de adquirir múltiples conocimientos, tener una comunicación respetuosa, relaciones basadas en la confianza y el respeto, y reconocer la complejidad del liderazgo, que representa las características de los preceptos de Kotter. Objetivo COVID19 COVID 19 COVID-1 brasileños pasos Método analítico 13 semiestructuradas Resultados COVID19, 19, respetuosa respeto enfermero crisis Conclusión conocimientos COVID1 1 COVID-

Resumo Objetivo: conhecer as percepções de liderança de enfermeiros durante a pandemia da COVID-19 em hospitais universitários brasileiros, pela concepção dos conceitos do referencial de John Kotter e seu modelo de mudança de oito etapas. Método: pesquisa multicêntrica de natureza qualitativa e delineamento analítico. A amostra foi constituída por 139 enfermeiros atuantes na COVID-19 entrevistados mediante um roteiro de entrevistas semiestruturadas, categorizadas por meio de análise de conteúdo de Bardin com base nos conceitos de John Kotter e seu modelo de mudança de oito etapas. Resultados: os achados resultaram em importantes percepções dos enfermeiros acerca da liderança durante o enfrentamento da COVID-19, que apresentaram características condizentes com os preceitos de Kotter. Evidencia-se uma liderança baseada na busca pelo conhecimento, pautada em uma comunicação polida, facilitando o trabalho em equipe por meio de uma relação de confiança e respeito. O reconhecimento da complexidade e dificuldade que é exercer a liderança do enfermeiro, principalmente, em contextos de crise. Conclusão: as percepções dos enfermeiros reforçam elementos essenciais para a prática da liderança, como a importância pela busca de conhecimentos múltiplos, comunicação polida, relação baseada na confiança e respeito, e o reconhecimento da complexidade em liderar, apresentando, assim, características acerca dos preceitos de Kotter. Objetivo COVID19 COVID 19 COVID-1 brasileiros etapas Método analítico 13 semiestruturadas Resultados COVID19, 19, Evidenciase Evidencia se conhecimento polida respeito enfermeiro principalmente crise Conclusão múltiplos liderar apresentando assim COVID1 1 COVID-

Abstract Objective: to understand nurses’ leadership perceptions during the COVID-19 pandemic in Brazilian university hospitals, through the lens of John Kotter’s concepts and his eight-step change model. Method: a multicenter qualitative research with an analytical design. The sample comprised 139 nurses working in COVID-19 settings, interviewed using a semi-structured interview guide. Data were categorized through Bardin’s content analysis based on John Kotter’s concepts and his eight-step change model. Results: the findings yielded significant insights into nurses’ perceptions of leadership during the COVID-19 response, which exhibited characteristics consistent with Kotter’s principles. There is evidence of leadership based on the pursuit of knowledge, grounded in polished communication, facilitating teamwork through a relationship of trust and respect. The recognition of the complexity and difficulty of exercising nursing leadership, particularly in crisis contexts, is apparent. Conclusion: nurses’ perceptions reinforce essential elements for leadership practice, such as the importance of seeking diverse knowledge, polished communication, relationships based on trust and respect, and recognition of the complexity of leadership, thus presenting characteristics in line with Kotter’s principles. Objective COVID19 COVID 19 COVID-1 hospitals Kotters Kotter s eightstep eight step model Method design 13 settings semistructured semi structured guide Bardins Bardin Results response principles knowledge communication respect contexts apparent Conclusion practice COVID1 1 COVID-

Abstract Climate change is a global concern, with far-reaching implications for biodiversity and ecosystems. Understanding impact on species distribution is crucial for effective conservation strategies. The aims of this study were to evaluate the projected effects of climate change on the potential distribution of Manihot species endemic to Northeast Brazil and estimate the presence of climate suitability within protected areas in the future. We used ecological niche models to assess the potential distribution of 11 endemic species, providing predictions of current and future scenarios using an optimistic and pessimistic climate change scenario. The results revealed that in the optimistic scenario, 45% of the species may experience a partial reduction in their potential distribution range by 2100, and this percentage increases to 54% in the pessimistic scenario. Other species, on the other hand, will increase their potential distribution. The climatically suitable area for most species will be inserted in some protected areas, but species with limited current distribution and decreasing potential range must be prioritized for conservation. This study provides valuable information about the future potential distribution of endemic species of Manihot. concern farreaching far reaching ecosystems strategies 1 scenario 45 2100 54 hand 4 210 5 21 2