Abstract Introduction: The pandemic has had a broad and lasting impact on the Macau community, which has motivated many scholars to explore these effects from different perspectives. In our view, it would have special meaning to conduct a study from the perspective of the linguistic landscape. Objective: Investigate how the COVID-19 pandemic has affected Macau's linguistic landscape. Methods: Our research was essentially a quantitative study through a comparative analysis of specific data collected before and during the pandemic. Results: The results showed that linguistic diversity in Macau was maintained, in parallel with the increase in the predominance of the Chinese mother tongue of the majority of inhabitants. Non-governmental organizations and institutions were less affected by language policy in disseminating messages related to the pandemic. Conclusion: Macau's linguistic landscape transformed concomitantly with the changes caused by the pandemic, particularly in the demographic structure of the local community.

Resumen Introducción: La pandemia ha tenido un impacto amplio y duradero en la comunidad de Macao, lo que ha motivado a muchos académicos a explorar estos efectos desde diferentes perspectivas. En nuestra opinión, tendría especial significado realizar un estudio desde la perspectiva del paisaje lingüístico. Objetivo: Investigue cómo la pandemia de COVID-19 ha afectado el panorama lingüístico de Macao. Métodos: Nuestra investigación fue esencialmente un estudio cuantitativo mediante un análisis comparativo de datos específicos recopilados antes y durante la pandemia. Resultados: Los resultados mostraron que la diversidad lingüística en Macao se mantuvo, en paralelo con el aumento del predominio de la lengua materna china de la mayoría de los habitantes. Las organizaciones e instituciones no gubernamentales se vieron menos afectadas por la política lingüística a la hora de difundir mensajes relacionados con la pandemia. Conclusión: El panorama lingüístico de Macao se transformó al mismo tiempo que los cambios provocados por la pandemia, particularmente en la estructura demográfica de la comunidad local.

Resumo Introdução: A pandemia teve um impacto amplo e duradouro na comunidade de Macau, o que motivou muitos académicos a explorar esses efeitos sob diversas perspetivas. Na nossa visão, teria um significado especial conduzir um estudo sob a perspetiva da paisagem linguística. Objetivo: Investigar como a pandemia de COVID-19 tem tido impacto na paisagem linguística de Macau. Métodos: A nossa investigação foi essencialmente um estudo quantitativo por meio de uma análise comparativa de dados específicos recolhidos antes e durante a pandemia. Resultados: Os resultados mostraram que a diversidade linguística em Macau se mantinha, em paralelo ao aumento da predominância da língua materna chinesa da maioria dos habitantes. As organizações e instituições não governamentais foram menos afetadas pela política linguística na disseminação de mensagens relacionadas com a pandemia. Conclusão: A paisagem linguística de Macau transformou-se concomitantemente com as mudanças provocadas pela pandemia, nomeadamente na estrutura demográfica da comunidade local.

Abstract Objective: Reliably prediction models for coronary artery abnormalities (CAA) in children aged > 5 years with Kawasaki disease (KD) are still lacking. This study aimed to develop a nomogram model for predicting CAA at 4 to 8 weeks of illness in children with KD older than 5 years. Methods: A total of 644 eligible children were randomly assigned to a training cohort (n = 450) and a validation cohort (n = 194). The least absolute shrinkage and selection operator (LASSO) analysis was used for optimal predictors selection, and multivariate logistic regression was used to develop a nomogram model based on the selected predictors. Area under the receiver operating characteristic curve (AUC), calibration curves, Hosmer-Lemeshow test, Brier score, and decision curve analysis (DCA) were used to assess model performance. Results: Neutrophil to lymphocyte ratio, intravenous immunoglobulin resistance, and maximum baseline z-score ≥ 2.5 were identified by LASSO as significant predictors. The model incorporating these variables showed good discrimination and calibration capacities in both training and validation cohorts. The AUC of the training cohort and validation cohort were 0.854 and 0.850, respectively. The DCA confirmed the clinical usefulness of the nomogram model. Conclusions: A novel nomogram model was established to accurately assess the risk of CAA at 4–8 weeks of onset among KD children older than 5 years, which may aid clinical decisionmaking. Objective (CAA (KD lacking Methods 64 n 450 194. 194 . 194) (LASSO AUC, , (AUC) curves HosmerLemeshow Hosmer Lemeshow test score (DCA performance Results ratio resistance zscore z 25 2 2. cohorts 0854 0 854 0.85 0850 850 0.850 respectively Conclusions 48 4– decisionmaking 6 45 19 (AUC 085 85 0.8 1 08 0.

ABSTRACT Introduction: The aim of this study was to identify perioperative risk factors of laryngeal symptoms and to develop an implementable risk prediction model for Chinese hospitalized patients undergoing coronary artery bypass grafting (CABG). Methods: A total of 1476 Chinese CABG patients admitted to Wuhan Asian Heart Hospital from January 2020 to June 2022 were included and then divided into a modeling cohort and a verification cohort. Univariate analysis was used to identify laryngeal symptoms risk factors, and multivariate logistic regression was applied to construct a prediction model for laryngeal symptoms after CABG. Discrimination and calibration of this model were validated based on the area under the receiver operating characteristic (ROC) curve and the Hosmer-Lemeshow (H-L) test, respectively. Results: The incidence of laryngeal symptoms in patients who underwent CABG was 6.48%. Four independent risk factors were included in the model, and the established aryngeal complications risk calculation formula was Logit (P) = −4.525 + 0.824 × female + 2.09 × body mass index < 18.5 Kg/m2 + 0.793 × transesophageal echocardiogram + 1.218 × intensive care unit intubation time. For laryngeal symptoms, the area under the ROC curve was 0.769 in the derivation cohort (95% confidence interval [CI]: 0.698-0.840) and 0.811 in the validation cohort (95% CI: 0.742-0.879). According to the H-L test, the P-values in the modeling group and the verification group were 0.659 and 0.838, respectively. Conclusion: The prediction model developed in this study can be used to identify high-risk patients for laryngealsymptoms undergoing CABG, and help clinicians implement the follow-up treatment. Introduction . (CABG) Methods 147 202 (ROC HosmerLemeshow Hosmer Lemeshow HL H L (H-L test respectively Results 648 6 48 6.48% P (P 4525 4 525 −4.52 0824 0 824 0.82 209 2 09 2.0 185 18 5 18. Kgm2 Kgm Kg m2 m Kg/m 0793 793 0.79 1218 1 218 1.21 time 0769 769 0.76 95% 95 (95 CI [CI] 0.6980.840 06980840 0.698 0.840 698 840 0.698-0.840 0811 811 0.81 0.7420.879. 07420879 0.742 0.879 742 879 0.742-0.879) Pvalues values 0659 659 0.65 0838 838 0.838 Conclusion highrisk high followup follow up treatment (CABG 14 20 64 6.48 452 52 −4.5 082 82 0.8 2. 079 79 0.7 121 21 1.2 076 76 9 (9 [CI 6980 0.6980.84 0698084 0698 0.69 0840 0.84 69 84 0.698-0.84 081 81 7420 0.7420.879 0742087 0742 0.74 0879 0.87 74 87 0.742-0.879 065 65 0.6 083 83 0.83 6.4 45 −4. 08 8 0. 07 7 12 1. ( 0.6980.8 069808 069 084 0.698-0.8 0.7420.87 074208 074 087 0.742-0.87 06 6. −4 0.6980. 06980 0.698-0. 0.7420.8 07420 0.742-0.8 − 0.6980 0.698-0 0.7420. 0.742-0. 0.698- 0.7420 0.742-0 0.742-

Natural killer (NK) cells are a critical component of the innate immune system and one of the immune cells most sensitive to exercise. So far, it is widely believed that moderate exercise can significantly enhance the proliferation and activity of NK cells, strengthening immune function. However, the impact of exercise on NK cells is a dynamic and complex process. In addition to the type of exercise, the frequency, intensity, and duration of exercise are also key factors. This article not only briefly summarizes the activation mechanisms of NK cells but also delves into the potential importance of exercise as a non-pharmacological strategy in modulating NK cell activity and enhancing the immune system. Emerging studies have indicated that the timing and regularity of exercise bouts might also influence NK cell responses. Moreover, the interaction between exercise and other components of the immune system, such as cytokines and chemokines, could further modulate the functionality of NK cells. The above research is of crucial significance for achieving a deeper understanding of the intricate connection between exercise and NK cell function, as well as the development of effective health promotion strategies. In addition, further research is needed to investigate the effects of long-term exercise on NK cell function and the interaction between exercise and NK cell-mediated immune responses. Translating these research findings into precisely tailored exercise programs for specific populations, taking into account factors like age, health status, and genetic predisposition, could potentially offer unprecedented prospects for further advancements in this burgeoning field of study. (NK far However process frequency intensity nonpharmacological non pharmacological responses Moreover chemokines strategies longterm long term cellmediated mediated populations age status predisposition study

Abstract This article presents a corpus of Chinese learners of Portuguese L2 with PoS and lemma annotations, highlighting its potential for quantitative and qualitative analysis in identifying linguistic patterns among learners, thus contributing to the teaching of Portuguese L2. This corpus (University of Macau Portuguese Learners Corpus), named UMPLC, contains a total of 933 compositions produced by 122 Portuguese students from University of Macau over three consecutive years of study. PoS and lemma annotation was performed using Stanza, an automatic annotator developed by Qi et al. ( 2020 ). To ensure annotation consistency, the results were manually reviewed. In this research, the PoS and lemma information enables us to quantitatively and qualitatively investigate various phenomena in the corpus relating to lexical aspects and diachronic changes in this regard. Two studies were conducted based on a contrastive approach, comparing the Portuguese of learners in the corpus with native Portuguese. Non-native linguistic characteristics were discovered, allowing Portuguese L2 teachers to focus on areas requiring corrective work. L annotations Corpus, Corpus , Corpus) UMPLC 93 12 study Stanza al 202 . ) consistency reviewed research regard approach Nonnative Non discovered work 9 1 20 2

Resumo O presente artigo apresenta um corpus de aprendizes chineses de português L2 com a anotação de PoS e lema, destacando-se sua potencialidade de análise quantitativa e qualitativa na identificação de padrões linguísticos dos aprendizes, contribuindo, dessa forma, para o ensino de português L2. Este corpus (Corpus de Aprendizes de Português da Universidade de Macau), denominado UMPLC, contém, no total, 933 composições produzidas por 122 estudantes de português da Universidade de Macau durante três anos de estudo consecutivos. A anotação de PoS e lema realizou-se através do Stanza, anotador automático desenvolvido por Qi et al. ( 2020 ). A fim de garantir a consistência de anotação, o resultado foi revisado manualmente. Nesta pesquisa, as informações de PoS e lema permitem-nos investigar quantitativa e qualitativamente diversos fenômenos existentes no corpus relativos ao aspeto lexical e à mudança diacrônica desse aspeto. Dois estudos foram realizados com base em uma abordagem contrastiva, comparando-se o português dos aprendizes do corpus com o português nativo. Foram descobertas características de não-natividade linguística desses aprendizes, o que permitirá que os professores de português L2 se concentrem nas áreas em que é necessário um trabalho corretivo. L destacandose destacando contribuindo forma Corpus Macau, , Macau) UMPLC contém total 93 12 consecutivos realizouse realizou Stanza al 202 . ) manualmente pesquisa permitemnos permitem nos contrastiva comparandose comparando nativo nãonatividade não natividade corretivo 9 1 20 2

Jun N-terminal kinase pathway-associated phosphatase (JKAP) regulates CD4+ T-cell differentiation and immunity, which are linked to mental disorders. This study aimed to explore the relationships between JKAP and T helper 17 (Th17)/regulatory T (Treg) ratio, as well as their associations with anxiety and depression in postpartum women. Serum JKAP were measured by enzyme-linked immunosorbent assay and blood Th17 and Treg cells were measured by flow cytometry in 250 postpartum women. Anxiety and depression were evaluated by the 6-item State-Trait Anxiety Inventory (STAI6) and Edinburgh Postnatal Depression Scale (EPDS). Anxiety and depression rates were 22.0 and 28.4%, respectively, among postpartum women. Notably, JKAP was negatively associated with the STAI6 (P=0.002) and EPDS scores (P<0.001) in postpartum women and was lower in postpartum women with anxiety (P=0.023) or depression (P=0.002) than in those without. Moreover, JKAP was inversely related to Th17 cells and Th17/Treg ratio but positively correlated with Treg cells in postpartum women (all P<0.001). Interestingly, Th17 cells and Th17/Treg ratio were both positively associated with STAI6 and EPDS scores in postpartum women (all P<0.001). Furthermore, Th17 cells and Th17/Treg ratio were lower in postpartum women with anxiety or depression than in those without (all P<0.01). Nevertheless, Treg cells were not linked to anxiety or depression in postpartum women. JKAP was negatively associated with Th17 cells and Th17/Treg ratio; moreover, they all related to anxiety and depression in postpartum women, indicating that JKAP may be involved in postpartum anxiety and depression via interactions with Th17 cells. Nterminal N terminal pathwayassociated pathway (JKAP CD4 CD Tcell cell immunity disorders 1 Th17/regulatory Th17regulatory Thregulatory /regulatory regulatory Th (Treg enzymelinked enzyme Th1 25 6item item 6 StateTrait State Trait STAI (STAI6 EPDS. . (EPDS) 220 22 0 22. 284 28 4 28.4% respectively Notably P=0.002 P0002 P 002 (P=0.002 P<0.001 P0001 001 (P<0.001 P=0.023 P0023 023 (P=0.023 Moreover Th17Treg ThTreg P<0.001. P<0.001) Interestingly Furthermore P<0.01. P001 P<0.01 01 P<0.01) Nevertheless moreover 2 (STAI (EPDS 28.4 P=0.00 P000 00 (P=0.00 P<0.00 (P<0.00 P=0.02 P002 02 (P=0.02 P00 P<0.0 28. P=0.0 (P=0.0 (P<0.0 P0 P<0. P=0. (P=0. (P<0. P<0 P=0 (P=0 (P<0 P< P= (P= (P< (P

ABSTRACT This study was conducted to investigate the polymorphism of the silence information regulator 5 (SIRT5) gene in 103 Congjiang Xiang pigs from Southwest China. We searched for SNP (single nucleotide polymorphism) loci of SIRT5 gene through sequence alignment and PCR. We obtained nine SNP loci: g.14135 A>C (intron 6), g.14247 C>A (intron 6), g.14305 C>T (exon 7), g.14335 C>T (exon 7), g.16603 T>C (intron 7), g.16613 T>C (intron 7), g.16800 G>A (intron 7), g.16812 C>G (intron 7), and g.16916 A>G (exon 8). Further analysis of SNP genotypes associated with the carcass traits of skin thickness, backfat thickness, and eye muscle area was carried out in pigs. We found that the genotypes g.14305 C>T (CC) and g.16812 C>G (CG) had certain advantages for improving the carcass traits of Congjiang Xiang pigs. The haplotype combination of the SIRT5 gene that improved skin thickness was H2H3:CCGGCTCA. These results may provide empirical support for molecular-based breeding of carcass traits in Congjiang Xiang pigs. SIRT (SIRT5 10 China single PCR g14135 g 14135 g.1413 AC A C intron 6, 6 , 6) g14247 14247 g.1424 CA g14305 14305 g.1430 CT T exon 7, 7 7) g14335 14335 g.1433 g16603 16603 g.1660 TC g16613 16613 g.1661 g16800 16800 g.1680 GA G g16812 16812 g.1681 CG g16916 16916 g.1691 AG 8. 8 . 8) CC (CC (CG H2H3CCGGCTCA. H2H3CCGGCTCA HHCCGGCTCA H2H3 CCGGCTCA. CCGGCTCA H H2H3:CCGGCTCA molecularbased molecular based (SIRT 1 g1413 1413 g.141 g1424 1424 g.142 g1430 1430 g.143 g1433 1433 g1660 1660 g.166 g1661 1661 g1680 1680 g.168 g1681 1681 g1691 1691 g.169 HH H2H g141 141 g.14 g142 142 g143 143 g166 166 g.16 g168 168 g169 169 g14 14 g.1 g16 16 g1 g.

Abstract Objective: Continuous research on the structure and function of intestinal microecology has confirmed the association between gut microbiota and the occurrence, development, and outcome of allergic diseases. Here, we explored the genetic causality between gut microbiota and rhinitis. Methods: We conducted a two-sample Mendelian Randomization (MR) study to investigate the genetic causal relationship between gut microbiota and allergic rhinitis and vasomotor rhinitis. Genetic variations in the human gut microbiota were obtained from the summary statistics of the MiBioGen study. Genome-wide summary statistics of rhinitis were obtained from the FinnGen consortium. The causal effect between gut microbiota and rhinitis was assessed using the inverse variance weighted, MR-Egger regression, and weighted median methods. In addition, sensitivity analyses were conducted using different methods, including maximum likelihood, simple mode, and weighted model methods. Results: The IVW approach revealed a causal association of the genus Ruminococcus gauvreauii group with an increased risk of allergic rhinitis (IVW Odds Ratio [OR = 1.26] [1.04, 1.53], p-value = 0.01645). In addition, the genus Fusicatenibacter (IVW OR = 1.20 [1.02, 1.41], p-value = 0.02868) was causally associated with an increased risk of vasomotor rhinitis. Conclusion: Gut microbiota belonging to different genera exert different effects on allergic rhinitis and vasomotor rhinitis, including reducing the risk of rhinitis, and increasing the risk of rhinitis. New insights into the mechanisms of underlying gut microbiota-associated rhinitis are provided. Level of evidence: Level 5. Objective occurrence development diseases Here Methods twosample two sample MR (MR Genomewide Genome wide consortium MREgger Egger regression methods addition likelihood mode Results 1.26 126 1 26 1.04, 104 04 [1.04 1.53, 153 1.53 , 53 1.53] pvalue p value 0.01645. 001645 0.01645 . 0 01645 0.01645) 120 20 1.2 1.02, 102 02 [1.02 1.41, 141 1.41 41 1.41] 0.02868 002868 02868 Conclusion microbiotaassociated provided evidence 5 12 2 1.04 10 [1.0 15 1.5 00164 0.0164 0164 1. 1.02 14 1.4 4 0.0286 00286 0286 1.0 [1. 0016 0.016 016 0.028 0028 028 [1 001 0.01 01 0.02 002 [ 00 0.0 0.

Abstract Studies have reported that >91.9% of non-syndromic tooth agenesis cases are caused by seven pathogenic genes. Objective To report novel heterozygous PAX9 variants in a Chinese family with non-syndromic oligodontia and summarize the reported genotype-phenotype relationship of PAX9 variants. Methodology We recruited 28 patients with non-syndromic oligodontia who were admitted to the Hospital of Stomatology Hebei Medical University (China) from 2018 to 2021. Peripheral blood was collected from the probands and their core family members for whole-exome sequencing (WES) and variants were verified by Sanger sequencing. Bioinformatics tools were used to predict the pathogenicity of the variants. SWISS-MODEL homology modeling was used to analyze the three-dimensional structural changes of variant proteins. We also analyzed the genotype-phenotype relationships of PAX9 variants. Results We identified novel compound heterozygous PAX9 variants (reference sequence NM_001372076.1) in a Chinese family with non-syndromic oligodontia: a new missense variant c.1010C>A (p.T337K) in exon 4 and a new frameshift variant c.330_331insGT (p.D113Afs*9) in exon 2, which was identified as the pathogenic variant in this family. This discovery expands the known variant spectrum of PAX9; then, we summarized the phenotypes of non-syndromic oligodontia with PAX9 variants. Conclusion We found that PAX9 variants commonly lead to loss of the second molars. 919 91 9 >91.9 nonsyndromic non syndromic genes PAX genotypephenotype genotype phenotype 2 China (China 201 2021 wholeexome whole exome WES (WES SWISSMODEL SWISS MODEL threedimensional three dimensional proteins reference NM_001372076.1 NM0013720761 NM 001372076 1 c1010CA cCA c 1010C A C p.T337K pT337K pTK p T337K T K (p.T337K c330331insGT cinsGT 330 331insGT insGT p.D113Afs*9 pD113Afs9 pDAfs D113Afs D Afs (p.D113Afs*9 then molars >91. 20 202 NM_001372076. NM001372076 00137207 CA pT TK 33 p.D113Afs* pD pD113Afs DAfs (p.D113Afs* >91 NM_001372076 NM00137207 0013720 3 p.D113Afs (p.D113Afs >9 NM_00137207 NM0013720 001372 > NM_0013720 NM001372 00137 NM_001372 NM00137 0013 NM_00137 NM0013 001 NM_0013 NM001 00 NM_001 NM00 0 NM_00 NM0 NM_0 NM_

ABSTRACT This paper discusses the design of a safflower picking robot control system and focuses on a navigation control subsystem based on multisensor fusion. A navigation subsystem, an identification and positioning subsystem, a picking subsystem, and a levelling subsystem are designed. The hardware and software of the navigation subsystem are designed in detail, and a multisensor fusion positioning method based on extended Kalman fusion technology is proposed. The accuracy and stability levels of different combined navigation methods are compared. To test the effectiveness and accuracy of the proposed method, an outdoor test is carried out. The test results show that the outdoor fusion positioning accuracy of the robot is less than 8 cm, and when the satellite signal is lost, the navigation control subsystem can still provide high positioning accuracy. The final positioning result obtained using the integrated positioning method of the wheel odometer + IMU + DGNSS is approximately 52% higher than that of the odometer, approximately 29% higher than that of the wheel odometer + IMU, and approximately 11% higher than that of the IMU + DGNSS. detail compared out cm lost 52 29 11 5 2 1

ABSTRACT To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpression was achieved in the cells with transfection of the recombinant lentivirus strategies. Txnip silencing promoted the differentiation of porcine preadipocytes and PPARγ expression, and a PPARγ inhibitor reduced this facilitation. Instead, Txnip overexpression exerted a suppressive effect on the cell differentiation and PPARγ expression, and the PPARγ agonist offset this inhibition. High glucose stimulated the preadipocyte differentiation and expressions of ChREBP and Txnip. In contrast, Txnip expression was reduced by ChREBP silencing, suggesting glucose-regulated Txnip expression through the mediation of ChREBP. Moreover, the expressions of ChREBP and Glut4 induced by high glucose and glucose uptake of the cells were reduced by Txnip-overexpression, but increased by Txnip silencing, while these changes of Txnip did not alter their expressions under low glucose. Collectively, Txnip could be an inhibitor of porcine preadipocyte differentiation, which attenuated the adipogenesis through the negative feedback regulation on PPARγ. Txnip impaired the induction of glucose on the preadipocyte differentiation through decreasing Glut4 expression and glucose uptake and subsequent decrease of the expression and transcriptional activity of ChREBP. threedayold three day old silenced strategies facilitation Instead inhibition contrast glucoseregulated regulated Moreover Glut Txnipoverexpression, Txnipoverexpression overexpression, Txnip-overexpression Collectively

RESUMEN Objetivos: Se estudiaron los efectos de la antigravedad en el hígado utilizando un modelo de ratón macho en prueba de suspensión de la cola (TS, tail suspension). Métodos: Se examinaron los efectos de la TS a las 0, 2 y 4 semanas (grupos CON, TS2 y TS4) sobre el contenido de glucógeno y lípidos, así como sobre los procesos moleculares de las vías de síntesis y degradación. Resultados: (1) El número de glucogenosomas ultraestructurales y el contenido de glucógeno fueron expresivamente más altos en el grupo TS4 que en los otros dos grupos. (2) En el grupo TS4, la actividad de la glucógeno sintasa se mantuvo constante, mientras que la actividad de la glucógeno fosforilasa disminuyó, lo que indica que la degradación del glucógeno se redujo. (3) Los hígados del grupo TS2 presentaron el mayor contenido de lípidos y triglicéridos, lo que indica la acumulación de lípidos en el hígado en ese momento. (4) En el grupo TS2, la actividad de los factores relacionados con la síntesis de ácidos grasos acetil-CoA carboxilasa y ácido graso sintasa aumentó, mientras que la lipasa hepática disminuyó, indicando que la síntesis de lípidos aumentó mientras que la descomposición disminuyó. (5) En el grupo TS2, la expresión proteica de los transportadores de glucosa 1 y 2 aumentó. Conclusiones: Desde la semana TS2 hasta la semana TS4, el principal modo de consumo de energía en el hígado de los ratones pasó del metabolismo de la glucosa al metabolismo de los lípidos a medida que disminuía el uso de la glucosa. Nivel de Evidencia II, Estudio retrospectivo comparativo. Objetivos TS, (TS suspension. suspension . suspension) Métodos 0 grupos CON Resultados (1 (2 constante disminuyó redujo 3 (3 triglicéridos momento (4 acetilCoA acetil CoA 5 (5 Conclusiones II comparativo (

ABSTRACT Objectives: The effects of weightlessness on the liver were studied using a tail suspension (TS) male mouse model. Methods: The effects of 0-, 2- and 4-week TS (CON, TS2 and TS4 groups) on glycogen and lipid content, as well as on the molecular processes of the synthesis and degradation pathways, were examined. Results: (1) The number of glycogenosomes under ultrastructure and the glycogen content were considerably larger in the TS4 group than in the other two groups. (2) In the TS4 group, glycogen synthase activity remained constant while glycogen phosphorylase activity dropped, indicating that glycogen breakdown was reduced. (3) The livers of the TS2 group had the highest lipid and triglyceride content, indicating lipid buildup in the liver at this time. (4) In the TS2 group, the activities of the fatty acid synthesis-related factors acetyl-CoA carboxylase and fatty acid synthase increased, while hepatic lipase decreased, indicating that lipid synthesis increased, while decomposition decreased. (5) In the TS2 group, the protein expression of glucose transporters 1 and 2 increased. Conclusions: From TS2 weeks to TS4 weeks, the main energy consumption mode in the livers of mice transitioned from glucose metabolism to lipid metabolism as glucose use decreased. Level of evidence II; Comparative prospective study. Objectives (TS model Methods 0, 0 , 0- 4week week 4 CON, CON (CON groups pathways examined Results (1 (2 dropped reduced 3 (3 time (4 synthesisrelated related acetylCoA acetyl CoA increased decreased 5 (5 Conclusions II study (

RESUMO Objetivos: Os efeitos da antigravidade no fígado foram estudados usando um modelo de camundongo macho com a suspensão pela cauda (TS, tail suspension). Métodos: Foram examinados os efeitos da TS em 0, 2 e 4 semanas (grupos CON, TS2 e TS4) sobre o conteúdo de glicogênio e lipídios, bem como nos processos moleculares das vias de síntese e degradação. Resultados: (1) O número de glicogenossomos ultraestruturais e o teor de glicogênio foram expressivamente maiores no grupo TS4 do que nos outros dois grupos. (2) No grupo TS4, a atividade de glicogênio sintase permaneceu constante, enquanto a atividade de glicogênio fosforilase caiu, indicando que a degradação do glicogênio foi reduzida. (3) Os fígados do grupo TS2 tiveram o maior teor lipídico e de triglicérides, indicando acúmulo de lipídios no fígado no momento. (4) No grupo TS2, a atividade dos fatores relacionados com a síntese de ácidos graxos acetil-CoA carboxilase e ácido graxo sintase aumentaram, enquanto a lipase hepática diminuiu, indicando que a síntese de lipídios aumentou, enquanto a decomposição diminuiu. (5) No grupo TS2, a expressão proteica dos transportadores de glicose 1 e 2 aumentou. Conclusões: De TS2 semanas para TS4 semanas, o principal modo de consumo de energia no fígado de camundongos passou do metabolismo da glicose para o metabolismo lipídico, à medida que o uso de glicose diminuiu. Nível de evidência II, Estudo retrospectivo comparativo. Objetivos TS, (TS suspension. suspension . suspension) Métodos 0 grupos CON Resultados (1 (2 constante caiu reduzida 3 (3 triglicérides momento (4 acetilCoA acetil CoA aumentaram diminuiu aumentou 5 (5 Conclusões II comparativo (

Abstract The image of an athlete's ankle joint injury can help to check whether the athlete's ankle joint is damaged, and plays a very important role in clinical diagnosis. To address the problem of poor segmentation effect of traditional athletes' ankle injury image segmentation algorithm, an ankle injury image segmentation algorithm based on residual double attention U-Net model is proposed. First, the region of interest is extracted from the original ankle injury image. After translation, rotation and turnover, the image data is expanded. Second, the residual structure is used to adjust the gradient propagation and residual feedback of the segmentation framework, extract the attribute information in the region of interest, and combine the two to retain more image features. Finally, combined with the double attention module to improve the weight ratio of image features, the athlete ankle injury image segmentation is realized in the image segmentation framework based on residual double attention U-Net model. The results demonstrate that the maximum values of DSC, ASSD, PM, and CR for the proposed algorithm are 0.93, 0.1, 0.96, and 0.95, respectively, and the F1 score is 95.7%, indicating that the segmentation effect of this algorithm is closer to the theoretical segmentation effect, and higher precision in segmentation, and the segmented image has a high degree of similarity to the original image, resulting in excellent segmentation performance. athletes s damaged diagnosis UNet U Net First translation turnover expanded Second features Finally DSC ASSD PM 093 0 93 0.93 01 1 0.1 096 96 0.96 095 95 0.95 respectively F 957 7 95.7% performance 09 9 0.9 0. 95.7 95.

Precipitation interpolation is widely used to generate continuous rainfall surfaces for hydrological simulations. However, increasing the precision of values at the unknown points generated by different spatial interpolation methods is challenging. This study used the Chaohe River Basin, which is an important source of Beijing's drinking water, as a research area to comprehensively evaluate several precipitation interpolation methods (Thiessen polygon, inverse distance weighting, ordinary kriging and ANUSPLIN) for inputs in hydrological simulations. This research showed that the precipitation time-series surface generated using the ANUSPLIN interpolation method had higher accuracy and reliability. Using this precipitation input to drive the hydrological models, we explored the parameter uncertainties of four typical hydrological models (GR4J, IHACRES, Sacramento and MIKE SHE) based on the multi-objective generalized likelihood uncertainty estimation (GLUE) method. The GLUE method was used to study the parameter sensitivity and uncertainty of the model. Results showed that the ANUSPLIN precipitation interpolation surface combined with the Sacramento model performed best. The multi-objective GLUE method had obvious advantages in parameter uncertainty analysis in hydrological models. Simultaneously exploring the convex line and point density distributions of the behavioural parameters with multi-objective functions determined their distribution and sensitivity more effectively.

Abstract Objective: Liver cancer is the fifth most common cancer in the world. Research on the pathogenesis and detailed molecular mechanisms of liver cancer is very important. The immune system plays an important role in regulating the incidence and metastasis of liver cancer. Materials and methods: This work collected 20 blood samples from patients with clinical hepatocellular carcinoma without metastasis, 20 blood samples from patients with metastatic hepatocellular carcinoma, and 20 blood samples from healthy subjects. Flow cytometry was used to analyze the content of Treg and Th2 cells in the three groups of blood samples. Immunofluorescence was applied to analyze the relative expression of CTLA-4 and CD28 in lymphocytes of each group of blood samples. Western blot was used to analyze the T cell surface protein CTLA-4, CD28, GATA3, and FOXP3 expression in each group of blood samples. Results: The expression of CD28 and GATA3 in the blood of patients with hepatocellular carcinoma without metastasis was obviously higher than that of patients with metastasis of hepatocellular carcinoma, which is contrary to the expression trend of CTLA-4 and FOXP3, and corresponds to the content ratio of Treg and Th2 cells, thus verifying the relationship between Treg/Th2 ratio and metastasis of hepatocellular carcinoma. Conclusions: In the microenvironment of liver cancer, the ratio of Treg/Th2 will increase significantly, thereby promoting the metastasis of hepatocellular carcinoma.

Resumen Objetivo: El cáncer de hígado es el quinto cáncer más común en el mundo. La investigación sobre la patogenia y los mecanismos moleculares detallados del cáncer de hígado es muy importante. El sistema inmunológico juega un papel importante en la regulación de la incidencia y metástasis del cáncer de hígado. Material y métodos: Este trabajo recogió 20 muestras de sangre de pacientes con carcinoma hepatocelular clínico sin metástasis, 20 muestras de sangre de pacientes con carcinoma hepatocelular metastásico y 20 muestras de sangre de sujetos sanos. Se utilizó citometría de flujo para analizar el contenido de células Treg y Th2 en los tres grupos de muestras de sangre. Se aplicó inmunofluorescencia para analizar la expresión relativa de CTLA-4 y CD28 en linfocitos de cada grupo de muestras de sangre. Se utilizó Western blot para analizar la expresión de la proteína de superficie de células T CTLA-4, CD28, GATA3, FOXP3 en cada grupo de muestras de sangre. Resultados: La expresión de CD28 y GATA3 en la sangre de pacientes con carcinoma hepatocelular sin metástasis fue obviamente mayor que la de pacientes con metástasis de carcinoma hepatocelular, lo cual es contrario a la tendencia de expresión de CTLA-4 y FOXP3, y corresponde al contenido relación de células Treg y Th2, verificando así la relación entre la relación Treg/Th2 y la metástasis del carcinoma hepatocelular. Conclusiones: En el microambiente del cáncer de hígado, la proporción de Treg/Th2 aumentará significativamente, promoviendo así la metástasis del carcinoma hepatocelular.